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1.
Twenty four male rats (45–65 g) were used to determine the nutritional responses to mixed plant and animal protein diets. Rats were fed various mixtures of sorghum (S), pigeon pea (PP), bread fruit (TAP) plus crayfish (CR) to provide 1.6 g N/100g of daily diet for a 35-day study period. Three different diets were fed to 6 rats assigned to each diet on the basis of body weight. Combination of PP:CR caused increases in N intake and retention, weight gain, and PER higher than for those of the other test groups. Its values for N intake and retention, and BV were higher than for those of the control except for food intake, NPU, weight gain and PER. Substitution of CR (20%) with TAP and PP as supplements to S decreased food intake, weight gain and PER and increased N intake, digested and retained N, NPU and BV. The results appear to indicate that (a) PP:CR blend was better than other blends as judged by the parameters, and (b) TAP and PP were superior to CR alone as supplement to S.  相似文献   

2.
Tryptophan bioavailabilities were estimated in 16 protein sources using 10 day rat growth assays with casein as the reference protein. Growth responses of rats fed test food diets were compared to growth responses of rats fed basal diets with graded levels of tryptophan ranging from 50 to 100 mg of tryptophan/100 g diet. Estimates of tryptophan availabilities were 85–100% for all products except whole wheat cereal (73%) and pinto beans (59%). Results of a previous study on lysine availability indicated that poor response to pinto beans was due either to poor digestibility or to the presence of some unidentified growth inhibitor.  相似文献   

3.
Thirty-six adult Wistar albino male rats (75–128 g) of age 8 weeks were used to study the effect of dehulled and soaked bambara groundnut combined with sorghum or crayfish on iron and phosphorus balances of rats fed for 10 days. Three out of the ten days were for adjustment and the rest for the balance period. Six diets were formulated and fed to the rats. The mixed protein diets provided 10% protein daily for the entire study period. Casein(CA) served as a reference protein. Rats fed the CA diet had significant decreases (p < 0.05) in food intake, maintenance body weight, phosphorus intake and phosphorus balance when compared with those of the test groups. As judged by iron nutriture, dehulled brown bambara groundnut (DBGB) appeared to be superior to the white variety. Soaking seriously affected iron utilization in brown variety. Dehulling produced significant increases (p < 0.05) in phosphorus balance in animals fed the white variety of bambara groundnut compared with the brown variety. On the other hand, soaking produced positive phosphorus balance in rats consuming the brown variety. Addition of crayfish produced a slight nonsignificant decrease (p > 0.05) in rodents eating the brown variety. These results suggested that varietal difference and treatment affected iron and phosphorus utilization in rats fed bambara groundnut in conjunction with sorghum and/or crayfish.  相似文献   

4.
Weanling male rats (45–55 g) were studied to evaluate the protein quality of diets based on combinations of yam (YA); dehulled brown bean (DBB), dehulled white bean (DWB); and corn flour (PA) that provided 1.6 g N/100 g of diet. Casein served as the reference protein. Rats fed combinations of PA, DBB, and DWB had increases in food intake, weight gain, N intake, digested and retained N, and liver N compared to those of the casein control group and the other test groups (P < 0.05). Supplementation of YA with DBB or DWB reduced weight gain, protein efficiency ratio (PER), N intake, digested and retained N, NPU and liver N values of the animals when compared with those fed the casein and the other diets containing YA. Combinations of DBB or DWB with PA appear to be the best mixtures as shown by food and N intakes, digested and retained N, liver N, and weight gain. These mixtures may possibly be economic sources of N for weaning children where these are staples in the diet.  相似文献   

5.
Weanling male rats (45–60 g) were used to compare the supplemental effects to rice protein of bean or crayfish protein. Rats were fed various combinations of rice plus bean (B) or crayfish (C) to provide 1.6 g N/100 g of the daily diet for 28-day growth studies and a 7-day N balance period. Five different diets were fed. Six rats were assigned to each diet on the basis of body weight. Combinations of R:B of 80:20 and of R:C of 80:20 produced food intake, weight gain, PER, N intake, digested and retained N; and liver weight values that were comparable. However, increasing C to 30% of the dietary protein led to reduced value for food intake, weight gain, PER, N intake, digested and retained N, BV, NPU, and liver weight when compared with values obtained when B was increased to 30% (P<0.05). The results appear to indicate that, above a certain level of supplementation, brown bean protein is superior to crayfish as a supplement to rice protein.  相似文献   

