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1.
AIM: To examine whether tolerogenic dendritic cells (DC) loaded with heat shock protein 60 (HSP60) could restore endothelial function in hypercholesterolemic apolipoprotein E (apoE)-null mice.METHODS: Bone marrow derived DC of the mice was loaded with HSP60 and co-cultured with rapamycin to generate tolerogenic DC.The tolerogenic DC, DC loaded only with HSP60 (DChsp) and saline were injected into the apoE-null mice at 6 weeks of age for two times at a one-week interval.C57BL/6 mice at the same age were taken as normal control two weeks after the last injection.Aorta was harvested for ex vivo vascular ring tension test.Immune parameters were also analyzed in vitro and in vivo.RESULTS: Compared with the non loaded DC, HSP60 pulsed DC expressed higher levels of CD86, and stimulated T lymphocytes to proliferation significantly, while the tolerogenic DC expressed lower levels of CD86, and inhibited T lymphocytes to proliferation.After immunization with different injection, Ach-induced relaxation was reduced significantly in DChsp group compared with saline group (P<0.01).Treatment of mice with tolerogenic DC restored endothelium-dependent dilation in a dose-dependent manner (P<0.01).The improvement in endothelial function was associated with a reduction in T cell response to HSP60.CONCLUSION: Our results indicate a rapid improvement in endothelial function with HSP60 tolerogenic DC immunization, and suggest that this immune therapy has significant vasculoprotective effects.  相似文献   

2.
AIM:To investigate the effects of invariant natural killer T-cells (iNKT cells) from ovalbumin (OVA)-induced asthmatic mice combined with OVA on the phenotypic and functional characteristics of bone marrow-derived dendritic cells (BMDCs) in vitro. METHODS:The BMDCs from wild-type (WT) BLAB/c mice were co-cultured with purified iNKT cells from WT mice immunized and challenged with OVA in the presence of 100 mg/L OVA (iNKT cells plus OVA group) or PBS (iNKT cells plus PBS group) for 20 h, and were also cultured with 50 mg/L LPS (LPS group), 100 mg/L OVA (OVA group) or PBS (PBS group) for 20 h. The expression of MHC-Ⅱ, CD40, CD86, and CD80 on the BMDCs was measured by flow cytometric analysis, and the levels of interleukin-12 (IL-12) p70, IL-6, tumor necrosis factor-α (TNF-α) and IL-10 in the culture supernatant were measured by ELISA. Splenic CD4+ T cells from DO11.10 transgenic mice were co-cultured for 48 h with the above mentioned BMDCs, and then the concentrations of IL-4 and interferon-γ (IFN-γ) in culture supernatants were measured by ELISA. RESULTS:The expression of MHC-Ⅱ, CD80, CD86 and CD40, and releases of proinflammatory cytokines by BMDCs in iNKT cells plus OVA group were comparable to those in the LPS group (P>0.05), but significantly higher than those in iNKT cells plus PBS group, OVA group, and PBS group (P<0.05 or P<0.01). The concentration of IL-4 in culture supernatants from BMDCs in iNKT cells plus OVA group co-cultured with DO11.10 CD4+ T cells was similar to that in LPS group (P>0.05), but markedly higher than that in iNKT cells plus PBS group, OVA group, and PBS group (P<0.01). CONCLUSION:Bone marrow-derived dendritic cells undergo immunogenic maturation upon interaction with iNKT cells in the presence of OVA.  相似文献   

3.
AIM: To investigate NIH3T3 cell proliferation and cell cycle in the condition of hypoxia or low nutrition and its response to bFGF, and to explore the pathophysiological changes of fibroblast under hypoxic or low nutrional conditions.METHODS: The cells were placed in anaerobic workstation where the mixture gas was given to simulate hypoxic environment. Partial oxygen pressure (PO2) of medium was controlled in 27 mmHg, 44 mmHg and 175mmHg. NIH3T3 cells were cultured with low nutritional medium contained new bovine serum (NCS) less than 10% to simulate low nutritional environment. MTT assay was used for observing cell activity and flow cytometry for cell cycle analysis.RESULTS: Under 44 mmHg PO2, no obvious difference was shown between hypoxia group and normal group. Under 27 mmHg PO2, the proliferation activity of NIH3T3 cells was significantly lower than that in normal group (P<0.01), as well as the cell numbers in G0-G1 phase increased (P<0.05), S phase decreased (P<0.01). bFGF had no effect on cell proliferation. In 0.5% NCS medium, the NIH3T3 cell proliferation speed decreased (P<0.01) and cell cycle was arrested at G0-G1 (P<0.01). The proliferation speed was improved by bFGF (P<0.01).CONCLUSION: In lower PO2 or lower nutrinal condition, fibroblast proliferation activity is inhibited by cell cycle arrest in G0/G1 phase. However the decreasing proliferation in low nutritional medium could be improved by external bFGF.  相似文献   

