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1.
盐旱耦合胁迫对费尔干猪毛菜种子萌发及其幼苗生长的影响 总被引：1，自引：0，他引：1
本研究采用双因素组合实验,探讨盐(NaCl)、干旱(PEG 6000)对费尔干猪毛菜(Salsola ferganica)种子萌发和幼苗生长的影响。结果显示:①费尔干猪毛菜种子萌发能耐受较高浓度NaCl和PEG胁迫。低浓度NaCl或PEG胁迫下,费尔干猪毛菜去翅种子和具翅种子萌发率与对照无显著差异,高浓度NaCl或PEG处理下,去翅种子萌发率35%以上,显示较强的耐盐、耐旱性;盐旱耦合处理下,不同低浓度PEG能够显著缓解中浓度NaCl对种子萌发的抑制作用。②随NaCl浓度升高,具翅种子恢复萌发率逐渐上升,而去翅种子则维持较低水平;随着PEG浓度的升高,2种种子恢复萌发能力显著降低;盐旱耦合处理下2种种子的恢复萌发率与NaCl胁迫具有相同趋势。③NaCl或PEG对去翅种子幼苗的影响比具翅种子强,特别是PEG处理;随NaCl或PEG浓度升高,2种种子幼苗的株高和根长均降低;盐旱耦合处理下低浓度NaCl对去翅种子幼苗的影响比具翅种子小(除了100 mmol·L^-1NaCl+25 g·L^-1PEG处理),中浓度NaCl对去翅种子幼苗的影响比具翅种子强,高浓度NaCl处理下随PEG浓度升高,2种种子幼苗的株高和根长均降低。以上结果显示,费尔干猪毛菜在萌发期和幼苗期具有较强的耐盐耐旱性,盐旱耦合在一定程度上能够缓解盐离子毒害效应,这对其度过早期恶劣环境具有重要的生态学意义;果翅对种子在胁迫下进入次级休眠,从而补充土壤种子库起到重要作用。 相似文献
2.
运用培养皿滤纸法和盆栽法,分别研究温度、光照和水分条件对热带常见杂草丰花草种子萌发和幼苗生长的影响。结果表明:丰花草种子萌发温度范围广泛(5~40℃),随温度升高,丰花草幼苗的鲜质量和生长长度均呈现先升高后降低的趋势;恒温下30℃为其种子萌发的最适温度,其发芽率、发芽指数、活力指数均相对较高,而平均发芽时间最短;25℃时生长的幼苗最健壮,其芽长、根长和芽鲜质量、根鲜质量均显著高于其他温度时的对应值。而变温条件更有利于丰花草种子萌发,12 h D/12 h L、5℃/15℃时发芽率达55.63%,15℃/25℃时发芽率90%,25℃/35℃时的种子萌发率、幼苗长度、幼苗鲜质量均最大。光照不是影响丰花草种子萌发的关键因子,但光照可能有利于促进丰花草幼苗生物量向根部分配,光暗交替(12 h D/12 h L)条件下丰花草幼苗生长情况相对最好(长度根冠比为1.05∶1;鲜质量根冠比为0.51∶1)。水分是决定丰花草种子能否萌发的关键因素,25℃、12 h D/12 h L条件下,在土壤最大持水量60%时丰花草种子萌发和幼苗生长状况均最佳。 相似文献
3.
微生境对于脆弱生态系统的植被更新与恢复至关重要。为了探明侵蚀微环境对植物更新的影响,以黄土丘陵沟壑区裸坡为对照,选取细沟、浅沟沟坡、浅沟沟底3种侵蚀微环境,结合7种主要物种种子补播试验,通过2 a的萌发出苗、幼苗存活与生长监测,结果表明:除铁杆蒿幼苗存活率在裸坡高于各侵蚀微环境外,其他供试物种的种子萌发出苗率和存活率在细沟或浅沟环境明显高于裸坡,而且大部分植物种子萌发出苗率和幼苗存活率在细沟内最高;供试的7种物种幼苗的高度在裸坡不同程度地低于细沟、或浅沟沟底、或浅沟沟坡下,尤其在浅沟沟底的白羊草、长芒草和杠柳高度显著高于其他环境(P0.05)。各侵蚀微环境下,不同坡位对供试物种的种子萌发出苗率、幼苗存活率与高度的影响没有一致性规律。在黄土丘陵沟壑区,土壤侵蚀塑造的侵蚀微环境对大部分植物种子萌发出苗、幼苗存活和生长等更新关键过程具有一定的优势,其中,细沟更有利于植物种子萌发出苗及幼苗存活,而浅沟更有利于植物幼苗生长。然而,土壤侵蚀微地形对植物更新也存在一定的种间差异性,细沟和浅沟不利于种子极小的铁杆蒿种子萌发出苗及幼苗存活。合理管理与利用侵蚀微环境,对该区恶劣生境的植被恢复具有实际意义。 相似文献
4.
