共查询到20条相似文献，搜索用时 218 毫秒
1.
2.
3.
硒对奶牛红细胞谷胱甘肽过氧化物酶活力的浓度依赖性研究 总被引：6，自引：0，他引：6
实验通过在缺硒地区奶牛血清中添加一定量外加硒（Na2SeO3）测其谷胱甘肽过氧化物酶活力。结果表明，一定浓度内，硒含量与谷胱甘肽过氧化物酶活力呈正相关，一定量外加硒可以提高血清中GSH－Px活力。从0.01μg/L开始，GSH－Px活力随硒浓度的增加而增加，当外加硒浓度达到0.1μg/L时，GSH－Px活力达到最大值，眩后进一步增加硒浓度，GSH－Px活力非但不增加，呈明显下降趋势。 相似文献
4.
5.
为探讨亚硒酸钠对黄粉虫幼虫血淋巴谷胱甘肽过氧化物酶（GSH—PX）活性的影响,将黄粉虫的中龄幼虫随机分成6组,饲喂含不同浓度的亚硒酸钠饲料。采用DTNB比色法测定GSH—PX活性。结果显示,饲喂含亚硒酸钠的饲料后黄粉虫幼虫血淋巴GSH—PX活力都明显上升：饲喂同一浓度的亚硒酸钠,幼虫血淋巴GSH—PX活力均比对照组高,只有加硒浓度为20mg／kg．80mg／kg在第16d时,黄粉虫血淋巴GSH—PX活力随着饲喂时间的延长而显著上升;饲喂不同浓度的亚硒酸钠,在硒浓度为5mg／kg、10mg／kg时,黄粉虫血淋巴GSH-PX活力随亚硒酸钠浓度的增加显著上升,在硒浓度为20mg／kg时开始下降。 相似文献
6.
基于谷胱甘酞过氧化物酶—GSH—PX(EC. 1.11. 1.9)的活力与硒水平之间相互关系的系统研究,血和组织中GSH—PX活力水平的测定,可作为探测缺硒的一种有用方法。迄今,家畜体内GSH—PX的测定方法大多难以避免艰苦的分离与洗涤过程。采用此法直接进行肝素化全血中GSH—Px的活力测定而效果最佳。此外,在不同温度条件下测定了牛和猪全血中GSH—Px的稳定性。 相似文献
7.
吕渭綸 《广东畜牧兽医科技》1987,(3)
硒(Selenium,Se)为动物体内所必需的微量元素,是动物体内谷胱甘肽过氧化物酶(GSH—PX)的组成成分,动物缺硒能引起这种强氧化剂的酶活性降低,体内代谢过程中产生的有害过氧化物就不能分解而导致生物膜通透性的改变,使微生物容易侵犯而致动物发病,所以硒能增强动物对疾病的抵抗力,这是硒能防治多种畜禽疾病的主要原因。 相似文献
8.
为了研究补硒对硒从母猪转运到仔猪的影响,试验对哺乳母猪饲粮中不补充硒或分别以亚硒酸钠、酵母硒形式补充0．3mg／kg硒,高于NRc对饲粮硒的推荐量（0．15mg／kg）。未强化硒的玉米一豆粕型基础饲粮为阴性对照组,饲粮中含有较高的内源性硒（Se：0．20～0．23mg／kg）。52头母猪从分娩前60d饲喂到哺乳第14d。分别在分娩前60d、30d、分娩当天、产后14d,从每个处理组选择6头母猪采血,测定血清硒浓度。收集分娩后12h内的初乳,及哺乳第14d的乳汁。在仔猪出生和断奶（14d）当天,从每窝仔猪中选择5头（每个处理组12窝仔猪）采血,混合血清,检测硒、免疫球蛋白G的浓度,及谷胱甘肽过氧化物酶的活性。各处理组母猪在妊娠期间的血清硒水平下降;哺乳期间逐渐增加。分娩时,饲喂酵母硒（P〈0．06）的母猪血清硒浓度高于没有补硒的母猪。与饲喂亚硒酸钠的母猪相比,饲喂酵母硒的母猪其初乳和常乳（第14d）中硒浓度增加（P〈0．01）。与饲喂基础饲粮组相比,酵母硒组仔猪在出生时血清硒浓度增加（P〈0．01）。14日龄仔猪血清硒浓度在各个处理组间没有差异。仔猪血清IgG和谷胱甘肽过氧化物酶活性不受饲粮硒源的影响。给妊娠和哺乳母猪补充0．3mg／kg有机硒或无机硒,窝产死胎数减少。但是,只有饲喂有机硒（酵母硒）的母猪,仔猪出生时血清硒水平更高。有机硒组母猪初乳和14d常乳中硒浓度增加程度高于无机硒组。 相似文献
9.
