共查询到16条相似文献，搜索用时 453 毫秒
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应用组织学方法比较观察了猪繁殖与呼吸综合征病毒(PRRSV)弱毒细胞疫苗与灭活疫苗生殖道黏膜免疫的效果。试验选用5月龄长白×大约克夏二元杂交母猪18头。PRRSV弱毒细胞疫苗和灭活疫苗通过生殖道黏膜免疫接种。结果表明PRRSV弱毒细胞疫苗生殖道黏膜接种组母猪的子宫黏膜上皮内淋巴细胞数较灭活疫苗生殖道黏膜免疫接种组和对照组母猪显著减少(P＜0.01);而且子宫黏膜上皮变矮变薄,变得有些不规则。灭活疫苗生殖道黏膜接种组母猪子宫黏膜上皮内淋巴细胞数与对照组母猪间差异不显著(P＞0.05)。本试验结果显示PRRSV弱毒细胞疫苗经生殖道黏膜免疫接种则对生殖道黏膜上皮内淋巴细胞产生抑制作用。说明PRRSV弱毒细胞疫苗和灭活疫苗经生殖道黏膜免疫效果不理想。 相似文献
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为提高瓶插非洲菊切花的品质,延长瓶插寿命,研究了不同配方保鲜液处理的非洲菊切花的水分平衡、鲜重变化、鲜重变化率和瓶插寿命各指标的变化规律及其关系。结果表明,处理2(蒸馏水+30 g/L蔗糖+200 mg/L 8-HQ+150 mg/L CA+75 mg/L KH2PO4)、处理3(蒸馏水+30 g/L蔗糖+200 mg/L8-HQ+150 mg/L CA+50 mg/L NaHPO4.2H2O)的保鲜剂配方保鲜效果为好,尤以处理2配方的保鲜效果最佳,瓶插寿命是对照(蒸馏水处理)的2.4倍,达到12 d以上。处理2、3非洲菊切花的瓶插寿命与水分平衡值、鲜重变化率分别呈线性和多项式回归关系,二者的水分平衡值与瓶插寿命的相关系数分别为R=0.984 5和R=0.989 5,鲜重变化率与瓶插寿命的相关系数分别为R=0.994 3和R=0.995 5;处理2、3分别在4.351、4.247 d时的水分平衡值为0,在6.633、6.526 d时切花花枝占最初的质量百分数为100%,这些参数为非洲菊切花的瓶插保鲜提供了重要的理论依据。 相似文献
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以大花菊“神马”为材料,利用不同保鲜剂配方对其在保鲜期长短和切花保鲜过程中相关生理指标进行测定,结果表明,2号保鲜剂(去离子水+ 0.03%阿斯匹林+30g/L蔗糖)中切花的花期延长至30天；不同保鲜剂对保鲜过程叶片和花瓣的各项生理指标变化不同,叶片和花瓣的可溶性糖含量较高,可溶性蛋白和SOD变化趋势一致,丙二醛含量趋势不稳定,而叶片和花瓣POD同工酶含量变化趋势相反。研究结果可为菊花切花过程各中保鲜剂的选择提供理论基础,为菊花切花的长短距离运输提供理论依据。 相似文献
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喇叭水仙切花保鲜技术研究 总被引：3，自引：1，他引：2
以Golden Harvest为材料,研究预冷时间和保鲜液配比对喇叭水仙鲜切花保鲜效果的影响,在盛花期取三年生Golden Harvest的新鲜花枝,花枝必须生长一致且接近开放,把鲜切花放在5 ℃、相对湿度70%的冰箱中,分别预冷24、36、48 h,结果表明喇叭水仙鲜切花预冷36 h的花径最先达到最大值,且对花的伤害最轻;以预冷36 h的花枝为材料,对SUG与8-HQC以及AgNO3与6-BA分别进行了不同浓度配比试验,结果表明喇叭水仙的保鲜液的最佳配比为5% SUG＋200 mg/L 8-HQC＋45 mg/L AgNO3＋5 mg/L 6-BA,此保鲜液有利于延长切花瓶插寿命、促进开花度和延缓鲜重的降低速度,此条件下喇叭水仙的瓶插寿命可达14.5天,花开的最大直径可达9.21 cm,维持盛开时间达3.6天,且不易发生霉变腐烂。 相似文献
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几种保鲜剂对百合切花贮后品质的影响 总被引：6，自引：0，他引：6
研究了几种不同的保鲜剂组合对冷藏百合切花贮后品质的影响。结果表明,在瓶插液中加入蔗糖可提高百合切花的观赏品质,50 g/L是最适蔗糖浓度。几种保鲜剂组合中,预处液为2×10-4mol/L硫代硫酸银 + 100g/L蔗糖 + 100mg/L赤霉素, 瓶插液为150 mg/L 8-羟基喹啉硫酸盐 + 50g/L蔗糖 + 20mg/L赤霉素的保鲜剂组合对百合切花的保鲜效果最好。 相似文献
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为探究不同保鲜剂对杜鹃切花采后观赏品质的影响,并筛选出杜鹃切花的适合保鲜剂配方,以东洋杜鹃品种‘红元宝’为研究对象,采用不同保鲜剂对其进行瓶插处理,测定不同处理对切花瓶插寿命、鲜质量变化、水分平衡、质膜稳定性等生理指标和观赏品质的影响。结果表明：100~300 mg/L8-羟基喹啉对‘红元宝’切花的保鲜具有积极作用,可有效延缓花瓣边缘卷曲和萎蔫,促进花径的增大并保持更长的开花时间（18 d）,维持切花体内水分平衡,减缓其鲜质量变化及质膜透性的增加。而0.2~0.6 mmol/L硫代硫酸银处理不利于‘红元宝’杜鹃切花采后品质的保持,反而加剧了切花的衰老。经筛选,确定30 g/L蔗糖+200 mg/L 8-羟基喹啉为‘红元宝’杜鹃切花的最适保鲜剂。 相似文献
