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1.
利用中性盐NaCl、碱性盐Na2CO3及混合盐碱(NaCl∶ Na2CO3=1∶1)设置3种盐碱胁迫处理,研究平欧杂种榛“辽榛3号”在不同类型、不同浓度的盐碱胁迫下生长指标及内源激素的变化,分析各内源激素与生长指标的相关关系.结果表明:3种盐碱胁迫对平欧杂种榛株高、新梢长度及叶片数净生长量、地上部生物量、总生物量抑制作用明显,且NaCl胁迫＞混合盐碱胁迫＞Na2CO3胁迫,而各处理根冠比显著增加;低浓度盐碱胁迫对地径有明显的增粗效应,在50、100 mmol·L-1 Na2CO3胁迫下,地下部生物量显著增加;此外,NaCl胁迫还显著降低了平欧杂种榛叶片含水量.3种胁迫条件下,平欧杂种榛叶片的ABA含量明显增加,且(GA+ IAA+ ZR)/ABA比值与对照相比均显著降低,同时发现Na2CO3胁迫下ABA积累更快,且与胁迫浓度无关,而NaCl胁迫下ABA含量与胁迫浓度显著正相关.相关分析表明:(GA+ IAA+ZR)/ABA比值与株高、新梢长度、新梢叶片数存在显著正相关,而与根冠比呈极显著的负相关.由上可知,平欧杂种榛在中性盐下的生长指标受抑制程度大于碱性盐和混合盐碱,不同盐碱胁迫下内源激素响应特征也存在差异,表明平欧杂种榛对Na2CO3的耐受力高于NaCl. 相似文献
2.
This paper studied the seasonal characteristics to resist the drought stress of Haloxylon persicum Bge. Ex Boiss. et Buhse photosynthetic shoots at habitat. The results showed that the predominant drought resistance factors
were varied at the different stage from growth to development. In the blooming season (from May 31 to June 29), endogenous
ABA contents were rare; stomatal conductance and photosynthesis intensity were the highest at the whole stage from growth
to development; soluble sugars contents had a decreasing trend and proline contents increased a little that made proline become
the predominant factor to resist the drought under this light water stress. In the hot summer (from June 29 to July 26), ABA
contents accumulated rapidly; stomatal conductance dropped to the lowest level of the growth and development; chlorophyll
was also decomposed; both soluble sugars and proline contents showed the trend of quickly accumulating, but the former was
faster than the latter. It was due to stomatal limitation and osmotic organic molecules accumulation that would affect the
photosynthetic shoots to resist severe drought stress. At the late period of the development (from Aug 9 to Aug 22), ABA rapidly
accumulated, its contents got to the highest level of whole life-span; stomatal conductance increased a little; proline and
soluble sugars contents changed little at high level; while the ratios of ABA to CTK content and ABA to IAA content got up
obviously, the effect to resist drought stress on high content ABA was inhibited by endogenous plant hormone CTK and IAA,
then the continuing accumulation of proline and soluble sugars would be prevented. Osmosis of organic molecules was the most
important factor to adjust leaves to severe water stress at this period.
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Translated from Scientia Silvae Sinicae, 2005, 41(5) [译自, 林业科学 2005, 41(5)] 相似文献
3.
白梭梭同化枝对干旱胁迫的生理生态响应 总被引：8，自引：1，他引：7
对白梭梭同化枝自然水分胁迫下的季节抗旱特征进行研究。结果表明:自然干旱胁迫条件下,不同生长发育时期同化枝的主导抗旱生理因子不同。5月31日至6月29日盛花期间,同化枝内源ABA含量甚微,气孔导度值与光合强度处于生长发育过程的最高水平,可溶性糖含量呈现下降趋势,而脯氨酸含量略有增加,植物通过渗透调节作用适应此阶段轻度干旱胁迫;6月29日至7月26日盛夏期间,ABA迅速积累,气孔导度值降为生长发育过程的最低值,叶绿素分解,可溶性糖与脯氨酸均呈现快速积累趋势,且可溶性糖积累强度大于脯氨酸;8月9日至8月22日同化枝生长发育后期,ABA急剧积累为生长发育过程的最高浓度,气孔导度值有所增大,脯氨酸和可溶性糖保持在高水平平稳变化。此阶段高浓度ABA调节植物生理过程适应干旱的效应受CTK、IAA两种内源激素的抑制,进而抑制脯氨酸和可溶性糖的继续积累。 相似文献
4.
