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1.
为明确江西省山药病原线虫的种类及地理分布,对采自主产区的157份山药样品进行了线虫分离与形态学鉴定,并对主要病原线虫种类进行rDNA-ITS和28S的序列比对和种群系统进化分析。形态学和分子鉴定结果表明:山药病原线虫种类为咖啡短体线虫Pratylenchus coffeae和南方根结线虫Meloidogyne incognita。调查结果表明,咖啡短体线虫在江西省大部分山药产区广泛分布,是优势种群。吉安市永丰县和泰和县山药线虫危害最为严重,山药样品中线虫检出率分别为66.7%和88.9%。南方根结线虫在江西省山药产区不是优势种群,呈零星发生。此外,山药样品中存在两种线虫的单一种群侵染和复合种群侵染,分别占总样品数的87.1%和12.9%。综上,本研究明确了江西省山药主产区病原线虫的优势种是咖啡短体线虫,为江西省山药病原线虫的科学防控提供了有价值的参考依据。  相似文献   

2.
运用比较形态学、同工酶和mtDNA-PCR对采自海南岛4个市县的葫芦科蔬菜根结线虫进行鉴定,结果发现5个种群均存在象耳豆根结线虫(Meloidogyne enterolobii)。该种线虫是葫芦[Lagenaria siceraria (Molina) Standl]和丝瓜[Luf〖KG-0.5mm〗f〖KG-0.1mm〗a cylindrica (Linn.) Roem]上的新记录种,文昌和陵水属其首次被报道的分布地区。有2个样本表现为象耳豆根结线虫和南方根结线虫(M. incognita)的混合种群。  相似文献   

3.
新疆设施蔬菜根结线虫病发生调查及病原鉴定   总被引:1,自引:0,他引:1  
对新疆蔬菜根结线虫病的发生情况进行了调查,发现蔬菜根结线虫在和田、阿克苏、库尔勒、吐鲁番、伊犁等南北疆设施蔬菜上均有分布和发生危害,露地蔬菜尚未见发生。从各发生地温室中采集14份蔬菜根结线虫样本,寄主主要是番茄,另外还有豇豆、黄瓜、生菜、香芹、苦瓜。采用形态学以及根结线虫特异性引物PCR相结合的方法进行鉴定,结果表明新疆设施蔬菜上有南方根结线虫(Meloidogyne incognita)和北方根结线虫(M.hapla)两种,优势种群为南方根结线虫。  相似文献   

4.
云南省灯盏花根际土壤寄生线虫的种类鉴定   总被引:1,自引:1,他引:0  
2001-2005年对云南省灯盏花主要栽培区的灯盏花根部及根际土壤的寄生线虫进行分类鉴定,共鉴定出9种线虫:内卷滑刃线虫(Aphelenchoides involutus)、燕麦真滑刃线虫(Aphelenchus avenae)、异头丝尾垫刃线虫(Filenchus heterocephalus)、双宫螺旋线虫(Helicotylenchus dihystera)、南方根结线虫(Meloidogyne incognita)、草地短体线虫(Pratylenchus pratensis)、活泼平滑垫刃线虫(Psilenchus hilarulus)、戴维恩垫刃线虫(Tylenchusdavainei)和广东垫刃线虫(Tylenchus guangdongensis)。优势种群是双宫螺旋线虫,其次是南方根结线虫。灯盏花是这些线虫的新记录寄主。  相似文献   

5.
红麻根结线虫生活习性及防治研究   总被引:1,自引:1,他引:1  
研究表明,我国红麻上的根结线虫有南方根结线虫(Meloidogyne incognita)1号和2号小种,爪哇根结线虫(M.javanica)和花生根结线虫(M.arenaria)2号小种,南方根结线虫是优势种。它们都不为害棉花、花生、小麦、芝麻和苎麻,对高粱和玉米为害极轻。丝瓜、西瓜、番茄和空心莲子草为其共同寄主。南方根结线虫还侵染大豆、豇豆、苦瓜、菜瓜、南瓜、茄子、辣椒、烟草、黄麻、甘薯、黄瓜、白菜、莴苣、菜豆和扁豆等作物。红麻根结线虫的二龄幼虫主要分布在0~40cm 土层内,砂壤土中的线虫密度大于黄壤土。病原线虫以卵和二龄幼虫主要在病株残体中越冬。实行轮作、清除病残和药剂处理土壤是防治本病的有效措施。  相似文献   

