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1.
Abstract

We studied the effects of the application of organic matter (OM) and chemical fertilizer (CF) on soil alkaline phosphatase (ALP) activity and ALP-harboring bacterial communities in the rhizosphere and bulk soil in an experimental lettuce field in Hokkaido, Japan. The ALP activity was higher in soils with OM than in soils with CF, and activity was higher in the rhizosphere for OM than in the bulk soil. Biomass P and available P in the soil were positively related to the ALP activity of the soil. As a result, the P concentration of lettuce was higher in OM soil than in CF soil. We analyzed the ALP-harboring bacterial communities using polymerase chain reaction based denaturing gradient gel electrophoresis (DGGE) on the ALP genes. Numerous ALP genes were detected in the DGGE profile, regardless of sampling time, fertilizer treatment or sampled soil area, which indicated a large diversity in ALP-harboring bacteria in the soil. Several ALP gene fragments were closely related to the ALP genes of Mesorhizobium loti and Pseudomonas fluorescens. The community structures of the ALP-harboring bacteria were assessed using principal component analysis of the DGGE profiles. Fertilizer treatment and sampled soil area significantly affected the community structures of ALP-harboring bacteria. As the DGGE bands contributing to the principal component were different from sampling time, it is suggested that the major bacteria harboring the ALP gene shifted. Furthermore, there was, in part, a significant correlation between ALP activity and the community structure of the ALP-harboring bacteria. These results raise the possibility that different ALP-harboring bacteria release different amounts and/or activity of ALP, and that the structure of ALP-harboring bacterial communities may play a major role in determining overall soil ALP activity.  相似文献   

2.
Proteolytic bacterial communities, which mineralize organic nitrogen, play a key role in agricultural systems. In this study, alkaline metalloprotease (apr) gene fragments from proteolytic bacteria were investigated in bulk and rhizosphere paddy soil from four fields under organic management (for 2, 3, 5, and 9 years), and from one field under conventional management (for 2 years). We analyzed the abundance and structure of the proteolytic bacterial communities using real-time quantitative PCR and denaturing gradient gel electrophoresis. Our results showed that the abundance of proteolytic bacteria ranged from 1.57?×?108 to 8.02?×?108?copies/g of soil. In addition, the abundance of the proteolytic bacteria in the paddy soils under organic management was significantly higher than those in the paddy soil under conventional management. Moreover, the gene copy numbers in the rhizosphere soils were significantly higher than those in the bulk soils. The abundance of proteolytic bacteria tended to increase with the duration of organic management, with the highest abundance being found in the soil that had been under organic management for 5 years. However, the proteolytic bacteria communities in the paddy soils were not significantly affected by management practices. Phylogeny analysis showed that all gel bands obtained represented genes from Pseudomonas. Additionally, correlation analysis and canonical correspondence analysis showed that C/N, C, and N were important factors that influenced the abundance and community structure of the proteolytic bacteria. These results suggest that proteolytic bacteria are indicators in organic management systems, depolymerize organic N and hence maintain soil sustainability.

Abbreviations: CM: conventional management; OM: organic management; DGGE: denaturing gradient gel electrophoresis; qPCR: real-time quantitative PCR detecting system; COFCC: China organic food certification center; CCA: canonical correspondence analysis  相似文献   

3.
Microorganisms, capable of proteolysis, are widely distributed in soil but almost nothing is known about the abundance of genes related to protein degradation and the regulation of their activity in terrestrial ecosystems. Therefore, the aim of this study was: (1) to quantify two bacterial genes involved in protein degradation, (2) to investigate factors affecting the abundance of these genes, and (3) to relate this data to potential proteolytic activities. For this purpose, an arable field in southern Germany under integrated management was studied. The uniformly managed field showed pronounced soil heterogeneity with four different soil types. In April, July and October 2003, soil samples were taken from the four soil types at three different depths. We applied a real-time PCR assay for quantification of subtilisin (sub) and neutral metalloprotease (npr) genes, both encoding for extracellular proteases, as well as the 16S rRNA gene representing a rough estimate of the size of the bacterial populations. Potential proteolytic activity was measured using casein as a substrate. Both soil type and time of sampling influenced the size and activity of the bacterial protease genes under investigation. Total nitrogen and carbon availability was, beside soil texture, the main factor responsible for the observed changes in the abundance of proteolytic genes and potential proteolytic activity. Whereas a positive relationship was found between sub and npr gene copy numbers and the number of 16S rRNA gene copies in all cases, a positive relationship between sub and npr coding genes and potential proteolytic activity was only found for sandy soils. This indicates that sandy soils cannot stabilize proteolytic enzymes and the activity of npr and sub genes is strictly dependent on the presence of the corresponding genes. In contrast, in clay soils proteolytic activity was not correlated with the abundance of the genes analyzed, probably due to the stabilization of the proteolytic enzymes.  相似文献   

