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1.
低温锻炼提高了毛白杨幼苗叶片和枝条中钙调素 (CaM)和可溶性总蛋白含量 ,同时也提高了幼苗的抗冻性 .CaCl2 处理对上述低温锻炼的作用有加强的效果 ,体现在明显提高了CaM和可溶性总蛋白含量以及幼苗抗冻性 ;钙离子螯合剂EGTA、钙离子通道阻断剂LaCl3 或钙调素拮抗剂CPZ处理则减弱了低温锻炼的效应 ,表现出CaM和可溶性总蛋白含量以及幼苗抗冻性明显降低 .进一步研究发现 ,CaCl2 处理的低温锻炼不仅能减轻低温胁迫引起的CaM和可溶性总蛋白含量的下降程度 ,而且有利于幼苗在恢复过程中CaM和可溶性总蛋白水平的迅速回升 .这表明Ca2 + CaM可能参与了CaM和可溶性总蛋白的合成及抗冻性的诱导 相似文献
2.
以零下低温锻炼和结合效应剂（CaCl2、钙离子螯合剂EGTA、钙离子通道阻断剂LaCl3或钙调素拮抗剂CPE）处理的低温锻炼下的中东扬扦插苗为试材，对其体内丙二醛（MDA）及钙调素CaM含量，SOD、POD及线粒体Ca^2-＋ATPase活性分别进行测定。结果表明，低温锻炼不仅在一定程度下提高了幼苗CaM含量，提高了SOD、POD和Ca^2＋-ATPase活性，降低了MDA含量；而且减少了低温胁迫所引起的SOD、POD，Ca^2＋＋ATPase和CaM含量的下降程度以及MDA的增加幅度。在低温锻炼的同时。用CaCl2处理能加强低温锻炼的效果，但这种效应可被EGTA、LaCl3或CPZ处理抑制。经低温锻炼后，幼苗中的CaM含量的增加有助于SOD、POD和线粒体Ca^2＋-ATPase活性的提高，进而对幼苗抗冻性的提高有明显的促进作用。 相似文献
3.
在低温锻炼和结合蔗糖处理的低温锻炼下 ,对毛白杨 (Populustomentosa)幼苗枝条和叶片的可溶性糖含量 ,以及幼苗存活率和抗冻性分别进行了测定 .结果表明 ,低温锻炼明显提高了幼苗枝条和叶片的可溶性糖含量 ,同时也提高了幼苗的存活率和抗冻性 .2 0 %蔗糖处理对上述低温锻炼的作用有加强的效果 .进一步分析发现 ,无论是单纯的低温锻炼还是结合蔗糖预处理的低温锻炼 ,幼苗枝条中可溶性糖含量的提高程度均较叶片明显 ,而且它们的含量增加与幼苗抗冻性的提高密切相关 .这表明低温锻炼所引起的可溶性糖积累可能参与了幼苗抗冻性的诱导 相似文献
4.
首次研究了低温锻炼对甜杨 (Populussuaveotlens)幼苗抗冻性的效应 ,并对低温锻炼中的G6PDHase ,ATPase及蛋白质的动态变化过程进行了测定 .另外 ,在低温锻炼前用蛋白质合成抑制剂环己亚胺对甜杨幼苗进行预处理 .试验结果表明 ,适当的低温锻炼可明显提高甜杨幼苗的抗冻性 ,但整个低温锻炼过程必须分两个阶段进行 ,第一阶段在 - 10℃的温度下锻炼 6天 ,虽对甜杨幼苗抗冻性的提高效应不明显 ,但它是不可缺少的 ,它可为第二阶段的 - 2 0℃锻炼的进行及抗冻性的完全发育提供基础 .伴随着幼苗枝条中蛋白质含量及G6PDHase和ATPase活性提高的同时 ,也明显提高了幼苗的抗冻性 ;脱锻炼 2天后 ,幼苗抗冻性下降到未锻炼水平 ,而枝条中蛋白质含量及G6PDHase和AT Pase活性虽有下降但略高于未锻炼 .蛋白质合成抑制剂环己亚胺预处理则明显降低了幼苗抗冻性和蛋白质含量 .进一步分析发现 ,低温锻炼中的G6PDHase和ATPase活性及蛋白质含量的变化与幼苗抗冻性的提高密切相关 相似文献
5.