6.
Thirty albino weanling male rats (45–55 g) were divided into five groups of six rats each on the basis of body weight and fed combinations of dehulled brown beans (DBB); parboiled rice (R); and cassava meal (G) which provided 10% protein for 35 days. Casein served as the reference protein. Combinations of DBB (50–80%); with R (18–48%); and G (2%) produced varied effects on body weight, nitrogen (N) intake, N digestibility and retention, BV, liver weight and plasma albumin (P<0.05).The DBB:R:G (70:28:2) blend induced decreases in all the parameters tested except for the BV and PER values. The control group produced digested and retained N, protein efficiency ratio (PER), weight gain and liver weight that were significantly higher than for those of the test diets (P<0.05). These results appear to suggest that starchy foods when blended with legumes at low levels produced good quality protein comparable to casein.  相似文献   

7.
Two varieties of mesta (Hibiscus sabdariffa) seeds were analysed for their proximate composition. Their protein (18.8–22.3%), fat (19.1–22.8%) and dietary fiber (39.5–42.6%) contents were found to be high. The seeds were found to be a good source of minerals like phosphorus, magnesium and calcium. Their lysine and tryptophan contents were also high. Sulphur containing amino acids were limiting in this seed protein and the chemical score of mesta seed protein was 40 and 57 for AMV-2 and Bhimili-1 varieties respectively. Mesta seed oil is rich in unsaturated fatty acids (70%), of which linoleic acid constituted 44%. Weanling rats were fed with 10% mesta seed protein before and after cooking for 4 weeks. Food intake of animals receiving raw mesta seed diets was significantly lower than those receiving cooked mesta seed diets as well as the casein control diet. Protein and dry matter digestibilities of raw and cooked mesta seed diets were lower than that of casein control diet. Cooking improved the food intake, gain in body weight, dry matter and protein digestibility of mesta seed diets. PER and NPU of cooked mesta seed diets were significantly higher than the corresponding raw diets. These results indicate that cooked mesta seed protein is of relatively good quality.  相似文献   

8.
A 35-day experimental study consisting of 28-day growth and 7-day calcium (Ca) balance periods was conducted to assess the effects of fibre and N ratios on growth and Ca balance of rats. Growth and Ca balance of rats (45–80 g) fed mixtures of cooked (CS) and uncooked (RS) sorghum and dehulled (DB) and undehulled (UB) bean were studied. The diets contained 10% protein. Casein served as the control protein. The control group ate more food except for the group fed the CS:DU (60:40) diets (P<0.05) had higher values for all parameters tested than the test groups. There were increases in food and Ca intakes, fecal Ca, weight gain, protein efficiency ration (PER), liver weight and composition except for the low moisture value for the CS:DS (60:40) group when N ratios were changed from 80:20 to 60:40. These results appear to indicate that fiber and N ratio had significant effects on growth liver composition and calcium balance of the rats.  相似文献   

9.
The powder forms of soy-based infant formulas obtained from four manufacturers were fed to weanling rats for two weeks, as the sole source of protein in diets containing 8% protein, 20% fat, and adequate amounts of minerals and vitamins. The relative protein efficiency ratio (RPER) and the relative net protein ratio (RNPR) values (casein + methionine=100) of diets containing unsupplemented formulas were 71–81 and 78–85, respectively. Supplementation of the formula diets with lysine (0.2%), methionine (0.2%), threonine (0.1%) or tryptophan (0.05%) increased the level of the supplemental amino acid in rat serum but generally failed to improve the RPER or RNPR values. Addition of all four essential amino acids to the formula diets, however, caused a marked improvement in their protein quality (RPER or RNPR values=100). The data suggested that proteins in soy-based formulas could be marginally co-limiting in several indispensable amino acids.Presented in part at the 202nd annual meeting of the American Chemical Society, New York City, 25–30 August 1991.  相似文献   

