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1.
测定了不同栽培温度对虎奶菇菌核蛋白质营养价值的影响.试验结果表明,23~28℃栽培的虎奶菇菌核,其蛋白质的必需氨基酸指数、生物价、营养指数和氨基酸比值系数分别为79.49、74.94、10.11和11.0,均高于自然温度栽培处理,氨基酸评分和化学评分分别为13.36和11.77,均低于自然温度栽培处理.结果表明,23~28℃栽培的虎奶菇菌核蛋白质营养价值高,在大面积生产上可以采用.  相似文献   

2.
虎奶菇覆土栽培技术   总被引:1,自引:1,他引:0  
黄水珍 《食用菌》2004,26(4):34-34
虎奶菇Pleurotus tuber-regium,又名核耳菇、菌核侧耳,是一种珍稀食用菌兼药用菌。主要分布于热带和亚热带地区。由于虎奶菇人工栽培周期长,产量低,成本高,为了开发利用虎奶菇资源,我们于2000年从福建省食用菌菌种站引进了虎奶菇菌种,进行了覆土栽培试验,现将结果报告如下:  相似文献   

3.
虎奶菇生物学特性研究   总被引:4,自引:3,他引:1  
研究了影响好氧菌虎奶菇菌核生长发育的因子。结果表明,虎奶菇菌丝生长最适温度为32℃,菌核生长适温为23~28℃;增养料消毒前较适宜pH为7.5~8;适宜料水比为1∶2.2;较适宜碳源为玉米淀粉和淀粉;较适宜氮源为麦麸和牛粪。  相似文献   

4.
以虎奶菇[Pleurotus tuber-regium (Fr.) Singer]菌丝体和菌核为材料,利用MSAP技术分析其甲基化水平,并对甲基化差异片段进行回收测序,探究虎奶菇生长与DNA全基因组甲基化的关系.结果 表明,菌丝体和菌核的DNA甲基化率分别为7.94%和9.54%,其中半甲基化率分别为4.50%和4.0...  相似文献   

5.
虎奶菇菌核和子实体脂肪酸组成分析   总被引:11,自引:3,他引:8  
采用气相色谱法分析了虎奶菇的脂肪酸组成。其主要脂肪酸成分软脂酸(C16:0)和亚油酸(C18:2)在菌核和子实体中分别占19.1%、18%和68.4%1、62.1%,其中多不孢和脂肪酸亚油酸占优势,表明虎奶菇菌核和子实体可作为药材开发利用。  相似文献   

6.
包水明  李荣同  陈传红  余志坚 《食用菌》2007,29(2):59-60,55
虎奶菇[Pleurotus tuber—regium(Fr.)Sing],中文名菌核侧耳,又名核侧耳、核耳菇、茯苓侧耳、南洋侧耳(日本)等,隶属担子菌纲(Basidiomycetes),伞菌目(Agaricales),侧耳科(Pleurotaceae),侧耳属(Pleurotus),为木腐生真菌。该菌子实体和菌核均可食用或药用,为一种很好的“药食同源”真菌。虎奶菇野生真菌过去仅在我国云南和南省有分布记载,国外日本、东南亚、澳大利亚、非洲亦有分布的记载。笔者在江西崇仁一阔叶林边缘的腐木桩上采到虎奶菇的子实体,这一发现表明,菌核侧耳分布地域可能比以前文献记载的要广的多。  相似文献   

7.
虎奶菇及其栽培   总被引:12,自引:3,他引:9  
目前我国栽培的大多数食用菌其食用的部分都是子实体。只有茯苓食用的部分是菌核,其子实体不能食用,猪苓食用的部分是子实体,菌核则不能食用。然而,在滇西南(腾冲、章凤一带)尚有一种珍稀的食用菌兼药用菌-虎奶菇(张光亚,1984;应建浙,臧穆,1994)其子实体和菌核均可食用。虎奶菇虽然有很高的营养价值和药用价值,可以作为蛋白质(含16%以上)、钾、钙、镁等元素、多糖等活性物质的重要给源,并可制成预防哮喘病、糖尿病、冠心病的天然的保健药品。但迄今我国尚未开发利用。自1993年我们对虎奶菇的生物学特性和栽培  相似文献   