不同硒水平对生长育肥猪生产性能的影响 总被引：3，自引：0，他引：3
试验研究了宁夏常用饲粮中不同硒水平(添加硒0，0．15，0．30，0．45，0．60mg／kg)对生长育肥猪生产性能的影响。结果表明：(1)在饲粮中添加不同水平的硒，对各个饲养阶段生长育肥猪的日增重、日采食量和饲料报酬没有明显影响。但从全期来看。饲粮中添加硒0．30mg／kg时日增重最高。料重比最低。(2)综合肉质品质各项指标的测定结果。饲粮中添加硒0．30，0．45，0．60mg／kg时，肌肉保水性、嫩度较好。以饲粮中添加硒0．30mg／kg时为最好。补硒组肉色评分、肌肉新鲜度和抗应激能力均较缺硒组高，肉色评分以饲粮中添加硒0．30mg／kg时最为理想。(3)在宁夏缺硒地区的玉米-豆粕、胡麻饼型饲粮中。从满足正常生长发育的代谢需要、改善肉的品质及降低发病率等方面综合考虑。在15～90kg体重的生长育肥猪基础饲粮中建议添加0．30mg／kg硒为宜，饲粮硒水平为0．33mg／kg，特别是在15～30kg体重的仔猪阶段。从降低发病率的角度考虑。硒的添加量可适当提高。 相似文献
10.
11.
本文旨在研究一氧化氮(NO)在硒缺乏致鸡脾脏淋巴细胞凋亡中的作用.选择1日龄健康海蓝褐雏鸡80只,随机分为对照组和缺硒组2个组,每组4个重复,每个重复10只鸡,对照组鸡饲料中硒含量为0.282 mg/kg,缺硒组鸡饲料中硒含量为0.032 mg/kg.分别于30、45、60和75日龄每组随机抽取10只鸡采样,测定脾脏和血清谷胱甘肽过氧化物酶(GSH-Px)活性、NO含量、一氧化氮合酶(NOS)活性以及脾脏和全血中硒含量的变化;检测脾脏中诱导型一氧化氮合酶(iNOS)mRNA表达量变化和淋巴细胞的凋亡情况.结果表明,与对照组比较,缺硒组鸡脾脏和全血中硒含量降低;脾脏和血清中GSH-Px活性降低;NO含量、NOS活性及iNOS mRNA表达量升高;脾脏淋巴细胞DNA出现降解,呈现梯形条带,电镜下细胞核皱缩,核染色质边聚、固缩,线粒体肿胀,嵴溶解甚至消失,内质网排列紊乱、肿胀等呈现典型的细胞凋亡现象.本试验结果提示硒缺乏可引起鸡脾脏及血清NO代谢发生改变,导致脾脏淋巴细胞发生凋亡,表明NO在硒缺乏致鸡脾脏淋巴细胞凋亡中具有重要作用. 相似文献
12.
Selenium and glutathione peroxidase levels in lambs receiving feed supplemented with sodium selenite or selenomethionine 总被引：1，自引：0，他引：1
Twenty-one 6 months old female lambs were divided into 7 groups and fed a basal diet containing 0.13 mg Se/kg. The basal diet was further supplemented with 0, 0.1, 0.5 or 1.0 mg Se/kg either as sodium selenite or as selenomethionine, and was fed for 10 weeks. Both feed additives produced an increase in the selenium concentration in the tissues analysed. Significant correlations were found between the concentrations of selenomethionine or sodium selenite added to the feed and the subsequent tissue levels. However, the selenium levels seemed to plateau at approximately 0.5 mg Se/kg of supplemented sodium selenite. The total glutathione peroxidase (GSH-Px) activity of the tissues increased when the selenium supplementation increased from 0 to 0.1 mg/kg for both selenium compounds. With further increase in selenium supplementation the GSH-Px activity in the tissues plateaued except in the blood where the activity continued to rise with increasing selenomethionine supplementation. The selenium dependent GSH-Px activity in the liver rose with increasing selenomethionine supplementation, but approached a plateau when 0.1 mg Se/kg as sodium selenite was added to the feed. The selenium concentration in whole blood responded more rapidly to the selenium supplementation than did GSH-Px activity. The experiment indicates that the optimal selenium concentration in the feed is considerably higher than 0.1 mg Se/kg, and that selenium levels of 1.0 mg/kg in the feed do not result in any risk for the animals or the consumers of the products.Key words: dietary selenium, lambs, selenium concentrations, glutathione peroxidase activities, tissues 相似文献
13.
