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1.
Tropical legume cover crops are important components in cropping systems because of their role in improving soil quality. Information is limited on the influence of nitrogen (N) fertilization on growth of tropical legume cover crops grown on Oxisols. A greenhouse experiment was conducted to evaluate the influence of N fertilization with or without rhizobial inoculation on growth and shoot efficiency index of 10 important tropical cover crops. Nitrogen treatment were (i) 0 mg N kg?1 (control or N0), (ii) 0 mg N kg?1 + inoculation with Bradyrhizobial strains (N1), (iii) 100 mg N kg?1 + inoculation with Bradyrhizobial strains (N2), and (iv) 200 mg N kg?1 of soil (N3). The N?×?cover crops interactions were significant for shoot dry weight, root dry weight, maximal root length, and specific root length, indicating that cover crop performance varied with varying N rates and inoculation treatments. Shoot dry weight is considered an important growth trait in cover crops and, overall, maximal shoot dry weight was produced at 100 mg N kg?1 + inoculation treatment. Based on shoot dry-weight efficiency index, cover crops were classified as efficient, moderately efficient, and inefficient in N-use efficiency. Overall, the efficient cover crops were lablab, gray velvet bean, jack bean, and black velvet bean and inefficient cover crops were pueraria, calopo, crotalaria, smooth crotalaria, and showy crotalaria. Pigeonpea was classified as moderately efficient in producing shoot dry weight.  相似文献   

2.
Little information is available about the effects of cover crops on soil labile organic carbon (C), especially in Australia. In this study, two cover crop species, i.e., wheat and Saia oat, were broadcast-seeded in May 2009 and then crop biomass was crimp-rolled onto the soil surface at anthesis in October 2009 in southeastern Australia. Soil and crop residue samples were taken in December 2009 to investigate the short-term effects of cover crops on soil pH, moisture, NH4+–N, NO3−–N, soluble organic C and nitrogen (N), total organic C and N, and C mineralization in comparison with a nil-crop control (CK). The soil is a Chromic Luvisol according to the FAO classification with 48.4 ± 2.2% sand, 19.5 ± 2.1% silt, and 32.1 ± 2.1% clay. An exponential model fitting was employed to assess soil potentially labile organic C (C 0) and easily decomposable organic C for all treatments based on 46-day incubations. The results showed that crop residue biomass significantly decreased over the course of 2-month decomposition. The cover crop treatments had significantly higher soil pH, soluble organic C and N, cumulative CO2–C, C 0, and easily decomposable organic C, but significantly lower NO3−–N than the CK. However, no significant differences were found in soil moisture, NH4+–N, and total organic C and N contents among the treatments. Our results indicated that the short-term cover crops increased soil labile organic C pools, which might have implications for local agricultural ecosystem managements in this region.  相似文献   

3.
Indicators of soil quality, such as microbial biomass C and N (MBC, MBN) and enzyme activities (EAs), involved in C, P, N, and S cycling, as affected by dryland cropping systems under conventional (ct) and no tillage (nt) practices were evaluated for 5?years. The soil is sandy loam with an average of 16.4% clay, 67.6% sand, and 0.65?g kg?1 OM at 0?C10?cm. The crops evaluated were rotations of grain sorghum (Sorghum bicolor L.) or forage sorghum (also called haygrazer), cotton (Gossypium hirsutum), and winter rye (Secale cereale): grain sorghum?Ccotton (Srg?CCt), cotton?Cwinter rye?Csorghum (Ct?CRye?CSrg), and forage sorghum?Cwinter rye (Srf?CRye). The tillage treatments did not affect soil MB and EAs of C cycling (i.e., ??-glucosidase, ??-glucosaminidase, ??-galactosidase), P cycling (alkaline phosphatase, phosphodiesterase), and S cycling (arylsulfatase)??except for separation due to tillage for Srf?CRye and Ct?CRye?CSrg observed in PCA plots when all EAs were evaluated together. After 3?years, rotations with a winter cover crop history (Ct?CRye?CSrg and Srf?CRye) enhanced soil MBN (up to 63%) and EAs (21-37%) compared to Srg?CCt. After 5?years, Srg?CCt and Ct?CRye?CSrg showed similar soil MBC, MBN, EAs, total carbon (TC), and organic carbon (OC). A comparison of Srg?CCt plots with nearby continuous cotton (Ct?CCt) research plots in the same soil revealed that it took 5?years to detect higher TC (12%), MBC (38%), and EAs (32?C36%, depending on the enzyme) under Srg?CCt. The significant improvements in MB and EAs found, as affected by dryland cropping systems with a history of winter cover crops and/or higher biomass return crops than cotton, can represent changes in soil OM, nutrient cycling, and C sequestration for sandy soils in the semiarid Texas High Plains region. It is significant that these soil changes occurred despite summer crop failure (2003 and 2006) and lack of winter cover crops (2006) due to lack of precipitation in certain years.  相似文献   

