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1.
ABSTRACT

The diffusion properties of sodium chloride in rainbow trout muscle during dry salting at 10°C were investigated. The average initial dry matter content of the samples increased from 27.80 ± 0.10 to 36.01 ± 0.16%, and the average salt content increased to 17.98 ± 0.38 g NaCl/100 g dry matter at the end of the 10-h dry salting process. The analytical solution of Fick’s second law considering one-dimensional diffusion through an infinite slab was used to calculate effective salt diffusion coefficients (Deff). The Deff values in rainbow trout fillets decreased with increasing salting time and ranged from 6.64 × 10?10 to 16.45 × 10?10 m2/s.  相似文献   

2.
Drying experiments were conducted at different temperatures and air flux velocities to determine the proper drying conditions for reducing moisture in commercial fish feed impregnated with probiotic lactic acid bacteria and to assess the effect on bacteria viability over time. At temperatures of 45°C, the drying time was shorter, without the air flux velocity under study having a relevant influence. The drying conditions influenced the viability of the bacteria in the feed; the least loss of viability was obtained with a velocity of 0.8 m s?1 and a temperature of 45°C during 15 min. Using these drying conditions, 5 kg of feed was dried and stored for a month at temperature of 26°C and relative humidity of 75%. The viability of the bacteria and the moisture of the feed were measured every 3 days during the storage period. Loss of viability followed first order kinetics, with a constant k of 0.112 days?1. Thus, the viability of the bacteria in the feed is less than 106 CFU g?1 after 43 days.  相似文献   

3.
From mid-August to mid-September 1998, a dinoflagellate bloom in a shrimp pond near La Paz, Baja California Sur, was studied. Daily samples were taken of phytoplankton, dissolved oxygen, nutrient concentrations, photosynthetic pigments and proteins. The bloom occurred at temperatures of 29.7 to 33.2?°C. High densities of a Scrippsiella-like dinoflagellate (15.9?×?106 cells/L) occurred on the first day of sampling, reaching a peak of 249?×?106 cells/L on Day 13, and declining to 4 to 7?×?106 cells/L by Day 26. Chlorophyll a varied from 8.63 mg/m3 on Day 4 to 141.38 mg/m3 on Day 13. Total nitrogen varied widely (0.2–11.50 μM) while phosphates peaked only once on Day 13 (4.14 μM). Proteins ranged from 523 to 6387 μg/L; the latter value occurred two days after the maximal peak of nitrates and coinciding with the highest level of phosphates. Fertilizers added to the ponds increased nutrient concentration and stimulated phytoplankton growth. In turn, the food supply to cultured shrimp noticeably increased throughout the bloom.  相似文献   

4.
Quantitative and qualitative analyses of bacterial flora associated with pond water, gills, and intestine of polycultured healthy common carp (Cyprinus carpio) and African catfish (Clarias gariepinus) were carried out and identified to species level where possible. Total viable bacterial counts in the pond water ranged from 9.2?±?5.5?×?103 to 6.6?±?5.1?×?104 colony-forming units (cfu)/mL; in the gill filaments of carp and catfish, 3.3?±?3.8?×?106 to 7.9?±?5.6?×?106 and 1.1?±?4.6?×?105 to 2.3?±?5.2?×?106 cfu/g, respectively; and in the intestine of carp and catfish, 1.4?±?2.9?×?1010 to 1.7?±?6.0?×?1011 and 2.7?±?3.4?×?1010 to 1.0?±?4.5?×?1011 cfu/g, respectively. Gram-negative rod-shaped bacteria dominated the populations: 90% in carp, 89% in catfish, 80% in water, and 86% in the total populations. Altogether, 14 bacterial species of 10 genera were identified in total populations. Pond water bacteria had a reflection on the bacterial composition of the gills and intestine of carp and catfish. Aeromonas hydrophila, Shewanella putrefaciens, Vibrio cholerae, Staphylococcus sp., and Vibrio vulnificus appeared as the common bacteria in the populations, where the first three were highly significantly abundant (P?<?0.0001). Moreover, A. hydrophila was the most significantly dominant bacteria (32%; P?<?0.005) among the total populations. Pantoea sp. and Pasteurella pneumotropica were present only in carp and catfish, respectively, but Corynebacterium urealyticum and Micrococcus sp. were present only in pond water.  相似文献   

