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为了解高致病性猪蓝耳病在涪陵区生猪养殖场的抗体免疫保护率情况,以及为该区防治动物疫病提供科学依据,2010年—2012年对涪陵区25个集约化生猪养殖场进行了定点血清样品采集,分别采集了能繁母猪和仔猪的血清样品各500份,共1000份,然后利用免疫血清学抗体检测试验对蓝耳病抗体进行了血清学抗体检测及分析。结果表明:2012年400份血清样品中蓝耳病抗体阳性的血清有336份,阳性率为84%,2011年蓝耳病抗体阳性率为70%,2010年蓝耳病阳性率为55%,2012年与前两年血清抗体监测水平对比均有提高。 相似文献
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目的分析猪群中口蹄疫、猪瘟、高致病型蓝耳病这3种主要疫病免疫抗体水平监测与效果。方法采用整群抽样法选取20个规模生猪养殖场以及40个散养户作为研究对象,采集生猪耳静脉血或者是前腔静脉血后对猪群三种主要疫病(生猪口蹄疫、猪瘟、高致病性猪蓝耳病)抗体水平进行检测并分析免疫效果。结果 20个规模生猪养殖场采集200头生猪血液样本,40个散养户采集400头生猪血液样本,对免疫抗体水平进行测定后对比发现规模生猪养殖场生猪口蹄疫、猪瘟、高致病性猪蓝耳病三种抗体阳性率均高于散养户,但差异无统计学意义(P0.05),无论是规模生猪养殖场还是养殖户抗体阳性率均高于国家及地方标准规定(70%)。结论规模生猪养殖场生猪的口蹄疫、猪瘟、高致病性蓝耳抗体水平监测结果普遍好于散养户,二者均符合国家及地方标准规定但仍有进一步提升空间,故强化防疫监管以及免疫疫病抗体监测仍十分重要。 相似文献
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<正>生猪规模养殖场的发展除与生猪养殖技术、猪场管理、市场行情和防疫免疫以外,猪场的规范消毒技术的运用是其重要的因素。目前在农村,特别是一些经济不发达的农村,生猪规模养殖场的消毒技术很不规范,猪场的许多疾 相似文献
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目的:为了解西宁市所销售消毒纯牛奶中抗生素残留的情况。方法:利用氯化三苯四氮唑(TTC)指示剂还原变色原理对来自不同品牌、不同产地的40份消毒纯牛奶进行检测。结果:检出抗生素残留阳性牛奶2份,可疑牛奶3份,阳性率5.0%,可疑率7.5%,其中青海省内生产的消毒纯牛奶抗生素残留阳性牛奶1份,可疑牛奶2份,青海省外生产的消毒纯牛奶抗生素残留阳性1份,可疑牛奶1份。结论:西宁市售消毒牛奶有一定程度的抗生素残留。 相似文献
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应用常规细菌分离法对西宁市某几个农贸市场采集的70份仔虾样品进行了副溶血性弧菌的检测,经细菌形态观察、嗜盐性试验、细胞色素氧化酶试验和生化特性测定,鉴定为副溶血性弧菌。结果检出阳性菌株4株,阳性率为5.7%(4/70),同时通过致病性试验证明4株分离菌对小白鼠有致死效应。 相似文献
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为进一步了解青海湖裸鲤(Gymnocypris przewalskii)养殖池塘中养殖密度与浮游动物群落结构间的关系,通过对不同养殖密度的青海湖裸鲤池塘进行连续的采样调查,测定浮游动物的群落结构、水质指标以及青海湖裸鲤的生长情况。讨论并分析青海湖裸鲤养殖密度对池塘浮游动物群落结构的影响。结果显示:试验期间共测定出浮游动物38种,以轮虫为主,其数量占整个浮游动物种类的61. 5%;浮游动物密度、生物量、多样性指数在中、高养殖密度条件下均随着养殖时间的增加呈现下降趋势。研究表明:高密度养殖对浮游动物群落结构影响较大,中、高密度养殖鱼苗对浮游动物的高摄食压力使浮游动物生长受到限制,浮游动物种类减少并趋于小型化,不利于养殖水环境的稳定。 相似文献
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In a comprehensive epidemiological study at the I. Medizinische Tierklinik at the Ludwig-Maximilians-University in Munich within the last two years, the spread and frequency of FIV infection as well as the clinical symptoms of FIV positive cats were evaluated. 5129 serum samples from clinically healthy and ill cats were tested by means of an ELISA test for antibodies to FIV. The infection is widespread in Bavaria. 116 cats, that is 2.3%, were tested FIV positive. 77% of the FIV positive cats were male. The average age was 6.5 years. 相似文献
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Abstract. Sexually mature spawning and prespawning adult and 'jack' coho salmon, Oncorhynchus kisutch , were collected during their potamodromous migration from Lakes Ontario, Erie and Michigan in late autumn. In addition, sexually immature spring salmon were collected from Lakes Ontario and Erie. Serum total thyroxine (TT4), serum free thyroxine (FT4), serum total triiodothyronine (TT3) and serum free triiodothyronine (FT3) levels showed marked seasonal variability in salmon from Lakes Ontario and Erie with peak hormone levels evident in the spring fish and lowest levels in the sexually mature adults and 'jacks'. Seasonal changes in serum triiodothyronine uptake (T3U) values were inverse of those of serum TT4, FT4, TT3 and FT3 levels. When data of the whole collection were combined significant positive correlation coefficients were evident as follows: serum TT3 v. serum TT4, FT4 and TT3; serum TT4 v. serum FT4 and FT3; serum FT4 v. serum FT3, and significant negative correlations as follows: serum T3U v. serum TT3, TT4, FT4 and FT3. When individual groups were examined significant correlations were either absent, or in some instances the reverse of those of whole data. The data provide further evidence of interlake differences in dysfunction of thyroid hormone metabolism in Great Lakes coho salmon; the possible involvement of environmental agents is discussed. 相似文献
