共查询到19条相似文献，搜索用时 873 毫秒
1.
乐都山羊红细胞钾型的研究 总被引：7，自引：3，他引：4
采用火焰光度法对７５只乐都山羊的红细胞钾型进行了调查研究。结果发现：①按红细胞钾浓度，乐都山羊有高钾和低钾两种表型，以高钾为优势表型（８８％）；②高钾型山羊的红细胞钾浓度在４３．５９￣８１．３０ｍｍｏｌ／Ｌ之间，低钾型的在９．３７￣２１．２１ｍｍｏｌ／Ｌ之间；③Ｋ＾ｈ和Ｋ＾Ｌ等位基因频率分别为０．９３８７和０．０６１９，基因杂合度为０．１１６１。 相似文献
2.
采用火焰光度计法测定了湟源县日月乡黄牛４０份血样的血钾浓度。测定结果为：全血钾浓度９．１６ｍｍｏｌ／Ｌ；红细胞钾浓度１９．７９ｍｍｏｌ／Ｌ；血清钾浓度４．３５ｍｍｏｌ／Ｌ。全血钾浓度和红细胞钾浓度呈显著的正相关关系（ｒ＝０．９４４１Ｐ＜０．００１） 相似文献
3.
柴达木黄牛和青海东部黄牛红细胞钾型的研究 总被引：3，自引：0，他引：3
采用火焰光度法对２３８头柴达木黄牛和２６３头于海东部黄牛的红细胞钾型进行了研究。结果发现：①柴达木黄牛全部为低血钾型，而青海东部黄牛有高血钾和低血钾两种表型；②青海东部黄牛的Ｋ＾Ｌ和Ｋ＾ｈ基因频率分别为０．８２６和０．１７４。 相似文献
4.
互助白牦牛红细胞钾浓度多态性的研究 总被引：3，自引：0，他引：3
采用火焰光度计法对７０头互助白牦牛的红细胞钾浓度多态性进行研究。结果表明：①被检白牦牛有高血钾和低血钾两种表型，以高血钾型为优势表型（８５．７１％）；②Ｋ＾ｈ和Ｋ＾Ｌ等位基因频率分别为０．９２５８和０．０７４２；③白牦牛红细胞钾浓度基因座的有效等位基因数、基因杂合度和基因均质度指数分别为１．１５９３，０．１３７４和０．７５２。 相似文献
5.
采用火焰光度法测定了德令哈地区柴达木黄牛９５份血样的血钾浓度常值，它们的平均全血钾浓度为８．４３±１．５１ｍｍｏｌ／Ｌ；红细胞钾浓度为１３．１±３．１３ｍｍｏｌ／Ｌ；血清钾浓度为５．１９±０．６７ｍｍｏｌ／Ｌ。 相似文献
6.
红细胞钾含量对奶牛耐热性的影响 总被引：14，自引：2，他引：12
１９９５年１～８月，我们测定了江西省畜牧良种场５４４头泌乳牛的红细胞钾含量，并根据其出生年月、胎次、产犊日期、产奶量和红细胞钾含量选出２２头，分成高钾（ＨＫ）和低钾（ＬＫ）两组进行耐热性观察。结果表明ＬＫ组产奶量下降率极显著低于ＨＫ组（Ｐ＜０．０１），ＬＫ组较ＨＫ组耐热。ＬＫ组直肠温度（ＲＴ）低于ＨＫ组，但差异不显著（Ｐ＞０．０５）。 相似文献
7.
采用火焰光度法对海北州牧科所优良种畜繁殖场，９０只夏洛来杂种羊的红细胞钾型进行了研究，结果发现夏洛来杂种羊红细胞钾浓度存在多态性，存在ＨＫ和ＬＫ两种类型，高钾型羊３５只（３８．８９％），低钾型羊５５只（６１．１１％），Ｋｈ和ＫＬ等位基因频率分别为０．６２３６和０．３７６４。 相似文献
8.
青海半细毛羊红细胞钾型的研究 总被引：3，自引：1，他引：2
采用火焰光度法对青海半细毛羊的红细胞钾型进行了研究。结果发现：青海半细毛羊的红细胞浓度存在ＨＫ和ＬＫ两种表型，其频率分别为２２．２２％和７２．７８％，Ｋ＾ｈ和Ｋ＾Ｌ等位基因频率分别为０．４７１４和０．５２８６。 相似文献
9.
