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南美白对虾对虾杆状病毒病的诊断与防治 总被引：1，自引：0，他引：1
本文对对虾杆状病毒病的病原、流行情况、症状、诊断作了综述,对南美白对虾感染对虾杆状病毒病的预防提出了实用措施,对其治疗提出了原则措施. 相似文献
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一、养殖虾类肝胰腺症（病）的非寄生性病因及其控制问题 近年来，不管是罗氏沼虾、刀额新对虾等的淡水池塘养殖，还是中国对虾、斑节对虾等的海水池塘养殖，原发性肝胰腺病或肝胰腺病变和坏死症状均较严重，有的成为继发性细菌病暴发的条件，有的成为继发性病毒病的侵入条件，还有的继续恶化为严重的非寄生性肝胰腺病。对于病原性细菌病和病毒病等多种疾病的病理学研究表明，肝胰腺病变或坏死的症状均是普遍和严重的，这应该归罪于致病病原生物的毒素作用。而非病原性肝胰腺病的致病原因则是多方面的、复杂的，有时难以检验和诊断。随着… 相似文献
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对虾病毒性疾病有多种，如白斑病毒、桃拉病毒、黄头病毒等。多年来，科研人员对病毒病的诊断方法进行了大量的研究，现代生物检测技术得到较多应用，如酶联免疫吸附（ELISA）、聚合酶链式反应（PCR）、核酸探针斑点杂交等，发表了较多提取病毒、改进病毒检测方法等文章。检测方法越来越简易、灵敏，并开发有WSSV、TSV等病毒检测试剂盒。利用现代生物检测技术诊断对虾病毒病对监测养殖生产中的疾病流行趋势有一定的作用，但各种检测方法大都局限于国家机构的实验室进行，难以大规模商业化应用。是什么原因使先进的检测技术无法服务于养殖生产？现结合生产以及科研实践进行多方面探讨，供参考。 相似文献
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国内外对虾病毒病研究综述 总被引：3，自引：0，他引：3
本文就对虾病毒最新进展与动态进行了概述。具体介绍了对虾病毒研究的各项成果,列出了已见报道的近20种对虾病毒病原的中英文名称;描述它们的生物学和病理学的一些特征,包括对虾病毒的地理分布、对虾病毒的传播途径、感染病对虾的发病症状以及宿主虾细胞、组织和器官的病理变化;概述了对虾病毒的诊断方法:显微镜观察生物学测定、免疫学方法、细胞培养、生化检验、分子生物学方法;根据目前还未发现有效治疗对虾病毒病的药物现状,指出了几种预防对虾病毒病的措施。 相似文献
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《中国水产》2013,(2):40-40
1月，由中国水产科学研究院黄海水产研究所黄健研究员等以及多个单位联合发明的“检测多种鱼类病原的基因芯片及其检测方法”获国家发明专利授权，专利号为ZL201010243398．3。由黄健研究员等发明的“检测多种对虾病原的基因芯片及其检测方法”获国家发明专利授权，专利号为ZL201010243399．3。前项发明涉及一种检测多种鱼类病原的基因芯片及其检测方法。17种鱼类病原的探针包括：用于检测14种病毒的探针，用于检测淀粉卵涡边虫和三代虫的探针以及用于检测鱼立克次氏体的探针。后项发明涉及一种检测多种对虾病原的基因芯片及其检测方法。包含的18种对虾病原的探针为：用于检测15种病毒的探针，用于检测镰刀菌、杀鱼丝囊霉菌、变形藻丝囊菌的探针以及检测拟阿脑虫和对虾立克次氏体的探针。 相似文献
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对虾是我国水产经济动物的一大品种,随着人工养殖时间的延长,对虾自身抗病力减弱,环境污染,病原体较多,对虾病害已成为威胁对虾养殖业的一个严重问题。多年来,我们对养殖对虾主要疾病的病原及防治方法进行了深入的研究,在养殖过程中建立了严格的消毒措施,控制病毒的传播途径，建立了实用、灵敏、准确的病原监测程序，推广使用快速、灵敏的对虾病毒检测技术（PCR检测方法及核酸探针检测方法）， 相似文献
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1994年浙江省对虾暴发性流行病病原及传播途径的初步调查 总被引：4，自引：0，他引：4
用Ｔ—Ｅ染色、单克隆抗体的ＥＬＩＳＡ现场诊断、组织切片和Ｈ—Ｅ染色和电镜观察等不同方法对１９９４年浙江省乐清和温岭两地区虾池及海区样品进行了现场和实验室检查。结果说明，１９９４年浙江省对虾暴发性流行病实质上是杆状病毒性的皮下及造血组织坏死，病原为ＨＨＮＢＶ。检查到的ＨＰＶ不是这场暴发病的病原。对非对虾类生物和对虾鲜活饲料的单抗ＥＬＩＳＡ检测表明，海区张网饲料可能是１９９４年浙江对虾暴发性流行病的主要传染源，病虾池中除对虾外，其它甲壳类生物在该病的传播上可能也起到一定的作用。这些生物除了可能传播ＨＨＮＢＶ外，还可能传播ＨＰＶ。 相似文献
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Thang Nguyen Dinh Tu Le Dinh Le Na Nguyen Thi Trang Ngo Thi Nghia Phan Tuan 《International Aquatic Research》2019,11(3):303-310
International Aquatic Research - Viral diseases are a serious issue for the shrimp aquaculture industry. White spot syndrome virus (WSSV) has been considered one of the most dangerous pathogens... 相似文献
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Fish and shellfish diseases are a constant threat to the sustainability and economic viability of aquaculture. Early diagnosis plays a vital role in management of fish and shellfish diseases. Traditionally, various biochemical and serological tests have been used for fish disease diagnosis. However, the time and expertise required for such diagnoses makes it difficult for aquaculturists to easily adopt them under production conditions. Polymerase chain reaction and probe-based nucleic acid detection have become increasingly popular in fish and