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1.
Contamination of food and water by microorganisms from animal manure has become an important issue in public health. Escherichia coli O157:H7 is one of several emerging pathogens of concern. In this research, we studied how the self-heating, thermophilic phase of composting influenced laboratory-grown vs. bovine-derived E. coli O157:H7 mortality, specifically the relationship between temperature, time at temperature, and pathogen survival. Composting experiments were conducted in laboratory-scale bioreactors operated in three temperature ranges: 40°C to 50°C, 50°C to 60°C, and greater than 60°C. We measured the effects of temperature and composting time on E. coli O157:H7 mortality. Laboratory-grown E. coli O157:H7, inoculated into the initial compost material, were not detected after approximately 300 degree days of heating. In several experiments where compost temperatures did not rise above 50°C, an initial decline of E. coli O157:H7 with subsequent regrowth was observed. E. coli O157:H7 in compost materials from infected cattle were not detected after approximately 180 degree days of heating. Numbers of total coliform bacteria declined with temperature similarly to those of E. coli O157: H7. The results of this research provide information for reducing or eliminating E. coli O157:H7 in animal wastes.  相似文献   

2.
《Applied soil ecology》2011,47(3):398-404
The inactivation of Escherichia coli O157:H7 (CCUG 44857) and Salmonella enterica serovar Typhimurium was investigated in two agricultural soils (sandy loam and silty clay) amended with poultry manure, cattle manure slurry or human urine. The study was performed in soil lysimeters placed outdoors, and was repeated over two consecutive years. The amendments, inoculated with E. coli O157:H7 and Salmonella Typhimurium, were mixed with soil on the top of the lysimeters. Samples were collected from the top 5-cm layer of each lysimeter at regular intervals, and the inactivation was monitored over 6 months, by the plate spread method and by enrichment. The inactivation was modelled by fitting a non-linear model to the data, and pathogen reduction times were calculated (90 and 99% reduction). The results showed that the inactivation of E. coli O157:H7 and Salmonella Typhimurium varied depending on the manure type used and its carbon content. The longest inactivation time occurred in samples amended with poultry manure, in which both E. coli O157:H7 and Salmonella Typhimurium were detected up to day 90 with the spread plate method. The most rapid inactivation for both pathogens occurred in soil amended with urine. However, low amounts of culturable E. coli O157:H7 and Salmonella Typhimurium were detected by enrichment throughout the study period (180 days), regardless of manure type.  相似文献   

3.
The risk of enteropathogens to food and water is highly dependent on their survival in soil environments. Here, the effects of soil type, particle size, the presence of natural organic matter (NOM) or Fe/Al (hydro)oxides on pathogenic Escherichia coli O157:H7 survival in sterilized soil particles were assessed through survival, attachment, metabolic activity, and qRT-PCR analyses. The abundance of inoculated E. coli O157:H7 in Brown soil (Alfisol) particles increased 0.6–1.4 log10 CFU/g within 3 days (except for NOM-stripped clay), while that in Red soil (Ultisol) particles decreased rapidly in 8 days post-inoculation. Additionally, survival of bacteria was significantly enhanced when Fe/Al (hydro)oxides had been removed from Red soil particles. For the two soils, E. coli O157:H7 survived the longest in NOM-present clays and the bacterial adenosine 5′-triphosphate (ATP) levels were 0.7–2.0 times greater in clays than in sands and silts on day 8. Moreover, clays were more effective than silts and sands in binding cells and changing the expressions of acetate pathway-associated genes (pta and ackA). For silts and sands, E. coli O157:H7 decayed more rapidly in the presence of NOM and similar trends of bacterial ATP levels were observed between NOM-stripped and NOM-present soil particles, indicating that the primary role of NOM was not as a nutrient supply. These findings indicate that soil particles function mainly through attachment to change the metabolic pathway of E. coli O157:H7 and ultimately impact the survival of bacterial pathogens in soils.  相似文献   

4.
Escherichia coli O157:H7 contaminated wastes such as animal manures and abattoir wastes, may be applied directly onto vegetation, the soil surface, or injected deep into the soil. Our aim was to determine the influence of method of waste application to land on E. coli O157:H7 survival. Bovine slurry and ovine stomach contents containing E. coli O157:H7 were applied to the surface vegetation or sub-surface injected at 25 cm below the soil surface. E. coli O157 survived but did not proliferate on grassland vegetation for up to 6 weeks and in the underlying soil for 8 weeks. Our results suggest that sub-surface injection of organic wastes into soil may reduce the risk of pathogen persistence in the environment.  相似文献   

5.

