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1.
The interactive effect of rhizotrophic microorganisms on growth, yield, and nutrient uptake of wheat (Triticum aestivum L.) was determined in a pot experiment using sterilized soil deficient in available phosphorus (P). Positive effect on plant vigor, nutrient uptake, and yield in wheat plants was recorded in the treatment receiving mixed inoculum of nitrogen-fixing Azotobacter chroococcum + phosphate solubilizing microorganism (PSM) Pseudomonas striata + arbuscular mycorrhizal (AM) fungus Glomus fasciculatum. The available P status of the soil improved significantly (P ≤ 0.5) following triple inoculation with A. chroococcum, P. striata, and G. fasciculatum. The residual nitrogen (N) content of the soil did not change appreciably among the treatments. Addition of Penicillium variable to single- or double-inoculation treatments negatively affected the measured parameters. The population of A. chroococcum, PSM, percentage root infection, and spore density of the AM fungus in inoculated treatments increased at 80 days of wheat growth. The present finding showed that rhizotrophic microorganisms can interact positively in promoting plant growth, as well as N and P uptake, of wheat plants, leading to improved yield.  相似文献   

2.
Phosphorus (P)‐solubilizing bacteria and fungi can increase soil‐P availability, potentially enhancing crop yield when P is limiting. We studied the effectiveness of Bacillus FS‐3 and Aspergillus FS9 in enhancing strawberry (Fragaria × ananasa cv. Fern) yield and mineral content of leaves and fruits on a P‐deficient calcareous Aridisol in Eastern Anatolia, Turkey. The 120 d pot experiment was conducted in three replicates with three treatments (Bacillus FS‐3, Aspergillus FS9, control) and five increasing rates of P addition (0, 50, 100, 150, and 200 kg P ha–1). Fruit yield and nutrient content of fruits and leaves and soil P pools were determined at the end of the experiment. Phosphorus‐fertilizer addition increased all soil P fractions. Strawberry yield increased with P addition (quadratic function) reaching a maximum of 94 g pot–1 at 200 kg P ha–1 in the absence of P‐solubilizing microorganisms. At this yield level, Bacillus FS‐3 and Aspergillus FS9 inoculation resulted in P‐fertilizer savings of 149 kg P ha–1 and 102 kg P ha–1, respectively. Both microorganisms increased yields beyond the maximum achievable yield with sole P‐fertilizer addition. Microorganism inoculation increased fruit and leaf nutrient concentrations (N, P, K, Ca, and Fe) with the largest increases upon addition of Bacillus FS‐3. We conclude that Bacillus FS‐3 and Aspergillus FS9 show great promise as yield‐enhancing soil amendments in P‐deficient calcareous soils of Turkey. However, moderate additions of P fertilizer (50–100 kg ha–1) are required for highest yield.  相似文献   

3.
The efficiency of 13 phosphate-solubilizing bacteria (PSB; four Burkholderia sp., five Enterobacter sp., and four Bradyrhizobium sp.) was assessed in a soil plate assay by evaluating soil phosphorus (P) availability. A commercial argentine strain, Pseudomonas fluorescens, was used for comparing solubilizing activity. Burkholderia sp. PER2F, Enterobacter sp. PER3G, and Bradyrhizobium sp. PER2H strains solubilized the largest quantities of P in the soil plate assay after 60 days as compared with the other strains, including the commercial one. The effect of PSB inoculation on growth and nutrient uptake of soybean plants was also studied under greenhouse conditions. Plants inoculated with Burkholderia sp. PER2F had the highest aerial height and showed an appropriate N/P ratio. However, none of the PSB increased P uptake by plants. This suggests that PSB inoculation does not necessarily improve P nutrition in soybean, nor was there any relationship between P availability in the soil plate assay and P content in the soybean shoot in the greenhouse. We concluded that the selection of efficient PSB strains as possible inoculation tools for P-deficient soils should focus on the integral interpretation of soil assays, greenhouse experiments, and field trials.  相似文献   