5.
ABSTRACT

Quantitative and qualitative estimation of bacterial flora present in pond water, sediments, gills, and intestine of healthy common carp Cyprinus carpio cultured in Saudi Arabia were performed and identified to species level where possible. Mean total viable bacterial counts in pond water ranged from 1.2?±?2.9?×?104 to 2.5?±?3.5?×?105 cfu/mL; in sediments, 9.3?±?2.1?×?107 to 2.7?±?3.5?×?109 cfu/g; in gills filaments, 4.3?±?2.9?×?106 to 1.6?±?3.9?×?107 cfu/g; and in intestine, 8.7?±?4.1?×?109 to 5.4?±?3.2?×?1010 cfu/g. Gram-negative rod-shaped bacteria dominated (76%) the populations. In total, 12 bacterial genera and 15 species were identified. Pond water and sediment bacteria had the reflection on bacterial composition of gills and intestine of carp. Intestinal bacteria showed more diversification in contrast to gill bacteria. Aeromonas hydrophila, Shewanella putrefaciens, Vibrio cholerae, Corynebacterium urealyticum, Vibrio vulnificus, Vibrio sp., and Staphylococcus sp. were the common bacteria in all the populations. In pond water and carp intestine, A. hydrophila, S. putrefaciens, V. cholerae, and C. urealyticum were the most dominant bacteria (prevalence ≥ 10%) where pond sediments and the carp gills experienced with more one dominant bacterium V. vulnificus. Only the A. hydrophila covered one fourth (25%) of the total bacterial populations.  相似文献   

6.
Feeding performance (intensity and incidence) of first‐feeding yellowtail kingfish larvae was evaluated under a range of light intensities and algal‐induced turbidities. Larvae were fed with varying degrees of success under all light intensities tested (0–17 μmol s?1 m?2), in both clearwater and greenwater (8 × 104 cells mL?1). There was a consistent trend for feeding performance to increase with larval age and light intensity in both clearwater and greenwater conditions, demonstrating that visual proficiency increases with larval age. Feeding intensity remained low over the first 3 days of first feeding across all light intensities tested. Days 6 and 7 post‐hatch larvae showed considerably higher feeding intensities particularly at 8 and 17 μmol s?1 m?2. This improvement indicates an ontogenetic shift in sensory or locomotor competence. First‐feeding larvae performed equally well in both clearwater and greenwater (8 × 104 cells mL?1) conditions, although at the lowest light intensity tested (0.1 μmol s?1 m?2) feeding performance was noticeably reduced. Feeding performance was severely diminished across all larval ages at an algal cell density of 32 × 104 cells mL?1, demonstrating that for this species algal‐induced turbidities >16 × 104 cells mL?1 adversely affect the ability to capture free‐swimming prey during the first‐feeding window.  相似文献   

