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1.
Mark P. Broderick Lucy D. Leake Martyn G. Ford Richard Greenwood Raymond Crichton 《Pest management science》1991,32(1):57-72
A method is described for simultaneous recording of in-vivo nerve activity and toxicological symptoms from 6th-instar larvae of Spodoptera littoralis poisoned with insecticide. Topically applied doses (9 ng-180 μg insect?1) of cypermethrin induced hyperactivity, repetitive activity and bursting in the ventral nerve cord; these activities were associated with different stages of poisoning in the whole animal and are consistent with pharmacokinetic studies. Nerve activity continued long after the larvae appeared to be dead (i.e. up to 5-6 days), although microscopic examination of these larvae revealed that they were still capable of small movements. At the lower doses (up to the LD95) time to death was inversely proportional to dose. Death of the organism may be related to the number of neurones which are affected by the insecticide. 相似文献
2.
The development of resistance to an insecticide under various types of application method has yet to be reported in the literature. Five fall armyworm Spodoptera armigera (JE Smith) colonies were reared in a chamber for ten generations before starting topical application bioassays. From each colony, 200-500 third-fourth-instar larvae were fed for 72 h on corn plants sprayed with cypermethrin or spinosad at minimum application rate (20 g ha(-1)) using a small droplet size nozzle XR8001VS (volume median diameter D(v0.5) = 163 microm) or a large droplet size nozzle XR8008VS (D(v0.5) = 519 microm). Surviving larvae were transferred to untreated corn leaves to complete their life cycle. Next-generation third-instar larvae of each colony were topically dosed with technical cypermethrin or spinosad at 1 microL per larva, and mortality was recorded 24 h post-treatment. The results indicated that cypermethrin demonstrated an insecticidal activity greater than that of spinosad, and the cypermethrin regression lines moved to the right faster than those for spinosad, indicating an increased tolerance of cypermethrin. Generally, larvae from all generations (F1-F7) under the XR8008VS treatments were less susceptible to cypermethrin and developed resistance faster and to higher levels than larvae from the XR8001VS treatments. The confidence limits (95%) of LD(50) for all spinosad treatments indicated that there was no significant difference from the LD(50) value of the susceptible reference strain. The results are a first indication that application technology/insecticide reaction may affect the rapidity of resistance development in certain pest/plant scenarios, but field studies are needed to confirm this conclusion. 相似文献
3.
Flufenoxuron is an acylurea insecticide which inhibits chitin synthesis. Its uptake, excretion and metabolism in larvae of Spodoptera littoralis have been measured. Larvae fed on a leaf disc sprayed at an application rate equivalent to 50 g ha?1rapidly absorbed a maximum of 20% of ingested flufenoxuron, the remainder being voided in the frass. Excretion of absorbed compound was slow, with a 50% excretion time of approximately 20 h. Flufenoxuron was equally effective as an insecticide by topical application or ingestion. Following topical application, a total of 11% of the dose had penetrated into the body within 24 h. Of this 11%, half had travelled to the gut so that the rest of the body tissues contained no more than 6% of the toxicant dose. The concentration of flufenoxuron in the body tissues was maintained for at least 24 h, the surface residue probably acting as a reservoir. The compound was metabolically quite stable. Following topical application the 6 % of the applied dose which was absorbed into the body was still 92% as unchanged flufenoxuron 24 h after application. The enhanced toxicity of flufenoxuron to S. littoralis compared with earlier acylureas can probably be attributed to its slower metabolism and reduced excretion. 相似文献
4.