Deuterium- and tritium-labeled gibberellins (GAs) were applied to stems of 3-year-old Eucalyptus globulus Labill. saplings and 9-month-old potted seedlings. Cambial region tissues surrounding the application point were collected 6, 24 or 48 h later. Twenty-four hours after application of 5 &mgr;g of [(2)H(2)]GA(20), 7% of the cambial region GA(20) pool, 7% of the GA(1) pool and 58% of the GA(29) pool were labeled with deuterium based on selected ion monitoring of purified extracts subjected to gas chromatography-mass spectrometry. The relatively low percent dilution of endogenous GAs by [(2)H(2)]GAs suggests that the exogenous application of [(2)H(2)]GA(20) did not result in substrate overloading, indicating that these conversions probably occur naturally within cambial region tissues. Extracts from similar cambial region tissues fed tritium-labeled GAs were sequentially fractionated by SiO(2) partition chromatography, C(18) reversed phase HPLC and N(CH(3))(2) HPLC. The radioactivity profiles indicated metabolism of GA(20) to GA(1) and GA(29), GA(1) conversion to GA(8), GA(4) to GA(34) and GA(9) to GA(51). Gibberellins GA(34), GA(51) and GA(29) are C-2beta-hydroxylated catabolites of low biological activity, whereas GA(1) and GA(4) are probably effectors of growth in the Eucalyptus stem and shoot. Evidence for C-13 hydroxylation of GA(4) to GA(1), GA(9) to GA(4) or GA(9) to GA(20) in the stem was inconclusive. Thus, although GA(4) and GA(9) are native to cambial region tissues, GA(1) is probably not produced from them in significant quantities. We conclude that the early C-13-hydroxylation pathway; i.e., conversion of GA(19) to GA(20) to GA(1), is the major pathway of GA(1) biosynthesis. 相似文献
5.
Lisheng Kong Patrick von Aderkas Stacey J. Owen Barry Jaquish Jack Woods Suzanne R. Abrams 《New Forests》2012,43(4):491-503
Stem-girdling treatments were applied in early spring to stimulate cone formation in two genotypes of interior Douglas-fir (Pseudotsuga menziesii var. glauca (Beissn.) Franco). After girdling treatments, male cone yield increased significantly in the next growing season. The increase was 14-fold in genotype 9137. In genotype 9550, more than 8,700 male cones were induced from each tree whereas no male cones were found in controls. Female cone yield was zero in controls and low for girdled trees in both genotypes. Multiple phytohormone-related compounds, including gibberellins (GAs), cytokinins, indole-3-acetic acid (IAA), abscisic acid (ABA) and their selected metabolites, were analyzed in developing long shoots after girdling treatments by high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC?CESI?CMS/MS) in multiple reaction monitoring mode. Concentrations of GA4 were slightly higher at week 2 following girdling treatment, whereas at week 8 lower GA4 concentrations were found in girdled samples. Stem girdling did not affect concentrations of IAA and major cytokinins, such as zeatin riboside and isopentenyl adenosine. Concentrations of ABA differed two-fold between the genotypes. Although girdling treatment did not cause differences in ABA concentrations, it generally resulted in higher concentrations of ABA glucose ester. Concentration increase of 7??-hydroxy ABA by girdling was only found in genotype 9550 at week 8. Girdling caused little change in concentrations of phaseic acid in both genotypes. 相似文献
6.
Cottonwoods occur in riparian areas where water table depth generally varies with the elevation of the adjacent river. Plant adaptation to the riparian zone requires the coordination of root elongation to maintain contact with the water table during the summer decline. We investigated the effects of rate of water decline on growth allocation and concentrations of endogenous gibberellins (GAs) in black cottonwood (Populus trichocarpa Torr. & A. Gray ex Hook.) saplings. Rhizopods were used to achieve water decline rates of 0, 2 and 4 cm day(-1). Root elongation approximately doubled in response to the 2 cm day(-1) treatment, whereas leaf area was reduced. A water decline rate of 4 cm day(-1) led to water stress, as evidenced by reduced growth, increased leaf diffusive resistance, decreased water potential, and leaf senescence and abscission. Endogenous GAs were extracted, purified and analyzed by gas chromatography-selected ion monitoring with internal [(2)H(2)]GA standards. Across the sampled plant organs, GAs were generally highest in shoot tips and sequentially lower in basal stems, root tips, leaves and upper roots; GAs were thus abundant in rapidly growing tissues. Of the GAs measured, GA(1) tended to predominate, followed sequentially by GA(3), GA(8), GA(19), GA(20), GA(29) and GA(4). There was little relationship between GA concentration and growth allocation across the water table decline treatments, although GA(8) was consistently reduced in plants experiencing water table decline. Because GA(8) is the final gibberellin in the metabolic sequence, it might be useful for assessing historic patterns of GAs and growth rate. This study demonstrated changes in growth allocation in response to water table decline, but provided little evidence that endogenous GAs play a primary role in the regulation of root elongation in response to water table decline. 相似文献
7.
