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1.
Cornus mas L. is a naturally growing dogwood species in Anatolia. In present study, physical, chemical and antioxidant properties of cornelian cherry fruits were studied. The fruit weight was in the range of 0.39–1.03 g, fruit length 14.24–22.20 mm, fruit width 9.59–13.21 mm, flesh/seed ratio 1.34 to 6.72. Hunter L values of the samples ranged between 10.82 and 19.69, and a value was between +6.25 and +15.59, and b value was between +3.46 and +6.64. In addition to the levels of dry matter, soluble solids, pH, total acidity, total sugar content, reduced sugar content, unreduced sugar content, ascorbic acid, total anthocyanin and total phenolics were within the range of 15.88–28.19%, 12.50–21.00%, 3.11–3.53, 1.10–2.53%, 76.80–154.00 g kg−1, 52.80–120.00 g kg−1, 0.00–32.30 g kg−1, 0.16–0.88 mg g−1, 1.12–2.92 mg g−1 and 2.81–5.79 mg g−1, respectively. On the other hand, ferric reducing antioxidant power (FRAP) and EC50 values were between 16.21 mmol g−1 and 94.43 mmol g−1, 0.29–0.69 mg mL−1. Anthocyanin extracts of the fruits were analysed by high-performance liquid chromatography (HPLC) with UV–vis detection. Pelargonidin 3-glucoside was the main pigment found in cornelian cherry fruits.  相似文献   

2.
Stands of summer cauliflower were grown within polyethylene-covered tunnels along which a temperature gradient was imposed. Two tunnels were maintained at either normal or elevated CO2 concentrations. At the last harvest (88 days from transplanting) no interaction between CO2 and temperature on total biomass was detected. The total dry weight of plants grown at 531 μmol mol−1 CO2 was 34% greater than those grown at 328 μmol mol−1 CO2, whereas a 1 °C rise reduced dry weight by 6%. From serial harvests the radiation conversion coefficient was 2.01 g MJ−1 and 1.42 g MJ−1 at 531 μmol mol−1 CO2and 328 μmol mol−1 CO2, respectively, but was not greatly affected by differences in temperature. No effect of either CO2 or temperature on the canopy light extinction coefficient was detected. The rate of progress towards curd initiation increased to a maximum at 15.5 °C, and declined thereafter. Provided the effect of temperature was accounted for, CO2 enrichment did not affect the time of curd initiation. From serial harvests after curd initiation, the logarithm of curd weight or diameter were negative linear functions of mean temperature from initiation. Increases in curd weight and diameter at 531 compared with 328 μmol mol−1 CO2 were greater at warmer temperatures (27% at 13 °C compared with 47% at 15 °C, 57 days after initiation). Effects of CO2 on curd diameter were less than those on curd dry weight because the curd dry matter content was greater at 531 compared with 328 μmol mol−1 CO2. Thus, the effects of elevated CO2 concentrations on fresh weight based yield parameters of cauliflower were less than the increase in total dry matter production.  相似文献   

3.
The Alstroemeria cultivars Diamond, King Cardinal and Libelle were grown for 18 months under five lighting regimes with, and without, soil cooling. The aim was to optimize the daily investment of light energy from artificial sources with respect to photoperiod and photosynthetic fluence rates and to elucidate possible links between reactions to photoperiod and root-zone temperature. The more photons (photosynthetically active radiation, PAR) that were supplied to the plants per day (8, 11 and 13 mol m−2), the higher was the total production of flowering stems. The total yield from regimes with 13 mol m−2 day−1 was higher when the light was spread over 20 and 16 h compared to 12 h. In treatments with soil cooling, the plants flowered continuously under all combinations of photoperiods and photosynthetic fluence rates, and the summer and autumn recession in flower production that occurred for non-cooled ‘King Cardinal' and ‘Diamond' was the same under all lighting regimes. It is concluded that it might be more cost-effective to spread the daily investment of light over 20 rather than 16 or 12 h when the total energy budget and CO2 costs are taken into consideration.  相似文献   

