共查询到20条相似文献，搜索用时 15 毫秒
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Zhang Cai-xia Tian Yi Zhang Li-yi Zong Ze-ran Cong Pei-hua 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(2):329-341
Ring rot disease, caused by the Botryosphaeria berengeriana f. sp. piricola pathogen, is a destructive disease for apple production. To gain further understanding about the defense mechanisms of apple branches against ring rot disease, a comparative proteomic analysis was conducted in our study. We selected two different host responses to B. berengeriana f.sp. piricola infection or challenge, and compared the different proteomes of susceptible and resistant apple branches that had or had not been inoculated with the pathogen. By using 2-DE and MALDI-TOF-TOF MS analysis, 27 differentially expressed proteins were identified in two inoculation assays. According to their function, the proteins were categorized into five classes. In total, according to these two inoculation assays, there were six differentially expressed defense-related proteins identified in the bark of susceptible and resistant hosts, including Mal d1, ASR, and SAMS, which may play key roles for the resistance mechanisms of each host against ring rot disease. We speculated that the only up-regulation of the ASR protein and the dramatic decrease of SAMS in the resistant host may be related to its better disease resistance. In addition, a total of 10 proteins exhibited opposite expression patterns in the bark of susceptible and resistant branches, and they may also be related to the different disease resistances of the two hosts. Due to the complexity of antifungal mechanisms of apple branch hosts against ring rot disease, to obtain more valuable insights about the interaction between the apple host and B. berengeriana f. sp. piricola pathogen, many further investigations will be conducted. 相似文献
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A putative serine protease with a potential role in the plant biotic and abiotic stress response was purified from wheat leaf apoplastic fluid and partially characterized. Following two-dimensional electrophoresis a protein of Mr = 75 k and a pI of 4.2 to 4.5 was observed. This protein displayed in-gel protease activity and was specifically inhibited by phenylmethanesulfonyl fluoride and partially inhibited by Ca2+ and Zn2+, but not by E-64 or leupeptin. An internal tryptic fragment of 13 amino acids was identified by MALDI QqTOF MS/MS, and this peptide showed a high level of homology (85–100 % identity) to a highly conserved region of known plant subtilisin-like proteases. We demonstrated that the protease activity increased until a late stage of wheat leaf development and increased in response to heat shock. In both cases Rubisco large subunit was degraded with time. Protease activity was also increased during biotic stress. Leaves challenged with leaf rust (Puccinia triticina), showed an approximately three fold increase in protease activity during an incompatible interaction, compared to activity in mock-inoculated leaves and to leaves in a compatible leaf rust interaction. These results suggest that the expression of this serine protease could be involved in the defense response against both abiotic and biotic stresses. 相似文献
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Geleta Dugassa Barka Eveline Teixeira Caixeta Robson Ferreira de Almeida Samuel Mazzinghy Alvarenga Laércio Zambolim 《European journal of plant pathology / European Foundation for Plant Pathology》2017,149(3):543-561
Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (non-specific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed. 相似文献
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Nurul Shamsinah Mohd Suhaimi Rozeita Laboh Noni Ajam Kwai Lin Thong 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(1):13-26
A blood disease pathogenic strain, Ralstonia syzygii subspecies celebesensis was used to study the possible association of biofilm-forming bacteria with the development and severity of blood disease in banana plants. Therefore, the objective of this study was to determine the effects of mono-culture and co-culture inoculation of isolated biofilm-forming bacteria with the blood disease pathogen in banana pseudostems in glasshouse conditions. Putative biofilm-forming bacteria were isolated from an infected banana plant and were further identified using 16SrRNA sequencing. Four isolates, identified as Enterobacter hormaechei, Enterobacter cloacae, Kosakonia radicincitans and Klebsiella pneumoniae, were inoculated as a mono- and co-culture with R. syzygii subsp. celebesensis into 2 months old banana plants. The observation after the 8 weeks of post inoculation showed that plants which were co-inoculated with the pathogen and K. radicincitans, a biofilm-forming bacterium, were the most susceptible towards the infection. In contrast, plants under two treatments (which were co-inoculated with the pathogen and E. cloacae and the pathogen with E. hormaechei) were less susceptible towards the infection. This study revealed the antagonistic effects of two biofilm-forming strains which reduced the severity of infection caused by the pathogenic agent. Scanning electron micrographs of the cross section of plant rhizomes indicated the dissimilarity of adhesion and host colonization conditions of the pathogen in each infected plant from different treatments. 相似文献
