共查询到19条相似文献，搜索用时 234 毫秒
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重金属污染是指由于人类对矿山过度开采、金属冶炼与加工及其它化工操作,造成的铅(Pb)、汞(Hg)、镉(Cd)、铬(Cr)等重金属以各种形式进入生态环境中,在其中留存、积累和迁移而造成的生态污染,土壤重金属污染的治理已成为目前环保关注的焦点。植物在长期进化过程中形成了根系富集、细胞壁束缚、跨膜转运等多种应对重金属胁迫的应答机制。microRNA(miRNA)是一类调控基因表达的重要小分子RNA,通过调控靶基因的表达而参与植物的生长发育、胁迫应答等生理代谢活动,它们同样在植物应答重金属胁迫过程中发挥重要作用。该研究对土壤中重金属污染现状进行了综述,并阐述了重金属胁迫条件下相关miRNA表达调控情况,以期为更好地研究植物miRNA应答重金属胁迫的调控机制提供参考依据。 相似文献
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植物中miRNA响应非生物胁迫研究进展 总被引：1，自引：0，他引：1
MicroRNA(miRNA)是一类长度为20~24nt的非编码RNA,在植物遭受非生物胁迫时,通过介导靶基因mRNA发挥着非常重要的作用。本文就低温、外源ABA和干旱等非生物胁迫对miRNA的调控机制进行综述,进一步阐明miRNA响应非生物胁迫的机理。 相似文献
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脱落酸与植物非生物逆境抗性研究进展 总被引：1，自引：0，他引：1
作为一种非常重要的植物激素,脱落酸(ABA)在应对环境胁迫和调控植物对非生物胁迫耐受性方面发挥了重要的作用。该文综述了ABA的生物合成和代谢途径、信号传导途径以及ABA在调控植物应对干旱胁迫、非干旱胁迫、盐胁迫等非生物胁迫中的作用;并对ABA分子合成机制和ABA在调控植物产生应对非生物胁迫抗性的分子机制等方面进行了展望。 相似文献
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microRNA（miRNA）是一类包含20～30个核苷酸的非编码RNA分子,在植物的生长发育和适应外界各种环境胁迫的过程中起着非常重要的作用。植物中miRNA的数量和种类都很多,而高通量测序技术的出现大大加快了miRNA的发现过程。本文综述了近年来利用高通量测序技术进行的逆境下植物体内miRNA的研究状况,以反映当前植物miRNA的发现、功能和进化等方面的最新研究进展。 相似文献
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《园艺学报》2020,(5)
microRNA(miRNA)是在转录后水平调控基因表达的一类非编码的小分子RNA。植物miRNA最早于2002年在拟南芥中发现,柑橘miRNA的研究始于2005年,现已发现了许多保守的miRNA和新的miRNA。对参与柑橘生长发育及对生物学胁迫和非生物学胁迫应答过程的miRNA进行了归纳,并对cis-miR156、cis-miR396和cis-miR3954在柑橘中的功能研究进行了总结。与模式植物和大田作物相比,柑橘miRNA的功能研究相对滞后,新发现miRNA的功能有待深入研究。最后对利用柑橘特异的病毒载体和基因定点编辑技术加快柑橘miRNA功能研究进行了展望。 相似文献
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植物在低温胁迫下的过氧化氢代谢及信号转导 总被引：3，自引：1，他引：2
过氧化氢(Hydrogen peroxide, H2O2 ) 是植物细胞代谢过程中产生的一种活性氧, 被认为对细胞具有毒害作用。然而越来越多的研究证明, H2O2是植物细胞抵抗胁迫反应过程中的重要信号分子, 尤其H2O2在植物受到低温胁迫时所具有的信号调控功能越来越受到关注。作者综述了低温胁迫下的H2O2代谢情况, 并从H2O2与乙烯、水杨酸等信号分子的互作效应, 以及H2O2对抗冷基因、根系吸水性的调控来探讨其在抗冷信号转导过程中的功能及作用机制。 相似文献
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Circular RNAs (circRNAs) are a type of covalently closed circular non-coding RNAs that are expressed across varieties of life forms. CircRNAs have a variety of characteristics such as stable structures, tissue-specific expression and the function of microRNA sponges in the regulation of genes. CircRNAs are abundant, highly expressed in neural tissues, emerging evidence also indicates that circRNAs play an important role in the pathophysiologic process of Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and other neurological diseases. As one of the most important neurological diseases, the pathogenesis of brain damage and repair after ischemia has not yet been evaluated, and stroke related circRNAs are of great significance. In this review, we summarize related regulations, and the potential regulatory mechanisms of cicrcRNAs in cerebrovascular diseases, and the important role of circRNAs in post stroke pathophysiologic process is also discussed. CircRNAs are expected to be a new drug target, and provide new theoretical basis for the diagnosis and treatment of cerebrovascular diseases. 相似文献