4.
The present work differentiates the effects of NaCl, Cl−, Na+ and concentrated macronutrients on two citrus species, sour orange (Citrus aurantium L) and Macrophylla (Citrus macrophylla Wester). Plants were grown in a base nutrient solution (0.07 MPa osmotic pressure) for 4 months before applying the treatments that consisted of isotonic solutions of 0.23 MPa osmotic pressure of Na+ (40 mM, without Cl−), Cl− (40 mM, without Na+), NaCl (40 mM) and 3.5 times the concentration of macronutrients of the base solution. Plants were grown in the different treatment for 2 months before being examined for symptoms of toxicity. The two genotypes showed major differences in the extent of Cl− and Na+ accumulation in leaves and in their ability to maintain the internal concentrations of essential nutrients in response to the different ionic compositions of the medium. Differences in mineral nutrient accumulation were observed among treatments in both rootstocks. It was concluded that growth response to the different treatments was primarily affected by an osmotic effect, although in Macrophylla, the ionic effects also seem to be present.  相似文献   

5.
为改善冀西北森林水土保持和水源涵养的能力,在河北省张家口市涿鹿县林场选择油松、华北落叶松、山杨、白桦4种林分类型,以撂荒地作为对照,进行了土壤入渗和贮水特征的研究。结果表明,土壤饱和贮水量排序为：白桦林(373.24 mm)>油松林(352.72 mm)>山杨林(325.20 mm)>华北落叶松林(306.28 mm)>撂荒地(286.30 mm)。土壤容重均值排序为：白桦林(0.90 g/cm3)<油松林(0.95 g/cm3)<山杨林(0.99 g/cm3)<华北落叶松林(1.00 g/cm3)<撂荒地(1.27 g/cm3)。土壤入渗速率与入渗时间呈现明显幂函数关系,林分的土壤渗透性能均高于撂荒地,其中白桦林稳渗速率最大(5.21 mm/min),水土保持能力最好。土壤容重与土壤孔隙度、贮水量呈负相关关系。森林对非毛管孔隙(滞留贮水)的改善作用要大于毛管孔隙(吸持贮水)的改善作用。  相似文献   

6.
以河北省黄土梁子林场油松林为研究对象,设置4种不同经营措施(抚育间伐、天然更新幼林抚育、抚育+林冠下造林、更新采伐)人工林样地,采用室内浸水法和环刀浸泡法,对样地内枯落物的现存量、持水量以及土壤物理性质等进行测定,并对其水源涵养能力进行研究,以期为冀北山地油松林分的森林经营提供依据。结果表明,枯落物现存量变动范围在8.02～15.90 t/hm2,由大到小依次为：更新采伐>抚育间伐>天然更新幼林抚育>抚育+林冠下造林;更新采伐下枯落物最大持水量最高,为47.72 t/hm2,最大持水率最高,为219.10%;土壤容重均值变化范围在1.00～1.39 g/cm3,其中抚育+林冠下造林土壤容重最大为1.39 g/cm3,抚育间伐土壤容重最小为1.00 g/cm3;土壤总孔隙度变动范围在25.72%～61.41%,由大到小依次为：更新采伐>抚育间伐>天然更新幼林抚育>抚育+林冠下造林;最大持水量变动范围在273.56～896.21 t/hm  相似文献   

