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以泰国无筋地豆为试材,在温室盆栽的条件下,分别单接种丛枝菌根真菌(AM)、单接种根瘤菌(RH)、双接种丛枝菌根真菌和根瘤菌(AM+RH),以不接种为对照(CK),研究不同接种方式对菜豆植株生长、结瘤、单株总荚质量、AM菌根侵染率、植株氮磷含量及吸收量、单株黄叶率等的影响。结果表明:双接种AM+RH能显著促进菜豆植株的生长,植株总干质量比单接种AM、单接种RH和CK分别增加34.3%、82.3%和57.9%;双接种AM+RH可提高菜豆单株总荚质量,促进菜豆植株对氮、磷元素的吸收,全株吸磷量比单接AM、单接RH和CK分别增加42.1%、314.8%和325.4%,全株吸氮量比单接种AM、单接种RH和CK分别增加36.8%、45.1%和43.4%;AM真菌与RH具有协同促进的作用,且AM真菌能有效提高菜豆的抗性,减少叶片变黄的比率,减轻了病害为害程度。 相似文献
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非灭菌土接种AM真菌对油蒿抗旱性的影响 总被引：1，自引：0，他引：1
利用盆栽试验在正常水分和干旱胁迫条件下研究了非灭菌土接种AM真菌摩西球囊霉(Glomus mosseae)和土著AM真菌(Indigenous arbuscular mycorrhizal fungi)对油蒿(Artemisia ordosica)生长及抗旱性的影响。结果表明:干旱胁迫显著抑制了AM真菌对油蒿的侵染。无论在正常水分还是干旱胁迫条件下,接种AM真菌都增加了植株的分枝数、地上部鲜重和干重、地下部鲜重和干重,但没有明显提高株高、茎粗和改善组织水分状况;与未接种相比,干旱胁迫下接种土著AM真菌显著提高了根系氮、磷含量,提高了叶绿素、可溶性蛋白含量,增强了保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性并降低了丙二醛的含量,因此增强了植物的抗旱性。 相似文献
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丛枝菌根真菌对水分胁迫下枇杷实生苗生长和养分吸收的影响 总被引：4，自引：0，他引：4
以‘早钟6号’枇杷（Eriobotrya japonica Lindl.‘Zaozhong 6’）实生苗为试材,研究了3种水分梯度（正常供水、轻度水分胁迫和重度水分胁迫）下,分别接种3种丛枝菌根（arbuscular mycorrhiza,AM）真菌（光壁无梗囊霉Acaulospora laevis、摩西球囊霉Glomus mosseae和苏格兰球囊霉Glomus caledonium）对实生苗生长和养分吸收的影响。结果表明,接种AM真菌的植株具有更高的地上部和地下部干质量;3种AM真菌均可增加枇杷苗氮、钾、磷、钙、镁、铜的吸收。说明接种AM真菌增强了枇杷苗的养分吸收能力,提高了其抗旱性,促进了枇杷苗生长。在3种AM真菌中苏格兰球囊霉是枇杷苗的优势菌种。 相似文献
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《中国瓜菜》2021,(7)
为了探究生物质炭和丛枝菌根(arbuscular mycorrhizal,AM)真菌对连作甜瓜生长的影响,试验设接种或不接种AM真菌并施用0、1%、2%和4%的生物质炭,共8个处理,测定甜瓜的生长生理指标和土壤理化性质。结果表明,单施用或单接种处理均能提高甜瓜的生长指标。而接种AM真菌并施用生物质炭,能显著增加土壤中细菌和放线菌的数量,提高土壤酶活性,提高甜瓜叶片的净光合速率,从而促进甜瓜植株的生长发育,其中以接种AM真菌加施用2%～4%生物质炭的效果最好,地上部鲜质量分别比对照增加了55.11%和66.09%;地下部鲜质量分别比对照增加了32.98%和43.62%;根长分别比对照增加了57.73%和78.78%。综上所述,接种AM真菌和/或施用生物质炭均可以促进甜瓜的生长并改善土壤环境,接种AM真菌并配合施用2%～4%生物质炭最为经济合理。 相似文献
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丛枝菌根真菌与葡萄南方根结线虫的相互作用及其对寄主的影响 总被引：9，自引：0，他引：9
在温室盆栽条件下对‘巨峰’葡萄扦插苗接种丛枝菌根(AM) 真菌(Glomus versiforme Berch、Gigaspora margarita Becker & Hall 及Glomus mosseae Nicolson & Gedermann) 和南方根结线虫(Meloidogyne incognita) 。结果表明, 供试AM真菌能不同程度地抑制南方根结线虫病害的发生发展, 降低根围土壤中二龄幼虫数量、卵块直径、平均每个卵块的卵量及平均每克葡萄根系上的卵量, 先接种AM真菌后接种南方根结线虫的处理比同时接种二者抑制线虫病害的效果更好。接种AM真菌可提高葡萄植株光合速率, 增加植株高度、茎粗、叶面积、地上部干样质量和地下部干样质量, 这在一定程度上补偿了由于线虫所造成的损害。此外, 接种南方根结线虫降低了G. mosseae 的根内泡囊和入侵点数量、G. versiforme 及Gi . margarita的侵染率;但增加了G. versiforme 及Gi . margarita 的根内泡囊数及入侵点数。因此, 对每一种AM真菌—植物—线虫组合关系不可能作出统一的概括。 相似文献
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AM真菌对连作西瓜生长及其枯萎病的影响 总被引：1，自引：0，他引：1
以中抗枯萎病西瓜品种“京欣1号”为试材,在温室栽培条件下,针对3种不同连作年限(3、7、12 a)的土壤,研究了接种丛枝菌根(AM)真菌对连作西瓜生长和枯萎病的影响.结果表明:接种AM真菌能显著提高西瓜根系菌根侵染率,且随着连作年限的增加,AM真菌侵染率逐渐降低;同时接种AM真菌均能提高不同连作年限处理西瓜植株的生物量,其中对连作3a的处理增加效果最显著;虽然AM真菌不能防止连作西瓜枯萎病害的发生,但对其具有一定的防治效果,并且连作年限越短,AM真菌的防治效果越好. 相似文献
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AIM: To study the role of c-myc oncogene in L6565 leukemia oncogenesis and the effects of therapy by inhibition of its expression with antisense c-myc. METHODS: A recombinant retroviral vector containing antisense c-myc of the murine (pGNCas) was constructed and then transfected into PA317 cells by the method of calcium phosphate precipitation. L6565 clone cells were infected with retrovirus particles. Stable integretion