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1试验目的根据2021年化肥减量增效项目实施方案要求,2022年将继续开展肥料利用率田间试验,研究新型肥料模式下玉米肥料利用率,现将试验结果总结如下。2试验材料2.1试验时间2021年5月1日至2021年11月1日。2.2试验地点彰武县兴隆山镇花家村,试验地点基本情况(详见表1)。 相似文献
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今年江安县以全国基层农技推广体系改革与建设示范县为契机,紧紧围绕项目建设要求,在全县建立柑桔良种苗木繁育科技试验示范基地、晚棱脐橙标准化科技试验示范基地．新建优质柑桔科技试验示范基地、劣质果园改造科技试验示范基地、柑桔绿色防控科技试验示范基地等5个水果科技试验示范基地。 相似文献
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以星光牌有机沼肥和"碧娇"樱桃番茄为试材,采用不同的施肥方法,研究了沼肥对大棚番茄生长性能的影响。结果表明:沼肥不仅能促进番茄蔓的生长,增强植株的抗逆作用和光合作用,而且还可以提高产量。经移栽后,对照组成活率为90%,试验Ⅰ组为95%,试验Ⅱ组为100%;栽种15d后,对照组、试验Ⅰ组及试验Ⅱ组蔓长平均分别为78、81和85cm,2个试验组蔓的生长速度均比对照组快,试验Ⅱ组比试验Ⅰ组和对照组多长4和7cm,差异不显著(P0.05),30d后,对照组、试验Ⅰ组及试验Ⅱ组蔓长平均分别为128、135和145cm,试验Ⅱ组比试验Ⅰ组和对照组多长10和17cm,差异显著(P0.05);对照组、试验Ⅰ组及试验Ⅱ组的产量分别为1 356、1 645和2 001kg,试验Ⅱ组分别比对照组和试验Ⅰ组多产645kg和356kg,差异显著(P0.05);667m2纯收入,试验Ⅱ组为9 405元,试验Ⅰ组为7 696元,对照组为6 509元,试验Ⅱ组比对照组和试验Ⅰ组分别多收入2 896元和1 709元,效益非常可观。 相似文献
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通过对植物单位面积滞尘量测定试验方法的总结,着重分析试验前植物材料选择、试验操作过程步骤对试验精确度产生的影响。 相似文献
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通豆6号为菜用大豆新品种,该文通过开展品种比较试验、播期试验、栽培密度试验、施肥试验、虫害防治试验等研究,总结了通豆6号菜用大豆在启东地区的高产高效栽培技术,供广大菜农参考。 相似文献
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辣椒病毒病是辣椒生产上重要病害,常造成死棵减产,极大影响了产量与收益。为筛选防治辣椒病毒病效果较好的农药,我们引进了新农药20%迁毒,开展了田间药效试验,现将试验结果介绍如下。1材料与方法1.1试验材料试验药剂为20%迁毒,对照药剂为菌克毒克。1.2试验方法试验设在上海春蔓地蔬菜基地,试验田块为中 相似文献
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AIM: To detect the expression of cytoplasmic inhibitor of apoptosis protein 2 (c-IAP2) and growth arrest-specific gene 1 (GAS1) in Hodgkin's lymphoma (HL) and anaplastic large cell lymphoma (ALCL) and to investigate the role of two genes in the pathogenesis of HL and ALCL.METHODS: HE staining, the antibodies CD30, CD15, CD45RO and CD20 were used to screen the cases of HL and ALCL from 288 cases of lymphoma. The clarified HL and ALCL were subjected for immunohistochemical staining by SP and ABC methods to analyze the expression of c-IAP2 and GAS1. RESULTS: ①The positive rate of c-IAP2 in HL was 25/26(96.1%) while that in ALCL was 6/19(31.6%), there presented statistic significance between HL and ALCL groups(P<0.05), meanwhile the positive rate of GAS1 showed statistic significance between HL and ALCL groups(P<0.05). ②Two cases were showed to be a mixed type combined with large tumor cells of HL and relatively smaller tumor cells of ALCL.CONCLUSION: ①The different expression of c-IAP2 and GAS1 in HL and ALCL implied a different mechanism of oncogenesis and the different defects in the pathway of signal transduction of apoptosis in HL and ALCL;②Few cases showed an overlap and a likely transitional state between HL and ALCL; ③The different expressing manner of GAS1 and c-IAP2 in HL and ALCL implied the potential marks for the differential dignosis of two kinds of lymphoma. 相似文献
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Fruit nutrient content and lipoxygenase (LOX) activity were determined in strawberry fruit to establish a relationship, if it exists, between nutrients, and LOX activity with the fruit malformation and nubbins or button berry disorders. Nearly 17% fruit were affected by malformation and 10% by nubbins in open-field-grown strawberries. ‘Etna’ produced higher proportion of malformed (22.7%) as well as button berries (16.9%) and ‘Sweet Charlie’ the lowest (8.9% and 3.3%, respectively). Dry matter content (%) was lower in malformed (5.2%) and button berries (3.23%) than normal berries (7.41%). The concentration of P and Mg did not differ significantly, but that of N and K was notably higher and of Ca and B was lower in malformed and button berries than normal berries. Consequently, the N/Ca and K/Ca ratios were higher in malformed and button berries. LOX activity was significantly higher in malformed as well as button berries than normal berries, with significant differences among cultivars. The correlations between N, K and malformed and button berries were positive and between Ca and B, and malformed and button berries were negative. Similarly, the correlation between LOX activity and malformed, and button berries were also positive, indicating that excess of N and K, and deficiency of Ca and B are related to the production of malformed and buttons or nubbins in strawberry. 相似文献