低硒雏鸡口服亚硒酸钠后机体硒浓度及谷胱甘肽过氧化物酶的活性动态变化规律 总被引：2，自引：0，他引：2
低硒雏鸡口服亚硒酸钠后，对不同时间血液（清）、肝、肾、胰、心、脾硒浓度及谷胱甘肽过氧化物酶活性的动态变化规律测定。结果发现随着硒浓度的变化，酶活性也发生相应变化，但１９２ｈ后肾、脾酶活性出现第２次升高而其硒浓度仍降低。值得注意的是酶活性的变化总滞后于硒浓度的变化 相似文献
14.
The response of cats to dietary sodium selenite (Na(2) SeO(3)) and organically bound selenium was studied in two separate studies with four cats per treatment and three levels of selenium supplementation (targets 1.0, 1.5 and 2.0 μg/g DM) for each Se source. Whole blood and plasma selenium concentrations and glutathione peroxidase (GPx) activity were determined at 7-time points across the 32-day study. Faeces were quantitatively collected during the last 8 days and urine was collected daily during both studies. The basal diet used had a low apparent faecal selenium absorption of 25.3 ± 3.0%. Daily faecal and urinary selenium excretion increased linearly with increasing selenium intake for both Se sources. Urinary selenium concentration of the cats fed the supplemented diets increased rapidly (～2 days) and remained constant throughout the remainder of the study. Apparent faecal selenium absorption was high for both selenium sources (73.2% and 80.0%). Plasma, and to a lesser extent, whole blood selenium concentrations increased in a dose-dependent manner with supplementation. Whole blood and plasma GPx activity were highly variable and showed a variable response to dietary selenium intake. Cats closely regulate selenium homeostasis through increasing urinary excretion whilst faecal absorption remains unaffected. 相似文献
15.
Juhani Hakkarainen Paul Lindberg Gsta Bengtsson Lennart Jnsson 《Acta veterinaria Scandinavica》1978,19(2):269-284
Blood serum glutathione peroxidase activity and blood selenium concentration were measured in blood samples from pigs subjected to experimentally induced selenium deficiency and dietary selenium supplementation on graded levels. A highly significant correlation between blood selenium and serum GSH-Px activity in pigs, especially in selenium deficient pigs, was demonstrated. There was also a strong relationship between blood selenium concentration and serum GSH-Px activity in pigs receiving dietary selenium at graded levels. Serum GSH-Px activity exhibited an excellent close-response relationship to dietary selenium. Linear regression analysis showed that the increased serum GSH-Px activity was a function of the dietary selenium concentration. The fitness of serum in monitoring slight changes of the selenium status of pigs with help of the estimation of GSH-Px activity was discussed. The measurement of serum GSH-Px activity seems to provide a useful and rapid means for defining selenium requirements and for identifying selenium deficiency in growing pigs. 相似文献
16.
Blood selenium levels were found to fluctuate throughout the year, being highest during the indoor season when the greatest amounts of compound concentrates were fed. From October to January the average blood selenium levels increased from 0.10 μg/ml to 0.18 μg/ml (15 cows). Subcutaneous injections of barium selenate (500 mg selenium) increased the blood selenium levels significantly. The treated group (15 cows) reached average levels of about 0.21 μg/ml blood during the indoor season.The effect of oral supplementation of sodium selenite (for 2 months) on the levels of plasma selenium, blood selenium and glutathione peroxidase activity was investigated. Plasma selenium was found to give an immediate reflection of the daily selenium intake. Maximum activity of glutathione peroxidase was reached 1 month after the end of the supplementation period.It is concluded that if selenium enriched concentrates are used in a normal feeding regimen, further supplementation with selenium does not seem to be necessary. 相似文献
17.
Effects of oral selenium supplementation on mastitis markers and pathogens in Estonian cows 总被引：1，自引：0，他引：1
Malbe M Klaassen E Kaartinen L Attila M Atroshi F 《Veterinary therapeutics : research in applied veterinary medicine》2003,4(2):145-154
The effects of selenium supplementation on mastitis parameters in milk and on glutathione peroxidase (GPx) levels in blood were evaluated. Fifty-five Estonian dairy cows were allocated to selenium-supplemented (n=39) and nonsupplemented (n=16) groups. The supplemented group received 0.2 ppm organic selenium in the form of selenium yeast in their diet daily for 8 weeks. The nonsupplemented cows received their standard diet with no selenium supplementation. Mastitis parameters (i.e., bacteriologic findings and somatic cell count, N-acetyl-beta-D-glucosaminidase, and bovine serum albumin concentration) and GPx levels were monitored. The increase in the activity of GPx was significantly (P<.001) greater in selenium-supplemented cows than in nonsupplemented ones. Milk samples from each quarter were examined before and 8 weeks after initiation of the study. The proportion of quarters still pathogen-free after 8 weeks was significantly (P<.01; odds ratio [OR]=9.7) higher in selenium-supplemented cows than in nonsupplemented cows. However, when quarters milk-tested as pathogen-infected at the start of the study were reexamined after 8 weeks, there was no significant (P=.14; OR 3.6) difference in the proportion of pathogen-free quarters between selenium-supplemented cows and nonsupplemented cows. Differential positive rate (Youden's index) revealed that individual quarters were more prone to be infected by pathogens when the blood GPx activities in cows were below the cutoff value of 3.3 microkat/g hemoglobin than when GPx activity was above this value. It was concluded that selenium supplementation in cows with low GPx activity seems to support udder defense mechanisms that favor reduction of the incidence of new mastitis cases. 相似文献
18.
