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1.
为建立高效液相色谱法测定无核白葡萄果实中赤霉素含量（残留）的方法,样品采用冷甲醇萃取,超声波提取,离心后上清液经0.45 um 滤膜过滤,滤液用 HPLC 紫外检测器测定。色谱条件为以 A（0.05%磷酸水溶液）/B（乙腈）（A / B：80%/20%）为流动相,ZORBAX Extend C18柱,柱温为30℃,紫外检测器波长210 nm。结果表明：该法线性良好,相关系数r＞0.999,精密度在0.86%～3.43%范围之内,平均回收率89.83%。本方法快速、可靠、灵敏,可为测定无核白葡萄果实中赤霉素含量提供参考。 相似文献
2.
‘丰香’草莓果实发育过程中抗氧化物质与活性氧代谢研究 总被引：2，自引：1，他引：1
以草莓栽培品种‘丰香’（Fragaria × ananassa‘Toyonaka’）为试材,探讨其果实发育过程中抗氧化物质与活性氧的变化。结果表明,在果实发育过程中,果实产生的超氧阴离子（）逐渐增加,超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）活性,维生素E、总酚、类黄酮含量以及抗氧化能力显著降低,MDA含量增加。其中,维生素E、总酚和类黄酮是草莓果实发育过程中主要的抗氧化物质,与草莓果实的抗氧化能力密切相关,并在抑制和H2O2的产生方面起着重要作用,而花青素和抗坏血酸在草莓发育过程中所起的抗氧化作用较小。 相似文献
3.
在测定还原型维生素C时,一些含花青素的蔬菜,如萝卜、茄子、莴苣、绿菜花的某些品种,由于酸提取液呈红色,无法应用常规的2,6-二氯酚靛酚滴定法。有色提取液中维生素C的测定,可用电位滴定法、萃取比色法或汞测法。萃取比色法[1][2]不需要特殊设备,操作比较简单。这里拟定的二甲苯萃取比色法,经反复试验表明,测定结果与滴定法基本一致,方法的准确性和重现性较好。 一、原理 向一定量提取液加入过量的2,6-二氯酚靛酚染料溶液,与维生素C作用后,多余的染料用二甲苯萃取,比色。待测液中维生素C的含量与二甲苯萃取液的颜色呈线性负相关,即待测液… 相似文献
4.
草莓ABA 的快速提取方法及超高效液相色谱分析 总被引：1，自引：0，他引：1
采用甲醇︰乙酸乙酯︰甲酸 = 50︰50︰1 的混合液作为提取溶剂,可快速从草莓中提取ABA,
整个提取过程可在0.5 h 内完成,大大提高了ABA 提取效率。建立了一套适合ABA 分析的超高效液相色
谱分析体系,其流动相为甲醇︰乙腈︰0.1%甲酸 ＝ 20︰10︰70,流速0.3 mL · min-1,检测波长265 nm,
柱温30 ℃,进样10 μL。利用建立的快速提取法及超高效液相色谱分析方法,测定了不同发育阶段草莓
果实（青果、白果、粉红果和红果）的ABA 含量,发现其呈上升趋势,至红果期ABA 含量达到最高。 相似文献
5.
茄子果实中α-茄碱提取方法的研究 总被引：1，自引：0，他引：1
以茄子品种'辽茄七号'果实为试验材料,设计了单因素试验和正交试验,采用高效液相色谱法对不同提取方式、不同超声波提取条件下茄子果皮和果肉中α-茄碱的含量进行测定。结果表明：采用超声波提取法时,茄子果实中α-茄碱的提取量最高,提取前后α-茄碱性质保持不变。优化的超声波提取条件为：以料液比为1:10的70%甲醇为提取剂,在50 ℃下提取60 min。测得果皮中α-茄碱平均提取量为0.107 mg·g-1,相对标准偏差 (RSD) 为1.80%（n=5）,果肉中α-茄碱平均提取量为0.620 mg·g-1,RSD为1.29%（n=5）,平均回收率为98.0%,RSD为1.23%（n=3）,说明该方法的提取率和精密度高、稳定性好,合理可行。 相似文献
6.