4.
Phosphorus (P) limitation in soil is a major concern for crop productivity. However, the use of chemical fertilizer is hazardous to the environment and costly. Therefore, the use of phosphate-solubilizing bacteria (PSB) is an eco-friendly approach for a sustainable agricultural system. In the present study, a field trial was conducted for two consecutive years to study the effects of three PSB strains isolated, Bacillus licheniformis, Pantoea dispersa, and Staphylococcus sp., with different P fertilizer rates on P uptake, P use efficiency (PUE), and grain yield of rice. The activities of soil enzymes were also studied in relation to PSB treatments. Comparative analysis of the yield and biochemical parameters revealed that inoculation of PSB consortium could reduce almost 50% of the recommended P dose in rice cultivation. Three PSB strains in combination with 50% P dose was most effective and showed the highest increases in P uptake and PUE as compared to the uninoculated control. Moreover, the PSB consortium combined with 50% P dose contributed to 50.58% and 35.64% yield increases compared to the uninoculated control for 2018 and 2019, respectively. Significant increases in the activities of soil dehydrogenase, alkaline phosphatase, and acid phosphatase were also recorded under PSB treatment.  相似文献   

5.
《Applied soil ecology》2005,28(2):139-146
The effect of a combined inoculation of Rhizobium, a phosphate solubilizing Bacillus megaterium sub sp. phospaticum strain-PB and a biocontrol fungus Trichoderma spp. on growth, nutrient uptake and yield of chickpea were studied under glasshouse and field conditions. Combined inoculation of these three organisms showed increased germination, nutrient uptake, plant height, number of branches, nodulation, pea yield, and total biomass of chickpea compared to either individual inoculations or an uninoculated control. Increased growth and yield parameters were more pronounced when T. harzianum-PDBCTH 10 was inoculated along with the phosphate solubilizing bacterium and Rhizobium. Studies on population dynamics in the rhizosphere showed, there was no significant inhibition between the introduced organisms.  相似文献   

6.
Both earthworms and plant growth–promoting rhizobacteria (PGPR) are ubiquitous and important for promoting circulation of plant macronutrients. Two series of laboratory experiments were conducted to investigate the effects of earthworm casts and activities on the growth of PGPR, and the inoculation of earthworms and PGPR on the availability of N, P, and K in soils, respectively. During a short incubation period (0–34 h), the extracts of earthworm (Pheretima guillelmi)‐worked soil significantly (p < 0.05) increased the abundance of the three species of PGPR, including N‐fixing bacteria (NFB) (Azotobacter chroococcum HKN‐5), phosphate‐solubilizing bacteria (PSB) (Bacillus megaterium HKP‐1), and K‐solubilizing bacteria (KSB) (B. mucilaginous HKK‐1), in Luria‐Bertani (LB) broth. There were synergistic effects of dual inoculation of earthworms and PGPR on increasing the concentrations of NH$ _4^+ $ ‐N, (NO$ _3^- $ + NO$ _2^- $ )‐N, NaHCO3‐extractable P, and NH4OAc‐extractable K in the corresponding soils. Bioavailable N (the sum of NH$ _4^+ $ ‐N and [NO$ _3^- $ + NO$ _2^- $ ]‐N) in the dual inoculation was 4 to 24 times those inoculated with earthworms or NFB alone, respectively. The significantly higher concentrations of bioavailable N and P in the dual inoculation of earthworms and NFB or PSB may be due to the higher abundance of PGPR and/or higher activities of urease and acid phosphatase than those of single inoculation of NFB or PSB, respectively. Dual inoculation of earthworms and PGPR would be most effective in reducing the need for chemical fertilizers in agriculture.  相似文献   