在低温锻炼中毛白杨幼苗可溶性总蛋白、RNA、DNA、RNase及抗冻性的变化 总被引：4，自引：0，他引：4
低温锻炼不仅提高了毛白杨幼苗存活率和抗冻性以及RNA和可溶性总蛋白的含量 ,降低了RNase活性 ,而且能减轻低温胁迫引起的RNA和可溶性总蛋白含量的下降程度和RNase活性的提高 ,有利于幼苗在恢复过程中RNA和可溶性总蛋白水平的迅速回升以及RNase活性的降低 .进一步研究发现 ,DNA含量无论在低温锻炼中还是在低温胁迫下或是在随后的恢复生长期均保持相对稳定 ;低温锻炼所引起的RNA含量的增加 ,与RNase活性的降低呈明显的负相关 ,与可溶性总蛋白含量的增加以及幼苗抗冻性的提高成正相关 .这表明低温锻炼可能抑制RNase活性 ,有效地促进RNA含量的增加 ,而RNA可能参与了可溶性总蛋白的合成及抗冻性的诱导 相似文献
6.
PP333与CaCl2对绿玉树扦插苗抗寒性的影响 总被引：8，自引：0，他引：8
对绿玉树扦插苗喷洒不同浓度、不同配比的PP333(多效唑)和CaCl2，以提高绿玉树的抗寒能力。试验结果表明：多效唑能显著降低新梢长度的生长，增加分枝数，抑制低温处理时膜透性的增加，提高绿玉树对零上低温的抵抗能力。0．2％PP333能提高叶绿素含量，但低浓度的PP333(0．1％-0．15％)却降低了叶绿素的含量。CaCl2对绿玉树扦插苗的形态影响不明显，0．015％-0．1％的CaCl2能抑制低温下细胞膜透性的增加，提高叶绿素含量。 相似文献
7.
8.
低温锻炼对冷胁迫下油茶幼苗光合速率与抗氧化酶活性的影响 总被引：3，自引：0，他引：3
以油茶品种"杂优闽1"为试验材料,以未经低温锻炼的油茶幼苗为对照,研究了油茶幼苗在(10±0.5)℃低温锻炼、(2±0.5)℃冷胁迫和常温恢复期间的光合速率和抗氧化酶活性的变化。结果表明:低温锻炼3 d可明显提高油茶幼苗的抗寒性;冷胁迫3 d,经低温锻炼后的油茶幼苗叶片净光合速率和相对含水量明显高于对照;且其在常温下的恢复也较对照迅速。在低温锻炼和冷胁迫期间,油茶幼苗叶片脯氨酸和可溶性糖含量明显增加,且丙二醛(MDA)含量明显低于对照;经低温锻炼的油茶幼苗叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性则均明显高于未经低温锻炼的油茶幼苗,说明渗透调节物质含量增加和保护酶活性增强在低温锻炼诱导油茶幼苗的抗寒性上发挥着重要的作用。 相似文献
9.
以"津研7号"和"津春4号"黄瓜幼苗为材料,在其生长期外施不同浓度的H2O2,研究了H2O2对黄瓜幼苗抗低温胁迫的影响.结果表明:黄瓜幼苗经低温胁迫后,其电导率和丙二醛含量急剧上升,而过氧化物酶活性和可溶性蛋白含量明显降低;与对照相比,外施H2O2能提高其过氧化物酶活性,降低膜渗漏率,增加可溶性蛋白含量,减缓膜脂过氧化作用,从而提高黄瓜幼苗的抗低温胁迫能力,其中以10 mmol.L-1的H2O2处理效果最为明显. 相似文献
10.
