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1.
一株海洋细菌HZBN43的鉴定(英文) 总被引：5，自引：0，他引：5
[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea.[Method]Using 16S rRNA technique,a strain from Yellow Sea was preliminarily identified and analyzed.[Result]One 1 521 bp fragment of 16S rRNA was amplified from the strain HZBN43;homology analysis between the yielded sequence and the 16S rRNA sequences accessed in NCBI from other strains showed that HZBN43 belonged to Bacillus,and shared 99.79% homologue with the known species of Bacillus selenatarsenatis.[Conclusion]The sequence of strain HZBN43 was obtained.However,because of the incomplete sequence,the confidence level is just 46,so other corroborations are still required for grouping HZBN43 into an exact species. 相似文献
2.
NI Guo-rong PAN Xiao-hua TU Guo-quan KUANG Fu-yuan WEI Sai-jin .Key Laboratory of Crop Physiological Ecology Genetics Breeding of Ministry of Education Key Open Laboratory of Double-crop Rice Physiological Ecology Cultivation of Ministry of Agriculture Jiangxi Agricultural University Nanchang .College of Biological Science Engineering 《（《农业科学与技术》）编辑部》2011,(8)
[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate Brail by using cylinder-plate method.Then,it was identified through physiological and biochemical tests,16S rDNA homology analysis as well as some gene-specific sequence analysis.[Result]B221 stain had antimicrobial activity against a variety of rice plant pathogens,and it was identified as Bacillus subtilis.[Conclusion]This study enriches the research on endophyte within D.involucrate Brail,application of Bacillus bio-control,and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens. 相似文献
3.
枯草芽孢杆菌B47高产拮抗物质菌株的紫外诱变选育(英文) 总被引：21，自引：1，他引：20
[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt. 相似文献
4.
《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt. 相似文献
5.
广西北海红树林土壤放线菌的分离与鉴定 总被引：2，自引：0，他引：2
[目的]鉴定广西北海红树林土壤放线菌的物种多样性.[方法]从广西北海红树林土壤中分离、筛选10株典型放线菌菌株,提取基因组DNA,进行16S rDNA PCR扩增与测序,并构建进化树.[结果]通过Blast比对,10株放线菌属于2个属,其中8株为链霉菌属(80%),2株为拟诺卡氏菌属(20%).[结论]广西北海红树林土壤蕴减着种类丰富的放线菌.
Abstract：
[Objective] The aim was to identify the species diversity of Actinomycetes from Mangrove forest in Beihai,Guangxi Province.[Method]10 strains of typical Actinomycetes were isolated from Mangrove forest soil,and the Actinomycetes genomic DNA was successful extracted.16S rDNA was amplified by PCR and sequenced by Sanger dideoxy sequencing method.[Rcsult]All the sequences were blasted in genbank,eight strains belonged to the genus of Streptomyces (80%),and two strains belonged to the genus of Nocardiopsis (20%).[Coacluslon]There are many different Actinomycetes species in Mangrove forest soil samples in Beihai,Guangxi Province. 相似文献
6.
Screening for the Strain Highly Producing Antagonistic Substance from Bacillus subtilis B47 by UV Mutagenesis 总被引：4，自引：0，他引：4
WANG Jing ZHU Jian-hua LIN Wei HUANG Yong-lu YUAN Gao-qing LI Qi-qin Agricultural College of Guangxi University Nanning 《（《农业科学与技术》）编辑部》2008,(4)
[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt. 相似文献
7.
[目的]确定嗜热蛋白酶生产菌DPE7的系统发育地位。[方法]通过PCR方法扩增出嗜热蛋白酶生产菌DPE7的16 S rRNA基因片段,并对其进行了克隆和测序。[结果]对该序列在GenBank中的BLAST结果表明,相似性高于99%的序列中大部分是泥土芽胞杆菌的16 S rRNA基因序列,其中与Geobacillus toebii T1680的16 S rRNA基因序列相似性达99.60%。对菌株DPE7和其他13株泥土芽胞杆菌的16 S rRNA基因序列进行系统发育分析,菌株DPE7与其中的4株泥土芽胞杆菌聚类在一起。[结论]经16 S rRNA基因序列同源性比较和系统发育分析,确定菌株DPE7为泥土芽胞杆菌。 相似文献
8.