阿拉善荒漠一年生植物种子萌发特性及生态适应性分析 总被引：1，自引：0，他引：1
对阿拉善荒漠地区主要的一年生植物种子萌发特性和生态适应进行了测定和分析,结果表明:三芒草(Aristida adscenionis)、雾冰藜(Bassia dasyphylla)、猪毛菜(Salsola collina)的种子当年萌发率较高,均超过70%,干、冷藏处理过冬后的萌发率略有下降,但仍超过60%;狗尾草(Setaria viridis)、虎尾草(Chloris virgata)、冠芒草(Pappophorum boreale)种子当年萌发率均在15%以下,狗尾草干藏处理后萌发率下降、虎尾草、冠芒草种子干冷藏处理后,萌发率大幅度上升,表现明显的休眠后熟现象;小画眉草(Eragrostis minor)种子当年萌发率接近30%,但处理后萌发率几乎降至0;虫实(Corispermum declinatum)、灰绿藜(Chenopodium glaucum)种子各种处理的萌发率都在15%以下,特别是灰绿藜种子当年不萌发。与草原区相比,荒漠区一年生植物种子萌发率偏低,萌发进程较缓慢,表现出对荒漠气候的适应性。 相似文献
5.
用不同浓度的NaCl溶液对花棒(heydeysarum scoparium)种子进行胁迫萌发实验,观察其发芽率和萌发后种苗的生长情况,并测定萌发吸水率及淀粉酶活性.结果表明:花棒种子萌发对盐胁迫有一定的适应性,当NaCl盐浓度≤0.9%时,种子的发芽率均能达到90%以上;但随着NaCl浓度的增加,种子的萌发吸水受到抑制,淀粉酶活性降低,发芽率、发芽指数和活力指数均呈逐渐下降趋势.当NaCl盐浓度≤0.6%时,种子萌发出的幼苗生长基本不受影响;但当NaCl盐浓度≥1.5%时,胚根伸长基本停止. 相似文献
6.
盐分、温度对猪毛菜种子萌发的影响 总被引：2，自引：4，他引：2
通过对浆果猪毛菜和钠猪毛菜种子在不同盐分、温度环境下的萌发过程与对策研究,结果表明:2种猪毛菜种子萌发的最佳温度约为25 ℃.浆果猪毛菜在蒸馏水中的萌发速度低于钠猪毛菜.浓度低于0.2 mol/L的NaC1溶液对萌发的影响不大;随NaCl浓度增高,浆果猪毛菜和钠猪毛菜种子萌发率降低.一定的盐分胁迫不影响种子萌发潜力,其萌发恢复率随原处理溶液抑制程度的增加而增加.同时,讨论了2种猪毛菜种子萌发特性与环境的关系. 相似文献
7.
以天然生长于祁连山自然保护区隆畅河自然保护站的濒危植物蒙古扁桃(Prunus mongolica)去内果皮的种子为材料,以不同渗透势PEG-6000溶液模拟干旱条件,研究了种子萌发和幼苗生长对干旱胁迫的响应特征。结果表明,随着干旱胁迫程度的加剧,蒙古扁桃种子吸胀速率、萌发率、萌发指数、活力指数、苗高和根长、组织饱和含水量等指标均表现出明显降低的趋势,而幼苗初生芽干重、根干重和根冠比均呈先升后降的趋势。蒙古扁桃种子对水势变化既敏感又抗旱,种子能够萌发的最低渗透势阈值为-0.65 MPa。干旱胁迫未能萌发的种子复水后萌发率较高。分析认为,蒙古扁桃种子萌发和幼苗生长对干旱胁迫的响应特征对种的延续具有重要意义,但在人工栽培时保证
土壤墒情应是保障建植成功的关键措施。 相似文献
8.