大通家牦牛和含野血牦牛红细胞钾型的研究 总被引：3，自引：0，他引：3
采用火焰光度法对１０９头大通家牦牛，１１０头１／４野血牦牛和９３头１／２野血牦牛的红细胞四型进行了研究，结果表明：（１）大通家牦牛，１／４和１／２野血牺牦牛都有ＨＫ和ＬＫ两种表型，以ＨＫ型为优势表型；（２）３个牦牛群体Ｋ和Ｋ基因频率分别为０．０５１８，０．０５１３，０．０５５３和０．９４８２，０．９４８７，０．９４４７；（３）３个群体的基因分化系数为０．０００５０，表明导入野牦牛血对大通牦牛红细胞 相似文献
10.
青海细毛羊红细胞钾型的初步研究 总被引：10，自引：0，他引：10
采用火焰光度测定法对青海细毛羊红细胞钾型进行了研究。结果发现：青海细毛羊红细胞钾浓度存在多态性，ＨＫ和ＬＫ型羊分别占８．２７％和９１．７８％，Ｋ＾ｈ和Ｋ＾ｌ等位基因频率分别为０．２８６７和０．７１３３，同时还讨论了绵羊钾型与血红蛋白型、血清钾和钠浓度的关系。 相似文献
11.
柴达木改良绒山羊红细胞钾型的研究 总被引：1，自引：0，他引：1
采用火焰光度法对94只柴达木改良绒山羊的红细胞钾型进行了调查研究。结果发现：（1）柴达木改良绒山羊红细胞钾浓度存在多态性，有高钾和低钾两种表型，以高钾型为优势表型（79．79％）；（2）高钾型山羊的红细胞钾浓度在37－83.2mmol/L之间，低钾型的在16－28．8mmol/L之间；（3）K^L和K^h等位基因频率分别为0．1068和0．8932，基因杂合度为0．1782。 相似文献
12.
Six HK (high erythrocyte potassium) and 7 LK (low erythrocyte potassium) dairy cows were subjected to a 4-h intravenous infusion of 4.7% Na2EDTA solution to induce and maintain hypocalcaemia. Blood samples taken immediately before infusion, hourly for 7 h, and at 24 h after commencement of infusion were subjected to determination of concentration (or count) of 16 analytes. The mean changes in concentrations (or counts) of the various blood analytes were calculated for the periods 0-4, 4-7, 7-24, and 0-24 h after commencement of the infusion for all cows combined, and then separately for the HK and LK groups of cows. Plasma Ca(PCa), plasma inorganic phosphorus (PiP) and plasma potassium (PK) showed significant decreases during the 4-h infusion period and were still below pretreatment levels 24 h later. AST, CPK, PCVs and white cell-counts (WCCs) showed significant early increases which were still significantly elevated 24 h later. Plasma magnesium (PMg) and erythrocyte Na(ENa) and K(EK) all showed delayed changes which still persisted 24 h later. Significant between-group differences were present for PCVs which increased significantly more in the LK than the HK group during the infusion period, for PCa which showed a greater increase in the HK cows than the LK cows during the 4-7 h early clinical recovery period, and for plasma bilirubin (PBil) which showed a greater increase from 0 to 24 h in the HK group than in the LK group. Urine samples, collected before infusion, 4-7 h and 24 h after commencement of the infusion, were subjected to analysis for glucose, protein, pH, 'blood' and ketones. Most cows showed increases in urinary glucose, protein and 'blood'. 相似文献
13.
Yamato O Lee KW Chang HS Tajima M Maede Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(10):1179-1182
The concentrations of sodium, potassium, reduced glutathione (GSH) and free amino acids and Na-K-ATPase activity in erythrocytes were examined in 35 purebred Jindo dogs in Korea. The incidence of Jindo dogs with a high potassium concentration and high activity of Na-K-ATPase in erythrocytes (HK phenotype) was 25.7%. The erythrocyte GSH concentration in HK Jindo dogs varied widely, from 2.45 to 12.38 mmol/l of RBCs, and was positively correlated with the erythrocyte glutamate concentration. These results indicate that HK Jindo dogs have normal to very high levels of erythrocyte GSH, which might result from the varying quantity of Na-dependent glutamate influx in the erythrocytes. 相似文献
14.
采用火焰光度法对海南州９０只藏羊的红细胞钾型进行了研究。结果：高钾型羊６３只（７０％），低钾型羊２７只（３０％），Ｋｈ和ＫＬ等位基因频率分别为０．８３６７和０．１６３３ 相似文献
15.
青海藏獒血液蛋白质和红细胞钾浓度多态性的研究 总被引：2，自引：0，他引：2
采用聚丙烯酰胺凝胶电泳法和火焰光度计法对２１条青海藏獒的血红蛋白，亲血蛋白和红细胞钾浓度的多态性进行了研究。结果表明：１．藏獒的血红蛋白呈单态，现显单一的ＨＢＢＢ型，基相对迁移率为３９．２９％；２．亲血色蛋白有ＨＰ＾＋和ＨＰＯ两种表型；其表型频率分别为０．９０４８和０．０９５２；（３）红细胞钾浓度呈现单一的低血钾型。 相似文献
16.
