首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 640 毫秒
1.
Large pond-reared Penaeus monodon were used in three maturation and spawning trials lasting 60 days. One group of prawns was fed a fresh diet only; a second group was supplied a fresh diet combined with a formulated pelleted diet; a third group of prawns received the formulated diet only. Total maturation (N = 66 and 55) and spawning events (N = 63 and 48) were much greater for prawns receiving only the fresh diet, and the combined fresh with formulated diets, respectively. Prawns receiving only the formulated diet matured only 12 times, and spawned 11 times. Prawns fed a fresh diet produced significantly more eggs than those given a formulated diet alone. No significant differences were seen in eggs produced per spawning event, % fertilization, % hatch or % metamorphosis from egg to protozoea. Female prawns exhibited greater mortality than did males, while females on the fresh diet only had greater mortality than did other females, presumably due to greater handling and other stress factors. Female prawns on a combined diet had lower mortality than other females. The formulated diet used is suitable for supplementation, but not as a sole maturation food.  相似文献   

2.
Sperm quality, as determined by visual examination and by reaction with “egg-water” was not significantly different (P > 0.05) for sperm obtained by electro-ejaculation from ablated or non-ablated pond-reared Penaeus monodon. Nor was sperm quality different between pond-reared and wild-caught prawns. Normal sperm, determined by appearance, ranged from 17.1 to 21.0%, while reactive sperm ranged from 1.5 to 3.0%. There were, however, significant correlations (P < 0.01) between spermatophore weight and prawn weight (r= 0.73, N= 434). Male prawns weighing 4150 g produced on average spermatophores weighing 22.7 mg and containing 0.8 million sperm, while prawns weighing 61-90 g produced on average spermatophores weighing 56.6 mg with 2.5 million sperm. Ablation did not increase spermatophore size or sperm quality, although it significantly increased mortality of ablated males. Male prawns could be re-ejaculated at about weekly intervals with no change in sperm quality. Wild-caught female prawns artificially inseminated with spermatophores from electro-ejaculated males produced normal spawns with 51% average egg fertilization, and 41% nauplii hatch success. Nauplii hatch success following spawning increased from >60% for newly inseminated females to near zero after 30 days post-insemination, indicating spermatophore depletion and/or deteriorated sperm quality during spermatophore storage in the thelycum. The findings of the present study indicate that electro-ejaculation and artificial insemination are relatively simple and practical methods for improving captive reproduction performance of closed-thelycum prawns such as P. monodon, and that pond-reared and wild-caught males produced sperm of similar quality.  相似文献   

3.
Serotonin (5-hydroxytryptamine, 5HT) has been reported to induce ovarian maturation and spawning in the crayfish Procambarus clarkii and white Pacific shrimp Litopenaeus vannamei. The aim of this study was to explore the role of exogenous 5HT on the reproductive performance of the black tiger shrimp Penaeus monodon. 5HT solution was injected into domesticated P. monodon broodstock at 50 μg/g body weight and ovarian maturation and spawning were recorded. The presence of 5HT in the ovary and oviduct of P. monodon was also studied by immunohistochemistry and its levels in the ovary by enzyme link immunoabsorbance assay (ELISA). The 5HT-injected P. monodon developed ovarian maturation and spawning rate at the level comparable to that of unilateral eyestalk-ablated shrimp. Hatching rate and the amount of nauplii produced per spawner were also significantly higher in the 5HT-injected shrimp, compared to the eyestalk-ablated shrimp. 5HT-positive reactions were found in the follicular cells of pre-vitellogenic oocytes, in the cytoplasm of early vitellogenic oocytes and on the cell membrane and cytoplasm of late vitellogenic oocytes. 5HT in the ovary was present at 3.53 ± 0.26 ng/mg protein level in previtellogenic stage and increased to 17.03 ± 0.57 ng/mg protein level in the mature stage of the ovary. The results suggest a significant role of 5HT, possibly directly on the ovary and oviduct, on the reproductive function of female P. monodon.  相似文献   