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Although urban habitats contribute to the conservation of species diversity, urbanization has significantly reduced biodiversity by causing environmental changes such as habitat loss and fragmentation. Therefore, research on urban biodiversity studies has become increasingly important. Historical heritage sites are recognized as important habitats in remnant green spaces in urban areas. We aimed to evaluate the role of historical sites in conserving biodiversity in urban areas. As the land in these historical sites is not modified, they have the potential to conserve biodiversity through continued maintenance activities such as mowing and tree cutting. In Japan, Tamagawa-josui, a waterway that runs from west to east through the Tokyo megacity (35° 40′ N, 139° 25′ E), has been recognized as a civil engineering heritage landmark that preserves water utilization technology from the early modern period (1600s, Edo-era). The present study examined the relationships between plant diversity and green space in a historic site of a megacity (i.e., Tamagawa-josui) and determined the factors that influence plant diversity. Specifically, we examined the relationships between plant species indices (species richness and species compositions) and environmental factors (management, environmental conditions, and landscape factors). The present study analyzed spatial changes in the plant species composition in Tamagawa-josui. We demonstrated that tree canopy openness was positively correlated with plant species richness, and the increased disturbance associated with developing historical sites as urban parks was negatively correlated with native plant species richness. In addition, there was significant species turnover in the plant community from upstream to downstream in Tamagawa-josui, which could largely be explained by spatial factors. We demonstrated that historical sites can provide potential habitats for the conservation of the plant species diversity, which is based on the effectiveness of the management of their vegetation. 相似文献
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鸟巢菌是一类重要的高等担子菌,具有重要的经济及应用价值,因其具有药用价值及其代谢产物能够抑制植物病害。近年来,国内外对其形态、生物学特性、生理、代谢产物、发酵工程、生物防治等方面开展了一些研究,目前,随着科学技术的不断发展,对鸟巢菌更加深入的研究却严重滞后,例如分子生物学的研究等,有待进一步加强且存在较大的发展空间。该研究通过国内外文献分析,综述了鸟巢菌相关方面的研究,在此基础上认为应从鸟巢菌生防菌筛选及开发、分泌蛋白功能以及关键代谢酶功能等方面开展深入研究,以期为进一步利用该菌奠定坚实的基础。 相似文献
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不同品种蓝莓植株失绿的叶片和土壤诊断及分析 总被引：1，自引：0，他引：1