10.
A basal diet (containing 20% soybean protein isolate) was supplemented with limiting amino acids (AA, methionine, threonine and tryptophan); wheat bran (WB, 24.3%); amylose (AM, 10%) or a combination of AA and WB or AM to investigate their effects on the levels of blood serum urea nitrogen (BUN), other blood parameters, growth and fecal characteristics in young rats. After 3 weeks feeding, supplementation of the basal diet with AA or WB resulted in improved growth (gain/food ratio) while the addition of AM had no effect on rat growth. Addition of WB to the basal diet had no effect on BUN while the addition of AA and AM caused about 20 and 12% reduction in BUN values, respectively. Rats fed the AA-AM diet had the lowest BUN values (42% lower than those fed the basal diet). Fresh volume, fresh weight, moisture and dry weight of feces in rats fed the WB diets were about four to five fold higher than in those fed the AM diets. The results suggested that BUN may not be a good predictor of growth and protein quality in diets containing AM or a source of dietary fiber (WB).  相似文献   

11.
Four week old male Wistar rats were used to study the effects of scopoletin and cyanide on the histopathology of rat brain. The rats were divided into a control and three experimental groups (2–4) and fed rations containing 0.07 g scopoletin/100 g, 0.07g scopoletin + 1.8 mg cyanide/100 g and 1.8 mg cyanide/100 g, respectively. These levels of scopoletin and cyanide corresponded to levels found in a processed cassava diet. The first group was fed the same ration as the others but without scopoletin and cyanide. The rats were fed these rations for twelve months. Rats from each group were sacrificed at the third, sixth, ninth and twelfth months; the relative brain weight of the rats (% of body weight) and histology of their brains were studied. The lipid peroxide levels of the rat brains were also studied at the twelfth month. The results showed that the relative brain weights of the rats fed scopoletin + cyanide were significantly (p<0.05) less than that of the control from the third month. There were no significant changes in the lipid peroxide levels of the rat brains in the various groups. Histological examination of the brains of the rats sugested that scopoletin is involved in the pathogenesis of the neuropathy seen in cassava consuming populations.  相似文献   

12.
This study investigated the impact of diets containing various levels of avocado (5, 10 and 15%) on gut health in rats fed for six weeks. Avocado-fed rats had significantly higher food intakes while their body weights remained similar to the control diet-fed rats. No significant changes in intestinal bacterial populations (ileum, cecum and colon) were found in rats fed avocado diets compared to the control diet. Ileum and colon tissues of rats fed avocado diets showed significantly higher expression of genes (β-defensin 1, mucin 3 or mucin 4) and a greater number of mucin-producing goblet cells in the colon. The percentage of avocado in the diet had varying effects in altering the biomarkers, whereby diet containing 15% avocado was the more effective diet. This study delivers new knowledge on the role of avocado on gut health in rats.  相似文献   

13.
Effects of coprophagy and coprophagy preventing device on iron bioavailability were evaluated in two experiments. In Experiment 1, rats were fed diets with FeSO4, spinach, bran cereal or cornmeal as the iron source. The rats in each diet group were fitted with collars, sham-collars or not fitted with collars (control). In Experiment 2, rats were fed diets with FeSo4 or green peas as the iron source and were fitted with collars, tail cups, sham-collars or not fitted with any device (control). Preventing coprophagy reduced hemoglobin regeneration efficiency (HRE) of rats fed bran cereal, green peas, spinach or cornmeal diets by 26 (P<.05), 24(P<.05), 22(P<.05) and 11% (not significant), respectively. Preventing coprophagy in rats fed FeSO4 diet did not significantly reduce HRE. Sham-collaring reduced (P<.05) HRE of rats fed the FeSO4 diet by 12 and 13% but did not significantly affect HRE in rats fed food iron sources. It was more convenient and effective to prevent coprophagy with collars than with tail cups. Differences in bioavailability between food and FeSO4 iron due to coprophagy may be explained based on two gastrointestinal nonheme iron pools, complexed and highly soluble.  相似文献   

