共查询到19条相似文献，搜索用时 250 毫秒
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肝胆管细胞癌是来源于肝胆管上皮细胞的恶性肿瘤,在多物种中都有发生。笔者运用组织病理学和免疫组织化学的方法,对2例犬肝肿瘤进行了诊断。结果显示：肝肿瘤眼观均为圆球形白色肿块,组织病理学检查肿瘤组织由呈腺管状排列的瘤细胞构成,有的腺管中央有坏死脱落的细胞,有的有黏液样物质,腺管间以薄的纤维结缔组织分隔,瘤组织有大片的坏死。瘤细胞多呈卵圆形,细胞核大、卵圆形,核分裂象多见,瘤细胞的CK19阳性表达,而CK18和AFP呈阴性表达。根据组织病理学和免疫组织化学检查结果判断,2例犬肝肿瘤为肝胆管细胞癌,本研究为犬肝肿瘤诊断积累了病理学资料。 相似文献
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1只2.5岁雄性英短猫,就诊前10天出现呕吐、排便困难、粪便稀薄症状,经检查,患猫消瘦,体温38.5℃,呼吸频率、脉搏均正常,腹部可触及1个游离硬物,按压腹部无痛感。B型超声检查显示,腹中部有1个大小为4.41 cm×3.08 cm的肿物。为明确肿物性质、确定治疗方案,对患猫进行开腹探查,可见肠系膜有多个大小不等的球形肿物,手术切除肿物,分别进行细胞学、组织病理学及免疫组织化学检查。其中细胞学检查可见多种形状细胞,有分散排列的梭形细胞,有多角形体积较大的细胞,据此怀疑为间质来源的神经纤维瘤、胃肠道间质瘤或上皮来源的鳞状上皮癌。组织病理学检查可见肿瘤细胞排列呈巢状,肿瘤细胞为多边形或梭形,细胞大小不一,细胞核深染,可见多量病理性核分裂相,异型性较明显,肿瘤组织中心伴有坏死灶,具有低分化鳞状细胞癌特征。免疫组织化学染色显示,肿瘤细胞细胞角蛋白(CK)5/6阳性、DOG1阴性、S-100阴性、SMA阴性、波形蛋白(Vimentin)阴性,支持低分化鳞状细胞癌。根据病理结果建议术后化疗,但主人未配合,术后出院时各项指标均恢复正常,但应密切随访。 相似文献
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网状细胞肉瘤又称网状细胞增殖症，是淋巴网状系统的一种肿瘤。本病多见于鸡，猫也曾有过报道，国内在犬还未见报道，现介绍如下。１ 病史１只２岁北京狮子犬，在包皮表面有红枣大小的肿块，经手术切除后，送病理室作组织病理学和电镜检查。２ 肉眼观察患犬包皮表面有红枣大小的肿块，其大小为５－５ｃｍ×２－５ｃｍ，分叶，呈桑椹状，呈粉红色鱼肉样外观。质地柔软，脆弱，与周围组织界限明显，血管很丰富，一碰即出血，见图１。图１ 包皮表面的肿块（↑）３ 组织病理学变化肿瘤组织由大量形态和大小各异、呈圆形、梭形或星形的淋巴样… 相似文献
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本研究为了探讨恶性黑色素瘤的临床诊断及鉴别诊断要点,通过组织病理学检查和免疫组织化学染色方法对两例犬恶性黑色素瘤进行诊断。组织病理学检查发现瘤细胞排列紊乱,细胞主要呈梭形和上皮样两种细胞形态,可见瘤巨细胞、核分裂相,肿瘤细胞胞质中可见色素颗粒,细胞异型性明显,核仁较大,有丝分裂活跃,属于恶性黑色素瘤典型的组织学表现。免疫组织化学染色发现六种肿瘤标记物结果显示为CK（-）、Vimentin（+）、S-100（+）、CD34（-）、SMA-α（-）、KI-67（+）。通过组织病理学检查和免疫组化染色结果可以诊断为恶性黑色素瘤。 相似文献
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对1例京巴犬尸体进行剖检、肿瘤组织学检查、免疫组化分析。剖检发现,在乳腺、腹股沟、颈部、胸部、肺部和膈肌部位均发现大小不等的结节状肿瘤;组织学检查显示,肿瘤病变是由梭形细胞和透明细胞组成,肿瘤细胞呈梭形或多形性,胞浆透明或嗜酸性,胞核圆形、卵圆形或长梭形,呈分叶状或束状排列,核分裂象多见;免疫组化检测显示,肿瘤细胞的胞核对P63呈强阳性反应。根据病理组织学和免疫组化观察结果,确诊该犬为乳腺肌上皮癌病例。结果显示,乳腺肌上皮细胞在发生恶变之后,其免疫表型并未发生改变,其生物学特性更具有浸润和转移的能力。P63可以用来鉴别犬乳腺肌上皮癌,具有高度的敏感性和特异性。 相似文献
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1只12岁雄性松狮犬,因精神沉郁,颈背部有一个直径约4 cm的隆起,食欲不振,偶有咳嗽而就诊。该犬X射线检查结果无明显异常。颈背部皮下穿刺物镜检可见大量红细胞及少量增多的中性粒细胞。血细胞计数检查及血清生化指标检查结果显示,红细胞数、血红蛋白含量、血细胞比容及血小板总数均低于参考值。皮下异常增生组织呈暗紫红色,质地较硬,外有包囊包裹。异常增生组织H.E.染色镜检可见大量血管腔和血管裂隙,向中心分化程度逐渐降低,瘤细胞大量聚集,细胞核大且呈棕黄色。细胞呈梭形、多边形或卵圆形不等。免疫组织化学检测结果显示标志物Ki-67阳性染色率约为30%。综合相关检查结果,患犬确诊为皮肤血管肉瘤。说明犬皮肤血管瘤临床诊断手段主要有血清生化检测、胸腹腔影像学检查、非脏器型积液穿刺检查和组织病理学检查、免疫组织化学检查等,其中免疫组织化学检查准确、可靠,对患犬的确诊及后续治疗方案的选择至关重要。 相似文献
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Yener Z 《DTW. Deutsche tier?rztliche Wochenschrift》2001,108(7):315-316
Tumor mass was detected in the skeletal musculature of the tibia in a sheep slaughtered at abattoir. Histologically, the tumor cells were in a typical alveolar pattern, and showed a cartilaginous tissue differentiation in some areas of the tumor mass. This tumor examined by avidin-biotin-peroxidase method was found to be positive for myoglobin. The diagnosis of undifferentiated alveolar rhabdomyosarcoma was based on the results of histopathology and immunohistochemistry. 相似文献
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Jillian M. Athey Laura E. Rice Aja B. Harvey Kevin E. Washburn Aline Rodrigues-Hoffmann 《Journal of veterinary diagnostic investigation》2021,33(1):108