4.
Dissolved organic nitrogen (DON) is increasingly recognized as a pivotal pool in the soil nitrogen (N) cycle. Numerous devices and sampling procedures have been used to estimate its size, varying from in situ collection of soil solution to extraction of dried soil with salt solutions. Extractable organic N (EON) not only consists of DON but contains also compounds released from soil biomass and desorbed organic matter. There is no consensus whether DON or EON primarily regulates N mineralisation in soil, and their contribution to N mineralisation has not been quantified simultaneously. We evaluated three sampling procedures on their ability to determine the dynamic of dissolved organic N pools. The three procedures were the determination of DON in 1) soil solution collected by centrifugation, and the determination of EON in 2) a 0.01 M CaCl2 extract of field moist or 3) dried soil. We added unlabeled leek and 15N-labeled ryegrass residues to a loamy sandy soil to create a temporarily increase in DON and EON, to stimulate microbial activity, and to test whether the source and dynamics of the three pools differ. We also tested whether the flow of N through DON or EON was associated with the production of inorganic N using 15N isotope tracing. Sampling procedures significantly affected the amount, but not the dynamics and origin of the three organic N pools. DON and EON (determined on field-moist and dried soils) showed all a significant increase upon crop amendment and returned to their background concentrations within 10 to 30 days. The fraction of DON and EON originating from the crop residue slightly decreased over 138 days and was not different for DON and EON. Field moist extraction of a loamy sandy soil with 0.01 M CaCl2 gave a reliable estimate of the concentration of in situ dissolved organic N. In contrast, extraction of dried soil significantly increased EON compared to DON. The agreement in dynamics, 15N enrichment and C-to-N ratio’s indicate that dissolved and extracted organic N have a similar role in N mineralisation. Our results also suggest that they make a minor contribution to N mineralisation; changes in the turnover rate of EON were not associated with changes in the net N mineralisation rate.  相似文献   

5.
The root is an important organ which supplies water and nutrients to growing plants. Data related to root growth and nutrient uptake by tropical legume cover crops are limited. The objective of this study was to evaluate root growth of tropical legume cover crops and nutrient uptake and use efficiency under different phosphorus (P) levels. The P levels used were 0 (low), 100 (medium) and 200 (high) mg kg?1 of soil and 5 cover crops were evaluated. Root dry weight, maximum root length, specific root length were significantly influenced by P and cover crop treatments. Maximum values of these root growth parameters were achieved with the addition of 100 mg P kg?1 soil. The P X cover crops interaction for all the macro and micronutrients, except manganese (Mn) was significant, indicating variation in uptake pattern of these nutrients by cover crops with the variation in P rates. Overall, uptake pattern of macronutrients was in the order of nitrogen>calcium>potassium>magnesium>phosphorus (N > Ca > K > Mg > P) and micronutrient uptake pattern was in the order of iron>manganese>zinc>copper (Fe > Mn > Zn > Cu). Cover crops which produced maximum root dry weight also accumulated higher amount of nutrients, including N compared to cover crops which produced lower root dry weight. Higher uptake of N compared to other nutrients by cover crops indicated that use of cover crops in the cropping systems can reduce loss of nitrate (NO3?) from soil-plant systems. Increase in root length and root dry weight with the addition of P can improve nutrient uptake from the soil and less loss of macro and micronutrients from the soil-plant systems.  相似文献   