[目的]对新疆冰川冰缘地区冻土中筛选出的具有良好产低温淀粉酶能力的菌株进行分子生物学鉴定,分析其酶学特性.[方法]用种子培养液活化菌株提取基因组DNA进行PCR扩增,将扩增成功菌株的PCR产物测序结果通过NCBI数据库比对,对冻土中产低温淀粉酶菌株的16S rDNA序列用MegAlign Fasta软件进行系统发育分析.利用Yoo改良法测低温淀粉酶酶活力.[结果]菌株A-2,A-3,A-4经16S rDNA序列分析获得的序列与数据库中解淀粉芽孢杆菌(Bacillus amyloliquefaciens strain NBRC 15535 16S ribosomal RNA,partial sequence(NR_04145))比对相似率达到99;.该菌株产生的低温淀粉酶的最适作用温度为30℃,最适pH为6.[结论]菌株A-2,A-3,A-4均属解淀粉芽孢杆菌. 相似文献
9.
番茄内生细菌的分离及其促生活性研究(英文) 总被引：5，自引：1，他引：4
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity. 相似文献
10.
番茄内生细菌的分离及其促生活性研究(英文) 总被引：4，自引：0，他引：4
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity. 相似文献
11.
一株产紫杉醇的曼地亚红豆杉内生真菌的分离及鉴定 总被引：1，自引：0，他引：1
[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌.[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物.[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45～50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌.[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种.
Abstract：
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media.[Method]32 strains of endophytic fungi were identified form the inner bark of T.media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp.through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp.M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process. 相似文献
12.
高温纤维素分解菌的分离、筛选及鉴定 总被引：2，自引：0，他引：2
［目的］为研制堆肥高温菌剂和生产高温纤维素酶奠定基础。［方法］从牛粪自然堆肥中分离出高温纤维素分解纤维素菌,并测定其外切纤维素酶活（C1酶）、羧甲基纤维素酶活（CMCase）、FPA酶活（FPase）。［结果］从5种高温纤维素分解菌中,筛选出分解纤维素较强的高温细菌HB4和高温霉菌HM。［结论］高温细菌HB4的C1酶、CMCase、最高,分别为0.016 1 IU/ml、0.923 8 IU/ml;高温霉菌HM的FPA酶活最高,为1.053 5 IU/ml。经初步鉴定HB4为热酸菌属（Acidothermus）,HM为链孢霉菌属（Neurospora）。 相似文献
13.
牛体外发育胚胎特定阶段差异表达基因的研究 总被引：1，自引：0，他引：1
[目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异.[方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比.[结果]该序列与牛核糖体蛋白131基因(ribosomal protein 131,RPL3])具有99%的同源性.采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍.[结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据.
Abstract：
The cattle different stage embryos obtained from in vitro was studied using the technology of single preimplantation embryo mRNA different display:single 8-cell and blastocyst stage embryos were studied using technology of mRNA different display and one different fragment was found.The result suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31.Then to detect the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos by real-time quantitative PCR,the result showed the relative amount of 8 cells was 3.2 times of blastocyst's. 相似文献
14.
依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析.
Abstract：
Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results. 相似文献
15.
[目的]构建抗黄瓜花叶病毒RNAi载体,并将载体转入烟草.[方法]采用RT-PCR方法,扩增黄瓜花叶病毒NS04加工番茄分离物的RNA2基因组的序列选取CMV RAN2基因组中的复制酶片段作为靶序列,构建pBi35SCR2真核表达载体,并对表达载体时行鉴定;通过农杆菌介导的方法将表达载体转入烟草,用PCR的方法检测载体是否转入.[结果]系统进化树分析结果表明,RNA2中编码CMV-2a的序列与中国浙江的DQ412731 分离物有较高核苷本乡酸及氨基酸同源性,分别达到98.0%和96.5%;RCR结果表明,试验成功构建了pBi35SCR2真核表达载体,并成功将表达载体转入烟草[结论]试验获得的转基因烟草可作为后期攻毒试验的材料,并为研究加工番茄抗黄瓜花叶病毒奠定了基础.
