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1.
Abstract

Four European (Vitis vinifera L.) winegrape cvs., ‘Semillon’, ‘Pinot Noir,’ ‘Chardonnay’, and ‘Cabernet Sauvignon’, and one American (Vitis aestivalis Michx.) winegrape cv. ‘Cynthiana’, were subjected to three temperature regimes in growth chambers set at 20/15°C, 30/ 25°C, or 40/35°C, for 16/8 hr day/night to determine the influence of temperatures on vine growth and development. In general, the best temperature for shoot and root growth 28 days after temperature treatments was 20/15°C for ‘Semillon’, ‘Cabernet Sauvignon’, and ‘Cynthiana’, and 30/25°C for ‘Pinot Noir’ and ‘Chardonnay’. Although 40/35°C reduced number of leaves, shoots, tendrils, and internodes, total leaf area (LA), and total shoot biomass of all the cultivars, the reduction was more pronounced in ‘Cynthiana’ than in the European cultivars. The average reduction in number of leaves at 40/35°C for the European cultivars was 47%, compared with 92% for ‘Cynthiana’. The two types of grapes adapted differently to high temperature. Shoot growth in the European cultivars continued under high temperature, whereas growth ceased in ‘Cynthiana’. Roots of ‘Cynthiana’, however, were less susceptible to the adverse effect of high temperatures than were the shoots. This study shows that the European cultivars were relatively more tolerant to high temperature than the American cultivar and they have a potential for production of wine in the climate of south central Kansas.  相似文献   

2.
The objective of the joint project between the two universities of Bonn and Sarajevo was to study the effects of climate change associated with warmer winter and spring temperatures on fruit tree phenology. Changes in flowering date of apple and cherry were correlated with local weather data obtained concomitantly on-site at four locations in the Balkan countries Slovenia, Serbia, B & H and at Bonn, Germany.Phenological data of flower opening (F1; BBCH 61) of apple cv. ‘Golden Delicious’ originated from 55 years at Cacak (44?°N, Serbia) and at Klein-Altendorf, University of Bonn (50?°N), cv. ‘Bobovec’ from the University of Ljubljana (46?°N, Slovenia) and cherry cv. ‘Burlat’ (35 years at Klein-Altendorf, 50?°N) and 20–30 years at Tuzla (44?°N, B & H) and Mostar (43?°N, Bosnia & Herzegovina; B & H).The annual precipitation showed a negligible change in the last 55 years of ±5% in the Balkan countries (e.?g. Cacak, 699?mm), but only ±1.8% at Klein-Altendorf (605?mm) with a slight shift to more winter rainfall.Regression analysis showed an increase in mean temperature of 1.7?°C (annual) and 1.45?°C for the vegetation period at Klein-Altendorf (last 55 years), Germany. In the evaluated Balkan stations, the greatest increase in annual air temperature between two climate phases (until 1987 versus from 1988 until now) was at Ljubljana (1.4?°C) and Cacak (1.2?°C) in contrast to the least increase (0.3?°C) at Tuzla (annual average 10?°C) and an intermediate value (0.9?°C) in the warmer Mostar (annual average 14.5?°C).This recent temperature increase advanced flower opening by 10–14 days for apple such as cv. ‘Golden Delicious’ at Cacak and 7–10 days (last 55 years) at Bonn and 4–5 days (last 35 years) for the early cherry cv. ‘Burlat’ at Klein-Altendorf/Bonn; at Mostar and Tuzla, the effect of climate change was greater with the early cherry cv. ‘Burlat’ (26 days) compared with the difference of the late cherry cultivar (16 days). Before recent climate change (1973–1987), cherry flower was advanced by 10 days in the warmer med climate at Mostar but delayed by 16 days in the cooler Tuzla. The results, i.?e. the flower advancement in the Continental climate but flower delay in the med climate, are discussed with respect to the risk of late frost and countermeasures to combat climate change effects.  相似文献   