4.
The combined effects of temperature (23 and 27 °C), light intensity (1100 and 2 lux or ≈ 21.5 and 0.04 μEinst m?2 s respectively) and photoperiod (10 h light/14 h dark and 14 h light/10 h dark) on ovarian maturation and spawning performance of ablated pond‐reared Penaeus merguiensis were investigated in a 51‐day experiment. The results showed that temperature was the most influential factor, followed by light intensity, whereas the effect of photoperiod was minor. As the maturation process progressed, the effects of temperature and light intensity became stronger while that of photoperiod became less significant. Temperature significantly affected all the reproductive parameters assessed. Prawns in the 27 °C treatments outperformed those in the 23 °C treatments (P < 0.05). The effect of light intensity was found to have a significant effect (P < 0.05) only on the proportion of spawners (prawns that spawned) and spawning rate. More prawns spawned at a higher rate under dim light (2 lux) than under strong light (1100 lux). Photoperiod affected only the proportion of prawns reaching stage III of ovarian development (P < 0.05). There were interactions between temperature and light intensity affecting the proportion of prawns reaching stage III and, although not significantly, the proportion of spawners (P = 0.177), fecundity (P = 0.134) and survival (P = 0.061). Overall, it is recommended that a combination of 27 °C, 2 lux and 10 h light/14 h dark is suitable for the maturation of pond‐reared P. merguiensis. There were indications that temperature can be used to control the rate of ovarian maturation. Also, it is possible to increase light intensity up to 1100 lux in P. merguiensis hatcheries if prawns are ablated.  相似文献   

5.
This study examined the dietary requirement of arachidonic acid (ARA) when that of linoleic acid (LOA), the natural precursor to ARA, was also satisfied with linolenic acid (LNA) and also with and without the other key dietary highly unsaturated fatty acids (HUFA). Growth by prawns fed diets supplemented with ARA was poorer than in diets where it was not present. Supplementation of ARA to diets with either optimized HUFA or just optimised poly unsaurated fatty acids (PUFA) (i.e. LOA, LNA) resulted in poorer growth. Growth was poorest by prawns (215 ± 13%) fed diets with ARA supplemented at 20% of the total fatty acids but including 7% LOA, 21% LNA and 4% of both eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Growth was best in prawns fed diets devoid of ARA but with 7% LOA and 21% LNA (350 ± 19%). Prawns fed the reference diet (348 ± 21%) and the other diet devoid of ARA but containing about 7% LOA, 21% LNA and 4% of both EPA and DHA (345 ± 18%) had similar growth. The growth responses were not effects of altered lipid or fatty acid digestibilities. Indeed supplementation of ARA to the diet marginally improved the digestibility of the total neutral lipid in the diet and the digestibilities of some other dietary fatty acids. The amount of lipid in the digestive glands of prawns fed with the diets was reduced by the inclusion of ARA in the dietary lipids. Composition of the lipids in the digestive gland (DG) of the prawns was almost directly related to the composition of their dietary lipids. The proportion of ARA in the total fatty acids increased with level of supplementation of dietary ARA. An increased level of dietary ARA reduced the proportion of EPA, DHA in the DG lipid and also the total n‐3 and n‐6 fatty acids in the DG lipid. The results of this study support that addition of ARA to the diet of Penaues monodon when the other key essential fatty acids (EFA) have been optimized, does not improve their growth performance. It is suggested that key cause for this response may lie in the importance of the balance of the n‐3 to n‐6 fatty acids in the diet of these animals.  相似文献   

6.
Industry acceptance of alternative sulphite treatments for preventing blackspot in prawns is limited. This study demonstrated that dipping prawns (Penaeus monodon and Fenneropenaeus merguiensis) in a less concentrated solution for longer times provides better control of blackspot formation than the recommended 2 minute dip in 50 mg/L 4-hexylresorcinol. Protection improved as modified dipping times and solution concentrations increased. Blackspot protection provided by most of the modified dip methods was more effective than a 1 minute dip in 1% sodium metabisulphite. The recommended 2 minute dip method should be limited to 125 kg when used on consecutive 25-kg batches of prawns. Yields increased by less than 10% even after 72-hour exposure in the modified dip. The new dipping method meets most international residue standards when applying the modified treatments evaluated.  相似文献   

