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薰衣草基因转化直接分化受体系统的建立 总被引：1，自引：0，他引：1
以法国孟士德薰衣草(Lavandula angustifolia cv. Munstead)的下胚轴和子叶为研究材料,通过高频再生系统的建立和抗生素敏感性实验,建立了薰衣草基因转化的直接分化受体系统.结果表明:下胚轴的不定芽分化能力比子叶更强,在IAA 0.1 mg·L-1 6-BA 1.0 mg·L-1条件下,10 d苗龄的下胚轴的出愈率和不定芽分化率分别为96.7%和36.7%;所得组培苗在1/2 MS基本培养基上生根率可达95%以上;确定了下胚轴适宜的抗生素筛选浓度,潮霉素的筛选浓度为10 mg·L-1,头孢霉素浓度为200 mg·L-1时即可有效抑制农杆菌的生长,同时又不会对薰衣草不定芽分化产生明显的伤害作用. 相似文献
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以黑果枸杞的叶片和茎段为外植体,采用MS为基本培养基,研究激素对经历愈伤组织诱导、丛生芽诱导以及叶片直接诱导小植株再生途径的影响。实验结果表明,诱导茎段、叶片形成愈伤组织的适宜培养基分别为MS+2,4-D0.3 mg·L~(-1)、MS+2,4-D0.4 mg·L~(-1),其诱导率均为100%;诱导茎段、愈伤组织分化形成丛生芽的适宜培养基分别为MS+6-BA0.2 mg·L~(-1)+KT0.1 mg·L~(-1)、MS+6-BA0.5 mg·L~(-1),而愈伤组织分化出的丛生芽均发生玻璃化现象,其增殖系数分别为32.3倍、47.1倍;诱导叶片分化形成植株的适宜培养基为MS+NAA0.01 mg·L~(-1),其再生植株诱导率为33.3%。结论:黑果枸杞再生能力强,以上途径均能形成再生植株,其最佳的离体繁殖途径为茎段诱导丛生芽形成再生植株。 相似文献
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以樟子松成熟胚为外植体,研究不同的激素组合对樟子松愈伤组织诱导及植株再生的影响。结果表明:诱导愈伤组织的最佳培养基组合为MS+1.5mg/L 2,4-D+0.5mg/L 6-BA;愈伤组织分化的最佳培养基组合为MS+0.2mg/L IAA+1.5mg/L 6-BA;樟子松不定芽伸长生长的适宜基本培养基为1/2MS+0.1%活性炭,诱导生根的最佳培养基组合为1/4MS+0.5mg/L NAA+0.2mg/L IBA。 相似文献
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PEG 6000模拟干旱胁迫下甘蓝型油菜芽期及苗期抗旱指标筛选 总被引：1，自引：0，他引：1
采用聚乙二醇(PEG 6000)模拟干旱胁迫,分析干旱胁迫下8份甘蓝型油菜芽期和苗期抗旱相关指标,鉴定芽期和苗期抗旱性,筛选抗旱评价指标。结果表明,甘蓝型油菜种质芽期和苗期抗旱性强弱不同,芽期抗旱性鉴定的最适PEG 6000浓度为15%,成苗率可作为芽期抗旱性的鉴定指标。通过主成分分析、隶属函数等方法分析苗期相关指标的变化,利用抗旱性综合度量值D值评价甘蓝型油菜苗期抗旱性,结果表明叶片相对含水量(r=0.907~(**))、可溶性蛋白(r=0.921~(**))与抗旱度量值D值呈极显著正相关,丙二醛含量(r=-0.837~(**))与D值呈极显著负相关,这些指标可作为甘蓝型油菜苗期抗旱性的评价指标。 相似文献
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采用10％聚乙二醇(PEG- 6000)模拟干旱胁迫的方法,对41份来源于甘蓝型油菜与蔊菜属间杂交获得的稳定高世代品系和32份来自不同地区的甘蓝型油菜品种进行芽期模拟干旱胁迫处理,以研究干旱胁迫下不同基因型甘蓝型油菜的反应差异.实验结果表明,PEG模拟干旱胁迫后,大部分材料成苗率降低,平均相对成苗率为96.11％,苗高... 相似文献
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以亚洲百合"精粹"(Lilium Asiatic 'Elite')的鳞片、再生形成的叶片和鳞片叶为外植体,进行了不定芽的诱导分化、试管苗小鳞茎诱导、卡那霉素抗性筛选等研究,建立了亚洲百合遗传转化受体系统.结果表明:鳞片诱导最佳分化培养基为MS 6-BA 2.0 mg/L NAA 0.2 mg/L,试管苗小鳞茎诱导培养基为MS IBA 0.5 mg/L 70 g/L蔗糖,试管苗叶片分化培养基为MS TDZ 1.0 mg/L NAA 0.2 mg/L,试管苗鳞片叶分化培养基为MS 6-BA 2.0 mg/L 2,4-D 0.1 mg/L.抗生素敏感性试验表明,百合叶片的卡那霉素选择压为80 mg/L,鳞片叶为115 mg/L. 相似文献
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以唐菖蒲病球的茎尖(0.5毫米)为外植体,接种于MS+BA1ppm+NAA1ppm,3周后部分茎尖分化愈伤组织.将其换接到N6+BA0.5ppm+NAA1ppm+0.4%活性炭培养基上.1周后分化出不定芽,进一步分化出不定根,根系发展 相似文献
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本研究旨在通过根癌农杆菌介导法将ACO基因转入亚洲百合植株中,为植物抗衰老提供理论依据和技术基础。以亚洲百合‘精粹’无菌苗离体小鳞片为外植体,利用农杆菌介导法将携带有抗衰老基因ACO的RNAi载体转化到百合中,观测分析影响百合遗传转化的因素。结果表明:在遗传转化过程中,预培养3 d后,在菌液和共培养基中均添加25 mg/L乙酰丁香酮,将OD600=0.8的根癌农杆菌侵染小鳞片8 min,再共培养3 d,有利于获得最高遗传转化效率,抗性植株经PCR检测部分呈现阳性,初步证明ACO基因已经导入到百合基因组中。 相似文献
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以培养4～5d的紫花苜蓿无菌苗的下胚轴为材料,改良SH(SH大量元素+MS微量元素+MS铁盐+UM有机)+水解酪蛋白(CH)2000 mg/L为基本培养基,对紫花苜蓿愈伤组织诱导和植株再生进行了研究。愈伤组织诱导和继代培养基分别为2,4-D 1.0 mg/L+KT 0.5 mg/L+蔗糖30 g/L及2,4-D 0.05 mg/L+BA0.5 mg/L+蔗糖30 g/L,将获得的愈伤组织转入正交设计的培养基上进行筛选,得到了愈伤组织分化率相对较高的培养基配方:基本培养基+KT 0.4 mg/L+蔗糖20 g/L,其分化率为73.0%。获得的再生芽在生根培养基上(1/2MSO)培养约10d便能获得具有根和叶的再生植株。本试验结果为下一步将来自蓝藻的Na+/H+ an-tiporter基因转入苜蓿,获得转基因耐盐苜蓿奠定了基础。 相似文献
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新疆雪莲体外直接器官发生的快繁研究 总被引：2，自引：0，他引：2