4.
AIM:To study the anti-tumor effect and mechanism of fusion vaccine on DCs and C6 glioma cells. METHODS:PEG was used to fuse DCs with C6 glioma cells. Immunofluorescence with GFAP-FITC was used to identify the DC/C6 fusion cells. Rat brain glioma models were made by stereotactic technique. After 5 days of inoculation of C6, 107 fusion cells were injected through tail vein in group A. The same number of DCs and the same volume of PBS were used in group B and group C. The survival time of rats in these three groups was analyzed by Log-rank survival analysis. Tumor samples were checked by HE staining and immunohistochemical staining with CD8Mcab. RESULTS:Positive result of GFAP-FITC immunofluorescence was observed in DC/C6 fusion cells. The Log-rank survival analysis showed that statistically significant difference in group A was observed compared to that in group B and group C (P<0.01). Tumor sample stained with HE showed that many inflammatory cells infiltrated in tumor tissues. The result of immunohistochemical staining with CD8Mcab was positive. CONCLUSION:DC/C6 fusion cells had the ability of antigen presentation and activating T lymphocytes effectively. CD8+T lymphocytes play an important role in antitumor immunity.  相似文献   

5.
AIM:To study the effect of adoptive transfer of CD4+ T cells with microRNA-7 (miR-7) knockdown (KD) on mouse acute liver injury model and to investigate its significance. METHODS:CD4+ CD62L+ T cells were purified from the spleen of normal wild-type (WT) mice and miR-7KD mice by magnetic bead sorting, and were stained with CFSE. These 2×106 CFSE-labeling cells were injected into normal mice via tail vein, and then the mouse acute liver injury model was induced by intraperitoneal injection of 30 mg/kg concanavalin A. After 72 h, the appearance, weight and weight index of the liver were investigated. The pathological change of the liver tissues was observed by HE staining. Real-time PCR was used to examine the mRNA expression of Bax and P53. The expression levels of CD62L, interleukin-4 (IL-4) and interferon-γ (IFN-γ) in the CD4+ T cells were analyzed by flow cytometry. RESULTS:We found that the liver tissue became lighter, and the weight (P<0.01) and weight index (P<0.05) were changed significantly in miR-7KD mice compared with control group. Moreover, HE staining showed that the liver cell damage was increased in the liver of miR-7KD mice. Meanwhile, the expression levels of Bax and P53 were significantly increased in miR-7KD group (P<0.05). The percentage of CD62L in CD4+ T cells was significantly decreased (P<0.01) in miR-7KD mice, with high expression of IFN-γ (P<0.05) and low expression of IL-4 (P<0.01) in CD4+T cells. CONCLUSION:These findings suggest that miR-7 knockdown significantly promotes the pathology of CD4+ T cell-mediated acute liver injury, which provides a preliminary experimental basis for further exploration on the mechanism of acute liver injury occurrence.  相似文献   

6.
AIM:To investigate the effects of goat placenta immunoregulating factor (GPIF) on the expression of costimulatory molecules lineaged T cells in BALB/c mice. METHODS:Animal model for immunodeficiency made from BALB/c mice with whole-body irradiation by 5 Gy 60Coγ-ray was applied for research. The immunosuppressive mice were injected with GPIF for seven days continuously. FACS was applied to analyze the rate of CD28+, CD152+, CD4+CD28+, CD8+CD28+, CD4+CD152+ and CD8+CD152+ cells in splenic lymphocytes and ELISA method was employed to measure the amount of IL-2 and IFN-γ in serum of mice. RESULTS:GPIF increased the percentage of CD28+, CD4+CD28+ and CD8+CD28+ cells (P<0.05, P<0.01), and decreased the percentage of CD152+ (P<0.05, P<0.01), CD4+CD152+ cells (P<0.05, P<0.01) in splenic lymphocytes of immunosuppressive mice significantly. GPIF increased the content of IL-2 and IFN-γ in serum of mice simultaneously (P<0.01). CONCLUSION:Immuno-enhancing effect of GPIF facilitates the costimulation of CD28 pathway, which can activate T cells and accelerate the course of renewing T cell activity. The function of GPIF may have close relationship with an immune network formed by the secretion of IL-2 and IFN-γ and the expression of CD28 and CD152.  相似文献   