Ultra-low volume (ULV) spray bioassays at droplet densities (20–80 drops cm?2) corresponding to field deposits were used to determine the efficacy of two acylurea insect growth regulators, teflubenzuron (‘Nomolt®’) and flufenoxuron (‘Cascade®’) against three different age groups of third-instar Spodoptera exempta and Spodoptera littoralis larvae. While no response (mortality and/or abnormal moulting with impaired ability to feed) was observed with one-day-old larvae until 48 h after treatment, two-day-old larvae showed some response (27–93% abnormal moulting and 3–10% mortality when larvae were sprayed; 80–100% abnormal moulting and up to 83% mortality when larvae and plants were sprayed) after 24 h and up to 100% mortality at 120 h. Studies with non-feeding, pre-moult larvae showed that cuticular uptake of acylureas was sufficient to affect an appreciable proportion of larvae after 24 h, with up to 100% mortality at 120 h. More detailed studies with pre-moult S. littoralis larvae showed that, while a few larvae which moulted between 1 and 5 h after treatment showed abnormal moulting to the L4 stage, a much larger proportion of larvae which moulted 6–20 h after treatment were affected. Previous studies with S. exempta have shown that different larval instars show similar levels of sensitivity to acylureas. The present work suggests that, while the age distribution within instars has little influence on the end-point mortality of acylureas, it can profoundly modify the speed of response, the majority of larvae within an instar being effectively controlled at the moult following treatment. The significance of these observations in relation to the crop protection activity of acylureas against field populations of Spodoptera larvae is discussed. 相似文献
5.
The pick-up of pesticide by lepidopterous larvae walking across ultra-low volume pesticide treated surfaces has been stochastically modelled and simulated using a computer. The duration of the random walk was considered as a series of small, discrete time intervals during which the mode of pick-up varied, depending upon the behavioural state (resting, feeding or walking) of a larva. Estimates can be obtained for the total mass picked up by the larva (gross) and the net amount eliminated, the internal exposure to insecticide, and the proportion of insects knocked down and killed with increasing time. The results of the simulation of Spodoptera littoralis larvae confined to permethrin-treated surfaces were in agreement with experimental data, and suggested that a high pick-up of insecticidal droplets resulted from adhesion of the pesticide to larvae following encounter. However, the availability of drops at the leaf surface decreased with time, probably as a result of droplet spread and subsequent uptake by the cuticular waxes. Droplet stability on leaf surfaces may be related to droplet size. 相似文献
6.
Mohsen A. El-Guindy Mostafa M. Abdel-Sattar Abdel Rahman M. El-Refai 《Pest management science》1983,14(3):246-252
The joint action of insecticides, or of mixtures of insect growth regulators and insecticides, on the susceptible (S) strain and diflubenzuron-resistant (Rd) strain of the cotton leafworm Spodoptera littoralis Boisd. was investigated. The joint action of the insecticides and/or insect growth regulator mixtures was determined by mixing them in proportion to their activity equivalents at the LD25 or ED25 levels. A total of 15 mixtures of two synthetic pyrethroids, two organophosphorus, one carbamate and one organochlorine insecticides, were applied to the fourth-instar larvae of the S and Rd strains. The insecticide mixtures cypermethrin/methomyl and cypermethrin/endrin exhibited high and moderate levels of synergism on the S strain, respectively. However the mixtures chlorpyrifos/methomyl, phosfolan/methomyl, and phosfolan/endrin produced antagonism, while the other mixtures showed varying levels of additive effects. The response of the fourth-instar larvae of the S strain, to the joint action of diflubenzuron/juvenoid, diflubenzuron/insecticide, or insecticide/juvenoid mixtures, revealed that diflubenzuron produced high levels of synergism when combined with methoprene and progressively less with fenvalerate, methomyl and cypermethrin. On the other hand, the mixture diflubenzuron/triprene was antagonistic. Fenvalerate with the two juvenoids produced synergism while methomyl showed an additive effect with methoprene. However, the mixtures cypermethrin/methoprene, cypermethrin/triprene and methomyl/triprene produced antagonism. The mixtures that produced potentiation on the fourth-instar larvae of the S strain lost their high potency when tested against the Rd strain. The results also indicated that insecticide/juvenoid mixtures, when applied on 2-day-old pupae of the S strain, were synergistic, except in the case of cypermethrin/methoprene and methomyl/triprene mixtures, for which additive effects were observed. When the mixtures that had synergistic effects on the S strain were tested on the Rd strain, the results revealed that their synergistic effects were apparently reduced. This was attributed to the fact that the generalised levels of tolerance in the Rd strain towards various compounds may have influenced the several defence mechanisms to act against the synergistic action of the chemical mixtures. 相似文献
7.