4.
《Applied soil ecology》2005,28(3):191-201
Annual plant species differ in their rhizosphere microbial community composition. However, rhizosphere communities are often investigated under controlled conditions, and it is unclear if perennial plants growing in the field also have rhizosphere communities that are specific to a particular plant species. The aim of our study was to determine the bacterial community composition of three species of Banksia (B. attenuata R. Brown, B. ilicifolia R. Brown and B. menziesii R. Brown) growing in close proximity in a native woodland in Western Australia and to relate community structure to function. All three species are small trees that produce cluster roots in the field following winter rains. Cluster roots and rhizosphere soil were sampled in early spring (August 2001) and again four weeks later (September 2001). Many new cluster roots were formed in the period between the August and the September sampling. Rhizosphere soil pH, percent soil moisture and C and N content did not differ significantly among species or sampling times. However, the bacterial community composition on the cluster roots and in the rhizosphere soil, studied by denaturing gradient gel electrophoresis (DGGE), differed among the three species, with cluster root age class (young or mature to senescing) and also between sampling times. These changes in community composition were accompanied by changes in the activity of some of the enzymes studied. The activities of β-glucosidase and protease increased over time. The three species differed in asparaginase activity, but not in the activity of acid and alkaline phosphatase in the rhizosphere. These results suggest a relationship between the changes in composition and function of bacterial communities.  相似文献   

5.
The contents and the compositions of the pyrrolizidine alkaloid (PA) complex of ragwort (Senecio jacobaea L.) were examined as potential drivers of fungal community structure in the rhizosphere. S. jacobaea plants within the coastal sand dune reserve of Meijendel (the Netherlands) were assayed for concentration and composition of PAs in roots. Rhizosphere soil was collected from pre-flowering plants, which differed up to 8-fold in PA production, and represented both jacobine and senecionine/seneciphylline chemotypes. Bulk soil samples from the same site were also collected for comparative examination. A culture-independent approach, involving direct DNA isolation, PCR of fungal 18S rRNA genes, and denaturing gradient gel electrophoresis (DGGE), was applied to compare the fungal communities of plants with different PA contents, as well as differences between bulk and rhizosphere samples. Cluster analysis of PCR-DGGE profiles revealed no clear evidence for PA-induced selection of specific fungal communities. However, canonical variance analysis showed that fungal communities associated with high-PA jacobine chemotypes could be discriminated from low PA samples and from the senecionine/seneciphylline chemotypes. The diversity of DGGE banding patterns, both in terms of band number and evenness, showed a trend toward lower diversity in the rhizosphere of high-PA plants as compared to low-PA plants and bulk soil. These results indicate that PA chemotypes of S. jacobaea differ in their influence on soil-borne fungal communities, with jacobine-containing plants exerting a greater selection in the rhizosphere than plants containing senecionine/seneciphylline.  相似文献   

6.
The relative importance of specific plant properties versus soil characteristics in shaping the bacterial community structure of the rhizosphere is a topic of considerable debate. Here, we report the results of a study on the bacterial composition of the rhizosphere of the wild plant Carex arenaria (sand sedge) growing at 10 natural sites in The Netherlands. The soil properties of the sandy soils at these sites were highly disparate, most notably in pH, chloride and organic matter content. Rhizosphere and bulk soil bacterial communities were examined by culture-independent means, namely, 16S rDNA-directed PCR-DGGE profiling. Large differences were observed between the bacterial communities of the different sites for both bulk and rhizosphere soil. Cluster analysis of bacterial profiles revealed that the rhizosphere community of each site was generally more closely related to the bulk soil community of that site rather than to rhizosphere communities of other sites. Hence, bacterial community structure within the rhizosphere of C. arenaria appeared to be determined to a large extent by the bulk soil community composition. This conclusion was supported by a reciprocal planting experiment, where C. arenaria shoots of different sites yielded highly similar rhizosphere communities when planted in the same soil.  相似文献   