海棠果实中脱落酸的高效液相色谱测定 总被引：1，自引：0，他引：1
以‘草莓果冻’海棠为试材,采用高效液相色谱结合固相萃取法建立了一种用于海棠果实中脱落酸(ABA)含量检测的方法。通过优化前处理及色谱条件,最终确定取样量为3g,用80%预冷甲醇浸提12h,经不溶性PVPP吸附酚类等杂质,石油醚脱色,乙酸乙酯萃取,C18固相萃取小柱进一步纯化,再经甲醇∶1.0%乙酸溶液(45∶55,v/v),流速为1.0mL·min~(-1)进行洗脱,C18色谱柱分离,进样体积20μL,检测波长为254nm,柱温45℃。结果表明:该方法在0.01~0.50μg·mL~(-1)范围内线性关系良好,R~2=0.999 8,3个浓度水平下平均回收率均大于70%。采用该方法对‘草莓果冻’海棠半同胞子代中14个单株落果初期果实进行了测定,发现果实中脱落酸含量最低为0.026 9μg·g~(-1),最高含量为0.357 9μg·g~(-1),差别较大,该浓度与已有报道中的测定结果处于同一数量级水平。该方法的建立对于海棠的选育和生长发育进一步研究具有重要意义。 相似文献
7.
自然五倍体野生草莓果实风味物质分析 总被引：2，自引：0，他引：2
以中国自然五倍体野生草莓黑1与黑6的成熟果实为试材,采用顶空固相微萃取（HS-SPME）、气相色谱—质谱（GC–MS）及高效液相色谱（HPLC）技术,分析果实香气、糖、酸成分。结果表明,自然五倍体野生草莓含11类92种挥发性成分,相对含量大于1%的成分有5类共22种,其中乙酸乙酯、丁酸乙酯、3–甲基丁酸乙酯、己酸乙酯、乙酸桃金娘烯醇酯、2–戊酮、甲基壬基甲酮、乙醇、2–庚醇、1–己醇、（E）–2–己烯–1–醇、己醛、（E）–2–己烯醛、4–甲氧基–2 ,5–二甲基–3（2H）–呋喃酮是主要的香气物质,与东方草莓黑10（4x）、东北草莓吉12（2x）相比,3–甲基丁酸乙酯为自然五倍体试材所特有,而4–甲氧基–2 ,5–二甲基–3（2H）–呋喃酮、2–戊酮、2–庚醇、1–己醇、（E）–2–己烯–1–醇的相对含量较高;自然五倍体野生草莓的主要糖成分为果糖,其次为葡萄糖,蔗糖最少,山梨醇在两份野生资源中差异较大;苹果酸为自然五倍体野生草莓的主要酸成分,其含量占总酸含量的86%以上。 相似文献
8.
为了探究辣椒种质资源果色与呈色物质之间的关系，利用色差仪测定辣椒果实色彩空间（CIE L*a*b*）值，采用分光光度计测定叶绿素a和叶绿素b含量，利用液相色谱法（HPLC）测定辣椒红素、玉米黄质、β–胡萝卜素和6种花青素（飞燕草素、矢车菊素、锦葵素、芍药花素、天竺葵素、牵牛花素）的含量，并基于Python语言进行辣椒果色可视化分析，利用主成分分析（PCA）、正交偏最小二乘法（OPLS）、聚类分析和集成推进树（ABT）对辣椒果色和呈色物质关系进行分析。结果表明：63份辣椒种质被划分成A、B两个颜色群体，其中A偏向黄绿色，B偏向紫黑色；辣椒果皮的CIE L*a*b*值与呈色物质含量之间有相关关系，玉米黄质、叶绿素a、飞燕草素对果色CIE L*a*b*值贡献率较大；在相同类胡萝卜素含量下，叶绿素、飞燕草素含量较低时，果实呈黄绿色，飞燕草素含量较高时呈紫黑色。 相似文献
9.
