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为了研究不同年龄、不同性别间麋鹿生长激素的变化水平,应用酶联免疫法测定了79只健康麋鹿血清生长激素水平.结果表明,年龄和性别不同的群体,血清生长激素分泌水平有所不同.麋鹿1岁时生长激素分泌最高,数值最大的个体也出现在1岁,值为1.52 μg/L,说明从整体上来看麋鹿1岁时生长最为旺盛.雄性生长激素分泌均值高于雌性,雄性生长激素平均值为0.6321 μg/L±0.06151 μg/L,雌性为0.6158μg/L±0.05743 μg/L,生长激素含量在1岁与2岁间存在极显著性差异(P＜0.01).说明年龄和性别是影响血清生长激素分泌的因素. 相似文献
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旨在研究麋鹿(Elaphurus davidianus)血浆激素水平的季节性变化。2016年4月至2017年3月对江苏省大丰麋鹿国家级自然保护区的96头麋鹿(48雄、48雌)采集血液样本,用放射性免疫分析(RIA)测定了麋鹿血浆激素水平。结果表明,雄性麋鹿促卵泡素(FSH)、促黄体素(LH)和促乳素(Pr)数值均较低,睾酮(T)数值在6月份明显大于其他月份;雌性麋鹿Pr数值4月份最高,7月份最低,雌二醇(E2)数值从6月份开始升高,孕酮(P4)数值从7月开始升高,T数值都较低;经Mann-Whitney-U检验雌、雄麋鹿,P4、E2和T水平存在显著差异(P0.01);E2呈现显著正相关(P0.01),P4和T水平呈现显著负相关(P0.01);雄性麋鹿6项性激素指标两两比较,相关性均不显著(P0.05),但E2和T水平与其他4项激素水平都存在负相关;雌性麋鹿6项性激素指标之间比较,相关性都不显著(P0.05),但Pr和T水平与其他4项激素水平都存在负相关。试验结果为麋鹿生殖生理研究与利用提供了基础资料。 相似文献
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北京南海子麋鹿血液生理生化指标的测定 总被引：2，自引：1，他引：1
为了解麋鹿的健康状态,给疾病临床诊断和治疗提供依据,对北京南海子临床健康麋鹿的血液生理生化指标进行了测定.其中11只麋鹿测定了14项血液生理指标,32只麋鹿测定了35项血液生化指标.不同性别指标值之间的差异以及亚成体和成体之间生化值的差异,使用Mann-Whitney U检验进行了对比.结果显示,血液生理指标值除血小板计数、血小板压积和血小板体积分布宽度指标上雄性显著高于雌性,其他指标雌雄间差异不显著;不同性别之间血清生化指标值仅乳酸脱氢酶、二氧化碳、血清K+等指标差异显著,其他值雌雄间差异不显著;麋鹿成体与亚成体之间在乳酸脱氢酶、天冬氨酸氨基转移酶、羟丁酸脱氢酶、丙氨酸氨基转移酶、碱性磷酸酶、肌酸同工酶、白球比、总蛋白定量和球蛋白等多项指标差异较显著.与以往研究相比,本次新增加了16项麋鹿血液生理生化指标值的测定,测定项目中总胆红素和尿酸测定值与以往值存在显著差异. 相似文献
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本试验旨在探讨不同储存期玉米对肉鸡免疫功能的影响。选取1日龄雌性科宝商品代雏鸡144只,根据体重均衡原则分成3组(每组8个重复,每个重复6只),分别饲喂由储存2、4年的玉米配制的试验饲粮,与储存0年的玉米作对照,试验期42 d,测定17日龄时血液淋巴细胞转化率,21、42日龄时免疫器官指数、血清免疫球蛋白G含量、血清新城疫抗体效价和血清溶菌酶活性指标。结果表明:1)21日龄时,与对照组相比,储存2年的玉米对肉鸡免疫器官指数、血液特异性免疫和非特异性免疫均没有显著影响(P>0.05),但储存4年的玉米显著降低了肉鸡胸腺指数、法氏囊指数和脾脏指数(P<0.05),显著降低了淋巴细胞对伴刀豆球蛋白A的反应性(P<0.05),以及血清免疫球蛋白G含量、新城疫抗体效价和溶菌酶活性(P<0.05)。2)42日龄时,与对照组相比,储存2年的玉米对肉鸡免疫器官指数、血液特异性免疫和非特异性免疫也均没有显著影响(P>0.05),但储存4年的玉米显著降低了肉鸡脾脏指数和血清免疫球蛋白G含量(P<0.05),对其他免疫指标没有显著影响(P>0.05)。结果显示,玉米储存期过长可导致肉鸡的免疫功能下降。 相似文献
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为了解山东省规模猪场的猪瘟免疫效果及变化趋势,2011—2019年在16个地市717个规模猪场,采集血清样品22 465份,采用猪瘟抗体ELISA方法进行抗体检测,并比对不同年份和不同场点的免疫水平。结果显示:2011—2019年,全省16个地市种猪场的场群免疫合格率为73.84%,个体合格率为77.54%;商品代猪场的场群免疫合格率为63.96%,个体免疫合格率为71.79%。从不同年份看,规模猪场的猪瘟免疫抗体水平呈波浪状变化,2011—2012年免疫水平较高,2013—2014年较低,2015—2016年再次升高,2017—2019年免疫水平又出现下降;从不同场点看,无论是个体免疫效果,还是群体免疫效果,种猪场均优于商品代猪场。结果表明,山东省规模猪场的总体猪瘟免疫抗体水平不高且不稳定,因而规模猪场应持续加强猪瘟的免疫和监测,尤其是商品代猪群,以降低猪瘟的发生风险。 相似文献
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《中国兽医杂志》2019,(1)
为探究川续断水煎剂对小鼠血清中免疫球蛋白(lmmunoglobulim,lg)和细胞因子的影响,评估其对动物机体免疫功能的调节作用。将40只昆明系健康小鼠,雌雄各半,随机分为5组,每组8只,分别按体质量灌胃10 g/kg·bw、20 g/kg·bw、30 g/kg·bw、40 g/kg·bw终浓度为2 g/mL的川续断水煎剂和生理盐水,连续给药7 d。于末次给药后第7天无菌操作摘除眼球取血分离血清,测定小鼠血清中免疫球蛋白和细胞因子水平。结果显示,与对照组相比,灌服不同剂量的川续断水煎剂均能显著提高小鼠血清中IgA和IgM水平(P<0.05),不同程度地提高IgG的水平;显著升高小鼠血清中IL-2、INF-γ和TNF-α水平(P<0.05),显著降低IL-4和IL-10水平(P<0.05)。表明川续断水煎剂可升高小鼠血清中免疫球蛋白水平,同时能增强血清中Th1类细胞因子水平,降低Th2类细胞因子的水平,提示川续断可增强动物机体的非特异性免疫功能。 相似文献
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麋鹿(Elaphurus Davidianus)是中国特有物种,野生种群在本土灭绝近200年,2018年大丰麋鹿国家级自然保护区开展了小麋鹿的人工驯养试验,通过合理饮食饲喂与建立条件反射、食物诱导强化训练等方式相结合,在试验场地对刚出生的小麋鹿个体开展驯养试验,总结了麋鹿驯养中饲养的日粮配比和成功驯化的技术方案,探讨了麋鹿人工驯养的关键措施和技术难点,为进一步开展人工驯养麋鹿提供技术参考和经验借鉴。 相似文献
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试验将40只7日龄雏鸡分为3组,分别每日经口灌服不同浓度砷溶液和生理盐水,分别于8、10、14、17日龄测定血液红细胞免疫功能及血清中免疫球蛋白含量.结果显示,不同浓度的砷溶液均可以导致雏鸡RBC-C3bR花环率先升高(P＞0.05)再降低(P＜0.01或P＜0.05),RBC-IC花环率先降低(P＞0.05)再升高(P＜0.01或P＜0.05),血液中免疫球蛋白含量也是先微升高(P＞0.05)再降低(P＜0.01或P＜0.05),且呈时间剂量效应.表明砷可影响雏鸡红细胞免疫功能及血清中免疫球蛋白的含量,降低雏鸡血液的免疫功能. 相似文献
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Masami Numata Takashi Kondo Yasuo Nambo Yasunaga Yoshikawa Kiyotaka Watanabe Koichi Orino 《Animal Science Journal》2013,84(12):782-789