6.
Roots are important organs that supply water and nutrients to growing plants. Data related to root growth and nutrient uptake by tropical legume cover crops are limited. The objective of this study was to evaluate root growth of tropical legume cover crops and nutrient uptake and use efficiency under different phosphorus (P) levels. The P levels used were 0 (low), 100 (medium), and 200 (high) mg kg?1 of soil, and five cover crops were evaluated. Root dry weight, maximum root length, and specific root length were significantly influenced by P and cover crop treatments. Maximum values of these root growth parameters were achieved with the addition of 100 mg P kg?1 soil. The P?×?cover crops interactions for all the macro- and micronutrients, except manganese (Mn), were significant, indicating variation in uptake pattern of these nutrients by cover crops with the variation in P rates. Overall, uptake pattern of macronutrients was in the order of nitrogen (N) > calcium (Ca) > potassium (K) > magnesium (Mg) > P and micronutrient uptake pattern was in the order of iron (Fe) > Mn > zinc (Zn) > copper (Cu). Cover crops which produced maximum root dry weight also accumulated greater amount of nutrients, including N, compared to cover crops, which produced lower root dry weight. Greater uptake of N compared to other nutrients by cover crops indicated that use of cover crops in the cropping systems could reduce loss of nitrate (NO3 ?) from soil–plant systems. Increase in root length and root dry weight with the addition of P can improve nutrient uptake from the soil and lessen loss of macro- and micronutrients from the soil–plant systems.  相似文献   

7.

Purpose

Soil acidification is universal in soybean-growing fields. The aim of our research was to evaluate the effects of soil additives (N fertilizers and biochar) on crop performance and soil quality with specific emphasis on ameliorating soil acidity.

Materials and methods

Four nitrogen treatments were applied as follows: no nitrogen (N0), urea (N1), potassium nitrate (N2), and ammonium sulfate (N3), each providing 30 kg N ha?1. Half plot area of the N1, N2, and N3 treatments was also treated with biochar (19.5 t ha?1) to form N-biochar treatments (N1C, N2C, N3C). Both bulk and rhizosphere soils were sampled separately for the following analyses: pH, exchangeable base cations (EBC), exchangeable acidity (EA), total inorganic N (IN), total N (TN), and microbial phospholipid fatty acids (PLFAs). Soybean biomass and nutrient contents were also determined. Correlation analysis was applied to analyze the relationships between soil chemical properties and soybean plant parameters.

Results and discussion

With N-biochar additions (N1C, N2C, N3C), soil chemical properties changed as follows: pH increased by 0.6–1.2 units, EBC, IN, and TN increased by 175–419, 38.5–54.7, and 136–452 mg kg?1, respectively, and PLFAs increased by 23.6–40.9 nmol g?1 compared to the N0 in the rhizosphere. Microbial PLFAs had positive correlations with soil pH; EBC; exchangeable K, Ca, Na, and Mg; TN; IN; NH4 +; and NO3 ? (r?=?0.66–0.84, p?<?0.01). There were negative correlations between PLFAs and EA or exchangeable Al (r?=??0.64, ?0.66, p?<?0.01), which indicated that the additives increased microbial biomass by providing a suitable environment with less acid stress and more nutrients. The additives increased soil NH4 + and NO3 ? by promoting soil organic N mineralization and reducing NH4 + and NO3 ? leaching. Moreover, the soybean seed biomass and the nutrient contents in seeds increased with N-biochar additions, especially in the N3C treatment.

Conclusions

N-biochar additions were effective in ameliorating soil acidity, which improved the microenvironment for more microbial survival. N-biochars influenced N transformations at the plant–soil interface by increasing organic N mineralization, reducing N leaching, and promoting N uptake by soybeans. The soil additive ammonium and biochar (N3C) were best in promoting soybean growth.
  相似文献   

8.

Purpose

Sulfur (S) plays a vital role in plant metabolism, and the detrimental impact of S deficiency in several field crops has increased over the last 30?years. The bio-availability of organic S to plant depends on arylsulfatase (ARS), a key enzyme for S mineralization in soil. In this study, we characterized the spatial variability of ARS activity in an agricultural soil cropped with the rape plant (Brassica napus). Because rape requires relatively large amounts of S per yield unit compared to most grain crops, it is very sensitive to S deprivation similarly to the other plants of the Brassicaceae family, with consequences for seed quality and yield.

Materials and methods

The spatial variability of (a) ARS activity, (b) the abundance of culturable bacteria possessing the ARS, and (c) soil properties (temperature, soil pH, SO 4 2? -S (sulfate-S) content, labile carbon (C) and nitrogen (N), soil microbial biomass carbon SMB-C, and nitrogen SMB-N) was estimated at 40 sites within a rape field, using a 4?×?5-m sampling grid. Geostatistics were used to model the spatial distribution of the measured variables, and relationships between variables were tested using linear statistical analyses.