7.
Natural and mutant strains of A. chroococcum were isolated from Indian soils. Their ability to dissolve phosphate and their phytohormone production were tested under in vitro conditions. In addition the effect of bacterial inoculation of Azotobacter on N, P, K uptake by three P responsive wheat genotypes (Triticum aestivum L.) under greenhouse conditions at five nutrient levels (Control, 90 kg N ha—1, 90 kg N + 26 kg P ha—1, 120 kg N ha—1 and 120 kg N + 26 kg P ha—1) was studied. In vitro phosphate solubilization and growth hormone production by mutant strains was more than by the soil isolates. Inoculation of wheat varieties with the soil isolates and mutant strains of A. chroococcum showed greater NPK uptakes as compared with parent soil isolates. Mutant strains M15 and M37 were proved to be the most effective for all three wheat varieties with regard to NPK uptake as well as root biomass production under greenhouse conditions.  相似文献   

8.
The effect of soil sterilization, and seed inoculation with three Rhizobium strains (3889, CP5b and IC 26) were studied on 5 chickpea (Cicer arietinum L.) genotypes (Jordan local, ILC 72 from Spain, ILC 484 from Turkey, C 235 from India, and ILC 1272 from U.S.A.). The main objective of the work was to investigate the effect of inoculation with different Rhizobium strains on yield, nodulation and other agronomic characteristics of different chickpea genotypes. Inoculation with Rhizobium resulted in a significant increase in grain yield for all genotypes tested. The average increase due to inoculation was 110% over the uninoculated control. Inoculation resulted in more nodules, greater nodule fresh weight and higher nitrogen uptake. The various Rhizobium strains differed in their effects. Genotypes responded differently to inoculation.  相似文献   

9.
A pot experiment in a greenhouse was conducted in order to investigate the effect of different N2‐fixing, phytohormone‐producing, and P‐solubilizing bacterial species on wheat and spinach growth and enzyme activities. Growth parameters and the activities of four enzymes, glucose‐6‐phosphate dehydrogenase (G6PD; EC 1.1.1.49), 6‐phosphogluconate dehydrogenase (6PGD; EC 1.1.1.44), glutathione reductase (GR; EC 1.8.1.7), and glutathione S‐transferase (GST; EC 2.5.1.18) were determined in the leaves of wheat (Triticum aestivum L., Konya) and spinach (Spinacia oleracea L.), noninoculated and inoculated with nine plant growth–promoting rhizobacteria (PGPR: Bacillus cereus RC18, Bacillus licheniformis RC08, Bacillus megaterium RC07, Bacillus subtilis RC11, Bacillus OSU‐142, Bacillus M‐13, Pseudomonas putida RC06, Paenibacillus polymyxa RC05 and RC14). Among the strains used in the present study, six PGPR exhibited nitrogenase activity and four were efficient in phosphate solubilization; all bacterial strains were efficient in indole acetic acid (IAA) production and significantly increased growth of wheat and spinach. Inoculation with PGPR increased wheat shoot fresh weight by 16.2%–53.8% and spinach shoot fresh weight by 2.2%–53.4% over control. PGPR inoculation gave leaf area increases by 6.0%–47.0% in wheat and 5.3%–49.3% in spinach. Inoculation increased plant height by 2.2%–24.6% and 1.9%–36.8% in wheat and spinach, respectively. A close relationship between plant growth and enzyme activities such as G6PD, 6PGD, GR, and GST was demonstrated. Plant‐growth response was variable and dependent on the inoculant strain, enzyme activity, plant species, and growth parameter evaluated. In particular, the N2‐fixing bacterial strains RC05, RC06, RC14, and OSU‐142 and the P‐solubilizing strains RC07 and RC08 have great potential in being formulated and used as biofertilizers.  相似文献   

