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1.
Segregation of a wide range of body colour in the progenies was observed in breeding experiments with the Taiwanese red tilapias. Homozygous stocks of pink, red and golden colour morph were selected from such a stock by brother-sister crossing for six generations. DNA fingerprinting analysis using multilocus M13 and Bkm 2(8) probe and HinfIenzyme combinations of pink individuals, showed homozygosity in their genome. Pink individuals when crossed to black Oreochromis mossambicus (Peters) resulted in all pink F1 progenies. F1 X F1 crosses resulted in segregation of pink and black in the F2 progenies in a 3 : 1 ratio. F1 pink individuals when backcrossed to O. mossambicus gave progeny in the ratio of 1: 1 of pink and black. Black individuals from the F2 generation, when crossed to O. mossambicus and pink separately, resulted in all black and all pink progenies, respectively. These results show that the pink colour is dominant over the black phenotype and is inherited as an autosomal locus with complete penetrance.  相似文献   

2.
The present study was undertaken with a view to compare the precision and reliability of the age readings obtained from different bony structures of some important freshwater teleosts viz., Labeo rohita (Hamilton), Catla catla (Hamilton) and Channa marulius (Hamilton). Standard procedures were followed to prepare and study the age structures. In L. rohita and C. marulius percent agreement between reader's age estimates was highest for scales, i.e. 96.3% and 90.5%, respectively and in C. catla percent agreement was highest (93.3%) for opercular bone. When scale ages were compared with other alternative structures viz., otoliths, opercular bone, vertebral centra and dorsal fin rays, percent agreement was found highest between scale and opercular bone age estimates (77.8%) in L. rohita and between scale and otoliths (94.8%) in C. marulius. In case of C. catla highest percent agreement was found between opercular bone and scale age estimates. In L. rohita each of the ageing structure showed significant (P < 0.05) underestimation of age in comparison to scales. In C. catla mean age estimates from opercular bone were comparable (P > 0.05) to the values obtained from all other structures except dorsal fin rays. In C. marulius mean age estimates from scales were comparable (P > 0.05) to those from all other structures except from dorsal fin rays. Results indicated scales to be the most suitable structure for ageing L. rohita and C. marulius and opercular bone for C. catla. However, in C. catla also scales may be used as a non-destructive method of age estimation with satisfactory results.  相似文献   

3.
Culture water and various tissues from carps (Catla catla (Hamilton), Labeo rohita (Hamilton), Cirrhinus mrigala (Hamilton) and Cyprinus carpio L., respectively) grown in an aquaculture pond in Tiruchirappalli, India, were examined for the presence of Enterobacteriaceae. Bacterial species isolated from the intestinal tract of the four fish species were similar to those isolated from the pond water. More than 50% of the total bacterial population identified in both the pond water and fishes intestinal tract were Escherichia coli and Pseudomonas aeruginosa. The presence of these bacteria suggests the need for following strict hygiene during handling and processing fish to prevent the transfer of potentially pathogenic bacteria to humans. Additionally, it stresses the need for a code of practice for fish growers in aquaculture systems for safety reasons.  相似文献   

4.
The spatial and seasonal variability of the respiratory enzyme succinate dehydrogenase and the protein content were examined in different tissues of fish cultured in three ponds along the effluent gradient of a sewage-fed fish farm. Indian major carp Catla catla (150-230 g) and Labeo rohita (60-190 g) cultured in both the middle and last points of the sewage effluent (stocking pond 1) and (stocking pond 4) and Oreochromis mossambicus (50-160 g), a naturally growing fish of the inlet (facultative pond) and the out let of the sewage effluent (stocking pond 4) were procured every month during the period of January-December, 2005 and were subjected to determination of succinate dehydrogenase activity, total protein, DNA and RNA contents from gill, liver and muscle tissue respectively. The SDH activity of all three test fishes (Catla catla, Labeo rohita and Oreochromis mossambicus) was reduced significantly (ANOVA; P < 0.05) when cultured in SP-4 compared to SP-1 in the case of Catla catla and Labeo rohita and in facultative pond in the case of Oreochromis mossambicus.Conspicuous differences in the SDH activity of fish between the last and first stocking pond or the facultative pond were clearly due to the result of the differences in water quality. There was a direct relationship between SDH activity in gill tissue of any of the fish investigated and ammonia-N concentration of water or water pH. This shows that the respiratory activity of these fishes was strongly affected by the ammonia and pH of water. In other words, this suggests that as the distance from the point source increases, there was a substantial improvement of water quality in the ponds located along the sewage effluent gradient. Evidently, there is a progressive pattern of growth, survival and physiological health of fish and abundance of favorable diversity of food organisms with rich biodiversity.  相似文献   

