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木霉菌株T95生物学特性的研究 总被引:5,自引:0,他引:5
木霉菌株T95在PDA培养基、麦芽糖培养基、查彼培养基、苹果树皮煎汁和基本培养基上均能生长。其中在PDA、查彼和基本培养基上,菌丝生长较快,产孢量也较多。在PDA培养基上,菌丝生长的温度范围是15~35℃,产孢的温度范围是10~37℃,菌丝生长和产孢的最适温度为25℃;菌丝生长和产孢的pH值范围是2~11,最适pH值为4。在基本培养基上,葡萄糖为菌丝生长的最佳碳源,木糖为产孢的最佳碳源;蛋白胨和牛肉浸膏是菌丝生长的最佳氮源,天门冬素是产孢的最佳氮源;维生素B1和B2对产孢作用较大。菌丝生长和产孢的最佳微量元素是Mg。分生孢子萌发需要一定的营养,PDA膜好于2%琼脂膜。孢子萌发的温度范围是15~35℃,最适温度为25℃;pH值范围是2~11,pH值为3~5时萌发率较高;接种后8h开始萌发,时间集中在16~24h内。在25℃时,孢子萌发的最适相对湿度为70~80%,最低相对湿度为50%。 相似文献
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《甘肃林业科技》2020,(2)
在温度、营养、酸碱度等不同培养条件下,通过观测羊肚菌M6613菌株的菌丝生长和菌核形成情况,探讨该菌株的生物学特性。试验结果表明:7种复合培养基中梯棱羊肚菌M6613菌株的菌丝均能正常生长且都能形成菌核,最适菌丝生长的复合培养基为PDA综合培养基和PDA土壤培养基,最适菌核形成的复合培养基为PDA麸皮培养基;M6613菌株菌丝生长和菌核形成的最适温度20 ℃,最适酸碱度为pH值6.5;最适菌丝生长和菌核形成的碳源为蔗糖和葡萄糖,尿素对菌丝的生长有抑制作用,(NH_4)_2SO_4和NaNO_4等铵盐存在时,菌丝生长缓慢;最适菌丝生长的氮源为KNO_3,最适菌核形成的氮源为KNO_3和蛋白胨,氮源为(NH_4)_2SO_4和NaNO_3等铵盐时,不形成菌核。 相似文献
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通过试验研究认为,鸡腿蘑最佳母种培养基配方为马铃薯200 g,葡萄糖10g,燕麦粉10g,琼脂20g,水1000ml,最佳出菇培养料配方为棉籽皮90%,燕麦粉8%,蔗糖1%,过磷酸钙0.5%,石膏0.5%,菌丝生长的最适温度为20~25℃,子实体发育最适温度为18~20℃,培养基的最适pH值为6.5~7.5。 相似文献
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桑树轮纹病病原菌的分离鉴定及其生物学特性研究 总被引:1,自引:0,他引:1
2012年6~8月,通过对桑树轮纹病病原菌分离、田间致病性测定、病原菌形态观察,将该病原菌鉴定为桑膝节霉,并对此病病原菌的生物学特性进行了研究。结果表明,不同培养基配方、温度、酸碱度、碳源、氮源、光照对桑树轮纹病病原菌菌丝的生长有一定影响,菌丝生长的最适宜培养基是理查培养基,最适温度是25℃,最适pH值是7.0,最适碳源为葡萄糖,最适氮源为蛋白胨,光照对菌丝生长无显著性差异,菌丝的致死温度和时间分别为85℃和10 min。对该病病原菌生物学特性研究为云南首次报道,对桑树轮纹病的防治具有一定的参考价值。 相似文献
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研究白鳞伞对不同碳源、氮源的利用,及其在不同碳氮比、pH梯度、温度梯度条件下的生长情况和菌丝的致死温度。结果表明,菌丝生长的最适碳源为葡萄糖,最适氮源为牛肉膏,最佳碳氮比为50∶1,在添加VB1的完全培养基中生长较快,最佳pH值为6.5,最适培养温度为20℃,致死温度为50℃,10 min。 相似文献
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通过对桑叶褐斑病病原菌在不同种类培养基培养、不同氮源与碳源、不同温度等6种条件下培养6天后,测定菌落直径,观察菌丝颜色和菌落形态,分析了桑褐斑病病原菌的生物学特性。结果表明,在室内培养条件下,不同的营养成分、 pH值、温度、光照都对菌丝的生长有影响。病原菌菌丝生长的最适宜培养基是燕麦片培养基,适宜生长温度范围是20~30℃,最适生长温度为25℃,光照对菌丝生长无显著影响,最适pH值为9~10,最适碳源为α-乳糖,最适氮源为尿素,菌丝在55℃水浴10 min后则不能生长。 相似文献
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Effect of different nitrogen and carbon sources and their concentrations in liquid media on the mycelial growth of six different ectomycorrhizal (ECM) fungal species was studied. Differences were found in the utilization of the different nitrogen and carbon sources between the fungal species. All the species showed better mycelial growth in the medium containing ammonium as nitrogen source. Growth was low in all species in medium in which nitrogen was supplied in nitrate form. All the ECM isolates investigated showed reduced growth in the medium containing maleic acid as the carbon source. The effect of glucose and di-ammonium hydrogen orthophosphate concentrations on mycelia growth of all the six fungal species was studied with ranges for glucose of 2–40 g/l, and for di-ammonium hydrogen orthophosphate of 2–20 g/l. Cortinarius fulvoconicus and Cortinarius flexipes showed maximal mycelial growth at a glucose concentration greater than 20 g/l. Suillus luteus, Scleroderma citrinum, Laccaria laccata, and Tricholoma aurantium showed maximal growth at a glucose concentration of 20 g/l. All six species showed maximal mycelial growth at 5–10 g/l of di-ammonium hydrogen orthophosphate concentration and with increased concentration mycelial growth in all species decreased. 相似文献
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对分别生长于大兴安岭北坡、南坡的2种灵芝(LZ01、LZ02)的生物学特性进行了研究,通过平板培养方式观测不同温度、pH值、氮源、碳源及不同碳氮比对2种菌种生长速度的影响。结果表明:2种灵芝菌丝适宜生长温度范围为24~28℃,其中LZ01生长于背阴面,最适生长温度在28℃左右;LZ02生长于向阳面,最适生长温度为24℃。2种灵芝在偏酸性环境下生长较好。2种灵芝菌种既能利用有机氮源,也能利用无机氮源,但是有机氮源对其生长较为有利。可溶性淀粉为LZ01适宜生长的碳源,葡萄糖为LZ02适宜生长的碳源,5种碳氮比中比值为40∶1时,菌丝生长速度最快。 相似文献
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K. Rykowski 《Forest Pathology》1976,6(4):211-221
Investigation of nitrogen nutrition in different strains of Armillaria mellea. I. Utilisation of different sources of nitrogen. The assimilation of different forms of nitrogen - NO3-, NH4+ and L-asparagine by isolates of Armillaria mellea from France and Poland was investigated. Both the growth aspect, in which mycelial biomass production was related to the utilisation of nitrogen sources, and the morphological aspect, involving aerial mycelium, sclerotia and rhizomorphs, were studied. Significantly difterent effects were recorded for the influence of nitrogen source, the period of growth and their interaction on the growth of various isolates. Different nitrogen sources significantly affected sclerotial and rhizomorph formation. Isolates of A. mellea, geographically and genetically different, were shown to be also physiologically specialised as was also C. tabescens. 相似文献