3.
Early ripening sweet cherry cvs ‘Burlat’, ‘Earlise’, ‘Samba’, ‘Souvenir des Charmes’ and clone M, which are all characterised by large fruit size and dark red fruit, on GiSelA 5 rootstock were forced – by closing a polytunnel from 24 March to 20 April 2006 – at Klein-Altendorf research station of Bonn University. A portable gas heater was used for automated frost protection in April. An adjacent uncovered planting at the same spacing of 3.8?m?×?1.75?m served as control. Forcing resulted in a 16–18 days earlier flowering in April relative to the 12–16 days earlier harvest at the beginning of June, resulting in a 2–4 days longer fruit development and retarded ripening. PAR was reduced in the polytunnel by 40% on sunny and 30% on overcast days with a concurrent drop in the humidity to 30% on sunny and 40% on overcast days and nearly 100% relative humidity at night. Polytunnel air temperatures on sunny days were increased – during ventilation – by up to 6?°C relative to the outside, e.g. 33?°C versus 27?°C outside. Forced cherries saved one spray application against aphids compared to those outside. Fully-grown cherry leaves contained 40–60?mg chlorophyll/g FM sufficient for photosynthesis with commensurate chlorophyll contents when grown in the polytunnel compared with those outside, and were deficient in calcium (0.5% DM) and magnesium (0.2% DM), but with a surplus in both nitrogen (3.5% DM) and phosphorus (0.42% DM). The surplus leaf nitrogen was due to excessive nitrogen mineralization in the rich soil under the higher temperatures in the polytunnel which caused the vigorous vegetative growth despite the dwarfing rootstock. Great fluctuations in fertilisation – despite strong flowering and sufficient pollination with honey bees – of between 4–71% may be due to the combination of S-alleles of the cherry varieties, infrequent water supply, nutrient imbalance and microclimate. Trees of cv. ‘Burlat’, ‘Earlise’ and clone M in their fourth leaf yielded an average of 2.5–7.5?kg fruit with 15?kg/tree in one case. Fruit of forced cvs. ‘Burlat’, ‘Souvenir’ and ‘Samba’ were larger compared with those of cv. ‘Earlise’ and clone M, which were smaller as a result of increased fruit load on the tree than those from uncovered control trees. For all five cvs tested, except forced cv. ‘Earlise’, a fruit mass of between 10?g (28?mm ) and 12?g (30?mm) and a sugar content of 12–17% with sugar:acid ratios of 20–37:1 made their fruit suitable for marketing as superior or premium fruit at higher farm-gate prices of 4.5–8?€/kg. Fruits were sufficiently dark red or black and firm for short distance transport and rapid sale. An economic evaluation showed financial gains, when yields exceed 8?kg fruit per tree per year at a farm-gate price of 4.50?€/kg.  相似文献   

4.
Summary

To control the bolting of Japanese bunching onion (Allium fistulosum L.) photoperiodically, the effect of photoperiods before, during and after vernalization on flower initiation and development and the varietal differences were investigated using the two mid-season flowering cvs Kincho and Asagi-kujo, and a late-season flowering cv. Cho-etsu. A long-day photoperiod (LD, 16 h) given before vernalization inhibited flower initiation. Especially, the bolting rate of ‘Asagi-kujo’ decreased by about a half, compared with the short-day photoperiod (SD, 8 h). The interaction between the effect of night temperature (3°C, 7°C, 11°C or 15°C) and the effect of the photoperiod (SD and LD) during vernalization was also investigated. In ‘Kincho’, LD did not affect flower initiation at 3°C, but inhibited flower initiation at 7°C, 11°C and 15°C. In ‘Asagi-kujo’, flower initiation was significantly inhibited by LD under all temperature conditions. This inhibitory effect was stronger at 11°C and 15°C than at 3°C and 7°C. In ‘Cho- etsu’, LD significantly inhibited flower initiation at 3°C and 7°C, and flower initiation rarely occurred at 11°C and 15°C. In this study, generally, LD during vernalization inhibited flower initiation in all cultivars. Thus Japanese bunching onion required a short-day photoperiod in flower initiation, which was stronger in ‘Asagi-kujo’ and ‘Cho-etsu’ than in ‘Kincho’. From these results, we conclude that low temperature and a short-day photoperiod complementarily induce flower initiation in Japanese bunching onion. Varietal differences exist in the requirement of low temperature and a short-day photoperiod: the primary requirement in ‘Kincho’ is low temperature and that in ‘Asagi-kujo’ is a short-day. After flower initiation, the early stage of flower development is day-neutral, and after the floret formation stage, a long-day photoperiod promotes flower development and elongation of the seedstalk.  相似文献   

