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The effects of combinations of salinity (no salt, 2000 p.p.m. or 4000 p.p.m. of CaCl2 and NaCl, 1:1) and water table (30 cm, 60 cm or 90 cm from the soil surface), on the vegetative growth and tolerance of ‘Golden Japanese’ plum and ‘Mit Ghamre’ and ‘Balady’ peaches were studied. The plants were grown in lysimeters. The growth of the trunk, total shoot length, the increase in shoot length per cm and the fresh weight of top, root and total plant were reduced with increasing salinity of the irrigation water. The effect was accentuated when the plants were maintained at high water table level. The salinity treatments resulted in the death of 43%, 73% and 76% of the plants in the plum, and the ‘Mit Ghamre’ and ‘Balady’ peaches, respectively, indicating that the plum is more tolerant to salinity than the peach. The plants of the salinity treatments showed various symptoms of salt injury, such as leaf burn, defoliation, shoot die-back and finally death. In the peaches, salt injury started to occur in the first growing-season, whereas the salt injury appeared in the plum in the second growing-season. The symptoms were more pronounced in the 4000 p.p.m. treatment than in the 2000 p.p.m. treatment and were more pronounced at the high water table level. The salinity level was the predominant factor and the effect of the water table on the vegetative growth diminished with increase in the salinity level of the irrigation water. 相似文献
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AIM:To establish a fast, accurate and economical technique for culturing mouse pulmonary arteriolar smooth muscle cells (PASMCs), and to explore the effects of hypoxia on the proliferation and apoptosis of the PASMCs. METHODS:In sterile condition, the pulmonary artery was isolated from the male BALB/c mice by digesting with collagenase I, and the cells were cultured in fetal bovine serum-coated flask. Centrifugal procedure was not used during the cell passage. The cell morphology was observed under an inverted phase-contrast microscope. α-Smooth muscle actin was identified by immunocytochemistry and immunofluorescence. The effects of hypoxia on the proliferation and apoptosis of the PASMCs were detected by CCK-8 assay and TUNEL assay. RESULTS:PASMCs were identified by the methods of immunocytochemistry, immunofluorescence staining and observation of morphology. Unlike the rat PASMCs with typical subcultured peak-vally pattern, the mouse PASMCs showed a lot different without a peak-vally pattern. The cells could be subcultured after 5 d to 7 d and there was 3 to 5 generations depending on the activity of the cells. CCK-8 assay demonstrated that the A values of PASMCs exposed to hypoxia increased after 24 h (P<0.05) as compared with normoxia. TUNEL result showed that the apoptotic index of the PASMCs in hypoxia decreased after 24 h (P<0.05). CONCLUSION:This technique for obtaining cultured mouse PASMCs is simple, fast, accurate and economical. The digestion time is easy to control. Hypoxia promotes the proliferation and inhibits the apoptosis of PASMCs. 相似文献
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小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备 总被引:2,自引:0,他引:2
研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。 相似文献
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钾肥在小白菜和萝卜上的施用效果 总被引:6,自引:0,他引:6
采用盆栽试验方法研究了施用钾肥对小白菜、萝卜生长发育、产量、品质及钾素含量的影响。结果表明 ,施用钾肥对小白菜和水萝卜的株高、叶片数无显著的影响 ,但可提高水萝卜块根的产量 ,提高幅度为 31.0 4 %~ 97.93%。施用钾肥可提高小白菜和萝卜的蛋白质和VC含量 ,降低硝酸盐含量 ,增加植株的钾素含量 相似文献
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Fresh onion seeds desiccated to 6.0% seed moisture content (SMC) were stored in various packaging materials under different storage conditions. Seeds packed in aluminum-laminated pouches beside those stored with silica gel at 25 °C maintained satisfactory germinability and vigour after 12 months. Desiccated seeds stored in moisture impervious containers produced more vigourous seedlings. Germination potential of onion seeds increased with reduced SMC besides storage in moisture impervious packets along with desiccants as physiological and biochemical attributes are regulated. Seed viability and vigour decreased with accelerated ageing due to increased lipid peroxidation, decreased activities of several free radical and peroxide scavenging enzymes. Electrical conductance of seed leachates also increased with ageing. Thus, adoption of appropriate storage temperature and moisture control techniques would significantly affect onion seed quality, which was due to minimum accumulation of free peroxide radicals and enhanced activity of free radical scavenging enzymes. 相似文献