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以芍药切花为试材,以蒸馏水为对照(CK1),3％蔗糖+200 mg/L柠檬酸+25 mg/L水杨酸为基本瓶插液(CK2),探索在基本瓶插液中添加200 mg/L羧甲基壳聚糖(CMCS)和20 mg/L纳米银(NS)对芍药切花瓶插品质及采后生理的影响.结果表明:两种药剂均可改善切花水分平衡状态,提升观赏品质.与对照(CK1)相比,CMCS和NS处理芍药切花最大花径分别增加2.17 cm、3.19 cm,瓶插寿命分别延长3.01 d、4.26 d,最佳观赏期分别延长1.87 d、2.75 d.两种杀菌剂对芍药切花生理特性的调控作用相似,均提高了芍药切花花瓣的可溶性蛋白质含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等保护酶活性,降低了相对电导率、丙二醛(MDA)及游离脯氨酸(Pro)含量,其中20 mg/L NS处理的保鲜效果最好. 相似文献
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以月季切花品种Daiana带芽嫩茎为外植体,研究了激素等因素对其无性繁殖系建立的影响。试验结果表明：其诱导分化以B5+BA1.0mg/L(单位下同)+IBA0.25效果较好;继代增殖以B5+BA1.0~2.0+IBA0.25+GA0.3效果好,增殖系数可达5.8~6.2以上;生根以50㎎/LNAA或50mg/LIBA溶液浸泡嫩茎切口基部0.5~1d后,接种于无激素1/2MS基本培养基中效果好,生根率达100%;在基质珍珠岩或蛭石中驯苗,成活率可达94.1%以上。 相似文献
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Effect of pre- and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers 总被引：1，自引：0，他引：1
The naturally short vase-life of cut rose flowers, leads to difficulties with long-distance transportation and subsequent marketing. This study was conducted to determine whether application of salicylic acid (SA) pre- and postharvest could improve vase-life of cut rose flowers of the ‘Black Magic’ variety. Aqueous solutions of 50-200 μM salicylic acid were sprayed on roses grown in a controlled greenhouse about two weeks before harvest and flowers were cut and kept in a vase solution of SA (50-400 μM) to examine SA effects on the vase-life, flower development and senescence, increase/decrease of relative fresh and dry weight, vase solution uptake and activity of an antioxidant enzyme, catalase (CAT). All of these attributes were positively affected by SA treatment. The vase solutions containing SA showed a significant increase in cumulative uptake, relative fresh weight, and CAT activity. Generally, CAT activity in intact and cut flowers decreased during the flower bud development with the lowest activity present at petal senescence. However, SA retarded the decrease of CAT activity during senescence both in flowers and leaves compared to the water control. Postharvest SA application prolonged vase-life in cut rose flowers by improving the reactive oxygen species (ROS) scavenging capacity related to CAT activity and by better regulation of the water balance. 相似文献