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Katarzyna Szajko Danuta Strzelczyk-Żyta Waldemar Marczewski 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(2):375-385
The Ny-1 and Ry-fsto genes of potato (Solanum tuberosum L.) confer hypersensitive response (HR) and extreme resistance (ER), respectively, to Potato virus Y (PVY). ER-type resistance was also observed in potato plants with both alleles (Ny-1 and Ry-fsto). Using two-dimensional electrophoresis (2-DE), quantitative differences between PVY-infected and non-infected control plants were found for 35, 32 and 15 protein spots identified in leaves of potato cultivar Rywal (possessing Ny-1), and potato tetraploid clones PW 363 (with Ry-fsto) and PB 08–137 (with Ny-1 + Ry-fsto), respectively. We recognized 29, 12 and 21 PVY-induced protein spots involved in qualitative changes in Rywal, PW 363 and PB 08–137 plants, respectively, which were processed and analysed using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system. A database search indicated that these 62 proteins belong to various functional categories. Their expression was genotype-specific. In the case of cultivar Rywal with HR-mediated resistance, proteins involved in photosynthesis and primary metabolism were the most abundant. For PW 363 and PB 08–137, both with ER–mediated resistance, stress-responsive proteins were the most numerous. Only two proteins – glutamate–glyoxylate aminotransferase 2 (GGAT2) and monodehydroascorbate reductase 5 (MDHAR5) – were identified in all three genotypes. These two proteins are components of the reactive oxygen species (ROS) defence mechanism against pathogens in plants. The present study showed that the differences in PVY-induced proteins in the leaves of Ny-1, Ry-fsto and Ny-1 + R-fsto genotypes do not correspond to the type of gene conferring the resistance or to the observed phenotype. 相似文献
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Anita Haegi Simona De Felice Michele Scotton Laura Luongo Alessandra Belisario 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(4):787-801
Bread wheat (BW) and durum wheat (DW) are both strongly affected by Septoria tritici blotch caused by the hemibiotrophic fungus Zymoseptoria tritici. However, only the BW-Z. tritici pathosystem has been well studied so far. Here, we compared compatible interactions between Z. tritici and both BW and DW species at the cytological, biochemical and molecular levels. Fungal infection process investigations showed close spore germination and leaf penetration features in both interactions, although differences in the patterns of these events were observed. During the necrotrophic phase, disease severity and sporulation levels were associated in both interactions with increases of the two cell-wall degrading enzyme activities endo-β-1,4-xylanase and endo-β-1,3-glucanase as well as protease. An analysis of plant defense responses during the first five days post inoculation revealed inductions of GLUC, Chi4, POX and PAL and a repression of LOX gene expressions in both wheat species, although differences in kinetics and levels of induction or repression were observed. In addition, peroxidase, catalase, glucanase, phenylalanine ammonia-lyase and lipoxygenase activities were induced in both wheat species, while only weak accumulations of hydrogen peroxide and polyphenols were detected at the fungal penetration sites. Our study revealed overall a similarity in Z. tritici infection process and triggered wheat defense pathways on both pathosystems. 相似文献
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Ilze Beukes Lindy Joy Rose Gerhardus Johannes van Coller Altus Viljoen 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(4):893-910