14.
Protein isolates were extracted from melon, groundnut, and soybean cakes in an aqueous system that could potentially yield both oil and protein from oilseeds quantitatively. Protein isolates obtained were 91.2, 78.6 and 79.5% of the total protein content of melon, groundnut and soybean cakes respectively. Both cakes and isolates were assessed in a rat bioassay while breadfruit, cassava, and corn starch were also investigated as sources of carbohydrate. Results showed that there were no significant differences between feed intake, weight gain and PER of rats fed the cake and the protein isolates except in soybean. Weight gain, PER and NPR were highest in cassava-soybean protein isolate diet followed by cassavacasein diet (control) while performance was only moderate with melon- and groundnutcassava diets. With melon protein isolate, cassava and breadfruit were better carbohydrate sources than corn starch. In simulated weaning diets, cassava-sucrose-melon-soybean protein isolate based diet performed better (weight gain 33.4 g, PER 1.63, NPR 2.23) than a commercial weaning food (weight gain 30.6 g PER 1.57, NPR 2.18) while replacement of sucrose and cassava at 30% by breadfruit yielded a cost effective diet with good performance (weight gain 29.4 g, PER 1.51, NPR 2.12). Diets where soybean protein served as the sole source of protein or breadfruit, white and yellow maize ogi served as the main source of carbohydrate performed only moderately.  相似文献   

15.
We evaluated the effect of supplementation of a wheat bran (WB) diet with fresh carrots (FC) or wet brewer’s grains (WBG) on the growth performance and chemical composition of Tenebrio molitor larvae. Additionally, a digestibility trial was performed to determine the nutritional value of the raw materials used. The control diet was based on WB-only. Two other diets were formulated in which WB was supplemented with FC (FC diet) or with WBG (WBG diet). The experiment was conducted in trays (12 per treatment) and lasted 90 d. Larval weight, feed intake, and excreted feces were controlled in each tray the experiment. The digestibility trial was performed from 48 to 62 d post-hatch. Results showed digestibility coefficients of ashes, crude protein, and gross energy were significantly higher in FC diet compared with the other diets. Consequently, both digestible energy (DE) and digestible protein (DP) contents of FC were also significantly higher than those obtained for WB and WBG diets (on av. +1.12 megajoule [MJ] DE and +9.15 g DP per kg dry matter [DM]; P < 0.003). Mealworms fed FC diet showed significantly higher final weight and average daily gain than those fed the WBG diet (+12.4%), being higher in WBG than in WB diet (+3.5%). Dietary treatment did not affect DM, ashes, ether extract, and crude protein content of mealworms obtained. Some dietary effects on amino acid and fatty acid composition were observed. This study provides novel data and a unique experimental approach to assess the nutritional value of raw materials in mealworms.  相似文献   

16.
The effects of exclusive gari diets prepared using varied fermentation periods on the fasting blood glucose levels (FBGL) and glucose tolerance (GT) of rats were studied. Thirty growing male albino rats divided randomly into 5 groups of 6 rats each were used. The 5 groups were each fed gari diets fermented for 0, 24, 48, 72 hours; and a standard rat diet, respectively, for 8 weeks. Data on the total cyanogen content and % crude protein of the diets; FBGL, GT, body weight, water:feed consumption ratio and clinical observations of the rats were collected. Results showed that the total cyanogen content and % crude protein of the diets were depleted as fermentation periods increased. The FBGL of all the gari-fed rats were significantly elevated and their GT significantly impaired with a significant variation between the groups. The elevated FBGL and impaired GT which were found to increase with increase in fermentation period were strongly inversely correlated to the % crude protein content of the diets (r = –0.92 and –0.96 respectively) suggesting that the diabetogenic potential of gari diets strongly depended on its % crude protein content. Shortened fermentation periods leading to production of gari with high total cyanogen content did not induce higher diabetogenicity.  相似文献   

