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1.
本试验旨在研究1~21日龄爱拔益加(AA)×罗曼肉杂鸡饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸的适宜水平。采用L_9(3~4)正交试验设计,饲粮代谢能水平分别为11.70、12.12、12.54 M J/kg;粗蛋白质水平分别为19.00%、20.00%、21.00%;蛋氨酸水平分别为0.45%、0.50%、0.55%;赖氨酸水平分别为1.00%、1.10%、1.20%。选取1日龄AA×罗曼肉杂鸡864只,随机分为9组,每组6个重复,每个重复16只,试验期21 d。通过饲养试验、屠宰试验等方法检测不同营养水平饲粮的饲喂效果。结果表明:1)饲粮粗蛋白质、蛋氨酸和赖氨酸水平对试验鸡平均日增重有显著影响(P0.05),21.00%粗蛋白质水平组显著高于20.00%粗蛋白质水平组(P0.05),0.50%蛋氨酸水平组显著高于0.55%蛋氨酸水平组(P0.05),1.20%赖氨酸水平组显著高于1.10%赖氨酸水平组(P0.05)。2)饲粮代谢能水平对21日龄试验鸡活体总能含量有显著影响(P0.05),12.12 MJ/kg代谢能水平组显著高于12.54 MJ/kg代谢能水平组(P0.05);饲粮蛋氨酸水平对试验鸡活体粗脂肪含量有显著影响(P0.05),0.50%和0.55%蛋氨酸水平组显著高于0.45%蛋氨酸水平组(P0.05)。3)饲粮代谢能和粗蛋白质水平对21日龄试验鸡活体能量沉积率有显著影响(P0.05),11.70和12.12 MJ/kg代谢能水平组显著高于12.54 MJ/kg代谢能水平组(P0.05),21.00%粗蛋白质水平组显著高于20.00%和19.00%粗蛋白质水平组(P0.05);1.10%赖氨酸水平组的试验鸡活体赖氨酸沉积率显著高于1.20%赖氨酸水平组(P0.05)。4)饲粮赖氨酸水平对试验鸡血清葡萄糖、尿酸、尿素氮水平有显著影响(P0.05),1.00%赖氨酸水平组血清葡萄糖水平最高,显著高于1.20%赖氨酸水平组(P0.05),1.10%赖氨酸水平组血清尿酸水平最高,显著高于1.20%赖氨酸水平组(P0.05),1.20%赖氨酸水平组血清尿素氮水平最高,显著高于1.10%赖氨酸水平组(P0.05)。5)由回归分析得出1~21日龄AA×罗曼肉杂鸡饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸适宜水平的计算公式分别为ME=45.33W~(0.75)+183.84△W,CP=19.77 W~(0.75)+626.47△W,Met=1.44 W~(0.75)+10.31△W,Lys=3.01 W~(0.75)+21.28△W(M E为代谢能,W~(0.75)为代谢体重,△W为平均日增重,CP为粗蛋白质,Met为蛋氨酸,Lys为赖氨酸),根据公式计算得出饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸的适宜水平分别为11.90 MJ/kg、21.08%、0.51%和1.05%。 相似文献
2.
