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酶法提取香菇多糖新工艺研究 总被引：18，自引：0，他引：18
采用中性蛋白酶酶解法提取香菇中多糖．即在水提香菇多糖前．先用中性蛋白酶处理香菇粗粉，通过实验确定最佳的酶解工艺条件：酶解温度50℃，酶解pH4．8，酶解时间60min，酶与香菇粗粉配比为1．5：100。与传统工艺相比，提取率提高了40％以上．多糖中杂质蛋白质的含量降低了50％。 相似文献
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正交法优化三种灰树花多糖提取工艺 总被引：2，自引：0，他引：2
采用正交法,对传统热水浸提法,超声辅助水提法及微波辅助水提法的灰树花多糖提取工艺进行了研究。结果表明：热水浸提法的最佳工艺为pH值75,料水比为1：20,提取温度为100℃,提取时间为4h,醇沉浓度为95％。超声辅助热水授提法的最佳条件为：超声功率400W,超声时间6min,浸提时间2h,应用超声波可提高灰树花多糖提取率13．56％。微波辅助热水浸提法的最佳条件：高功率,微波时间4min,水提2h,可使灰树花多糖提取率升高29．52％。 相似文献
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水提法同步提取分离香菇中蛋白质和多糖的工艺研究 总被引：1，自引：0，他引：1
《食药用菌》2019,(5)
通过水提法同步提取分离香菇中的蛋白质和多糖,采用单因素实验研究料液比、提取温度、提取时间与蛋白质和多糖提取率的关系,并通过正交实验优化工艺条件。结果为,影响蛋白质提取率的主次因素为提取温度料液比提取时间;影响多糖提取率的主次因素为料液比提取温度提取时间。综合分析得到提取的最佳工艺组合为料液比20、提取温度100℃、提取时间3.5 h(二次提取)。该组合下蛋白质提取率为6.90%,多糖提取率达38.50%。 相似文献
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研究以采集自云南省楚雄州的青头菌、鸡油菌,云南省曲靖市马龙县的鸡菌为原材料,设计了正交试验探讨这三种野生食用菌子实体粗多糖的最佳提取方案,在此基础上比较了同一地区的不同野生食用菌子实体所含粗多糖含量的差别,不同地区的不同野生食用菌子实体多糖含量的差别。结果发现楚雄青头菌多糖最佳提取工艺为75℃浸提3 h,料液比为1∶40;楚雄鸡油菌最佳提取工艺为75℃浸提2.5 h,料液比1∶20;马龙鸡菌子实体多糖最佳提取工艺为80℃浸提2 h,料液比1∶20。楚雄青头菌子实体粗多糖提取率高于其他两种野生食用菌子实体粗多糖的提取率。 相似文献
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香菇子实体多糖分步酶解法提取研究 总被引：6，自引：1，他引：5
首先采用正交试验优化纤维素酶﹑果胶酶和木瓜蛋白酶对香菇(Lentinula edodes)子实体多糖酶解提取的工艺参数,然后在优化酶解条件下,依次采用纤维素酶﹑果胶酶和木瓜蛋白酶分步处理香菇子实体以提取香菇多糖,并与单一酶解提取法和传统热水浸提法进行对比.结果表明,纤维素酶﹑果胶酶﹑木瓜蛋白酶的最佳提取工艺参数依次为加酶量0.8%、温度45 ℃、pH 4.5、提取时间1 h,加酶量1.0%、温度45 ℃、pH 3.5、提取时间2.0 h和加酶量1.0%、温度45 ℃、pH 4.0、提取时间1.5 h;在优化提取条件下,分步酶解法提取香菇粗多糖的提取率可达14.17%,比传统热水浸提法提高128.2%,比单独采用纤维素酶﹑果胶酶﹑木瓜蛋白酶酶解提取分别提高了43.71%、46.99%和23.11%.紫外光谱分析表明,分步酶解法提取的香菇多糖纯度明显高于热水浸提法提取的香菇多糖.- 相似文献
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Transcatheter arterial chemoembolization (TACE) has become an important way to treat hepatocellular carcinoma (HCC). TACE can kill HCC cells directly. Cytokine-induced killer (CIK) cell therapy improves immunity. CIK sequential therapy combined with TACE improves treatment safety, efficacy, the quality of life and long-term outcome for the HCC patients. This review summarizes the status and latest progress of CIK cell therapy combined with TACE in the treatment of HCC. 相似文献
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The effects were studied of different concentrations of cycloheximide on the chemical deblossoming of mangoes. Very good results were obtained with 250 and 500 ppm and the panicles were completely killed within ten days of application. Before the old panicles were completely shed, new panicles which ultimately set fruits began to emerge. 相似文献
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野牛草属暖季型草坪草，质地柔软，草层厚密，耐践踏，具有耐旱、耐热、抗病虫害、适应性强等特点，管理粗放，年灌溉次数比较少，比冷季型草节约用水20％～40％，能够节约资金、节省水资源，在建设节约型园林绿化方面有突出的优势。野牛草的繁殖方式有：播种繁殖，草块、草皮繁殖。 相似文献