喷施壳聚糖对核桃抗冻性的影响 总被引：1，自引：0，他引：1
通过对核桃枝条短期低温胁迫研究,探讨低温胁迫条件下核桃枝条内的生化代谢过程与树体抗冻能力之间的关系。通过对离体香玲核桃枝条以—2、0、4℃低温处理,并在处理后不同时间测定相关酶活性与抗冻蛋白含量,分析低温胁迫后树体抗冻相关酶活性和蛋白含量。研究结果发现,在—24℃的低温胁迫均能够显著提高核桃枝条内SOD、CAT、POD酶的活性,同时脯氨酸的含量在04℃的低温胁迫均能够显著提高核桃枝条内SOD、CAT、POD酶的活性,同时脯氨酸的含量在04℃的低温胁迫下也有显著增加。说明SOD、CAT、POD和脯氨酸参与了核桃树体的抗冻反应。 相似文献
11.
LinShanzhi GuoHuan LiuWenfeng LinYuanzhen ZhangQian HuDongmei ZhuBaoqing ZhangZhiyi 《中国林学(英文版)》2004,6(4):1-7
Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) was purified from the leaves of 8-week-old Populus suaveolens cuttings. The enzyme activity in the absence and presence of reduced dithiothreitol (DTTred) was determined. The results show that the G6PDH activity is not inactivated by pre-incubation with DTTred, indicating that the purified enzyme probably presented in cytosol of P suaveolens. The catalytic characteristics and kinetic parameters of cytosolic G6PDH purified from P.suaveolens cuttings were also studied. The results show that G6PDH is characterized by Km value of 360/amol.L^-1 for G6P and 16μmol.L^-1 for NADP, a pH range of 7.3-8.9, and the maximum activity around pH 8.2. The enzyme activity is inhibited by various metabolites such as NADPH, NADH, GTP, UTP, ATP, AMP, ADP, CoA, acetyl CoA, fructose-6-phosphate (F6P), erythrose-4-phosphate (E4P), ribose-5-phosphate (R5P) and 3-phosphoglycerate (3-PG) (all at 1 mmol‘L i except for NADPH and NADH)to different extents. NADPH is the most effective inhibitor of enzyme activity, with an inhibition of 72.0%. The addition of metal ions such as MgC12, CaC12 and KC1 (all 1.0 mmol.L^-1) to the standard reaction mixture has no remarkable influence on the cytosolic G6PDH activity. However, CdCl2 (1.0 mmol.L-1) causes high inhibitory effect on the enzyme activity. To explore the role of G6PDH on the enhancement of freezing resistance induced by freezing acclimation, the changes in the cytosolic G6PDH activity and freezing resistance (expressed as LTs0) of P. suaveolens cuttings during freezing acclimation at -20℃ were investigated. The results reveal that freezing acclimation decreases LTs0 of cuttings, and increases the activity of cytosolic G6PDH compared with control ones,while 2 d of de-acclimation at 25 ~C result in a decrease in cytosolic G6PDH activity, and caused an increase in LT50. Furthermore,the change in cytosolic G6PDH activity is found to be closely correlated to the degree of freezing resistance of cuttings during freezing acclimation. It is suggested that cytosolic G6PDH may be involved in the induction of freezing resistance of cuttings. 相似文献
12.