10.
1树种特征特性蓝莓又名蓝莓果、蓝浆果和越枯。蓝莓属杜鹃花科越桔属．为多年生灌木或小乔木植物。株高O．5-1．8m．叶互生。前面有茸毛，总状花序侧生或顶生：花单生或双生在叶腋间；花冠钟状或管状，4—5裂片．多为白色或粉红色；管状花托，花托与子房贴生；8-20个雄蕊：果实为圆形蓝色浆果，被白色果粉，平均单果重0．5，2．5g．最大的可达3．5．5g；籽细微，果肉细腻，具有独特适口的酸甜香味．并富含蛋白质、碳水化合物、维生素A、B、C和E；并含有铁、锌、钙、锰、钾等微量元素：还含有花青素、花红素、花青甙和熊果甙等无机元素： 相似文献
11.
Seven wild and ten cultivated blackberries (Arapaho, Bartin, Black Satin, Bursa 1, Bursa 2, Cherokee, Chester, Jumbo, Navaho, and Ness), and six lowbush (Vaccinium arctostaphylos) and four highbush (Vaccinium corymbosum) blueberries fruits (Ivanhoe, Jersey, Northland, and Rekord) were analyzed for total anthocyanins, total phenolics, and antioxidant activity as ferric reducing antioxidant power (FRAP) in this study. The respective ranges of total anthocyanin and total phenolic contents of the tested samples were: blackberries, 0.95–1.97 and 1.73–3.79 mg g−1 and blueberries, 0.18–2.94 and 0.77–5.42 mg g−1. FRAP values varied from 35.05 to 70.41 μmol g−1 for blackberries, 7.41 to 57.92 μmol g−1 for blueberries. Wild blackberries had the highest FRAP values while wild blueberries had the highest total phenolic and total anthocyanin contents. A linear relationship was observed between FRAP values and total phenolics for blueberries (r = 0.981). The anthocyanin pigments in samples were isolated and characterized by high-performance liquid chromatography (HPLC) with UV–visible detection. Cyn-3-glu was the predominant anthocyanin in all blackberry fruits. 相似文献
12.
Stéphanie Guillotin Philippe Sanoner 《The Journal of Horticultural Science and Biotechnology》2013,88(6):96-99
SummaryThe colour of anthocyanins can be enhanced by planar chemical complex formation, or stabilised by the formation of adducts. We investigated these properties using fruit extracts. Delphinidine and its glucoside, myrtilline, at 0.1 mM were mixed with 10 mM caffeoylquinic acid (CQA; chlorogenic acid) or 6 mM ferulic acid (FeA). In a second experiment CQA, ferulic acid, and a CQA-rich apple extract were added in variable concentrations to a blackcurrant pigment preparation and incubated at 37°C in 0.02 M citrate buffer at pH 1.5, pH 3.0, or pH 4.5. The colour change was followed by measuring the absorbance at 540 nm and the composition of the solutions was assayed by HPLC using diode array detection. Deglycosylation of anthocyanins leads to a loss of colour and chemical stability. It is therefore of major importance that the enzyme preparations used during juice extraction be devoid of glycosidases. Addition of phenolic acids to an anthocyanin solution increases and stabilises the colour, which is accompanied by an acceleration of the loss of the native anthocyanins and the formation of coloured adducts, presumably based on the pyranoanthocyanin structure. The glycosyl residue in anthocyanins is necessary for colour stabilisation, which appears to be due to the formation of new pigments. Caffeoylquinic acid appeared to be more efficient than ferulic acid, and a CQA-rich extract from apple also resulted in stabilisation of the colour of a blackcurrant extract. 相似文献
13.