7.
AIM: Gene transduction with a recombined murine stem cell retroviral vector has been investigated to find an effective way of gene transduction and to offer theory and experimental basis for the recombined murine stem cell retroviral vector used for gene transduction. METHODS: 1. Construction of retrovirus vector: EC1-4 gene (repeats 1-4 of cadher in-5 extracellular domain) and mutant (Ser 222A) MEK1 gene were cloned into retrovirus vector pMSCV after cut by Bgl Ⅱ and EcoR 1 restriction endonuclease. 2. Obtaining CD41+ cells and cell culture: Cells expressing CD34+ from cord blood were isolated. The inducement of cells expressing CD41 from CD34+ cells was performed by using TPO and cells CD41+ were selected by FACS. NIH 3T3 cells were cultured in high sugar DMEM medium and U937 in RPMI 1640 medium. UT7 cells which is cytokine-dependent cell line were grown in Iscove's modified Dulbeco's medium supplemented by GM-CSF. 3. Determination of viral titers: Retroviral vectors were transferred to packaging cell line 293. Retroviral containing supenatant was collected after transfection. The viral titers was tested on infection of NIH 3T3 cells by FACS analysis. 4. Western blot: Transduced CD41+, UT7, U937 and MDA-MB-435 cells were analyzed by western blot to examine expression of transduced genes. RESULTS: A packaging cell line 293 produces high-titer MEK1 pMSCV retroviruses (3.1×107) and EC1-4 pMSCV retroviruses (1.0×108). With 8-folds dilution retroviruses, 60.73% GFP positive cells have been obtained in MEK1 pMSCV transduced UT7 cells, 72.56% in U937 cells and 30.57% in CD41+ cells, respectively. GFP positive cells have reached up 97.54 % in EC1-4 pMSCV transducted MDA-MB-435 cells. Phosphorylated MEK1 has been decreased in experiment group when TPO has stimulated CD41+ and UT7 cells or serum has stimulated U973 cells. This indicates that is a dominant negative effect of mutation MEK gene. EC1-4 gene transduced MDA-MB-435 cells have expressed EC1-4. CONCLUSION:The recombined murine stem cell retrovirus can effectively mediate gene transduction of CD41+,UT7,U937 and MDA-MB-435 cells,and transduced genes can be stably expressed.  相似文献   

8.
In arid and semi-arid regions, where water availability is a major limitation in crop production, using alternative water resources, such as saline water is one way to utilize lands. Chamomile (Matricaria recutita L.) as an annual medicinal herb may be considered as an economic substitute for field crops irrigated with fresh water since it has adaptability to wide range of climate and soil. A field examination was conducted during 2004–2005 using complete randomized block design with four replications in order to evaluate the effects of saline irrigation water on morphological characters, mineral content, oil quantity (content, yield), oil composition and apigenin content of chamomile. In each plot, 0.6 g/m2 of seeds were grown in 4 rows. The irrigation water had five different salinity levels (0, 4, 8, 12 and 16 dS m−1). The investigated characters through cultivation were fresh weight of flower (g), dry weight of flower (g), dry weight of aerial stems (g), dry weight of root (g), oil yield (kg/h), oil content (%), oil quality and apigenin content (%). After harvesting, the content of minerals (Na+, Cl−, K+, Ca2+, Mg2+) were evaluated in aerial parts and roots of each plot. Mean comparisons for fresh flower weight in different treatments showed that fresh flower yield decreased with increasing salinity and it was higher in control compared to others. Analysis of variance showed that saline irrigation water had no significant effect on oil quantity (yield and content), oil quality (chemical composition) or apigenin content. Our results showed that chamomile is able to maintain all its medical properties, under saline condition and could be cultivated economically in such conditions.  相似文献   

9.
Three-month-old mist-rooted ‘Picual’ olive cuttings were transplanted into 2-l plastics pots containing perlite as substrate and fertigated with a complete nutrient solution containing 0.05, 0.1 or 2.5 mM KCl depending on the experiment. In the first experiment, plants were sprayed with RbCl (Rb+ is a K+ analog) at a rate of 4% at 63 days after transplanting (DAT). Foliar Rb+ uptake through leaves increased with K+ concentration in the nutrient solution, indicating that foliar Rb+ uptake was lower when plants were K+ deficient than when they were adequate. On the contrary, it was observed that translocation of Rb+ from leaves to other organs of the plant was higher under K+ deficiency conditions. In the second and third experiments, when differences appeared on shoot length due to K+ nutritional status (0.05 or 2.5 mM KCl) at 63 DAT, a group of plants were subjected to water stress during 7 weeks. On the other hand, another group of plants (control plants) did not receive any water stress treatment during the experiments. After this period of 7 weeks, all plants were sprayed with RbCl at 4%. Leaf K+ concentration diminished in water-stressed plants independently of plants nutritional status. Foliar Rb+ uptake through leaves was restricted by water stress either in plants with low K+ (0.05 mM KCl) or plants with high K+ (2.5 mM KCl). Translocation of Rb+ from leaves was greater under water stress conditions in both K+ nutritional statuses. In conclusion, the results obtained could explain the irregular response in olive trees to foliar K+ sprays, particularly, when they grow in rainfed orchards.  相似文献   