本试验旨在研究育成期京红1号商品代母鸡的饲粮赖氨酸(Lys)需要量。选取450只8周龄京红1号商品代母鸡,随机分成5组,每组6个重复,每个重复15只鸡。分别饲喂Lys水平为0.56%、0.62%、0.68%、0.74%和0.80%的饲粮,试验期为9周。结果表明:随饲粮Lys水平的增加,9~17周龄京红1号母鸡的平均日增重(ADG)和群体均匀度均呈显著先升高再降低的二次曲线关系(P0.05),料重比(F/G)呈显著先降低再升高的二次曲线关系(P0.05);0.62%Lys组的ADG显著高于0.56%和0.80%Lys组(P0.05),F/G却显著低于以上2组(P0.05),0.68%Lys组的群体均匀度显著高于0.56%和0.80%Lys组(P0.05)。17周龄京红1号母鸡的体重、胸宽和胫长随饲粮Lys水平的增加均呈显著先升高再降低的二次曲线关系(P0.05);0.68%Lys组的体重、胸宽和胫长均显著高于0.74%和0.80%Lys组(P0.05)。17周龄京红1号母鸡的卵巢重量、卵巢指数、输卵管重量、输卵管指数及输卵管长度随饲粮Lys水平的增加呈显著先升高再降低的二次曲线关系(P0.05);0.68%Lys组的生殖器官发育指标均显著高于其他组(P0.05)。随饲粮Lys水平的增加,17周龄京红1号母鸡的卵巢中各级卵泡数均呈显著先增加后降低的二次曲线关系(P0.05);0.68%Lys组的总卵泡数、初级卵泡数、次级卵泡数和小黄卵泡数均显著高于其他组(P0.05)。根据17周龄京红1号母鸡的卵巢重、卵巢指数、总卵泡数、初级卵泡数和次级卵泡数各自拟合二次曲线,得出饲粮Lys需要量分别为0.65%、0.65%、0.64%、0.64%和0.64%。综上所述,推荐9~17周龄京红1号母鸡饲粮Lys需要量为0.64%~0.65%。 相似文献
3.
本试验旨在研究京红商品代蛋鸡预产蛋期(17~20周龄)饲粮蛋氨酸(Met)需要量。选取16周龄末京红商品代蛋鸡750只,随机分为5组,每组5个重复,每个重复30只鸡。各组饲粮Met水平分别为0.20%、0.26%、0.32%、0.38%、0.44%,试验期4周。结果表明:随着饲粮中Met水平的提高,各组试验鸡20周龄末体重(FBW)和平均日增重(ADG)呈线性增加(P0.05),0.44%Met组的FBW和ADG最高;饲粮Met水平可显著影响血清游离Met含量,0.44%Met组血清游离Met含量显著高于0.20%、0.26%和0.32%Met组(P0.05);日粮Met水平显著影响血清尿酸(UA)含量,0.44%和0.38%Met组的血清UA含量显著高于0.20%和0.26%组(P0.05);随着日粮Met水平的提高,血清碱性磷酸酶(ALP)活性线性增加(P0.05),0.44%Met组ALP活性最高,与其他各组差异不显著。在本试验条件下,京红商品代蛋鸡预产蛋期(17~20周龄)Met需要量为0.44%。 相似文献
4.
本试验旨在研究不同蛋氨酸(Met)水平对13~16周龄京红商品代蛋鸡生长性能以及后续产蛋性能的影响。选用12周龄末体重胫长相近的750只京红商品代蛋鸡,随机分为5组,每组5个重复,每个重复30只鸡。对照组饲喂Met水平为0.18%的玉米-豆粕型基础饲粮,试验组在基础饲粮水平上用0.06%、0.12%、0.18%、0.24%的Met替代等量的膨润土,使其Met水平分别达到0.24%、0.30%、0.36%、0.42%,试验期4周。饲养试验结束后统一饲粮继续观察至27周。结果表明:Met水平对末体重、平均日增重、平均日采食量和耗料增重比无显著影响(P0.05);0.30%和0.42%Met组血清中总蛋白、白蛋白和球蛋白显著高于0.18%Met组(P0.05);饲粮Met水平对血清白球比、碱性磷酸酶活性、尿酸和血氨含量无显著影响(P0.05);0.42%Met组血清中游离Met含量显著高于0.18%、0.24%、0.30%Met组(P0.05);在后续观察中,0.36%Met组京红蛋鸡的体重均匀度最高,且显著高于0.30%Met组(P0.05),后期产蛋性能高于0.42%Met组。综合分析,京红商品代蛋鸡13~16周龄Met需要量为0.36%。 相似文献
5.