Purpose

The Escherichia coli (E. coli) O157:H7 survival dynamics in original and pH-modified agricultural soils were investigated to determinate how E. coli O157:H7 survival responded to the pH values of different soils, identify the relationships between E. coli O157:H7 survival time (t d ) and soil properties, and assess the potential pathogen contamination after soil pH changed.

Materials and methods

The six soil samples were collected from different provinces of China, and 18 pH-modified soil samples were obtained from original soils by treating the original soils with direct electric current. The E. coli O157:H7 cells were inoculated into 24 soils and incubated at soil moisture of ?33 kPa and 25 °C. The soils were sampled for determining the numbers of E. coli O157:H7 at given time intervals over the incubation. The effects of soil pH change and other properties on the t d values were analyzed.

Results and discussion

The t d values in the test soils were between 7.1—24.7 days. Results indicate that soil pH, texture, and free Fe2O3 (Fed) were the most important factors impacting the t d values in the test soils. Further, the response of E. coli O157:H7 survival to pH change varied with different soils. In the acidic soils (shorter t d values), the t d values decreased as the pH decreased and Fed increased, while in the neutral or alkaline soils (pH?≥?6.45, longer t d values), the t d values did not change significantly with pH.

Conclusions

The changes of amorphous and free sesquioxides induced by pH change might strengthen the response of E. coli O157:H7 survival to soil pH. Closer attention should be paid to E. coli O157:H7 long survival in soils and its potential environmental contamination risk.  相似文献   

6.
The population dynamics of Salmonella enterica var. Typhimurium MAE 110 gfp, Escherichia coli O157:H7 gfp, and Pseudomonas fluorescens 32 gfp were investigated in their introduction to cattle excrements and subsequent entering the soil, plants of cress (Lepidium sativum L.), and migration through the gastroenteric tract of French snails (Helix pomatia L.). The survival of these bacteria in the excrements and soil was investigated at cyclically changing (day-night, 25–15 °C) and constant (18 °C) temperatures. The cyclically changing temperature adversely affected the survival of E. coli O157:H7 gfp, and P. fluorescens but did not influence S. enterica var. Typhimurium. All the bacteria and, especially, the analogues of enteropathogens showed high survival in the cattle and snail excrements, soil, and on the plants under the gradual decrease in their population. On the cress plants grown in a mixture of cattle excrements and soil, an increase in the number of the introduced bacteria was observed.  相似文献   

7.
Abstract. Coliforms such as Escherichia coli and E. coli O157 are present in faeces deposited on the ground by grazing livestock, which gives rise to environmental concerns about the consequences of their transport in soil water draining to rivers, lakes, groundwater, water supplies and bathing waters. Following a similar study in relation to slurry spreading (Soil Use and Management 2003; 19, 321–330), a two‐stage approach was adopted to using the dual‐porosity contaminant transport model macro to simulate processes by which E. coli microorganisms from grazing livestock (sheep) pass through the soil to receiving waters via field drains. First, model parameter values were selected to reproduce experimental measurements showing rapid flows of the organisms by macropore flow without trapping in smaller pores. However, because of the large number of parameters and likely experimental errors, the set of values chosen, although plausible, is not necessarily unique and so any predictions should be considered provisional pending validation. Second, a series of predictive simulations was carried out to test the influence of soil and weather conditions on losses to field drains during grazing. These showed that E. coli losses were influenced almost entirely by the soil water content at the time of grazing, rising to a high level during grazing in wet conditions, but low or zero under dry conditions. In contrast, rainfall at the time of grazing had almost no consistent effect, other than large losses on the occasional days with over 20 mm of rain. Overall losses for a period of grazing were generally small during summer, but rose to a high level if grazing continued into autumn, due to the increase in soil water content. This demonstrates that there would probably be substantial reductions in the environmental risks of water pollution by E. coli and other faecal microorganisms if continuous grazing were stopped around early September and replaced by grazing on dry days only.  相似文献   