聚乙二醇胁迫下赤霉素浸种对番茄种子萌发和幼苗生长的影响 总被引：3，自引：0，他引：3
以番茄幼苗为材料,研究了50 mg/g 的赤霉素(GA3)和清水浸种后用不同浓度的聚乙二醇(0、40、80、120 mg/g)胁迫对番茄种子萌发及幼苗生长的影响.结果表明:40 mg/g的聚乙二醇胁迫对番茄种子的萌发率、发芽指数、幼苗株高、根长有促进作用,120 mg/g的聚乙二醇明显抑制种子的萌发率、发芽指数、幼苗株高、根长;不同浓度的聚乙二醇胁迫明显降低了幼苗的叶绿素含量.用50 mg/g GA3浸种后明显增加聚乙二醇胁迫下番茄种子的萌发率、发芽指数、幼苗株高、根长和叶绿素含量.用50 m g/g GA3浸种对番茄幼苗的生长有一定的促进作用. 相似文献
9.
NaCl胁迫对草木樨种子萌发及幼苗生长的影响 总被引：1，自引：0，他引：1
采用室内种子萌发及温室幼苗培养实验方法,研究不同浓度NaCl胁迫对耐盐植物草木樨种子萌发及幼苗生长的影响,结果表明：低浓度的NaCl胁迫可以提高种子的萌发率、累积发芽率、发芽势,增加幼苗地上部分的鲜干比、叶绿素含量,并促进幼苗根和芽的生长,而高浓度的NaCl胁迫则对其有显著的抑制作用;不同浓度的NaCl胁迫对草木樨种子的发芽指数均有一定的抑制作用,而对幼苗叶片的丙二醛含量却有一定的促进作用。因此得出：低浓度的NaCl胁迫对草木樨种子萌发及幼苗生长具有一定的促进作用,而高浓度的NaCl胁迫则对其有一定的抑制作用。 相似文献
10.
以朝牧1号谷稗种子为试材,采用不同剂量(50、100、150、200、250、300、350、400、450 Gy)的60Co-γ射线辐照其种子,测定种子的发芽率、发芽势、发芽指数和活力指数,芽苗的主根长、芽长、芽苗总长、鲜重、干重和相对含水量,种子的出苗率和幼苗成苗率,并对种子萌发期各指标进行相关性和主成分分析。结果表明,随着辐射剂量的增加,中高辐射剂量发芽率降低了3.34%~25.00%,发芽势增加了6.84%~41.09%,发芽指数增加了1.84~33.50%,活力指数降低了8.87%~62.86%,芽长降低了9.97%~56.78%,主根长降低了18.48~70.52%,芽苗总长降低了11.25~62.20%,鲜重（除150 Gy外）降低了0.32%~34.50%,干重降低了2.91%~11.58%,相对含水量增加了29.37%~57.54%,50~350 Gy处理出苗率增加了3.12%~18.75%,50~300 Gy处理成苗率降低了5.13%~94.87%。不同剂量60Co-γ射线辐射后,发芽率、活力指数、芽长、主根长、芽苗总长、鲜重均与成苗率呈极显著正相关,以特征向量值大于0.30为标准,辐射影响谷稗种子萌发和幼苗生长主要指标分别为芽长、芽苗总长、主根长、成苗率、活力指数、发芽率、相对含水量和出苗率。 相似文献
11.
MAMUT Jannathan 《干旱区科学》2019,11(5):774-784
For a plant species to complete its life cycle in arid and saline environments, each stage of the life cycle must be tolerant to the harsh environmental conditions. The aim of the study was to determine the effects of water stress (water potentials of -0.05, -0.16, -0.33, -0.56, -0.85 and -1.21 MPa) and NaCl stress (50, 100, 200, 300, 400, 500 and 600 mmol/L NaCl) on seed germination percentage, seedling survival and growth, juvenile growth and plant reproduction of Lachnoloma lehmannii Bunge (Brassicaceae), an cold desert annual that grows in the Junggar Basin of Xinjiang, China in 2010. Results indicated that low water stress (-0.05 and -0.16 MPa) had no significant effect on seed germination percentage. With a decrease in water potential, germination percentage decreased, and no seeds germinated at -0.85 and -1.21 MPa water stresses. Germination percentage of seeds was significantly affected by NaCl stress, and higher germination percentages were observed under non-saline than saline conditions. An increase in NaCl concentrations progressively inhibited seed germination percentage, and no seeds germinated at ≥400 mmol/L NaCl concentration. Non-germinated seeds were transferred from both PEG (polyethylene glycol-6000) and NaCl solutions to distilled water for seed germination recovery. The number of surviving seedlings and their heights and root lengths significantly decreased as NaCl stress increased. About 30% of the plants survived and produced fruits/seeds at 200 mmol/L NaCl concentration. Thus, seed germination, seedling establishment and reproductive stage in the life cycle of L. lehmannii are water- and salt-tolerant, with seedlings being the least tolerant. These tolerances help explain why this species can survive and produce seeds in arid and saline habitats. 相似文献
12.