7.
AIM: To study the effects of dexamethasone (DXM) on intracellular expression of TH1/TH2 cytokines and the mechanism of that during the development of asthma. METHODS: Eighteen BALB/c mice were divided into 3 groups at random: control group, asthma group, and DXM treated group, with 6 mice in each. The expressions of T-box expressed in T cells (T-bet) and GATA-binding protein-3 (GATA-3) in lung tissue were detected by Western blotting. The expressions of intracellular cytokines interleukin-4 and interferon-γ in CD4+ T cell were measured by flowcytometry.RESULTS: The results of flow cytometry indicated that the ratio of intracellular cytokines IL-4/IFN-γ in CD4+ T cells in asthma group was much higher than that in control group (P<0.01), the ratio of intracellular IL-4/IFN-γ in T cells in DXM group was lower than that in asthma group significantly (P<0.01). The expression of T-bet in lung tissue in asthma group was lower than that in control group significantly (P<0.01), while GATA-3 was higher than that in control group significantly (P<0.01). The expressions of T-bet and GATA-3 in DXM group were much lower than those in asthma group (P<0.01), but the decreased degree of GATA-3 was more than that of T-bet. CONCLUSION: With pathological process of asthma, to reverse the ratio of IL-4/IFN-γ in CD4+ T cell by regulating T-bet and GATA-3 expression can improve the inflammatory reaction and may be one of the mechanisms of DXM in treating asthma.  相似文献   

8.
AIM:To investigate the effects of glucocorticoid on the regulation of microRNA-155 (miRNA-155) expression in the CD4+ T cells of asthmatic mice. METHODS:The ovalbumin (OVA)-induced asthma mouse model was established and the mice were treated with glucocorticoid. The effects of glucocorticoid on the pulmpnary histopathological changes, the expression of miRNA-155 in the lung tissues and CD4+T cells, and the levels of cytokines in the bronchoal-veolar lavage fluid (BALF) were evaluated. RESULTS:The results of RT-qPCR showed that the expressions of miRNA-155 in the lung tissues and CD4+T cells from the spleen of asthmatic mice were significantly increased, and the level of miRNA-155 in the CD4+T cells was significantly increased with the increase in the allergen exposure time (P<0.01). HE and PAS staining showed that OVA significantly increased inflammatory cell infiltration as compared with control group, and the peribronchial and perivascular inflammation and mucus secretion of proliferative goblet cells were significantly reduced after glucocorticoid treatment. Glucocorticoid treatment inhibited the increase in the proportion of CD4+ CD8- cells in the spleen and decreased the accumulation of CD4+ T cells in the lung tissues of asthmatic mice (P<0.01). After glucocorticoid treatment, the levels of interleukin-4 (IL-4), IL-5 and IL-13 in BALF were decreased, while the level of interferon-γ was increased significantly (P<0.01). CONCLUSION:Glucocorticoid reduces the accumulation of CD4+ T cells and inhibits the expression of miRNA-155 in the lung tissues and spleen CD4+ T cells of asthmatic mice.  相似文献   

9.
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10.
AIM: To study the roles of bone marrow-derived dendritic cells from donor mouse treated with 17β-estradiol (E2) in immune tolerance induction in skin allograft. METHODS: Bone marrow-derived dendritic cells from C57 mouse as donor were cultured respectively treated with E2 (E2 group). BALB/c mouse as recipient received respectively one injection of dendritic cells of E2 group, mature dendritic cell group and immature dendritic cell group intravenously. Skin transplantation was performed in the absence of immunosupression after 7 d. Mice that received PBS were served as control. The time of skin survival was observed after transplantation. Flow cytometry was used to analyze the percentage of CD4+CD25+ T cells in peripheral blood respectively before and after transplantation. RESULTS: Compared with immature dendritic cells and control group, the time of skin survival in E2 group was significantly longer (P<0.01), especially, the time of skin survival still prolonged 10.6 d after skin rejection in immature dendritic group. The percentage of CD4+CD25+ regulatory T cells in E2 group was significantly higher than that in immature dendritic cell group and control group (P<0.01). CONCLUSION: In skin allograft model, dendritic cells treated with E2 prolong the allograft survival time.  相似文献   