Fungal species comprising the Fusarium graminearum species complex (FGSC) may cause disease in maize and wheat. Host preference within the FGSC has been suggested, in particular F. boothii towards maize ears. Therefore, the disease development and mycotoxin production of five FGSC species in maize and wheat grain was determined. Eighteen isolates representing F. acaciae-mearnsii, F. boothii, F. cortaderiae, F. graminearum and F. meridionale were used. Each isolate was inoculated on maize ears and wheat heads to determine host preferences. Disease severity and disease incidence was measured for maize and wheat, respectively. Fungal colonisation and mycotoxins, deoxynivalenol (DON), nivalenol and zearalenone, was also quantified. Isolates differed significantly (P < 0.05) in their ability to produce symptoms on maize ears, however, no significant differences between FGSC species were determined. Similarly, significant differences (P < 0.05) between isolates but not between FGSC species in disease incidence on wheat were determined. The isolates also differed significantly (P < 0.05) in their ability to colonise maize and wheat grain. No significant differences in fungal colonisation, among the five FGSC species, were determined in field grown maize. However, under greenhouse conditions, F. boothii was the most successful coloniser of maize grain (P < 0.05). In wheat, F. graminearum colonised the grain more successfully and produced significantly more (P < 0.05) DON than the other species. Fusarium boothii isolates were the best colonisers and mycotoxin producers in maize, and F. graminearum isolates in wheat. The selective advantage of F. boothii to cause disease on maize was supported in this study. 相似文献
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The Rhizoctonia solani species consists of multinucleate isolates that belong to anastomosis groups AG1–AG3 and differ in virulence and host affinity. R. cerealis is a binucleate species of anastomosis group AG-D which causes sharp eyespot, a common plant disease in Poland. Rhizoctonia spp. is a ubiquitous soil pathogen that poses a significant threat for global crop production due to the absence of effective crop protection products. The aim of this study was to determine the virulence of R. solani and R. cerealis isolates towards Beta vulgaris, Zea mays, Triticum spelta and T. aestivum seedlings, to confirm the presence of endopolygalacturonase genes pg1 and pg5 in the genomes of the tested isolates and to evaluate the tested isolates’ sensitivity to triazole, strobilurin, imidazole and carboxamide fungicides. All tested isolates infected B. vulgaris seedlings. but none of them were virulent against Z. mays plants. R. solani isolates AG4 PL and AG2-2IIIB PL were characterized by the highest virulence (average infestation score of 2.37 and 2.53 points on a scale of 0–3 points) against sugar beet seedlings. The prevalence of infections caused by most of the analysed isolates (in particular R. solani AG4 J—11.8, and R. cerealis RC2—0.78) was higher in spelt than in bread wheat. The virulence of the analysed isolates was not correlated with the presence of pg1 and pg5 genes. The efficacy of the tested fungicides in controlling Rhizoctonia spp. infections was estimated at 100% (propiconazole + cyproconazole), 98.8% (penthiopyrad), 95.4% (tebuconazole) and 78.3% (azoxystrobin). 相似文献
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Bacterial blight is a highly devastating disease caused by Xanthomonas axonopodis pv. punicae, recording 60 to 80 percent yield-loss of pomegranate in India. In the present investigation, a total of 209 genotypes including 105 exotic types from USDA, 66 wild types and 38 cultivated types from India were screened and categorized into fifteen clusters using cluster and principal component analysis. Genotypes of cluster 15, viz. 108 B and 99 A from USDA and 318734, Daru-18 and IIHR-30 from India, were found to be resistant to bacterial blight while genotypes of cluster 9 were highly susceptible. Two genotypes, each from cluster 15 (318734) and 9 (Ruby), were compared for biochemical and histological parameters to understand the defense mechanism. Significantly, higher accumulation of defense related metabolites, viz. total phenol, flavonoid and antioxidant contents, were observed in resistant genotype (318734). Fewer numbers of stomatal pores that served as portals of entry for plant pathogens were recorded in this genotype. Resistance observed in genotype 318734 might be due to an incompatible interaction between host and pathogen compared to other genotypes. This is the first report of putative resistance sources in pomegranate against Xanthomonas axonopodis pv. punicae. 相似文献
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The soilborne pathogen Rosellinia necatrix causes white root rot, a serious disease of various trees, and is extremely difficult to control. In this study, using one-dimensional electrophoresis coupled with nanoliquid chromatography-electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS), we identified 696 proteins from R. necatrix mycelium (KACC 40445) grown in liquid culture. In addition, 573 proteins were assigned to at least one gene ontology term including 26 functional groups. Most were related to catalytic activity in the molecular function category. This proteomic data set advances understanding of R. necatrix biology and will inform further investigations to manage white root rot using novel strategies. 相似文献
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J. W. Woodhall M. J. Brown K. Perkins E. Somoza Valdeolmillos N. Boonham R. V. Ray 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(2):237-245