10.
Influence of different phosphorus (P) sources on growth, yield and oil quality of linola was evaluated when randomized in complete block design using three replications. Treatments were control (No P), hydropriming, soil phosphorus (50 kg ha?1), seed inoculation with phosphate solubilizing bacteria (PSB, Bacillus spp.) and seed priming with single super phosphate (2%) alone and combined with reduced soil phosphorus (25 kg P ha?1). Among treatments, hydropriming and seed inoculation reduced seedling 50% and mean emergence time with highest emergence index, seedling fresh and dry weights and chlorophyll contents. Seed inoculation with soil P (25 kg ha?1) produced highest seeds per capsule, 100-seed weight, seed and biological yield, harvest index. Maximum oil percentage, low protein contents and high cost benefit ratio with net economic returns were also found for seed inoculation combined with soil phosphorus. Nonetheless, soil phosphorus application can be reduced when seed inoculation with PSB is employed.  相似文献   

11.
Improved nutrient‐use efficiency is important to sustain agricultural production. The goal of our study was to investigate the effects of Azovit® (Azotobacter chroococcum) inoculation of seed with N fertilization on crop yield, nutrient uptake, and N‐use efficiency (NUE) of irrigated cotton (Gossypium hirsutum L. cv. C‐6524) in secondary saline soil under continental climatic conditions of Uzbekistan. A randomized complete block design in a 4 × 2 split‐plot experiment was established in the fall of 2013. The main plot was N fertilization (0, 140, 210, and 280 kg ha?1) and the subplot was Azovit inoculation. Azovit inoculation consistently increased the seed and lint yields of cotton by 25 and 27.9%, respectively, at 210 kg N ha?1 compared to the respective control. Azovit with 210 kg N ha?1 significantly increased the cotton harvest index by 21%, when compared to the control. Likewise, nutrient uptake and NUE of cotton were higher when N (210 kg ha?1) was applied with Azovit, as compared to other treatment combinations. An extrapolation of the relationship of relative yield vs. N fertilization showed that Azovit at 210 kg N ha?1 was sufficient to obtain near‐maximum cotton production (90%) with highest NUE, as compared to the respective control. The results suggest that Azovit with 210 kg N ha?1 produces cotton yield higher and/or comparable with the currently used rates of 280 kg N ha?1 or higher, suggesting savings of 70 kg N ha?1 for cotton production in saline soils under continental climatic conditions.  相似文献   

12.
A field experiment was conducted at the G.B.Pant University Research Station,Ujhani(U.P.)in rainy (Kharif) season of the year 1994-1995 to study the effect of Rhizobium,VAM(vesicular arbuscular myc-orrhiza) and PSB(phophate solubilizing bacteria) inoculation,with and without P,on blackgram(Vigna mungo L.) seed yield.Phosphorus application in sol with medium P content(5.4 mg kg^-1) increased nodulation,grain yield,N and P in plant and grain over no phosphorus control.Forty kilograms of P2O5 each hactare recorded an increase of 20.6% in nodule dry weight,significant increases of 0.35 g kg^-1 in N concentration and 1.28 g kg^-1 in P concentration of plant over 20 kg P2O5 ha^-1 ,Similar significan increases of 0.5 g kg^-1 in grain yield and 0.54 and 0.23 g kg^-1 in N and P concentrations of the grain,respectively,over 20 kg P2O5 ha^-1 were also obtained with higher dose.Inoculation of Rhizobium VAM PSB at all the stages of plant growth recorded maximum increaes in all the parameers studied .Dual inoculation of Rhizobium with either VAM or PSB was generally significan in the effect and better than that of VAM PSB,however,P accumulation in plat and grain was more with VAM PSB.A mong single inoculations,Rhizobium gave highest and 21.0% more nodule number,34.7% more nodule dry mass,0.73 g kg^-1 more N in grain and 4.2% higher grain yield over PSB.PSB.however,registered significant increases in P concentration in plant and grain over VAM and Rhizobium.  相似文献   