shellfish diagnostics. Recently, a novel technique called loop-mediated isothermal amplification (LAMP) has been developed, which is highly sensitive and rapid. LAMP has been used for the detection of bacterial, viral, fungal and parasitic diseases in both animal and plants. In aquaculture, LAMP-based detection of pathogens like Edwardsiella tarda, E. ictaluri, Nocardia seriolae, Tetracapsuloides bryosalmonae, white spot syndrome virus and infectious haematopoietic necrosis virus have been reported. In this review, the application of LAMP for the detection of aquaculture-associated pathogens is discussed. 相似文献
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传染性皮下及造血组织坏死病毒(IHHNV)是一种分布较广、危害较大的对虾病毒,已被世界动物卫生组织(OIE)列为须向其申报的甲壳类重要疫病病原。IHHNV在我国已形成了一定的流行趋势,目前仍是严重危害我国养殖虾类的重要病毒。本文从IHHNV的流行地区及危害、宿主、致病类型、对宿主不同年龄阶段的致病性差异、与白斑综合征病毒(WSSV)的干扰作用、感染机制和致病机理方面,综述了与IHHNV致病性相关的研究进展,以期为进一步深入研究IHHNV防控提供参考资料。 相似文献
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特种水产养殖动物病毒病原的研究现状 总被引：2，自引：0，他引：2
本文举例阐明了病毒(及类似病毒)病原引起的突发性传染病给我国特种水产养殖业带来的重大损失和潜在威胁;分别对国内外爬行动物(包括鳖)、两栖动物(包括蛙)、虾、鳗等特种水产养殖动物病毒病原研究的情况进行了概述;将已知的病毒种类和中英文名称,按不同宿主归类列出;并根据最新资料以及有关工作进展情况,对上述几种特种水产养殖动物病毒病原研究的动态作了简介。 相似文献
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江苏地区对虾3种病毒病的流行病学调查及5株IHHNV的编码区基因序列分析 总被引：1，自引：1，他引：0
对虾白斑综合征病毒(WSSV)、桃拉综合征病毒(TSV)、传染性皮下及造血组织坏死病毒(IHHNV)是威胁对虾养殖的重要病毒。为了调查这3种病在江苏境内的流行情况,根据世界动物卫生组织(OIE)推荐的《水生动物疾病诊断手册》的PCR检测法对2015年5月—2016年5月采集的1436尾对虾样品进行3种疾病的流行病学调查。结果显示WSSV阳性率为17.20%,TSV阳性率为0%,IHHNV阳性率为39.48%。对江苏不同地区分离出的5株IHHNV进行基因序列分析,数据显示这5个地区的毒株均属于Ⅰ型感染株,与韩国株的进化关系较为接近。本研究通过调查以上3种病毒病在江苏境内的流行情况,并进行序列分析,发现江苏不同地区的凡纳滨对虾IHHNV感染均为Ⅰ型,研究结果对养殖虾的疾病防控有重要参考价值。 相似文献
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We produced a panel of monoclonal antibodies (MAbs) from the fusion of Taura syndrome virus variants from Belize (TSV-BZ) immunized BALB/cJ mouse spleen cells and non-immunoglobulin secreting SP2/0 mouse myeloma cells. One antibody, 2C4, showed strong specificity and sensitivity for TSV in dot-blot immunoassay and immunohistochemistry (IHC) analysis. The MAb reacted against native TSV-BZ, TSV variants from Sinaloa, Mexico (TSV-SI) and TSV variants from Hawaii (TSV-HI) in dot-blot immunoassay. By IHC, the antibody identified the virus in a pattern similar to the digoxigenin-labelled TSV-cDNA probe for the TSV-BZ, TSV-HI and TSV-SI variants, but not for the TSV variants from Venezuela (TSV-VE) and the TSV variants from Thailand (TSV-TH). MAb 2C4 did not react against other shrimp pathogens or with normal shrimp tissue. Western blot analysis showed a strong reaction against CP2, a region of high antigenic variability amongst TSV variants. This antibody has potential diagnostic application in detection and differentiation of certain TSV biotypes. 相似文献
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A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus 总被引：5，自引：0，他引：5
Escobedo-Bonilla CM Alday-Sanz V Wille M Sorgeloos P Pensaert MB Nauwynck HJ 《Journal of fish diseases》2008,31(1):1-18
