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1.
以湿地松茎段为外植体,诱导其丛生芽的形成,进而建立其再生体系。实验结果表明,所设计的培养基中改良GD+NAA0.5 mg/L+6-BA4 mg/L是湿地松茎段丛生芽诱导的适宜培养基,外植体成活率相对较高,可以达到66.7%,其诱导率可达100%;丛生芽生长和发育的培养基为改良GD+0.5 g/LAC;由湿地松茎段诱导形成的芽苗生根率较低,在1/2WPM+NAA0.15 mg/L+IBA1.5 mg/L中,其生根率为最高,仅有27.8%;在移栽基质蛭石∶珍珠岩=3∶1中,生根的小植株可正常生长。 相似文献
2.
通过正交试验等方法开展的金焰彩栾离体培养技术研究结果表明:从金焰彩栾播种后生长3个月的苗上选取外植体,经过0.1%Hg Cl2消毒5 min,灭菌成活率为86.8%;改良MS培养基附加BA,NAA,IBA、IAA等植物生长调节剂可有效促进试管芽苗增殖和生根,其中BA 0.02 mg/L+IBA 0.02 mg/L+NAA 0.03 mg/L+IAA 0.08 mg/L组合培养基为最适宜的继代培养基,增殖倍数达3.8,生长高度为4.8 cm;带根试管苗移栽于泥炭(V)∶黄心土(V)=6∶4的混合基质,控制温度23~30℃,相对湿度85%,保湿10~15 d,其间适当遮荫,20 d后成活率达92.8%。 相似文献
3.
花叶络石离体快繁技术研究 总被引：1，自引：0，他引：1
花叶络石是攀援、垂直等立体绿化好材料,具有较高观赏价值。通过正交试验等方法对花叶络石茎段组织培养外植体灭菌、玻璃苗预防、增殖培养、根诱导等关键技术进行研究。结果表明:在镇江句容花叶络石的最佳取材时间为4月中下旬,在引进日本种源中筛选优良类型的母株上选取外植体,经过用0.1%HgC l2消毒灭菌10 s,成活率为80.5%;添加3.5%的白砂糖和增加培养容器的透气性,能很好地预防玻璃苗的发生;MS附加TDZ、BA、NAA等植物生长调节剂可有效促进试管芽苗增殖和生长,其中TDZ 0.01 mg/L BA 2.0 mg/L NAA 0.01 mg/L组合最适宜增殖与生长,增殖倍数达5.6,生长高度为2.8 cm;适宜的生根培养基为1/2MS IBA 0.5 mg/L NAA 0.3mg/L CH 100 mg/L S 2%,生根率可达95%;经生根的试管苗移栽于蛭石(V)∶珍珠岩(V)∶泥炭(V)=6∶3∶1的混合基质中,控制温度20~30℃,相对湿度85%~90%,保湿15~20 d,其间适当遮荫,22 d后成活率91.5%以上。 相似文献
4.
以照山白杜鹃(Rhododendron micranthum)试管苗的茎段为外植体,研究不同激素组合对其外植体诱导、丛生芽增殖及试管苗生根的影响.结果表明:最适丛生芽诱导培养基为Read+ ZT 4.0 mg/L+ NAA 0.05 mg/L+蔗糖(3％),诱导率达92.41％;最适丛生芽增殖培养基为Read+ ZT 3.0 mg/L+ NAA 0.1 mg/L+蔗糖(3％),增殖系数达7.56;生根培养基为Read+ IAA 0.5mg/L+蔗糖(2％),生根率达92.5％;NAA不适宜照山白杜鹃生根诱导;将试管苗移栽到松毛土∶泥炭土∶河沙=1∶2∶1的基质中,成活率达89％. 相似文献
5.
以黄樟秋稍带腋芽茎段为外植体,建立并优化了黄樟组培繁育体系,研究基本培养基、不同激素及配比对黄樟茎段不定芽诱导、组培苗增殖及生根的影响。结果表明:黄樟带腋芽茎段最佳启动培养基配方为改良MS+4.0 mg/L 6-BA+0.5 mg/L NAA,在此培养基中不定芽培养7 d开始萌动,诱导率可达76.67%;不定芽继代培养基的最适宜配方为改良MS+2.0 mg/L 6-BA+0.5 mg/L NAA,在此培养基中不定芽生长健壮,增殖系数为3.78;幼苗生根培养基的最适宜配方为改良1/2 MS+0.5 mg/L IBA+0.5 mg/L IAA,在此培养基中幼苗培养13 d开始发根,生根率达85.56%。上述组织培养技术条件,可有效提高黄樟茎段的不定芽诱导率和生根率,为黄樟优良品系的工业化育苗奠定了基础。 相似文献
6.