13.
Plant growth-promoting bacteria (PGPB) Pseudomonas lurida-NPRp15 and Pseudomonas putida-PGRs4 possessing multiple plant growth-promoting traits were isolated from rhizoplane of pea and rhizosphere of garlic, respectively. The effects of individuals and combinations of Pseudomonas spp. with effective root nodulating symbiotic nitrogen fixing Rhizobium leguminosarum-FB1 on plant growth, nutrient uptake and yield of the rajmash plant were studied under greenhouse conditions. Bacterial inoculation resulted in significantly higher values for plant dry biomass, N, P, K, Zn and Fe contents as compared to the uninoculated control. Furthermore, dual inoculation of P. lurida-NPRp15 with R. leguminosarum-FB1 significantly increased root and shoot dry weight, nodulation, nutrient uptake, pod yield, and nutrient content of pods of rajmash VL63 compared to controls, single and triple inoculation. The results of the study indicate the potential of harnessing the benefit of plant growth-promoting and nitrogen-fixing microorganisms to improve the growth and yield of rajmash.  相似文献   

14.
The objectives of this work were to isolate and characterize walnut phosphate-solubilizing bacteria (PSB) and to evaluate the effect of inoculation with the selected PSB stains to walnut seedlings fertilized with or without insoluble phosphate. Thirty-four PSB strains were isolated and identified under the genera Pseudomonas, Stenotrophomonas, Bacillus, Cupriavidus, Agrobacterium, Acinetobacter, Arthrobacter, Pantoea, and Rhodococcus through a comparison of the 16S ribosomal DNA sequences. All isolated PSB strains could solubilize tricalcium phosphate (TCP) in solid and liquid media. Phosphate-solubilizing activity of these strains was associated with a drop in the pH of medium. A significantly negative linear correlation was found between culture pH and phosphorus (P) solubilized from inorganic phosphate. Three isolates Pseudomonas chlororaphis (W24), Bacillus cereus (W9), and Pseudomonas fluorescens (W12) were selected for shade house assays because of their higher phosphate-solubilizing abilities. Under shade house conditions, application of W24 or W12 remarkably improved plant height, shoot and root dry weight, and P and nitrogen (N) uptake of walnut seedlings. These increases were higher on combined inoculation of PSB with TCP addition. The most pronounced beneficial effect on growth of walnut plants was observed in the co-inoculation of the three PSB strains with TCP addition. In comparison, the isolate of W9 failed to increase available soil P, nutrient levels in plants, or to promote plant growth, suggesting that more insoluble phosphate compounds than tricalcium phosphate should be used as substrates to assess the phosphate-solubilizing ability of PSB under greenhouse conditions. The present results indicated that strains P. chlororaphis or P. fluorescens could be considered for the formulation of new inoculants of walnut, even of more woody plants.  相似文献   

15.
Drawbacks of intensive farming practices and environmental costs of N fertilizers have renewed interest in bio‐fertilizers. This study was conducted in order to investigate the effectiveness of 7 N2‐fixing bacterial isolates from various sources in sugar beet and barley production under field conditions in the higland plateau of Erzurum, Turkey (29° 55′ N and 41° 16′ E with an altitude of 1950 m) in 1999 and 2000. Seeds were inoculated with five bacterial strains of Bacillus; BA‐140, BA‐142, M‐3, M‐13, and M‐58, a strain of Burkholderia (BA‐7) and Pseudomonas (BA‐8). The bacterial strains had been demonstrated to grow in N‐free basal medium. The experiment also included applications of mineral nitrogen (N), phosphorous (P), and NP‐fertilizer as well as a control treatment without inoculation and fertilizer application. Two years of trials under field conditions showed that seed inoculation with bacterial strains significantly affected yield, yield components, and quality parameters both in sugar beet and barley. On an average of both years, seed inoculation of sugar beet with BA‐140, BA‐142, M‐58, BA‐7, BA‐8, M‐13, and M‐3 increased root yield by 13.0, 12.6, 10.5, 9.2, 8.1, 6.1, and 6.5% as compared to the control and sugar yield by 7.8, 6.3, 5.1, 4.0, 3.2, 2.3, and 5.3%, respectively. N, P, and NP applications, however, increased root yield up to 13.6, 5.3, and 21.4% and sugar yield by 6.1, 4.0, and 14.8%, respectively. Of the bacteria tested, BA‐140 and BA‐142 had yields equal to N application. All bacterial inoculations also gave higher seed and total biomass yields in barley than control plots. BA‐140 and BA‐142 were top yielding strains. In conclusion, bacterial seed inoculations especially with BA‐140 and BA‐142 may satisfy nitrogen requirements of sugar beet and barley under field conditions even in upland areas.  相似文献   