8.
Multiple outbreaks of food poisoning associated with fresh vegetable consumptions have occurred in many countries. Numerous reports have described human pathogenic bacteria, such as Escherichia coli O157:H7 and Salmonella spp., that can internalize into fresh vegetables via root or leaf surfaces. While attempting to obtain the threshold concentration of internalization of E. coli inoculated into hydroponic medium during vegetable cultivation, we observed a rapid decrease in E. coli numbers. In the present study, we determined that the rapid decline in E. coli was not due to a physiological change into a viable but non-culturable (VNC) state. The population crash was instead caused by true bacterial death, as the rapid descent was also confirmed by micro-colony fluorescence in situ hybridization, a culture-independent method that can detect VNC cells. We next monitored the number of E. coli inoculated into intact or filter-sterilized hydroponic medium after cultivation of various types of plants. We found that the number of E. coli in intact hydroponic medium decreased markedly, whereas the level in filter-sterilized hydroponic medium was completely unchanged. This result suggests that biotic factors were present that could be eliminated by filtering. Robust predation of E. coli by protozoa (ciliates and flagellates) was observed using fluorescently labeled bacteria incorporated into the hydroponic medium. Finally, morphological identification of flagellates by scanning electron microscopy revealed the presence of a species of Stramenopiles. These findings suggest the importance of protozoa as bacterial feeders in hydroponic systems and hence the use of these organisms as potential control agents of human pathogenic bacteria.  相似文献   

9.
Compost water extracts (compost teas) are gaining popularity among organic growers, largely because of their disease suppressive activity when applied to foliage or soil. Production methods often include addition of supplemental constituents, particularly molasses, to stimulate plant-beneficial microbial populations. We have found that molasses amendments also favor regrowth of human pathogenic bacteria, raising public health concerns about potential contamination of treated crops, particularly produce intended for fresh consumption. Using disease outbreak strains marked with green fluorescent protein (GFP) and spontaneous antibiotic-resistance, we found that regrowth of Salmonella enterica serovar Thompson and Escherichia coli O157:H7 was positively correlated with molasses concentration. For Salmonella, regrowth was also dependent on the type of starter compost material used. Salmonella populations increased from 1 at time 0 to over 1000 CFU ml?1 in dairy manure compost tea with 1% molasses, and from 1 at time 0 to over 350,000 CFU ml?1 in chicken manure compost tea by 72 h. E. coli populations increased from 1 at time 0 to approximately 1000 CFU ml?1 in both types of tea by 72 h. Pathogen regrowth did not occur when molasses was eliminated or kept to 0.2%.  相似文献   

10.
Infections caused by waterborne Escherichia coli O157 continue to pose a public health risk. An increase in faecal coliform loading of watercourses due to expanding populations, intensification of agriculture and climate change are predicted to amplify these risks. Understanding the effect of land use on the ecology of E. coli O157 in environmental waters is therefore important for implementing effective mitigation measures. In order to test the hypothesis that activity of waterborne E. coli O157 is affected by both land use type and the respective autochthonous microbial communities, we inoculated replicate microcosms of water collected from areas of contrasting land uses within a catchment with a chromosomally lux-marked E. coli O157. Pathogen metabolic activity and its ability to reactivate following addition of nutrients were quantified over time in both filter-sterilised and non-sterile microcosms. Metabolic activity differed significantly according to the land use type, the degree of competition from background microbes and the availability of nutrients. These results indicate that land use types associated with particular areas of a watercourse should be considered a central factor in models that aim to predict pathogen risk in environmental waters.  相似文献   

11.
Humic acids are ubiquitous and abundant in terrestrial environments; therefore, they are often co-extracted with nucleic acids and interfere with quantitative PCR (qPCR) assays. In this study a recently developed NanoGene assay that is resistant to interference by humic acids was evaluated for gene detection in soil samples. The NanoGene assay utilizes a combination of magnetic beads, dual quantum dots labels, and DNA hybridization in solution. Seven soil samples containing different amounts of organic matter were tested to compare NanoGene and qPCR assays for their respective ability to detect a bacterial pathogen. We spiked the soils with Escherichia coli O157:H7, extracted genomic DNA, and conducted NanoGene and qPCR assays targeting the E. coli O157:H7-specific eaeA gene. To prevent the inhibition of PCR that is common when using DNA extracted from soils, we used a range of template DNA concentrations and BSA addition in the qPCR assay. Compared to the qPCR assay the NanoGene assay was significantly more resistant to the inhibitory effect of humic acids, successfully quantifying the eaeA gene within a linear (R2 = 0.99) range of 105 through 108 CFU/g soil for all seven soil samples tested. In contrast, the qPCR assay was significantly inhibited using the same template DNA isolated from soils containing a range of organic content (2.0%–12%). Interestingly, the qPCR assay was still inhibited despite additional purification steps, suggesting that humic acids were still associated with DNA at a level that was inhibitory to qPCR. This study demonstrated that the NanoGene assay is suitable for quantitative gene detection in diverse soil types and is not susceptible to inhibition by humic acids and other organic compounds that commonly lead to false negative results in qPCR assays.  相似文献   