海灰翅夜蛾Spodoptera littoralis是全球范围内热带和亚热带地区的重大农业入侵害虫,也是我国进境检疫性有害生物,其对欧洲、非洲和亚洲地区的农作物产生严重危害,并造成大量的经济损失。本研究基于海灰翅夜蛾的世界617个分布记录和8个生物气候因子,利用优化的MaxEnt模型预测其在全球和我国的潜在地理分布区,并进一步分析其在我国的传入、定殖和扩散风险。结果表明:气温是影响海灰翅夜蛾潜在地理分布的主导生物气候因子。其在全球的潜在地理分布区主要位于北美洲南部、南美洲、非洲、亚洲西部和东部、欧洲南部和大洋洲,在我国主要位于东部和南部地区。在我国,其潜在地理分布区范围内有着较多的贸易口岸和广泛的寄主分布,增大了其传入我国的风险,并为其定殖和扩散提供了有利的条件。建议海关口岸、农林行业部门加强对海灰翅夜蛾的检疫、调查和监测工作,严防海灰翅夜蛾传入。 相似文献
8.
Christian Kjaer Niels Elmegaard Jrgen A. Axelsen Per N. Andersen Nanna Seidelin 《Pest management science》1998,52(4):361-371
Direct topical impact of an insecticide spray on a population of a non-target leaf-eating beetle, Gastrophysa polygoni, was studied, and the relative importance of phenology, instar susceptibility and instar specific exposure was evaluated. Two insecticides, cypermethrin and dimethoate, were investigated. In the laboratory, topical toxicity to eggs, second-instar larvae and adults was recorded in dose-response experiments. The spatial distribution of larvae and eggs were measured in the field. Deposition of insecticide onto eggs, second-instar larvae and adult specimens was measured at different positions within the crop canopy by use of a dye tracer technique. A temperature-driven population model was constructed to simulate population development of all life stages in the field. The model was based on laboratory measures of growth and development at various temperatures. Mortality due to direct insecticide exposure was calculated as a function of population demography, spatial distribution of individuals, spatial deposition of the insecticide, and stage-specific susceptibility. Cypermethrin had the greatest impact, reducing population size by 19–32%. The life stages most sensitive to cypermethrin were the larval instars. As the population developed from eggs to larvae and imagines, the impact of one spraying first increased and then decreased according to the proportion of larvae in the population. Dimethoate had less effect on the population, i.e. 1·9–7·6% reduction. Dimethoate was most toxic to the egg stage, and consequently the effect on the population decreased as the proportion of eggs decreased due to hatching. The direct effect of insecticide spraying was significantly affected by all three factors investigated, i.e. phenology, life stage susceptibility and stage-specific exposure. The latter factor is composed of both spray flux at various spatial positions in the canopy and the ability of different life stages to retain spray droplets. © 1998 SCI 相似文献
9.
Keng-Hong Tan 《Pest management science》1981,12(6):619-626
An antifeeding (feeding deterrent) response by first- and fifth-instar larvae of Pieris brassicae was shown to be significant at sub-lethal levels of cypermethrin and permethrin, using leaf-discs treated with the pyrethroids by dipping. Permethrin was slightly more effective than cypermethrin both as an insecticide and as an antifeedant against fifth-instar larvae, but the reverse order of effectiveness was observed, with a marked difference in mortality, against newly-hatched larvae. When 1-day-old fifth-instar larvae were continuously given leaf discs treated with a solution of the pyrethroid (1 mg litre?1), up to the time of pupation, both cypermethrin and permethrin induced a significant extension of the larval period, with a reduction in the maximum larval and pupal weights, as well as a reduction in the total leaf-area consumed. However, at higher levels of both pyrethroids, lowering the temperature induced some irritancy, as indicated by regurgitation and frequent uncoordinated wriggling movements of the fifth-instar larvae. The advantages of cypermethrin as a protectant and as an antifeedant over permethrin are discussed. 相似文献
10.