16.
G. KAUR  M. S. REDDY 《土壤圈》2015,25(3):428-437
A two-year field study was conducted to test the effects of two phosphate-solubilizing bacteria (PSB), Pantoea cypripedii (PSB-3) and Pseudomonas plecoglossicida (PSB-5), inoculated singly or together with rock phosphate (RP) fertilization on maize and wheat cropping cycle by comparing with chemical P fertilizer (diammonium phosphate, DAP), mainly in the crop yield, soil fertility and economic returns. Inoculation of PSB together with RP fertilization increased the crop growth in terms of shoot height, shoot and root dry biomass, grain yield and total P uptake in both maize and wheat crops compared to the other treatments. Soil fertility in the context of available P, enzyme activities and PSB population in both maize and wheat crops was significantly improved with PSB inoculation together with RP fertilization compared to DAP treatment. The combined use of PSB inoculation and RP fertilization was more economical due to minimal cost and maximum returns. These results suggested that PSB inoculation along with RP fertilization would be an appropriate substitute for chemical phosphate fertilizer application in sustainable agriculture systems.  相似文献   

17.
The effects of phosphate solubilizing bacteria (Bacillus megateriumi) (PSB) and earthworms (Pheretima guillelmi and Eisenia fetida) on phosphorus (P) turnover and transformation in soil were investigated in a laboratory experiment lasting for 21 days. The treatments included soil + P. guillelmi (SW1), soil + E. fetida (SW2), soil + B. megaterium (SB), soil + P. guillelmi + B. megaterium (SBW1), soil + E. fetida + B. megaterium (SBW2), and the control with sterilized soil (control) only. The results showed that the number of B. megaterium. was enhanced in all treatments especially those with earthworms added when compared with the control. Activities of acid phosphatase (pH = 6.5) increased in all treatments, especially in the presence of P. guillelmi with or without PSB (64 % and 38 %, respectively). Significant increases in both inorganic P and water‐soluble P were observed in treatments involving B. megaterium. and earthworms when compared with the control. Inoculation of both earthworms and PSB had significant effects on microbial growth, enzymatic activity, and thus enhanced the release of available P. The dual inoculation of earthworms and bacteria further accelerated P transformation. Different performances observed for the earthworm species were probably due to their different feeding habits and physiology.  相似文献   

18.
The use of phosphate-solubilising bacteria as inoculants increases plant phosphorus (P) uptake and thus crop yield. Strains from the genus Mesorhizobium are among the most powerful phosphate solubilizing microorganisms. In order to study efficiency in P uptake and N2 fixation in chickpea (Cicer aritenium), forty-two rhizobia strains natively from Tunisian soils were studied in symbiosis with the chickpea variety “Béja1” which is frequently cultivated in Tunisia. Plants were inoculated separately with these strains under controlled conditions in perlite under two sources of P i.e. soluble (KH2PO4) and insoluble P (Ca2HPO4). At flowering stage, growth, nodulation, P uptake and N2 fixation were assessed in all symbiotic combinations. The results showed that the S27 strain efficiently mobilized P into plants, observed as a significant increase of plant P content when insoluble P (Ca2HPO4) was supplied to the soil. This was associated with a significant increase in plant biomass, nodule number and N content under insoluble P conditions. Additionally, inoculation with the Mesorhizobium strain S27 significantly increased the root acid phosphatase activity under insoluble P. This study also shows significant correlations found between plant P content and acid phosphatase activity under low P conditions which may highlight the contribution of acid phosphatases in increasing P use efficiency. A field experiment also showed that most of the chickpea analyzed parameters were improved when plants inoculated with two selected rhizobia strains (S26 and S27) and supplied with P2O5. Overall, these findings postulate that rhizobial inoculation should not only be based on the effectiveness of strains regarding N fixation, but also to other traits such as P solubilisation potential.  相似文献   