7.
A quantitative, low‐cost test based on haemagglutination (HA) using chicken erythrocytes was developed to indicate the viral load of Australian yellowhead‐like virus, gill‐associated virus (GAV), in Penaeus monodon. The study tested the haemolymph, gill, lymphoid organ, heart, sub‐cutaneous tissue, eye stalk, pleopods, uropods and the central nerve cord for agglutination activity in 100 prawns, with the haemolymph and gill tissue giving the highest end‐point titres of 1:1370 and 1:361 respectively. The sensitivity of the test was demonstrated by testing two different populations of P. monodon, which showed a highly significant difference (P<0.001) in HA activity, indicating a difference in viral load. By testing three other penaeid prawn species (n=20), Penaeus esculentus, Penaeus merguiensis and Penaeus longistylis, and the crayfish Cherax quadricarinatus, it was demonstrated that natural agglutinins were not causing the high agglutination in the population of P. monodon being tested. It was also demonstrated that there was no effect of freezing and thawing of samples on HA activity. The speed and low cost of this test makes it a very useful tool, particularly in the developing world, for on‐farm testing of penaeid prawns to indicate yellowhead virus and GAV loads which can contribute to management practices with respect to harvesting of ponds.  相似文献   

8.
The aim of this experiment was to determine the effects of dietary inclusion with mannan oligosaccharide (Bio‐Mos, Alltech, Nicholasville, KY, USA) on growth, survival, physiological and immunological conditions and gut morphology of the black tiger prawn (Penaeus monodon). Five diets supplemented with MOS at 0 g kg?1 (control diet), 1, 2, 4 and 8 g kg?1 were fed to the prawn juveniles (0.4 ± 0.06 g, total weight) for the duration of 63 days. Growth was the highest (P < 0.05) when the prawns were fed the 1 g kg?1 MOS included diet. Wet tail muscle index (Tw/B), dry tail muscle index (Td/B) and tail muscle protein (Tmp) were higher (P < 0.05) in the prawns fed MOS included diets MOS compared with the prawns fed the control diet. Total haemocyte counts (THCs) of the prawns fed MOS included diets were higher (P < 0.05) than THCs of the prawns fed the control diet. Epithelium layer and epidermal cell density of the gut of the prawns fed 1 g kg?1 and 2 g kg?1 MOS diets were better than the prawns fed the control and other MOS diets. The results imply a positive effect of dietary supplementation of 1–2 g kg?1 MOS in the culture of black tiger prawns.  相似文献   

9.
A possible factor behind the successful use of polychaetes for prawn broodstock diet was investigated. Some steroid hormones in polychaetes have been suspected as being a potential component for prawn reproductive system development. Progesterone (P4) and 17-alpha hydroxyprogesterone (17α-OHP4) were extracted from polychaetes (Perinereis sp.) and female Penaeus monodon broodstock at various maturation stages. Concentration of both hormones was determined using RIA or HPLC. Immature natural polychaetes (2 months old) displayed the highest level of P4 and 17α-OHP4 compared to more mature ones (4, 6 and 8 months old polychaetes) (47.48 ± 6.70 ng P4/mg Protein and 143.74 ± 28.70 ng 17α-OHP4/mg Protein) whereas opposite results were received with cultured polychaetes in which a reverse relationship between P4 and its derivative with polychaete maturation was obtained. Diverse concentrations of both hormones may be linked with other factors such as types of polychaetes, habitats, seasons, and feed intakes. Twenty-four hours in vitro incubation of prawn previtellogenic oocytes with P4 or 17α-OHP4 extracted from polychaetes or with synthetic hormones, significantly increased percentages of vitellogenic oocytes and oocytes with cortical rod compared to the control with no hormones. P4 was more effective in enhancing the final maturation of oocytes while 17α-OHP4 had more effects on vitellogenic oocytes. Synthetic steroid hormones at equal hormone concentrations produced similar results to steroid hormones extracted from natural polychaetes.  相似文献   