7.
This study was undertaken to quantify the relationships between the rate of ammonia excretion by brine shrimp and dry weight (body size) and food density. Measurements of the rate of ammonia excretion of the brine shrimp were made at 25 C in darkness. Under these conditions, the excretion rate of ammonium-nitrogen can be generalized as a function of body weight and food (Nannochloropsis sp.) density. The relationship between the body weight (W) and excretion rate of ammonium nitrogen (EN) is expressed as EN=α Wγ, where α is the metabolic level. The parameter α is dependent on ration, such that α=αo±αr where αo= metabolic level in starvation and αr= the metabolic level which increased with feeding or changed with the food density. In experiments in which the animal starved, the equation above becomes EN=αoWγ, and the values of α and γ were 0.22 and 0.93, respectively. The rate of ammonium excretion rose from 5.42 × 10?4 to 2.28 × 10?1μg N/animal/h as dry weight measured from 1.57 × 10?3? 1.04 mg dry weight per animal. Next, it is convenient to express αr as αr=αfmax= (1 ? 10?kc), where αfmax is the maximum rate of αf at saturated level of food density, C is the mean cell density, and k is a constant defining the rate of change of the metabolic rate with cell density. Therefore, the rate of ammonia excretion by brine shrimp could be expressed as EN=αo [1 ? a(l ? 10?kC)]Wγ, and a =αfmax/αo. The values of the kinetics parameters obtained from experiments where shrimp were fed were a = 2.3 and k = 0.15×10?7. Consequently, the maximum rate of ammonia excretion at a saturated food density is equivalent to 3.3 times the rate of animals that are not fed.  相似文献   

8.
Suitable culture conditions for Rhodomonas sp. Hf-1 strain were examined for high productivity. Hf-1 strain was grown in an incubator for 7 days. The factorial experimental design investigated the following 19 variables: temperature (12, 16, 20, 24 and 28 °C), salinity (7, 14, 21, 28 and 35 psu), light intensity (20, 35, 50, 65 and 80 μmol m?2 s?1), light color (white, red, green and blue lights), and 3 factorial designs of photoperiod (24L:0D, 16L:8D and 12L:12D light:dark cycle). The cell density and specific growth rates (SGR) were analyzed. The best cell growth was observed under the following culture conditions: temperature of 24 °C, salinity of 21 psu, light intensity of 80 μmol m?2 s?1, and white light. In the photoperiod test, the highest cell density of 4.7?×?106 cells ml?1 was obtained at 24L:0D light:dark cycle, and the SGR was 0.57 μ day?1 at this time. We found that the Hf-1 strain was able to be cultured in extremely wide culture conditions. These results are expected to serve as a baseline study for culturing the Hf-1 strain in the laboratory and for its use in aquaculture.  相似文献   

9.
The aim of the study was to evaluate the performance of Litopenaeus vannamei in an intensive photo‐heterotrophic hypersaline system with minimal seawater replacement, and establish relationships between parameters of a stochastic production model and relevant water quality variables. Six experimental 1000 m2 lined ponds were stocked at a density of 120 shrimp m?2 for a 105‐day trial. Salinity increased from 37 to 45 ± 2 g/L, and the water level was maintained with the weekly addition of filtered seawater, equivalent to 1.6% per day. The stochastic model predicted that, at harvest, there is 95% confidence that the system produces between 12.1 and 14.7 t/ha with a mean final individual weight of 13.1 g and a mean survival of 84.2%. Sensitivity analyses showed that dissolved oxygen and individual final weight of shrimp were the main variables influencing yield variance. Nitrogenous compounds were maintained between optimal cultivation levels (NH3–NH4+ = 0.73 ± 0.43 mg/L, N–NO2? = 0.09 ± 0.05 mg/L, N–NO3? = 3.22 ± 0.11 mg/L). Heterotrophic bacteria (6.6 ± 3.4 × 105 CFU/ml) and chlorophyll‐α concentration (108.5 ± 80.2 μg/L) showed a similar development pattern, indicating a strong relationship between bacteria and microalgae during cultivation. Vibrio spp. concentrations were low (1.24 ± 1.42 × 103 CFU/ml). It was shown that the photo‐heterotrophic system could be used in hypersaline conditions, typical of semi‐arid regions, to consistently produce between 12.1 and 14.7 t/ha in 15 weeks.  相似文献   