10.
AIM To explore an effect and high growth rate of primary culture method for keratinocytes from the back skin of adult mice, and to compare the proliferation, colony formation and apoptosis of keratinocytes from the back skin of neonatal mice and adult mice. METHODS The back skin of adult mice was digested by 4 different methods. The keratinocytes were isolated and cultured from the epidermis, and were identified by immunofluorescence staining. The density and the pattern of seed plates were adjusted. CCK-8 assay, EdU assay, crystal violet staining and TUNEL method were used to detect the proliferation, colony formation and apoptosis of the keratinocytes from the back skin of neonatal and adult mice. RESULTS Among the 4 different methods of epidermal-dermal isolation in the adult mice, the two-step digestion method had the largest number, the strongest cell proliferation ability and the highest efficiency of colony formation of cells. The water-drop seed plate method had a higher rate of colony formation than the common seed plate method. The optimum density of keratinocytes in neonatal mice was about 3.2×104/cm2, and the optimum density of keratinocytes in adult mice was about 1.6×104/cm2. Compared with the adult mouse, the neonatal mouse back skin keratinocytes had stronger proliferation ability and higher colony formation efficiency. CONCLUSION A primary culture system of keratinocytes from the back skin of adult mice has been successfully established.  相似文献   

11.
AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFbSHR) and WKY (CFbWKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and[3H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm2 of FN. Collagen synthesis was determined by [3H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×104 cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in [3H]-proline incorporation in both CFbSHR and CFbWKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY.  相似文献   

12.
AIM:To establish the method for detecting the immunophenotype of immunosuppressive receptor programmed cell death protein 1 (PD-1) in T-cell receptor (TCR) Vβ repertoire of CD3+, CD4+ and CD8+ T-cell subsets, therefore to evaluate the distribution of PD-1 in T-cell repertoire from human peripheral blood (PB). METHODS:The PB samples from 10 cases of healthy individuals (HI) were collected. Using multi-colored fluorescence flow cytometry, the distribution frequency of PD-1 in TCR Vβ repertoire was detected with a wide panel of anti-CD45, anti-CD3, anti-CD4, anti-CD8, anti-PD-1 and 24 anti-TCR Vβ repertoire (IOTest® Beta Mark TCR Vβ Repertoire Kit, containing 8 tubes which labeled A~H, each tube is a composite antibody of FITC and PE coupling, each cocktail contains antibodies direc-ted to 3 different Vβ subfamilies) monoclonal antibodies. RESULTS:The total number of the 24 TCR Vβ repertoire detected in CD3+, CD3+CD4+ and CD3+CD8+ T cells from 10 cases of HI was consistent with the Quick Reference Card data provided by the kit. The preliminary results showed that the frequency of Vβ usage in CD3+, CD4+ and CD8+ T cells was different. High usage of Vβ2, Vβ3, Vβ8, Vβ9, Vβ5.1, Vβ13.1 and Vβ13.2 was found in CD3+ T cells, while high usage of Vβ2, Vβ3, Vβ8, Vβ5.1, Vβ9 and Vβ13.1 in CD3+CD4+ T cells, and high usage of Vβ1, Vβ2, Vβ3, Vβ9, Vβ13.1 and Vβ13.2 in CD3+CD8+ T cells were also observed. Further analysis showed that the expression of PD-1 was detected in all 24 TCR Vβ subfamilies of CD3+, CD3+CD4+ and CD3+CD8+ T cells. The higher frequency of PD-1+ T cells was CD4+Vβ5.2+ T cells, whereas the higher frequency of PD1+ T cells in CD8+Vβ11+ and CD8+Vβ13.6+ T cells was detected. CONCLUSION:The method for detection of the immunosuppressive receptor PD-1 in TCR Vβ repertoire of T-cell subsets is successfully established, which provides a new method for further analysis of immunosuppressive characteristics of TCR Vβ repertoire in the patients with leukemia.  相似文献   