of antisense c-myc was shown by PCR. The change of the malignance and phenotype of L6565as were detected by the examination of the growth, morphology, cells cycle, agar assay and expression of c-myc. RESULTS: The shape of most L6565as cells became spherical. The growth of L6565as was inhibited compared to control cells. The analysis of cells cycle: L6565as cells were arrest in G0/G1 phase, decreased in S phase. The ability of L6565as cells to form colony in soft agarose was significantly suppressed. c-myc in L6565as cells was lowly expressed. CONCLUSION: (1)c-myc plays a critical role in L6565 leukemia oncogenesis; (2)Inhibition of expression of c-myc makes partly reversion of malignant phenotype of L6565 murine leukemia clone cells. 相似文献
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核桃壳与木屑栽培平菇的营养成分对比分析 总被引：1，自引：0，他引：1
测定了以核桃壳为基质(受专利保护)栽培平菇的主要营养成分和矿质元素,通过与木屑为基质栽培平菇的营养成分和矿质元素比较,结果发现:核桃壳种植的平菇粗纤维和多糖的含量明显高于木屑栽培的平菇,粗纤维和多糖分别相差13.8%和3.64%;蛋白质含量略低,相差4.5%;核桃壳组平菇矿质元素含量较高,尤其是铁和锌的含量,核桃壳组平菇铁和锌分别高于木屑组平菇32.3mg.kg-1和36.3mg.kg-1。 相似文献
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以"青大1号"紫花苜蓿(Medicago sativa L.)新品系青干草为试材,采用超声辅助酶解法提取总皂苷,对所得总皂苷提取液用石油醚、正丁醇进行2项萃取,紫外分光光度法测定总皂苷的含量。以单因素试验设计分别考察固液比(A)、超声时间(B)、超声温度(C)、超声功率(D)4个因素对总皂苷的粗总皂苷提取率的影响。以单因素试验结果为依据,采用4因素(固液比、超声时间、超声温度、超声功率)3水平正交实验设计进行提取工艺优化,以开发新的紫花苜蓿总皂苷资源。结果表明:超声法提取总皂苷最优工艺条件为A3B1C1D2,即固液比为1∶30g/mL、超声时间20min,超声温度20℃、超声功率360W。 相似文献
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AIM: To investigate the difference between immune-related pain induced by antigen-special complex and inflammatory pain induced by formalin, and to observe the differential expression of p38 mitogen-activated protein kinase in spinal cord. METHODS: Thirty adult health SD rats were randomly divided into control group, formalin group and immune complex group (10 rats in each group). After the baseline tests were finished, 5 rats in each group underwent intrathecal administration of p38 MAPK inhibitor SB203580. The right hindpaw of the rats were injected with PBS, formalin or rat IgG immune complex. The thickness of hindpaw and pain behaviors were observed at time points of 0 min, 30 min, 1 h, 2 h, 4 h, 8 h and 12 h after injection. The expression levels of total and activated p38 MAPK in spinal cord were determined by Western blotting analysis. RESULTS: The rats in formalin group showed significant nociceptive behaviors immediately, such as licking foot, and limping with highly swollen foot which could touch the ground. The pain threshold was decreased rapidly 30 min after injection and alleviated after then. The pain threshold of the rats in immune complex group obviously decreased 4 h after injection without red swollen hindpaw. The expression of activated p-p38 MAPK in spinal cord in formalin group was significantly higher than that in immune complex group and control group (P<0.01). No statistic difference of p-p38 expression between immune complex group and control group, also no significant effects of SB203580 on pain behaviors in immune complex group were observed. CONCLUSION: Activated p38 MAPK contributes to the pathogenesis of inflammatory pain, but not to the pathogenesis of immune-related pain. The mechanism of immune-related pain is different from inflammatory pain induced by formalin. 相似文献