6.
福建省花生根部寄生线虫种类鉴定   总被引:2,自引:0,他引:2  
从福建省花生(Arachis hypogaea Linn.)根部及根际土壤中共分离鉴定出11种植物寄生线虫:肾状肾形线虫(Rotylenchulus reniformis)、咖啡短体线虫(Pratylenchus coffeae)、饰环矮化线虫(Tylenchorhynchus annula-tus)、装饰小环线虫(Criconemella ornata)、双宫螺旋线虫(Helicotylenchus dihystera)、食菌茎线虫(Ditylenchusmyceliophagus)、蘑菇滑刃线虫(Aphlenchoides composticola)、燕麦真滑刃线虫(Aphelenchus avenae)、广东杆垫刃线虫(Rhabdotylenchus guangdongensis)、丝尾垫刃线虫(Filenchus sp.)和根结线虫(Meloidogyne sp.)。其中肾状肾形线虫、咖啡短体线虫、装饰小环线虫和根结线虫为花生的重要寄生线虫。  相似文献   

7.
巴拉那根结线虫(Meloidogyne paranaensis)是咖啡上最具有毁灭性的根结线虫之一,可造成咖啡减产高达50%,目前主要发生在巴西、危地马拉和墨西哥,我国没有分布。本文介绍了巴拉那根结线虫的形态鉴定特征和生物学特性等内容。  相似文献   

8.
南方根结线虫中国分离群体种内变异分析   总被引:1,自引:0,他引:1  
为调查我国不同地区和不同寄主上的根结线虫Meloidogyne spp.种类分布以及群体变异情况,基于酯酶和苹果酸脱氢酶同工酶图谱及SCAR分子标记技术对2017—2019年从6省19种植物根部组织分离到的40个根结线虫群体进行鉴定,针对南方根结线虫M.incognita群体分别通过寄主鉴别法进行生理小种鉴别,利用携带Mi抗性基因的番茄进行毒力测试,对2龄幼虫的口针长度和体长进行测量,并对核糖体ITS和线粒体Nad5基因序列进行比较分析。结果显示:根结线虫分离群体经鉴定包括38个南方根结线虫群体和2个象耳豆根结线虫M.erterolobii群体;38个南方根结线虫群体中有35个群体被鉴别为1号生理小种,其余3个群体被鉴别为2号生理小种;发现1个南方根结线虫群体CN19可在携带Mi抗性基因的番茄上侵染繁殖,为毒性群体,其余群体无法进行侵染和繁殖,为无毒群体。南方根结线虫群体2龄幼虫的口针长度和体长均差异较大,而不同寄主来源分离群体的ITS和Nad5基因序列也存在一定变异。基于ITS和Nad5基因序列构建的系统发育树将所有根结线虫群体归为南方根结线虫和象耳豆根结线虫组成的2个独立分支,但不能确定南方根结线虫不同群体的分子进化与其寄主来源和地理分布之间的相关性。  相似文献   

9.
1997-1998年,对南京口岸进口的荷兰、加拿大、美国、韩国、台湾和香港等国家和地区41批(次)园林植物检疫中,1次截获鳞球茎类线虫、19交截获咖啡短体线虫、草莓芽叶线虫、克鲁克剑线虫、毛刺尾线虫、南方根结线虫、花生根结线虫、北方根结线虫和其它根结线虫等,进口的园林植物中危险性疫情发生率高达34%。对江苏5个主要出口园林植物生长基地进行的植物寄生线虫种类调查,在59种经常性出口的园林植物中,发现27属植物寄生线虫,其中已鉴定出13种。对红花木莲苗上的南方根结线虫、牡丹苗上的腐烂茎线虫进行的检疫处理试验表明,用几种农药混配的处理液加热后处理植物的根部,可以有效地杀死这两种植物寄生线虫,保证货物安全出口。  相似文献   

10.
运用比较形态学、同工酶和 mtDNA-PCR 对采自海南岛9个市县的南药根结线虫进行种类鉴定,结果发现有南方根结线虫、象耳豆根结线虫、爪哇根结线虫、花生根结线虫和西班牙根结线虫等5种根结线虫危害海南岛南药。5种根结线虫均能在海巴戟上寄生与危害,而南方根结线虫寄主非常广泛,可危害大多数调查的药用植物。  相似文献   