13.
AIM: To investigate the effects of integrin-linked kinase (ILK) overexpression on survival and proliferation of cardiac c-Kit+ cells, and the role of ILK-overexpressing c-Kit+ cell transplantation in cardiac function in a rat myocardial infarction (MI) model.METHODS: Cardiac c-Kit+ cells were isolated from the hearts of neonatal Sprague-Dawley (SD) rats and cultured to prepare the ILK-c-Kit+ cells by infected with recombinant adenoviral vector harboring human wild-type ILK cDNA. The survival and proliferation of cardiac c-Kit+ cells were detected by cell counting and CCK-8 assay at 48 h after infection, respectively. The protein levels of cyclin D1 and proliferating cell nuclear antigen (PCNA) in the cardiac c-Kit+ cells were examined by Western blot. MI was induced by coronary artery ligation in 40 adult rats. After 15 min, ILK-c-Kit+ cells were transplanted into the hearts by myocardial injection at 3 different sites in the infracted zone and border zone. All rats were randomly divided into 4 groups:sham group, MI plus saline injection group (MI group), MI plus null vector-infected cardiac c-Kit+ cell injection group (Ad-null-c-Kit+ cell group), and MI plus ILK-overexpressing cardiac c-Kit+ cells injection group (ILK-c-Kit+ cell group), with 10 rats in each group. At 2 weeks after MI, the protein levels of c-Kit in MI hearts were investigated by immunohistochemical assay. At 4 weeks, left ventricular function was examined by hemodynamic measurement.RESULTS: The survival and proliferation of cardiac c-Kit+ cells and the protein levels of cyclin D1 and PCNA were enhanced by ILK overexpression compared with Ad-null group. In MI rat model, the number of c-Kit+ cells was increased by ILK-c-Kit+ cell injection compared with Ad-null-c-Kit+ cell group at 2 weeks after MI. Cardiac function was significantly improved in ILK-c-Kit+ cell-transplanted rats.CONCLUSION: ILK overexpression improves survival and proliferation of cardiac c-Kit+ cells by increasing the protein levels of cyclin D1 and PCNA. ILK-c-Kit+ cell transplantation enhances the therapeutic efficiency of cardiac c-Kit+ cells in the post-MI hearts of rats.  相似文献   

14.
AIM: To study the effects of cyproheptadine (Cyp) and anisodamine (Ani) on the changes of intracellular free Ca2+ concentration ([Ca2+]i) induced by tumor necrosis factor (TNFα) in single endothelial cells, and to explore the mechanisms of TNFα mediated shock and antishock actions of Cyp and Ani. METHODS: Human umbilical vein endothelial cell strains (ECV304) were seed in 35 mm tissue culture dish with 2 mL DMEM culture medium. The cultured cells were loaded by Fluo-3/AM. The spatial distribution and the dynamic changes of [Ca2+]i in single endothelial cell was determined by laser scanning confocal microscopy (LSCM). RESULTS: [Ca2+]i in single endothelial cell after stimulation of TNFα rapidly increased in a dose-dependent manner and approached the peak value within 60 seconds, afterwards, decreased and kept above the basal level. The confocal scanning image showed that [Ca2+]i elevation was more obvious in nuclear than in cytoplasma, and decreased slowly. Cyp (3×10-5, 6×10-5 mol/L) and Ani (2×10-5, 4×10-5 mol·L-1) markedly inhibited TNFα (1.2×10-9 mol·L-1)-induced [Ca2+]i elevation. CONCLUSIONS: TNFα markedly induces elevation of [Ca2+]i in single endothelial cell, it may be an important mechanism of TNFα-induced shock and tissue injury. Cyp and Ani obviously suppress TNFα-induced [Ca2+]i elevation, which probably is one of the mechanisms of their antishock effects.  相似文献   