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N.A. Salas F.J. Molina-Corral G.A. González-Aguilar A. Otero D.R. Sepulveda G.I. Olivas 《Scientia Horticulturae》2011
The effect of AVG application on the quality and volatile production of ‘Golden Delicious’ apples, grown in Chihuahua Mexico, was evaluated. AVG (125 g AVG/ha) was applied on apple trees four weeks before harvesting. Apples were harvested 176 days after full bloom and stored at 8 °C for 35 d. Quality parameters and volatile compounds were periodically evaluated. AVG application retarded color changes, and preserved acidity of apples during storage. Ethylene synthesis was also delayed by AVG. Control apples presented higher total soluble solids content than AVG-treated apples at the end of cold storage. Volatile production was affected by AVG application, especially in the case of esters and alcohols, which presented 59% and 33% lower values in AVG-treated apples at the end of storage. A higher concentration of aldehydes was observed at harvest time in AVG-treated apples; however, no difference was observed between treatments during storage. Higher correlations between ethylene production and aldehydes and alcohols were found in control apples (0.98, and 0.98, respectively), than in AVG-treated apples (0.6 and 0.90, respectively). AVG application on ‘Golden Delicious’ apples conferred a variety of benefits, such as delaying maturation, maintaining color, and reducing changes in acidity and total soluble solids. However, AVG treatment negatively affects the production of some volatile compounds. 相似文献
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SHAN Zhi-xin LIN Qiu-xiong YU Xi-yong DENG Chun-yu YANG Min FU Yong-heng TAN Hong-hong ZHENG Meng LIN Shu-guang 《园艺学报》2006,22(7):1263-1267
AIM:To study the potential of using antisense RNA mediated by expression vector to suppress MIF expression. METHODS:MIF gene was sub-cloned into plasmid pcDNA3 to construct MIF antisense RNA expression vector, pcDNA3-antiMIF, which was identified by restriction enzyme digestion and DNA sequencing. By using lipofectamine 2000, plasmid pcDNA3 and pcDNA3-antiMIF were transfected into MIF expression cells, 293-MIF, separately. 60 h later, the 293-MIF cells were collected and used to determine the MIF mRNA expression by real-time quantitive PCR. Plasmid pcDNA3-antiMIF was transformed into HUVECs, named HUVECs-antiMIF, to express MIF antisense RNA. HUVECs-antiMIF was screened by sulfate G418 and identified by PCR and RT-PCR analysis. Then the MIF expression vector, pSecTag-MIF, was transfected into HUVECs-antiMIF, and the MIF mRNA expression in HUVECs-antiMIF was determined by quantitative PCR. RESULTS:Restriction enzyme digestion and DNA sequencing analysis showed that MIF antisense RNA expression vector, pcDNA3-antiMIF, was constructed correctly. The results of quantitative PCR showed that MIF mRNA expression was suppressed by MIF antisense RNA at level about 32% (P<0.05) in 293-MIF cells. The HUVECs-antiMIF, which expressed MIF antisense RNA, was obtained and identified by PCR and RT-PCR assay. The results of quantitative PCR revealed that MIF mRNA expression was also down-regulated by about 40% (P<0.05) in HUVECs-antiMIF cells. CONCLUSION:MIF expression was suppressed efficiently by MIF antisense RNA mediated by expression vector, and the HUVECs-antiMIF was established to express MIF antisense RNA. 相似文献