以新疆雪莲无菌苗的下胚轴、子叶、真叶和根为外植体,在MS基本培养基上培养,探索新疆雪莲体外直接器官发生的快繁途径。结果表明:新疆雪莲不同外植体直接再生不定芽的效果不同,以真叶为外植体,直接再生芽频率最高,在MS 6-BA1.0 mg/L(单位下同) NAA0.1 2,4-D0.1上,30 d芽诱导率可达66%;在MS 6-BA0.5 NAA0.05上,芽的增殖生长效果好;在1/2MS NAA0.1上,25 d内生根率达84.7%;在珍珠岩∶腐叶土为1∶3的基质中炼苗,成活率达89.1%。 相似文献
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G.-L. Nan C.-S. Tang A.R. Kuehnle C.I. Kado 《Physiological and Molecular Plant Pathology》1997,51(6):391-399
SuccessfulAgrobacterium-mediated transformation of monocots such as orchids hinges on the induction of virulence(vir)genes in the bacterium, an event that is required for the activation of the T-DNA processing and transfer pathway. In dicots, several plant phenolic compounds, for example acetosyringone, coumaryl alcohol and sinapyl alcohol, are knownvirgene inducers, but in monocots their presence and characteristics are not well established. We report herein the presence and identification of coniferyl alcohol as thevirgene inducer in the monocotDendrobium.At an early stage of development as protocorm-like bodies (PLBs), this orchid contains an aryl β-glycoside, similar to coniferin, whose aglycone moiety is coniferyl alcohol. Coniferyl alcohol is elaborated in high amounts from PLBs and its production or stability is enhanced almost six-fold higher when the PLBs were exposed to light than when maintained in the dark. PLBs produced 11-fold more inducer than leaves. These studies show for the first time that orchids, i.e.Dendrobium,exude coniferyl alcohol as avirgene inducer. 相似文献
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Massimo Zaccardelli Annalisa Spasiano Carlo Bazzi Massimo Merighi 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(1):85-90
A rapid detection method based on PCR amplification of Pseudomonas syringae pv. tomato chromosomal sequences was developed. Primer design was based on the P. syringae DC3000 hrpZPst gene, which maps on a pathogenicity-associated operon of the hrp/hrc pathogenicity island.A 532 bp product corresponding to an internal fragment of hrpZPst was amplified from 50 isolates of P. syringae pv. tomato belonging to a geographically representative collection. The amplification product was also obtained from three coronatine-deficient strains of P. syringae pv. tomato.On the other hand, PCR did not produce any such products from 100 pathogenic and symbiotic bacterial strains of the genera Pseudomonas, Xanthomonas, Erwinia, and Rhizobium and 75 unidentified bacterial saprophytes isolated from tomato plants. The method was tested using leaf and fruit spots from naturally-infected tomato plants and asymptomatic nursery plants and artificially contaminated tomato seeds. The results confirmed the high specificity observed using pure cultures. 相似文献
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H.-J. Lin H.-Y. Cheng C.-H. Chen H.-C. Huang T.-Y. Feng 《Physiological and Molecular Plant Pathology》1997,51(6):367-376