Antibody (immunoglobulin G (IgG), IgM or IgA) levels relative to ferritin in six foal sera (three male and three female) after birth (day 0 and 2, 6, 10, 20, 28, 36, 40, 52 and 56 weeks of age) were semi‐quantitatively measured with normalization with antibody activity to ferritin in one adult horse serum. After addition of horse spleen ferritin to the serum sample, the complex formed between antibodies to ferritin in the serum and ferritin was co‐immunoprecipitated using antibody to horse spleen ferritin. Antibody classes of the co‐immnoprecipitate were detected with antibodies specific for horse IgG, IgM or IgA heavy chain. Six adult horse serum samples were found to have ferritin‐binding activities in all immunoglobulin classes examined. Although ferritin antibody activities (IgG, IgM and IgA) were scant in the foal sera before sucking colostrum (day 0), their activities increased at 2 weeks of age. IgG antibodies showed a biphasic response and IgM antibody activity increased up to 40 weeks of age. Antibody (IgG, IgM and IgA) activities to ferritin in three colostrum samples were significantly higher than in adult horse serum samples. These results demonstrate that antibody to ferritin in foal serum is derived from colostrum after birth and is produced thereafter. 相似文献
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Guidry J Butler JE Pearson RE Weinland BT 《Veterinary immunology and immunopathology》1980,1(4):329-341
Bovine IgG1, IgG2, IgA, and IgM were measured in the serum and lacteal secretions of six cows from 10 days prepartum to 240 days of lactation. Immunoglobulins in lacteal secretions were expressed in units of concentration (mg/ml) as well as in total daily output. All isotypes were selectively accumulated during colostrum formation. The rate of IgG1 accumulation decreased rapidly after calving; this decrease corresponded to a return to normal serum levels of this immunoglobulin. Selective accumulation of IgA > IgM > IgG1 was maintained throughout lactation, but IgG2 showed no selective accumulation beyond 5 days postpartum. In serum, IgA and IgM levels were elevated at parturition and showed a significant decrease postpartum. Increases in serum IgA levels 60 days postpartum corresponded to a rise in lacteal concentration. The concentration of all immunoglobulins increased during late lactation, coincident with a major reduction in milk yield. Six strains of mastitis-causing organisms were cultured during the period of the experiment; however, none resulted in clinical mastitis or showed an effect on immunoglobulin secretion. 相似文献
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Immunoglobulins were quantitated by single radial immunodiffusion in 34 female Holstein-Friesian calves which had been kept with their dams for the first 24 hours of life. The mean immunoglobulin G1 (IgG1) concentration was 1.063 g/dl; IgG2, 0.093 g/dl; IgM, 0.171 g/dl; and IgA, 0.125 g/dl. Both serum total protein as measured by refractometer and zinc sulfate turbidity measured at 498 nm gave good correlations with total protein, which were significant, P less than 0.001. Plasma total protein had a slightly poorer correlation with total immunoglobulins, presumably due to variable fibrinogen content. Plasma total protein gave a better correlation with total immunoglobulins than did any of the immunoglobulin classes individually. Total protein by refractometer underestimated naturally occurring or added immunoglobulins by one-third. 相似文献
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The salivary and serum concentrations of immunoglobulins G, M and A (IgG, IgM and IgA), and the salivary concentrations of albumin were measured by ELISA in 30 cats with chronic gingivostomatitis and 32 healthy cats. The cats with chronic gingivostomatitis had significantly higher salivary concentrations of IgG, IgM and albumin, and higher serum concentrations of IgG, IgM and IgA, but significantly lower salivary concentrations of IgA than the healthy cats. The cats with chronic gingivostomatitis were treated with either methylprednisolone, sodium aurothiomalate, metronidazole and spiramycin, or oral hygiene products. After three months of treatment, the cats receiving