12.
病原菌在红壤胶体上的吸附机制研究   总被引：1，自引：0，他引：1  
赵文强  刘星  蔡鹏  黄巧云 《土壤学报》2013,50(2):221-229
研究了pH和KCl离子强度对猪链球菌和大肠杆菌在红壤胶体表面吸附的影响,结合表面物化性质和Derjaguin-Landau-Verwey-Overbeek(DLVO)理论分析互作机制。结果表明,细菌在红壤胶体表面的吸附等温线能较好拟合Freundlich方程(R20.97),猪链球菌在红壤胶体表面吸附的分配系数(Kf)是大肠杆菌的4.5倍～6.1倍,细菌在去有机质胶体表面吸附的Kf值为含有机质胶体的2.4倍～3.2倍。比表面积越大或zeta电位绝对值越小,细菌吸附能力越强,吸附态细菌位于距红壤胶体表面90～100 nm处的次级小能位置。随着体系pH降低(9.0～4.0)或离子强度增大(1～10 mmol L-1),细菌与红壤胶体互作能障降低,细菌吸附量增大,吸附机制符合DLVO理论;而在高离子强度下(50～100 mmol L-1),猪链球菌吸附量降低了3.4%～5.6%,表明除DLVO作用力外,非DLVO作用力如空间位阻排斥和疏水作用对吸附也有贡献。  相似文献   

13.
The adsorption and binding of plasmid p34S DNA on four different colloidal fractions from a Brown soil and clay minerals in the presence of various Ca2+ concentrations, the ability of bound DNA to transform competent cells of CaCl2-treated Escherichia coli, and the resistance of bound DNA to degradation by DNase I were studied. DNA adsorption on soil colloids and clay minerals was promoted in the presence of Ca2+. Kaolinite exhibited the highest adsorption affinity for DNA among the examined soil colloids and clay minerals. In comparison with organo-mineral complexes (organic clays) and fine clays (<0.2 μm), DNA was tightly adsorbed by H2O2-treated clays (inorganic clays) and coarse clays (0.2-2 μm). The transformation efficiency of bound DNA increased with increasing concentrations of Ca2+ at which soil colloid or clay mineral-DNA complexes were formed. DNA bound by kaolinite showed the lowest transformation efficiency, and especially no transformants were observed with kaolinite-DNA complex prepared at 5-100 mM Ca2+. Compared to organic clays and fine clays, DNA bound on inorganic clays and coarse clays showed a lower capacity to transform E. coli at different Ca2+ concentrations. The presence of soil colloids and minerals provided protection to DNA against degradation by DNase I. Montmorillonite, organic clays and fine clays showed stronger protective effects for DNA than inorganic clays and coarse clays. The protection mechanisms as well as the differences in transforming efficiency of plasmid DNA molecules bound on various soil colloidal particles are discussed. The information obtained in this study is of fundamental significance for the understanding of the horizontal dissemination of recombinant DNA and the fate of extracellular DNA in soil environments.  相似文献   

14.
During aerobic composting of animal manure, microbial activity within the mixture generates heat and metabolic by-products that inactivate zoonotic pathogens. Although it is recognized that the type and level of microbial activity will vary with the nutrient availability of different compost ingredients, the degree to which these changes could impact pathogen inactivation is of interest. Towards that goal, the purpose of this study was to determine inactivation of Escherichia coli O157:H7 and Listeria monocytogenes in compost laboratory-scale bioreactor systems formulated to different initial carbon:nitrogen (C:N) ratios with cow manure, straw, and cottonseed meal. The C:N ratio did not significantly affect the time to inactivation of Listeria monocytogenes. In contrast, Escherichia coli O157:H7 survived for significantly (P < 0.05) longer periods of time in 40:1 C:N systems than in 30:1 or 20:1 systems even though the cumulative heat exposures were statistically similar in the systems. An increase of pH values to 8 to 9 occurred initially for 40:1 C:N systems, whereas the pH of the 20:1 and 30:1 systems initially declined to 5.5 to 6 before increasing to 8 to 9 after 2 days of composting.  相似文献   