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Petal growth associated with flower opening depends on cell expansion caused by water influx. To understand the mechanism of flower opening and to develop a method for improving cut flower quality, we investigated the changes in the amount of soluble carbohydrates and invertase activities in rose (Rosa ‘Meivildo’) petals using attached and cut flowers, and the effects of α-napthylacetic acid (NAA) and methyl jasmonate (MeJA) on cut flowers. Cut rose flowers were harvested at the tight bud stage (TB), or mature bud stage (MB), which is the commercial harvest stage, and 2 days after TB. Cut flowers were immediately treated with deionized water or 1% (w/v) glucose. Fresh weight (FW) and the levels of soluble carbohydrates in petals in cut flowers were lower under postharvest conditions than those of attached flowers during flower opening. Although invertase activities in petals of attached flowers increased drastically during TB and MB, those in the petals harvested at TB did not increase under postharvest conditions. These results suggested that sucrose metabolism, including invertase activity, could be an important factor in cut rose flower opening to a greater extent after harvest. In addition, we tried to control flower opening by affecting invertase activities in petals of cut rose flowers. Cut flowers treated with NAA opened faster and those treated with MeJA opened later than in controls. Levels of soluble carbohydrates and invertase activity in petals were also changed by these treatments. In flowers treated with NAA, activities of both vacuolar and cell wall invertases increased 1 day after treatment and then decreased through to the end of the treatment, although activities in control flowers never increased after harvested. By contrast, cell wall invertase activity in MeJA treated flowers increased 1 day later than with the NAA treatment and remained at a relatively high level until 4 days after treatment compared to the controls. Our results suggest that inducing invertase activity in postharvest conditions may important for the quality of cut roses. 相似文献
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非洲菊切花保鲜中的细菌鉴定和8-HQ抑菌保鲜研究 总被引：1，自引：0，他引：1
非洲菊切花瓶插观赏过程中常出现花茎弯曲和花朵下垂现象，该现象与插花溶液中的细菌滋生有关。本研究分离纯化了非洲菊切花清水瓶插中出现的细菌，通过16S rDNA测序法鉴定出瓶插过程中滋生的细菌主要来自泛菌属、假单胞菌属、寡氧单胞菌属以及克雷伯氏菌属；并研究了8-羟基喹啉（8-HQ）抑制泛菌属细菌增长的最低长效抑菌浓度为150 mg/L；选用8-HQ和蔗糖作为切花保鲜剂主要成分时，其中150 mg/L 8-HQ + 6%蔗糖的组合能最有效延长非洲菊鲜切花开放时间，并保持瓶插溶液清亮程度。 相似文献
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近年来,随着中国古典插花的兴起,牡丹切花保鲜技术取得了一定进展。本文主要从牡丹切花的品种选择、适宜采收期、贮藏和化学保鲜技术的研究进展等方面进行了综述,分析了制约牡丹切花发展的主要问题,提出牡丹切花今后应重点研究的主要方向。 相似文献