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1.
鸡传染性喉气管炎病毒SA2疫苗株免疫效力的研究 总被引：1，自引：0，他引：1
鸡传染性喉气管炎病毒（ＩＬＴＶ）疫苗株尿囊膜乳剂原液经点眼接种２、７和１５日龄雏，２日龄雏鸡组在接种后第７天有２０％（１／５）鸡出现眼睑肿胀和流泪等眼部反应，７日龄和１５日龄雏鸡组无明显临床变化。用该疫苗株乳剂原液点眼接种１月龄以上鸡眼部无明显反应，但通过气管接种后，２６．７％（４／１５）鸡表现出ＩＬＴ临床症状。试验用ＳＡ２株点眼接种免疫１月龄以上鸡，免疫组保护率为１００％（２０／２０），对照组发病率为１００％（１０／１０）、死亡率为４０％（４／１０）。２、７和１５日龄雏鸡点眼毒力试验的３组鸡，于接种后２１～３４天用王岗株ＩＬＴ强毒攻击，１００％得到保护，而对照组鸡１００％发病。试验结果表明，ＳＡ２疫苗株是一株毒力温和、副作用小而且免疫效力好的疫苗用毒株 相似文献
2.
本实验目的在于探讨不同毒株鸡传染性支气管炎病毒和鸡新城疫病毒混合接种同一鸡胚尿囊腔培养时二者之间的相互作用,分别将按２００倍,５００倍,１０００倍不同浓度稀释的鸡传染性支气管炎病毒Ｆ３株,肾型Ｊ株,Ｈ１２０,２８／８６株,Ｈ５２和Ｈ１２０株的１００倍稀释的Ｌａｓｏｔａ鸡新城疫病毒联合接种到１０日龄鸡胚尿囊腔,同时设单独培养作城疫病毒效价。结果表明,鸡新城疫病毒对鸡传染性支气管炎病毒增殖无明显促进作 相似文献
3.
本实验目的在于探讨不同毒株鸡传染性支气管炎病毒和鸡新城疫病毒混合接种同一鸡胚尿囊腔培养时二者之间的相互作用。分别将按２００倍、５００倍、１０００倍不同浓度稀释的鸡传染性支气管炎病毒Ｆ３株、肾型Ｊ株、Ｈ１２０２８／８６株,Ｈ５２和Ｈ１２０株和１００倍稀释的Ｌａｓｏｔａ鸡新城疫病毒联合接种到１０日龄鸡胚尿囊腔,同时设单独培养作对照,９６小时后收取尿囊液,用对流免疫电泳法检测鸡传染性支气管炎病毒效价,用血球凝集试验检测尿囊液鸡新城疫病毒效价。结果表明,鸡新城疫病毒对鸡传染性支气管炎病毒增殖无明显促进作用,但无抑制现象;鸡传染性支气管炎病毒浓度过大时,对鸡新城疫病毒有一定抑制作用,在适当浓度时二者之间无干扰作用。以ＮＤＶ１００倍ＩＢＶ１０００倍稀释混合作用后联合接种培养对二者效价均无明显干扰,ＮＤＶ血凝效价可达１１ｌｏｇ２,ＩＢＶ对流免疫电泳沉淀效价可达９ｌｏｇ２,效果最佳。联合培养省时、省力、节约鸡胚损耗。 相似文献
4.
《养殖与饲料．饲料世界》2021,(3)
鸡痘弱毒冻干疫苗,刺种免疫时按瓶签注明的羽份,用灭菌生理盐水稀释,用鸡痘刺种专用针蘸取稀释的疫苗;用鸡痘疫苗专用刺种针浸入疫苗溶液,针槽充满药液后将针轻靠瓶内侧,除去附在针上多余药液。拨开鸡毛、小心刺入鸡翅内侧无血管的翼膜三角区,避免伤及肌肉、关节、血管。一般情况下,疫苗稀释1000只鸡最多需8 mL,也有10 mL。20~30日龄雏鸡刺1针,30日龄以上鸡刺2针(51日龄鸡刺2针),6~20日龄雏鸡用再稀释1倍的疫苗刺1针。后备种鸡可于雏鸡接种后60 d再接种1次,接种后3~4 d,刺种部位微现红肿、结痂,14~21 d痂块脱落。 相似文献
5.