methylprednisolone had a significant reduction in serum IgG levels compared to the cats treated with sodium aurothiomalate or metronidazole and spiramycin, but after six months of treatment there were no significant differences between the groups. Before the treatments, the levels of oral inflammation were not correlated significantly with any of the serum or salivary immunoglobulin levels. However, the changes in oral inflammation were correlated significantly with the changes in the salivary IgM concentration after three and six months of treatment, and with the change in the salivary IgA concentration after six months of treatment. 相似文献
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F. Klobasa F. Habe E. Werhahn J.E. Butler 《Veterinary immunology and immunopathology》1985,10(4):341-353
The concentrations of IgG, IgM and IgA in sera collected from 3855 sows (3208 pregnant and 647 lactating) at a single time point were determined. This experimental design allowed changes in serum immunoglobulin over the reproductive cycle to be studied without bias from seasonal influence. The concentrations of the three immunoglobulins changed independently during the reproductive cycle. Serum levels of IgM and IgG began a progressive postpartal decline during the 14th–17th week of gestation. At the onset of lactation serum IgG levels progressively increased while IgM levels continued to decline, the latter reaching their lowest level during the third week of lactation. In contrast to IgM and IgG, serum IgA levels increased 35% during weeks 14–17 of gestation and continued to increase throughout lactation, reaching their highest serum levels in the third week of lactation; the serum IgA concentration at this time was twice that observed during the first 13 weeks of gestation. Results of these studies allowed the reproductive cycle to be classified into four phases on the basis of serum immunoglobulin concentrations: (1) weeks 1–4 of gestation; (2) weeks 5–13 of gestation; (3) weeks 14–17 of gestation and (4) lactation. 相似文献
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A quantitative competitive binding "triple-sandwich" enzyme immunoassay was developed and used to evaluated pathogen/class-specific antibody responses in Holstein-Friesian cows vaccinated against Clostridium perfringens B-toxin. Vaccination of cows at six weeks and again at two weeks prepartum increased pathogen-specific IgG levels in each dam's colostrum and respective calf's serum. Pathogen-specific IgG and IgM concentrations in dams' sera and colostra were related to subsequent pathogen-specific IgG and IgM neonatal sera concentrations. Only pathogen-specific IgA in dams' colostra was correlated to neonatal levels, possibly owing to a different origin and role of this immunoglobulin class. All class-specific colostral immunoglobulin levels were related to subsequent neonatal concentrations. Isotypic antibody responses against C. perfringens B-toxin were found with pathogen-specific IgM predominant in dams' sera and pathogen-specific IgA predominant in colostra and neonatal sera. 相似文献
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A total of 103 ewes from the breeds Black mutt., Texel, Finn. L., Heidschnucke and the crossbreeds Texel x Finn. L. and Black. mutt. x Finn. L. was studied. Blood samples were drawn at days 1, 7, 21 and 42 and milk samples at the 4th, 12th, 24th and 72nd hour after the onset of lactation and subsequently on days 7, 21 and 42. The concentrations of the immunoglobulins IgG1, IgG2, IgM and IgA were assayed in serum and milk. The following results were obtained: 1. The total immunoglobulin contents in the serum was not significantly different between breeds. 2. All ewes showed a rise in serum immunoglobulin concentrations by about one third over the first six weeks of lactation. Between 50-60% of this increase were on the account of IgG1. 