青海绵羊红细胞钾型的研究 总被引：3，自引：0，他引：3
采用火焰光度法对青海省8个品种绵羊的红细胞钾型多态性特征进行了调查研究。结果发现,被检的8个品种绵羊均有高钾(HK)和低钾(LK)两种表型而呈现多态性,其中欧拉羊、藏羊、小尾寒羊以HK型为优势表型,新疆细毛羊、青海细毛羊、青海半细毛羊以LK型为优势表型;绵羊的红细胞钾型没有性别差异;红细胞钾型与生产性状指标间没有显著性关系;在被检的15项血液指标中,除WBC,BK值和EK值差异显著(P<0.01)以外,其他血液指标没有型间差异(P>0.05);不同红细胞钾型绵羊的HB型分布非常相似(P>0.05)。 相似文献
17.
Energy metabolism in canine erythrocytes associated with inherited high Na+- and K+-stimulated adenosine triphosphatase [(Na,K)-ATPase] activity (HK cells) was compared with that in normal canine erythrocytes (LK cells). Activities of some of the glycolytic enzymes in the HK cells were significantly higher than those in LK cells. The concentrations of adenosine triphosphate (ATP) and glycolytic intermediates in HK cells were almost equal to those in LK cells. Glucose utilization and lactate production by HK cells in vitro and incorporation of [32P]orthophosphate or [14C]glucose into 2,3-diphosphoglycerate in HK cells were higher than in LK cells. Radioactivity of [32P]ATP in HK cells was lower than in LK cells, but increased to approximately that of LK cells when (Na,K)-ATPase of HK cells was completely blocked by ouabain. When HK cells and LK cells were incubated in the absence of glucose, the concentration of ATP in HK cells was decreased more than that of LK cells. Although ouabain reduced the rate of decrease in ATP in HK cells, the decrease in ATP in HK cells was still 2-fold that in LK cells. The half-life of HK cells was about one-half that of LK cells. The results indicated that glycolysis is greater in HK cells than in LK cells, and that the increased glycolysis in HK cells was stimulated by an increased rate of ATP breakdown for active cation transport by the (Na,K)-ATPase and by increased degradation of ATP for some other pathway, eg, glutathione synthesis. Thus, the increased demand for ATP in HK cells might result in shortening the lifespan of HK erythrocytes. 相似文献
18.
Y Maede Y Amano A Nishida T Murase A Sasaki M Inaba 《Research in veterinary science》1991,50(1):123-125
The sodium (Na) and potassium (K) concentrations and Na, K-ATPase activity were examined in erythrocytes from 24 Japanese shiba dogs and 79 dogs of 24 other breeds. Eleven of the shibas had erythrocytes with high K and low Na concentrations, together with high Na, K-ATPase activity (HK RBCs), while red cells from the remaining shibas and all of the other breeds examined showed low K and high Na concentrations, with no enzyme activity (LK RBCs). The concentration of reduced glutathione in HK RBCs was about five times that in LK RBCs. All the findings from HK shibas were in good agreement with those from HK mongrel dogs found in Japan previously. Since the shiba is a Japanese breed of dog, the results of the present study strongly suggest that the gene for HK RBCs may be inherent in dogs indigenous to Japan, particularly in shiba dogs. 相似文献
19.
E Ogawa A Kawakami T Yagi T Amaya H Fujise R Takahashi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(1):57-62
Oxidative damage to the membrane in canine erythrocytes with inherited high Na, K-ATPase activity (HK cells) was compared with that in normal canine cells (LK cells). When 30 mM beta-acetylphenylhydrazine (APH) was applied to HK and LK cells, lipid peroxidation and hemoglobin denaturation occurred. Lipid peroxidation determined from malondialdehyde (MDA) formation was significantly lower in HK than in LK cells so far as endogenous glutathione (GSH) concentration was maintained at appropriate levels. With the depletion of GSH, MDA formation was accelerated and difference between HK and LK cells was not significant. Denatured hemoglobin bound to the membrane protein was less in HK than in LK cells. During incubation with APH, osmotic fragility increased markedly in LK cells, while HK cells showed very little change. The amounts of total lipid, total and free cholesterol, glycolipid, phospholipid and fatty acids were essentially the same in both cell types. Fatty acid compositions showed very small differences. The membrane of HK cells thus appear to have greater protection against oxidative damage induced by APH, owing to the presence of excess GSH in HK cells. The capability of HK cells to withstand oxidative damage would not be due to differences in membrane lipid compositions. 相似文献