11.
AIM: To study the immunosuppressive effects of early apoptotic T lymphocytes.METHODS: Early apoptotic spleen T cells were induced by ultraviolet irradiation for 5 min.After irradiation,spleen T cells were incubated at 37 ℃ with 5% CO2 for 2 h and thus early apoptotic T lymphocytes were obtained.Three to four freeze thaw cycles resulted in disruption of the spleen T cells into fragments.imdendribic cells(DCs) were prepared from red cells and T cells depleted bone marrow cells.The imDCs were divided into five groups: group A: necrotic spleen T cells were added to imDCs;group B: early apoptotic spleen T cells were added to imDCs;group C: supernatants from early apoptotic spleen T cells alone with necrotic spleen T cells were added to imDCs;group D: TGFβ1 neutralizing antibody along with early apoptotic T lymphocytes were added to imDCs;group E: immature dendridic cells culture in RPMI-1640 for 5 days were used as negative control.Flow cytometry was employed to analyze the expression of MHCⅡ,CD40,CD80 and CD86 on DCs in each group.ELISA was employed to assay the IL-12 p70 produced by DCs in different groups.The amounts of TGFβ1 released by early apoptotic T lymphocytes were also determined by ELISA.T cells proliferation assay was employed to study DCs T cells stimulatory capacity.RESULTS: The DCs expressed high level of MHCⅡ,CD40,CD80 and CD86 when exposed to necrotic cells while early apoptotic cells did not.The supernatants from early apoptotic spleen T cells suppressed the expression of MHCⅡ,CD40,CD80 and CD86 on DCs exposed to necrotic spleen T cells.When TGFβ1 neutralizing antibody along with early apoptotic spleen T were added to imDCs,the expression of MHCⅡ,CD40,CD80 and CD86 was increased significantly.The necrotic spleen T cells increased IL-12 p70 production by DCs,while apoptotic spleen T cells at early stage did not (P<0.01,group B vs group A or B;P>0.05,group B vs group E).Only the DCs that exposed to necrotic spleen T cells gained significant T lymphocytes stimulatory capacity,while DCs exposed to apoptotic cells at early stage did not.The amounts of TGFβ1 released by early apoptotic spleen T cells were much higher than those released by viable spleen T cells.CONCLUSION: Apoptotic spleen T cells at early stage have the capacity to induce the generation of tolerogenic DCs.  相似文献   

12.
WU Qiong  JING Liang 《园艺学报》2007,23(11):2096-2099
AIM: To observe the effect of heat shock protein 70(HSP70) expression induced by glutamine on Escherichia coli lipopolysaccharides(LPS)-induced vascular hyporeactivity in rats.METHODS: Twenty four healthy male Sprague-Dawley rats were randomly divided into: the control group (n=8);LPS shock group (n=8);glutamine(Gln) treated group (Gln 0.75 g·kg-1 iv,n=8).6 h after LPS shock,phenylephrine (PE,0.5-2.5 μg·kg-1 ) was applied intravenously to all groups and the percentage increase in mean arterial pressure(MAP) was detected,respectively.The concentration-response curves of aorta rings were obtained by cumulative addition of phenylephrine (PE),and PE Emax,EC50 were calculated.The blood concentration of malondialdehyde (MDA),TNF-α and IL-6 were assayed in all groups 30 min and 360 min after LPS shock,respectively.The expressions of HSP70 from heart and aorta were also assayed after 6 h LPS shock.RESULTS: The MAP level induced by PE significantly decreased by 51.4% in LPS shock group compared with the control (P<0.05).However,PE induced MAP level increased by 17.5% in Gln group compared with LPS shock group (P<0.05).Emax and EC50 to PE were significant reduced in LPS shock group compared with control group (P<0.05),but significantly improved in Gln group (P<0.05).The expressions of HSP70 from heart and aorta were much higher in Gln group than those in LPS shock group (P<0.05).The blood concentrations of TNF-α,IL-6 and MDA were much lower in Gln group than those in LPS shock group.CONCLUSION: Glutamine effectively improves α-adrenergic receptor-mediated vascular reactivity through inducing the expression of HSP 70,reducing inflammatory cytokine release and peroxide biosynthesis in LPS shock.These results suggest that glutamine have potential beneficial therapeutic effect for septic shock patients.  相似文献   