Zahra JAFARI 《干旱区科学》2020,12(3):495-507
Scarce and scattered precipitation in arid regions is detrimental for newly planted seedlings. It is essential to provide required water storage for seedlings in restoration projects in the first year of their establishment. The subsurface irrigation can be much more effective than the surface irrigation because of the regulation of water availability and reduction in water evaporation. We studied the effect of surface and subsurface irrigation methods on the growth and survival of four common tree species including heaven tree(Ailanthus altissima(Mill.) Swingle), China berry(Melia azedarach L.), white mulberry(Morus alba L.), and black locust(Robinia pseudoacacia L.) by installing underground clay reservoirs with different permeabilities in Isfahan City, Iran. Different amounts of animal manure and wheat straw were mixed with clay fraction and cooked in a pottery kiln at 900°C to produce reservoirs with different permeabilities. The experimental treatments consisting of irrigation and tree species were considered with a factorial arrangement in a completely randomized design with three replications in 2016 and 2017. Leaf water potential of seedlings, which is indirectly related to drought resistance, was measured by a portable pressure chamber. The results showed that saplings height, basal diameter, number of leaves, chlorophyll content and stomatal conductance were significantly(P0.05) higher in the subsurface irrigation with low permeability than in the surface irrigation, but the number of branches of the studied species were not significantly(P0.05) affected by the irrigation methods and different permeabilities of clay reservoirs. The clay reservoirs with low and medium permeabilities constantly provide better conditions for plant growth, and water with lower pressure and longer time intervals to the plant roots as compared with the reservoirs with high permeability. Analysis of variance of the data showed that year and interaction between year and permeability of reservoir had significant effects(P0.05) on all growth parameters, except for the chlorophyll content. In addition, the highest percentage of survival was 100% associated with the subsurface irrigation and the control treatment had the lowest survival percentages of 60%, 70%, 80% and 100% for M. alba, M. azedarach, A. altissima and R. pseudoacacia, respectively. Finally, the values of leaf water potential showed that R. pseudoacacia was the most drought resistant species. 相似文献
13.
草本层是古尔班通古特沙漠植被的重要组成部分,在维持沙漠稳定、养分循环等方面具有重要意义。水分通常以积雪和降雨形式输入到土壤,输入方式及数量变化对荒漠植物的存活和生长有重要影响。本文以古尔班通古特沙漠南缘草本层为研究对象,利用人工增减积雪的方法,共设置4个积雪处理(0,50%,100%,200%积雪,其中100%积雪为对照),于2009—2016年春季调查草本植物的数量特征和物种丰富度,分析草本植物数量特征对水分变化的响应;同时结合气象数据,分析不同年份一年生荒漠草本植物数量特征与主要气象因子的关系。结果表明：表层土壤含水量与积雪厚度成正比,相应的草本植物的幼苗密度与积雪水当量、表层土壤含水量成正比关系;2009—2016年,年内积雪量的变化对物种丰富度没有显著影响,各积雪处理间物种丰富度差异不显著;2009—2016年自然处理下年际间物种丰富度差异显著,2015年物种丰富度显著小于其余年份。结合气象数据分析发现,物种丰富度年际变化主要受幼苗建成期的降雨调控,并且干旱年份过后的湿润年份物种丰富度不受前一年降水的影响,说明荒漠草本植物层片具有较强的自我调节能力;自然处理下,不同年份草本植物旺盛期的存活数量与大气干旱程度(空气饱和差)呈显著负相关关系（R2值为0.611,P<0.05）,与生长季的降雨量呈正相关关系（R2值为0.162, P>0.05）,说明大气干旱程度更能表征荒漠草本植物生长峰值期的存活情况。 相似文献
14.