本试验旨在研究5~8周龄京红1号蛋鸡饲粮赖氨酸(Lys)的需要量。选取4周龄末京红1号蛋鸡母雏360只,随机分为5组,每组6个重复,每个重复12只鸡。饲粮Lys水平分别为0.70%、0.84%、0.98%、1.12%和1.26%,试验期4周。结果表明:0.84%Lys组蛋鸡平均日增重、8周龄体重和群体均匀度均显著高于1.26%Lys组(P0.05)。0.98%和1.12%Lys组的体格发育显著优于1.26%Lys组(P0.05)。0.98%和1.12%Lys组的十二指肠重、空肠长显著高于0.70%和1.26%Lys组(P0.05)。0.84%Lys组胸腺指数显著高于0.98%、1.12%和1.26%Lys组(P0.05);0.84%、0.98%和1.12%Lys组法氏囊指数显著高于其他组(P0.05)。0.84%、0.98%和1.12%Lys组血清总蛋白和白蛋白含量显著高于0.70%和1.26%Lys组(P0.05);0.98%Lys组血清尿素氮和尿酸含量显著低于1.26%Lys组(P0.05);0.84%Lys组血清碱性磷酸酶活性和血浆生长激素含量均显著高于0.70%Lys组(P0.05)。根据8周龄鸡体重、群体均匀度、全净膛重、屠体重、心脏指数、肝脏指数、胫长、十二指肠重及血清尿酸含量等指标,各自拟合二次曲线,得出最佳饲粮Lys水平分别为0.906%、0.888%、0.931%、0.928%、0.889%、0.902%、0.902%、1.071%和0.958%。综上,推荐5~8周龄京红1号蛋鸡饲粮Lys需要量为0.93%。 相似文献
6.
日粮赖氨酸含量对奶牛产奶性能的影响 总被引：1，自引：1，他引：0
试验选取40头荷斯坦奶牛随机分为4个试验组,每组10头奶牛。试验1组的日粮为14.1%粗蛋白质(CP)和0.60%赖氨酸(Lys),试验2组为14.1%CP和0.45%Lys,试验3组12.6%CP和0.58%Lys及试验4组12.5%CP和0.44%Lys。所有试验奶牛先饲喂10d试验日粮作为预试期,然后开始70d正试期。试验结果表明:试验1组产奶量平均为(22.26±1.50)kg/d,与试验3组([21.98±2.18)kg/d](P0.05);试验1组和3组产奶量均高于试验2组和4组(P0.05);试验2组产奶量([20.83±1.57)kg/d]略高于4组([20.15±1.71)kg/d],差异不显著(P0.05)。因此,奶牛日粮蛋白质从14.1%适当降到12.6%,日粮赖氨酸水平保持在0.60%,奶牛产奶量不受影响,乳蛋白含量有上升趋势;奶牛日粮赖氨酸从0.60%降到0.45%,无论日粮蛋白质水平是否变化,奶牛产奶量下降。 相似文献
7.
苟钟勇林厦菁范秋丽王一冰阮栋张赛叶金玲茅沈丽蒋守群 《动物营养学报》2023,(1):191-202
本试验旨在研究基于玉米-花生仁饼型低粗蛋白质(CP)水平饲粮对1~21日龄黄羽肉鸡生长性能和氮利用率的影响。选取1日龄快速型岭南黄羽肉鸡1920只(公母各占1/2),公、母鸡各按体重一致原则随机分为4组(每组6个重复,每个重复40只)。4个组的饲粮CP水平分别为22.5%、21.5%、20.5%和19.5%。试验期21 d。结果表明:1)饲粮不同水平CP和性别对21日龄肉鸡末重和1~21日龄平均日增重(ADG)均有显著影响(P<0.05)且具有显著交互作用(P<0.05);22.5%CP组和21.5%CP组ADG均显著高于20.5%CP组和19.5%CP组(P<0.05),饲粮CP水平对肉鸡ADG的影响具有显著线性效应(P<0.05)。22.5%CP组公鸡ADG最高,显著高于其他3个CP水平组(P<0.05),21.5%CP组公鸡ADG也显著高于20.5%CP组和19.5%CP组(P<0.05);22.5%CP组和21.5%CP组母鸡ADG均显著高于19.5%CP组(P<0.05)。2)饲粮不同水平CP对肉鸡料重比(F/G)有显著影响(P<0.05)且具有显著的线性效应(P<0.05);22.5%CP组F/G最低,显著低于其他3个水平CP组(P<0.05);21.5%CP组F/G显著低于19.5%CP组(P<0.05)。3)饲粮不同水平CP均对肉鸡氮利用率有显著影响(P<0.05)。21.5%CP组肉鸡氮利用率最高,显著高于其他3个水平CP组(P<0.05),19.5%CP组氮利用率也显著高于20.5%CP组(P<0.05)。由此可见,以玉米-花生仁饼为基础饲粮,低CP水平饲粮中尽管补充添加了晶体氨基酸,但是还是会影响公鸡生长。1~21日龄快速型岭南黄羽肉鸡母鸡饲粮CP水平可以降低1~2个百分点。建议将公、母鸡分开饲养,以实现对其营养的精准调控,提高氮利用率,降低氮排放。 相似文献
8.