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Idowu Babadele Famuwagun Samuel O Agele O.Peter Aiyelari 《International Journal of Fruit Science》2018,18(2):153-176
ABSTRACTThe deployment of plantain shade and irrigation (5- and 10-day) intervals) for enhancing survival, field establishment, flowering and pod production of field-grown cacao seedlings in the dry season was investigated. The effects of dry season irrigation was profound on cacao growth, field establishment (over 90% seedling survival) and development, flowering and pod production. For the non-irrigated moderately shaded cacao, about 30% of the transplanted seedlings survived and the achievable percent survival from the various shade-irrigation combinations ranged from 47–90%. Open sun in combination with 5-day irrigation interval had best results in term of growth (root and shoot development and biomass accumulation) and establishment, flowering and pod production. Lower values of air and soil temperatures were obtained for combination of irrigation and shade regimes (dense and moderate) and higher soil moisture contents. The shade-irrigation combinations ameliorated microclimate and enhanced growth and development, seedling survival, root and shoot development, advanced flowering and promoted uniform fruiting/pod production and total bean yield. The effects of dry season irrigation at 5- or 10-day intervals was profound on cacao growth, field establishment (over 90% seedling survival), flowering and pod production. Irrigated unshaded (open sun) cacao had best results in term of growth (root and shoot development and biomass accumulation) and establishment, flowering and pod production. The achievable percent survival from the various shade-irrigation combinations ranged from 47–90%. The drip irrigation strategy adopted ameliorated dry season terminal drought (hydrothermal stresses) and promoted cacao growth, survival and flower/pod production. 相似文献
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Tardigrade communities are affected by micro and macro-environmental conditions but only micro-environmental variables, and
altitudinal gradients have been studied. We review previous reports of altitudinal effects and evaluate the influence by interacting
macro- (climate, soils, biome, and others) and micro-environmental (vegetation, moss and leaf litter) factors on tardigrade
assemblages at the Sierra de Guadarrama mountain range (Iberian Central System Mountains, Spain). Terrestrial tardigrade assemblages
were sampled using standard cores to collect leaf litter and mosses growing on rocks. General Linear Models were used to examine
relationships between Tardigrada species richness and abundance, and macro- and micro-environmental variables (altitude, habitat
characteristics, local habitat structure and dominant leaf litter type, and two bioclimatic classifications). Variation partitioning
techniques were used to separate the effects of altitude and habitat variation, and to quantify the independent influences
of climate and soil, vegetation structure and dominant type of leaf litter. Altitude shows a unimodal relationship with tardigrade
species richness, although its effect independent of habitat variation is negligible. The best predictors for species richness
were bioclimatic classifications. Separate and combined effects of macro-environmental gradients (soil and climate), vegetation