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Abstract. The effectiveness of three different growth media KDM-2, S-KDM and S-KDM-C for primary isolation of Renibacterium salmoninarum was examined over a 14-week incubation period. Kidney samples were taken from Atlantic salmon, Salmo salar L., broodfish returning to a sea ranch after 2 years at sea. Homogenized samples were inoculated onto two selective media, S-KDM supplemented with lamb serum and S-KDM-C supplemented with activated charcoal. The third medium was the non-selective serum supplemented KDM-2. Renibacterium salmoninarum was isolated from 112 samples on one or more of the media used. Of all positive samples, 91% were positive on S-KDM, 60% on S-KDM-C and 35% on KDM-2. These results demonstrate that selectivity significantly enhances the isolation capacity of the medium and that supplementing with serum is significantly more effective than supplementing with charcoal. 相似文献
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The milk pools of two cattle herds in Lower Saxony were found to give positive results in an enzyme-linked immunosorbent assay (ELISA). The sera from these cattle were tested in competition enzyme immunoassays (cEIA) using two monoclonal antibodies. The results were compared to those obtained in the serum agglutination assay (SLA) and in an ELISA. The cEIAs detected more positive samples than the other tests. All sera that were positive in the SLA and in the ELISA were also positive in the cEIAs. Due to the higher sensitivity, these tests may be an alternative for the SLA. 相似文献
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Robert P. Wilson Delbert M. Gatlin William E. Poe 《Aquaculture (Amsterdam, Netherlands)》1985,49(2):101-110
Five purified diets were utilized to investigate the effect of varying protein and energy intake on postprandial changes in systemic serum amino acid levels of channel catfish (Ictalurus punctatus). The diets contained 25, 30 or 35% crude protein at one of three different energy levels. Systemic serum free amino acids and glucose were determined at 0, 2, 4, 8, 12 and 24 h after feeding each diet. In general, for each diet tested, most of the systemic serum free amino acid levels increased within 2 h after feeding, remained elevated for up to 12 h, and returned to fasting levels within 24 h after feeding. Varying the protein to energy ratios in the diets did not profoundly affect postprandial systemic serum free amino acid patterns. There was a significant positive correlation between the levels of systemic serum free essential amino acids and dietary levels. No such correlation was observed for non-essential amino acids. Serine and alanine were the most abundant free amino acids in catfish serum. Glutamic acid, glycine and proline remained relatively low throughout all treatments. A reciprocal relationship was observed between levels of glucose and total free amino acids in the serum. 相似文献
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在长江口中华绒螯蟹(Eriocheir sinensis)亲蟹生殖洄游期间,对比研究了不同性腺发育时期的长江口中华绒螯蟹雌性亲蟹自然群体和放流群体9项血淋巴生化指标的差异。结果表明,洄游亲蟹在性腺发育的不同时期,放流群体与自然群体血淋巴总蛋白、血蓝蛋白、甘油三酯、肌酐、Ca2+及Mg2+含量水平上均无显著差异(P>0.05)。放流群体亲蟹在性腺发育到Ⅳ、Ⅴ期时的血淋巴白蛋白含量和性腺发育到Ⅳ期时尿素含量均显著高于自然群体(P<0.05),总胆固醇含量在性腺发育到Ⅵ期时显著高于自然群体(P<0.05)。在性腺发育过程中放流群体的大部分血淋巴生化指标与自然群体都无显著差异,仅在性腺发育到Ⅳ期后少数指标存在一定差异,表明放流群体通过自身调节能够很好地适应长江口环境并完成性腺发育过程。 相似文献
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利用微创手术检查的方法对人工养殖西伯利亚鲟的卵巢发育期进行鉴别,根据卵巢发育期将西伯利亚鲟分为Ⅱ期、Ⅲ期、Ⅳ期、Ⅴ期和Ⅵ期共5个实验组。对5个实验组血清脂蛋白脂酶(LPL)活性及血脂含量进行测定,并对其之间的关系进行分析。结果表明:随着卵巢发育,血清LPL活性、极低密度脂蛋白胆固醇(VLDL-C)、甘油三酯(TG)呈"先上升,后下降"的趋势,其中LPL在Ⅴ期达到最高值,VLDL-C、TG均在Ⅳ期达到最高值;总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)随卵巢发育表现为上升趋势。血清TG与LPL呈正相关关系。 相似文献