Studies on serum tocopherol, selenium levels and blood glutathione peroxidase activities in calves with white muscle disease 总被引：1，自引：0，他引：1
Y Hoshino S Ichijo S Osame E Takahashi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(4):741-748
For the purpose of clarifying the cause of white muscle disease (WMD) in calves, tocopherol and selenium levels and blood glutathione peroxidase (GSH-Px) activity were measured on 10 calves with WMD and nine of their dams. The main clinical symptoms of the 10 calves with WMD were motor disturbances including recumbency and stiffness. Serum enzyme activities (GOT, GPT, CPK, LDH) in calves with WMD increased markedly, and this increase was also observed in some of their dams. Serum tocopherol levels of calves with WMD were low, 70% of which showing deficient levels of less than 70 micrograms/100 ml. Serum selenium levels of all the calves were lower than 35 ppb, indicating a deficiency, and were accompanied by low blood GSH-Px activity. alpha-Tocopherol and selenium concentrations in organs were very low. Dams of calves with WMD showed low serum tocopherol levels, 22% of which indicating deficient levels below 150 micrograms/100 ml. Serum selenium levels in dams showed a marked decrease to under 20 ppb, and also low blood GSH-Px activity. Feedstuffs supplied in the farms to affected calves indicated very low alpha-tocopherol contents (below 3 mg/100g DM) and low selenium concentrations below 50 ppb in DM. It was concluded that WMD in calves was attributable to nutritional muscular dystrophy caused by deficiencies in tocopherol and selenium in feedstuffs supplied to their dams. 相似文献
19.
Pregnant sows were injected subcutaneously (s.c.) or intramuscularly (i.m.) with a barium selenate suspension (0.5–1.0 mg Se/kg body weight (b.w.)) and together with control animals fed a commercial diet. No response to the injection was seen either in blood selenium levels or in glutathione peroxidase (GSH-Px) activity in the sows. There was, however, a significant difference in these parameters between piglets born from treated dams and control animals. This status was maintained during the nursing period. In another experiment pigs (20 kg b.w.) on a Se-deficient diet were injected s.c. and i.m. with barium selenate (2.5 mg Se/kg b.w.). The treated groups maintained their blood levels of selenium and GSH-Px activity, although the selenium values in the group treated intramuscularly started to decline after 4 weeks. Organ samples from both groups were equal with regard to selenium at the time of slaughter while the control group showed a rapid decline both in blood selenium levels and GHS-Px activity. 相似文献
20.
The effect of varying levels of dietary vitamin E on selenium status was determined using 40 Wistar rats with similar initial body weight. The rats were equally divided into four groups and fed the following dietary treatments (mg vitamin E/kg DM): 18 (control; C‐diet), 0 (0E‐diet), 9 (0.5E‐diet) and 36 (2E‐diet) for either 4‐week (Phase 1) or 8‐week (Phase 2) period. At the end of experiment, animals were slaughtered to measure vitamin E and selenium levels in the brain, liver, spleen, kidney, muscles and blood tissues. Dietary vitamin E levels did not affect feed and water intake and body weight. But whole‐blood selenium concentration in rats fed 0E‐diet was higher than in rats fed 2E‐diet after 4 weeks. Selenium level in muscle, spleen and brain was also higher (p < 0.05) in rats fed the 0E‐diet than in rats fed C‐diet after 4 weeks of feeding. On the other hand, selenium level in the muscle was lower (p < 0.05) in rats fed 0.5E‐diet than in those fed 0E‐diet after 4 weeks of feeding, but not after 8 weeks of feeding. Increasing dietary vitamin E level directly influenced selenium content of the spleen and brain after 8 weeks of feeding 2E‐diet. Moreover, the twofold increase in vitamin E intake resulted in a tendency to reduce whole‐blood selenium level and total selenium in the liver and kidney after 4 and 8 weeks. The results showed that the increasing dietary vitamin E level resulted in a tendency to reduce Se contents in some vital organs of the body such as the liver and kidney, suggesting their close compensatory interrelationship. Therefore, dietary vitamin E level directly influenced selenium metabolism in the animal body. 相似文献