通过对南京椴无菌实生苗茎段的组织培养,探索南京椴带芽茎段分化、增殖及生根的最佳培养条件.结果表明,最适宜诱导南京椴不定芽分化的培养基为MS+BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖30.0g/L+琼脂5.2 g/L,诱导分化率为100%;最佳芽增殖及继代培养基为MS+BA 0.5～1.0 mg/L+NAA 0.05 mg/L+蔗糖30.0 g/L+琼脂5.2 g/L,繁殖系数达4.0～5.0;在生根培养基(1/2MS+BA 0.1 mg/L+IBA 0.5 mg/L+蔗糖20.0 g/L+琼脂5.2 g/L)中添加5.0 g/L AC能明显促进不定根形成,40 d后生根率达56.6%;生根苗移栽至泥炭土中,成活率达80.0%. 相似文献
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8.
通过正交试验等方法对北美枫香"秋霞红"组织培养技术进行了研究。结果表明:在江苏扬州最佳取材时间为4月下旬,选取外植体,经过0.1%Hg Cl2消毒12 min灭菌,成活率为78.7%;添加VC35 mg/L能有效预防试管苗褐变的发生,增加培养容器的透气性可减少试管苗玻璃化发生;2/3MS附加TDZ、BA、NAA等植物生长调节剂可促进试管苗增殖和生长,其中TDZ 0.2 mg/L+BA 1.0 mg/L+IBA 0.1 mg/L+VC35 mg/L组合最适宜增殖与生长培养,增殖倍数达4.5,生长高度为3.8 cm;适宜的生根培养基为1/3 MS+IBA 0.5 mg/L+NAA 0.3 mg/L+AC 3 000mg/L+S 2%,生根率可达92%;经生根的试管苗移栽于泥炭、黄心土为7∶3(V∶V)的混合基质,控制温度23~30℃,相对湿度90%,保湿12~18 d,其间适当遮荫,30 d后成活率达90.8%。 相似文献
9.
杂交鹅掌楸的离体培养和植株再生研究 总被引：2，自引：0，他引：2
将杂交鹅掌楸3年生母树的饱满冬眠顶芽、侧芽接种在MS培养基上培养20d后，继以在改良MS(铵态氮与硝态氮的质量分数比为1：1)培养基上培养15d，2种培养基均含有IBA0．15mg／L。启动阶段在培养基中添加BA1．0mg／L ZT1．0mg／L TDZ0．05mg／L VC7．5mg／L S30g/L。继代增殖培养时添加BA1．0mg／L ZT0．5mg／L TDZ0．05mg／L VC7．5mg／L S30g／L，有效芽苗增殖倍数达3．8。壮苗培养时添加BA0．5mg／L ZT0．5mg／L TDZ0．05mg／L VC7．5mg／L S30g/L，芽苗粗壮，高达3．5cm。生根培养基1／2MS添加IBA0．1mg／L NAA0．1mg／L S20g／L 活性碳0．2％，生根率达75％。移栽至砻糠灰与黄心土按3：1(体积比)的混合基质中．成活率最高达65％。 相似文献
10.
"长蕊绿萼"梅花具节茎段组织培养初报 总被引：3，自引：3，他引：3
诱导“长蕊绿萼”梅花具节茎段启动生长与增殖的培养基，以改良WPM BA2．0mg／L ZT2．0mg／L IAA0．1mg/L GA30．5mg／L较为适宜；以改良WPM BA2．0mg／L ZTl．5mg/L IAA0．2mg／L继代培养，培养温度为28℃，光照度150Dlx，光照时间14h/d；生根处理用培养基为1／2WPM IBA0．5mg／L NAA0.3mg／L。 相似文献
11.