5.
Summary

Until recently, olive breeding programmes have been based exclusively on cultivated olive germplasm (Olea europaea subsp. europaea var. europaea), in contrast to breeding in other fruit crops where the use of wild relatives has been widely reported. In this study, ten agronomic traits were evaluated in two progenies derived from controlled crosses between the Spanish olive cultivar ‘Picual’ (female) and two wild (O. europaea subsp. europaea var. sylvestris) genotypes (males). The results of this evaluation were compared with the progenies of crosses between ‘Picual’ (female) and the three cultivars, ‘Sikitita’, ‘Meski’, and ‘Zaity’ (male). The two ‘Picual’ × wild genotype progenies had the highest mean values for vigour traits (i.e., tree height and trunk diameter). Progenies from both ‘Picual’ × wild genotype crossess also showed the highest percentages of trees (53.8% and 37.5%) with a short juvenile period, compared to the progenies from crosses between ‘Picual’ and each of the three cultivars ‘Sikitita’, ‘Meski’, and ‘Zaity’ (0%, 5%, and 3.6%, respectively). Significant differences were also observed between progenies in fruit traits as well as in oil contents and fruit weights. Progenies from both crosses with wild olive showed lower values for the fruit traits evaluated than the cultivated olive progenies. However, significant improvements were achieved compared to fruit traits in the wild parents. The implications of these results for the future use of wild germplasm in olive breeding programmes are discussed.  相似文献   

6.
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7.
Abstract

It is generally thought that olives (Olea europaea) require several days (over 80 days) of chilling temperatures (7.2°C) for flower induction; minimum nighttime temperatures of 2-4°C and maximum daytime temperature of 15.5-19°C are considered optimum for flower and fruit production. Environmental chamber studies were conducted on potted olive trees for the purpose of defining flowering conditions for ‘Arbequina’. We repeatedly observed that good flower and fruit production in ‘Arbequina’ can be achieved even when the plants are not subjected to “chilling” temperatures or any chilling criteria that had previously been described necessary for flower and fruit production in olives. This phenomenon could be of great practical value because the results obtained can be exploited to cultivate olives in subtropical climates (e.g., southern and coastal Texas) where typical “chilling” temperatures are not commonly observed for prolonged periods of time.  相似文献   

8.
Abstract

It is generally assumed that lack of flowering and fruiting in olives in subtropical climates of southern Texas (e.g. Weslaco Texas area; N 26.16° Latitude 97.96° Longitude) is due to fewer chilling days (<7.2°C) during winter than most olive growing areas of the world. However using controlled environmental chambers we have recently shown that flowering and fruiting in the ‘Arbequina’ cultivar of olives can be achieved without any chilling days. This raised the question of why olive trees don't flower in southern Texas where they do experience some chilling days. We hypothesize that the absence of flowering in olives growing in southern Texas and a similar climate elsewhere is due not to the lack of enough chilling days but most likely due to high temperatures during the day (≥°C) that inhibit flowering. To test our hypothesis we provided cooling to olive trees growing under Weslaco Texas climate by simple shading or by evaporative cooling. These treatments resulted in good flowering and fruiting in ‘Arbequina’ olives in Weslaco Texas after a typical winter period although normally olive trees in this subtropical climate remain vegetative even after winter months.  相似文献   