13.
无 《园艺学报》2012,39(1):142-146
自2008年11月在中国农业科学院郑州果树研究所成功召开园艺植物染色体倍性操作与遗传改良研讨会以来,我国科研人员在该领域的研究取得了可喜的成绩。为进一步总结近几年来在该领域的研究进展,促进园艺植物倍性育种研究,同时为该领域的专家、学者和同仁们提供良好的交流平台。中国园艺学会定于2012年4月中旬在重庆召开园艺植物染色体倍性操作与遗传改良学术研讨会。欢迎从事该研究领域及相关研究工作的人员参加。  相似文献   

14.
AIM: To explore the effects of romidepsin (FK228), a novel histone deacetylase inhibitor, on the effector and regulatory T cells in vitro.METHODS: As the reactive cells, lymphocytes, CD4+ T cells and CD8+ T cells were labelled with CFSE, and stimulated with anti-CD3 and anti-CD28 mAbs in the presence and absence of different levels of romidepsin (experimental group and positive control group), or PBS (placebo group).After 72 h, the proliferation of the cells was detected in different groups. The lymphocytes were stimulated with anti-CD3 and anti-CD28 mAbs in the presence and absence of different levels of romidepsin (experimental group and positive control group),or PBS (placebo group). After 72 h, the percentage of CD4+ Foxp3+ T cells and the levels of related cytokines were detected in different groups. RESULTS: The proliferation of CFSE-labelled lymphocytes, CD4+ T cells and CD8+ T cells triggered by anti-CD3 and anti-CD28 mAbs all were inhibited when cultured with romidepsin at concentrations of 1 μmol/L, 3 μmol/L and 5 μmol/L in a dose-dependent manner (P<0.05). Compared with placebo group, in the presence of anti-CD3 and anti-CD28 mAbs, 1 μmol/L romidepsin did not increase the percentage of CD4+ Foxp3+ T cells (P>0.05). When cultured with romidepsin at concentrations of 3 μmol/L and 5 μmol/L, the percentage of CD4+ Foxp3+ T cells was enhanced markedly (P<0.05). The levels of IL-10 and TNF-α in the supernatant were markedly increased in positive control group and 3 experimental groups (P<0.05), and the levels of cytokines in different experimental groups were gradually decreased with the elevation of FK228 concentration (P<0.05). The level of TGF-β was slightly increased in positive control group with no significant difference compared with placebo group (P>0.05). With the increase in the concentration of FK228 in different experimental groups, the TGF-β level was increased in a dose-dependent manner and there were significant differences in the 3 experimental groups. Meanwhile, significant differences existed between experimental groups and placebo group and between experimental groups and positive control group (P<0.05). CONCLUSION: Romidepsin inhibits the proliferation of CD4+ and CD8+ effector T cells and increases the percentage of CD4+ Foxp3+ regulatory T cells. It may be related to the increased level of TGF-β, but independent of IL-10.  相似文献   

15.
AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.  相似文献   

16.
AIM: To establish HCC Hu-PBL-SCID(severe combined immune deficiency) chimeric model,and to observe the antitumor effect of mRNA-dendritic cell vaccine.METHODS: Hu-PBL-SCID chimeric model was established by intraperitoneal injection of human peripheral blood lymphocytes.Human IgG in mouse serum was detected by ELISA to identify the model.After the model was established,the mice were divided into four groups,and were inoculated with mRNA DC vaccine,anti CD4+,CD8++mRNA DC,naive DC,and PBS,respectively.Then the animals were injected subcutaneously with 2×106 HepG-2 cells.Tumorigenecity,latent period,and tumor volume were observed,and antitumor efficacy of CTL was measured.RESULTS: The concentration of human IgG in mouse serum in Hu-PBL- SCID model was detected,indicating that the Hu-PBL-SCID model was established successfully.No significant difference of tumorigenecity among the four groups was observed.However,tumors in mRNA DC vaccine group grew slowly,tumor volumes were significantly smaller than those in anti CD4+,CD8++mRNA DC,PBS and naive DC groups.The spleen cells in mRNA DC vaccine group specifically killed the HepG-2 cells but not SGC-7901 cells.CONCLUSION: mRNA DC vaccine shows anti-tumor immune response in vivo by inducing CD4+,CD8+T lymphocyte immune responses.  相似文献   