Baculoviruses can alter the development and physiological status of their insect hosts. In the present study, two Spodoptera littoralis nucleopolyhedroviruses (SpliNPV-TR1 and SpliNPV-M2) were examined in terms of their effect on the stage development of S. littoralis at two doses/concentrations by inoculation of neonates or third instars. Both isolates had an acute pathological effect on neonates. However, larval development was prolonged (6-36 h) by either infection when neonates were infected. When third-instar larvae were inoculated, no mortality occurred until insects moulted to later stages. When the isolates were compared, more mortality occurred in the fifth instar with SpliNPV-M2 than with SpliNPV-TR1, whereas less mortality occurred in the sixth instar with SpliNPV-M2 than with SpliNPV-TR1. However, none of the isolates demonstrated a dose effect. Larvae died at 108-198 h post-inoculation at all infections, and none of them pupated. Larval development was retarded (0-126 h) for each stage when third-instar larvae were inoculated. Consequently, SpliNPV-M2 would be a more effective control agent than SpliNPV-TR1 for control of S. littoralis since it kills larvae at earlier developmental stages. 相似文献
11.
Martyn G. Ford Richard C. Reay David Pert Peggy E. Ellis Lawrence J. McVeigh 《Pest management science》1977,8(3):203-210
The contact toxicity of ten pyrethroids to larvae of Spodoptera littoralis (Boisd.) was investigated by topical application, and symptoms observed for different combinations of dose and time. Several compounds were sufficiently active to suggest that they may prove useful for the control of populations in the field. Structure and activity are discussed with particular reference to time-dose-response relationships: speed of action is related to both chemical structure and applied dose. 相似文献
12.
A simple two-compartment model, introducing a parameter (λ) to represent the relative thermodynamic capacities of the compartments, is described for the movement of insecticide from the insect surface (compartment 1) to the region within the insect (compartment 2); loss from compartment 2 is considered. First-order rate constants are assumed for penetration (kp) and elimination (ke). The differential equations describing the rates of change of insecticide in each compartment after application of insecticide are solved analytically, and terms describing (a) the integrals of mass of compound in each compartment with time after application, (b) time to a maximum level of insecticide in compartment 2, and (c) distribution and loss of compound from the system with time, are derived. Methods for estimating the three parameters of the model (kp, ke and λ) are presented. Further properties of the model with possible toxicological significance have been identified; in particular, the total integral for material in compartment 2 depends only on the elimination rate constant and the applied dose. 相似文献
13.
The two insecticidal benzoylurea compounds, diflubenzuron and chlorfluazuron, show large differences in their toxicity against the larvae of insects like the tobacco budworm, Heliothis virescens, or the Egyptian cotton leafworm, Spodoptera littoralis, chlorfluazuron being about 100 times more toxic. This difference is due mainly to a much faster metabolism of diflubenzuron. Its half-life within the larvae is about 5 h, compared to about 50 h for chlorfluazuron. Chlorfluazuron is also the much better ovicide of the two, following injection of the compounds into the females of H. virescens. Again the difference in the rate of metabolism is the main cause. The rate of excretion of the parent benzoylureas is relatively low, but their metabolites are excreted very quickly. 相似文献
14.
