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《Scientia Horticulturae》2006,108(1):79-85
The classifications of oriental cymbidiums that are native to Korea, China, Taiwan and Japan were examined by molecular analysis. A total of 21 cymbidiums, 15 species including three Cymbidium gyokuchin, 4 Cymbidium kanran and 2 Cymbidium goeringii cultivars, were analyzed by using randomly amplified polymorphic DNA (RAPD) to determine the interspecific and intraspecific relationships. Twenty-two primers were used in the RAPD analysis to distinguish, by comparing differences in DNA banding patterns, all species and cultivars. Similarity values ranged from 0.501 for Cymbidium aloifoilum and C. kanran to 0.935 for Cymbidium ensifolium and Cymbidium marginatum with analysis of total bands score. Phylogenetic tree analysis of the RAPD results from the 21 cymbidiums identified specific groupings. The cymbidiums could be divided into two clusters based upon ecological traits. One trait was temperate zone preference, with each cymbidium preferring either an Asian or subtropical temperate zone. The group that comprised the subtropical cymbidiums was C. aloifoilum, Cymbidium insigne and Cymbidium lowianum. Additionally, we found that Cymbidium lancifolium and Cymbidium aspidistrifolium could be separated based on different flowering physiology and unique leaf form. The groups identified by morphological, physiological and ecological characteristics were in full agreement with those determined by RAPD analysis. The phylogenetic tree derived from the RAPD results was similar to that of the traditional classification. The data acquired from this study could be used for identification and classification of other orchid genera and oriental cymbidium. 相似文献
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兰花新品种‘曙光’是以西藏虎头兰‘黄素花’（Cymbidium tracyanum L. Castle）为母本和大雪兰（Cymbidium mastersii Griff. ex Lindl.）为父本杂交选育而成。植株四季常绿,叶片带状,14 ~ 20片,花葶高51 ~ 56 cm,着花6 ~ 12朵。花朵自然水平展开9.5 ~ 13.0 cm,有香味。萼片乳黄色至淡绿色（渐变）,花瓣稍宽（1.6 cm ± 0.2 cm）,淡黄绿色,具有淡红色纵脉,唇瓣左右两裂片之间有两行金黄色毛,合蕊柱腹面为淡红色。花期40 ~ 60 d,瓶插期20 ~ 45 d。适宜在亚热带或气候相近的温暖地区保护地栽培。 相似文献
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‘霞光’是由母本西藏虎头兰‘黄素花’（Cymbidium tracyanum L. Castle）和父本大雪兰
（Cymbidium mastersii）杂交选育而成的兰花新品种。四季常绿，叶带状，14 ~ 20 枚；花葶高52 ~ 55 cm，
着花6 ~ 12 朵，花朵自然水平展开9.6 ~ 13.2 cm，有香味。萼片和花瓣均为金黄至卵黄色，切花瓣稍宽
（1.4 ± 0.2）cm，唇瓣左右裂片之间有两行金黄色毛，合蕊柱背和腹面为红色。花期40 ~ 60 d，瓶插期
20 ~ 45 d。适宜在亚热带或气候相近的温暖地区保护地栽培。 相似文献