11.
Fifteen species of dermestid beetles were recorded at ‘Evolution Canyon’ (EC), Lower Nahal Oren, Mt. Carmel, Israel. They represent ~35% of known Israeli dermestid species. The following three species were recorded for the first time in Israel:Trogoderma svriaca Dalla Torre, 1911;Ctesias svriaca Ganglbauer, 1904; andAnthrenus (s.str.) jordaniens Pic, 1934. Adults of 13 species were collected on the more solar radiated, warmer and climatically more fluctuating south-facing slope (SFS); ten species were collected on the opposite, north-facing slope (NFS), which was cooler and climatically more stable. The abundance of adult dermestid beetles was 1.9 times higher on the SFS than on the NFS (86 and 47, respectively). Species richness and abundance distribution at EC (three collecting stations on each slope and one at the valley bottom) were significantly negatively correlated with the plant cover that consisted of trees and bushes (Spearmanr s ,P=0.007 and 0.039, respectively) and perennials (Spearmanr s ,P=0.039 and 0.077, respectively), indicating that non-woody plants were preferred by adult dermestid beetles.  相似文献   

12.
We first discuss the diversity of fruit fly (Diptera: Tephritidae) parasitoids (Hymenoptera) of the Neotropics. Even though the emphasis is on Anastrepha parasitoids, we also review all the information available on parasitoids attacking flies in the genera Ceratitis, Rhagoletis, Rhagoletotrypeta, Toxotrypana and Zonosemata. We center our analysis in parasitoid guilds, parasitoid assemblage size and fly host profiles. We also discuss distribution patterns and the taxonomic status of all known Anastrepha parasitoids. We follow by providing a historical overview of biological control of pestiferous tephritids in Latin American and Florida (U.S.A.) and by analyzing the success or failure of classical and augmentative biological control programs implemented to date in these regions. We also discuss the lack of success of introductions of exotic fruit fly parasitoids in various Latin American countries. We finish by discussing the most pressing needs related to fruit fly biological control (classical, augmentative, and conservation modalities) in areas of the Neotropics where fruit fly populations severely restrict the development of commercial fruit growing. We also address the need for much more intensive research on the bioecology of native fruit fly parasitoids.  相似文献   

13.
Molecular diagnostic techniques have been developed to differentiate the Ascochyta pathogens that infect cool season food and feed legumes, as well as to improve the sensitivity of detecting latent infection in plant tissues. A seed sampling technique was developed to detect a 1% level of infection by Ascochyta rabiei in commercial chickpea seed. The Ascochyta pathogens were shown to be genetically diverse in countries where the pathogen and host have coexisted for a long time. However, where the pathogen was recently introduced, such as A. rabiei to Australia, the level of diversity remained relatively low, even as the pathogen spread to all chickpea-growing areas. Pathogenic variability of A. rabiei and Ascochyta pinodes pathogens in chickpea and field pea respectively, appears to be quantitative, where measures of disease severity were based on aggressiveness (quantitative level of infection) rather than on true qualitative virulence. In contrast, qualitative differences in pathogenicity in lentil and faba bean genotypes indicated the existence of pathotypes of Ascochyta lentis and Ascochyta fabae. Therefore, reports of pathotype discrimination based on quantitative differences in pathogenicity in a set of specific genotypes is questionable for several of the ascochyta-legume pathosystems such as A. rabiei and A. pinodes. This is not surprising since host resistance to these pathogens has been reported to be mainly quantitative, making it difficult for the pathogen to overcome specific resistance genes and form pathotypes. For robust pathogenicity assessment, there needs to be consistency in selection of differential host genotypes, screening conditions and disease evaluation techniques for each of the Ascochyta sp. in legume-growing countries throughout the world. Nevertheless, knowledge of pathotype diversity and aggressiveness within populations is important in the selection of resistant genotypes.  相似文献   

