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1.
以黑籽南瓜为砧木,以津绿21-1和津研4号2个黄瓜品种为接穗,对嫁接植株的光合指标及叶片激素含量和可溶性蛋白进行对比研究.结果表明,2个品种嫁接植株的净光合速率(Pn)、气孔导度(Gs)和胞间CO2浓度(Ci)均极显著高于自根植株;嫁接植株叶片GA和ABA含量也与自根植株存在显著差异,嫁接植株叶片ABA/GA值在测定的各个时期均显著高于自根植株;通过等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)对叶片可溶性蛋白进行分析对比,发现在嫁接植株叶片中产生了1~2种特异蛋白.  相似文献   

2.
以黑籽南瓜(Cucurbita ficifolia Bouché)、日本南瓜(Cucurbita moschata)、'甬砧8号'白籽南瓜(Cucurbita maxima×Cucurbita moschata'Yongzhen No.8')、'五叶香'丝瓜(Luffa cylindrica'Wuyexiang')和'傲美'苦瓜(Momordica charantin'Aomei')为砧木,以'春秋王2号'黄瓜(Cucumis sativus'Chunqiuwang No.2')为接穗,研究了大棚自然高温对不同砧木黄瓜嫁接植株生理特性及果实品质的影响。结果表明:高温下嫁接植株和自根植株叶片中丙二醛和脯氨酸含量升高,根系活力下降,且嫁接植株叶片中丙二醛含量低于自根植株,脯氨酸含量和根系活力高于自根植株;不同砧木嫁接植株果实的可溶性蛋白含量基本无差异,果实可溶性糖含量、游离氨基酸含量及VC含量则表现为嫁接植株高于自根植株,其中以'五叶香'丝瓜砧木嫁接植株的果实品质为最佳。  相似文献   

3.
嫁接对薄皮甜瓜光合特性、伤流液激素和产量的影响   总被引:1,自引:0,他引:1  
试验选用玉美人薄皮甜瓜(Cucumis melo L.)做接穗,以圣砧1号白子南瓜为砧木进行嫁接,以自根苗为对照,研究嫁接对薄皮甜瓜的光合特性、伤流液中激素和产量的影响.结果表明,嫁接明显地增加了薄皮甜瓜的总叶面积,其茎蔓生长明显快于自根植株.各个时期嫁接植株的各器官的干物重均高于自根植株.但嫁接对薄皮甜瓜叶片中叶绿素的含量影响不大,并且对植株生长发育各时期的功能叶片的净光合速率、蒸腾速率、气孔导度及胞间CO2浓度影响不明显.因此,嫁接主要是通过扩大植株的总叶面积来提高植株的光合速率的.伤流液中玉米素(ZT)、赤霉素(GAs)和脱落酸(ABA)的浓度均低于自根植株,但ZT和GAs的含量高于自根苗,而ABA的含量低于自根苗.嫁接植株的增产效果显著,其平均单瓜重和公顷产量均高于自根植株.  相似文献   

4.
以日本引进的耐盐品种帝王新土佐南瓜为砧木、京欣二号西瓜品种为接穗,在NaCl胁迫下对营养液栽培嫁接植株与自根植株的生长动态及叶片生理生化特性进行了比较研究。结果表明.嫁接植株在生长前期各形态指标与自根植株差异不明显,但在生长的中期和后期各形态指标均显著高于自根植株;嫁接植株叶片的净光合速率(R)、气孔导度(Gs)和胞间CO2浓度(Ci)均显著高于自根植株;嫁接植株叶片超氧化物歧化酶(SOD)活性、过氧化物酶(tK)D)活性、脯氨酸含量及根系活力均显著高于自根植株,而丙二醛(MDA)含量显著低于自根植株。以上结果表明.在所测定的指标方面西瓜嫁接植株的耐盐性显著强于自根植株。  相似文献   