本试验旨在研究饲粮粗蛋白质(CP)和外源褪黑激素(MT)水平对水貂生长性能、血清生化指标及营养物质消化率的影响。选取90只50日龄体重一致的健康雄性水貂,随机分为6组,每组15个重复,每个重复1只水貂。采用3×2两因子试验设计,即3个饲粮CP水平(32%、36%和40%)和2个皮下埋植的外源MT水平(0、10 mg/只)。试验期64 d。结果表明:1)40%CP水平时,10 mg/只MT组水貂末重显著高于0 mg/只MT组(P<0.05);32%CP水平时,10 mg/只MT组水貂平均日增重显著高于0 mg/只MT组(P<0.05);饲粮CP和外源MT水平对水貂末重、平均日增重无显著互作效应(P>0.05)。2)32%和40%CP水平时,皮下埋植10mg/只MT可极显著降低水貂血清白蛋白(ALB)含量(P<0.01);32%CP水平的血清甘油三酯(TG)含量均显著高于40%CP水平(P<0.05);饲粮CP和外源MT水平对水貂血清ALB含量存在极显著互作效应(P<0.01),对血清总蛋白(TP)、尿素氮(UN)、TG含量和碱性磷酸酶(ALP)活性无显著互作效应(P>0.05)。3)10 mg/只MT组中,32%和36%CP水平的水貂干物质采食量极显著高于40%CP水平(P<0.01);0 mg/只MT组水貂中,32%CP水平的干物质采食量和干物质排出量极显著高于40%CP水平(P<0.01);饲粮CP和外源MT水平对水貂采食量、干物质采食量、干物质排出量和干物质消化率均无显著互作效应(P>0.05)。4)10 mg/只MT组中,36%CP水平的水貂粗脂肪消化率显著高于32%和40%CP水平(P<0.05),32%CP水平的水貂钙消化率极显著高于40%CP水平(P<0.01),32%CP水平的水貂磷消化率极显著高于36%和40%CP水平(P<0.01);0 mg/只MT组中,36%CP水平钙消化率极显著高于40%CP水平(P<0.01),32%CP水平的水貂磷消化率极显著高于36%和40%CP水平(P<0.01);饲粮CP和外源MT水平对水貂CP、粗脂肪、钙和磷的消化率无显著互作效应(P>0.05)。综合本试验各项测定指标可以得出,皮下埋植10 mg/只外源MT时,水貂较适宜的饲粮CP水平为32%～36%。 相似文献
9.
