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1.
Bacterial blight(BB), which is caused by Xanthomonas oryzae pv. oryzae(Xoo), is an important rice disease responsible for significant yield losses. In the rice-growing regions of South China where BB outbreaks are common, the resistance of cultivars with BB resistance genes Xa4 and Xa21 has been lost because of rapid changes in the Xoo population structure and virulence. In this study, 421 diverse rice accessions were evaluated regarding their resistance to two Xoo strains, namely GD1358(C5) and IV, which are prevalent pathotypes in South China and overcame the resistance of Xa4 and Xa21, respectively. Using the 4.8 mio filtered SNP dataset, we conducted a genome-wide association study, which identified 13 loci associated with BB resistance, including eight new quantitative trait loci(QTL) and five QTL harboring known BB resistance genes: Xa3/Xa26, xa5, Xa35(t), Xa36(t), Xa40, Xa43(t), and xa44(t). Intriguingly, a steep peak was detected on chromosomes 5 and 11. Six QTL including three new ones, were distributed on chromosome 11, whereas a new QTL q BB5.1 and a known QTL were detected on chromosome 5. Haplotype analyses indicated that the LOC;s05 g01610(Os PRAF2) gene within the q BB5.1 region, which encodes a PRAF protein, is associated with BB resistance. Furthermore, Os PRAF2 knockout lines generated using the CRISPR-Cpf1 system were significantly more resistant to Xoo strains than the wild-type plants. Our results provide researchers and breeders with useful information regarding QTL and gene resources,which may be relevant for developing new BB-resistant rice cultivars.  相似文献   

2.
Hirschmanniella or yzae is one of the most im-portant pathogenic nematodes of rice. Thereare few data on the Integrated Pest Manage-ment (IPM) of H. oryzae except for somestudies on the chemical control of this nema-tode. A study on the IPM of H. oryzae wascarried out in Guangzhou from 1991 to 1995.This paper presented the results on the control  相似文献   

3.
Isolates of X. oryzae pv. or yzae (X. o. o. ) resistant to Saikuzuo [N, N-methylene-di (2 amino-5-sulfurhydrogen-1, 3, 4-thioazole)]were selected from laboratory and field for the study of their characteristics.  相似文献   

4.
Saikuzuo (N, N'-methylene-di (2-amino-5-sulfurhydrogen-1, 3, 4-thiodiazole)) a good bactericideagainst rice bacterial blight disease, has been usedin China for more than 20 years. In this study,the sensitivity of X. oryzae pv. oryzae (Xoo) tosaikuzuo was tested in vitro and in vivo. Seventy-seven and 11 isolates were collected from Hexian,Anhui Province, and Liuhe, Jiangsu Province, re-  相似文献   

5.
Hirschmanniella oryzae is one of the most important pathogenic nematodes of rice. There are few data on the Integrated Pest Management (IPM) of H.  相似文献   

6.
Isolates of X.oryzae pv.oryzae (X.o.o.) resistant to Saikuzuo [N, N-methylene-di(2-amino-5-sulfurhydrogen-1, 3, 4-thioazole)] were selected from laboratory and field for the study of their characteristics. The laboratory resistant mutants had no pathogenicity on rice, and the field resistant isolates did not show resistance to Saikuzuo in vitro. These strains showed cross resistance to homologue bactericide Dikuzuo (TH-128), but seems no resis  相似文献   

7.
From 1986 to 1993, a set of near-isogenic japonica rice lines with three major genes Xa-3, Xa-4, and Xa-12 for resistance to bacterial blight(Xanthomonas oryzae pv.oryzae) were developed and designated as CBB3, CBB4, and CBB12 respective-  相似文献   

8.
We studied the relationship between the resistance to Xanthomonas oryzae pv, oryzae (XOO) and active oxygen species (AOS).Materials used were Yushuinuo (high resistant) and Zhefu 802 (high susceptible).Paraquat (PQ) specially generated superoxide anion (O2 ) and Tiron (4, 5-dihydroxy-1.3-benzenedisufonic acid).  相似文献   

9.
Pyricularia oryzae is a fungal pathogen of rice. Few researches had been reported on the analysis of its genome, partly because the genomes can‘t be separated by general agarose gel electrophoresis.We herein report a technique to separate Pyricularia oryzae genomic DNA by pulse-field gel electrophoresis (PFGE).  相似文献   

10.
4183 is a promising wide compatibility restorer line with good grain quality. Its hybrid rice Shuangyou 4183 (Shuangjiu A/ 4183) was registered in Anhui Province in 2003. However, the line and its hybrid rice are susceptible to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo). R4183 carrying Xa21 was developed to improve bacterial blight resistance of 4183 through introducing the broad-spectrum resistance gene Xa21 by marker-aided selection. R4183 had similar level of bacterial blight resistance to IRBB21, the resistant donor, while maintained the wide compatibility, restoring ability and other good economic traits of the recurrent parent 4183. Critical issues on improvement of bacterial blight resistance of hybrid rice and breeding strategies were also discussed.  相似文献   