7.
《Applied soil ecology》2007,37(2-3):147-155
A number of studies have reported species specific selection of microbial communities in the rhizosphere by plants. It is hypothesised that plants influence microbial community structure in the rhizosphere through rhizodeposition. We examined to what extent the structure of bacterial and fungal communities in the rhizosphere of grasses is determined by the plant species and different soil types. Three grass species were planted in soil from one site, to identify plant-specific influences on rhizosphere microbial communities. To quantify the soil-specific effects on rhizosphere microbial community structure, we planted one grass species (Lolium perenne L.) into soils from three contrasting sites. Rhizosphere, non-rhizosphere (bulk) and control (non-planted) soil samples were collected at regular intervals, to examine the temporal changes in soil microbial communities. Rhizosphere soil samples were collected from both root bases and root tips, to investigate root associated spatial influences. Both fungal and bacterial communities were analysed by terminal restriction fragment length polymorphism (TRFLP). Both bacterial and fungal communities were influenced by the plant growth but there was no evidence for plant species selection of the soil microbial communities in the rhizosphere of the different grass species. For both fungal and bacterial communities, the major determinant of community structure in rhizospheres was soil type. This observation was confirmed by cloning and sequencing analysis of bacterial communities. In control soils, bacterial composition was dominated by Firmicutes and Actinobacteria but in the rhizosphere samples, the majority of bacteria belonged to Proteobacteria and Acidobacteria. Bacterial community compositions of rhizosphere soils from different plants were similar, indicating only a weak influence of plant species on rhizosphere microbial community structure.  相似文献   

8.
Despite the importance of the rhizosphere for nutrient turnover, little is known about the spatial patterns of organic phosphorus mineralization by plants and by microorganisms in the rhizosphere. Therefore, the distribution of acid and alkaline phosphatase activity and the abundance of bacteria belonging to various bacterial phyla were investigated in the rhizosphere of barley (Hordeum vulgare L.) as dependent on the availability of inorganic P. For this purpose, we conducted a greenhouse experiment with barley growing in inclined boxes that can be opened to the bottom side (rhizoboxes), and applied soil zymography and fluorescence-in situ-hybridization (FISH). Acid phosphatase activity was strongly associated with the root and was highest at the root tips. Due to P fertilization, acid phosphatase activity decreased in the bulk soil, and less strongly in the rhizosphere. Alkaline phosphatase activity, i.e., microbial phosphatase activity was high throughout the soil in the control treatment and was reduced due to inorganic P fertilization especially in the rhizosphere and less strongly in the bulk soil. P-fertilization slightly increased the total number of bacteria in the rhizosphere. Moreover, P-fertilization decreased the abundance of Firmicutes and increased the abundances of Beta- and Gamma-Proteobacteria. The total number of bacterial cells was significantly higher at the root surface than at the root tip and at a distance of 30 μm from the root surface. Our results show that alkaline phosphatase activity decreased more strongly in the rhizosphere than in the bulk soil due to P fertilization, which might be because of greater C deficiency in the bulk soil compared to the rhizosphere. Furthermore, the results indicate a spatial separation between hotspots of acid phosphatase activity and hotspots of bacteria in the rhizosphere of H. vulgare. Taken together, our study shows that bacteria and phosphatase activity were very heterogeneously distributed in soil, and that the effects of P fertilization on phosphatase activity differed strongly between bulk soil and rhizosphere as well as between various zones of the rhizosphere.  相似文献   

9.
We evaluated changes occurring in the rhizosphere microbial communities of Scots pine (Pinus sylvestris L.) due to tree-felling and decrease of the photosynthetic C flow into the soil under field conditions over one growing season. Samples were taken from tree rhizospheres, freshly felled stump rhizospheres and bulk soil. We used culture dependent (CFU counts, community level physiological profiles, CLPPs) and independent methods (fluorogenic MUF-substrates, PLFA pattern and PCR-DGGE) to monitor the microbial communities in soil samples. The numbers of cultivable bacteria and amounts of phosphatase activity in the rhizosphere of trees were significantly higher compared with those in the bulk soil. The organic C consuming community measured by CLPP was stimulated directly after the tree-felling in stump rhizospheres; utilization of the disintegration components of cellulose, hemicellulose and chitin increased. Furthermore, bacterial and fungal biomass as well as chitin decomposers (CFU) increased in the stump rhizosphere. After 11 weeks of tree-felling the stump rhizosphere soluble PO4-P and NH4-N as well as amounts of total C and N began to resemble the concentrations measured in the bulk soil. However, the stump rhizosphere community structure detected by PLFA and PCR-DGGE still resembled that of the tree rhizosphere.  相似文献   