15.
AIM:To investigate the effect of glucocorticoids, a kind of traditional immunosuppressive drug, on expanding central memory CD8+ T cells (CD8+ TCM) and to provide a novel method of generating CD8+ TCM for adoptive immunotherapy.METHODS:Healthy human peripheral blood mononuclear cells were isolated by density gradient centri-fugation. Naïve CD8+ T cells were further isolated with MACS microbeads and flow cytometry. After activated by cytokines, the cells were divided into experimental group and control group. Glucocorticoids at different concentrations and an identical volume of PBS were added. The phenotypic characteristics of TCM in different groups were measured by flow cyto-metry at separate time points. Furthermore, the effect of glucocorticoids on CD8+ T cell expansion was further verified using CFSE assay and Annexin V staining.RESULTS:Glucocorticoids significantly increased the proportion of CD8+ TCM in vitro. Glucocorticoids sustained CD8+ T cell expansion. Glucocorticoids had low toxicity for CD8+ T cells.CONCLUSION:Glucocorticoids effectively increase the number and proportion of CD8+ TCM in vitro, which provides novel insights into the generation of human CD8+ TCM and reveals a novel potential clinical application of glucocorticoids for immunotherapy.  相似文献   

16.
AIM: To investigate the cellular biological effects of matrine on K562 and K562/Vin cells and discuss the anticancer mechanism of matrine. METHODS: MTT assay was used to detect the IC50 of matrine on these two cell lines and the reversal effect of matrine on K562/Vin cell's resistance to vincristine. In addition, the growth curve of cells was drawed. The p-glycoprotein (P-gp) expression was determined by immunohistochemistry analysis. The morphological changes of cells under light microscopy and the structural changes under transmission electron microscope were observed. RT-PCR assay was used to detect the hTERT-mRNA expression. RESULTS: The IC50 of matrine was 3.4, 4.6 mmol·L -1 for K562 and K562/Vin cells, respectively. Matrine (4.0 mmol·L -1) inhibited the growth of K562, K562/Vin cells, 2.0 mmol·L -1 matrine inhibited expression of P-gp and with 492.4 reversal index. Matrine killed K562 cells by inducing the apoptosis and the same effect on K562/Vin cells was also observed. The hTERT-mRNA expression of K562 cells were also inhibited by matrine. CONCLUSIONS: Matrine enhanced the cytotoxicity of vincristine in K562/Vin cells, induced the apoptosis of K562 and K562/Vin cells, also inhibited the hTERT-mRNA expression in K562 cells. It shows that matrine would be an effective anticancer medicine.  相似文献   

17.
丁云花 《园艺学报》2016,43(Z2):2735-2736
‘京松1号’是以细胞质雄性不育系CMS060为母本,自交系1028为父本配制而成的松散型花椰菜一代杂种。植株生长势强,中熟,秋季从定植至收获85 d左右,春季从定植至收获65 d左右。花球白、松散,花梗浅绿,单球质量1.5 kg左右,维生素C含量515 mg ? kg-1 FW,蛋白质含量2.3%,可溶性糖含量2.05%,钾含量2 761.0 mg ? kg-1 FW。适合我国北方地区春秋季栽培。  相似文献   