10.
Oocyte maturation and gonadosomatic index (GSI) of eyestalk ablated Penaeus monodon females collected from the wild and from two first‐generation domesticated lines were assessed. Frequency and diameter of the different oocytes, and the intensity of oocyte atresia, were compared among groups through histological assessments of the sections of the middle ovarian lobe. Digitized images from ovary sections were used to record the frequency and diameter of different oocyte types. Spawning performance of the three groups were expressed in terms of the percentage of females that spawned at least once (productive females), time from eyestalk ablation to first spawning (latency period) and the number of spawnings per female stocked. Final ovarian maturation was attained in all groups, as indicated by the presence of mature oocytes with cortical rods (cortical oocytes), dark‐green ovarian colour and high GSI values (5.83–6.86%). However, domesticated groups presented significant larger immature oocyte types (previtellogenic and yolky oocytes) and smaller cortical oocytes compared with wild females, indicating a reduced vitellogenic activity during final oocyte maturation. Additionally, the frequency of atresia was comparatively higher for both domesticated groups, which could be related to their inferior spawning performance. The implications of these results on the reproductive potential and development of domesticated P. monodon are discussed.  相似文献   

11.
The reproductive performance of domesticated Penaeus monodon was assessed when fed on two experimental semi‐moist maturation diets varying in their arachidonic acid content for 21 days before ablation and throughout a 17‐day reproductive assessment. The biochemical composition of the two semi‐moist two diets was similar with the exception of arachidonic acid (ARA) content; the basal diet (BAS) consisting of 0.9 g kg?1 DM ARA (1.1% of total fatty acids) and the supplemented diet (ARA‐SUP) consisting of 5.0 g kg?1 DM ARA (5.8% of total fatty acids). ARA/EPA and ARA/DPA ratios were 0.1 in the BAS diet and 0.5 in the ARA‐SUP diet. Fatty acid composition of the spawned eggs was comparable between diets with the exception of ARA concentration, which was higher in the ARA‐SUP (8.95 ± 0.44 g kg?1 DM) than the BAS (3.23 ± 0.17 g kg?1 DM) (P < 0.0001). The cumulative percentage of females spawning (mean ± SE after 17 days) (31.9 ± 7.0%; 24.1 ± 1.3%), number of spawnings per female (0.48 ± 0.1; 0.29 ± 0.02), and eggs per female (62 520 ± 16 935; 44 521 ± 9914) was significantly (P < 0.0001) higher for the ARA‐SUP than the BAS. Results of this study suggest that arachidonic acid plays a key role in promoting egg development and spawning in P. monodon.  相似文献   

12.
In this study, the phenotypic and pathogenic properties in freshwater host were characterized in 14 strains of halophilic Vibrio harveyi isolated from infected marine black tiger shrimp, Penaeus monodon. The lysogenic phenotype was assayed via prophage excision and mitomycin C induction. The bacteria were grouped into two types, corresponding to lysogenic and non‐lysogenic strains. The pathogenicity was determined via direct injection of bacterial cultures into post‐larval juvenile giant freshwater prawns, Macrobrachium rosenbergii De Man. All of the infected prawns showed similar symptoms and inflamed hepatopancreas. The V. harveyi isolates derived from the first‐injected infected prawns were re‐isolated and re‐injected into healthy giant freshwater prawns, in which they retained similar infectivity. Both lysogenic and non‐lysogenic Vibrio spp. showed identical virulence associated with 100% mortality within one day post‐injection. TEM micrographs showed hepatopancreatic nuclear deformation and lipid breakdown caused by lysogenic γ‐hemolytic VL19 and non‐lysogenic β‐hemolytic V33. However, the V33 strain was associated with severely disrupted mitochondria. None of the V. harveyi strains was able to produce a biofilm. Together, our findings indicate that the lysogenic and non‐lysogenic halophilic V. harveyi isolated from marine shrimps may use different virulence factors that are responsible for their pathogenicity in freshwater prawns.  相似文献   

13.
This study used a small amount of several lyophilized microalgae (5% of dry weight in aquafeed) to feed adult black tiger prawns Penaeus monodon for 4 weeks, aiming to promote prawn health and nutritional level. Apart from slight increases in ash content, chlorophyll and β‐carotene in the microalgae‐added feeds, there was no difference from the commercial diet in their biochemical composition. After 28 days of feeding, prawn body length, body weight, condition index and also total amount of fatty acids and amino acids of the flesh (2nd abdominal segment) were not affected by microalgae additives as compared with the control commercial diet‐fed group (P > 0.05). Prawns fed the microalgae diets, however, had a relatively better survival rate than those fed the commercial control diet, although no statistical significance was detected in any except for the diatom Melosira sp. The elevated survival rate was concomitant with a higher phagocytosis rate and superoxide dismutase (SOD) activity in the immune response analysis, and significantly a higher astaxanthin content detected in the tail muscle of microalgae diet‐fed prawns (5.36–10.78 ng of astaxanthin per gram of muscle DW). These findings demonstrated that low‐inclusion microalgal additives in prawn diets over a short feeding period (4 weeks) can result in healthier adult prawn, and hence could contribute to the development of an optimized feeding strategy for prawn aquaculture close to harvesting.  相似文献   