毛白杨幼苗抗氧化系统对盐胁迫的响应 总被引：4，自引：0，他引：4
在 3种不同浓度的NaCl (85mmol·L-1,170mmol·L-1,2 6 0mmol·L-1)胁迫下 ,对毛白杨幼苗的超氧化物歧化酶 (SOD)和过氧化物酶 (POD)活性以及丙二醛 (MDA)含量进行测定 .结果发现 ,在NaCl胁迫 3小时后 ,幼苗SOD和POD活性显著降低 ,随着胁迫时间的延长 ,这两种酶活性降低较缓慢 ,但SOD活性下降程度均较POD小 .在NaCl胁迫中 ,MDA含量明显增加 ,并于胁迫 15小时后达到最大值 ,随后含量增加较小 .进一步分析发现 ,不同浓度NaCl处理对SOD和POD活性以及MDA含量的作用效应是不同的 ,其中 2 6 0mmol·L-1NaCl处理明显促进幼苗SOD和POD活性的下降与MDA含量的增加 ;而且MDA含量与SOD和POD活性的下降有关 .结果表明 ,NaCl胁迫所引起的SOD和POD活性下降可能加强膜脂过氧化作用 ,最终导致幼苗MDA含量的增加与伤害的发生 相似文献
13.
Comparison of G6PDH Activity and LT50 Between P.tomentosa and P.suaveolens During Freezing Acclimation 总被引：3，自引：0，他引：3
LinShanzhi ZhangZhiyi LiuYuanzhen 《中国林学(英文版)》2003,5(1):20-24
The differences of glucose-6P dehydrogenase(G6PDH) activity and freezing resistance induced by freezing acclima-tion between cuttings of freezing-sensitive P.tomentosa and freezing-resistant P.suaveolens were compared for exploring the role of G6PDH on the enhancement of freezing resistance induced by freezing acclimation.After 5d of freezing acclimation at -3℃ ,the LT50 of P.tomentosa has deereased from -6.2℃ incontrol cuttings to -14.3℃ in freezing acclimated ones,and the increase of LT50 of P.tomentosa has decreased from -6.2℃ in control cutting to -14.3℃ in freezing acclimated ones,and the increase of G6PDH activity was observed in freezing acclimated cuttings as compared with control ones.Whereas,when P.suaveolens was freezing acclimated at -20℃ for 5d,the LT50 has decreased from -27.1℃ in control cuttings to -43.5℃ in freezing acclimated ones,and the activity of G6PDH increased considerably.In addition,the increase of LT50 and the decrease of G6PDH activity resulting from 2d of deacclimation at 25℃ were found in two kinds of freezing acclimated cuttings.It is concluded that the increase in the activity of G6PDH may associate with the inherited freezing resistance of species and the enhancemen of freezing resistance of cuttings,and may play an important role in the antifreeze process under freezing temperature,which would provide the basis for the study on the molecular mechanism of freezing resistance in P.suaveolens and the cloning of gene associated with freezing resistance. 相似文献
14.
The role of antioxidant system in freezing acclimation-induced freezing resistance of Populus suaveolens cuttings 总被引：1，自引：0，他引：1
We investigated the changes in the contents of H2O2, malonaldehyde (MDA) and endogenous antioxidants, the activities of protective enzymes and some critical enzymes involved in the ascorbate-glutathione (ASA-GSH) cycle as well as freezing resistance (expressed as LT50) and correlations mentioned above, in detail using Populus suaveolens cuttings. The purpose was to explore the physiological mechanism of the enhancement of freezing resistance induced by freezing acclimation at –20°C, and to elucidate the physiological mechanisms by which trees adapt to freezing. The results showed that freezing acclimation enhanced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), monodehydroascorbate reductase (MDAR), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR). And it increased the contents of reduced ascorbate (ASA), reduced glutathione (GSH), dehydroascorbate (DHA) and oxidized glutathione (GSSG). However, H2O2 and MDA contents and LT50 of cuttings were decreased. LT50 in cuttings was found to be closely correlated to the levels of SOD, POD, CAT, APX, DHAR, MDAR, GR, H2O2, MDA, ASA, GSH, DHA and GSSG during freezing acclimation. This suggested that the enhancement of freezing resistance of cuttings induced by freezing acclimation may relate to the distinct increase for the levels of SOD, POD, CAT, APX, DHAR, MDAR, GR, ASA, GSH, DHA, and GSSG. In addition, the observed levels of APX, DHAR, MDAR, GR, ASA, DHA, GSH and GSSG were higher than those of SOD, POD and CAT during freezing acclimation. It indicated that a higher capacity of the ASA-GSH cycle is required for H2O2 detoxification, and growth and development of cuttings. Based on the obtained results, it can be concluded that the ASA-GSH cycle plays an important role in enhancement of freezing resistance of P. suaveolens cuttings during freezing acclimation. 相似文献
15.