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以黑籽南瓜(Cucurbita ficifolia ficifolia Bouche)、日本南瓜(Cucurbita moschata)、‘甬砧8号'白籽南瓜(Cucurbita maxima×Cucurbita moschata‘Yongzhen No.8')、‘五叶香'丝瓜(Luffa cylindrica‘Wuyexiang')和‘傲美'苦瓜(Momordica charantin‘Aomei')为砧木,‘春秋王2号'黄瓜(Cucumis sativus‘Chunqiuwang No.2')为接穗,采用营养液栽培法,研究了不同砧木黄瓜嫁接苗的成活率,以及开花结果期嫁接植株和自根植株在25℃(CK)和35℃根际温度下处理5 d及恢复生长5 d后的生理特性及果实品质。结果表明:黑籽南瓜砧木嫁接苗成活率最高;根际高温处理后,开花结果期的嫁接植株和自根植株叶片丙二醛、脯氨酸、可溶性糖含量及抗氧化酶活性升高,根系活力下降;可溶性蛋白含量在‘傲美'苦瓜砧、黑籽南瓜砧嫁接植株和自根植株叶片内降低,在其他砧木嫁接植株叶片内升高;日本南瓜砧木嫁接植株果实可溶性糖和可溶性蛋白含量最高,游离氨基酸含量仅次于黑籽南瓜砧木嫁接植株果实,VC含量仅次于‘五叶香'丝瓜砧木嫁接植株果实。综合来看,日本南瓜作砧木嫁接植株果实品质最佳。研究还表明:在开花结果期不同砧木黄瓜嫁接植株对根际高温的耐受性存在明显差异,其中以‘五叶香'丝瓜作砧木的嫁接植株耐受性最强。  相似文献   

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为探讨外源ABA调控寒地冬小麦耐受低温胁迫的生理生化机制,试验分别用0、0.1、1、10、100μmol.L-1的ABA对强抗寒冬小麦品种"东农冬麦1号"和弱抗寒品种"济麦22"的三叶期麦苗进行根际浇灌。田间自然降温条件下,分别于日最低温度达0、-10和-25℃时取叶片和分蘖节测定生理生化指标,调查麦苗翌年返青率。结果表明,100μmol.L-1ABA处理显著降低两种小麦叶片和分蘖节中渗透调节物质含量、ABA含量及ABA/GA比值,对东农冬麦1号叶片和分蘖节及济麦22分蘖节GA含量无显著影响,但济麦22叶片中GA含量增加。两品种小麦MDA含量减少,SOD活性显著增加,细胞膜透性增大。10μmol.L-1ABA处理后东农冬麦1号叶片和分蘖节中渗透调节物质含量、ABA含量及ABA/GA比值显著增加,GA含量和MDA含量减少,SOD活性增强。济麦22叶片和分蘖节中可溶性蛋白含量及膜脂过氧化无显著影响,但其叶片和分蘖节可溶性糖含量及分蘖节中ABA含量、ABA/GA比值降低。其他浓度对两种小麦膜脂过氧化及可溶性蛋白含量无显著影响。可见,10μmol.L-1ABA可促进东农冬麦1号抗寒性的提高,而对济麦22效果不显著。  相似文献   

7.
[目的]研究赤霉素对青花菜叶片生理特性的影响。[方法]以青花菜品种"早生绿"为材料,以清水处理为对照(CK),用不同浓度(50、100、150 mg/L)赤霉素(GA)喷施叶面,研究GA处理对青花菜叶片可溶性糖、可溶性蛋白质、游离氨基酸含量和酸性转化酶活性的影响。[结果]在花序分化期,各GA处理青花菜叶片中可溶性糖、可溶性蛋白含量和酸性转化酶活性均高于CK,处理间差异不显著;100 mg/L GA处理的叶片游离氨基酸含量高于CK,其余2个处理均低于CK。在现蕾期,50、100 mg/L GA处理的叶片可溶性糖含量显著高于CK;100、150 mg/L GA处理的叶片可溶性蛋白含量和酸性转化酶活性显著高于CK;GA各处理叶片游离氨基酸含量均低于CK;[结论]GA处理可增加青花菜叶片可溶性糖和可溶性蛋白的含量。  相似文献   

8.
不同砧木嫁接黄瓜果实感官评价与营养品质的相关性   总被引:1,自引:0,他引:1  
以不同中国南瓜品种为砧木的嫁接黄瓜和自根黄瓜为试验材料,对其果实的感官品质指标和营养指标进行相关性分析和逐步回归分析,得出嫁接黄瓜的可溶性糖、游离氨基酸含量均高于自根黄瓜。其中,维生素C(x1)、可溶性糖(x2)、可溶性蛋白、游离氨基酸(x3)含量与感官评价呈正相关,而可滴定酸含量(x4)与感官评价呈负相关,可利用回归方程y=0.062x1+3.843x2+0.400x3-9.680x4-3.576对嫁接黄瓜的感官品质做出初步评价。  相似文献   