5.
An autoregressive analysis was performed on data from an investigation of the velocity and acceleration of the day-to-day length increments of two Indian major carp, Catla catla (Hamilton) and Labeo rohita (Hamilton), hatched in artificial (Chinese-style hatchery) and semi-natural (improvised pits) holding conditions. The autoregressive model, which was capable of providing short- and long-term predictions, was tested in parallel with a time regressive model and an instantaneous growth rate model. The estimate simulating short-term predictions was found to describe the data more efficiently than the other estimates of the models. Product-moment correlations comparing the two holding systems revealed no source-specific variation of growth between the species. However, the significant length velocity correlation suggests that the artificial system has greater efficacy.  相似文献   

6.
This paper describes an experiment using a recirculatory-filtering system operating in two rectangular cemented ponds (160 and 190 m2, 1.5 m deep) to rear fry (25 mm–50 mm) of the major Indian carp species Labeo rohita (Hamilton) and Catla catla (Hamilton) to fingerlings (100 mm–125 mm). These operations were concluded in both the species in 32 days at stocking densities of 0.46 million/ha and 0.55 million/ha, and showed survival rates of 96.6% and 84.4% respectively. In the presently followed practices of modern fish culturists, corresponding operations in earth ponds usually take 90 days and have a stocking density of 0.2 million/ha with survival rates more or less similar to those obtained in the recirculatory-filtering system. If followed by industry, the technique described will enable production of major Indian carp fingerlings with great saving in time, the use of water and the space occupied by the production system. These advantages greatly enhance the economic viability of rearing carp fingerling in India.  相似文献   

7.
Among three tilapiine species, Oreochromis aureus (Steindachner), O mossambicus (Peters) and O. niloticus (L.), fifteen banding positions of haemoglobin were resolved using citric acid-aminopropylmorpholine pH 7.0 buffer. O. mossambicus and O. aureus displayed 7 and 8 fixed band locations, respectively, whereas eight strains of O. niloticus from Asian cultured and African wild populations showed a total of 14. Variability in the O. niloticus strains, manifested as presence or absence of haemoglobin bands, was observed in banding positions 3, 4, 6, 7, 8, 9, 10, 13 and 14. Six banding positions in O. niloticus were shared with O. mossambicus and 8 with O. aureus. A dendrogram constructed from distance coefficients between popu-lation pairs using the presence or absence data showed the close affinity among O. niloticus strains belonging to the O. niloticus niloticus subspecies. A slight separation of Kenya O. niloticus from the O. niloticus niloticus strains is consistent with its subspecific distinction as O. niloticus vulcani. Among three species, O. niloticus had a closer affinity to O. aureus than to O. mossambicus. The phylogenetic relation-ships using differences in haemoglobin patterns and the higher genetic variability of O. niloticus reflect similar results obtained from existing studies using allozymes. In general, the presence of multiple fish haemoglobins is attributed to their adaptability to haeterogeneous environments. This attribute is typical of tilapias.  相似文献   

8.
The present study investigates the success of stocking fingerlings of Indian major carps ‐Gibelion catla (Hamilton), Labeo rohita Hamilton and Cirrhinus mrigala Hamilton – to enhance fish yield in 58 small reservoirs in Odisha State, India. Fish stocking and yield data were collected from State Fisheries Department, Odisha. The relationships between area, stocking density and fish yield of different size groups of reservoirs were assessed using the Pearson correlation coefficient (r). Fish yield increased significantly (p < 0.05, t‐test) from 204 kg/ha/year in 2012–13 to 323 kg/ha/year in 2013–14 due to adoption of CBF. The corresponding per capita fish production increased from 398 kg fisher?1 year?1 to 702 kg fisher?1 year?1 benefitting more than 8,000 fisher households. Reservoirs in the size group 100–500 ha showed the highest response of fish yield with respect to stocking of fingerlings. The size of fingerlings at stocking and area of the reservoir had greater impact on enhancing fish yield. Stocking had a profound positive impact on fish yield. This study forms a baseline evaluation of the impact of stocking on small tropical reservoirs in India. It also recommends management measures and discusses issues, and the way forward for sustainable fisheries enhancement in small tropical reservoirs in eastern India.  相似文献   