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对不同抗性甜瓜接种蔓枯病菌后测定叶片中叶绿素、可溶性糖、可溶性蛋白含量和3种防御酶（PPO、PO、PAL）的活性。结果表明：叶绿素含量在接种后呈现先增加后减小趋势,抗病甜瓜增幅大,叶绿素含量高于感病甜瓜;可溶性糖含量在接种后先降低后升高、再降低再升高,抗病甜瓜可溶性糖含量高于感病甜瓜;可溶性蛋白含量在接种后逐渐升高,感病甜瓜峰值出现早于抗病甜瓜;3种酶活性在接种后都有不同程度提高,其中抗病甜瓜酶活性增幅高于感病甜瓜。几种酶活性变化可以用于甜瓜蔓枯病抗性鉴定。 相似文献
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果园生草或稻（杂）草树盘覆盖具有调节果园微生态环境，防止水土流失，增强土壤通气性和透水性，保持旱季土壤湿度，提高土壤有机质，培肥地力，有利于害虫天敌生存活动及改善果实品质等作用。灌阳县现有李园近3333hm^2，绝大部分种植于山坡地，从夏末开始至秋冬季属于少雨干旱季节，此时正值李树果实迅速膨大期、成熟期和花芽分化期，若干旱因素引起李树供水、供肥不足，将影响到果实品质，花芽分化质量，形成大小年挂果，产量不稳定。近年来，笔者在桂林灌阳县的果园中进行了生草栽培、稻（杂）草覆盖栽培的试验，取得良好效果。 相似文献
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Protected horticultural crops as well as those planted in open fields particularly in the Mediterranean region have to cope with increasing salinization of irrigation water. High salinity of the supply water has detrimental effects on soil fertility and plant nutrition and reduces crop growth and yield. This study was conducted to determine if pre-inoculation of transplants with arbuscular mycorrhizal (AM) fungi alleviates salt effects on growth and yield of tomato (Lycopersicon esculentum Mill. Cv. Marriha) when irrigated with saline water. Tomato seeds were sown in polystyrene trays with 20 cm3 cells and treated with AM fungi (AM) or without (nonAM) Glomus mosseae. Once the seedlings were reached appropriate size, they were transplanted into nonsterile soil in concrete blocks (1.6 m × 3 m × 0.75 m) under greenhouse conditions. The soil electrical conductivity (ECe) was 1.4 dS m−1. Plants were irrigated with nonsaline water (ECw = 0.5 dS m−1) or saline water (ECw = 2.4 dS m−1) until harvest. These treatments resulted with soil EC at harvest 1.7 and 4.4 dS m−1 for nonsaline and saline water treatments, respectively. Root colonization with AM fungi at flowering was lower under saline than nonsaline conditions. Pre-inoculated tomato plants with AM fungi irrigated with both saline and nonsaline water had greater shoot and root dry matter (DM) yield and fruit fresh yield than nonAM plants. The enhancement in fruit fresh yield due to AM fungi inoculation was 29% under nonsaline and 60% under saline water conditions. Shoot contents of P, K, Zn, Cu, and Fe were higher in AM compared with nonAM plants grown under nonsaline and saline water conditions. Shoot Na concentrations were lower in AM than nonAM plants grown under saline water conditions. Results indicate that pre-inoculation of tomato transplants with AM fungi improved yield and can help alleviate deleterious effects of salt stress on crop yield. 相似文献
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施用ALA对不同棚龄蔬菜大棚番茄硝酸盐累积的影响 总被引：1，自引：0，他引：1
对3、5、7、10 a棚龄蔬菜大棚内的番茄喷施了不同浓度的ALA,结果表明:ALA对番茄果实内NO3--N、NO2--N的累积有较好的抑制效果,且长龄棚内的抑制效果最好,对NO2--N累积的抑制作用优于对NO-3-N的作用.试验范围内高浓度ALA的效果最佳. 相似文献