9.
Summary

‘Korona’, ‘Elsanta’, ‘Bounty’ and ‘Senga Sengana’ strawberry (Fragaria × ananassa Duch.) plants, were placed at constant temperatures of 9, 15 or 21°C and daylengths of 8 h (short day) or 24 h (long day). The plants were given different numbers of short-day (SD) cycles, and flowering and growth were studied. ‘Korona’ and ‘Elsanta’ were responsive to both short-day treatment and temperature, with optimum flowering at 15°C and 24 SD. ‘Bounty’ was more responsive to temperature, inducing flowers independently of the number of SD cycles at 9°C and 15°C. In ‘Senga Sengana’ flowering was induced independently of temperature and the number of SD cycles, indicating that it had a stronger dependence on other environmental effects. The effect of the number of short-day cycles and the temperature on vegetative growth variâtes such as the number of stolons and daughter plants, the length of flower trusses and petiole length were also studied.  相似文献   

10.
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11.
Summary

Shoot tips of two almond scion cultivars, ‘Ne Plus Ultra’ and ‘Nonpareil 15-1’, and one almond/peach hybrid rootstock were successfully cryopreserved using a one-step vitrification technique. Three week old in vitro cultures were cold-hardened at 4°C on the multiplication medium (Murashige and Skoog for ‘Ne Plus Ultra’ and the hybrid rootstock; Almehdi and Parfitt for ‘Nonpareil 15-1’) for three weeks. Shoot tips, 2–2.5 mm long, were excised and precultured for 1 d at 4°C on the same basal medium, without plant growth regulators, supplemented with 0.7 M sucrose. After the preculture, the shoot tips were incubated in vitrification solution at 25°C for 45 min for the almond scion cultivars and 60 min for the hybrid rootstock, and then stored under liquid nitrogen (LN) for at least 3 d. After rapid thawing at 30°C, the shoot tips were washed with the appropriate liquid basal medium containing 1.0 M sucrose and then cultured on the same basal medium, solidified with agar, but excluding NH4NO3 or (NH4)2SO4. Shoot regeneration was usually observed within 2–3 weeks. Survival after LN, recorded as the percentage of shoot tips that produced at least one new shoot four weeks after thawing, was 87.5, 60.0 and 72.5% for ‘Ne Plus Ultra’, ‘Nonpareil 15–1’ and the hybrid rootstock respectively. The one-step vitrification method is a promising simple technique for cryopreserving almond scion and rootstock shoot tips from in vitro cultures.  相似文献   

12.
Summary

Characterization and selection of olive clones for the production of olive oil is essential in Portugal because of its profitable exploitation. “Moura-Serpa” is the most important Portuguese region for the production of olive oil relying on three cultivars for oil quality. These are ‘Galega Vulgar’, ‘Cordovil de Serpa’ and ‘Verdeal Alentejana’. Therefore, it is of great importance to guarantee the varietal certification of the young trees and the establishment of new orchards. Random Amplified Polymorphic DNA (RAPD) technique was used to characterize these three cultivars. Analysis started using twenty primers that allowed us to distinguish the three cultivars and to select a reduced set of primers. The selected primers were used for inter- and intra-varietal analysis and for establishing a profiling system to assay genetic diversity in other olive cultivars. The method has the potential for use in varietal certification and breeding programmes that need to analyse a high number of samples.  相似文献   

13.
Abstract

1-Methylcyclopropene (1-MCP), an ethylene action inhibitor, was applied to ‘d’Anjou' pears (Pyrus communis L.) at 20°C between 2 and 5 days after harvest. Scald of ‘d’Anjou' pears was completely controlled by 1-MCP at a concentration between 0.05 and 0.3 µl l1 after a prolonged cold storage plus 7 days of exposure to an environment with or without 500 µl l1 ethylene at a temperature of 20°C or 25°C. 1-MCP inhibited the biosyntheses of α-farnesene and its oxidative products (conjugated trienes) and thus controlled scald. However, fruit treated with the above concentrations of 1-MCP did not ripen normally in an environment with or without ethylene. Ethylene production and fruit softening of 1-MCP-treated ‘d’Anjou' pears were inhibited during 7 and 15 days at 20°C. ‘d’Anjou' fruit treated with 0.01 and 0.02 µl l1 1-MCP ripened normally on day 7 at 20°C after 3 months of cold storage at ? 1°C, and ripened fruit did not develop any incidence of scald. Untreated fruit developed substantial scald. After 4 months of storage or longer, both untreated fruit and fruit treated with 0.01 and 0.02 µl l 1 1-MCP developed an unacceptable incidence of scald upon ripening. Thus, use of other scald control methods may be necessary in addition to treatment with a low dosage of 1-MCP to insure both normal ripening and scald control for d'Anjou pear fruit from the Mid-Columbia district.  相似文献   