structure and leaf litter type are important determinants of richness. A model including both macro- and micro-environmental
variables explained nearly 60% of tardigrade species richness in micro-scale plots. Abundance was significantly related only
to soil composition and leaf litter type. Tardigrade abundance was not explained by macro-environmental gradients analysed
here, despite a significant correlation between abundance and richness. 相似文献
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AIM: To investigate the effects of Chinese medicine, Jinan injection, on ultrastructure and mitochondria in cultured lung cancer cell lines. METHODS: The cultured lung cancer cell lines PG and PAa were used and divided into 4 groups: control (C), cisplatin (DDP), Jinan (JA) and Jinan in combination with cisplatin (DJ), respectively. The changes of morphology and mitochondria membrane potential, intracellular Ca2+ and pH in every group were observed by inverted microscope and electronic microscope as well as by using flow cytometry, staining by rhodamine, Fluo-3 and BCECF, respectively. RESULTS: Degeneration cells showed chromatin condensation and peripheral congregation. In cytoplasm autophage lysosome increased and myelinoid body was seen easily. In mitochondria structure, where the space between the inner and outer membranes of these organelles expanded as the matrix was compressed. The electron-dense or swelled was observed as vacuole degeneration and its matrix showed electron-lucent. Compared to control, mitochondria membrane potential increased in every group after 24 h and 48 h treatment. DDP increased intracellular calcium ion in PG cells, however, in PAa cells, JA and DJ decreased it. Intracellular pH got lower at 24 h and higher at 48 h in PG and PAa cells. There were significance in every group vs control in PG and PAa by statistic t-test (P＜0.01). CONCLUSION: Apoptosis was induced in PG and PAa cell lines by Jinan injection and DJ. Mitochondria matrix displayed electron-dense, mitochondrial potential, intracellular calcium ion and pH showed an increasing trend. Mitochondria damages may play an important role in apoptosis induced by Jinan and DJ. 相似文献
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In a test with ‘Bartlett’ and ‘d'Anjou’ pear on 8 Pyrus (P.) rootstocks, leaves of ‘Bartlett’ were high in S and Fe on ‘Bartlett’ seedlings (BS), in Zn on P. ussuriensis Maxim. seedlings (USS), and in B on seedlings of P. calleryana Decne (Cal). Leaves of ‘d'Anjou’ were high in P, Zn and B on BS, in N on USS, and in S on Cal. Both ‘Bartlett’ and ‘d'Anjou’ were high in K and Ca on P. betulaefolia Bunge seedlings (Bet). Fruit of both ‘Bartlett’ and ‘d'Anjou’ were high in N, K and Mg on Bet and Cal and high in Ca on Cal and USS. With both ‘Bartlett’ and ‘d'Anjou’, moderately high correlations were found between leaf and fruit concentrations of K and Mg; positive correlations were high for Ca and S with ‘Bartlett’ and for Zn and B with ‘d'Anjou’. There was a very high negative correlation between leaf and fruit Ca with ‘d'Anjou’. 相似文献
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AIM：To establish and evaluate a hyperbilirubinemia and kernicterus model in neonatal SD rats. METHODS：Three-day-old SD rats were randomly divided to 7 experimental groups by litter and body weight, and were intraperitoneally injected with physiological saline (control group), and 6.25 μg/g (T1), 12.5 μg/g (T2), 25 μg/g (T3), 50 μg/g (T4), 100 μg/g (T5) and 200 μg/g (T6) bilirubin, respectively, twice every day for 3 d. All rats were photographed, weighed and killed 12 h after the last injection. The contents of the stomach were drawn and weighed, and the index was calculated. The liver/body weight ratio was determined, the total and