Abstract：
[Objective] Aimed to construct RNAi vector resistant to cucumber mosaic virus and transferred this vector into tobacco.[Method]RT-PCR method was used to amplify cucumber mosaic virus NSO4 and process RNA2 gene sequen of tomato isolates.The analysis results of phylogenetic tree demonstrated that the sequence in RNA2 encoded CMV-2a had 98.0% and 96.5% homology with nucleotide and amino acid of DQ412731 isolate of Zhejiang,China.The replicase fragment in CMV RAN2 gene was taken as target sequence to construct pBi35SCR2 eukaryotic expression vector,then the expression vector was identified.Through agrobacterium-mediated method,the expression vector was transferred into tabacco and PCR method was used to check the transfer.The PCR results demonstrated that the experiment had successfully construct eukaryotic expression vector of pBi35SCR2 and the expression vector was successfully transferred into tabacco. [Conclusion] The obtained transgenic tobacco could be used as challenge test material in following experiment and provided foundation for studying processing tomato resist cucumber mosaic virus. 相似文献
16.
底栖鱼类对水田上覆水中磷素动态的扰动效应 总被引：2，自引：0，他引：2
[目的]研究底栖鱼类泥鳅对水田上覆水中磷素动态的扰动效应,探讨生物扰动机制.[方法]基于模拟试验,使用离子色谱法和分光光度法,对比分析上覆水中磷素含量在有/无泥鳅活动时的差异.[结果]扰动组的TP、DTP和PP浓度在试验开始阶段与对照组无显著差异,在试验中、后期显著高于对照(P<0.05).扰动组要的PP/TP高于对照组,扰动组中TP浓度的增加主要是由于PP的增加,扰动组的DIP/DT在试验中、后期显著高于对照(P<0.05).[结论]底栖鱼类对水田上覆水中的磷素产生了扰动作用,增加了水稻生长可利用的的磷素养分.
Abstract：
[Objective] The research aimed to investigate the bioturbation effects of benthic fish Misgurnus anguillicaudatus on phosphorus dynamic in overlying water of paddy field,as well as to explore the bioturbation mechanism.[Method]Based on simulation experiment,the phosphorus contents in overlying water were analyzed comparatively with and without Misgurnus anguillicaudatus by the using of ion chromatography and spectrophotometry. [Result] The concentrations of total phosphorus (TP),dissolved total phosphorus(DTP)and particular phosphorus(PP) in bioturbation group had no significant differences with those in control group in initial stage of experiment,and became significantly higher than control group in middle and late stages of experiment(P<0.05).The PP/TP ratios in bioturbation group were bigger than those in control group,the increase of TP concentration in bioturbation group was mainly due to the increase of PP.The ratios of dissolved inorganic phosphorus(DIP) to DTP (DIP/DTP) were significantly bigger than those in control group in middle and late stages of experiment (P<0.05). [Conclusion] The benthic fish had bioturbation effects on phosphorus in overlying water of paddy field,which increased the available phosphorus for rice growth. 相似文献
17.
提出基因之间传递共表达可作为一个重要因素来连接同一代谢通路中的基因,而同一代谢通路中的功能相似的基因都是高表达相关的.因此可通过求代谢通路下的最短路径,南同在一条最短路径上的与未知基因高表达相关的已知基因来预测末知基因的功能.通过用最短路径算法分析拟南芥代谢通路下的共表达数据对未知基因的功能进行预测,证明了此方法可以预测出拟南芥代谢通路下未知基因的功能,并验证了通过在代谢通路下求最短路径来预测基因功能的方法具有一定的可行性和有效性.