18.
AIM: To explore the effect of microRNA (miR)-21 on proliferation, migration and differentiation abilities of c-Kit+ cardiac stem cells (CSCs). METHODS: c-Kit+ CSCs were cultured and selected by the methods of enzyme digestion and magnetic bead separation. miR-21 mimics (50 nmol/L) and mimics negative control (MNC) were transfected into c-Kit+ CSCs with Lipofectamine® 2000. The cells was divided into 3 groups:control group:c-Kit+ CSCs without any pretreatment; MNC group:the cells were transfected with MNC for 48 h; mimics group:the cells were transfected with miR-21 mimics for 48 h. qPCR was used to assess the expression of miR-21 in each group. CCK-8 and EdU assays were used to determine the cell proliferation. qPCR and immunofluorescence were used to detect the differentiation in each group. Scratch assay was adopted to explore the migration ability of the cells. RESULTS: The expression of c-Kit in the c-Kit+ CSCs were 90.8%, with 0.6% of CD45 and 0.5% of CD34. A significant increase in miR-21 expression was observed when the cells were transfected with miR-21 mimics for 48 h (P<0.05). CCK-8 and EdU assays showed that miR-21 significantly increased cell proliferation as compared with MNC group and control group (P<0.05). No difference in the expression of Nkx2.5, CD31 and α-SMA at mRNA and protein levels was observed, and no difference of the migration ability in 3 groups of the c-Kit+ CSCs was found. CONCLUSION: Over-expression of miR-21 significantly promotes the proliferation of c-Kit+ CSCs, without any effect on the cell migration and differentiation.  相似文献   

19.
AIM: To investigate the role of imperatorin in reversing the resistance of the PC9 CD133+ cell subsets to gefitinib. METHODS: MTT assay was performed to evaluate the viability of PC9 cells treated with imperatorin and gefitinib. The expression of c-met, activation of caspases and phosphorylation of epidermal growth factor receptor (EGFR), PI3K and AKT in the PC9 cells treated with imperatorin and gefitinib were determined by Western blot. The percentage of CD133+ cell subsets population and the apoptotic rate of the PC9 cells treated with imperatorin and gefitinib were analyzed by flow cytometry. RESULTS: The sensitivity of the PC9 CD133+ cell subsets to gefitinib was significantly lower than that of the PC9 CD133- cell subsets. Treatment with gefitinib alone significantly inhibited the protein levels of EGFR/PI3K/AKT in the PC9 CD133- cell subsets but not the PC9 CD133+ cell subsets. Treatment with gefitinib alone increased the percentage of CD133+ cell subsets population in the PC9 cells. However, combination of gefitinib with imperatorin significantly inhibited the enrichment of CD133+ cell subsets population. Imperatorin down-regulated c-met expression, suggesting the c-met was the target of imperatorin in the PC9 CD133+ cell subsets. The results of MTT assay, Western blot analysis and flow cytometry indicated that imperatorin increased the gefitinib induced inhibition of PI3K/AKT protein levels by down-regulating the expression of c-met, which subsequently induced the cleavage of caspases and apoptosis in the PC9 CD133+ cell subsets.CONCLUSION: Imperatorin increases the sensitivity of lung cancer CD133+ cell subsets to gefitinib by down-regulating the expression of c-met, and the synergistic anti-tumor effect exists between imperatorin and gefitinib.  相似文献   

20.
AIM: To investigate the role of reactive oxygen species (ROS) and calcium overload in the apoptosis of MC3T3-E1 cells induced by high glucose. METHODS: Cultured mouse skull bone-derived osteoblast cell line MC3T3-E1 was treated with high concentration of D-glucose to induce apoptosis. The proliferation of MC3T3-E1 cells was detected by MTT assay after treated with different concentrations of D-glucose for 24 h and 48 h. The apoptotic rate and the intracellular levels of calcium and ROS were also measured after the cells were treated with high glucose (35 mmol/L) for 24 h. RESULTS: After high glucose treatment, the cell proliferation was inhibited. The early apoptosis and total cell death increased to (24.16?3.53)% and (63.74?4.32)%,respectively. High glucose treatment significantly increased intracellular levels of ROS and Ca2+. The increased apoptotic rate was reduced by addition of antioxidant N-acetylcysteine and calcium chelator BAPTA-AM. Inhibition of store-operated Ca2+ channels by La3+ also decreased the intracellular level of Ca2+ and cell apoptosis induced by high glucose. CONCLUSION: High glucose increases intracellular ROS level and the release of Ca2+ through the store-operated Ca2+ channels, thus resulting in intracellular Ca2+ overload and leading to apoptosis of osteoblasts.  相似文献   

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