《畜牧兽医学报》2017,(12)
旨在研究氨基酸平衡低蛋白质日粮对育肥猪肠道抗菌肽与微生物区系的影响。将20头(60±2.5)kg的育肥猪(杜×长×大)随机分为4个处理:14.0%CP日粮(14.0%CP),添加Glu、Lys、Met、Thr和Trp的12.5%CP日粮(12.5%CP),添加Glu、Lys、Met、Thr和Trp的11.0%CP日粮(11.0%CP),添加Glu、Lys、Met、Thr、Trp和BCAA(Val、Ile、Leu)的11.0%CP日粮(11.0%CP+BCAA)。每个处理5个重复,每个重复1头猪。试验分为5d预试期和30d正试期。结果表明:(1)尿液尿素氮和尿酸含量随日粮蛋白质水平降低而显著降低(P0.05);(2)低蛋白质水平日粮显著降低空肠黏膜、回肠黏膜β-defensin-2水平和ANG1、ANG4 mRNA表达水平(P0.05);(3)不同蛋白质水平日粮对部分回肠、直肠食糜微生物氨基酸组成有显著影响(P0.05);(4)与14.0%CP组相比,12.5%CP组Observed species显著提高(P0.05),11.0%CP组Shannon、PD显著降低(P0.05),11.0%CP+BCAA组Shannon显著降低(P0.05)。4组之间拟杆菌门、厚壁菌门的相对丰度差异不显著(P0.05)。与14.0%CP组相比,12.5%CP组和11.0%CP组酸杆菌门、芽单胞菌门相对丰度显著降低(P0.05),11.0%CP+BCAA组芽单胞菌门相对丰度显著降低(P0.05)。14.0%CP组变形菌门相对丰度显著高于12.5%CP组和11.0%CP+BCAA组(P0.05),与11.0%CP组差异不显著(P0.05),14.0%CP组弯曲菌科相对丰度显著高于其他组(P0.05)。14.0%CP组粪肠球菌属、狭义梭菌属的相对丰度显著高于其他组(P0.05),11.0%CP组和11.0%CP+BCAA组毛螺菌属FCS020的相对丰度显著高于12.5%CP组和14.0%CP组(P0.05)。低蛋白质水平氨基酸平衡日粮能提高氮的利用率,影响肠道微生物区系组成,在低蛋白质日粮中添加BCAA有助于增加氨基酸的平衡性、维持肠道免疫机能。 相似文献
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为研究不同水平低粗蛋白质(CP)氨基酸平衡日粮对10～25 kg仔猪的生长性能、血液指标、营养物质表观养分消化率和腹泻率的影响,选取320头10 kg健康的"杜×长×大"三元杂交断奶仔猪,随机分为4个处理,每个处理4个重复,每个重复20头仔猪。对照组饲喂基础日粮,CP水平为20.1%,其他组饲喂低CP补充氨基酸日粮,CP水平分别为18.3%、14.9%、13.2%,除根据需要补充L-赖氨酸、DL-蛋氨酸、L-苏氨酸、L-色氨酸外,还补充L-缬氨酸、L-异亮氨酸、L-苯丙氨酸和L-组氨酸等,共计8种晶体氨基酸,各处理氨基酸水平均达到或超过NRC(1998)猪营养需要量标准,试验期24 d。结果显示:①20.1%CP组仔猪平均日增重(ADG)显著高于13.2%CP(P0.05);各组间料重比(F/G)、平均采食量(ADFI)差异均不显著(P0.05)。②20.1%CP组仔猪血清尿素水平最高,13.2%CP最低,二者差异极显著(P0.01);各组间总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白蛋白/球蛋白、胰岛素、血糖、免疫球蛋白(IgA)、IgG、IgM水平均差异不显著(P0.05)。③13.2%CP、14.9%CP组仔猪CP表观消化率极显著低于20.1%CP组(P0.01);其余指标差异均不显著(P0.05)。④20.1%CP组仔猪腹泻率最高,13.2%CP组腹泻率最低,且差异显著(P0.05)。综上所述,日粮CP水平降低至14.9%时对仔猪的生长性能无不良影响;血清尿素水平、CP表观消化率均极显著对于对照组(20.1%CP),仔猪腹泻率大大降低。因此10～25 kg断奶仔猪氨基酸平衡日粮CP水平可降低至14.9%,不影响仔猪的生长性能。 相似文献
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Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size. 相似文献
13.
In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum. 相似文献
14.
Nadine Pa?lack Barbara Mainzer Monika Lahrssen-Wiederholt Helmut Schafft Richard Palavinskas Angele Breithaupt Jürgen Zentek 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):57-66
This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations. 相似文献
15.
《饲料工业》2019,(18):54-58
应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。 相似文献
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K Reifenberg M Stahl U L?sch 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(3):191-198
A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays. 相似文献
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《The Journal of small animal practice》1975,16(1-12):143-154
Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds. 相似文献