Abstract：
The present paper predicted the function of unknow genes by analyzing the co-expression data of Arabidopsis thaliana from biological pathway based on the shortest-path algorithm.This paper proposed that transitive co-expression among genes can be used as an important attribute to link genes of the same biological pathway.The genes from the same biological pathway with similar functions are strongly correlated in expression.Moreover,the function of unknown genes can be predicted by the known genes where they are strongly correlated in expression lying on the same shortest-path from the biological pathway.Analyzing the Arabidopsis thaliana from the biological pathway,this study showed that this method can reliably reveal function of the unknown Arabidopsis thaliana genes and the approach of predicting gene function by transitiving coexpression in shortest-path is feasible and effective. 相似文献
18.
不同土地利用方式下球囊霉素相关土壤蛋白与有机碳及土壤质地的关系 总被引：4，自引：0，他引：4
[目的]揭示土地利用方式对球囊霉素相关土壤蛋白(GRSP)的影响.[方法]测定了海南岛5种主要土地利用方式下GRSP、土壤有机碳(Soil oaganic carbon)与土壤质地(Soil texture)在0～10、10～20、20～50cm3个土壤层次中的分布情况.[结果]相对于保持较好的次生林,4种不同耕作土壤的GRSP与有机碳含量均有所下降.GRSP在海南岛5种主要土地利用方式土壤中的含量为0.53～4.80 mg/g,占有机碳的百分比为7.9%～23.4%,是碳库的重要组成部分.GRSP占有机碳的比例在不同土地利用方式下差异显著,在土层之间差异不显著,GRSP和有机碳含量在土壤剖面上垂直分布较明显.GRSP与有机碳、砂粒含量呈显著正相关,与粉粒和粘粒呈显著负相关.[结论]砂粒含量在很大程度上决定了CRSP的含量,壤土相对粘土更有利于GRSP的累积.
Abstract：
[Objective] The aim was to reveal the effects of different land use types on soil composition.[Method]GPRS,Soil organic carbon content and soil texture in 3 depths(0-10,10-20,20-50 cm)of 5 main kind of selected land use type were examined in Hainan. [Result] The results showed that GRSP and SOC content of four artificial land use types decreased compared with the natural sacondary foreSt land,the GRSP content of all samples ranged from 0.53-4.80 mg/g,accounting for 7.9%-23.4% of the SOC,which means that GRSP was one important component of SOC pool in soil.The ratio of GRSP to SOC was signiflcantly different among land use types but the depths.GRSP and SOC exhibited obvious vertical distribution pattern.GRSP was significantly positively related to SOC and sand content but negatively related to silt and clay content. [Conclusion] The sand content determined the GRSP content significantly and Joarn was better matrix for GRSP accumulation than clay. 相似文献
19.
以黔江区为研究对象,通过对生态环境影响因子的分析,发现制约黔江区土地利用环境发展的因素主要有区域气候变化、土壤要素、水文环境以及水土流失和土地退化等.并任以上分析的基础上,进一步分析了土地利用变化对生态环境的影响和制约黔江生态环境及区域发展的因素,最后提出了适应山区土地利用与生态环境协凋的对策,对黔江社会经济发展和生态建设具有重要意义.
Abstract：
Accompanying the development of social economy,the land use model of mountainous area,typically eco-weak area,is changing gradually.Here the establishment of eco-friendly land use model in mountainous area,will pioneer the model of sustainable development in that area.Concerning Qianjiang District of Chongqing Municipality,huge change of land use model,mainly embodied in the unceasing increase of construction land and gradual decrease of agricultural use land,has taken place in recent years.To explore the eco-friendly land use model in mountainous area,Qianjiang District was chosen as the study object in the present study.Via analyzing the changes in land use model,we found that related eco-environment restrictive factors mainly regional,climatic change,soil texture,hydrological environment as well as soil erosion and land degradation,etc.And based on these results,we further analyzed the effect of land use change on eco-environment and the factors restricting the maintenance of eco-environment and regional development,finally put forward the counter measures for balancing land use and co-environment in mountainous area.The results will be important for the development of social economy and eco-system construction in Qianjiang District. 相似文献