10.
To determine the optimal salinity, stocking density, and algal density for hatchery culture of the Iwagaki oyster Crassostrea nippona larvae, three experiments with salinities of 14, 18, 22, 26, 30, and 34 practical salinity unit (PSU); stocking densities of 0.5, 1, 2, 4, 8, and 12 larvae ml?1; and algal densities of 10, 20, 40, and 100?×?103 cells ml?1 were designed, which included the developmental stages from newly hatched D-larvae to pediveligers. Results showed that larval growth of C. nippona was the fastest at a salinity of 26 PSU, and when salinity was adjusted to a level that was lower or higher than this salinity, survival and growth rate of larvae declined (P <?0.05), resulting both in a decreased mean shell length and a high mortality. Larval growth decreased significantly with increasing stocking density. Larvae reared at 4 larvae ml?1 had the smallest shell length (198.9 μm) and lowest survival rate (7.9%), whereas larvae reared at 0.5 larvae ml?1 had the largest shell length (245 μm) and highest survival rate (66.3%) on day 13. And the shell length of larvae reared at 0.5 and 1 larvae ml?1 was significantly (P?<?0.05) larger than the values in other treatments, except those reared at 2 larvae ml?1 (P?>?0.05). When feeding the single-algal diet of Isochrysis galbana (clone T-ISO), the shell length of larvae increased markedly as the algal density was increased. Larvae reared at the highest algal density (100?×?103 cells ml?1) had the largest mean shell length; however, under the conditions of our experiment, there was no significant difference (P?>?0.05) in growth and survival rates between the treatments at algal densities of 40?×?103 and 100?×?103 cells ml?1. For a large-scale culture, based on the results of this study, a salinity of 26 PSU, stocking density of 0.5–1 larvae ml?1, and algal density of 40?×?103 cells ml?1 are recommended for an early development of C. nippona.  相似文献   

11.
Sexually mature males (BW?=?1600?±?150 g and TL?=?235?±?30 mm) of northern pike (Esox lucius L.) were randomly selected from a pond to record changes in their sperm quality parameters (spermatozoa morphology, sperm volume, density, and motility parameters) during the spawning season. The morphological and motility parameters changed significantly during the reproductive season with following trends. Only, head width was not changed during the spawning season. The longest spermatozoa and its flagellar length were found at the middle of spawning period (TL?=?38.24?±?0.37 μm and 35.14?±?0.26 μm) and shortest at the beginning of spawning period (TL?=?34.81?±?0.29 μm and 32.53?±?0.18 μm). Other morphological characters were always the lowest at the beginning of spawning period. Sperm volume was changed from 0.33?±?0.3 ml in February, 0.43?±?0.2 ml in March to 0.24?±?0.1 ml in April, and density from 16.2?±?0.2?×?109 spermatozoa ml?1 in February, 19.4?±?0.2?×?109 spermatozoa ml?1 in March to 4.8?±?0.2?×?109 spermatozoa ml?1 in April. Same sperm velocity was observed in all spawning terms at 10 and 20 s after activation. Higher velocity was found at 30 and 40 s after activation in sperm collected at the middle and the end of spawning period. Significantly, higher percentage of motile sperm was observed at 20, 30, and 40 s after activation in sperm sampled at the end of spawning period. This study supports the hypothesis that longer spermatozoa swim faster.  相似文献   

12.
The aim of the present study was to evaluate the effect of acute stress and cortisol injection on oocyte final maturation process in female Nile tilapia (Oreochromis niloticus). Handling followed by a prophylactic treatment (0.3?mL?L?1 H2O2, 5?g?L?1 NaCl solution during 30?min) and an environmental change (transfer from a 2?m3 fibreglass square tank to 50?L aquaria) were used as acute stressors and compared to a single cortisol injection (0.5 or 5?mg?kg?1 body weight). For both acute stress and cortisol injection (0.5?mg?kg?1 body weight), serum cortisol level was significantly increased from 2.3 to 134.1?ng?mL?1 1?h post-stress/injection and returned to a resting basal value 24?h after the stress/injection. In fish injected with 5?mg?kg?1 body weight cortisol, mean serum cortisol level reached a peak up to 2500?ng?mL?1 1?h after injection. 63?% of the females (mean body weight: 242?±?4?g) submitted to the acute stress ovulated within 72?h after the stress. In the same way, cortisol injection (5?mg?kg?1 body weight) at the 10th day of the maturation cycle led to a twofold reduction of the time before ovulation compared to vehicle injected control fish. Relative and total fecundity were significantly decreased in females submitted to an acute stress or cortisol injected at 5?mg?kg?1 body weight, but not fertilization or hatching rates. In conclusion, acute stress and cortisol induction exert both positive and negative effects on the final reproductive process in O. niloticus, and cortisol is the endocrine mediator causing these changes.  相似文献   