3. The serum concentration of IgG1 and IgG1 rose as of the third day, those of IgM as of day 21 after lambing. 4. The rise in serum immunoglobulin concentration continued after the weaning of the lambs. 5. The ratio of IgG1 to IgG1 in ewe serum was 2:1. 6. The immunoglobulin concentration in milk dropped sharply on the first day of lactation, followed by a continuous, more gradual decrease over the entire course of lactation. A terminal rise, as observed in sows, could not be detected. 7. The ratio of IgG1:IgG2 : IgM : IgA in the whey changed from 85 : 1 : 12 : 2 on day one to 70 : 7 : 12 : 11 on the last day of lactation. 8. While characteristic trends in immunoglobulin patterns in the sera of ewes over the course of lactation are clearly discernible, it is not possible to denote "normal" values. 相似文献
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Samples of urine and serum from 45 newborn rottweiler puppies from six litters, and milk from their mothers, were taken 24, 48 and 72 hours and seven and 14 days after birth. Urine total protein and creatinine concentrations were determined and the ratios calculated. The immunoglobulin (Ig) concentrations of IgG, IgM and IgA in urine, serum and milk were determined with a commercially available elisa kit. The concentration of total protein in urine decreased from 1.64 to 0.29 mg/ml, and it and the ratio of total protein to creatinine in the urine of the neonatal puppies exceeded the normal values for adult dogs, but all the puppies developed normally. The average concentration of IgG in urine decreased from 0.0035 to 0.0003 mg/ml, that of IgA from 0.0035 to 0.0002 mg/ml and that of IgM from 0.0006 mg/ml to undetectable levels after two weeks. After two weeks, 47 per cent of the puppies had measurable levels of IgA and 70.6 per cent had measurable levels of IgG, but none of them had measurable levels of IgM. 相似文献
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The levels of albumin, immunoglobulin G (including IgG [T]), IgA and IgM in the serum and aqueous humour of 47 horses and ponies with no ophthalmic abnormalities were determined by single radial immunodiffusion. There was a linear relationship between serum and aqueous albumin levels. No relationship of serum and aqueous IgG levels was observed. IgA was detected in only one aqueous sample. IgM was not detected in aqueous humour from any eye in this study. The factors which may influence albumin and immunoglobulin levels in normal aqueous humour are discussed. 相似文献
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Survey of serum IgA, IgG, and IgM concentrations in a large beagle population in which IgA deficiency had been identified 总被引：2，自引：0，他引：2
L T Glickman F S Shofer A J Payton L L Laster P J Felsburg 《American journal of veterinary research》1988,49(8):1240-1245
Concentrations of serum IgA, IgG, and IgM were determined for 829 adult Beagles from a commercial kennel in which several IgA-deficient dogs had been identified previously (index kennel). These values were compared with measurements of 100 adult dogs from another Beagle kennel (control kennel). After adjustment for differences in the ages and gender of the dogs, dogs from the index kennel had significantly (P less than 0.0001) lower IgA concentrations (mean, 46 mg/dl) than dogs from the control kennel (mean, 68 mg/dl). Regardless of kennel, males had significantly (P less than 0.01) higher IgA concentrations than did females. Dogs in the control kennel had significantly (P less than 0.04) higher IgG concentrations (mean, 2,649 mg/dl) than did dogs in the index kennel (mean, 2,478 mg/dl), and female dogs in the control kennel had significantly (P less than 0.05) higher IgM concentrations (mean, 189 mg/dl) than dogs of either sex in the index kennel (mean, 162 mg/dl) or male dogs in the control kennel (mean, 163 mg/dl). For both sexes, concentrations of IgA, IgG, and IgM increased with age. 相似文献