目的]探究抑制剂对大叶黄杨生长的抑制作用以及对其叶片形态和光合作用的影响,为灌木绿篱的化学修剪提供技术指导。[方法]在北京林业大学林场苗圃采用3种生长抑制剂(多效唑(PP_(333))、三碘苯甲酸(TIBA)以及脱落酸(ABA))对密植成绿篱状的1年生大叶黄杨扦插苗进行叶面喷施,对其生长、叶片形态及光合作用等指标进行测定。[结果]3种抑制剂均有矮化植株、抑制新梢生长的作用,矮化效果最佳、抑制作用最强的为PP_(333),且高浓度PP_(333)对于高生长的抑制作用持效性较长。叶宽、叶厚以及叶面积在短期PP_(333)处理下高于对照。PP_(333)能提高大叶黄杨净光合速率,主要通过增加叶厚、气孔导度、叶肉导度及叶绿素含量来实现,并且PP_(333)使蒸腾速率提高的同时降低了水分利用效率。TIBA有显著减小叶长、叶宽的作用,但能使叶厚增加,且随浓度的增加作用效果增强,主要通过减小叶面积,降低叶肉导度、气孔导度、胞间CO_2浓度及叶绿素含量来降低净光合速率。ABA能增加叶面积(10 mg·L~(-1)处理除外)、叶长以及减小叶片厚度,通过抑制气孔导度、叶肉导度、叶绿素含量降低净光合速率。TI-BA及ABA均通过降低蒸腾速率,使水分利用效率提高。[结论]3种抑制剂均有矮化植株、抑制新梢生长的作用,并且影响叶片发育,进而影响叶片光合作用,且800 mg·L~(-1)的多效唑对大叶黄杨具有较好的正向作用。 相似文献
8.
Sha Jiang Ikuo Furukawa Tamaki Honma Miho Mori Teruko Nakamura Fukuju Yamamoto 《Journal of Wood Science》1998,44(5):385-391
The present study deals with the effects of gibberellins (GA3 GA4) and uniconazole-P, an inhibitor of gibberellin biosynthesis, on negative gravitropism and xylem formation in the stems of horizontally positioned, 2-year-oldFraxinus mandshurica Rupr. var.japonica Maxim. seedlings. Each growth regulator (100 g) dissolved in 5 l acetone (50%) was applied to the basal node of the current shoot on May 24, 1995. The same treatment was repeated five times weekly until June 28. Five seedlings were used for each treatment. The seedlings were positioned horizontally 24h after the first application on May 25. Within 5 weeks the horizontal stem of control and GA-treated seedlings exhibited negative gravitropism. In contrast, the application of uniconazole-P inhibited negative gravitropic stem bending. The application of GAs increased the number of gelatinous fibers having thickened cell walls on the upper side of stems. The uniconazole-P application decreased xylem cell formation but did not inhibit the formation of gelatinous fibers. These results indicate that not only the differentiation of gelatinous fibers but also xylem increment is important in the negative gravitropism of horizontally positionedF. mandshurica seedlings. These results also suggest that GAs may be involved in xylem cell :formation rather than the differentiation of gelatinous fibers in this species.Part of this research was reported at the 23rd annual meeting of the Plant Growth Regulation Society of America, Calgary, Canada, July 1996 相似文献
9.