13.
On the basis of field data measured during four cruises from April 2011 to January 2012, spatial and seasonal variations of CO2 dynamics and aqueous pCO2 were investigated in the seaweed aquaculture area, Lidao town, China. Results showed that the mean annual concentrations of dissolved inorganic carbon (DIC), HCO3?, CO32?, and CO2 were 2024.79 ± 146.96, 1842.41 ± 132.13, 170.02 ± 42.82, and 12.35 ± 2.52 µmol/L, respectively. There were no significant differences between areas in concentrations of DIC and HCO3? (P > 0.05), while the differences for the concentration of CO2 was very significant (P < 0.01). The mean annual values of aqueous pCO2 and sea‐air CO2 flux were 287.80 ± 37.90 µatm and ?32.71 ± 17.23 mmol/m2/d, respectively. There were very significant differences (P < 0.01) for aqueous pCO2 and sea‐air CO2 flux not only between different areas, but also between different seasons. The buffer factor β indicates that, inside the seaweed area, inorganic carbon dynamics are mainly influenced by photosynthesis and respiration process.  相似文献   

14.
Effects of temperature on food consumption, growth and oxygen consumption were estimated for the freshwater prawn Macrobrachium rosenbergii postlarvae at 23 °C, 28 °C and 33 °C in the laboratory. The results showed that the animal's initial body weight had a close linear relationship with food consumption and growth. Food consumption increased directly with temperature. Consumption rates (C; mg day?1 ind?1 ) of the 28 °C and 33 °C groups were much higher than that of the 23 °C group (P < 0.001), and the 33 °C group's consumption rate was higher than that of the 28 °C group (P < 0.05). The relationship of food consumption with temperature and initial body weight (W; mg) could be described as: C = 0.0679W + 0.185t? 3.17. Growth increased significantly with increased temperature. The relationship among specific growth rate, temperature and initial body weight was as follows: SGR = ?0.110W + 0.213t + 0.176. However, temperature showed no effect on growth efficiency. Oxygen consumption increased significantly with temperature (P < 0.01). The weight‐specific oxygen consumption rates (mg O2 g?1 h?1) at 23 °C, 28 °C and 33 °C were 0.83, 1.16 and 1.49 mg O2 g?1 h?1 for 61.92 mg M. rosenbergii.  相似文献   

