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1.
白羊草幼穗的组织培养   总被引:7,自引:0,他引:7  
白羊草幼穗在附加在2,4-D 1.0mg/L,水解酪蛋白500或1000mg/L的N6或MS培养基中,在25±1℃,暗光培养条件下,两周时形成0.5cm^2大小的愈伤组织块。愈伤组织的发生率为84%,培养3周后形成旺盛生长的的愈伤组织。其分化是在含有NAA0.5mg/L,KT1.0mg/L的MS培养基上或在附加有KT1.0mg/L、IAA0.4mg/L、BA1.0mg/L的N6培养基上2 ̄4周后  相似文献   

2.
播娘蒿幼蕾和花序轴的组织培养和再生植株研究   总被引:3,自引:0,他引:3  
播娘蒿幼小花蕾在附加有15mg/LNAA、05mg/L6-BA和01mg/LKT的MS培养基中,培养两周即形成05cm2大小的愈伤组织块,出愈率达83%。继续培养两周转至附加有2mg/L6-BA、05mg/LKT和02mg/LNAA的MS分化培养基上,2~3周后出现绿色芽点,继续培养2周即可得到分化小芽。播娘蒿花序轴在附加有2~6mg/L6-BA、05mg/LNAA的MS培养基中,培养5~6周也都能分化出芽,但最佳培养基为4mg/L6-BA、05mg/LNAA。分化小芽转至附加05mg/LNAA或05mg/LIBA的1/2MS生根培养基上培养2~3周均能形成完整植株。  相似文献   

3.
红三叶组织培养及再生植株   总被引:9,自引:2,他引:7  
孟玉玲  韩榕 《草业学报》1994,3(2):51-54
本文采用MS,B5和PC一L2作为基本培养基,在不同激素组合条件下对红三叶(Trifoliumpratense)子叶及下胚轴进行组织培养。在MS,B5和PC一L2附加2mg/L2,4-D和0.5mg/LBA的培养基上,子叶及下胚轴愈伤组织诱导率均在89%以上。愈伤组织继代在B5无机盐十三倍MS有机成分+3%蔗糖+0.5mg/L2,4-D+0.5mg/L6BA0.2mg/LNAA,+10mg/LVc的培养基上生长良好。愈伤组织在B5附加0.1mg/L6BA+0.2mg/LIBA的培养基上再生植株。下胚轴在MS附加2mg/L6BA+1mg/LNAA的培养基上直接分化形成丛生苗,转入无激素NS培养基上再生根。实验表明VE,Vc,PVP和活性碳对愈伤组织褐化有抑制作用,10mg/LVc抗褐化作用最好。  相似文献   

4.
碱茅幼穗的组织培养初报   总被引:2,自引:0,他引:2  
碱茅幼穗在附加有24-D15mg/L的MS培养基中,暗培养条件下两周时形成05cm2大小的愈伤组织块,其发生率为43%。培养3周后形成旺盛生长的肉黄色愈伤组织。愈伤组织的分化是在含KT10mg/L、NAA05mg/L的MS培养基上2~4周时出现绿色芽点,继续培养1~2周即可得到分化植株,将该植株移至不含激素的MS或N6培养基上时,光照条件下形成绿色分化植株。  相似文献   

5.
假俭草侧芽愈伤诱导和植株再生   总被引:3,自引:3,他引:0  
以中国优良品系E 126假俭草的侧芽为外植体建立高效的再生体系。试验结果显示,1)适宜于侧芽生长的培养基为MS+BAP2.0mg/L+NAA0.8 mg/L;2)在最佳诱导培养基MS+2,4 D1.0 mg/L+BAP0.1 mg/L上,侧芽愈伤诱导率达93%。较强的光照能提高愈伤组织的诱导率;3)在最佳分化培养基MS+KT2.0mg/L 上,绿苗分化率为12.6%;4)试管苗最佳生长培养基为MS+BAP2.0mg/L+NAA0.8 mg/L;5)在最佳生根培养基MS+NAA0.6mg/L 上,试管苗的生根率达98%,植株移栽成活率为94%。本试验为假俭草体细胞筛选和遗传转化提供依据。  相似文献   

6.
草麻黄的组织培养及植株再生   总被引:1,自引:0,他引:1  
卢萍  刘军 《中国草地》1998,(6):53-55
将草麻黄幼草节间幼茎部分作外植体,培养在附加KT0.054mg/l+2,4-D1.11mg/L的MS培养基中,可成功诱导出愈伤组织,愈伤组织分化出芽;同样的外本培养在附加BA3mg/l+IAA0.2mg/l的MS培养基中,诱导出大量不定芽;不同途径得到的芽转移到MS附加6-BA0.056mg/l+1.11mg/l2,4+11.11mg/l2,4-D的培养基后,可分化出不定根,从而形成完整植株。  相似文献   