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AIM: To investigate the implantation of matrigel carrying human adipose-derived mesenchymal stem cells to enhance the cell survival and the improvement of the ventricular functions in infarct heart.METHODS: Human adipose-derived mesenchymal stem cells (ADMSCs) were isolated and cultured from adult adipose tissue. SD rats with one-week-old myocardial infarction were randomly received the following 4 treatments: injection of PBS, matrigel, PBS+ADMSCs or matrigel+ADMSCs, respectively. Labeled ADMSCs either in matrigel or in saline were injected into the border area of ischemia. The controls received the injection of matrigel or saline only. Four weeks after injection, the heart functions were determined by echocardiography. The densities of the micro-vessels within the infarct area were also measured.RESULTS: Four weeks after implantation of ADMSCs, the cell graft size, the heart functions and the micro-vessel densities within the infarct area improved in matrigel+ADMSCs group as compared to other groups.CONCLUSION: The co-injection of ADMSCs with matrigel enhances the graft survival, increases the density of the micro-vessels in the myocardium and improves the cardiac functions. 相似文献
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Hong-Wei Zhou Ruth Ben-Arie Li Dong Shaul Burd Asya Weksler 《The Journal of Horticultural Science and Biotechnology》2013,88(5):620-628
SUMMARYPeaches (Prunus persica ‘Hermoza’) were ripened at 208>C after harvest and either stored at 08C for four weeks (control) to induce chilling injury or given intermittent warming (IW) on the twelfth day of storage (28C for 24.h) to alleviate chilling injury (CI). Continuously stored fruit from control developed wo olliness, a CI disorder, during ripening at 208C after cold storage while only a small percentage of IW fruit developed woolliness. CI fruit produced less ethylene during ripening after storage, and this inhibited ethylene production was closely tied with woolliness development. The IW treatment caused enhanced ethylene production in the fruit when returned to 08C and the ethylene remained higher than control fruit until the end of the storage period. IW also induced the messages for 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) and 1-aminocyclopropane-1-carboxylic acid synthase (ACS) in the ethylene synthesis pathway. IW also elevated the mRNA of the cell wall degrading enzymes polygalacturonase (PG) and endo-1,4-gluconase (EGase). The mRNAs of the cell wall enzymes and the enzymes in the ethylene synthesis pathway remained higher even after 5.d at 08 C following the IW treatment. It is suggested that IW maintained the fruit tissue capacity to ripen normally by preventing inhibition of the ethylene synthesis pathway which occurred in the control fruit after extended storage. 相似文献