15.
To develop a feeding strategy for the Australian freshwater fish silver perch (Bidyanus bidyanus Mitchell), a series of eight experiments was done in 1 m3 cages in an aerated, earthen pond to determine the effects of feeding rate (% body weight) and feeding frequency (no. of feeds day?1) on the growth and feed conversion ratio (FCR) of fingerlings and larger fish under ambient water temperatures over the range 13.8–30.6°C. Fish were fed extruded pellets of a silver perch diet containing 34% digestible protein and 14 MJ kg?1 digestible energy. Commercial silver perch farmers were consulted about feeding practices for large fish (>500 g) and at water temperatures below 12°C, and winter feeding practices for other warmwater species were used to complete the strategy. In the feeding experiments, growth and FCR increased with increasing feeding rates to a level above which only FCR increased. Optimal feeding rates and frequencies were those which resulted in maximal growth, while minimizing effort (feeding frequency) and FCR. The highest feeding frequency required for maximal growth, including that of small fingerlings was twice (2 ×) daily, and the optimal feeding rates varied with water temperature and size of fish. The optimal daily regimes were: small fingerlings (initial mean weight, 2.0 g) 7.5% 2 × at a mean temperature of 23.3°C; fingerlings (14.9–27.7 g) 7.5% 2 × at 27.1°C, 5.0% 2 × at 23.7°C and 2.0% 1 × at 16.8°C; and large silver perch (162.5–510.6 g) 0.5% 1 × daily or 1.0% on alternate days at 15.6°C, 1.0% 1 × at 17.3°C, 3.0% 2 × at 24.1°C and 2.0% 2 × at 27.9°C. It is suggested that regimes of 0.5% 1 × daily for fingerlings (<50 g) and 0.5% 1 × on alternate days for larger fish are used at temperatures of 9–12°C, and 0.5% 3 days week?1 and 0.5% 1 day week?1 for fingerlings and larger fish, respectively, at 6–9°C. Feed inputs should not exceed 150 kg ha?1 day?1 in ponds less than 0.3 ha and 100 kg ha?1 day?1 in larger ponds. Our research has established a feeding strategy for silver perch based on restricted rations.  相似文献   

16.
Common snook (Centropomus undecimalis) is one of the most important marine species under commercial exploitation in the Gulf of Mexico; for this reason, interest in developing its culture is a priority. However, larviculture remains as the main bottleneck for massive production. In this sense, our objective was to determine the changes of digestive enzymes activities using biochemical and electrophoretic techniques during 36?days of Common snook larviculture fed with live preys (microalgae, rotifers, and Artemia). During larviculture, all digestive enzymatic activities were detected with low values since yolk absorption, 2?days after hatching (dah) onwards. However, the maximum values for alkaline protease (6,500?U?mg?protein?1), trypsin (0.053?mU?×?10?3?mg?protein?1), and Leucine aminopeptidase (1.4?×?10?3?mU?mg?protein?1) were detected at 12 dah; for chymotrypsin at 25 dah (3.8?×?10?3?mU?mg?protein?1), for carboxypeptidase A (280?mU?mg?protein?1) and lipase at 36 dah (480?U?mg?protein?1), for ??-amylase at 7 dah (1.5?U?mg?protein?1), for acid phosphatases at 34 dah (5.5?U?mg?protein?1), and finally for alkaline phosphatase at 25 dah (70?U?mg?protein?1). The alkaline protease zymogram showed two active bands, the first (26.3?kDa) at 25 dah onwards, and the second (51.6?kDa) at 36 dah. The acid protease zymogram showed two bands (RF?=?0.32 and 0.51, respectively) at 34 dah. The digestive enzymatic ontogeny of C. undecimalis is very similar to other strictly marine carnivorous fish, and we suggest that weaning process should be started at 34 dah.  相似文献   

17.
The great diversity of optimal UV irradiation doses are used for DNA inactivation in fish sperm forcing authors to repeat optimization of irradiation treatment every time. Analysis of sperm UV irradiation protocol for induction of gynogenesis showed the importance of sperm UV light absorption estimations. The UV absorption investigation in Siberian sturgeon sperm showed average extinction coefficient 7.69 × 10?8 ± 1.04 × 10?8 cm2. It is resulted in high heterogeneity of UV irradiation of undiluted sperm samples. Therefore, it is strongly suggested to specify doses only with defined concentration of spermatozoa; otherwise, the difference in absorbance level between samples can bring a significant error to optimal UV dose estimation. This was confirmed by UV-irradiated sperm motility investigation. Results of motility investigation of UV-irradiated sperm revealed high sensitivity of Siberian sturgeon spermatozoa motion mechanisms to UV irradiation, with complete loss of motility after homogeneous UV irradiation at doses above 2,000 J/m2. Partial gynogenesis was conducted using diluted and undiluted sperm. Ploidy level of hatched larvae was estimated by flow cytometry. Percentage of haploid hatched larvae revealed sperm DNA inactivation efficiency. The highest percentage of haploid putative gynogenotes 19.67 ± 4.19 % was obtained at UV irradiation dose 200 J/m2 with sperm diluted to 1:4.  相似文献   