7.
大赖草的组织培养及植株再生的研究   总被引:6,自引:0,他引:6  
王丽  邹明谦 《草业科学》1995,12(6):42-44
大刺草在成熟胚在含有2mg/L2,4-D的MS培养上形成颗粒状愈伤组织,减少培养基中的2,4-D浓度,愈伤组织分化出绿色的芽点。生根培养基为不含任何激素的MS培养基,有分化能力的愈伤组织可以在保持培养基(MS+2mg/L2,4-D+0.5mg/LBAP)上长期保存,利用这一培养程序可以快速,大量地繁殖大赖草,文章还对外源激素在植物组织培养中的作用进行了讨论。  相似文献   

8.
顿河红豆草下胚轴培养及其植株再生   总被引:2,自引:1,他引:1  
对顿河豆草5d龄无菌苗下胚轴愈伤组织诱导及其植株再生条件的研究结果表明,愈伤组织培养基为MS基础培养基,附加0.2mg/l2,4-D、1mg/lBAP和2mg/lNAA,pH5.8;分化培养我激素MS培养基;生根培养基为1/2MS或1/2LS+0.05mg/lNAA+0.8%琼脂培养基。其愈伤组织绝大多数生长良好,出愈率达96.8%。体细胞胚化率为25.3%,具有体细胞胚分化力的基因型占35%,生  相似文献   

9.
热研二号柱花草组织培养研究   总被引:8,自引:1,他引:7  
研究结果表明,利用热研二号柱花草无菌苗的子叶为外植体,在M2培养基(MS忝分+0.8%琼脂+3.0%蔗糖+1.0mg/L萘乙酸+4.0mg/L激动素,pH6.0)培养可诱导分化出愈伤组织和枝条。1月龄枝条在M7培养基(1/2MS基本成分+0.8%琼脂+1.0蔗糖+0.5mg/L萘乙酸+0.05%活性炭,pH6.0)培养可诱导生根,生根率60%,获得了人有根,茎,叶,的完整柱花组培植株。  相似文献   

10.
黑麦草幼穗培养直接成苗和微繁殖   总被引:7,自引:0,他引:7  
陈文品  杜丑新 《中国草地》1994,(3):65-67,54
本文研究了多花黑麦草幼穗离体培养直接成苗和微繁殖。结果表明,在MS培养基中附加0.5-1.0mg.L^-1或0.5mg.L^-12,4-D时幼穗成苗率较高,分别达48%和75%;幼穗可不切段直接培养;在MS培养基中附加2mg.L^-1BAP时微繁殖的效果最好。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

13.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

14.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

15.
16.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

17.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

18.
A knowledge of the microbiological status of milk and of the different structures in the mammary glands has great importance in elucidating the pathogenesis of mammary gland infections. The objective of this study was to evaluate the microbiological status of various structures in the mammary glands from naturally infected dairy cows following slaughter. A total of 94 samples of milk, 184 samples of mammary parenchyma, 168 samples of gland cisterns, and 168 samples of teat cisterns were collected for microbiological examination. Microorganisms were detected in 59.9% of all samples, 67.0% of the milk samples, 70.1% of the mammary parenchymas, 55.9% of the gland cisterns and 48.8% of the teat cistern samples. When all samples were considered, coagulase-negative Staphylococcus were the most prevalent (35.7%) followed by coagulase-positive Staphylococcus (12.2%), Corynebacterium bovis (2.4%), Prototheca sp. (1.9%), and Streptococcus dysgalactiae (1.5%). There was a significantly higher occurrence of microorganisms in the milk and mammary parenchyma compared to the gland cisterns and teat cisterns. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

19.
Four grass plots were sequentially contaminated with goat faeces containing known numbers of unembryonated eggs of predominantly Haemonchus contortus and Trichostrongylus spp. between October 1982 and April 1983. Four other plots were similarly contaminated with sheep faeces between February and May 1987. An additional plot was repeatedly contaminated with sheep faeces from February to April 1987. Populations of free-living stages in faeces and of infective larvae (L3) in the herbage were subsequently monitored until the end of April and June of 1983 and 1987 respectively. During February and May 1987 two control cultures of sheep faeces were incubated in the laboratory at 25°C–30°C and at a constant temperature of 50°C and the free-living development was also monitored. L3 developed very readily in the faeces cultured at 25°C–30°C and in those spread on a grass plot in October, at the end of the wet season, but developed less on the plot contaminated in May at the start of the wet season. Worm eggs in faeces deposited on plots during the hot dry season (December to April) or incubated at 50°C died and disintegrated after 24–48 h exposure to the high environmental temperatures. The results indicate that it is unlikely that gastrointestinal nematodes of sheep and goats can develop or survive on open pasture during the dry season in the Nigerian derived savanna zone.  相似文献   

20.
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