10.
The SOM field experiments in Kenya, which have been initiated in 2002 on two contrasting soils (clayey Humic Nitisol (sand: 17%; silt: 18%; clay: 65%) at Embu, sandy Ferric Alisol (sand: 66%; silt: 11%; clay: 22%) at Machanga), were used for exploring the effect of nine year annual application of biochemically contrasting organic inputs (i.e., Zea mays (ZM; C/N ratio: 59; (lignin + polyphenols)-to-N ratio: 9.8); Tithonia diversifolia (TD; 13; 3.5); Calliandra calothyrsus (CC; 13; 6.7)) on the soil bacterial decomposer community. Soil samples were taken at the onset of the rainy season before application of fresh organic inputs in March 2011. We studied the abundance (quantitative PCR) and community structure (T-RFLP analysis) of the total (i.e., 16S rRNA gene) and specifically proteolytic (i.e., npr gene encoding neutral metalloproteases) bacteria. Alterations of the soil microbial decomposer community were related to differences of quantity (i.e., soil carbon (TC)) and particularly composition of SOC, where mid-infrared spectroscopic (DRIFTS) information, and contents of extractable soil polyphenol (PP) and the newly introduced PP-to-TC ratio served as SOC quality indicators. For total bacteria, effect of organic input quality was minor in comparison to the predominant influence of soil texture. Elevated soil PP content, driven by polypheneol rich organic inputs, was not suppressive for overall bacterial proliferation, unless additional decomposable C substrates were available as indicated by PP-to-TC ratios. In contrast to the total bacterial community, biochemical quality of organic inputs exposed a stronger effect on functionally specialized bacterial decomposers, i.e., proteolytic bacteria. The npr gene abundance was depressed in the TD treated soils as opposed to soils receiving CC, and showed a positive correlation with soil PP. It was suggested that the high presence of lignin and polyphenol relative to the N content in organic inputs was increasing the npr gene abundance to counteract most likely the existence of polyphenol–protein complexes aggravating protein degradation. We concluded from our study that integration of spectroscopic, geochemical (i.e., soil PP) and molecular soil data provides a novel pathway to enhance our understanding of the lasting effect of organic input quality induced SOC quality changes on bacterial decomposers and particularly proteolytic bacteria driving soil organic N cycling.  相似文献   

11.
Rice roots provide a specific habitat for microorganisms in the rhizosphere of a submerged field through supply of oxygen and organic matter. Many studies have focused on the microbial community in the rice rhizosphere, but less is still known about the microeukaryotic community structure of rice rhizosphere. This study explored the microeukaryotic community structure of a rice rhizosphere through denaturing gradient gel electrophoresis (DGGE) targeting 18S rRNA gene. The rice roots and the rhizosphere soil samples, which were collected from a field under rice-wheat rotation system, were separately analyzed. To characterize the rice rhizosphere-specific community, the bulk soil of rice field and the wheat rhizosphere samples were also examined. DGGE fingerprints showed that the microeukaryotic community of rice roots were distinct from the community of the bulk soil and showed a temporal shift with the growth stage. The rhizosphere soil community was distinct from the root and bulk soil communities, but this could be explained by that the root and bulk soil communities were shared in the rhizosphere. The rice rhizosphere community was also distinct from those in the wheat rhizosphere. Microeukaryotes that characterized the rice rhizosphere (roots and the rhizosphere soil) community could be affiliated to Polymyxa, flagellates, and oomycetes, which suggested that microeukaryotes with various ecological roles, e.g., parasites, bacterial grazers, and decomposers, inhabit the rice rhizosphere. The results showed that the rice root and its growth stages are key factors shaping the microeukaryotic community structure in the rhizosphere.  相似文献   