Photo-oxidation of the neem limonoids nimbin and salannin with UV light in the presence of oxygen gives two isomeric lactone products per limonoid, nimbinolide and isonimbinolide, and salanninolide and isosalanninolide, respectively. When compared in insect tests with the important limonoids of neem seeds, azadirachtin, nimbin and salannin, isonimbinolide and isosalanninolide show activity greater than that of nimbin or salannin and in some respects show activity approaching that of azadirachtin. The photo-oxidation products were tested for anti-feedant activity and toxicity against larvae of three species of Lepidoptera, Spodoptera littoralis (Boisd), Spodoptera frugiperda (FE Smith) and Helicoverpa armigera (Hübner) and nymphs of the locusts Schistocerca gregaria (Forsk?l) and Locusta migratoria (L). 相似文献
15.
BACKGROUND: To reduce rates of synthetic insecticide applications, natural product alternatives and synergists are needed. A study has been made of the toxicity of ethanolic senescent leaf extracts (SLEs) of Jatropha gossypifolia and Melia azedarach on larvae of the noctuid pest Spodoptera frugiperda. Their effects as syngergists and inhibitors of several enzyme activities are also reported. RESULTS: When added to the diet, M. azedarach SLE showed lower toxicity than J. gossypifolia SLE. However, after 2 weeks on the diet, the M. azedarach SLE proved to be lethal to 100% of the larval population. Artificial diets with both SLEs have an antifeedant effect on armyworm larvae. Acute toxicity after topical application in a dipping assay was relatively low for both J. gossypifolia and M. azedarach SLEs (LC50 of 2.6 and 1.4 g L?1, respectively, after 24 h). However, mixtures of the SLEs of M. azedarach and J. gossypifolia had a strong synergistic effect with cypermethrin. Synergism was higher with the J. gossypifolia SLE, perhaps because it contains several natural products with a methylenedioxyphenyl moiety. Both extracts inhibited P450, general esterase and acetylcholinesterase activities in vitro and in vivo. CONCLUSION: Both J. gossypifolia and M. azedarach SLEs are antifeedants to armyworm larvae when present in the food, and also have a synergistic effect with cypermethrin in topical assays. Although the synergistic effect is less than with piperonyl butoxide, both SLEs have some inhibitor activity against detoxification enzymes and acetylcholinesterase. Thus J. gossypifolia and M. azedarach SLEs may be considered as ecofriendly approaches for the control of S. frugiperda in order to reduce cypermethrin usage. Copyright © 2012 Society of Chemical Industry 相似文献
16.
Wally M. Blaney Monique S. J. Simmonds Steven V. Ley James C. Anderson Stephen C. Smith Anthony Wood 《Pest management science》1994,40(2):169-173
Decalin and dihydrofuranacetal fragments related to those in azadirachtin exhibited antifeedant activity against larvae of the African leaf-worm Spodoptera littoralis. Boisd. All the decalin fragments tested were methoxy (C11) derivatives of azadirachtin. The most active decalin fragment had a ketone substitution at C7. Overall, the compounds were more active when tested in combinations of one decalin fragment and one dihydrofuranacetal fragment than when tested singly. Although some of these combinations did show significant levels of antifeedant activity, sometimes coupled with a synergistic effect, they were not as active as either azadirachtin or dihydroazadirachtin. 相似文献
17.
18.