A 3-mo-old, female Boer goat was presented because of respiratory difficulties. Tachypnea and inspiratory dyspnea were noted during physical examination. Thoracic radiographs were unremarkable; however, upper airway and nasal passage radiographs revealed a soft tissue mass within the nasal passages. The patient underwent cardiorespiratory arrest and did not respond to resuscitation efforts during endoscopy. A large, pedunculated, semi-firm mass originated from the soft palate and obstructed 90% of the nasopharynx on autopsy. Histologically, the mass was composed of primitive cells that multifocally formed tubules and glomeruloid structures intermingled with areas of fusiform and blastemal cells. The neoplastic cells were positive for cytokeratin (tubular and glomeruloid cells), vimentin (fusiform population and blastemal cells), and Wilms tumor 1 protein (glomeruloid structures) on immunohistochemistry, consistent with a triphasic nephroblastoma. To our knowledge, nasopharyngeal nephroblastoma has not been reported previously in any species. 相似文献
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Andrew H. Schlueter Shannon D. Dehghanpir Bonnie Boudreaux Christopher Robinson Jose Cesar Menk P. Lima Ingeborg M. Langohr 《Journal of veterinary diagnostic investigation》2021,33(4):753
Mesotheliomas are uncommon neoplasms that arise from mesothelial cells in either the abdominal or thoracic cavities and are rarely diagnosed in cats. A 10-y-old spayed female domestic shorthair cat was presented to the Louisiana State University oncology service for evaluation of a large amount of abdominal effusion. Abdominal ultrasound identified a large mesenteric mass with numerous ill-defined nodules. An abdominocentesis was performed with cytologic and immunocytochemical findings consistent with a neoplastic effusion, with large clusters of epithelioid cells that exhibited strong cytoplasmic expression of pancytokeratin, vimentin, and Wilms tumor 1 antigens. Further testing was declined, and meloxicam was prescribed until the cat died 23 d after initial presentation. Upon postmortem examination, the omentum was contracted into a firm mass adhered to multiple organs and accompanied by numerous small white nodules throughout the abdominal cavity. On histopathology and immunohistochemistry, neoplastic cells were found throughout the abdominal cavity; 60–95% exhibited moderate-to-strong cytoplasmic immunoreactivity for cytokeratin, vimentin, and Wilms tumor 1 protein. The final diagnosis was an epithelioid mesothelioma. Our case illustrates the utility of cytology, immunocytochemistry, and its relation to histology and immunohistochemistry. We also reviewed the reported cases of feline mesothelioma. 相似文献
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Bertazzolo W Giudice C Dell'Orco M Caniatti M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2003,32(4):209-212