HarpinPssfrom the plant pathogenPseudomonas syringaepv.syringaeis a proteinaceous elicitor that induces a hypersensitive response (HR) in non-host plants. The plant products which recognize harpinPssin the triggering of the HR are not yet known. According to the elicitor-receptor model, we hypothesize that an exogenous cell membrane receptor infiltrated into the intercellular space will interfere with the interaction between harpinPssand the putative receptor. We demonstrate a plant amphipathic protein (AP1) which can postpone the HR induced by harpinPssas well asP. syringaepv.syringae.AP1 was extracted by solubilizing proteins from healthy leaves in the non-polar n-octanol buffer followed by a polar Tris buffer. The amphipathic extracts were then further separated by gel filtration and anion exchange chromatography to obtain highly purified AP1. Similar proteins can be extracted from cotton, tomato, and sweet pepper. The N-terminal amino acid sequence of AP1 is conserved among cotton, tomato, and sweet pepper. The postponement of the harpinPss-mediated HR was characterized as a competitive dosage-dependent pattern of AP1. An analysis of the bacterial population development indicates that the effect of AP1 on the postponement of bacteria-mediated HR was attributed to the suppression of bacterial growth during the early stages of the HR. The time course analysis of the infiltration indicates that the postponement of HR resulted from the co-interaction between AP1 and the bacteria. Based on these results, we suggest that the postponement of bacteria-mediated HR is due to the interference of the interaction between harpinPssand the putative receptor in the plant. Our research provides a new approach to elucidating the role that plants may play in the nonhost response caused by pathogens. 相似文献
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The distributions of two herbicides and two radioactive ions in field plots at two sites were determined at periods up to 187 days following surface applications in the spring. The results demonstrated the variability characteristic of field situations. At one site some fluometuron moved a short distance down the profile but after 187 days most remained above 6 cm whereas at the other site there was essentially no movement below 3 cm. At both sites simazine was almost entirely confined to the top 3 cm. The adsorption characteristics of the two compounds are similar so the greater mobility of fluometuron is probably a consequence of its greater solubility, Measurements of 36Cl? indicated a significant movement of water through the 30 cm depth studied. 144Ce3+ used as a tracer of soil particles was of similar mobility to simazine. In general the movement of chloride and the two herbicides can be interpreted in terms of the concept of mobile and immobile fractions of soil water in which a proportion of the mobile water does not reach equilibrium with solutes in the bulk of the soil. Soil structural effects may therefore be more important than adsorption in controlling the movement of solutes and redistribution in association with soil particles can be significant. A parallel laboratory experiment showed that the results from a standard leaching column procedure did not necessarily indicate field performance. 相似文献
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禾谷孢囊线虫病(cereal cyst nematode,CCN)是由燕麦孢囊线虫Heterodera avenae Wollen.引起的一种重要的小麦病害,在世界上40多个国家均有发生[1].我国于1987年首次发现燕麦孢囊线虫[2],至2012年,已传播到湖北、河南、河北、北京、山东、山西、安徽、江苏、青海、内蒙古、陕西、甘肃、宁夏、天津、西藏、新疆等16个省市自治区[3],危害程度与范围呈逐年加重和扩大的趋势,已对我国小麦生产构成严重威胁.该病造成小麦平均减产20％～40％,严重时高达73％～89％[4].因此,CCN是制约小麦和大麦生产的主要因素之一. 相似文献