11.
Many fungi have hyphal fusion ability, including rice blast fungus [ Magnaporthe grisea( Hebert ) Barr nov. Comb, ( Anamorph Pyricularia oryzae Cavara)], and which is one of the major causes to pathogenic variation.Santo H has obserced the phenomenon of hyphal fusion in rice plant under electron rnieroscope and isolated some races which were different from parental ones.  相似文献   

12.
Marker-aided selection has received more attention in recent years. This relies on the exploitation of close linkage between molecular markers and target gene(s). We report here a randomly amplified polymorphic DNA (RAID) marker tightly linked to the blast resistance gene Pi-11(t) derived from Hongjiaozhan, which confers the resistante to race ZBI of Pyricularia oryzae Car.  相似文献   

13.
Identification of immunity-associated leucine-rich repeat receptor-like protein kinases(LRR-RLK) is critical to elucidate the LRR-RLK mediated mechanism of plant immunity. Here, we reported the map-based cloning of a novel rice SPOTTED-LEAF 41(Os SPL41) encoding a putative LRR-RLK protein(Os LRR-RLK41/Os SPL41) that regulated disease responses to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae(Xoo). An 8-bp insertion at position 865 bp in a mutant spotted-leaf 41(spl41) allele led to...  相似文献   

14.
We report, in this paper, some results of in vitro selection of rice cell lines resistant to raw toxin extract of P. oryzae.  相似文献   

15.
Bacterial streak(BS) caused by Xanthomonas campesiris pv. oryzicola bas become one of the major diseases in southbern rice areas.Up to date, tbere are few reports on the inheritance of resistance to BS and its relationsbip with the resistance to bacterial blight(BB).  相似文献   

16.
Bacterial streak(BS)caused by Xanthomonascampestris pv.oryzicola has become one of themajor diseases in southern rice areas.Up todate,there are few reports on the inheritanceof resistance to BS and its relationship with theresistance to bacterial blight(BB).Therefore,we analyzed the inheritance of resistant to BSin three resistant rice cultivars and the genetic  相似文献   

17.
Osa-miR439 is a rice-specific microRNA family. Here we showed that Osa-miR439 acted as anegative regulator in rice immunity against blast fungus Magnaporthe oryzae. Osa-miR439 differentiallyresponded to M. oryzae between susceptible and resistant rice accessions. The accumulation ofOsa-miR439 was constitutively more in the susceptible accession than in the resistant one. Transgeniclines overexpressing Osa-miR439a (OX439a) showed higher susceptibility associating with lower inductionof defense-related genes and less hydrogen peroxide (H2O2) accumulation at the infection sites than thecontrol plants. In contrast, transgenic lines expressing a target mimic of Osa-miR439 (MIM439) displayedcompromised susceptibility associating with increased H2O2 accumulation. Furthermore, we found thatthe expression of three predicted target genes was decreased in OX439a but increased in MIM439 incomparison to control plants, and this expression was differential in susceptible and resistant accessionsupon M. oryzae infection, indicating that Osa-miR439a may regulate rice blast resistance via these genes.Our results unveiled the role of Osa-miR439a in rice blast resistance and provided the potentiality toimprove the blast resistance via miRNA.  相似文献   

18.
It is widely believed that a rice variety loses its resistance to rice blast after three to five years in commercial production due to the emergence of new Magnaporthe oryzae pathotypes(or physiological races)(Ou,1985).However,we believe that a resistant rice variety loses its resistance to M.oryzae not due to the emergence of new physiological races.We propose that pathogenic physiological races already exist in the natural environment,serving as potential physiological races.It is only because of the lack of appropriate environmental conditions,particularly the lack of a suitable rice host,on which such a race is unable to propagate widely and exists as an inferior race.However,when there is an appropriate rice host,the potential pathogenic physiological race can quickly proliferate to become the dominant physiological race,thus leading to the loss of blast resistance in resistant rice varieties.  相似文献   

19.
Rice bacterial blight（BB）resistance has been amajor target in rice breeding programs.How-ever,the result of resistance identificationwith traditional method is easily affected byenvironmental factors.This problem would besolved through marker assisted selection.Sofar,most of the known BB resistance genes,such as Xa-1,Xa-2,Xa-3,Xa-4,xa-5,Xa-  相似文献   

20.
The interaction between rice host and its pathogen Xanthomonas oryzae pv. oryzae (Xoo) at cellular level was studied by using a resistant somaclonal mutant HX-3 and its susceptable donor Minghui 63. After inoculation with Xoo strain Zhe 173 (Chinese pathotype |\ ). the activity of superoxide dismutase (SOD) and peroxidase (POD) in the callus of Minghui 63 was increased dramatically, and the active oxygen(O2^-) was produced at a higher rate; Meanwhile, the callus grew slowly with the reduction of protein content. Compared to the activity of SOD and POD. the production rate of O2^- and the fresh weight in HX-3 callus varied little after the inoculation. It could be proposed that there were great differences between the resistance of HX-3 and Mighui 63 at cellular level. There was no difference detected concerning resistance to bacterial leaf blight in HX-3 between the plant and the callus.  相似文献   

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