17.
AIM: To investigate the effects of AT-2-inactivated HIV-1 particles on human CD4+T cell activation and cytokine secretion in whole blood (WB) in vitro. METHODS: HIV-1ⅢB particles were inactivated by AT-2 chemical and the concentration of p24 antigen was determined by p24 ELISA. AT-2-inactivated HIV-1ⅢB particles were added to human WB culture system in serial concentrations to stimulate the cells. PHA was used as positive control. After 24 h, all the cultural supernatants were harvested and the concentrations of Th1 (IL-2, IFN-γ and TNF-α) and Th2 (IL-4, IL-6 and IL-10) cytokines released to the supernatants were detected by cytometric bead array (CBA). The percentage of CD69 expression on CD4+T cells from WB was detected by immuno-fluorescence staining plus flow cytometry. RESULTS: The concentration of p24 antigen in the AT-2-inactivated specimen was 85.5 μg/L. 24 h later, the percentage of CD69 expression on CD4+T cells from control group was (1.62±0.63) %, whereas it was (38.82±6.00)%, (3.83±1.07)%, (5.94±0.85)% and (9.30±1.22)% in PHA group, HIV-1 (1/500) group, HIV-1 (1/50) group and HIV-1 (1/5) group, respectively. Cytokines secreted by WB in control group were mainly TNF-α and IL-6. However, all the six cytokines tested were strikingly increased in PHA group, as well as in HIV-1ⅢB groups. CONCLUSION: AT-2-inactivated HIV-1ⅢB particles activate CD4+T cells from WB, and up-regulate both Th1 and Th2 cytokine secretion in WB. Besides the effects of viral proteins, other mechanisms may be proposed that HIV-1 particles act as antigen presenting cell (APC) because many host-derived immune molecules are incorporated into HIV-1 envelop when it is released from infected cells by budding, and exert immune modulation.  相似文献   

18.
AIM: This study was designed to use RNA interference technique to down-regulate the expression of survivin gene in human Burkitts lymphoma cell line Daudi and to explore the effect on sensitivity of Daudi cells to adriamycin. METHODS: The survivin-shRNA expression vector was constructed and transfected into Daudi cells. Expression of survivin mRNA and protein were assessed by RT-PCR and Western blotting analysis, respectively. Apoptosis index of transfected Daudi cells was quantified by flow cytometry. The sensitivity of Daudi cells to adriamycin (ADR) before and after transfection was detected by MTT test. RESULTS: The mRNA and protein levels of survivin were down-regulated by 62.32% and 61.88%, respectively, compared to those in control-shRNA treated group and PBS treated group (P<0.05). Meanwhile, the apoptosis index was significantly increased (19.10%±2.15%), compared to that in control group (4.48%±1.54%) and PBS group (4.35%±1.37%, P<0.05). The 50% inhibition concentration (IC50) of ADM to Daudi cells was significantly decreased (0.25±0.43) μmol/L, compared to that in control group (0.87±0.21) μmol/L and PBS group (0.91±0.36) μmol/L, P<0.05. CONCLUSION: Down-regulation of survivin expression in Daudi cells by shRNA effectively induces apoptosis and increases the sensitivity of Daudi cells to ADR.  相似文献   