K. ShankarganeshSuresh Walia Swaran Dhingra B. SubrahmanyamS. Ramesh Babu 《Pesticide biochemistry and physiology》2012,102(1):86-90
Resistance in Spodoptera litura (Fabricius) has been attributed to enhanced detoxification of insecticides by increased levels of esterases, oxidases and/or glutathione S-transferases. Enzyme inhibiting insecticide synergists can be employed to counter increased levels of such enzymes in S. litura. Dihydrodillapiole induced synergism of pyrethroid toxicity was examined in the laboratory-reared third instar larval population of S. litura collected in Delhi (susceptible), and Guntur (resistant) region of Andhra Pradesh, India. The Guntur population was found to be 7.04 and 10.19 times resistant to cypermethrin and lambdacyhalothrin, respectively. The activity of cypermethrin, lambdacyhalothrin and profenophos against susceptible and resistance populations of S. litura, was gradually increased when used along with a plant-derived insecticide synergist dihydrodillapiole. The α-naphthyl acetate hydrolysable esterase activity in Delhi population was less as compared to the Guntur population. Resistance associated esterases in Delhi population were inhibited by pre-treatment with dihydrodillapiole. The esterase level in insect was instantly reduced initially, sustained for about 3 h and equilibrated at 4 h post treatment. The esterase activity of Guntur population was increased to 1.28 μmoles/mg/min at 2 h post treatment and subsequently reduced to lower than 0.70 μmoles at 4-12 h post treatment. The variation in esterase activity is suggestive of its homeostatic regulation in test populations. Dihydrodillapiole thus caused significant reduction of resistance in S. litura to cypermethrin, lambda cyhalothrin and profenophos. 相似文献
19.
Factors determining theeffectiveness of permethrinat ultra-low volume against larvae of Spodoptera littoralis (Boisd.) were investigated using a modified Potter tower. Higher mass application rates for 95 % control were necessary when permethrin was used as a residual deposit compared with direct contact plus residual deposit action. Mortality was increased by adjusting droplet density, although the optimum number of drops/ unit area depended on the mass application rate. When mass application rate and droplet density were held constant, mortality increased inversely with the mean droplet diameter. The implications of these results for field applications are discussed. 相似文献
20.
Leaf-dip bioassays with commercial pesticide formulations were used throughout this study. Third-instar larvae of Spodoptera littoralis were bioassayed for comparative purposes. The end-point mortality against the third-instar larvae (L3) of two Spodoptera exempta populations (Bangor and Gent) and a S. littoralis population (NRI) was found to occur at 72 h and 120 h for neuroactive compounds (carbamate, organophosphate and pyrethroid insecticides) and acylureas respectively. Overall, the acylureas were found to be more toxic (up to 240-fold at the LC50 level) than the most active of the neuroactive compounds tested, the pyrethroids. This difference was greatest against S. littoralis, which was markedly less sensitive to the latter compounds. The Gent population of S. exempta was also found to be significantly less sensitive (7-folt at LC50) to the pyrethroid, lambda-cyhalothrin, than the Bangor strain. Two of the most toxic neuroactive and non-neuroactive compounds, the pyrethroid, cypermethrin and the acylurea, teflubenzuron were chosen for further studies with S. exempta (Gent) and S. littoralis. Relatively little difference in the toxicity of teflubenzuron was observed against different larval instars (L2, L3 and L4) of S. exempta when compared with cypermethrin. Choice-chamber-experiments on feeding behaviour showed that when L3 larvae of either species were exposed to both treated and untreated leaves. teflubenzuron had no significant effect on larval distribution, the percentage of each leaf disc consumed or larval weight gain when compared with untreated controls. In contrast, a marked dose-dependent repellent response to cypermethrin-treated leaves was shown by S. littralis after 6 h. together with corresponding reductions in the percentage of the treated leaf consumed and in larval weight gain. Similarly, when L3 S. exempta were exposed only to pesticidetreated leaves, cypermethrin significantly reduced premoult larval weight gain, whereas tefiubenzuron had no premoult effect. However, following exposure of L3 S. exempta and S. littoralis to teflubenzuron for 48 h prior to moulting, L4 which survived the moult showed reduced weight gain compared with controls, the reduction being more pronounced on maize than on artificial diet. This antifeedant effect increased in proportion to the concentration of teflubenzuron originally administered. The effect of exposure time to teflubenzuron was examined by placing L3 S. exempta and S. littoralis on leaves treated with the approximate LC50 or LC95 dose for 6–48 h. The exposure time required to produce appreciable mortality (relative to LC values) within the treated population was found to be 24 h. 相似文献