将ＡＥＶ－ＶＲ株接种于６日龄ＳＰＦ鸡胚卵黄囊，孵化至１６日龄时迫杀，采集鸡胚绒毛尿囊膜、尿囊液、胚体称重，研磨、离心取上清液加入福尔马林灭活，以灭菌ＰＢＳ（ｐＨ７．２）稀释成１：１、１：１０、１：２０三种浓度，加入吐温－８０制成水相，另以白油、司斑－８０和硬质酸铝煮沸灭菌制成油相，搅拌混合制成不同浓度的灭活油乳剂疫苗，分别免疫２日龄ＳＰＦ雏鸡，安全可靠．用ＡＥＶ－ＶＲ、ＡＥＶ－１１４３、ＡＥＶ－ＮＨ９３７三株混合毒做攻毒试验证明１：１稀释的灭活苗效果最好，保护率可达１００％．用ＡＥＶ－ＶＲ，ＡＥＶ－１１４３、ＡＥＶ－ＮＨ９３７及以上三株混合毒同法制成１：１浓度的灭活油乳剂疫苗，分别免疫２日龄商品雏鸡后用其相应的毒株做攻毒试验，表明该疫苗是安全的，而且可使免疫鸡抵抗ＡＥＶ的感染，具有良好的免疫效果． 相似文献
6.
用不同毒株鸡传染性支气管炎病毒(F3株、肾型J株、H120-28/86株、H52、H120株)分别按不同浓度(100倍、400倍、700倍和1000倍)与100倍稀释的Lasota鸡新城疫病毒联合接种到10日龄鸡胚尿囊腔，同时设单独培养作对照，96h后收取尿囊液，用对流免疫电泳法检测鸡传染性支气管炎病毒效价，用血球凝集试验检测尿囊液鸡新城疫病毒效价。结果表明.以NDV100倍和IBV1000倍稀释混合作用后联合培养对二者病毒效价均无明显影响，NDV血凝效价可达1b11，IBV对流免疫电泳沉淀效价可达1b11，效果最佳。然后将上述培养液，用福尔马林溶液对其进行灭活，通过常规途径制备鸡新支二联油乳刑灭活苗。将此苗接种于21日龄雏鸡，接种后0，7，14，21，28，35，42，49d分别检测雏鸡NDV抗体和IBV抗体，检出的抗体平均值分别为2，3.6，4.8，5.8，6.2，6.5，6.8，6.5和2，3.3，4.6，5.9，6、2，6.4，6.6，6.4；攻毒保护率为100％。实验证明。以NDV100倍与IBV1000倍稀释混合作用后联合培养的尿囊液制得的鸡新支二联油乳荆灭活苗可以预防鸡新城疫和鸡传染性支气管炎。 相似文献
7.
将鸡传染性喉气管炎(ILT)病毒悬液和鸡传染性支气管炎(IB)病毒悬液，采取不同浓度，不同时间在同一个鸡胚上进行接种，可使两种病毒之间互不干扰，并能在同一鸡胚上共同增殖。取鸡胚绒毛尿囊膜检测传染性喉气管炎病毒(ILTV)，其EID50为10^55/0.2ml，取尿囊液检测传染性支气管炎病毒(IBV)，其EID50为10^63/0．2ml。与单项接种对照组相同；用同胚增殖的绒毛尿囊膜和尿囊液按一定比例混合，制造二联油乳剂灭活苗，免疫后30d和6个月后分别用ILT、IB强毒进行攻毒试验，结果免疫后30d，ILT和IB的保护率均为100％，免疫后6个月，ILT和IB的保护率分别为95.3％、86．7％。本试验结果表明，用此方法增殖病毒，既降低了成本，又保证了免疫效果，为今后制造二联苗提供了科学的依据。 相似文献
8.
采用新城疫病毒克隆７９株和传染性法氏囊病病毒Ｆ１６及Ｖｉｌｅ株为种毒，分别接种ＳＰＦ鸡胚，收取尿囊液毒及胚毒，经甲醛灭活，按一定比例混合，以白油为佐剂制成二联油剂灭活苗。用该苗接种易感鸡，免疫后１４天产生免疫力，２１天攻毒保护率达１００％。１９９３－１９９５年在我省烟台，威海、青岛的一些鸡场免疫２０余万只鸡，获得满意效果。 相似文献
9.