盐胁迫对尖果沙枣离子分布及渗透调节的影响 总被引：2，自引：0，他引：2
为了探讨尖果沙枣的耐盐机理,用NaCl处理尖果沙枣1年生幼苗,处理浓度为50、100、200、300和350 mmol/L,30 d后取样,测定其生理指标。结果表明:随NaCl浓度的增加,叶片膜透性呈增加趋势,叶片水势则有所降低,其中在低于300 mmol/L NaCl浓度下,叶片膜透性和水势的变化幅度不大,在300、350 mmol/L NaCl浓度时与对照呈极显著性差异(P<0.01);随NaCl浓度的增加,脯氨酸含量增加幅度较大,可溶性糖和可溶性蛋白含量均呈较缓的增加趋势,且分别在100 mmol/L和300 mmol/L NaCl浓度下达到最大值,与对照相比,各处理中的有机物质含量均有不同程度增加,其中在350 mmol/L NaCl浓度下的增幅最大;随NaCl浓度的增加,各器官中K 、Ca2 、Mg2 含量变化不大,叶片中K 、Mg2 含量大于茎部和根部,根部Ca2 含量最多,说明植物虽受到一定的伤害,但通过调节渗透调节物质的含量,降低水势,维持其生命代谢,因此,尖果沙枣具有较强的耐盐性。 相似文献
10.
以野生长蕊杜鹃半木质化茎段为试材,采用植物生长激素KIBA,KNAA和Hormodin对插穗进行处理,测定不同生根期内源激素IAA、GA3、ZR、ABA含量,对扦插不同时期插穗茎段进行电镜扫描。结果表明:先快蘸液态KNAA(3 000mg·L-1),再迅速蘸取粉状Hormodin#1处理对插条生根具有显著影响,插穗生根率达到了81.25%;较高浓度的内源IAA含量有利于根原基分化形成,GA3含量上升与插穗愈伤组织的诱导、不定根的形成呈正相关性,低水平的ABA更有利于长蕊杜鹃扦插生根;插穗在根原基分化和愈伤组织形成时期消耗內源ZR,在不定根长出后自身合成ZR;通过对淀粉粒研究表明扦插期补充糖类物质是必要的,簇状晶体产生表明钙调控等生理机制形成。 相似文献
11.
In order to understand the content changes of endogenous hormones in lateral buds of Populus yunnanensis during different seasons, the lateral buds collected from stems of three-year old cuttings of P. yunnanensis were used as materials, contents of ABA, IAA and ZR in the lateral buds in the autumn, winter and spring were detected by enzyme-linked immunosorbent assays (ELISA). The results indicated that the contents of ABA and IAA were first decreased and then increased while the contents of ZR increased gradually, and the values of the three kinds of endogenous hormones reached maximum in spring. The content of each endogenous hormone among different seasons was different significantly. However, the ratio changes of ZR/ABA, ZR/IAA and ZR/(ABA+IAA) were first increased and then decreased, and all reached the maximum in winter, which were significantly higher than that in autumn and srping. 相似文献
12.
本文研究了广西人工林马尾松木材性质的变异及幼龄材与成熟材的差异。结果表明 ,幼龄材与成熟材的分界年龄在 14 16a ,解剖性质在径向上的变异规律为 :射线比量、树脂道比量、胞壁率、胞腔直径、胞壁厚、管胞长度、管胞宽度和晚材壁腔比是自髓心向外呈递增趋势 ,管胞比量和晚材率为递减趋势 ,早材壁腔比和早材腔径比则近似于一条直线。方差分析结果表明 :树脂道比量、胞壁率、胞壁厚、管胞长度和管胞宽度 ,幼龄材与成熟材差异达显著或极显著水平。 5项木材物理力学性质均为成熟材高于幼龄材 ,且均达差异显著水平。木材性质间的相关分析表明 :木材基本密度与管胞长度、管胞宽度、射线比量、树脂道比量、胞壁率呈显著的正相关关系 ,木材气干密度与抗弯强度、抗弯弹性模量、顺纹抗压强度也呈显著的正相关关系 相似文献
13.
We investigated the involvement of gibberellins (GAs) and indole-3-acetic acid (IAA) in the control of longitudinal and cambial growth in current-year shoots of Pinus sylvestris L. Elongating terminal shoots, located at the apex of previous-year (1-year-old) branches in the uppermost whorl on the main stem, were variously decapitated (apical 5 to 10 mm removed), defoliated (all developing needle fascicles removed) and treated with endogenous GA(4/7) or IAA, or both. Shoot length and the radial widths of xylem and phloem were measured, and the concentrations of GA(1), GA(3), GA(4), GA(9) and IAA in the stem were determined by combined gas chromatography-mass spectrometry with deuterated GAs and [(13)C(6)]-IAA as internal standards. Decapitation decreased the production of xylem and phloem and the IAA concentration, but did not alter either longitudinal growth or the concentrations of GAs. Defoliation markedly inhibited shoot elongation, as well as cambial growth, and reduced the concentrations of GA(1), GA(3), GA(4), GA(9) and IAA. Application of GA(4/7) to defoliated shoots promoted longitudinal growth and phloem production, without affecting xylem production or IAA concentration. Application of GA(4/7) and IAA together to decapitated + defoliated shoots increased shoot elongation, xylem and phloem production and IAA concentration, whereas applying either substance alone had a smaller effect or none at all. We conclude that, for elongating current-year shoots of Pinus sylvestris, (1) both the shoot apex and the developing needle fascicles are major sources of the IAA present in the stem, whereas stem GAs originate primarily in the needle fascicles, (2) GAs and IAA are required for both shoot elongation and cambial growth, and (3) GAs act directly in the control of shoot growth, rather than indirectly through affecting the IAA concentration. 相似文献
14.