19.
AIM: To investigate the effect of bone marrow mesenchymal stem cell (BMSC) transplantation on learning and memory abilities and pathological changes of Alzheimer disease (AD) mice and the molecular mechanisms. METHODS: C57/BL6 wild-type (WT) and transgenic (Tg) mice were randomly divided into 4 groups:WT/PBS group, WT/BMSCs group, Tg/PBS group and Tg/BMSCs group. The mice were administered with PBS or BMSCs via intracerebroventricular injection. Spatial learning and memory abilities of the mice were evaluated by Morris water maze test on the 3rd day after surgery. Real-time PCR was applied to detect the mRNA expression of CX3C chemokine ligand 1 (CX3CL1), CX3C chemokine receptor 1 (CX3CR1), IL-1β, TNF-α, Nurr1, YM1, insulin-degrading enzyme (IDE) and matrix metalloproteinase 9 (MMP9). The protein levels of CX3CL1 and Aβ42 were measured by ELISA. Western blot was used to detect the protein expression of postsynaptic density protein 95 (PSD95) and synaptophysin (SYP). RESULTS: The transplanted BMSCs were observed near the hippocampus of APP/PS1 mice on the 10th postoperative day. The escape latency of the mice in Tg/PBS group was significantly longer than that in the WT/PBS mice (P<0.05). Compared with Tg/PBS group, the escape latency of Tg/BMSCs group was significantly shorter (P<0.05), and the mRNA and protein levels of CX3CL1 in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.01). The results of immunohistofluorescence staining showed that BMSC transplantation promoted the activation of microglia in the brain of WT and Tg mice. The mRNA expression of YM1 was up-regulated in WT/BMSCs group and Tg/BMSCs group (P<0.05). Compared with WT/PBS mice, the mRNA expression of TNF-α in the cortex and hippocampus of Tg/PBS group was significantly increased (P<0.05), and the mRNA expression of Nurr1 in the cortex was significantly decreased (P<0.01). Meanwhile, the mRNA expression of TNF-α in the cortex of Tg/BMSCs mice was decreased (P<0.01) and the mRNA expression of CX3CR1 and Nurr1 was up-regulated compared with Tg/PBS group (P<0.05). The results of Western blot showed that the protein levels of PSD95, p85, p110 and p-Akt in Tg/BMSCs group were significantly higher than those in Tg/PBS group (P<0.05). Finally, BMSC transplantation reduced the protein level of Aβ42 in APP/PS1 mice (P<0.05), and increased the mRNA expression of IDE and MMP9 in the hippocampus (P<0.05). CONCLUSION: BMSC transplantation modulates neuroinflammatory responses and promotes neuroprotective factor and synaptic protein expression, thus improving the learning and memory abilities in the APP/PS1 mice, which may be achieved by up-regulating the expression of CX3CL1.  相似文献   

20.
AIM：To explore the effect of mesenteric lymph duct ligation against actue lung injury (ALI) in rats.METHODS：45 Wistar rats were divided into three groups：the ligation group,the non-ligation group and sham operated group,and the two-hit model was established by hemorrhage and LPS injection.Mesenteric lymph was diverted by ligating mesenteric lymph duct in ligation group.All rats facilitated blood withdrawal for blood sample to arterial gas analysis after 24 hours.Then the WBC,NO,NOS,MDA,SOD and lung permeability index (LPI) were determined in bronchoalveolar lavage fluid (BALF),the MPO and ATPase activity were determined in lung homogenate.The ultrastructure was also observed.RESULTS：After two-hit,the PaCO2,the total cells and PMN,the NO2-/NO3-,NOS and MDA content in BALF and MPO activity in lung homogenate and LPI in non-ligation group were significantly increased than those in sham operated group.PaO2 and pH in arterial blood,SOD in BALF and the ATPase in lung homogenate were significantly lower (P<0.01 or P<0.05).The total cells and PMN,MDA,NO2-/NO3- in BALF,LPI in ligation group were significantly increased than those in sham operated group,and SOD in BALF was significantly lower (P<0.01 or P<0.05).The pH and PaO2 in arterial blood,the ATPase in lung homogenate in ligation group were significantly increased than those in non-ligation group,and the PaCO2,the total cells,PMN,NO2-/NO3-,NOS,MDA in BALF,LPI,and MPO in lung homogenate in ligation group were significantly lower than those in non-ligation group (P<0.01 or P<0.05).The injury of pulmonary vascular endothelium in ligation group was lighter than that in non-ligation group.CONCLUSION：The ligation of mesenteric lymph duct attenuates the ALI of rats.Mesenteric lymph might play an important role in the pathogenesis of ALI.  相似文献   

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