A 14-year-old female Welsh Terrier was presented for evaluation of multiple skin masses. Two of them were diagnosed as a follicular (epidermal) cyst and a sebaceous epithelioma by cytology and histopathology. The third mass was located in the subcutis adjacent to the cervical trachea. Clinical findings, thoracic radiography, and laboratory results were otherwise unremarkable. Cytologically, the cervical mass was characterized by the presence of round to polygonal cells with distinct cell borders, mild to moderate anisocytosis, round to oval eccentric nuclei with prominent nucleoli, and a variable amount of finely granular, eosinophilic cytoplasm. Differential diagnoses included endocrine/neuroendocrine tumors such as thyroid adenocarcinoma, medullary thyroid carcinoma, carotid body chemodectoma, and parathyroid carcinoma. The mass was removed surgically and submitted for histopathologic and immunohistochemical examination. Histologically, the mass was composed of rows and nests of densely packed cells separated by thin fibrovascular stroma. Neoplastic cells had moderate to large amounts of pale eosinophilic dusty or finely vacuolated cytoplasm and round to oval nuclei with finely dispersed chromatin and 1 or 2 small nucleoli. On immunohistochemistry, neoplastic cells were positive for chromogranin A, calcitonin, and neuron-specific enolase, and were negative for cytokeratin and thyroglobulin. The final diagnosis was medullary thyroid carcinoma, an uncommon endocrine tumor of dogs that has cytologic and histopathologic features similar to other endocrine and neuroendocrine tumors. Immunohistochemistry or immunocytochemistry should be performed to differentiate medullary thyroid carcinoma from other endocrine/neuroendocrine tumors of dogs. 相似文献
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猫的肾上腺皮质癌转移至脾在兽医临床非常罕见。在本报告中,描述了1例10岁田园猫,其症状表现为腹部疼痛,频繁呕吐,消瘦,有慢性肾上腺功能损伤病史,影像学检查脾肿大。对患猫进行脾摘除手术,并对摘除的组织进行组织病理学检查和免疫组化分析。病理组织学结果显示,肿瘤细胞具有丰富的脂质样空泡,细胞体积大,有丝分裂象多见,且肿瘤细胞已发生血管侵袭,细胞核浓染;免疫组织化学结果显示,synaptophysin、S-100和GATA4阳性表达。根据病理组织学和免疫组织化学及病史和实验室检查,最后诊断为肾上腺皮质癌的脾转移。首次报道了猫肾上腺皮质癌脾转移临床病例及病理学诊断,对于临床相关疾病诊疗有一定的参考价值和借鉴意义。 相似文献
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Sapierzyński R 《Polish journal of veterinary sciences》2010,13(4):661-668
The aim of this study was to evaluate the utility of immunocytochemistry in a standard veterinary practice and to determine the immunophenotype of tumor cells in cases of multicentric lymphoma in dogs by immunocytochemical analysis of fine-needle biopsy specimens. The study was performed on cytological samples collected from 54 dogs, in which multicentric lymphoma was recognised based on clinical data, cytology or cytology and histology, and follow-up information. Diagnosis of lymphoma was established according to the updated Kiel classification. Immunocytochemical assays were conducted using commercially available antibodies to the pan T-lymphocyte marker CD3 and B cell antigen receptor complex CD79 alpha. Among all animals examined B cell lymphoma was recognized in 42/54 (77.8%) of