Intact seeds from freshly harvested fruits of Myrica rubra (Sieb et Zucc.) were dormant and required 8 weeks of warm stratification followed by 12 weeks of cold stratification for germination. Exogenous application of gibberellic acid (GA(3)) to intact fresh seeds was effective in breaking dormancy, with > 70% of seeds germinating when treated with 5.2 mM GA(3) and incubated at a day/night temperature of 30/20 degrees C for 20 weeks. Removing the hard endocarp or endocarp plus seed coat of fresh seeds promoted germination, and addition of GA(3) to the embryo accelerated germination. The gibberellins GA(1) and GA(4) were more effective than GA(3) in promoting germination of seeds with the endocarp removed. Endogenous contents of GA(1), GA(3), GA(4), GA(7) and GA(20) were quantified by gas chromatography-mass spectrometry-selected ion monitoring in the endocarps, seed coats and embryos of fresh seeds treated with 5.2 mM GA(3). The content of GA(3) decreased in the endocarp during incubation, whereas GA(1) contents increased in the endocarp and seed coat. A high GA(1) content was detected in the endocarps and embryos of newly germinated seeds. We speculate that GA(3) was converted to GA(1) during incubation and that GA(1) is involved in seed germination. Endogenous abscisic acid (ABA) contents were measured in fresh seeds and in warm and cold stratified seeds. The ABA content in fresh seeds was distributed in the order endocarp > seed coat > embryo, with the content in the endocarp being about 132-fold higher than in the seed coat and embryo. Total ABA content of seeds subjected to warm or cold stratification, or both, was 8.7- to 14.0-fold lower than that of fresh seeds. Low contents of endogenous GA(1), GA(3), GA(7) and GA(20), but elevated contents of GA(4), were found in the seed coats and endocarps of warm plus cold stratified seeds and in the seed coats and embryos of newly germinated seeds. These observations, coupled with the finding that GA stimulated germination of dormant Myrica seeds, provide evidence that endogenous ABA inhibited release of dormancy and that endogenous gibberellins, especially GA(4) or GA(1), or both, are involved in germination. 相似文献
15.
16.
Xiangxiang Fu Hongna Liu Jie Xu Jiajia Tang Xulan Shang 《Scandinavian Journal of Forest Research》2014,29(6):542-551
The combination of gibberellin acid (GA) soaking with moist chilling plays a pivotal role in seed dormancy breaking. However, knowledge involving physiological and biochemical mechanisms for such a response is limited. This work focused on the effects of GA3 and moist chilling on overcoming seed dormancy as well as the response of endogenous hormones and nutrient reserves to dormancy release in Chinese dogwood (Cornus kousa var. chinensis). Seeds subjected to GA3 soaking and moist chilling exhibited a high germination percentage (average 97%), and the combination of 500 mg·L?1 GA3 soaking for 3 days followed with moist chilling for 50 days was more effective than other treatments. For GA3 treatments, lipids were initially hydrolyzed during the first stage of moist chilling (0–20 days), resulting in the increase of starch and soluble sugars, while proteins were kept relatively stable. During the second stage (20–50 days), contents of lipids, starch, soluble sugars, and proteins decreased by 46%, 82%, 61% and 59%, respectively. Abscisic acid (ABA) contents and ratios of ABA to GA3 in GA3 treatments decreased by nearly 70% and over 90%, respectively, when dormancy was terminated. However, endogenous GA3 contents in all treatments significantly increased with prolonged moist chilling. The present results provide insight into the metabolic mechanism involving hormonal regulation and mobilization of reserves during the release of seed dormancy in Chinese dogwood. 相似文献
17.