14.
A 2-month feeding trial was conducted toevaluate the reproductive performance andoffspring quality of mud crab (Scyllaparamamosain) females fed either a mixture offresh food items (squid, shrimp, trash fish andArtemia biomass) or two experimentaldiets developed for penaeids. Before testinitiation, mud crab females with an averageindividual wet weight of 200–300 g wereacclimated for 2–3 days and reared together inone concrete tank of 2.0 × 0.5 × 8 m untilspawning. After spawning, the spent spawnerswere unilaterally eyestalk ablated and randomlydivided (20 animals/treatment) over three tanksof the same size and subjected to the dietarytreatments. Spent spawners were used toeliminate the effect of feeding history.There were only minor differences inreproductive performance between dietarytreatments. No differences were observed in theduration of the latency period from eyestalkablation to spawning. Fecundity was onlymarginally higher for the broodstock fed thecontrol diet. Also egg quality seemed onlyslightly affected by the treatments. Egghatching rates were slightly higher in crabsfed the formulated diets compared to thosecrabs fed the fresh diet. The onlystatistically significant difference (p < 0.05) observed however was in egg hatchingrate between the control diet and diet A2. Incontrast, the crabs fed the fresh diet producedstronger larvae as determined by a starvationtest.We therefore conclude that artificial dietsresulted in reproduction success comparable tothe use of fresh food. The nutritionalcomposition of the artificial diets couldhowever be improved in order to produce larvaeof optimal quality. Based on our researchfindings, the protein level and n-3 HUFA levelin the diet warrants further investigation inthis respect.  相似文献   

15.
为了研究法尼酸甲基转移酶(Farnesoic acid O-methyltransferase,FAMeT)在脊尾白虾卵巢发育中的作用,采用实时荧光定量PCR方法分析连续3次繁殖过程中卵巢不同发育时期(Ⅰ-Ⅴ期)大颚器、卵巢和肝胰腺中FAMeT基因的表达规律.结果显示,FAMeT基因在鳃、心脏、肌肉、肝胰腺、卵巢、大颚器等组织中均有稳定的表达,其中,在鳃和大颚器的表达量显著高于其他组织;在卵巢发育周期内,FAMeT基因在大颚器、卵巢、肝胰腺中的表达量变化趋势基本一致,从Ⅰ-Ⅳ期逐渐下降,到Ⅴ期时表达量上调;在每个卵巢发育时期,FAMeT基因在大颚器中表达量最高,约为卵巢中表达量的20-30倍.研究结果表明,大颚器是合成FAMeT的主要组织,脊尾白虾繁殖过程中FAMeT基因主要在卵巢发育前期表达,可以合成大量甲基法尼酯(Methylfarnesoate,MF),促进卵巢发育.  相似文献   

16.
  相似文献   

17.
The effect of claw ablation on the growth and survival of juvenile Macrobrachium rosenbergii was tested; two sets of controls were used consisting of a group of intact prawns, and an additional group whose first pair of walking legs had been ablated so as to serve as controls for injury and regeneration. Survival among claw-ablated prawns was twice as high as that for the two control groups. Intact prawns weighed more than those with ablated appendages. During the experiment the coefficient of variation of mean weight decreased in the claw-ablated group. It increased in the intact prawns and was relatively stable in the group with ablated first pair of walking legs. The biomass of the claw-ablated group doubled within the test period while that of the controls was stable because the gain in mean weight and the mortalities counterbalanced each other.  相似文献   