18.
Abstract

Two trials evaluated winter feeding strategies for small golden shiners, Notemigonus crysoleucas destined for the crappie, Po-moxis spp. market. Fish were stocked at 615 g/pool (approximately 127 or 158 fish/m2, trials 1 and 2, respectively) in aerated, 5.9-m2 pools for 100 and 104 days, respectively. In the first trial, fish were fed a commercially available 32% crude protein crumbled, extruded pellet once daily at feeding rates of 0% (fertilizer only), 1%, or 2% of body weight/day (bw/d) on days when the afternoon air temperature was >7°C. Unfed fish lost weight, and their condition declined. Fish fed at 1% maintained both weight and condition, while fish fed at 2% gained weight. Fish in the second trial were fed once daily at 1% bw/d on those days when the air temperature was either >7°C or >10°C, with either a commercially available crumbled, extruded pellet or a sinking pellet (compressed). Fish fed crumbles were in significantly better condition at harvest than fish fed a sinking pellet, regardless of trigger feeding temperature, although average weight was not significantly different. Feeding on days with air temperatures >7°C provided 13% more feeding days than a trigger temperature >10 °C, and fish grew more over the winter. Two condition indices, relative weight (Wr) and Fulton's condition factor (K), proved useful in evaluating the condition of golden shiners cultured using different feeding strategies.  相似文献   

19.
Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow‐through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL?1: ≈1, 8, 25, 42 and 58 cells μL?1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros neogracile and Tisochrysis lutea (1:1 cell volume) maintained throughout the whole larval life at a concentration of 1500 μm3 μL?1 allowed the best larval development of O. edulis at 25°C with high survival (98%), good growth (16 μm day?1) and high settlement success (68%). In addition, optimum larval development (survival ≥97%; growth ≥17 μm day?1) and settlement (≥78%) were achieved at 25 and 30°C, at microalgae concentrations of 1500 μm3 μL?1. In contrast, temperature of 20°C led to lower development (≤10 μm day?1) and weaker settlement (≤27%), whereas at 15°C, no settlement occurred. The design experiments allowed the estimation of the maximum surface‐area‐specific ingestion rate  = 120 ± 4 μm3 day?1 μm?2, the half saturation coefficient {XK} = 537 ± 142 μm3 μL?1 and the Arrhenius temperature TA = 8355 K. This contribution put a tangible basis for a future O. edulis Dynamic Energy Budget (DEB) larval growth model.  相似文献   

20.
We isolated the rotifers Brachionus ibericus and Proales similis from the sediment of shrimp tanks and studied their individual demographic characters and competition between them at two food levels (0.25?×?106, 1.00?×?106 cells ml?1 of Nannochloropsis oculata at 25 °C) and salinities ranging from 10 to 30‰. Our hypothesis was that growth rates would be higher with increasing food levels and salinities. Observations were taken twice a day for life table studies and daily once for population growth experiments. Using survivorship and fecundity data, we derived various life history variables. Although the average life span (7.6?±?0.4 days) and gross reproductive rate (33.8?±?2.9 neonate female?1 day?1) of B. ibericus were higher than those of P. similis (average life span 5.4?±?0.6 days and gross reproductive rate 13.0?±?0.6 neonate female?1 day?1), the population growth experiments showed that P. similis had higher r values (0.32?±?0.005 day?1) than B. ibericus (0.23?±?0.002 day?1) at 1.0?×?106 cells ml?1 of N. oculata. The rotifer P. similis was more adversely affected due to the presence of B. ibericus than vice versa. The data are important for developing techniques for a large-scale culture of these rotifers as food in aquaculture.  相似文献   

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