水杨酸对水稻幼苗抗瘟性的诱导作用 总被引：51，自引：3，他引：51
网室试验证明,水杨酸(SA)叶面喷雾可减轻水稻幼苗稻瘟病的发生。在平板培养中,SA对稻瘟菌分生孢子的萌发及菌丝生长均无明显抑制作用,因此认为SA处理后稻瘟病病情指数的下降是由于SA处理提高了水稻幼苗的抗瘟性,即稻苗产生了诱导抗性而引起的。SA诱发水稻幼苗产生对苗叶瘟诱导抗性的浓度为0.01~0.1mM。SA喷雾处理后2~4天接种,诱导抗性表现最强。0.01mMSA在接种前2天喷雾处理三叶一心期稻苗一次后,水稻叶片的病斑数目和大小比清水对照分别降低72.7%和55.4%,病指比对照降低59.8%,抗瘟性的持久期为15天(病指降低24.3%)。经0.01mMSA诱导处理后再用同浓度SA进行一次强化处理可增强诱抗效果,延长抗性持久期。用0.01mMSA处理水稻第一和第二叶可使未经处理的第三和第四叶上产生系统性抗瘟性。 相似文献
15.
儿茶素在棉苗对枯萎病抗性中的作用 总被引：3，自引：0，他引：3
分析了枯萎病菌接种后棉苗组织内儿茶素含量的变化以及儿茶素对枯萎病菌及其多聚半乳糖醛酸酶活性的影响。健康棉苗组织中儿茶素含量随着棉苗的生长而增加。枯萎病菌接种后,病苗组织中儿茶素含量显著高于健康对照,而且抗病品种棉苗儿茶素含量的增加明显高于感病品种。经氟乐灵预处理的棉苗接种枯萎病菌后,儿茶素含量增加更快,增幅更大。说明儿茶素是品种抗性和氟乐灵诱发棉苗对枯萎病诱导抗性的机理之一。儿茶素可抑制枯萎病菌的菌丝生长、产孢及孢子萌发,对培养液中病菌的多聚半乳糖醛酸酶(PG)有抑制作用。儿茶素化学制品及棉苗组织提取液经TLC分离后获得的含儿茶素组分提取液对初步纯化的PG活性有明显的抑制作用。这些结果表明:儿茶素可能通过抑制病菌的菌丝生长、产孢以及其PG活性而在棉花对枯萎病的抗病性中起到作用。 相似文献
16.
Climate change is shifting the amount and frequency of precipitation in many regions, which is expected to affect seedling recruitment across ecosystems. However, the combined effects of precipitation amount and frequency on seedling recruitment remain largely unknown. An understanding of the effects of precipitation amount and frequency and their interaction on seedling emergence and growth of typical desert plants is vital for managing populations of desert plants. We conducted two experiments to study the effects of variation in precipitation on Reaumuria soongarica(Pall.) Maxim. First, greenhouse experiments were conducted to examine the effects of three precipitation amount treatments(ambient, +30%, and-30%) and two precipitation frequency treatments(ambient and-50%) on seedling emergence. Second, the morphological responses of R. soongarica to changes in precipitation amount and frequency were tested in a controlled field experiment. Stage-specific changes in growth were monitored by sampling in different growth seasons. Our results showed that precipitation amount significantly affected germination, seedling emergence, and growth of R. soongarica, and there was a larger effect with decreased precipitation frequency compared with ambient. Germination and seedling emergence increased as precipitation increased under the same frequency of precipitation. The highest emergence percentage was obtained with a 30% increase in precipitation amount and a 50% reduction in precipitation frequency. Compared with ambient precipitation, a 30% increase in precipitation amount increased above-and below-ground biomass accumulation of R. soongarica during the growth season. A decrease of 30% in precipitation amount also resulted in an increase in below-ground biomass and root/shoot ratio in the early stages of the growth season, however, above-and below-ground biomass showed the opposite results at the end of the growth season, with larger effects on above-ground than below-ground biomass under decreased precipitation frequency. When precipitation frequency decreased by 50%, values of all growth traits increased for a given amount of precipitation. We concluded that precipitation frequency may be as important as precipitation amount to seedling emergence and growth of R. soongarica, and that understanding the effects of precipitation variability on seedling recruitment requires the integration of both precipitation amount and frequency. In particular, the combination of a 30% increase in precipitation amount and 50% reduction in precipitation frequency increased the emergence and growth of seedlings, suggesting that alteration of amount and frequency of precipitation caused by climate change may have significant effects on seedling recruitment of R. soongarica. 相似文献
17.