9.
以不结球白菜感病品种‘矮脚黄’为材料,采用ELISA和Real-time PCR技术研究了芜菁花叶病毒(TuMV)侵染后不结球白菜内源生长素(IAA)、赤霉素(GA3)、脱落酸(ABA)和茉莉酸(JA)的动态变化以及内源激素相关基因的表达差异。结果表明:TuMV侵染后,植株发病症状的严重程度与IAA含量呈正相关;光敏色素相关蛋白基因PAP1对IAA的合成起负调控作用。处理植株体内GA3含量及其代谢相关基因,即原表皮因子基因PDF1表达量均明显低于对照植株,而ABA含量则随着接种时间的延长不断积累,始终高于对照植株。且发病症状越严重的植株中,GA3含量越低,ABA含量越高。处理植株JA含量和JA诱导基因,即营养贮藏蛋白基因Vsp1表达量在接种后均出现2次上升,且植株病症越严重,JA含量越低。TuMV侵染后,植株中IAA/ABA和GA3/ABA的值则明显低于对照植株,表明TuMV的侵染破坏了内源激素的平衡,干扰了植物的正常生长发育。  相似文献   

10.
ABA和JA等激素参与葡萄对低温胁迫的应答   总被引:2,自引:0,他引:2  
以抗寒性不同的2个葡萄品种(贝达和梅鹿辄)一年生枝条叶片为材料,研究不同温度(25℃、5℃、-15℃)下葡萄叶片中IAA、GA、iPA、ABA和JA几种内源激素含量及比值的变化。结果表明,常温条件下(25℃),抗寒性强的贝达叶片中ABA、JA和iPA含量均高于抗寒性弱的梅鹿辄,而IAA、GA含量则低于梅鹿辄;低温(5℃、-15℃)下,2个葡萄品种叶片中均出现ABA和JA的猝发,而IAA、GA、iPA则显示出先降低后有所升高的变化;其中ABA和JA分别与IAA和GA的比值随品种抗寒性的增强及温度的降低而显著增大,与品种抗寒性呈负相关。  相似文献   

11.
Anatomical and physiological observations in monkeys indicate that the primate visual system consists of several separate and independent subdivisions that analyze different aspects of the same retinal image: cells in cortical visual areas 1 and 2 and higher visual areas are segregated into three interdigitating subdivisions that differ in their selectivity for color, stereopsis, movement, and orientation. The pathways selective for form and color seem to be derived mainly from the parvocellular geniculate subdivisions, the depth- and movement-selective components from the magnocellular. At lower levels, in the retina and in the geniculate, cells in these two subdivisions differ in their color selectivity, contrast sensitivity, temporal properties, and spatial resolution. These major differences in the properties of cells at lower levels in each of the subdivisions led to the prediction that different visual functions, such as color, depth, movement, and form perception, should exhibit corresponding differences. Human perceptual experiments are remarkably consistent with these predictions. Moreover, perceptual experiments can be designed to ask which subdivisions of the system are responsible for particular visual abilities, such as figure/ground discrimination or perception of depth from perspective or relative movement--functions that might be difficult to deduce from single-cell response properties.  相似文献   

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The aim of this paper is to make possible dialogue between those who claim that technologies are coded with social, political, or ethical values and those who argue that they are value-neutral. To demonstrate the relevance of this bridge-building project, the controversy regarding agrifood biotechnology will be used as a case study. Drawing on work by L. H. Nelson about the nature of human knowledge-building enterprises and E. F. Kittay’s account of the relationally-constituted self, the argument will be made that all technologies embody the values of the communities that created them. Zahra Meghani is an assistant professor in the Department of Philosophy at the University of Rhode Island. Her research interests are philosophy of technology, feminist theory (especially feminist epistemology and feminist philosophy of science), normative ethics, practical ethics (especially health care ethics), disability issues, and political theory.  相似文献   

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The primary rocks are a sequence of titanium-rich basic volcanics, composed of clinopyroxene, plagioclase, and ilmenite with minor olivine, troilite, and native iron. The soil and microbreccias are respectively loose and compacted mixtures of fragments and aggregates of similar rocks, minerals, and glassy fragments and spheres. Impact events are reflected by the presence of shock metamorphosed rock fragments, breccias, and glasses and their resulting compaction to form complex breccias, glass-spattered surfaces, and numerous glass-lined craters. Chemistry of the glasses formed by the impact events is highly variable, and the high iron and nickel content of a few moundlike features suggests that at least some of the projectiles are iron and nickel-rich meteorites.  相似文献   

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R & D, through its effects on the rate of productivity increase, can significantly restrain the rate of inflation in the medium and long run. High rates of inflation damage the workings of the price system and impair the efficiency of practically all economic activities, including R & D. Findings suggest that the percentage increase between 1969 and 1979, in total real R & D expenditures, has been exaggerated due to the inadequacy of the gross national product deflator as applied to R & D.  相似文献   

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Maslansky R 《Science (New York, N.Y.)》2003,301(5639):1479-82; author reply 1479-82
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