《Scandinavian Journal of Forest Research》2012,27(1-4):83-95
Nine seed lots each of Norway spruce (Picea abies L.), and Scots pine (Pinus sylvestris L.) were treated with combinations of the growth regulators: Indole‐3‐acetic acid, indole‐3‐butyric acid, etephone, kinetin, fusicoccin, and the gibberellins A1 A3 A4/7, and A9. GA9 GA4/7 and fusicoccin significantly promoted the germination of five Norway spruce, and three Scots pine, seed batches. Independent of species, the germinability of seeds treated with auxins, etephone, kinetin, GA1 and GA3 were unaffected or reduced. The rate of germination was stimulated for both species when treated with GA9 or GA4/7. The germination percentage for Scots pine seeds was enhanced by GA9 but not by GA4/7. Neither of the two GAs enhanced the germination percentage of Norway spruce seeds. 相似文献
18.
核桃幼树内源激素与生长势的关系 总被引：5，自引：0，他引：5
激素在果树生长发育过程中起着重要调控作用,基因通过激素控制植株高矮(曾骧,1992).对苹果(Maluspumila)、李(Prunus salicina)、桃(Prunus persica)等果树内源激素与生长势的关系已有一些报道(王丽琴等,2002;周怀军等,2002;孙艳香等,2001),但在核桃上有关研究尚少.本试验以核桃(Juglans regia)不同品种的嫁接植株及实生苗为试材,探讨内源激素与树体生长势的关系,为选择核桃矮化砧木提供理论依据. 相似文献
19.
大小年毛竹林中内源激素节律变化特征的研究 总被引：5，自引：0，他引：5
运用酶联免疫测定法（ＥＬＩＳＡ）,对大小年毛竹林一个大小年生长发育周期中,不同生长发育时期竹叶、竹秆、竹鞭、鞭梢中内源激素（ＩＡＡ、ＧＡ_３、ＡＢＡ）含量进行了测定和分析,结果表明：（１）大小年毛竹林鞭竹系统中任一器官中ＩＡＡ、ＧＡ_３含量的变化趋势一致,ＡＢＡ含量的变化趋势与ＩＡＡ、ＧＡ_３的相背。（２）立竹的内源激素节律变化,具相对独立性,竹叶、竹秆中内源激素含量的变化具同步性,ＩＡＡ、ＧＡ_３、ＡＢＡ含量各出现两个峰值和两个低值;ＩＡＡ、ＧＡ_３大小年年均含量大年高小年低,ＡＢＡ则相反。（３）鞭梢中ＩＡＡ、ＧＡ_３、ＡＢＡ含量变化也具相对独立性,出现二个峰值和二个低值;ＩＡＡ、ＧＡ_３、ＡＢＡ大小年年均含量基本相当。（４）竹鞭中内源激素的节律变化具整体性,分别是四个峰值和四个低值;ＩＡＡ、ＧＡ_３、ＡＢＡ大小年年均含量基本相当。毛竹大小年的形成是由毛竹林鞭竹系统的整体性结构和立竹个体的生长节律及植物内源激素的调控作用共同决定的。 相似文献
20.
Fan Zhang Xue Qin Wan Hai Qing Zhang Guang Li Liu Ming Yan Jiang Yuan Zhi Pan Qi Bing Chen 《Journal of Forest Research》2012,17(1):72-78
An unusually severe snow disaster in southern China in 2008 exposed variation among different species of bamboo: some species
were highly tolerant to low-temperature damage, but some suffered extensive damage or death. Therefore, this study was conducted
to determine the effects of cold stress on endogenous hormones and the expression of homologs of CBF
1
in four bamboo species differing in their tolerance to cold. All four bamboo species were subjected to cold stress with a
temperature gradient (10, 5, 0, −5, and −10°C), and each low temperature treatment was maintained for 48 h. Results showed
that ABA, GA1, and GA4 were significantly affected by cold stress: ABA increased with cold stress in all species, while GA1 and GA4 reduced with cold stress. ABA levels and the ratio of ABA/(GA1 + GA4) in leaves of Neosinocalamus affinis and Bambusa rigida were both higher than those of hybrid bamboo (Bambusa pervariabilis × Dendrocalamopsis grandis) and Dendrocalamus latiflorus for all treatments, while GA1 and GA4 were conversely lower as compared to the hybrid variety and D. latiflorus. Moreover, CBF
1
homolog was up-regulated during cold stress, with higher expression levels in the leaves of cold-tolerant species than cold-sensitive
ones. Results revealed that high levels of ABA and CBF
1
, as well as the ABA/GAs balance, are important in increased plant resistance to cold stress. 相似文献