12.
Pea plants were grown in γ-irradiated soil in pots with and without addition of the AM fungus Glomus intraradices at sufficient N and limiting P. Depending on the growth phase of the plant presence of AM had negative or positive effect on rhizosphere activity. Before flowering during nutrient acquisition AM decreased rhizosphere respiration and number of protozoa but did not affect bacterial number suggesting top-down regulation of bacterial number by protozoan grazing. In contrast, during flowering and pod formation AM stimulated rhizosphere respiration and the negative effect on protozoa decreased. AM also affected the composition of the rhizosphere bacterial community as revealed from DNA analysis (DGGE). With or without mycorrhiza, rhizosphere respiration was P-limited on very young roots, not nutrient limited at more mature roots and C-limited at withering. This suggests changes in the rhizosphere community during plant growth also supported by changes in the bacteria (DGGE).  相似文献   

13.
Differences in the culturable fractions of total and metal-tolerant bacteria inhabiting bulk soil of a metal-mine spoil heap and the rhizosphere of silver birch (Betula pendula) or bushgrass (Calamagrostis epigejos), completed with changes in total microbial community structure in the soil, were assessed by MIDI-FAME (fatty acid methyl ester) profiling of whole-cell fatty acids. In addition, the abundance of metal-tolerant populations among the culturable bacterial communities and their identity and the metal-tolerance patterns were determined. The high proportions of Cu- and Zn-tolerant bacteria that ranged from 60.6% to 94.8% were ascertained in the heap sites. Within 31 bacterial isolates obtained, 24 strains were Gram-positive and Arthrobacter, Bacillus, Rathayibacter, Brochothrix, and Staphylococcus represented those identified. Minimum inhibitory concentration (MIC) data indicated that several strains developed multi-metal tolerance, and the highest tolerance to Cu (10 mM) and Zn (12 mM) was found for Pseudomonas putida TP3 and three isolated strains (BS3, TP12, and SL16), respectively. The analysis of FAME profiles obtained from the culturable bacterial communities showed that Gram-positive bacteria predominated in bulk soil of all heap sites. In contrast, the rhizosphere communities showed a lower proportion of the Gram-positive group, especially for silver birch. For the total microbial community, mostly Gram-negative bacteria (e.g., Pseudomonas) inhabited the heap sites. The results suggest that the quantitative and qualitative development of heterotrophic microbiota in the soil of the metal-mine spoil heap seems to be site-dependent (i.e., rhizosphere vs. bulk soil), according to differences in the site characteristics (e.g., enrichment of nutrients and total metal concentrations) and impact of plant species.  相似文献   

14.
Abstract

Protease-active extracts were prepared from 2 rice-rhizosphere and non-rhizosphere soil samples 3 proteolytic isolates from the soil samples which were taken from water logged paddy fields (Gray Lowland Soils) under double cropping of rice and wheat that had been treated with chemical fertilizer (CF) or organic manure (OM). The activities were assayed and characterized using an artificial substrate, benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-leucine (ZFTL). The protease-active extracts from the rhizosphere and non-rhizosphere of the CF and OM soils (abbreviated as CFR, CFN, OMR, and OMN) showed an optimum pH between 7 and 8. The extracts hydrolyzed ZFTL to Tyr-Leu and Leu; generally, Tyr-Leu was the main reaction product. Activities of all the samples were inhibited by EDTA and p-chloromercuribenzoic acid (PCMB) but not by pepstatin and phenylmethanesulfonyl fluoride (PMSF). These results indicate that the main components of the ZFTL-hydrolases in CFR, CFN, 0MR, and OMN were essentially similar.

Optimum pH for the activity of the protease extracted from a Gram negative rod (MNB003)., two actinomycetous isolates (MNA014., MRA017) was about 8, 8, and 8.5, respectively. Extract from the culture of MNB003 hydrolyzed ZFTL to Tyr-Leu., Leu,., the protease activity was inhibited by EDTA., PCMB. Protease activities of the extracts from cultures of the two actinomycetes were inhibited by EDTA but not by PCMB. The extract from culture of MNA014 produced mainly Tyr-Leu from ZFTL while MRA017 produced Leu. Characteristics of the protease activity from cultures of MNB003., MNA014 were similar to those of the enzyme extracted from a paddy field soil under single cropping (Takeuchi., Hayano 1994: Soil Sci. Plant Nutr., 40, 691-695) and from an Andosol under tomato cultivation (Hayano et al. 1987: Biol. Fertil. Soils, 4, 179-183), respectively.  相似文献   