以4种蓝莓失绿植株的根际土和黄化叶片为试材,分析了各根际土壤的K、P、Ca、Mg、Zn、Cu、有效铁含量和植株叶面积、叶绿素a、叶绿素b、有效铁含量等指标,以探究蓝莓植株黄化的原因。结果表明:4种蓝莓的各级黄化叶的叶面积(重度黄化叶下降15.5%～37.5%)、叶绿素b(重度黄化叶下降43.8%～71.0%)和有效铁含量低于健康叶(重度黄化叶下降14.7～19.7%),重度黄化叶的叶绿素a含量低于健康叶(下降72.5%～76.8%);4种蓝莓黄化植株的根际土壤有效铁含量低于健康植株(重度黄化植株的下降16.7%～22.1%),依次为健康植株>轻度黄化>中度黄化>重度黄化;重度黄化植株根际土壤的K、P含量高于或等于健康植株;除"蓝金"品种外,其它品种重度黄化植株根际土壤的Ca含量低于或等于健康植株;除"伯克利"品种外,其它品种根际土壤的Mg含量与健康植株相等;除"莱克西"品种外,其它品种根际土壤的Zn含量低于健康植株;4种蓝莓重度黄化植株根际土壤的Cu含量与健康植株相等。因此,蓝莓植株叶片黄化的原因是由于土壤中的有效铁含量较低,K、P含量过高,从而造成叶片有效铁含量过低,叶绿素含量下降,最终导致叶片黄化。 相似文献
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番茄S-腺苷蛋氨酸脱羧酶基因SlSAMDC1的克隆与序列分析 总被引：1，自引：0，他引：1
以蔓陀罗S-腺苷蛋氨酸脱羧酶全长cDNA序列为信息探针, 筛选NCBI番茄EST数据库, 依据同源EST信息,经人工拼接、RT-PCR及RACE技术验证,获得了1个新的SAMDC基因家族成员,命名为SlSAMDC1(GenBank登录号：EF550528),并利用染色体步移技术克隆了879 bp的上游调控区域。SlSAMDC1 cDNA序列全长1 847 bp, 5′-UTR和3′-UTR分别长523 bp、241 bp;存在3个ORF(微型uORF、小型uORF和主ORF),主ORF编码360个氨基酸的SAMDC酶原;SlSAMDC1基因组序列全长3 648 bp,有3个内含子,均位于5′-UTR,所有内含子的剪切位点均符合真核生物“GT-AG”规则。SlSAMDC1基因与其它植物来源SAMDC基因同源性较高,与人、大肠杆菌以及酵母的同源性较低。表达谱分析发现,SlSAMDC1基因在番茄根、茎、叶、花蕾、果实等器官中均表达,果实中的表达量相对较高。生物信息学分析表明,SlSMDC1基因的上游调控序列存在多个顺式作用元件,如W-box、TATA-box、CAAT-box等。 相似文献
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ZHU Wen-si TANG Chun-mei ZHU Jie-ning LIN Qiu-xiong FU Yong-heng DENG Chun-yu YANG Hui RAO Fang WU Shu-lin SHAN Zhi-xin 《园艺学报》2016,32(8):1351-1356
AIM: To determine circular RNA (circRNA) profiles in the diabetic mouse myocardium, and to investigate the effect of circRNA_000203 on fibrotic phenotypes in cardiac fibroblasts.METHODS: Masson trichrome staining was performed on the myocardium of the diabetic db/db mice and the non diabetic db/m control mice. circRNA expression profile in the diabetic myocardium was detected by circRNAs microarray. The expression of circRNA_000203 was determined by real time fluorescence quantitative PCR (RT-qPCR). Recombinant circRNA_000203 adenovirus was prepared for enforced the expression of circRNA_000203 in mouse cardiac fibroblasts. The expression of Col1a2, Col3a1and α-SMA was determined in circRNA_000203-modified cardiac fibroblasts, respectively. RESULTS: Masson trichrome staining showed that fibrosis was increased in the diabetic mouse myocardium. The results of circRNA array detection revealed that circRNAs were dysregulated in the diabetic myocardium. circRNA_000203 was up-regulated in the diabetic myocardium. Significant over-expression of circRNA_000203 was achieved in the cardiac fibroblasts after infection with the recombinant circRNA_000203 adenovirus. The mRNA and protein expression of Col1a2, Col3a1 and α-SMA was significantly increased in the cardiac fibroblasts with over-expression of circRNA_000203.CONCLUSION: circRNA_000203 is up-regulated in the diabetic mouse myocardium. It has pro-fibrotic effect on the cardiac fibroblasts. 相似文献