19.
Effects of Glycine–Glomus–phosphate solubilizing bacteria (PSB) interactions were studied on productivity, nutrient dynamics, and root colonization in soybean in a phosphorus (P)–deficient Himalayan acidic Alfisol in a greenhouse experiment. Treatments consisted of three vesicular arbuscular mycorrhizae (VAM) cultures, VAML [VAM culture, Glomus mosseae, developed by CSK Himachal Pradesh Agricultural University, Palampur, India], VAMT [VAM culture, Glomus intraradices, developed by Centre for Mycorrhizal Research, The Energy and Resources Institute (TERI), New Delhi, India], and VAMI [VAM culture, Glomus mosseae, developed by Indian Agricultural Research Institute (IARI), New Delhi, India], and a local PSB culture (Pseudomonas striata) alone or in combination with or without 75% of recommended phosphorus pentoxide (P2O5) dose based on targeted yield concept following the soil-test crop response (STCR) precision model. Sole application of PSB or either of the above VAM cultures considerably enhanced VA-mycorrhizal root colonization and root weight besides crop productivity and nutrient uptake over control. A similar stimulatory effect with significant enhancement on mycorrhizal root colonization and root weight was observed with coinoculation of PSB and VAM cultures over the control. Dual inoculation of VAM and PSB cultures also resulted in significant improvement in grain and straw yield besides grain protein content, thereby revealing a synergistic interaction between VAM and PSB. Coinoculation with either of VAMT (Glomus intraradices) or VAMI (Glomus mosseae) + PSB + 75% P2O5 dose remained at par with sole application of 100% P2O5 dose with respect to crop productivity, nutrient content, nutrient uptake, and soil fertility status besides the greatest root colonization and root weight at flowering, indicating that Glycine–Glomus–PSB interactions in combination with 75% P2O5 dose based on STCR precision model lead to economization of fertilizer P by about one-fourth without impairing crop productivity and soil fertility in soybean in a Himalayan acidic Alfisol.  相似文献   

20.
Abstract

Zinc (Zn) fertilization in rice is important to enhance productivity and increase Zn concentration in rice grain to improve its nutritional status. A field experiment was conducted in wet seasons of 2013 and 2014 to study Zn nutrition of rice in three different crop establishment methods (CEMs) viz. puddled transplanted rice (PTR), system of rice intensification (SRI) and aerobic rice system (ARS), under three different rates of nitrogen (N) and phosphorus (P) viz. 0, 75 and 100% of recommended dose of fertilizer (RDF) (120?kg N ha?1 and 25.8?kg P ha?1) and two different sources of N and P viz. chemical fertilizer and microbial inoculation (MI). Concentration and uptake of Zn at different growth stages and in straw and milled rice was significantly higher in PTR and SRI than ARS. Soil DTPA–extractable Zn content of soil was increased by 1142.4, 1140.3 and 755.8?g ha?1 in PTR, SRI and ARS after two year of Zn fertilization (soil application of 5?kg Zn ha?1). Zinc nutrition increase its Zn concentration in straw and milled rice and improvement in total uptake was 38.1, 40.3 and 40.8?g ha?1 when Zn was applied with RDF, 75% RDF + Anabaena sp. (CR1) + Providencia sp (PR3) consortia (MI1) and 75% RDF + Anabaena-Pseudomonas biofilmed bio-fertilizer (MI2), respectively. Positive correlation between milled rice yield and Zn concentration (R2= 0.95 and 0.97) showed the importance of Zn nutrition in improving rice yield. Zinc concentration at 70?days after sowing (DAS) and 100 DAS was also found positively correlated with dehydrogenase activity and microbial biomass carbon in soil.  相似文献   

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