Results and discussion

The total ARS activity showed a low variability ranging between 69.0 and 153.1???g?p-nitrophenol?g?1?dry?soil?h?1 while the abundance of the culturable ARS community ranged within one order of magnitude. The distribution of both the abundance and activity of the ARS community exhibited spatial dependence in 800?m2 agricultural field.

Conclusions

The spatial pattern of ARS activity in the field was correlated with several soil properties, and results suggest that soil pH, labile C and N, and SBM-C/SBM-N ratio were the main parameters linked to the ARS activity rather than the abundance of the culturable ARS bacterial community or the SO 4 2? -S concentration.  相似文献   

9.

Purpose

The aim of this study was to understand the effect of nitrogen fertilization on soil respiration and native soil organic carbon (SOC) decomposition and to identify the key factor affecting soil respiration in a cultivated black soil.

Materials and methods

A field experiment was conducted at the Harbin State Key Agroecological Experimental Station, China. The study consisted of four treatments: unplanted and N-unfertilized soil (U0), unplanted soil treated with 225?kg?N?ha?1 (UN), maize planted and N-unfertilized soil (P0), and planted soil fertilized with 225?kg?N?ha?1 (PN). Soil CO2 and N2O fluxes were measured using the static closed chamber method.

Results and discussion

Cumulative CO2 emissions during the maize growing season with the U0, UN, P0, and PN treatments were 1.29, 1.04, 2.30 and 2.27?Mg?C?ha?1, respectively, indicating that N fertilization significantly reduced the decomposition of native SOC. However, no marked effect on soil respiration in planted soil was observed because the increase of rhizosphere respiration caused by N addition was counteracted by the reduction of native SOC decomposition. Soil CO2 fluxes were significantly affected by soil temperature but not by soil moisture. The temperature sensitivity (Q 10) of soil respiration was 2.16?C2.47 for unplanted soil but increased to 3.16?C3.44 in planted soil. N addition reduced the Q 10 of native SOC decomposition possibly due to low labile organic C but increased the Q 10 of soil respiration due to the stimulation of maize growth. The estimated annual CO2 emission in N-fertilized soil was 1.28?Mg?C?ha?1 and was replenished by the residual stubble, roots, and exudates. In contrast, the lost C (1.53?Mg?C?ha?1) in N-unfertilized soil was not completely supplemented by maize residues, resulting in a reduction of SOC. Although N fertilization significantly increased N2O emissions, the global warming potential of N2O and CO2 emissions in N-fertilized soil was significantly lower than in N-unfertilized soil.

Conclusions

The stimulatory or inhibitory effect of N fertilization on soil respiration and basal respiration may depend on labile organic C concentration in soil. The inhibitory effect of N fertilization on native SOC decomposition was mainly associated with low labile organic C in tested black soil. N application could reduce the global warming potential of CO2 and N2O emissions in black soil.  相似文献   

10.
A method for calculating the relative uptake (R) of added N and indigenous soil N by a legume (Trifolium subterraneum) and non-legume (Lolium rigidum), growing together, was investigated in two pot experiments. In the first experiment, 15N-labelled sodium nitrate was applied to the soil surface at rates equivalent to 0.3 or 1.0kg N ha?1. Twenty one days later, the legume had fixed about 95% of its total N and this was unaffected by N addition. There was no difference in R values between legume and non-legume at both N rates.In the second experiment using a soil of higher total N, sodium nitrate or ammonium sulphate were surface-applied at a rate equivalent to 1 kg N ha?1 and harvests were made at 3, 6, 12 and 27 days after N addition. Fixation of atmospheric N2 by the legume did not begin until day 12 but accounted for about 40% of the total N assimilated by the legume by day 27. There was no difference in R values between legume and non-legume throughout the growth period when sodium nitrate was applied. However, when ammonium sulphate was added to label to soil N, the uptake of added N relative to indigenous soil N was greater for the non-legume than the legume. This caused an overestimation (51 vs 43%) of the proportion on N fixed by the legume when compared with that for the control or sodium nitrate treatments.  相似文献   