15.
Although root cap cells are an important substrate for microorganisms in the rhizosphere, little attention has been paid to the decomposition of sloughed root cap cells by microorganisms. This study used rice plant callus cells grown on medium containing 13C-labelled glucose as a model material for rice plant root cap cells. Harvested 13C-labelled callus cells (78 atom % 13C) were subjected to decomposition in an aerobic soil microcosm for 56 days. The low cellulose and lignin levels and the disaggregated nature of the callus cells indicated that these cells were an appropriate model material for root cap cells. DNA was extracted from a soil incubated with 12C- and 13C-callus cells and subjected to buoyant density gradient centrifugation to identify bacterial species that assimilated carbon from the callus cells. The stability of the total bacterial communities during the incubation was estimated. Many DGGE bands in light fractions of soil incubated with 13C-callus cells were weaker in intensity than those from soil incubated with 12C-callus cells, and those bands were shifted to heavier fractions after 13C-callus treatment. 13C-labelled DNA was detected from Day 3 onwards, and the DGGE bands in the heavy fractions were most numerous on Day 21. DGGE bands from heavy and light fractions were sequenced, revealing more than 70% of callus- C incorporating bacteria were Gram-negative, predominantly α-Proteobacteria, β-Proteobacteria, γ-Proteobacteria, Sphingobacteria and Actinobacteria. These species were phylogenetically distinct from the bacteria reported to be present during plant residue decomposition and resident in rice roots. This study indicates that root cap cells are decomposed by specific bacterial species in the rhizosphere, and that these species augment the diversity of rhizospheric bacterial communities.  相似文献   

16.
Fusilade (fluazifop-P-butyl) is an extensively used herbicide in legumes cropping systems; nevertheless, the extent of its ecological risk remains unknown. The aim of this work is to estimate the impact of this herbicide on some key enzyme activities, namely acid phosphatase, urease and FDA, and the effect on soil bacterial communities in the rhizosphere of pea (Pisum sativum). Acid phosphatase, urease and FDA showed a high decrease following the application of fluazifop-P-butyl mainly at ten-fold field rate. Grain yield was also severely affected at this concentration (−50%). T-RFLP analysis of 16S rDNA revealed a significant effect of fluazifop-P-butyl application on richness and structure of soil bacterial communities. Putative taxonomic assignation of induced TRFs revealed a marked pattern of acidogenic bacteria like as Staphylococcus, Escherichia, Desulfobacter, Clostridium, Paludibacter and other sulfate-reducing bacteria like as Desulfocella halophila and Desulfocurvus vexinensis. These bacteria may participate in the acidogenesis and the production of propionic acid from the fluazifop-P-butyl after hydrolytic cleavage. They are also known to play important roles in the global biogeochemical sulfur cycles. Unfortunately, some potential human pathogens were also stimulated. Moreover, many putative plant-growth-promoting bacteria belonging to the genera Streptomyces and Bacillus were found to be inhibited in the bulk soil and rhizosphere. The fluazifop-P-butyl effect on bacterial diversity was more pronounced in the rhizosphere compared to bulk soil or uncultivated soil which may suggest that a major component of this effect is mediated by the root system.  相似文献   

17.
A better understanding of the relationships among different cropping systems, their effects on soil microbial ecology, and their effects on crop health and productivity is necessary for the development of more efficient, sustainable crop production systems. We used denaturing gradient gel electrophoresis (DGGE) to determine the impacts of crop rotations and crop types on bacterial and fungal communities in the soil. The communities of bacterial 16S rRNA genes and fungal 18S rRNA genes were analyzed in experimental field plots that were kept under 4 different crop rotation systems from 1999 to 2008 (continuous cabbage (Brassica oleracea var. capitata L.), cabbage–lettuce (Lactuca sativa L.) rotation, cabbage–radish (Raphanus sativus L. var. longipinnatus L.H. Bailey) rotation, and a 3-year crop rotation). A principal component analysis (PCA) and a canonical correspondence analysis (CCA) revealed that both the bacterial and fungal communities in bulk soils were influenced by the crop rotation systems. However, the primary factors influencing each community differed: bacterial communities were most affected by soil properties (especially carbon content), while fungal communities were influenced most strongly by rotation times. To elucidate factors that may cause differences in crop rhizosphere microbial communities, the microbial communities in the harvested cabbage rhizospheres were also analyzed. The results suggest that the fungal communities in bulk soil are related to the rhizosphere fungal communities. Our present study indicates that the microbial communities in bulk and rhizosphere soils could be managed by crop rotation systems.  相似文献   