Rhizoctonia cerealis causes sharp eyespot in cereals and the pathogen survives as mycelia or sclerotia in soil. Real-time Polymerase Chain Reaction (qPCR) assays based on TaqMan chemistry are highly suitable for use on DNA extracted from soil. We report here the first qPCR assay for R. cerealis using TaqMan primers and a probe based on a unique Sequence Characterised Amplified Region (SCAR). The assay is highly specific and did not amplify DNA from a range of other binucleate Rhizoctonia species or isolates of anastomosis groups of Rhizoctonia solani. The high sensitivity of the assay was demonstrated in soils using a bulk DNA extraction method where 200 μg sclerotia in 50 g of soil were detected. DNA of the pathogen could also be amplified from asymptomatic wheat plants. Using the assay on soil samples from fields under different crop rotations, R. cerealis was most frequently detected in soils where wheat was grown or soil under pasture. It was detected least frequently in fields where potatoes were grown. This study demonstrates that assays derived from SCAR sequences can produce specific and sensitive qPCR assays. 相似文献
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M. Otto Y. Petersen J. Roux J. Wright T. A. Coutinho 《European journal of plant pathology / European Foundation for Plant Pathology》2018,150(2):427-437
Laboratory and nursery experiments were conducted to identify the causal agent of a needle blight of Pinus wallichiana, a species native to the Western Himalayas. The pathogen was identified as Myrothecium verrucaria, on the basis of morphological, cultural and molecular characterization. BLAST analysis of ITS sequences of the pathogen revealed maximum sequence identity of 99% with M. verrucaria. The sequence is the first of this fungus from P. wallichiana. Phylogenetic analysis grouped all M. verrucaria isolates in a single clade; M. roridum and M. inundatum clustered in separate clades. The pathogen grew optimally at 25 ± 1 °C on oat meal agar, pH 5.5. Inoculation experiments with M. verrucaria demonstrated pathogenicity on Pinus halepensis, Cedrus deodara and Cryptomeria japonica, in addition to Pinus wallichiana. 相似文献
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Stripe rust is considered as the current major rust disease affecting winter cereal production across the world. A quick, reliable PCR-based marker was developed here to detect, identify and rapidly monitor Puccinia striiformis f. sp. tritici (Pst) in wheat-growing areas. Three respective sets of primers, designed from β-tubulin, squalene monooxygenase and ketopantoate reductase genes selected from the full genome of Puccinia striiformis f. sp. tritici, amplified sequences of 239, 358 and 1518 bp, respectively, in Pst pathotypes. A fragment of 1518 bp unique to Pst pathotypes was amplified using primer set PstKeto F1_30/Pst KetoR1_1547 and distinguished the pathogen clearly from different Puccinia spp. and other fungal pathogens. The detection limit of the marker (KetoPstRA1500, accession no. KU240073) by conventional PCR assay was 10 pg. This marker could detect the pathogen in the host before symptom expression. The sensitivity and utility of the marker were further enhanced in a qPCR-based assay that was developed with a newly designed primer set PstKeto F1_1246/Pst KetoR1_1547, which amplified a product of 302 bp and detected as little as 10 fg of DNA. This PCR/qPCR based marker is suitable for studying cultivar resistance, which requires accurate quantification of the pathogen in diseased host tissue. 相似文献
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In this investigation, leaf extracts of Ocimum basilicum and Mangifira indica were used as reducing agents for biosynthesis of silver nanoparticles (AgNPs). The biosynthesized AgNPs were authorized by UV-vis spectrophotometry and X?ray diffraction (XRD) analysis. AgNPs were obtained after 5?min of reaction at 80oC. The formation of AgNPs was confirmed by the presence of absorption peaks at 439?nm using extract from O. basilicum and at 442.5?nm from M. indica. X?ray spectra showed strong peaks for the crystalline Ag. Shape and size of the biosynthesized AgNPs were studied using high resolution transmission electron microscope (HR-TEM). Size of the produced AgNPs was found to be 9–35?nm. Effect of the synthesized nanosilver was then investigated on some biochemical attributes of wheat plant (Triticum aestivum cultivar saka 92). The growth parameters such as shoot lengths, fresh and dry weight of shoot, chlorophyll, carbohydrate and protein contents in shoot of wheat plant were investigated. Application of AgNPs synthesized from Ocimum basilicum and from Mangifira indica at the concentrations of 20,40?ppm showed an increase in shoot length, fresh and dry weight of shoot, chlorophyll, total carbohydrate and protein content in shoot of wheat plants, beyond these concentrations an inhibitory effects were shown. 相似文献