将四株青岛地区分离的肾型传染性支气管炎病毒（Ｈ，ＳＨ，Ｊ和Ｍ株）和传统的疫苗株Ｈ５２株，分别接种于鸡胚，收获尿囊液，经甲醛灭活，按一定比例配合（每头份疫苗中含各株病毒的量不低于１０＾６个气管环组织半数致死量）以矿物油为佐剂制成多价油乳剂灭活苗，本苗接种于１５日龄的雏鸡，免疫后３０天保护率达到１００％，而Ｈ１２０免疫后的鸡群仅获得８０％的保护率，免疫后１６天抗体达到最高水平，免疫后１９０天，抗体效价 相似文献
10.
11.
An investigation was carried out in guinea fowl to determine their susceptibility to infection by Rous sarcoma viruses of subgroups A and C. A standard dose of each subgroup virus was inoculated into 14-day-old embryos via the chorioallantoic membrane (CAM). On the 10th day after inoculation, 50% of the embryonic chorioallantoic membranes were harvested to assess their infection status (CAM(+) or (–)), while the rest were allowed to hatch. The hatchabilities of the embryos inoculated with subgroups A and C were about 50% and 57%, respectively. The relative sensitivities of guinea fowl to infection by viruses of subgroups A and C were observed to be 0.220 and 0.003, respectively, as compared to chickens (1.00). Mortality due to subgroup A virus-induced liver tumours (LT) was 54% and four phenotypic subclasses, namely CAM(+) LT(+), CAM(+) LT(–), CAM(–) LT(+) and CAM(–) LT(–), were observed in guinea fowl as in chickens. However, a higher incidence (31%) of conversely associated phenotypes, i.e. CAM(+) LT(–) and CAM(–) LT(+), were observed in guinea fowl. Mortality caused by subgroup A virus-induced liver tumours was first observed in inoculated guinea fowl keets during the 3rd week after hatching, and 93% of the mortality occurred within 6 weeks. The peak mortality occurred in the 4th week after hatching. The target organs for transformation were considered to be the liver and spleen because of the equal incidence of tumours in these organs. Males and females were equally likely to die from liver tumours. There was also a considerable reduction in the hatchability of guinea fowl embryos from eggs inoculated with either viral subgroup, as reported in chickens.Abbreviations BS
Bryan standard
- CAM
chorioallantoic membrane
- LL
lymphoid leukosis
- LT
liver tumour
- PCV
pock count range
- RSV
Rous sarcoma virus
- tva
tumour virus (subgroup A)
- tvc
tumour virus (subgroup C) 相似文献
12.
Okuda Y Ono M Shibata I Sato S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(12):1561-1566
The pathogenicity of serotype 8 fowl adenovirus (FAV), isolated from gizzard erosions of slaughtered broiler chickens, was investigated. In experiment 1, 29 5-day-old specific-pathogen-free (SPF) chickens were inoculated with the isolates of serotype 8 FAV, M013 (group 1) or G0054 (group 2) strain, via an oral route. There were no clinical signs in any of chickens after inoculation, and mild gizzard erosions were observed macroscopically and microscopically in three inoculated chickens of group 2. FAV was recovered from gizzards and rectums but was not recovered from pancreas and livers from chickens in both inoculated groups. In experiment 2, 27 1-day-old SPF chickens were inoculated with the G0054 strain by intramuscular route. Five, 6, and 3 inoculated chickens died on days 3, 4, and 5 postinoculation (PI), respectively. Four, 3, 1, and 1 inoculated chickens became moribund with severe clinical signs such as ruffled feathers, severe depression and closed eyes from days 3 to 6 PI, respectively. Macroscopically, the common characteristic of the gross lesions of dead chickens and euthanized moribund chickens was discoloration of liver. FAV was recovered from the gizzard, liver, pancreas and rectum. Virus titers in the liver and pancreas were high until day 6 PI. Histologically, necrotizing hepatitis and pancreatitis with intranuclear inclusion bodies were observed in the inoculated chickens. These results indicate that some strains of serotype 8 FAV are able to reproduce not only gizzard erosion by oral inoculation but inclusion body hepatitis (IBH) by intramuscular inoculation. 相似文献
13.