14.
Summary

Photoperiod and temperature control of flowering in a number of perpetual-flowering or everbearing strawberry cultivars of widely varying pedigree has been studied in controlled environments. Flower bud initiation in the cultivars ‘Flamenco’, ‘Ridder’, ‘Rita’ and ‘Rondo’ was significantly advanced by long-day (LD) conditions at temperatures of 15°C and 21ºC; while, at 27ºC, flowering took place under LD conditions only. Some plants of the seed-propagated F1-hybrid ‘Elan’, raised at 21°C, also flowered under short-day (SD) conditions at 27°C, but reverted to the vegetative state after a few weeks when maintained under these conditions. When vegetative plants growing in SD at 27°C were transferred to LD conditions at the same temperature, they consistently initiated flower buds and started flowering after about 4 weeks. At such a high temperature, flowering could thus be turned on and off by switching between SD and LD conditions. This applied to all the cultivars studied. Also the cultivar ‘Everest’, which was tested only at 21°C, produced similar results. Night interruption for 2 h was effective in bringing about the LD response. At 9°C, flowering was substantially delayed, especially in ‘Flamenco’ and, at this temperature, flowering was unaffected by photoperiod. Runner formation was generally promoted by high temperature and SD conditions, but the photoperiodic effect varied between experiments. We conclude that everbearing strawberry cultivars, in general, whether of the older European-type or the modern Californian-type originating from crosses with selections of Fragaria virginiana ssp. glauca, are qualitative (obligatory) LD plants at high temperature (27°C), and quantitative LD plants at intermediate temperatures. Only at temperatures below 10°C are these cultivars day-neutral.  相似文献   

15.
Summary

Floral induction in tropical trees generally follows a check in vegetative growth. However, it is not easy to identify the environmental factors involved in flowering, which normally occurs during the dry season when temperatures are also often lower. The separate and combined effects of temperature and water supply on floral induction were investigated in ‘Hass’ avocado (Persea americana), ‘Lisbon’ lemon (Citrus limon). ‘Wai Chee’ litchi (Litchi chinensis) and ‘Sensation’ mango (Mangifera indica). Low temperatures (15°/10°C or 15°/10°C and 20°/15°C compared with 30°/25°C and 25°/20°C) generally decreased vegetative growth and induced flowering in well-watered avocado, litchi and mango. A pre-dawn leaf water potential (ψL) of ?1.7 to ?3.5 MPa compared with ?0.4 to ?0.7 MPa in control avocado and litchi, and a pre-dawn relative water content (R.W.C.) of 90-93% compared with 97% or above in control mango plants also reduced or eliminated vegetative growth, but did not induce flowering. Low temperatures (15°/10°C compared with 20°/5°C, 25°/20°C or 30°/25°C) and water stress (pre-dawn ψL of ?2.0 to ?3.5 MPa compared with ?0.7 to ?0.8 MPa in controls) reduced or eliminated vegetative growth in lemon. In contrast to the response in avocado, litchi and mango, flowering in lemon was very weak in the absence of water stress at 15°/10°C or outdoors in Brisbane in subtropical Australia (Lat. 28°S), and was greatest after a period of water stress. The number of flowers increased with the severity and duration of water stress (two, four or eight weeks) and was generally greater after constant rather than with cyclic water stress. In lemon and litchi, net photosynthesis declined with increasing water stress reaching zero with a midday ψL of ?3.5 to ?4.0 MPa. This decline in carbon assimilation appeared to be almost entirely due to stomatal closure. Despite the reduction in midday CO2 assimilation, starch concentration increased during water stress, especially in the branches, trunk and roots of lemon. Leaf starch was uniformly low. The number of flowers per tree in lemon was strongly correlated with starch in the branches (r2=77%, P<0.01) and roots (r2=74%, P<0.001). In litchi, starch was lower than in lemon roots and was not related to flowering.