17.
A feeding trial was conducted in order to evaluate the potential effect on the lipid profile in a experimentally induced situation of hypercholesterolemia of a previously uninvestigated legume (Cicer aretinum L.) widely included in Mediterranean and Latinamerican human diets. Rats fed on a hypercholesterolemic diet containing saturated fat, cholesterol and cholic acid (H) had 123 percent higher serum cholesterol and 62 percent greater triacylglycerols levels than the animals receiving casein (C) protein. The LDL and VLDL cholesterol levels were 1330 percent and 35 percent higher, respectively, and HDL cholesterol 34 percent lower in the group of animals given the H diet as compared to controls. Further feeding of the hypercholesterolemic rats with animal protein (HC) resulted in a significant decrease of triacylglycerols (–70 percent), which reflected the decrease in the VLDL fraction. These effects on the lipid metabolism were more marked when the legumeCicer aretinum L. was present in the diet (HL). Significantly decreased concentrations of total cholesterol (–54 percent) and triacylglycerols (–70 percent) as well as the levels of LDL (–54 percent) and VLDL (–70 percent) were seen in rats fed chickpeas. In conclusion, a differential hypocholesterolemic effect between dietary casein and chickpea intake in a model of hypercholesterolemia induced by the diet was found, with beneficial effects on the lipid metabolism when legume was included in the diet as compared to casein. This suggests, for apparently the first time, that chickpea consumption may have a corrective effect in some alterations of the lipid profile.Abbreviations ANF Antinutritional factors - CD Cardiovascular disease - C Casein diet (Control diet) - H Hypercholesterolemic diet - L Legume diet - SD standard deviation  相似文献   

18.
Bioavailability to rats of iron from fortified grain amaranth   总被引:1,自引:0,他引:1  
In this study, fortified and unfortified grain amaranth seed flour diets and a FeSO4-fortified casein diet (used as a control) were evaluated for their iron (Fe) bioavailability. NaFeEDTA, ferrous fumarate, and FeSO4-fortified grain amaranth were fed to growing Sprague-Dawley weaning male rats. Iron intake, hemoglobin iron (HbFe) gain, Fe availability, total iron binding capacity (TIBC), serum iron, non-haem liver iron and red bloodcell volume (RBV) were determined, and the values were compared with those of the FeSO4-fortified casein diet control. Ferrous fumarate fortified diets gave consistently high values for all these parameters, compared with consistently low values for the amaranth diet without iron fortification.Relative biological values (RBVs) were 0.40, 1.55, 1.75, 1.67 and 1.00 for animals fed on an unfortified amaranth diet, and diets fortified with NaFeEDTA, ferrous fumarate, FeSO4 and casein fortified with FeSO4, respectively. Using FeSO4-fortified casein as control, ferrous fumarate gave a superior RBVs (1.75 vs. 1.00). The RBVs, of the unfortified cereal diets were 40% that of the control, perhaps suggesting low iron absorption from the amaranth cereal. Based on the results of this study, amaranth cereal can be considered an ideal food vehicle for iron fortification. The iron fortification of choice is ferrous fumarate.  相似文献   

19.
Methionine availabilities of 16 test proteins were assessed by comparing ten day rat growth response to the test diets and reference (casein) diets. In a preliminary study, various concentrations of methionine and cystine were fed to determine methionine requirements and effect of excess cystine. Results indicated a methionine requirement of about 550 mg per 100 g diet. Cystine had a sparing affect of 50–55%, i.e., about 300 mg could be used to meet methionine requirements. Further additions of cystine (up to 2.6 times methionine) did not affect rat growth. Methionine availabilities were excellent (88–100%) for 15 of the 16 test foods; only pinto beans (58%) were low, but prior evidence indicates that the poor growth response was due to some factor other than availability.  相似文献   

20.
In a 12-week feeding study, water debittered grains of Andean lupins (Lupinus mutabilis) were tested as unique protein source in growing male and female rats. Before the phsyiological study, the lupin flour was evaluated with regard to its possible content of anti-nutritive substances, such as alkaloids, cyanogenetic glucosides, trypsin inhibitor activity and hemagutanin activity. During the feeding study, parameters such as body weight, food consumption, hemoglobin and blood cells, glucose, urea, total protein, total lipid, cholesterol, uric acid, as well as weight and histopathological examinations of some organs (liver, kidneys, heart, spleen and brain) were controlled. The lupin flour did not show physiologically significant amounts of anti-nutritive substances. Rats fed on lupin diet did not show any statistically different growth performances as compared to the casein fed control animals. Food consumption as well showed no difference between the two diets, although food utilization of casein was slightly better during the first two months. All other observed parameters gave no indication of physiological differences of the performance of the diet. Results of the 12-week feeding test did not give any indication against the use of the water extracted grains of Andean lupins in human and animal nutrition.  相似文献   

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