18.
The contents of three essential fatty acids, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from wild Penaeus monodon broodstock were evaluated in comparison with natural diet fed P. monodon. Spermatophores of wild male broodstock contained higher levels of AA than those of artificial diet fed males. Polychaetes had higher proportion of AA to EPA and DHA at 5.8:5.5:1 in mud polychaetes followed by 12:7:1 in sand polychaetes, while DHA was a preferential n‐3 highly unsaturated fatty acid (HUFA) in squids and fish. The experimental feed was constructed to simulate the HUFA profile of polychaetes (AA:EPA:DHA as 5:1:1) and then fed to farmed male black tiger prawn broodstock for 1 month. The results exhibited comparable reproductive characteristics to wild male suggesting the possibility of replacing wild males with pond‐reared males. Rearing farmed males in a test unit for a month did not reduce the quality of prawn sperm. Reproductive performance indices (sperm sac weight, total number of sperm, percentage of live sperm, percentage of abnormal sperm) from the males of all treatments were not statistically different except in males fed with pellets. Control (live feeds) and combined diet provided better reproductive performance in pond‐reared males. Analysis of AA, EPA and DHA in reproductive tissues, hepatopancreas and muscle of treated animals in each treatment revealed an accumulation of dietary HUFA into reproductive tissues. No evidence of transfer of HUFA from hepatopancreas or muscle to spermatophore was found.  相似文献   

19.
The effect of methyl farnesoate (MF) administration on the vitellogenesis of the penaeoidean shrimp, Sicyonia ingentis, was studied. The short‐ and long‐term treatment effects as well as the effect of two MF injection regimens (0.1 and 1.0 μg MF/injection) were evaluated. The studies were also carried out to understand the pattern of vitellogenesis in eyestalk ablated adult and juvenile shrimps. A combination of endpoints, haemolymph vitellogenin (Vg) levels, gonadosomatic index (GSI) and histology, was used to study the effect of these treatments. The GSI increased in all the MF‐treated shrimp compared with the control shrimps. Although haemolymph Vg levels declined over the experimental period in all the treatments, the Vg levels decreased significantly only in the short‐term treatment with 1.0 μg MF. Similarly, haemolymph protein level also declined over the experimental period in all the treatment groups. However, except in the long‐term treatment with 0.1 μg MF, all treatments showed a significant decrease in haemolymph protein level. Conversely, in all eyestalk ablated adults and juveniles, haemolymph Vg, total protein and GSI increased over the experimental period, all of which were higher than the concurrent control. The discrepancy in the vitellogenic pattern between MF‐treated and eyestalk ablated shrimp was possibly due to the difference in the ovarian phase of the initial control. Although unilateral eyestalk ablation failed to induce vitellogenesis in juveniles, bilateral ablation induced vitellogenesis, which indicates that juveniles are competent to undergo vitellogenesis.  相似文献   

20.
The transformer‐2 gene (tra‐2) plays a key role in the sexual differentiation regulatory hierarchy. In this study, tra‐2 gene homologs designated as Mntra‐2 was cloned and characterized from Macrobrachium nipponense. The full‐length cDNA of Mntra‐2 consists of 1724 bp with an open reading frame (ORF) encoding 192 amino acids, an 827 bp 5′‐untranslated region (UTR) and a 318 bp 3′‐UTR. The predicated molecular mass of Mntra‐2 was 20.805 kDa with an estimated theoretical isoelectric point of 10.36. The deduced amino acid sequence shares high homology with Penaeus monodon. Real‐time quantitative polymerase chain reaction (RT‐qPCR) analyses demonstrated that the expression levels of Mntra‐2 varied significantly during different developmental stages of embryogenesis, larvae, and post‐larvae and in various adult tissues. During embryogenesis, the expression level of Mntra‐2 was slightly higher at the cleavage stage than at the blastula stage, and reached the highest level at the nauplius stage. During the larvae, the Mntra‐2 expression gradually increased from 1 d larvae post hatch (L1) to L10 and decreased to a lowest level at the end of metamorphosis. During the post‐larvae, the Mntra‐2 expression was higher level at the 5 d after the metamorphosis (P5). RT‐qPCR showed the Mntra‐2 mRNA was expressed in ovary, testis, muscle, heart, abdominal ganglion, brain, and intestine with the highest level of expression in muscle and intestine. The results indicate that Mntra‐2 is an arthropods tra‐2 homolog and probably plays important roles in embryonic development and sex differentiation of M. nipponense .  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号