以10个不同花色的花毛茛（Ranunculus asiaticus）和4个不同花色的银莲花（Anemone cathayensis）为材料,采用目视测色法,RHSCC 比色卡比色法,色差仪（CR-400）测定花瓣的花色表型,利用双光束紫外—可见光分光光度计（TU-1901）、高效液相色谱—电喷雾离子化—质谱连用技术（HPLC–ESI–MS）对花瓣中花青素苷的成分及结构进行测定,运用多元线性回归方法分析花色与花青素苷组成之间的关系。结果表明：8个积累花青素苷的花毛茛品种花瓣中检测到5种花青素苷元,即天竺葵素、矢车菊素、飞燕草素、芍药素和锦葵素,这些花青素苷元进一步修饰形成了15种花青素苷。红色系和粉色系品种以芍药素和矢车菊素为主要成分,黄色系品种以天竺葵素或类胡萝卜素为主要成分;经分析矢车菊素苷和天竺葵素苷含量均与亮度L*值呈显著正相关,其中天竺葵素苷的积累对花瓣亮度的贡献更大。银莲花4个品种花瓣中共检测出6种花青素苷元,分别为矮牵牛素、天竺葵素、矢车菊素、飞燕草素、芍药素和锦葵素,它们通过不同糖基化和酰基化修饰形成了20种花青素苷,呈现出丰富的花青素苷多样性。 相似文献
14.
A method used for anthocyanin measurement in cranberry resulted in a problem of interference by carotene in purple carrot extracts. We developed a method suitable for a purple color carrot breeding program. The new method includes a three step process: (1) extraction of anthocyanins using a small amount of acidified ethanol (95% ethanol in 1.5 N HCl); (2) removal of carotenoids present in the extract; and (3) measurement of total anthocyanins with a spectrophotometer. The purple colored carrots analyzed contained around 38–98 mg anthocyanin per 100 g fresh weight. The new method can be efficiently used in a breeding program for the development of purple colored carrots for nutritional benefits and a source of a natural pigment. 相似文献
15.
杨梅花色苷提取工艺条件的响应面分析及其抗氧化活性 总被引：2，自引：0，他引：2
为提高杨梅资源的利用和活性成分的开发,运用四元二次回归旋转组合设计法及分光光度法探讨了杨梅鲜果花色苷的最佳提取工艺及杨梅花色苷粗提物清除DPPH自由基的效果。结果表明,曲面回归方程与实验结果拟合性好,优化工艺为提取温度35.34℃,液料比(v/w)11.02∶1,乙醇体积分数为68.09%,pH值2.72。在该条件下,杨梅花色苷的提取率为94.33%。将杨梅花色苷提取液通过大孔树脂进行分离,所制备的杨梅花色苷粗提物其清除DPPH自由基的效果优于EGCG,其IC50为0.1026mg·L-1。研究结果表明杨梅粗提物具有较强的抗氧化活性,其中花色苷等酚类物质可能为杨梅中重要的抗氧化成分。 相似文献
16.
Specific components of cranberry fruit are being associated with human health attributes, such as maintenance of urinary tract health and antioxidant status. Some of the chemical properties and antioxidant capacity of cranberry (Vaccinium macrocarpon Ait. cv. Pilgrim) fruits were investigated at light green, blush, light red and dark red maturation stages. Fruit total phenolics, total monomeric anthocyanins, soluble solids, titratable acidity and individual organic acids were examined. Antioxidant capacity of fruits were determined by both the ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays. The fruit color was measured using a portable chromameter. A converse relationship was found between total phenolics and anthocyanin as fruits mature. Total phenolic concentration was declined from 7990 to 4745 mg GAE/kg fw, while total monomeric anthocyanin content was increased from 0.8 to 111.0 mg/kg fw from green to dark red stage. Brix was increased from 6 to 9.3% as well. The main organic acid was citric acid determined by the HPLC method. The antioxidant capacity of cranberries decreased to light red stage; when a fruit accumulates more anthocyanin the activity increased again in both FRAP and TEAC methods. Averaged antioxidant capacity measured was 12.61 and 17.48 mmol TE/kg fw by FRAP and TEAC methods. 相似文献
17.