15.
Abstract. This article examines some of the factors that influence the relative risk of Escherichia coli pollution of surface waters from grazing animals compared to cattle slurry application. Drainage water from pipe‐drained plots grazed with sheep (16 sheep + lambs per hectare) from 29 May to 17 July 2002 had average E. coli counts of 11 c.f.u. mL?1 or 0.4% of estimated E. coli inputs over the grazing period. Drainage water from plots on the same site treated with cattle slurry (36 m3ha?1 on 29 May 2002) had lower average E. coli counts of 5 c.f.u. mL?1 or 0.03% of estimated faecal input. Sheep (16 lambs per hectare) grazing under cooler, moister conditions from 24 September to 3 December 2001 gave drainage water with much higher average E. coli counts of 282 c.f.u. mL?1 or 8.2% of estimated input, which is more than twice the average E. coli counts previously reported under such conditions (Vinten et al. 2002 Soil Use and Management 18, 1–9). Laboratory studies of runoff from soil slabs after slurry application showed that the mobility of E. coli in surface soil decreased with time, suggesting that increased attachment to soil or migration to ‘immobile’ water also provides at least part of the physical explanation for the relatively higher risk of pollution from grazing animals compared with slurry. Sampling for E. coli in field drainflow and in streamwater during a storm event in the predominantly dairy Cessnock Water catchment, Ayrshire, Scotland supported the hypothesis that E. coli transport is linked to grazing animals. For a 7‐mm rainfall event, roughly 14% of the estimated daily input from grazing livestock was transported to the river, even though little slurry spreading had occurred in the catchment in the previous month. Spot sampling of field drains in grazed fields and silage fields in the same catchment also showed that grazing animals were the principal source of E. coli and faecal streptococci.  相似文献   

16.
The effects of time and temperature on the release kinetics of water‐dispersible colloids (WDCs) from three German silt loam topsoils in deionized water were investigated in batch experiments under low‐energy rotating shaking conditions. The measured critical coagulation concentrations of Ca2+ and Na+ for extracted WDC were much larger than the experimental ionic conditions. This indicates a fast dispersion rate in the first detachment step of WDC mobilization from soil aggregates. The cumulative released WDC fraction F(t) (released WDC/clay content in bulk soil) was satisfactorily fitted to the square root of shaking time by a linear function in three soils with a similar clay content. This implies diffusion‐controlled release kinetics in the second step of the WDC mobilization process. The mobilization kinetics were modelled by considering a diffusion‐controlled transport through an immobile water layer in the macropores of soil aggregates formed by silt and sand particles. The effects of temperature on the mobilization kinetics and sedimentation volumes of saturated soils were compared at 7, 23 and 35°C. A linear correlation was found between immobile water layer thickness in soil macropores (lt) and the water volume (Vwater) in soil sediment, which indicates a strong dependence of lt on the soil texture. Temperature‐sensitive lt and Vwater influenced the effect of temperature on WDC release, which counteracts the estimated effect of temperature on particle diffusion according to the Stokes‐Einstein relation. A larger decrease in F(t) was found in grassland and forest soils than in an arable soil and can be related to greater stagnant water contents (larger lt and Vwater) in soil macropores, where particulate organic matter and polyvalent cations in their oxide forms at acidic pH will thus contribute to water immobilization.  相似文献   

17.

Purpose

Colloid migration in subsurface environments has attracted considerable attention in recent years because of its suspected role in facilitating transport of strongly adsorbed contaminants to groundwater. The influence of bulk solution pH or ionic strength on model colloid (i.e., latex microsphere, amorphous silica colloids) transport is well established, while little attention has been paid to water-dispersible soil colloids. In this study, saturated packed columns were conducted to explore the mechanism of transport and fate of water-dispersible soil colloids and facilitating transport of Cu during transients in solution chemistry.

Materials and methods

Water-dispersible soil colloids were fractionated from a Cu-contaminated soil sample. Transport of soil colloidal suspensions was conducted with varying pH and ionic strengths, and then, re-entrainment of those retained colloids after completion the transport experiments was conducted by changing pore water solution transient ionic strength and pH conditions. Meanwhile, transport and fate of the Cu strongly adsorbed on the soil colloids were determined under different ionic strength conditions.

