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1.
白羊草幼穗的组织培养   总被引:7,自引:0,他引:7  
白羊草幼穗在附加在2,4-D 1.0mg/L,水解酪蛋白500或1000mg/L的N6或MS培养基中,在25±1℃,暗光培养条件下,两周时形成0.5cm^2大小的愈伤组织块。愈伤组织的发生率为84%,培养3周后形成旺盛生长的的愈伤组织。其分化是在含有NAA0.5mg/L,KT1.0mg/L的MS培养基上或在附加有KT1.0mg/L、IAA0.4mg/L、BA1.0mg/L的N6培养基上2 ̄4周后  相似文献   

2.
播娘蒿幼蕾和花序轴的组织培养和再生植株研究   总被引:3,自引:0,他引:3  
播娘蒿幼小花蕾在附加有15mg/LNAA、05mg/L6-BA和01mg/LKT的MS培养基中,培养两周即形成05cm2大小的愈伤组织块,出愈率达83%。继续培养两周转至附加有2mg/L6-BA、05mg/LKT和02mg/LNAA的MS分化培养基上,2~3周后出现绿色芽点,继续培养2周即可得到分化小芽。播娘蒿花序轴在附加有2~6mg/L6-BA、05mg/LNAA的MS培养基中,培养5~6周也都能分化出芽,但最佳培养基为4mg/L6-BA、05mg/LNAA。分化小芽转至附加05mg/LNAA或05mg/LIBA的1/2MS生根培养基上培养2~3周均能形成完整植株。  相似文献   

3.
红三叶组织培养及再生植株   总被引:9,自引:2,他引:7  
孟玉玲  韩榕 《草业学报》1994,3(2):51-54
本文采用MS,B5和PC一L2作为基本培养基,在不同激素组合条件下对红三叶(Trifoliumpratense)子叶及下胚轴进行组织培养。在MS,B5和PC一L2附加2mg/L2,4-D和0.5mg/LBA的培养基上,子叶及下胚轴愈伤组织诱导率均在89%以上。愈伤组织继代在B5无机盐十三倍MS有机成分+3%蔗糖+0.5mg/L2,4-D+0.5mg/L6BA0.2mg/LNAA,+10mg/LVc的培养基上生长良好。愈伤组织在B5附加0.1mg/L6BA+0.2mg/LIBA的培养基上再生植株。下胚轴在MS附加2mg/L6BA+1mg/LNAA的培养基上直接分化形成丛生苗,转入无激素NS培养基上再生根。实验表明VE,Vc,PVP和活性碳对愈伤组织褐化有抑制作用,10mg/LVc抗褐化作用最好。  相似文献   

4.
碱茅幼穗的组织培养初报   总被引:2,自引:0,他引:2  
碱茅幼穗在附加有24-D15mg/L的MS培养基中,暗培养条件下两周时形成05cm2大小的愈伤组织块,其发生率为43%。培养3周后形成旺盛生长的肉黄色愈伤组织。愈伤组织的分化是在含KT10mg/L、NAA05mg/L的MS培养基上2~4周时出现绿色芽点,继续培养1~2周即可得到分化植株,将该植株移至不含激素的MS或N6培养基上时,光照条件下形成绿色分化植株。  相似文献   

5.
假俭草侧芽愈伤诱导和植株再生   总被引:3,自引:3,他引:0  
以中国优良品系E 126假俭草的侧芽为外植体建立高效的再生体系。试验结果显示,1)适宜于侧芽生长的培养基为MS+BAP2.0mg/L+NAA0.8 mg/L;2)在最佳诱导培养基MS+2,4 D1.0 mg/L+BAP0.1 mg/L上,侧芽愈伤诱导率达93%。较强的光照能提高愈伤组织的诱导率;3)在最佳分化培养基MS+KT2.0mg/L 上,绿苗分化率为12.6%;4)试管苗最佳生长培养基为MS+BAP2.0mg/L+NAA0.8 mg/L;5)在最佳生根培养基MS+NAA0.6mg/L 上,试管苗的生根率达98%,植株移栽成活率为94%。本试验为假俭草体细胞筛选和遗传转化提供依据。  相似文献   

6.
草麻黄的组织培养及植株再生   总被引:1,自引:0,他引:1  
卢萍  刘军 《中国草地》1998,(6):53-55
将草麻黄幼草节间幼茎部分作外植体,培养在附加KT0.054mg/l+2,4-D1.11mg/L的MS培养基中,可成功诱导出愈伤组织,愈伤组织分化出芽;同样的外本培养在附加BA3mg/l+IAA0.2mg/l的MS培养基中,诱导出大量不定芽;不同途径得到的芽转移到MS附加6-BA0.056mg/l+1.11mg/l2,4+11.11mg/l2,4-D的培养基后,可分化出不定根,从而形成完整植株。  相似文献   

7.
大赖草的组织培养及植株再生的研究   总被引:6,自引:0,他引:6  
王丽  邹明谦 《草业科学》1995,12(6):42-44
大刺草在成熟胚在含有2mg/L2,4-D的MS培养上形成颗粒状愈伤组织,减少培养基中的2,4-D浓度,愈伤组织分化出绿色的芽点。生根培养基为不含任何激素的MS培养基,有分化能力的愈伤组织可以在保持培养基(MS+2mg/L2,4-D+0.5mg/LBAP)上长期保存,利用这一培养程序可以快速,大量地繁殖大赖草,文章还对外源激素在植物组织培养中的作用进行了讨论。  相似文献   

8.
顿河红豆草下胚轴培养及其植株再生   总被引:2,自引:1,他引:1  
对顿河豆草5d龄无菌苗下胚轴愈伤组织诱导及其植株再生条件的研究结果表明,愈伤组织培养基为MS基础培养基,附加0.2mg/l2,4-D、1mg/lBAP和2mg/lNAA,pH5.8;分化培养我激素MS培养基;生根培养基为1/2MS或1/2LS+0.05mg/lNAA+0.8%琼脂培养基。其愈伤组织绝大多数生长良好,出愈率达96.8%。体细胞胚化率为25.3%,具有体细胞胚分化力的基因型占35%,生  相似文献   

9.
热研二号柱花草组织培养研究   总被引:8,自引:1,他引:7  
研究结果表明,利用热研二号柱花草无菌苗的子叶为外植体,在M2培养基(MS忝分+0.8%琼脂+3.0%蔗糖+1.0mg/L萘乙酸+4.0mg/L激动素,pH6.0)培养可诱导分化出愈伤组织和枝条。1月龄枝条在M7培养基(1/2MS基本成分+0.8%琼脂+1.0蔗糖+0.5mg/L萘乙酸+0.05%活性炭,pH6.0)培养可诱导生根,生根率60%,获得了人有根,茎,叶,的完整柱花组培植株。  相似文献   

10.
黑麦草幼穗培养直接成苗和微繁殖   总被引:7,自引:0,他引:7  
陈文品  杜丑新 《中国草地》1994,(3):65-67,54
本文研究了多花黑麦草幼穗离体培养直接成苗和微繁殖。结果表明,在MS培养基中附加0.5-1.0mg.L^-1或0.5mg.L^-12,4-D时幼穗成苗率较高,分别达48%和75%;幼穗可不切段直接培养;在MS培养基中附加2mg.L^-1BAP时微繁殖的效果最好。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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