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以大荔县冬枣种植区采集的发病枣果为试材,采用常规组织分离法进行病原菌的分离纯化,通过柯赫氏证病法则、形态学观察及分子生物学分析,研究鉴定了冬枣黑斑病和炭疽病的病原菌种类,并采用田间调查法对2种病害为害特征进行比较分析,以期为田间有效区分冬枣黑斑病和炭疽病为害症状,并快速判断其病原类型,及进一步深入开展精准防控提供参考和指导.结果表明:陕西大荔冬枣黑斑病的病原菌为细极链格孢(Alternaria tenuissima),炭疽病的病原菌为胶孢炭疽菌(Colletotrichum gloeosporioides).2种病害均主要为害枣果实,在枣果果腰或果肩上形成黑褐色病斑,可能同时存在且症状相近极易混淆,但在病斑表面、皮下及久置表型3个方面存在明显区别. 相似文献
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大棚葡萄种植过程中,容易产生多种病虫害,如黑痘病、炭疽病等。针对当前山西省大棚葡萄的种植情况,本文分析了大棚葡萄常见的病虫害,重点阐述了病虫害防治技术,如物理防治、化学防治、农业防治、生物防治等,为大棚葡萄病虫害的科学防治提供参考。 相似文献
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The early-1990s’ war in former Yugoslavia led to severe damage to the urban and peri-urban forests of Sarajevo, capital of Bosnia and Herzegovina. During the Siege of Sarajevo (April 1992–March 1996), after the energy supplies to the city had been cut off, over three-quarters of all urban trees and nearly all peri-urban trees within the siege line were cut down for firewood by the desperate residents. After the war, the city trees were quickly and effectively replanted, and Sarajevo provides a useful case study of urban forest recovery. This study presents the observations and measurements, of trees that survived the war and the trees planted after the war, made in Sarajevo in May 2008. We summarize the lessons learned from the Sarajevo experience, regarding both damage and replanting, which include the close relationship between the urban tree damage and the specifics of military operations, the importance of the initial planting stock, and the advantages of collaboration between academic researchers and urban forest managers. 相似文献
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WANG Kai-fu LU Fu-er XU Li-jun YANG Ming-wei ZOU Xin LI Ming-zhen YE Wang-yun 《园艺学报》2006,22(1):135-138
AIM: To explore the therapeutic strategies of heat-clearing and detoxifying (HCDT) and Yin-invigorating and fluid-supplementing (YIFS), the method of Chinese medicine, on the high density lipoprotein (HDL) and alkaline phosphatase (ALP) molecules, which are associated with endotoxin-degradation. METHODS: The animal model of endotoxemia was established by the injection of E. Coli lipopolysaccharides through rabbits ear vein. The endotoxemic rabbits were treated respectively with two representative herbal preparations of therapeutic principles against febrile diseases: HCDT or YIFS preparations. The serum levels of interleukin-1 (IL-1), tumor necrosis factor α (TNF-α) and HDL, the ALP activity, the expression of ALP mRNA in liver and kidney tissues were observed. RESULTS: The serum levels of IL-1 and TNF-α in HCDT group were significantly decreased, while the serum ALP activity and the expression of ALP mRNA in liver and kidney tissues were obviously increased, as compared with those in model group. Meanwhile, the serum levels of IL-1 and TNF-α in YIFS group were significantly reduced, and its plasma HDL level was elevated. CONCLUSIONS: Both the herbal preparations, HCDT and YIFS, have the scavenging effects on the overproduction of inflammatory cytokines such as IL-1 and TNF-α induced by endotoxin, but their effects on the endotoxin-degrading molecules might be different. HCDT principally increases ALP activity and enhances ALP expression in liver and kidney tissues, while YIFS might preferably facilitate the elevation of plasma HDL level. 相似文献
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应乌克兰农业科学院哈尔科夫瓜类蔬菜研究所的邀请,由新疆哈密瓜研究中心组成赴乌克兰科技考察团,以吴明珠院士为团长,考察团成员还有新疆哈密瓜研究中心伊鸿平研究员、合肥华夏西瓜甜瓜育种家联谊会副会长杨鼎新副研究员与秘书长陈会中高级农艺师、中国农业科学院郑州果树研究所徐志红副研究员。考察团于2004年9月10-25日对乌克兰农业科学院所属的北南2个瓜类蔬菜研究所进行了访问,对该国瓜类(蔬菜)科研生产进行了初步考察。此次考察的目的是了解乌克兰瓜类(蔬菜)科研生产状况,加强联系,建立瓜类(蔬菜)科技合作与交流关系,取长补短,以促进… 相似文献
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1、核桃溃疡病 1.1危害症状溃疡病真菌病害,主要危害核桃树幼树主干、嫩枝和果实,初为褐、黑色近圆形病斑,直径0.1-2cm,多发生在树干及主侧枝基部.有的扩展成菱形或长条形病斑.在幼嫩及光滑的树皮上,病斑呈水渍状或为明显的水泡,破裂后流出褐色粘液,遇空气变黑褐色,随后病部散生许多小黑点,严重时病斑相连呈菱形或长条形.当病部扩大到环绕枝、干一周时,出现枯梢、枯枝或整株死亡.秋季病部表皮破裂.在较老的树皮上,病斑多呈水渍状,中心黑褐色,病部腐烂深达木质部.果实受害后呈大小不等的褐色圆斑,早落、干缩或变黑腐烂. 相似文献
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