9.
Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot‐1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot‐1 DNA – which contains highly and moderately repetitive DNA – was extracted from genomic DNA, labelled as a probe with Dig‐11‐dUTP, and in situ hybridized to spreads of mitotic chromosomes of the three samples. The hybridized signals were detected by means of Cy3‐conjugated antidigoxigenin. The FISH results indicated that the three samples had the same diploid number (2n=44) of chromosomes. Specific fluorescence signal bands were detected on all individual chromosome pairs. On the basis of Cot‐1 DNA FISH banding patterns and chromosome morphology, the karyotypes of the three samples have been constructed; no remarkable differences were detected between the karyotypes of these species using this method. These results – which are similar to those reported previously, with respect to chromosome number, morphology and Cot‐1 DNA FISH patterns – suggest chromosomal stasis during speciation and hybridization of tilapia (Oreochromis, Cichlidae). Such a molecular cytogenetic procedure, if used in conjunction with other genomic research methods, could facilitate the study of genomic structure and be adapted for chromosome studies of other animal species.  相似文献   

10.
Aquatic weeds are one of the major unconventional feed ingredients tested for aquafeed formulation. Tannin content in the water lettuce, Pistia, has been quantified (26.67 mg g−1; dry weight) and graded levels of which (12.5–200 μg) have been incorporated in the reaction mixtures to evaluate any change in the in vitro activity of the principal digestive enzymes from the three Indian major carps (IMC), namely rohu (Labeo rohita), catla (Catla catla) and mrigala (Cirrhinus mrigala). Result of the experiment revealed that the Pistia tannin (PT) significantly inhibit/lower the activities of the digestive enzymes from three IMCs in a dose-dependant manner, even at very low concentration. Significant variation in the reduction of the enzyme activities was noticed between the three fish species, as well as between the three enzymes studied. Among the three species studied, digestive enzymes from L. rohita were found to be the most sensitive to the PT, whereas enzymes from C. catla were found to be comparatively least affected. On the other hand, protease and lipase activities were comparatively more affected than the amylase activity. The results of the study suggest that more stress should be given on the elimination of tannin while incorporating feed ingredients of plant origin in fish diets.  相似文献   

11.
A polyculture experiment with the large carp rohu, Labeo rohita (Hamilton), catla, Catla catla (Hamilton) and either mrigal, Cirrhinus mrigala (Hamilton) or common carp, Cyprinus carpio (L.) (as cash crop fish), and the small indigenous fish punti, Puntius sophore (Hamilton) (as food for the small‐scale farmer family) was carried out at the Field Laboratory of the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. The main objective was to compare polycultures of large carp in which the bottom feeder is either the native mrigal or the exotic common carp. Secondary objectives were to assess the effects of adding the small indigenous species punti to polycultures of large carp, and to compare the effects of mrigal and common carp on punti production and reproduction. It was found that (i) common carp damaged embankments, had no effect on catla, improved rohu performance by 50% and total fish production by 20%; (ii) punti addition did not affect rohu, catla and total yield, improved mrigal performance by 50%, and decreased common carp performance by 20%; and (iii) punti was not affected either by common carp or by mrigal. However, its performance was not satisfactory, probably owing to frequent netting, which might have hindered growth and breeding. In spite of the embankment damage caused by common carp, this bottom feeder seems to be more promising than mrigal, because it leads to higher fish production. The addition of punti to the large carp polyculture is a viable proposition, as it does not reduce cash crop production, and might be a good food source for a small‐scale farmer's family.  相似文献   