In separate experiments to test the interaction between temperature and water supply, low day/night temperatures (23°/18° and 18°/15°C compared with 29°/25°C) reduced vegetative growth and induced flowering in avocado, litchi and mango. None of these species flowered at 29°/25°C or as a result of water stress (ψL of ?1.5 MPa compared with ?0.3 MPa for avocado and ?2.0 MPa compared with ?0.5 MPa for litchi, and R.W.C, of 90-93% compared with 95-96% in mango). In contrast, in lemon, flowering was very weak (<10 flowers per tree) in the absence of water stress (pre-dawn ψL of ?2.0 MPa compared with ?0.5 MPa) and was only heavy (>35 flowers per tree) after stressed trees were rewatered. There were slightly more flowers at 18°/15°C than at 23°/18° and 29°/25°C in control plants, but no effect of temperature in stressed plants. Starch concentration in the roots of avocado, lemon, litchi and mango was generally higher at 18°/15°C and 23°/18°C than at 29°/25°C. Water stress increased the starch concentration in the roots of lemon and litchi and decreased it in avocado. There was no effect in mango. There was a weak relation (r2=57%, P<0.05) between the number of flowers per tree in lemon and the concentration of starch in the roots. In contrast, there was no significant relationship between flowering and starch levels under the various temperature and water regimes in the other species. In another experiment, only vegetative growth in litchi and mango occurred at 30°/25°C and only flowering at 15°/10°C. Six weeks of water stress (pre-dawn ψL of ?2.5 MPa compared with ?1.0 MPa or higher in litchi, and R.W.C, of 90-93% compared with 95% or higher in mango) in a heated glasshouse (30°C days/20°C night minimum) before these temperature treatments did not induce flowering.

Temperatures below 25°C for avocado and below 20°C for litchi and mango are essential for flowering and cannot be replaced by water stress. The control of flowering in lemon over the range of day temperatures from 18°C to 30°C differed from that of the other species in being mainly determined by water stress. Flowering was generally weak in well-watered plants even with days at 18°C. Starch did not appear to control flowering.  相似文献   

16.
Summary

Self- and cross-incompatibility of the olive cultivars Frantoio, Manzanillo, Kalamata, Pendolino, and Picual were investigated using a 5 × 5 diallel matrix. Pistils were collected seven days after controlled pollinations on the day of flower opening, and pollen tubes were detected by fluorescence microscopy. Diallel analysis showed significant specific combining ability, general combining ability and reciprocal effects between cultivars for pollen tube growth in the pistil. ‘Frantoio’ was cross-compatible, as either a male or female parent, with each of the other cultivars, but showed a high degree of self-incompatibility. ‘Manzanillo’, ‘Kalamata’, ‘Pendolino’, and ‘Picual’ were crossincompatible, and all except for ‘Manzanillo’, were self-incompatible. It is concluded that ‘Frantoio’ is a good general polleniser for the other cultivars investigated. Pollen tube growth decreased in discrete steps from stigma to upper style, and from upper style to lower style, with the result that only one, and rarely more, pollen tube penetrated ovules. The sex ratio of flowers, and pollen viability using fluroescein diacetate staining and in vitro germination, were examined. ‘Frantoio’, ‘Manzanillo’ and ‘Pendolino’ had more than 80% perfect flowers, while ‘Kalamata’ and ‘Picual’ had less than 30%. ‘Frantoio’ had the highest pollen viability, ‘Kalamata’ and ‘Picual’ were intermediate, and ‘Manzanillo’ and ‘Pendolino’ the lowest. Pollen staining and both in vitro and in vivo germination provided the same male fertility rankings of cultivars.  相似文献   