18.
Silver nanoparticles hold great promise as effective anti-microbial compounds in a myriad of applications but may also pose a threat to non-target bacteria and fungi in the environment. Because microorganisms are involved in extensive interactions with many other organisms, these partner species are also prone to indirect negative effects from silver nanoparticles.Here, we focus on the effects of nanosilver exposure in the rhizosphere. Specifically, we evaluate the effect of 100 mg kg−1 silver nanoparticles on maize plants, as well as on the bacteria and fungi in the plant's rhizosphere and the surrounding bulk soil. Maize biomass measurements, microbial community fingerprints, an indicator of microbial enzymatic activity, and carbon use diversity profiles are used. Hereby, it is shown that 100 mg kg−1 silver nanoparticles in soil increases maize biomass, and that this effect coincides with significant alterations of the bacterial communities in the rhizosphere. The bacterial community in nanosilver exposed rhizosphere shows less enzymatic activity and significantly altered carbon use and community composition profiles. Fungal communities are less affected by silver nanoparticles, as their composition is only slightly modified by nanosilver exposure. In addition, the microbial changes noted in the rhizosphere were significantly different from those noted in the bulk soil, indicated by different nanosilver-induced alterations of carbon use and community composition profiles in bulk and rhizosphere soil.Overall, microorganisms in the rhizosphere seem to play an important role when evaluating the fate and effects of silver nanoparticle exposure in soil, and not only is the nanosilver response different for bacteria and fungi, but also for bulk and rhizosphere soil. Consequently, assessment of microbial populations should be considered an essential parameter when investigating the impacts of nanoparticle exposure.  相似文献   

19.
Sulfonates are major soil sulfur components that can be desulfurized by certain soil-borne bacteria as a sulfur source, but application of sulfate through fertilization could affect this natural sulfur mobilizing process. This study investigates the effects of sulfate treatments on bacterial communities in semi-natural grasslands. Agrostis stolonifera-dominated turfs with their autochthonous bacteria from Woburn Experimental Farm, UK, were incubated with and without additional sulfate over a period of eight weeks and compared to soil and rhizosphere samples taken from the field directly. Cultivable rhizobacteria able to desulfurize toluenesulfonate were dominated by strains affiliated to the Variovorax, Polaromonas and Rhodococcus genera. The betaproteobacteria communities (16S rRNA gene-based denaturing gradient gel electrophoresis) and the desulfonating bacterial communities (asfA based terminal restriction fragment length polymorphism) revealed clear differences between field rhizospheres and bulk soil and the two types of incubated rhizospheres. Clone libraries of asfA from the sulfate-limited treatment were dominated by a new AsfA type, affiliated to Polaromonas. The results from this study suggest that the community of desulfonating bacteria in the Agrostis rhizosphere adapts quickly to changing levels of inorganic sulfate.  相似文献   

20.
Three experiments were conducted in this study in order to investigate the impacts of soil type, soybean genotype, and the reproductive growth stage on bacterial communities in the soybean rhizosphere. Communities were evaluated by principal component analysis of denaturing gradient gel electrophoresis (DGGE) banding patterns and sequencing of partial 16S rDNA polymerase chain reaction (PCR) amplicons. A pot experiment analyzing three soybean genotypes grown in two different types of soil (Black soil and Dark Brown soil) indicated that soil type was the major factor in influencing the bacterial communities in the soybean rhizosphere, with a more significant effect observed in the Black soil samples than in the Dark Brown soil samples. A field experiment was conducted in Dark Brown soil using three soybean genotypes, and the results gleaned from both pot and field experiments indicated that bacterial communities in the soybean rhizosphere changed with growth stages, and higher number of DGGE bands observed in early reproductive growth stages, while surprisingly, a significant impact of genotype on the bacterial communities was not observed in these experiments. However, a plate culture experiment targeting the culturable bacterial communities detected a remarkable difference in the community structures of the rhizosphere between the two soybean genotypes, suggesting that a small portion of the total bacteria was influenced by genotype. Sequence analysis of DGGE bands indicated that bacterial phyla of Proteobacteria, Actinobacteria, Bacteroidetes, Nitrospirae, Firmicutes, Verrucomicrobia and Acidobacteria commonly inhabit the soybean rhizosphere.  相似文献   

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