11.
Changes in the soil microbial communities and networks were monitored after planting the cover crop for 9 years. The field experiment included plots with a cover crop and without a cover crop but with weed control, and two subplots with or without chemical fertilizer (192 kg N ha?1, 108 kg P2O5 ha?1, and 168 kg K2O ha?1 each year). After applying the cover crop and chemical fertilizer for 9 years, the composition and activity of bacterial and fungal communities changed significantly (p?<?0.05), with the cover crop had greater effects than the chemical fertilizer on the composition of the soil microbial community. The relative abundances of 22 selected genera (in Firmicutes and Bacteroidetes) and two selected classes (Ascomycota) related to cover crop residue degradation increased significantly in the presence of the cover crop (p?<?0.05). Network analysis showed that the cover crop decreased the number of positive links between bacterial and fungal taxa by 25.33%, and increased the negative links by 22.89%. The positive links among bacterial taxa increased by 16.63% with the cover crop, mainly among Proteobacteria (increase of 39), Firmicutes (16), Actinobacteria (five), and Bacteroidetes (10). The links among fungal taxa were less than among bacterial taxa and were not significantly affected by cover crop. Taxa such as Thaumarchaeota, unidentified_Nitrospiraceae, unidentified_Nitrosomonadaceae, Faecalibacterium, Coprococcus_3, and Ruminococcaceae_NK4A214_group dominated the network without the cover crop but they were not dominant with the cover crop. The relative abundances of potential genes involved with the degradation of cellulose, hemicellulose, and cello-oligosaccharides increased significantly with the cover crop. Therefore, the SOC and TN contents were enhanced by the cover crop with the increase of the soil enzyme activities. Thus, the apple yield was improved by the cover crop.  相似文献   

12.

Background

A high use-efficiency of fertilizer N remains essential to sustain high crop productivity with low environmental impact. However, little is known on the long-term lability of mineral fertilizer N.

Aims

To quantify crop uptake and leaching of 15N-labelled mineral fertilizer that has been retained in an agricultural soil for 25–30 years in crops with variable growing season.

Methods

A field plot received 15N-labelled mineral fertilizers over a period of 5 years and was then kept under arable cropping for 12 years. After relocation to 16 lysimeters, the topsoil grew set-aside grassland for the next 13 years. Then crop uptakes and leaching losses of 15N remaining in soil was tested over a 2-year period by either converting set-aside grass to production grassland, or by replacing it with spring barley (+/− autumn cover crop) or vegetation-free fallow. All treatments received unlabelled mineral N fertilizers.

Results

Crop uptake and leaching of 15N were generally highest in the first test year after termination of the set-aside. The leaching of residual 15N in soil declined in the order: vegetation-free soil (4.7%), spring barley (1.9%), spring barley + cover crop (0.7%) and production grassland (0.2%). Corresponding losses for the second leaching period were 2.7%, 0.9%, 0.4% and 0.06%. There was a fixed relationship between leaching losses of 15N and total N.

Conclusions

After residing in soil for 25–30 years, the lability of labelled mineral N fertilizer residues appeared slightly higher than the lability of bulk soil N. Autumn vegetation was crucial for reducing leaching losses.  相似文献   

13.
The mineralization and availability of cover crop N to the succeeding crop are critical components in the management of soil N to reduce N leaching. The effects of several leguminous and non-leguminous cover crops on soil N availability, N mineralization potential, and corn (Zea mays L.) yield were examined. The cover crops had variable effects on soil N availability and corn yield and N uptake. Because of the rapid mineralization of the cover crops following incorporation, the inorganic N levels in the soil sampled in mid-May 1992 (4 weeks after incorporation of cover crops), rather than the potentially mineralizable N, rate constants, initial potential mineralization rate, or cumulative N mineralized over 14 weeks, correlated well with N concentrations, C:N ratios, or the N added in the cover crops. However, the inclusion of potentially mineralizable N with inorganic N in a multiple regression improved the variability in the corn yield and the N uptake accounted for. Since extensive mineralization had occurred before the 21 May sampling, the potentially mineralizable N was affected more by the soil organic N and C than by the N concentrations of the cover crops. The presidedress NO3 --N test levels were well predicted by the inorganic and potentially mineralizable N (R 2=0.89, P<0.01), although the test levels were better in predicting corn yield and N uptake. If the available soil N test needs to be made earlier than recommended by the presidedress NO3 --N test, both inorganic and potentially mineralizable N are needed to better predict the corn yield and N uptake in the soils.  相似文献   

14.