Since it first appeared in 1992, white spot syndrome virus (WSSV) has become the most threatening infectious agent in shrimp aquaculture. Within a decade, this pathogen has spread to all the main shrimp farming areas and has caused enormous economic losses amounting to more than seven billion US dollars. At present, biosecurity methods used to exclude pathogens in shrimp farms include disinfecting ponds and water, preventing the entrance of animals that may carry infectious agents and stocking ponds with specific pathogen-free post-larvae. The combination of these practices increases biosecurity in shrimp farming facilities and may contribute to reduce the risk of a WSSV outbreak. Although several control methods have shown some efficacy against WSSV under experimental conditions, no therapeutic products or strategies are available to effectively control WSSV in the field. Furthermore, differences in virulence and clinical outcome of WSSV infections have been reported. The sequencing and characterization of different strains of WSSV has begun to determine aspects of its biology, virulence and pathogenesis. Knowledge on these aspects is critical for developing effective control methods. The aim of this review is to present an update of the knowledge generated so far on different aspects of WSSV organization, morphogenesis, pathology and pathogenesis. 相似文献
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S. Thamizhvanan S. Sivakumar S. Santhosh Kumar D. Vinoth Kumar S. Suryakodi K. Balaji T. Rajkumar S. Vimal S. Abdul Majeed G. Taju A. S. Sahul Hameed 《Journal of fish diseases》2019,42(3):447-454
White leg shrimp, Penaeus vannamei, were collected on a monthly basis from grow‐out ponds located at Tamil Nadu and Andhra Pradesh states along the east coast of India for screening of viral and other pathogens. Totally 240 shrimp samples randomly collected from 92 farms were screened for white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV), infectious myonecrosis virus (IMNV) and Enterocytozoon hepatopenaei (EHP). The number of shrimp collected from shrimp farms ranged from 6 to 20 based on the body weight of the shrimp. All the shrimp collected from one farm were pooled together for screening for pathogens by PCR assay. Among the samples screened, 28 samples were WSSV‐positive, one positive for IHHNV and 30 samples positive for EHP. Among the positive samples, four samples were found to be positive for both WSSV and EHP, which indicated that the shrimp had multiple infections with WSSV and EHP. This is the first report on the occurrence of multiple infections caused by WSSV and EHP. Multiplex PCR (m‐PCR) protocol was standardized to detect both pathogens simultaneously in single reaction instead of carrying out separate PCR for both pathogens. Using m‐PCR assay, naturally infected shrimp samples collected from field showed two prominent bands of 615 and 510 bp for WSSV and EHP, respectively. 相似文献
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应用PCR和RT-PCR技术对4种对虾病毒的检测 总被引：5，自引：3，他引：5
探讨了应用PCR和RT－PCR技术对4种主要的对虾病毒进行检测的方法。同时使用该方法检测了对虾天然饵料－－卤虫中的4种对虾病毒。结果显示，含病毒核酸的阳性对照样品分别扩增出了大小为824bp,705bp,260bp和216bp的预期产物，但未能从卤虫样品中检测到此4种病毒的存在。本文报道的病毒检测方法具有快速、灵敏、准确的特点，可以用于进出口贸易中对活体或冰冻对虾，虾苗，对虾饵料等进行相关对虾病毒的检疫，也为制定我国对虾病毒检疫检验规范提供了技术参考。 相似文献