《Scandinavian Journal of Forest Research》2012,27(5):427-435
In Scandinavia, moose (Alces alces L.) sometimes cause severe browsing damage to economically-important pine. Moose-vehicle accidents have spurred construction of fences along roads, and these may interfere with moose migration between summer and winter ranges, or the road alone may be a barrier. If this happens and moose build up along roads, landowners may suffer economically. Therefore, this study investigated whether roads, fences or other factors influence the use of young pine stands by moose. Eighty stands along roads in northern Sweden were evaluated in which individually-browsed branches were counted on 9972 pines. Moose browsing was not significantly related to birch (Betula pendula Roth, B. pubescens Ehrh.) density, nor did it differ between pines (Pinus contorta Douglas or P. sylvestris L.). However, increased pine density, site productivity and proximity to a highway were associated with increased browsing. Further large-scale studies are needed to understand moose habitat selection and the effects of roads. 相似文献
12.
《Scandinavian Journal of Forest Research》2012,27(3):206-217
The root systems of 2-yr-old Picea glauca, Picea mariana and Pinus banksiana seedlings were submitted to various frost temperatures during an artificial frost to induce different levels of root damage. Frost-damaged and control seedlings were placed in a greenhouse under high and low soil moisture regimes. Seedling growth and physiology were evaluated periodically. Seedling survival was reduced when root damage reached levels of 60-80%. Root systems of all three species showed partial to total recovery by the end of the experiment. In general, root freezing damage caused reductions in seedling growth, with these reductions becoming less significant over time. Root damage had little to no effect on black spruce and jack pine seedling physiology, while white spruce CO 2 uptake decreased with increasing root damage. Shoot nitrogen content of all three species decreased slightly with increasing root damage. 相似文献
13.
《Scandinavian Journal of Forest Research》2012,27(1):53-61
This study investigated the stand structure in pine, spruce and deciduous forests in the border district of Finland and Russia. A total of 46 mature forest stands was selected as pairs, the members of each pair being as similar as possible with respect to their forest site type, age, moisture and topography. The stands were then compared between the two countries by means of basal areas and number of stems. The proportions of dominating tree species were 2-12% lower, and correspondingly the proportions of secondary tree species higher, in Russian forests. The density of the forest stock was also higher in each forest type in Russia. The forests in the two countries differed most radically in terms of the abundance of dead trees. The amount was two to four times higher in Russian deciduous and spruce forests, and in pine forests the difference was 10-fold. The stand structures indicated that Russian coniferous stands, in particular, were more heterogeneous than intensively managed pine and spruce stands in Finland. 相似文献
14.
《Scandinavian Journal of Forest Research》2012,27(1):15-27
The root collar diameter and the height:diameter ratio are of particular importance in container-grown seedlings where a high density in the containers may produce spindly seedlings. Temperature regimes and light quality are known to affect plant growth. The aim of this study was to identify responses in Picea abies (L.) Karst. seedlings grown with light providing different red:far-red ratios and under temperature regimes with alternating day (DT) and night temperature (NT) from negative (DT < NT) to positive (DT > NT) difference (DIF) between DT and NT. Experiments were conducted in controlled environment chambers and in a daylight phytotron. Only limited thermoperiodic responses appeared in P . abies seedlings with respect to seedling height and dry weight accumulation. The formation of terminal buds, however, was clearly delayed in seedlings grown at negative DIF. The results indicate a requirement for day extension light that is high in far-red, to prevent terminal bud formation under natural short-day conditions. An extended study should be conducted to clarify the minimum level of light intensity and the optimal light quality needed to prevent terminal bud formation under natural short-day conditions. 相似文献
15.
本文分析了CAD在设计中引起的正面和负面影响,并进行了系统的阐述,从而使设计者在应用中能够保持客观的态度。 相似文献
16.
《Scandinavian Journal of Forest Research》2012,27(2):131-138
The effects of soil scarification (mounding), slash removal and clear-cut age on the natural regeneration in clear-cuts was evaluated using data from four sites in southern Sweden. The treatments were carried out during a good seed and establishment year for birch ( Betula pubescens Ehrh. and B. pendula Roth). Scarification had the strongest positive effect on the density of naturally regenerated seedlings, especially in birch, but also in pine ( Pinus sylvestris L.) and spruce [ Picea abies (L.) Karst]. Slash removal had a positive effect on birch density. No statistically significant effect of clear-cut age was found. In addition, no statistically significant interactions between clear-cut age and scarification or slash removal were found. The ingrowth of field vegetation was the fastest in areas that were not scarified, less rapid in areas scarified on old clear-cuts, and the slowest after scarification in fresh clear-cuts. In conclusion, it may be possible to control the density of birch during a good establishment year for birch. If birch is desired, the best combination of treatments is to remove the slash and scarify; otherwise, these treatments should be avoided. 相似文献
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18.