Purpose

Crop straws and animal manure have the potential to ameliorate acidic soils, but their effectiveness and the mechanisms involved are not fully understood. The aim of this study was to evaluate the effectiveness of two crop (maize and soybean) straws, swine manure, and their application rates on acidity changes in acidic red soils (Ferralic Cambisol) differing in initial pH.

Materials and methods

Two red soils were collected after 21 years of the (1) no fertilization history (CK soil, pH 5.46) and (2) receiving annual chemical nitrogen (N) fertilization (N soil, pH 4.18). The soils were incubated for 105 days at 25 °C after amending the crop straws or manure at 0, 5, 10, 20, and 40 g kg?1 (w/w), and examined for changes in pH, exchangeable acidity, N mineralization, and speciation in 2 M KCl extract as ammonium (NH4+) and nitrate plus nitrite (NO3??+?NO2?).

Results and discussion

All three organic materials significantly decreased soil acidity (dominated by aluminum) as the application rate increased. Soybean straw was as effective (sometimes more effective) as swine manure in raising pH in both soils. Soybean straw and swine manure both significantly reduced exchangeable acidity at amendment rate as low as 10 g kg?1 in the highly acidic N soil, but swine manure was more effective in reducing the total acidity especially exchangeable aluminum (e.g., in the N soil from initial 5.79 to 0.50 cmol(+) kg?1 compared to 2.82 and 4.19 cmol(+) kg?1 by soybean straw and maize straw, respectively). Maize straw was less effective than soybean straw in affecting soil pH and the acidity. The exchangeable aluminum decreased at a rate of 4.48 cmol(+) kg?1 per pH unit increase for both straws compared to 6.25 cmol(+) kg?1 per pH unit from the manure. The NO3??+?NO2? concentration in soil increased significantly for swine manure amendment, but decreased markedly for straw treatments. The high C/N ratio in the straws led to N immobilization and pH increase.

Conclusions

While swine manure continues to be effective for ameliorating soil acidity, crop straw amendment has also shown a good potential to ameliorate the acidity of the red soil. Thus, after harvest, straws should preferably not be removed from the field, but mixed with the soil to decelerate acidification. The long-term effect of straw return on soil acidity management warrants further determination under field conditions.
  相似文献   

15.
ABSTRACT

Broccoli (Brassica oleraceaL. var. italica) and lettuce (Latuca sativaL.) were grown under greenhouse conditions with nitrogen (N) from a cover crop mixture of rye (Secale cerealeL.) and crimson clover (Trifolium incarnatumL.) and ammonium nitrate (NH4NO3). Individual cover crop species were produced with non-enriched or enriched (5 atom % NH4 15NO3) Hoagland Nutrient Solutions resulting in enriched rye [0.799% atom % 15N, 24:1 carbon (C):N ratio] and enriched clover (0.686% atom % 15N, 19:1 C:N ratio). Cover crops were applied as an equal mixture of rye and clover at 1884, 3768, and 5652 kg·ha? 1 dry weight to supply 26, 52, and 78 kg·ha? 1 N. Enriched materials were only applied at the 3768 kg·ha? 1 rate, either as enriched rye plus non-enriched clover or non-enriched rye plus enriched clover. Additional treatments consisted of an unfertilized control and three NH4NO3 fertilizer rates; 112, 224, and 336 kg·ha? 1 N for broccoli and 70, 140, and 210 kg·ha? 1 N for lettuce. Combination treatments were the standard cover crop rate (3768 kg·ha? 1) plus the lowest N fertilizer rate for each vegetable. Cover crops did not increase yield of either broccoli or lettuce, and contributed only 17% of the N in broccoli and 15% of the N in lettuce. The majority of cover crop 15N remained in the soil: 54.8% and 81.3% of rye and clover N, respectively, after broccoli harvest; and 68.1% and 79.2% of rye and clover N, respectively, after lettuce harvest. Broccoli plant tissue recoveries were 8.0% of the rye and 11.0 % of the clover 15N; while lettuce plant tissue recoveries were 6.3% (rye) and 4.1% (clover). Broccoli yield could not be assessed due to lack of floret development, but dry matter accumulation was maximized at 224 kg·ha? 1N. Lettuce yield and fertilizer N recovery efficiency (by mass balance) was maximized at 140 g·ha? 1 N.  相似文献   

16.