Lin Shanzhi Guo Huan Liu Wenfeng Lin Yuanzhen Zhang Qian Hu Dongmei Zhu Baoqing Zhang Zhiyi 《中国林学(英文版)》2004,6(4):1-7
Glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) was purified from the leaves of 8-week-old Populus suaveolens cuttings. The enzyme activity in the absence and presence of reduced dithiothreitol (DTTred) was determined. The results show that the G6PDH activity is not inactivated by pre-incubation with DTTred, indicating that the purified enzyme probably presented in cytosol of P. suaveolens. The catalytic characteristics and kinetic parameters of cytosolic G6PDH purified from P. suaveolens cuttings were also studied. The results show that G6PDH is characterized by K
m value of 360 μmol·L−1 for G6P and 16 μmol·L−1 for NADP, a pH range of 7.3-8.9, and the maximum activity around pH 8.2. The enzyme activity is inhibited by various metabolites
such as NADPH, NADH, GTP, UTP, ATP, AMP, ADP, CoA, acetyl CoA, fructose-6-phosphate (F6P), erythrose-4-phosphate (E4P), ribose-5-phosphate
(R5P) and 3-phosphoglycerate (3-PG) (all at 1 mmol·L−1 except for NADPH and NADH) to different extents. NADPH is the most effective inhibitor of enzyme activity, with an inhibition
of 72.0%. The addition of metal ions such as MgCl2, CaCl2 and KCl (all 1.0 mmol·L−1) to the standard reaction mixture has no remarkable influence on the cytosolic G6PDH activity. However, CdCl2 (1.0 mmol·L−1) causes high inhibitory effect on the enzyme activity. To explore the role of G6PDH on the enhancement of freezing resistance
induced by freezing acclimation, the changes in the cytosolic G6PDH activity and freezing resistance (expressed as LT50) of P. suaveolens cuttings during freezing acclimation at −20 °C were investigated. The results reveal that freezing acclimation decreases
LT50 of cuttings, and increases the activity of cytosolic G6PDH compared with control ones, while 2 d of de-acclimation at 25
°C result in a decrease in cytosolic G6PDH activity, and caused an increase in LT50. Furthermore, the change in cytosolic G6PDH activity is found to be closely correlated to the degree of freezing resistance
of cuttings during freezing acclimation. It is suggested that cytosolic G6PDH may be involved in the induction of freezing
resistance of cuttings.
[Supported by the Foundation of State-designated Base for Biology Researching and Teaching in Beijing Forestry University] 相似文献
16.
Cold acclimation is associated with many metabolic changes that lead to an increase of freezing tolerance. In order to investigate the biochemical process of cold acclimation in Ammopiptanthus mongolicus, seedlings were acclimated at 2℃ under 16-h photoperiod （150 μmol·m^-2·s^-1 photosynthetically active radiation） for 14 d. Freezing tolerance in seedlings increased after 14 d of cold-hardening. Contents of protein, proline and solute carbohydrate in cotyledon increased after cold acclimation. Patterns of isozymes of superoxide dismutase （SOD）, peroxidase, catalase and polyphenol oxidase （PPO） were investigated. The activities of SOD, peroxidase and PPO in cold acclimated plants were increased during cold-hardening. We deduced that compatible solutes and antioxidant enzymes play important roles in development of freezing tolerance during cold acclimation in this evergreen woody plant. 相似文献