LB-1为新筛选的生防近缘毛壳(Chaetomium subaffine)菌株。为明确LB-1培养液的抑病促生效果,本研究以黄瓜为供试植物,分别采用灌根和叶面喷施的方式,测定了LB-1培养液对黄瓜枯萎病和黄瓜白粉病的抑制效果;通过种子萌发、盆栽苗和生化检测试验,分析了LB-1培养液对黄瓜的促生作用。结果发现,LB-1培养液对黄瓜枯萎病抑制作用效果甚微,但对黄瓜白粉病生防效果明显,黄瓜叶片接菌24 h后叶面喷施LB-1培养液对白粉病的防效高达48.86%。LB-1培养液浸润催芽处理24 h的黄瓜种子萌发率和根长均显著高于对照(P=0.05),且LB-1培养液浸种、灌根、叶面喷施处理均能促进黄瓜幼苗的生长发育。LB-1没有产嗜铁素、产氢氰酸、固氮和溶磷能力,但能够产生吲哚乙酸(Indoleacetic acid,IAA)。表明生防菌株LB-1培养液能有效抑制黄瓜白粉病的发生,促进黄瓜种子萌发和植株生长,而且其促生作用可能通过产生IAA来实现。 相似文献
18.
LB-1为新筛选的生防近缘毛壳(Chaetomium subaffine)菌株。为明确LB-1培养液的抑病促生效果,本研究以黄瓜为供试植物,分别采用灌根和叶面喷施的方式,测定了LB-1培养液对黄瓜枯萎病和黄瓜白粉病的抑制效果;通过种子萌发、盆栽苗和生化检测试验,分析了LB-1培养液对黄瓜的促生作用。结果发现,LB-1培养液对黄瓜枯萎病抑制作用效果甚微,但对黄瓜白粉病生防效果明显,黄瓜叶片接菌24 h后叶面喷施LB-1培养液对白粉病的防效高达48.86%。LB-1培养液浸润催芽处理24 h的黄瓜种子萌发率和根长均显著高于对照(P=0.05),且LB-1培养液浸种、灌根、叶面喷施处理均能促进黄瓜幼苗的生长发育。LB-1没有产嗜铁素、产氢氰酸、固氮和溶磷能力,但能够产生吲哚乙酸(Indoleacetic acid,IAA)。表明生防菌株LB-1培养液能有效抑制黄瓜白粉病的发生,促进黄瓜种子萌发和植株生长,而且其促生作用可能通过产生IAA来实现。 相似文献
19.
Cintia Vanesa LEDER 《干旱区科学》2022,14(5):550-560
In semi-arid lands, vegetation is distributed in shrub patches immersed in a less vegetated interpatch matrix. Grazing affects perennial grass seed bank through a decrease in seed rain and an increase in seed predation and soil compaction. Nevertheless, some species with anchorage mechanisms in their seeds might overcome this, such as Nassella tenuis (Phil.) Barkworth. This is an important species in grazing paddocks because it has an intermediate palatability and its relatively tolerant to grazing. These characteristics allow N. tenuis to increase its abundance in grazed sites. Our objective was to assess how grazing affects the key palatable species from seeds to seedlings: i.e., seed rain, soil seed bank, and seedling recruitment in different microsites along a windward-leeward transect across shrub canopy. We hypothesized that: (1) the negative effects of grazing on N. tenuis fructification are reflected in its seed rain, soil seed bank, and seedling recruitment, especially in interpatches; (2) Nassella tenuis seed rain reduction, soil compaction by cattle in grazed sites, and removal of seeds by wind decrease its soil seed bank, especially in microsites exposed to the predominant wind; and (3) the decrease in N. tenuis soil seed bank and cover increase in annual species in grazed sites have negative effects on its seedling recruitment, especially in microsites exposed to predominant wind. We placed seed traps, collected soil samples, and monitored seedling recruitment in different locations around shrub canopy to address our hypotheses. Also, we established a manipulative experiment in which we sow N. tenuis seeds and followed its recruitment in different microsites. We compared the seed rain, soil seed bank, natural seedling recruitment, and sown seeds recruitment of N. tenuis between grazed and ungrazed sites. We analyzed differences between microsites along a windward-leeward transect across shrubs patches. Seed rain and soil seed bank had the same density in patches and interpatches both in ungrazed and grazed sites. But seed rain was higher, and soil seed bank was lower in ungrazed sites than in grazed sites. Almost all under-canopy microsites showed greater soil seed bank abundance and natural seedling recruitment in ungrazed sites. Sown seeds recruitment was the same between grazed and ungrazed sites, but it showed protective effects of shrubs in leeward microsites under grazed sites. As a conclusion, seed rain and soil seed bank are complementary under grazed sites. 相似文献