草莓果实发育过程中花色苷和黄酮醇类物质的形成 总被引：2，自引：0，他引：2
以‘赛娃’草莓为试材,研究了果实中花色苷和类黄酮类物质的形成机制。在‘赛娃’果实中共检测到12种花色苷和7种黄酮醇类物质。幼果期(盛花后15～25 d)果实内没有花色苷的积累,但黄酮醇类物质大量积累;随着果实成熟,花色苷含量逐渐增加,黄酮醇类物质含量降低;遮光导致花色苷和黄酮醇类物质的积累降低了70％～90％,尤其是花青素糖苷和槲皮素糖苷的含量降低了90％以上,表明遮光抑制草莓花色苷和黄酮醇类物质的积累,且对花青素、槲皮素合成的抑制明显高于花葵素和山奈酚。草莓果实类黄酮类物质代谢在发育前期主要向双氢槲皮素方向合成,果实成熟期主要向花葵素方向合成。 相似文献
18.
Yasuhiro Takahata Yumi KaiMasaru Tanaka Hiroki NakayamaMasaru Yoshinaga 《Scientia Horticulturae》2011
To investigate the effect of differences in anthocyanin composition on radical-scavenging activity in the purple-fleshed sweetpotato storage root, 124 sweetpotato clones with various compositions of anthocyanin were produced by crosses among superior sweetpotato genetic resources, and were analyzed for anthocyanin composition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity (RSA). The highest content of anthocyanin among the clones was twofold higher than the content of cv. Ayamurasaki, which has been released for use in industrial pigment production in Japan. The range of variance in anthocyanin composition was very wide, as the ratio of peak areas of cyanidin-based anthocyanins to total anthocyanin peak areas ranged from 85.7% to 6.0%. The relationship between anthocyanin content and RSA was positively correlated. Correlation coefficients between RSA and the HPLC peak areas of cyanidin-based anthocyanins ranged from 0.606 to 0.687, whereas those of peonidin-based anthocyanins were 0.124–0.271. These results clearly demonstrated that cyanidin-based anthocyanins are closely related to DPPH RSA in sweetpotato storage roots. 相似文献
19.
花青苷生物合成转录调控研究进展 总被引：9，自引：0，他引：9
花青苷生物合成由一系列结构基因编码的酶催化完成,而结构基因的表达受MYB、bHLH
和WD40 这3 类转录因子组成的MBW（MYB-bHLH-WD40）转录复合体的协同调控。花青苷合成的转
录调控研究最先在30 多年前见于玉米、拟南芥和矮牵牛等模式植物,近年来在肉质果实上也取得了重要
进展。在介绍果实花青苷的种类、分布与功能的基础上,回顾了MYB、bHLH、WD40 这3 类转录因子
的基本特性及其在花青苷合成调控中的作用,进而就MBW 转录复合体协同调控果实花青苷生物合成的
机制研究的近年进展进行了综述。 相似文献
20.
Raquel P. F. Guiné Susana Matos Fernando J. Gonçalves Daniela Costa Mateus Mendes 《International Journal of Fruit Science》2018,18(2):199-214
ABSTRACTThe study aimed at evaluating the influence of different production conditions, conservation, and extraction procedures on the total phenolic compounds and antioxidant activity of blueberries by DPPH and ABTS methods. The production factors considered were origin, altitude of the farm location, and age of the bushes. The conservation conditions considered were freezing as opposed to the fresh product. The extraction procedures included two different solvents and two orders of extraction. The data analysis was carried out by training artificial neural networks to model the data and extract information from the model.The results obtained revealed that the type of extract and the order of extraction influenced the concentrations of phenolic compounds as well as the antioxidant activity of the different samples studied. Also the origin of the farms from where the blueberries were harvested significantly influence those properties, showing that the blueberries from Oliveira do Hospital had less phenolic compounds and lower antioxidant activity. Also older bushes at higher altitudes seem to produce berries richer in these properties. Regarding conservation, no influence was observed for phenols but a slight influence could be detected for antioxidant activity. 相似文献