17.
Summary

Moderate day/night temperatures (20/15° v. 15/10°C) increased vegetative growth and reduced flowering in the seven litchi cvs Tai So, Bengal, Souey Tung, Kwai May Pink, Kwai May Red, Salathiel and Wai Chee. At higher temperatures (25/20° and 30/25°C), vegetative growth was promoted further and flowering eliminated. Temperature also influenced the type of inflorescence formed. More leaves were formed on the panicles of trees growing at 20/15° than at 15/10°C. All terminal shoots on all cultivars produced panicles at 15/10°C. The relative order for the amount of flowering at 20/15°C was: ‘Wai Chee’>‘Salathiel’>‘Kwai May Pink’>‘Tai So’>‘Bengal’>‘Souey Tung’>‘Kwai May Red’. Cultivars which were vigorous at high temperatures produced fewer panicles at 20/15°C and fewer leafless panicles at 15/10°C. Only small differences were observed in the leaf water potential and the nutrient status of the shoots at different temperatures. Vigour and flowering of the cultivars in the glasshouse generally reflected field performance in subtropical Australia (Lat. 27°S). Low vigour could be useful for selecting litchi cultivars for good fruiting in environments with warm autumns and winters.  相似文献   

18.
Summary

Simple Sequence Repeat (SSR) and Sequence-Related Amplified Polymorphism (SRAP) molecular marker systems were used to analyse four commercially important pistachio rootstocks: two species of Pistacia atlantica (cv. ‘Standard Atlantica’), P. integerrima (cv. ‘Pioneer Gold’) and two interspecific hybrids of the same, ‘Pioneer Gold II’ (‘PGII’) and ‘University of California at Berkeley 1’ (‘UCB-1’). A total of 35 putative alleles were detected by 12 SSR primer pairs with an average of 2.9 alleles per locus. The number of putative alleles ranged from 2 to 5 in the pistachio rootstocks tested. The number of bands produced by the SRAP protocol was highly variable, ranging from 11 to 38, with an average of 25.2 per primer combination. Eight primer combinations resulted in 104 (51%) polymorphic markers in these samples. SSR and SRAP markers successfully identified all pistachio rootstocks tested from their unique fingerprints. Both SSR and SRAP molecular markers confirmed that the observed variation in ‘UCB-1’ rootstock is genetic.Thus, there will always be variation among ‘UCB-1’ hybrid seedling progeny due to the segregation of alleles when propagated by seed.We also found evidence of contaminating pollen other than from P. integerrima in some hybrid ‘UCB-1’ rootstock progeny produced by closed pollination. Only alleles from the cultivar ‘Standard Atlantica’ were observed in abnormal ‘UCB-1’ rootstock in the nursery. We found that the poor performance of the scion cv. ‘Kerman’ on ‘UCB-1’ rootstock was not due to ‘UCB-1' rootstocks displaying abnormal behaviour in the nursery. We have successfully developed two efficient marker systems for genome analyses in pistachio, which can be used for identification and management in pistachio rootstock production.  相似文献   

19.
Summary

Variations in the concentrations of cytokinins (CTKs) and abscisic acid (ABA) were studied in the pericarp of litchi (Litchi chinensis Sonn.) fruit using the large-fruited cv. ‘Erdanli’ (55.3 g per fruit) and the small-fruited cv. ‘Huaizhi’ (20.9 g per fruit), as well as large fruit (32.4 g) from early blooms and small (20.8 g) fruit from late blooms on the same inflorescences of cv. ‘Feizixiao’ from the same commercial orchard in Guangdong, China in 2000. ‘Erdanli’ had higher concentrations of CTKs than cv. ‘Huaizhi’ on three out of six sampling dates during fruit development, and lower concentrations of abscisic acid (ABA) from 40 d after anthesis. In cv. ‘Feizixiao’, fruit from early blooms had higher concentration of CTKs than fruit from late blooms at all sampling dates, and lower concentrations of ABA on three out of five sampling dates. Therefore, the former had a higher CTK:ABA ratio. These data suggest that a high CTK:ABA ratio favours fruit growth in litchi.  相似文献   

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