Purpose

Evergreen broad-leaved forest ecosystems are common in east China, where they are both ecologically and economically important. However, nitrogen (N) addition over many years has had a detrimental effect on these ecosystems. The objective of this research was to evaluate the effect of 4 years of N addition on microbial communities in an evergreen broad-leaved forest in southern Anhui, China.

Materials and methods

Allochthonous N in the form of aqueous NH4NO3 and phosphorus (P) in the form of Ca(H2PO4)2·H2O were applied at three doses with a control (CK, stream water only without fertilizer): low-N (50 kg N ha?1 year?1), high-N (100 kg N ha?1 year?1) and high-N+P (100 kg N ha?1 year?1 + 50 kg P ha?1 year?1). Quantitative PCR analysis of microbial community size and Illumina platform-based sequencing analysis of the V3-V4 16S rRNA gene region were performed to characterize soil bacterial community abundance, structure, and diversity.

Results and discussion

Bacterial diversity was increased in low-N and high-N treatments and decreased in the high-N+P treatment, but α-diversity indices were not significantly affected by N additions. Proteobacteria, Acidobacteria, and Actinobacteria were the predominant phyla in all treatments, and the relative abundance of different genera varied among treatments. Only soil pH (P = 0.051) showed a weak correlation with the bacterial community in CK and low-N treatment.

Conclusions

The composition of the bacterial community and the abundance of different phyla were significantly altered by N addition. The results of the present study indicate that soil bacterial communities in subtropical evergreen broad-leaved forest are, to a certain extent, resilient to changes derived from N additions.
  相似文献   

17.
ABSTRACT

In order to understand how soil microbial biomass was influenced by incorporated residues of summer cover crops and by water regimes, soil microbial biomass carbon (C) and nitrogen (N) were investigated in tomato field plots in which three leguminous and a non-leguminous cover crop had been grown and incorporated into the soil. The cover crops were sunn hemp (Crotalaria juncea L., cv ‘Tropic Sun’), cowpea (Vigna unguiculata L. Walp, cv ‘Iron clay’), velvetbean (Mucuna deeringiana (Bort) Merr.), and sorghum sudangrass (Sorghum bicolor × S. bicolor var. sudanense (Piper) Stapf) vs. a fallow (bare soil). The tomato crop was irrigated at four different rates, i.e., irrigation initiated only when the water tension had reached ?5, ?10, ?20, or ?30 kPa, respectively. The results showed that sorghum sudangrass, cowpea, sunn hemp, and velvetbean increased microbial biomass C by 68.9%, 89.8%, 116.8%, and 137.7%, and microbial N by 58.3%, 100.0%, 297.3%, and 261.3%, respectively. A legume cover crop, cowpea, had no statistically significant greater effect on soil microbial C and N than the non-legume cover crop, sorghum sudangrass. The tropical legumes, velvetbean and sunn hemp, increased the microbial biomass N markedly. However, the various irrigation rates did not cause significant changes in either microbial N or microbial C. Soil microbial biomass was strongly related to the N concentration and/or the inverse of the C:N ratio of the cover crops and in the soil. Tomato plant biomass and tomato fruit yields correlated well with the level of soil microbial N and inversely with the soil C:N ratio. These results suggest that cover crops increase soil microbiological biomass through the decomposition of organic C. Legumes are more effective than non-legumes, because they contain larger quantities of N and lower C:N ratios than non-legumes.  相似文献   

18.

Purpose

We investigated the effects of weed control and fertilization at early establishment on foliar stable carbon (δ13C) and nitrogen (N) isotope (δ15N) compositions, foliar N concentration, tree growth and biomass, relative weed cover and other physiological traits in a 2-year old F1 hybrid (Pinus elliottii var. elliottii (Engelm) × Pinus caribaea var. hondurensis (Barr. ex Golf.)) plantation grown on a yellow earth in southeast Queensland of subtropical Australia.