12.
A modified SDS-PAGE system has been employed to resolve polymorphic myosin heavy chain (MyHC) isoforms in different muscle types of three freshwater teleosts displaying different modes of respiration, adaptive features and life styles. Investigated species include accessory air-breather Channa punctata along with exclusive aquatic breather major carps Labeo rohita and Catla catla. All the selected species show specificity and expressivity of at least three MyHC isoforms, one each in red, head and pectoral muscles. Chymotryptic peptide maps unambiguously support substructural individuality of each MyHC isoforms with the type-specific dispersal of chymotryptic cleavage sites. Specific Ca2+- and Mg2+-ATPase activities of natural actomyosin (NAM) of lateral line red muscle of C. punctata were low and less sensitive to pH, but sensitive to KCl concentrations between 0.05 and 0.15 M. In comparison, the specific enzymatic activities of NAM of red muscle from the carps (L. rohita and C. catla) were substantially high with prominent peaks at pH 7.5 and near insensitivity to 0.05–0.15 M KCl, while C. punctata had shown a different response at these molarities. Thus, the data favor a correlation between breathing modes and life style and the differences in pH or ionic strength sensitivities of ATPases. Unique profiles of peptide maps and the dispersal patterns of hydrophobic residues (cleavage sites of chymotrypsin) in MyHC of different muscle types further reflect individuality of their evolutionary histories.  相似文献   

13.
Growth performances of advanced fry of Indian major carps. Labeo rohita (Hamilton). Catla catla (Hamilton) and Cirrhinus mrigala (Hamilton), were examined in simulated pond systems using monthly application of the following fertilizers in six treatments: Mussoorie rockphosphate (MPR) in low (100 kg P2O5 ha?1) and high doses (200 kg P2O5 ha?1), single superphosphate (SSP) (50 kg P2O5 ha?1), single superphosphate mixed with rockphosphate (1:1 by weight: 50 kg P2O5), compost (C) (water hyacinth and cattle manure) and composted rockphosphate (MPR-C). The amount of available P in surface sediment of MPR-C did not differ from that in either the SSP or the MPR + SSP treatment, whereas the total and available N contents differed greatly. There was an inverse relationship between the time to reach phosphate peak of water and the retention time of fertilizer in surface sediments. It is evident that total P of surface sediment was the most significant contributor to fish growth in exclusively MPR treatments, whereas total P and total N of surface sediments are involved in the MPR treatments in combination with compost or SSP.  相似文献   

14.
Probotic selection could be detrimental for the success of aquaculture practices. While, in most of the cases emphasis is given to the production of antibacterial compounds, and in vitro inhibitory activities of a bacterium for choosing a probiotic strain, however, other criteria like non‐antibiotic resistant, non‐haemolytic nature and non‐pathogenic potentiality of probiotic strain are often overlooked. We have screened gastrointestinal bacteria isolated from all the three species of Indian major carps viz., Labeo rohita, Catla catla and Cirrhinus mrigala for a suitable probiotic. Bacteria like Aeromonas (Aeromonas species, A. hydrophila), Micrococcus, Corynebacterium, Plesiomonas, Bacillus (Bacillus species, B. subtilis) and Pseudomonas (Pseudomonas species, P. aeruginosa) exhibited antibacterial activities against different pathogens. Among these, B. subtilis was found to grow at a wider range of temperatures, pH and salt concentrations and was non‐haemolytic, non‐antibiotic resistant, non‐pathogenic and inhibited all the tested primary and secondary pathogens. Dietary supplementation of the bacteria at 108 CFU g?1 feed also enhanced the growth of Indian major carp, L. rohita. Based upon these characteristics, we aimed to develop a suitable probiotic for aquaculture practices.  相似文献   