cases, while in the remaining 12/54 (22.2%) of dogs T cell lymphoma was recognized. In 11 animals with lymphoma recognized cytologically, in which an entire lymph node was obtained for histology, the results of routine cytology and immunocytochemistry fully corresponded with findings revealed by histology and immunohistochemistry. Immunocytochemistry can be successfully conducted in smears stored at room temperature for 24 hours without changes of staining results. It can be stated that application of standard cytopathological assessment in connection with immunocytochemistry of lymph nodes samples collected from dogs with lymphoma is a method of choice for establishing final diagnosis, and avoids the need for reexamination or collection of tissue samples for histopathology and immunohistochemistry during surgical procedures in ambiguous cases. 相似文献
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Choi US Jeong SM Kang MS Jung IS Kim DY Lee CW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):47-49
Abstract: An 18-month-old male Doberman Pinscher was referred to the Veterinary Medical Teaching Hospital of the College of Veterinary Medicine for an erythemic nodular mass on the right forelimb. The mass was diagnosed as cutaneous lymphoma, based on cytologic examination of a mass aspirate and histopathology. Using immunohistochemistry the neoplastic cells were positive for CD3 but negative for CD79a, E-cadherin, and pancytokeratin, confirming their origin as T lymphocytes. No tumor recurrence was noted 18 months after surgery. To our knowledge, this is the first report of a solitary nodular form of cutaneous lymphoma in a young dog. 相似文献
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Brown DE Thrall MA Getzy DM Weiser MG Ogilvie GK 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(4):118-123
Cytologic features of bone marrow, tissue, and abdominal fluid in seven cases of malignant histiocytosis in dogs are described, and histopathology, hematology, and serum biochemistry of the cases are reviewed. Diagnosis of malignant histiocytosis was confirmed by tissue morphology and immunohistochemistry; neoplastic cells in all cases had positive immunoreactivity to lysozyme. This stain can be used to definitively establish the diagnosis of malignant histiocytosis on cytology specimens as well as tissue sections. Cytologic findings included numerous pleomorphic, large, discrete mononuclear cells with abundant, lightly basophilic, vacuolated, granular cytoplasm. Nuclei were round to oval to reniform with marked anisocytosis and anisokaryosis; nucleoli were prominent. Mitotic figures, often bizarre, were occasionally seen. Multinucleated giant cells and phagocytosis of erythrocytes and leukocytes were prominent features in cytologic preparations in four cases. Four dogs were anemic, five dogs were thrombocytopenic, and three dogs were hypercalcemic. Breeds affected included Doberman Pinscher (1), Golden Retriever (2), Flat Coated Retriever (3), and mixed-breed dog (1). 相似文献