Materials and methods

Treatments included routine weed control, luxury weed control, intermediate weed control, mechanical weed control, nil weed control, and routine and luxury fertilization in a randomised complete block design. Initial soil nutrition and soil fertility parameters included (hot water extractable organic carbon (C) and total nitrogen (N), total C and N, C/N ratio, labile N pools (nitrate (NO3 ?) and ammonium (NH4 +)), extractable potassium (K+)), soil δ15N and δ13C. Relative weed cover, foliar N concentrations, tree growth rate and physiological parameters including photosynthesis, stomatal conductance, photosynthetic nitrogen use efficiency, foliar δ15N and foliar δ13C were also measured at early establishment.

Results and discussion

Foliar N concentration at 1.25 years was significantly different amongst the weed control treatments and was negatively correlated to the relative weed cover at 1.1 years. Foliar N concentration was also positively correlated to foliar δ15N and foliar δ13C, tree height, height growth rates and tree biomass. Foliar δ15N was negatively correlated to the relative weed cover at 0.8 and 1.1 years. The physiological measurements indicated that luxury fertilization and increasing weed competition on these soils decreased leaf xylem pressure potential (Ψxpp) when compared to the other treatments.

Conclusions

These results indicate how increasing N resources and weed competition have implications for tree N and water use at establishment in F1 hybrid plantations of southeast Queensland, Australia. These results suggest the desirability of weed control, in the inter-planting row, in the first year to maximise site N and water resources available for seedling growth. It also showed the need to avoid over-fertilisation, which interfered with the balance between available N and water on these soils.  相似文献   

19.
We conducted a microcosm experiment with soil being sterilized, reinoculated with native microbial community and subsequently manipulated the bacterivorous nematodes, including three treatments: without (CK) or with introducing one species of the two bacterivores characterized with different body size but similar c-p (colonizer-persister) value (Rhabditis intermedia and Protorhabditis oxyuroides, accounted for 6 and 59% of bacterivores in initially undisturbed soil, respectively). We monitored the N2O and CO2 emissions, soil properties, and especially quantified gross N transformation rates using 15N tracing technique after the 50 days incubation. No significant differences were observed on soil NH4 + and NO3 ? concentrations between the CK and two bacterivores, but this was not the case for gross N transformation rates. In comparison to CK, R. intermedia did not affect soil N transformation rates, while P. oxyuroides significantly increased the rates of mineralization of organic N to NH4 +, oxidation of NH4 + to NO3 ?, immobilization of NO3 ? to organic N and dissimilatory NO3 ? reduction to NH4 +. Furthermore, the mean residence time of NH4 + and NO3 ? pool was greatly lowered by P. oxyuroides, suggesting it stimulated soil N turnover. Such stimulatory effect was unrelated to the changes in abundance of bacteria and ammonia-oxidizing bacteria (AOB). In contrast to CK, only P. oxyuroides significantly promoted soil N2O and CO2 emissions. Noticeably, bacterivores increased the mineralization of recalcitrant organic N but decreased soil δ13C-TOC and δ15N-TN values, in particular for P. oxyuroides. Combining trait-based approach and isotope-based analysis showed high potential in moving forward to a mechanistic understanding of bacterivore-mediated N cycling.  相似文献   

20.
To what extent might a crop with increased plant N uptake efficiency and/or N demand increase plant biomass and soil carbon storage, decrease N leaching, and reduce the need for N fertilisation? This was assessed for a fertilised sandy loam site in central Sweden cultivated with spring barley for a four year period using a process based crop and soil simulation model (SOILN) calibrated to fit observations of field experiments with non-modified crops. Crop properties were changed in accordance with previous model applications to other crops with higher N uptake and utilisation efficiencies, to resemble potential effects of breeding. For the modified crops a doubling of daily uptake efficiency of soil mineral N and/or increase of radiation use efficiency by 30%, increased plant biomass by 3%–30%, decreased N leaching by 1%–30% and increased soil organic carbon (SOC) content by 1–12?g?C?m?2 year?1. The larger changes were mainly due to increased uptake efficiency. Fertilisation of the modified spring barley crop could be reduced while still producing the same plant biomass as the non-modified crop. The plant biomass to N leaching ratio of the modified crops increased. The simulated changes in plant biomass and SOC were sensitive to weather conditions suggesting that in situ experiments would need to cover a large range of weather conditions to evaluate the performance of new crop traits under climatic variability. The study suggests a strong need that field experiments are accompanied with model applications, when exploring the potential of the modified crops under variable conditions.  相似文献   

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