15.
An evaluation of the apparent dry matter, crude protein, fat, carbohydrate and energy digestibility coefficients in nine raw and two steam-cooked agrobased by-product pelleted feeds (ratio of reference:test ingredient= 70:30) containing 30-32% crude protein, 3.9-4.3 kcal g?1 energy and 1% chromic oxide was carried out in three species of Indian major carp fingerlings, Catla catla (Hamilton) (length= 4-6cm; weight= 1.5-2.5g), Labeo rohita (Hamilton) (length= 3.9-6.2cm; weight= 1.5-2.7g) and Cirrhinus mrigala (Hamilton) (length= 4.1-6.0cm; weight= 1.6-2.8g). Each dietary treatment had three replicates of 25 fish each. The experimental trial was conducted in 70-L high-density polyvinyl flow-through (1-1.5 Lmin?1) indoor circular troughs (water volume= 55 L). Fish were fed to apparent satiation, twice daily at 0800 and 1600 h. Faecal samples were collected by siphoning daily at 0700 and 1500 h. The results indicated that apparent dry matter, nutrient (protein, fat and carbohydrate) and energy digestibility coefficients varied significantly within each test species (P < 0.05) for the feedstuffs tested. However, with few exceptions, digestibility for an individual ingredient varied insignificantly (P > 0.05) among the three fish species. The present study clearly indicated that feedstuffs rich in carbohydrate and energy are effectively utilized by the fish. The variations observed in dry matter, nutrient and energy digestibility coefficients in these species seemed related to the type and nutrient composition of the feedstuffs. Feedstuffs and test diets with higher levels of fibre and ash showed significantly (P < 0.05) low values for dry matter, nutrient and energy digestibility. Steam-cooking of yellow corn and potato significantly (P<0.05) improved the digestibility coefficients. The findings of the present study are important for the development of low-cost balanced rations which incorporate locally available agro-based by-products for the polyculture of the three species of Indian major carp.  相似文献   

16.
Three indigenous carp species, Catla catla (Ham.), Labeo rohita (Ham.) and Cirrhinus mrigala (Ham.), and two exotic carp species, Cyprinus carpio L. and Hypophthalmichthys molitrix (Val.), were stocked together at a total density of 6000 fish ha-1 in the ratio of 11:22:14:14:11, respectively. Three pelleted feeds, maize gluten (MG), cotton seed (CS) and fish meal (FM), were fed at 5% of the body weight of fish per day. The crude protein content of each feed was different. Growth rates for exotic species were significantly higher (P < 0.05) on all feeds than growth of indigenous species. The best growth for both exotic and indigenous carps was achieved on fish meal. Supplemental feeds made from locally available materials can enhance fish culture in Pakistan.  相似文献   

17.
This experiment was carried out in the framework of a project to develop a viable fish polyculture technology under Bangladeshi conditions that allows simultaneous fish production of small indigenous species for the farmers' family consumption and of large carp species as a cash crop. The objectives of this experiment were to assess the effects on fish performance and on the environment of adding 20% large fish to the basic ‘cash crop’ carp–small fish polyculture consisting of 10 000 fish ha?1 of the large carp rohu Labeo rohita (Hamilton), catla Catla catla (Hamilton) and common carp Cyprinus carpio (L.) at a species ratio 1:1:1, and 15 000 fish ha?1 of each small indigenous fish punti Puntius sophore (Hamilton) and mola Amblypharyngodon mola (Hamilton) (control). The treatment ponds were stocked with additional 2000 large fish ha?1, either all rohu, or all catla, or all common carp, or half rohu and half common carp, or half catla and half common carp. The results obtained and the analysis of interactions through the food web that affect food resource availability of the different fish species and account for the trends and differences observed among treatments confirm the positive effect of common carp on rohu reported in previous experiments and show that a 20% increase in large carps stocking neither affect the survival of the large carps nor reduce harvesting biomass of the small fish for the farmer's family consumption. Increased stocking densities of each large carp species did not significantly reduce its own harvesting weight and mean growth rate, while significantly increased rohu and catla (but not common carp) harvesting biomass and yield. The complex relations between species led to inter‐ and intraspecific competition, which in some treatments increased growth or yield of one species and in other treatments of other species, so that the gains on one species and the losses on the other led to no significant total harvested biomass differences between treatments. Yet, the results herein reported may help farmers to select their species stocking ratios. Thus, if the main target of the farmer is rohu, then a stocking density increase of 10% common carp and 10% rohu would improve rohu growth rate (due to common carp) and result in 50% higher rohu harvesting biomass and yield. If the main target of the farmer is catla, then a 20% increase in catla stocking density would lead to 20% higher catla harvesting biomass.  相似文献   