《Scandinavian Journal of Forest Research》2012,27(5):472-479
Based on an enquiry, risk perception among non-industrial private forest owners is described in relation to climate change and forestry hazards. Of the respondents, 11% took action to remedy the effects of climate change. Out of a given set, hazards were ranked according to each respondent's experience of recent substantial financial loss to the estate and in relation to his or her willingness to make investments aimed at risk reduction. For each hazard, the respondent assessed the risk in four classes ranging from very high to negligible risk. Six hazards were considered most problematic in all three aspects: browsing damage, falling timber prices, damage by wind, spruce bark beetle, root rot and pine weevil. A majority of the respondents claimed to take action to reduce the risk associated with at least one hazard, while 35% did not know whether they did. Excluding climate change, the need for decision support was the largest in relation to damage by wind owing to a combination of perceived high risk and a high level of ignorance in relation to whether risk-reducing measures were taken. 相似文献
19.
Srisuda Thippayarugs Banyong Toomsan Patma Vityakon Viriya Limpinuntana Aran Patanothai Georg Cadisch 《Agroforestry Systems》2008,72(2):137-148
In situ produced plant residues contain a mixture of different plant components of varying quality. To assess synergistic
or antagonistic interactions occurring during the decomposition and mineralization of such mixtures, individual plant parts
(stems, leaves, leaf litter and roots) or the mixture of stems, leaves and leaf litter of the agroforestry species pigeonpea
(Cajanus cajan) or of crop residues of peanut (Arachis hypogaea) or of the weed hairy indigo (Indigofera hirsuta) were incubated in pots for 19 weeks. Periodically, remaining plant residues were sieved out (>2 mm), weighed and N content
as well as soil mineral N determined. Above- and below-ground residues of peanut decomposed fastest and showed the largest
N release in agreement with their high N concentration and low-acid detergent fibre (ADF) : N ratio. Hairy indigo was hypothesized
to be of lower quality than pigeonpea because of its high-polyphenol content. However, it decomposed faster than pigeonpea,
largely because of the higher N and lower lignin concentration of its components. Ranking of individual plant components for
N mineralization resulted in the following pattern, leaves > leaf litter > roots > stems. In mixtures of the different plant
components a similar species order in decomposition was obtained, e.g. peanut > hairy indigo > pigeonpea. The amount of N
released from the mixture was dominated by stem material that comprised 46–61% of the mixture. The interactions in mixtures
were relatively small for peanut (generally high-quality components) as well as for pigeonpea (low proportion of high-quality
components, i.e. N rich leaf material). However, a positive interaction occurred during later stages of N mineralization in
the mixture of hairy indigo as it had a significant proportion of N rich components and absence of highly reactive polyphenols.
Thus, for plants with low to intermediate chemical quality attributes, manipulating plant composition (e.g. by varying harvest
age, affecting stem and leaf proportions) will be important to obtain significant interactions during decomposition when its
components are mixed. 相似文献
20.
A. Rigueiro-Rodríguez M. R. Mosquera-Losada E. Gatica-Trabanini 《Agroforestry Systems》2000,48(3):245-256
The use of organic waste materials such as milk sewage as an organic fertilizer could have the dual advantages of organic-waste
disposal and reduced dependence on inorganic fertilizers. The effects of fertilization with (1) conventional mineral fertilization,
(2) milk sewage sludge at 40 kg N ha−1 target rate and (3) no fertilization on pasture production and tree growth were examined in an experiment consisting of two
pasture mixtures under a one-year-old Pinus radiata plantation with a density of 2500 trees ha−1. The two pasture mixtures were: (1) Dactylis glomerata L. var. saborto (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1); (2) Lolium perenne L. var. Tove (25 kg ha−1) + Trifolium repens L. group Ladino (4 kg ha−1) + Trifolium pratense L. var. Marino (1 kg ha−1). The experiment began in the spring of 1995 using a randomized block design with three replicates in Castro Riberas de Lea
(Lugo, Galicia, north-western Spain). Plot size was 12 × 8 m2, with a 1 m buffer strip between plots. Two-year data showed that fertilization with either material had a positive effect
on pasture production, with no significant difference between the two fertilization treatments. Tree growth in the milk sewage
sludge plot was significantly higher than in the control plots. Inorganic fertilization increased pasture production, but
affected tree growth negatively. The results show that milk sewage sludge could be used as a fertilizer in silvo-pastoral
systems.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献