14.
Recent data on the epidemiology of the common mycotoxigenic species of Fusarium, Alternaria, Aspergillus and Penicillium in infected or colonized plants, and in stored or processed plant products from the Mediterranean area are reviewed. Emphasis is placed on the toxigenicity of the causal fungal species and the natural occurrence of well known mycotoxins (aflatoxins, ochratoxins, fumonisins, trichothecenes, zearalenone, patulin, Alternaria-toxins and moniliformin), as well as some more recently described compounds (fusaproliferin, beauvericin) whose toxigenic potential is not yet well understood. Several Fusarium species reported from throughout the Mediterranean area are responsible of the formation of mycotoxins in infected plants and in plant products, including: Fusarium graminearum, F. culmorum, F. cerealis, F. avenaceum, F. sporotrichioides and F. poae, which produce deoxynivalenol, nivalenol, fusarenone, zearalenone, moniliformin, and T-2 toxin derivatives in wheat and other small grains affected by head blight or scab, and in maize affected by red ear rot. Moreover, strains of F. verticillioides, F. proliferatum, and F. subglutinans, that form fumonisins, beauvericin, fusaproliferin, and moniliformin, are commonly associated with maize affected by ear rot. Fumonisins, were also associated with Fusarium crown and root rot of asparagus and Fusarium endosepsis of figs, caused primarily by F. proliferatum. Toxigenic A. alternata strains and associated tenuazonic acid and alternariols were commonly found in black mould of tomato, black rot of olive and citrus, black point of small cereals, and black mould of several vegetables. Toxigenic strains of A. carbonarius and ochratoxin A were often found associated with black rot of grapes, whereas toxigenic strains of A. flavus and/or P. verrucosum, forming aflatoxins and ochratoxin A, respectively, were found in moulded plant products from small cereals, peanuts, figs, pea, oilseed rape, sunflower seeds, sesame seeds, pistachios, and almonds. Finally, toxigenic strains of P. expansum and patulin were frequently found in apple, pear and other fresh fruits affected by blue mould rot, as well as in derived juices and jams.  相似文献   

15.
《干旱区科学》2014,(6):782-782
正Journal of Arid Land(JAL)is an international journal(ISSN 1674-6767;CN 65-1278/K)for the natural sciences,sponsored by the Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences and Science Press.It is published by Science Press and Springer-Verlag Berlin Heidelberg bimonthly.JAL publishes original,innovative,and integrative research from arid and semiarid regions,ad  相似文献   

16.
The genera ofMicrogaster Latreille 1804 andHygroplitis Thomson 1895 from China are presented systematically in this paper. Thirty-two species ofMicrogaster and three species ofHygroplitis are known in China. Diagnosis, character variation, distribution and host of each species among the two genera are presented, including its host and distribution. Keys to the species ofMicrogaster andHygroplitis are given. http://www.phytoparasitica.org posting Dec. 19, 2006.  相似文献   

17.
Plant Viruses Transmitted by Whiteflies   总被引:18,自引:0,他引:18  
One-hundred and fourteen virus species are transmitted by whiteflies (family Aleyrodidae). Bemisia tabaci transmits 111 of these species while Trialeurodes vaporariorum and T. abutilonia transmit three species each. B. tabaci and T. vaporariorum are present in the European–Mediterranean region, though the former is restricted in its distribution. Of the whitefly-transmitted virus species, 90% belong to the Begomovirus genus, 6% to the Crinivirus genus and the remaining 4% are in the Closterovirus, Ipomovirus or Carlavirus genera. Other named, whitefly-transmitted viruses that have not yet been ranked as species are also documented. The names, abbreviations and synonyms of the whitefly-transmitted viruses are presented in tabulated form together with details of their whitefly vectors, natural hosts and distribution. Entries are also annotated with references. Whitefly-transmitted viruses affecting plants in the European–Mediterranean region have been highlighted in the text.  相似文献   

18.
Liriomyza cicerina (Diptera: Agromyzidae) is an important pest on chickpea in Turkey. The objective of this study was to determine the parasitoids and rates of parasitism ofL. cicerina on chickpea (Cicer arietinum L.) during the 2005 and 2006 seasons in ?anl?urfa province, Turkey. Leaves with mines were sampled weekly and kept in the laboratory to observe and count emerging leafminer and parasitoid adults. Eight parasitoid species were collected: the braconidsOpius monilicornis Fischer andOpius tersus Foerster and the eulophidsDiaulinopsis arenaria (Erdös) andNeochrysocharis formosa (Westwood), which occurred in both the winter and summer seasons;Diglyphus crassinervis Erdös,Neochrysocharis ambitiosa Hansson,Neochrysocharis sericea (Erdös) andPediobius metallicus (Nees), which occurred only in the summer growing areas.Diaulinopsis arenaria was the predominant parasitoid with 4–7.7% parasitism rate whileN. ambitiosa andO. monilicornis were the second and third most predominant species. The results of these trials show that sinceDia. arenaria occurred throughout every season, it could potentially be used for control of the leafminerL. cicerina.  相似文献   