Results and discussion

The transport behavior of soil colloids in porous media was found to depend on the pH and ionic strengths of bulk solution. An increase in solution ionic strength and decrease in solution pH resulted in greater deposition which was revealed by the collision efficiency (??). It increased from 0.15 to 1.0 when solution composition changed from 0 to 50?mM NaNO3 and decreased dramatically from 1.0 to 0.035 as the solution pH converted from 2.97 to 8.94. The results were in agreement with Derjaguin?CLandau?CVerwey?COverbeek theory. Upon stepwise reduction in ionic strength of eluting fluid or enhancement in its pH, a sharp release of colloids retained in the column occurred in each step. Meanwhile, the value of FRE NaOH that reveals the effect of NaOH solution at pH?11 on the mobilization of retained colloids deposited in the primary minimum increased from 38.6% to 64.6% when the ionic strength of bulk solution changed from 0 to 50?mM NaNO3 and decreased from 86.7% to 35.8% as the solution pH from 2.97 to 8.94. In addition, the transport and fate of the Cu strongly adsorbed on soil colloids were highly consistent with the results of soil colloids.

Conclusions

The colloid collision efficiency (??) decreased as the pH of bulk solution increased and increased as the ionic strength of bulk solution increased in saturated columns packed with pure quartz sand, and NaOH solution at pH?11 poses a predominant role on mobilization of the retained colloids deposited in the primary minimum. Meanwhile, the strongly adsorbed Cu on soil colloids almost cannot be detached from its carrier under the competition of coexisted cations in the bulk solution and cotransport with its carrier under different ionic strengths.  相似文献   

18.
Recently, there has been a surge of interest in understanding the survival of Shiga toxin-producing Escherichia coli (STEC) in aquatic environments. Fifteen strains of STEC were monitored, individually, in untreated well water samples incubated at 10 and 22^C for 56 days. The strains were selected from three serogroups (O26, Ol11 and O157) and represented five distinct ERIC (enterobacterial repetitive intergenic concensus)-genotypes. The microcosms were prepared in triplicate and inoculated at an initial cell density of about 7.0 log CFU/ml well water. At 10^C, the cell density of five STEC strains fell below the detection limit of 0.8 log CFU/ml by day 56. Of the ten persisting strains, four showed superior survival with cell densities decreasing to an average of about 5 log CFU/ml while the remaining six strains showed moderate levels of survival, decreasing to an average cell density of about 3 log CFU/ml. At 22^C, strain H32 (genotype I) and H15 (genotype B) persisted at 1.1 log CFU/ml and 2.2 log CFU/ml in 56 days, respectively. The other 13 STEC strains dropped below the detection limit between weeks 3 to 8. The 15 strains demonstrated highly variable levels of survival with no correlation between ERIC-genotypes or serogroups and the strains' ability to persist in the well water samples. Although strain H32 (O157:H7) persisted significantly longer than strain H22 (O157:H7) in natural well water at both 10 and 22^C, both strains survived equally well in sterile well water, indicating that individual STEC strains vary in their ability to compete with background microbial populations.  相似文献   

19.
草萘胺在五种土壤及其胶体上的吸附行为   总被引：1，自引：1，他引：0  
何任红  马爱军 《土壤》2011,43(6):993-997
土壤胶体是土壤中颗粒最细小的部分,由于具有巨大的比表面积和较高的吸附容量,是环境中污染物的重要吸附剂,直接影响到污染物的归趋和生物有效性.本文采用批次吸附试验的方法,研究了农药草萘胺在黄棕壤等5种土壤及土壤胶体上的吸附行为.结果表明,草萘胺在供试土壤及胶体上的吸附均能很好地符合Freundlich方程;与本体土壤相比,...  相似文献   

20.
Ovomucin glycopeptide (OGP) was prepared by size exclusion chromatography after Pronase digestion of hen egg ovomucin, and the binding of OGP to foodborne pathogens (Bacillus cereus,Clostridium perfringens, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, and Staphylococcus aureus) was investigaed. Binding assays with biotinylated bacteria as probes in microtiter plates showed that OGP bound to only E. coli O157:H7 among these foodborne pathogens. Periodate treatment markedly reduced the binding ability, indicating that E. coli O157:H7 bound to carbohydrate moieties of OGP. Lectin blot analysis with Maackia amurensis (MAA) and Sambucus nigra (SNA), which are specific for oligosaccharides containing sialic acid, revealed their binding sites in OGP were similar to the E. coli O157:H7 binding sites that were probed with biotinylated E. coli O157:H7 after Western blotting of OGP. Sialydase treatment of OGP abolished its ability to bind E. coli O157:H7, demonstrating that sialic acid played an important role in the binding. These results suggest that OGP has E. coli O157:H7-specific binding sites that consist of sialic acid. On the basis of these properties, OGP has the potential to be an ingredient with a protective effect against E. coli O157:H7 infection and to be a novel probe for the detection of E. coli O157:H7 in the food hygiene field.  相似文献   

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