为评价共表达鸡IL-6和H5亚型禽流感病毒HA基因重组鸡痘病毒(rFPV-AIH5AIL6)的免疫抗体消长规律及免疫效力,将重组病毒通过颈部皮下注射和翅部皮下注射2种不同的免疫途径来免疫鸡群,结果发现,2种免疫途径中,重组鸡痘病毒对鸡体质量增加均无影响,而野生型鸡痘病毒均可抑制鸡体质量增加。翅部皮下注射疫苗组能够产生较高的血凝抑制(HI)抗体水平,免疫21d后抗体水平达到高峰,28d后开始下降,49d时仍保持在一定的水平。免疫SPF鸡21d后攻毒,表明该重组病毒能使经滴鼻攻毒的SPF鸡抵抗H5亚型AIV的致死性攻击,保护率为95%,与油苗组相同,与单表达H5亚型禽流感病毒HA基因重组鸡痘病毒组(40%)差异显著;攻毒后3、5、7d采集喉头、泄殖腔棉拭子检测排毒情况,结果发现第3天排毒率最高,其中rFPV-AIH5AIL6免疫组排毒率为最低,显示IL-6在rFPV-AIH5IL6免疫过程中起到了免疫佐剂的作用,这为研制新型的禽流感重组鸡痘病毒疫苗奠定了基础。 相似文献
14.
Genetic susceptibility of indigenous chicks to subgroup A Rous sarcoma virus inoculated via the chorioallantoic membrane. 总被引：3，自引：0，他引：3
An investigation was made using chicks of two Indian indigenous breeds of fowl, Kadaknath and Aseel, to ascertain genetic resistance to infection by Rous sarcoma virus of subgroup A. A standard inoculation dose of 0.2 ml virus containing 1000 pock forming units ml-1 was injected via the chorioallantoic membrane (CAM) into the 11-day-old embryos that were subsequently hatched. The sensitivity of the two indigenous breeds was compared with the highly susceptible exotic White Leghorn (WL) strain maintained in the laboratory. The Kadaknath breed was about three-fold and Assel, about six-fold less sensitive than the WL strain, indicating superiority of the indigenous breeds over the exotic breed of fowl. Most of the CAM-susceptible chicks died of liver tumour (LT) and most of the CAM-resistant chicks survived. However, conversely associated tumour phenotype subclass chicks, i.e. CAM-susceptible LT-negative chicks that survived and CAM-resistant LT-positive chicks that died, occurred consistently in the three breeds of fowl. Nevertheless, the overall survival potential of Kadaknath chicks measured up to 8 weeks post-hatching was greater than that of Aseel chicks. Neither transformation of embryonic tissue prior to hatching nor the visceral metastasis including liver conformed with the degree of CAM-infection as measured by number of pocks on CAMs. 相似文献
15.
从内蒙古牛场疑似山羊痘病毒感染的病羊中分离到1株病毒,命名为NM株。取病羊的皮肤痘疹和水疱作病料样品,分别接种BHK-21传代细胞和10日龄SPF鸡胚。结果表明,BHK-21细胞出现了明显的、规律的细胞病变,鸡胚接种部位出现了明显的痘斑;NM株细胞毒和胚毒均能够被山羊痘病毒阳性血清所抑制。利用1对山羊痘病毒特异性检测引物对NM株病毒进行PCR扩增,获得445 bp片段,与预期大小一致。将扩增产物提纯后克隆至pMD18-T载体,经酶切鉴定正确后,对阳性克隆株进行序列测定,结果表明,NM株的基因片段与已发表的山羊痘病毒毒株的相应基因片段具有高度同源性,同源性在99.2%~100%之间。 相似文献
16.
Seven groups of chickens were challenged with a field isolate of fowl pox virus at 18 weeks old. The birds in the groups that had been vaccinated 3 weeks previously with fowl pox vaccinates showed no signs of disease. Birds which had not been vaccinated against fowl pox developed upper respiratory disease after challenge, and some birds had diphtheritic tracheitis and laryngitis which appeared identical to that commonly seen under field conditions. Seven days after challenge, fowl pox virus was recovered from the tracheas of unvaccinated birds, but not from the vaccinated ones. Intercurrent Mycoplasma gallisepticum infection appeared to extend slightly the period of respiratory disease but was not essential for development of the diphtheritic lesion. 相似文献
17.