unconjugated bilirubin in the serum and total bilirubin in the brain were calculated, and the contents of ATP and water in the brain were measured. HE and Nissl staining were used to observe the pathological changes. RESULTS：Along with the increase in bilirubin, gradual exacerbation of the general performance of the rats, and yellowish discoloration of the skin and mucous membranes were observed.The degree of the activity gradually reduced, and the weight gain was suppressed. The weight of T6 group showed negative growth, and the 72 h mortality rate was close to 100%. The mortality rate in T4 and T5 groups continued to rise 1 week after injection. Compared with control group, the weight of stomach contents and stomach content index in T3~T5 groups significantly decreased (P<0.01). The liver/body weight ratio in T5 group was significantly higher (P<0.05). The concentrations of serum total and unconjugated bilirubin and brain bilirubin levels in T1~T5 groups were gradually increased, while the brain water content had no difference among groups. The brain ATP content in T1~T5 groups increased at the beginning and reached its peak in T3 group, but compared with control group, that in T4 group and T5 group significantly reduced (P<0.05). HE results showed that, with the increase in bilirubin concentration, the number of the neurons in the cerebral cortex of the rats decreased. In T4 group and T5 group, the neuronal structural disorder, cell swelling, nuclear pyknosis, fragmentation and dissolution, increase in non-homogeneous structure of the material dyed red, and disappearance of nuclear staining were observed. Nissl staining showed that, compared with control group, in T1 group and T2 group, the cortical neurons became smaller, Nissl bodies decreased, and cytoplasmic staining changed little. The cortical neuronal tigroid body color became light gradually, neuron cells become small, and Nissl bodies decreased obviously in T3, T4 and T5 groups. The T4 and T5 rat ce-rebral cortical neurons dissolved or even disappeared. CONCLUSION：Newborn 3-day-old SD rats receiving intraperitoneal injection of bilirubin at doses of 12.5, 25, 50 and 100 μg/g, 2 times a day, can induce hyperbilirubinemia, and 50 and 100 μg/g can cause bilirubin encephalopathy. 相似文献
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HU Zhi-cheng ZHU Jia-yuan ZHU Bin GUO Dong CHEN Bin ZHANG Kai HU Kun-hua LI Ming-tao TANG Bing 《园艺学报》2011,27(9):1802-1806
AIM: To investigate and screen the sensitive proteins in the formation mechanism of pathological scars by comparing the results of differential proteomic analysis between pathological scars and normal skin.METHODS: Two-dimensional gel electrophoresis was used to detect the protein expression profiles in 8 keloid patients, 8 hypertrophic scar patients and 3 matched normal skin patients.The proteins that showed differential expression of over 4-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.RESULTS: A two-dimensional protein profiling comparison between pathological scars and normal skin was successfully established.On average, 2 978 spots in keloid, 2 975 spots in hypertrophic scar and 3 053 spots in normal skin were identified using gel analysis software.Compared with normal skin, there were totally 36 differentially-expressed proteins in keloid and hypertrophic scar identified from the spots of over 4-fold change, including 16 proteins in both keloid and hypertrophic scar (8 up-regulated and 8 down-regulated), 11 only in keloid (9 up-regulated and 2 down-regulated) and 9 only in hypertrophic scar (4 up-regulated and 5 down-regulated).CONCLUSION: Proteomic analysis can identify the proteins with variance of pathological scars versus normal skin, thus providing probable new clues to reveal the formation mechanism of pathological scars. 相似文献