18.
Glycogen content and metabolic enzyme activities viz. lactate dehydrogenase (LDH), malate dehydrogenase (MDH), aspartate amino transferase (AST) and alanine amino transferase (ALT) in Indian major carps, Labeo rohita, Catla catla and Cirrhinus mrigala, were investigated after a 6 h transportation trial to compare the species‐specific variation and the effect of increased packing density on the metabolism. Fish (45±5 mm, 0.5±0.1 g) were packed in three densities (100, 150 and 200 L?1) for the experiment, and 12 specimens of each species were randomly sampled from all the treatments at the end of transportation. The glycogen content of L. rohita ingerlings decreased significantly (P<0.05) with increasing packing density. The activities of enzymes LDH, MDH, AST and ALT showed a rising trend with increasing packing density in all the three species. Species‐specific differences were observed in various tested parameters at the lowest packing density (100 fry L?1). Alanine amino transferase and LDH activities were significantly (P<0.05) lower in C. mrigala as compared with the other two species. However, glycogen reserves and MDH activity were not significantly different (P>0.05) among the species. The present study reveals that the optimum packing density for Indian major carp fry (100 fry L?1) for transportation up to 6 h and metabolic regimes are species specific during transportation.  相似文献   

19.
The innate immune system, particularly the external body surface, plays a frontier role in protecting fish from relevant infections. The present study is aimed at understanding and comparing the mucosal immunity of Indian major carp, that is, Labeo rohita, Catla catla and Cirrhinus mrigala, by evaluating different immune parameters such as protein content, lysozyme, myeloperoxidase, proteases and alkaline phosphatase activity in the skin mucus. The protein content of mucus sample of these species was compared, and the highest protein content was found in C. catla among the three Indian major carp species. The levels of proteases (40 ± 0.211 units/ml) and myeloperoxidase OD450 nm (1.525 ± 0.108) were found to be highest in the skin mucus of C. catla. However, lysozyme levels were highest in the skin mucus of L. rohita (10.95 ± 0.330 μg/ml), and C. mrigala had the highest alkaline phosphatase activity (30.74111 ± 0.680 U/l). Besides the enzyme activities, the epidermal mucus samples of three Indian major carp species were also tested and compared for the antibacterial activity against seven bacterial strains. Skin mucus of C. catla showed highest antibacterial activity among the three Indian major carps against all the seven bacterial strain, except that Micrococcus lysodeikticus and Vibrio cholerae, however, showed highest activity against mucus of C. mrigala. Also, the epidermal mucus from all the three species successfully agglutinated three freshwater pathogens, viz. Aeromonas hydrophila, Pseudomonas fluorescens and Edwardsiella tarda, with agglutination titres being the highest for Labeo rohita for all the pathogens. The epidermal mucus samples from the Indian major carp species showed haemagglutination activity and successfully lysed human, chicken and goat RBCs showing highest activity in C. catla. These results provide information for a better understanding of the role of epidermal mucus and its components in the innate immune system of Indian major carps.  相似文献   

20.
Antinutritional effects of phytic acid (myoinositol hexaphosphate, IP6) on growth and digestibility in fish have been reported. However, specific effect of IP6 on the digestive enzymes in fish has not been addressed. In this study, inhibitory effect of synthetic IP6 (Phytic acid sodium salt, 90% purity) on the activity of the digestive protease and α‐amylase in rohu, Labeo rohita; catla, Catla catla; and mrigal, Cirrhinus mrigala has been investigated in vitro. Graded levels (12.5, 25, 50, 100, 150, and 200 µg/mL) of IP6 were added to the reaction mixtures containing enzyme extracts and substrate solution in triplicate to detect any change in enzyme activity. Results of the experiment revealed that IP6 significantly inhibit/lower activities of the digestive enzymes in a dose‐dependent manner, as evident from the regression equations (F values significant at P < 0.001 level). Apparently, irrespective of the fish species studied IP6‐induced inhibition of α‐amylase activity was greater than protease activity. Among the three fish species studied, C. mrigala appeared to be more sensitive to IP6 for both α‐amylase and protease activity. Enzyme activity was least affected in C. catla. Results of the study might raise concern while incorporating IP6 rich plant‐derived feed ingredients in aqua feed preparation.  相似文献   

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