Specific-pathogen-free chickens were infected via the trachea when 4 weeks old with 2000 plaque-forming units (PFU) of the virulent Australian infectious laryngotracheitis (ILT) virus strain CSW-1. Titers of ILT virus in the trachea were greatest (10(7.0) PFU/ml in washings, 10(6.0) PFU/g of tissue) 2-4 days postinfection (PI). Infectivity then declined rapidly, to become undetectable by 7 days PI, although highly localized areas of ILT antigen in the tracheal epithelium were occasionally observed by fluorescent antibody staining at 7 and 8 days PI. Tracheal organ cultures established 7 and 8 days PI provided no evidence of latent ILT virus infection at this immediate post-acute stage of pathogenesis. ILT virus was not isolated from peripheral blood leukocytes or lymphoid organs (spleen, bursa, thymus). ILT virus was found in the trigeminal ganglia and/or brain in 14 of 36 chickens (40%) examined between 4 and 7 days after intratracheal inoculation, but it was not in these tissues in five chickens examined at 8 days PI. Virus was also detected at 6 days PI in the trigeminal ganglia in one of five chickens infected by the conjunctival route. These data indicate that the early pathogenesis of ILT (CSW-1) infection frequently involves the tissues of the nervous system. In acute ILT in 4-week-old chickens, interferon-alpha/beta activity was not detectable in serum or tracheal exudates within 14 days PI, but tracheal washings contained significant virus-neutralizing activity by 7 and 8 days PI. In 3-day-old chickens infected via the trachea with 200 PFU of ILT CSW-1, the clearance of ILT virus from the trachea was similar to that observed in 4-week-old chickens, but ILT virus spread systemically to the livers of 20% by 5-7 days PI. 相似文献
18.
S S Saini S S Sodhi N K Maiti S N Sharma 《Comparative immunology, microbiology and infectious diseases》1990,13(1):1-6
The humoral immune response and immunity conferred in chicks were compared following separate and combined oral vaccination with F strain of Newcastle disease virus (NDV) and HP1 strain of fowl pox virus. The haemagglutination inhibition (HI) antibody titre against NDV and passive haemagglutination (PHA) antibody titre against fowl pox virus were comparable in two respective groups. The serum IgG concentration increased significantly after the second vaccination in all the groups. The NDV vaccine induced significantly higher IgG production as compared to fowl pox virus vaccine. There was no significant difference in serum IgG concentration produced by combined vaccine and separate F strain vaccine. The protection afforded by combined and separate vaccinations did not vary significantly against challenge with virulent strains of NDV and fowl pox virus at different stages. 相似文献
19.
传染性喉气管炎新城疫鸡痘重组病毒免疫效力的研究 总被引：1，自引：2，他引：1
在表达鸡传染性喉气管炎病毒(ILTV)糖蛋白gB基因和新城疫病毒(NDV)F基因的重组鸡痘病毒(rF-PV-gB-F)安全性检验合格后,以5.0×101~5.0×104PFU不同含量按0.1mL/鸡的剂量免疫100只30日龄SPF鸡,30d后分组分别用ILTVWG株和NDVF48E9株强毒进行攻击。免疫鸡抗鸡痘病毒抗体都转为阳性,痘反应和接种剂量有关,重组疫苗的最小反应剂量为50PFU。重组疫苗可以诱发对新城疫和传染性喉气管炎的保护,0.1mL/鸡的接种量在500~5000PFU浓度范围内的免疫效果最好,对于ILTV攻击的发病保护率在70%以上,对NDV强毒攻击的抗死亡保护率可以达到80%,这为进一步考察疫苗的免疫效力试验以及进行田间试验奠定了基础。 相似文献
20.
Vaccines against mildly pathogenic avian influenza (AI) have been used in turkeys within the United States as part of a comprehensive control strategy. Recently, AI vaccines have been used in control programs against highly pathogenic (HP) AI of chickens in Pakistan and Mexico. A recombinant fowl pox-AI hemagglutinin subtype (H) 5 gene insert vaccine has been shown to protect specific-pathogen-free chickens from HP H5 AI virus (AIV) challenge and has been licensed by the USDA for emergency use. The ability of the recombinant fowl pox vaccine to protect chickens preimmunized against fowl pox is unknown. In the current study, broiler breeders (BB) and white leghorn (WL) pullets vaccinated with a control fowl poxvirus vaccine (FP-C) and/or a recombinant fowl poxvirus vaccine containing an H5 hemagglutinin gene insert (FP-HA) were challenged with a HP H5N2 AIV isolated from chickens in Mexico. When used alone, the FP-HA vaccine protected BB and WL chickens from lethal challenge, but when given as a secondary vaccine after a primary FP-C immunization, protection against a HP AIV challenge was inconsistent. Both vaccines protected against virulent fowl pox challenge. This lack of consistent protection against HPAI may limit use to chickens without previous fowl pox vaccinations. In addition, prior exposure to field fowl poxvirus could be expected to limit protection induced by this vaccine. 相似文献