19.
Systematic information on the quantitative impact of Z ygogramma bicolorata on the biology of P arthenium hysterophorus is crucial as the seeds of this weed continue to germinate from the accumulated soil seed bank throughout the year in the form of different germinating flushes, while the activity of the beetle ceases during winter as it enters diapause. Therefore, plant–herbivore interactions need to be explored to develop predictions of the overall impact of the introduced beetle on the weed. The findings revealed that defoliation by Z . bicolorata had a significant impact on the plant height, density and flower production in flushes F 3, F 4 and F 5, but not in F 1 and F 2 that exhibited longer periodicity, profuse branching, a longer flowering period and maximum flower production and contributed mostly to the existing seed soil bank. Therefore, total depletion of the existing soil seed bank was not possible. Consequently, the effect of augmentative field releases of laboratory‐reared beetles was explored on F 1 and F 2 in February for three consecutive years (2011–2013). Before initiating the trial, random soil samples were taken from the plots that were assigned to the paired treatments (i.e. with the beetle and without the beetle [insecticide‐treated]) and it was found that the seed bank in those samples did not differ. The single release of Z . bicolorata adults at five per plant at the six‐leaf stage significantly reduced the soil seed bank, compared to without the biocontrol agent, irrespective of the flushes at the end of the season.  相似文献   

20.
Solacol®, a formulation of the antibiotic validamycin, at 0.33% in 2% malt extract agar, reduced the spread of fungi on dilution plates drastically and allowed twice as much incubation time before subculturing; this resulted in an elevated number of species isolated. Using pure cultures of 62 common soil fungi, it was shown that all fast-growing species (exceptPythium ultimum) were efficiently inhibited but not completely suppressed. Inhibition was comparable to that by 0.5% oxgall, though, while this substance completely suppressed several species, Solacol very strongly inhibited onlyGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum andRhizoctonia solani. In a further experiment each separate constituent of Solacol was tested against 22 fungi at equivalent concentrations. Validamycin strongly inhibitedChaetomium globosum and two Basidiomycetes, though hardly more than the non-ionic detergent which mainly inhibited the other fungi. A few species were, however, more inhibited by Solacol than by the detergent alone. Solacol at 0.33% is a suitable aid in dilution plating of soil fungi, by increasing the number of colonies and species observed.Samenvatting Solacol®, een formulering van het antibioticum validamycine, remde de groei van schimmels in verdunningsplaten met een concentratie van 0.33% in 2% moutagar en maakte het mogelijk de periode tot afenten met een factor 2 te verlengen; daardoor was het aantal geïsoleerde soorten duidelijk toegenomen. Met reincultures van 62 algemene grondschimmelsoorten werd aangetoond, dat alle snelgroeiende soorten (met uitzondering vanPythium ultimum) voldoende geremd, maar niet volkomen onderdrukt werden. Het remmingspercentage was vergelijkbaar met dat van 0.5% ossegal, hoewel dit laatste sommige soorten volkomen onderdrukte; Solacol remde alleenGaeumannomyces graminis, Gerlachia nivalis, Harzia acremonioides, Verticillium biguttatum enRhizoctonia solani zeer sterk. In een volgend experiment werden de componenten van Solacol t.o.v. 22 fungi apart getoetst in concentraties equivalent aan 0.33% Solacol. Validamycine remde alleenChaetomium globosum en twee basidiomyceten behoorlijk, maar nauwelijks meer dan de niet-ionische uitvloeier, die in hoofdzaak de overige remeffecten veroorzaakte. Enkele soorten werden echter door het complete Solacol veel sterker geremd dan door de uitvloeier alleen. Solacol in een verdunning van 0,33% wordt aanbevolen bij verdunningsplaten voor het isoleren van grondschimmels ten einde het aantal kolonies en soorten te verhogen.  相似文献   

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