8.
测定了四种碳源对虎奶菇菌核蛋白质营养价值的影响。结果表明,以玉米淀粉为碳源,虎奶薅菌蛋白质的化学评分,必需氨基酸指数,生物价和营养指数分别为11.63、69.80、64.40和9.98,均居四种参试碳源的第一位;氨基酸评分和氨基酸比值系数分分别为14.55和12.45,均居四种参试碳源的第二位,这些结果证实,以玉米淀粉为碳源,虎奶菇菌核蛋白质营养价值高,值得推广应用。  相似文献   

9.
虎奶菇的化学成分与药理作用研究   总被引:2,自引:0,他引:2  
虎奶菇是热带地区一种珍贵的食用菌和药用菌,其菌核主要含有多种单体化合物、蛋白质、氨基酸、矿物质、多糖、不饱和脂肪酸及人体必需的微量元素等物质,其中起主要药理作用的是氨基酸和次生代谢产物、蛋白质、挥发油成分、脂肪酸、多糖等活性物质以及钾、钙、镁等矿质元素。虎奶菇菌核入药可治疗痢疾、胃病、便秘、发烧、感冒、水肿、胸痛、天花、哮喘、高血压、乳腺炎、糖尿病、冠心病、肿瘤等疾病,也能促进胎儿发育,提高早产儿成活率,还有提高人体免疫功能、抗肿瘤等神奇效用,是一种很好的药用真菌,其子实体作为配菜合理膳用,也具有一定的食疗功效。因此,虎奶菇是一种很有发展前景的新的食用菌,应当利用现代技术手段和方法,以循证医学的观念,加强对虎奶菇的深入研究,充分发挥其营养价值和药用价值。  相似文献   

10.
不同液体发酵培养基对虎奶菇菌丝生长的影响   总被引:1,自引:1,他引:0  
虎奶菇,[Pleurotus tuber-regium (Fr.)Singer.]也称地核侧耳,为腐生性担子菌。分布热带和亚热带地区,主产非洲,我国主要分布在云南省藤冲和章风县一带。在非洲,虎奶菇是尼日利亚诸多地区的主要经济来源,不仅在于其菌核和子实体町以食用,重要的是菌核长期以来被民间用于治疗多种疾病,如治疗乳腺炎、便秘、胃痛、天花、糖尿病等症,价格非常昂贵。  相似文献   

11.
Zusammenfassung Die Leistungsprüfungen wurden im Zeitraum 1997 bis 2003 mit den Unterlagen Gisela 4 und 5, den Klonnummern 195/20 und 497/8 aus der Gisela-Serie sowie Weiroot 10, 13, 53, 72 und 158 durchgeführt. Dabei dienten Sämlinge von P1 (bulgarische Selektion aus Prunus mahaleb) als Kontrolle. Alle Unterlagen waren mit der Sorte Stella veredelt und im Dezember 1996 in der Versuchsanlage der Agraruniversität in Plovdiv, Bulgarien, im Abstand von 6 m×4,5 m gepflanzt worden. Dabei erfolgte ein Pflanzschnitt. Nach Abschluss der natürlichen Kronenentwicklung wurde jedes Jahr ein Winterschnitt vorgenommen. Der Boden wurde durch mechanische Bearbeitung offen gehalten und nach dem 4. Standjahr wurden die Baumstreifen mit Herbiziden behandelt. Die Wasserversorgung erfolgte durch eine dem natürlichen Gefälle folgende Überflutung, allerdings nicht immer zum optimalen Zeitpunkt, da keine eigene Wasserquelle zur Verfügung stand.Basierend auf den Ergebnissen bis zum Anfang des 7. Standjahres können die untersuchten Unterlagen in zwei Gruppen differenziert werden: starkwüchsig—Weiroot 10, P1 und Weiroot 13; mittelstarkwachsend bis schwachwüchsig—Gi 497/8, Gisela 4, Weiroot 53, Weiroot 158, Gi 195/20, Weiroot 72 und Gisela 5. Letztere zeichnete sich durch besondere Schwachwüchsigkeit aus. Die meisten Wurzelschosser bildeten Gisela 4, Weiroot 10 und Weiroot 13. Weiroot 53, Weiroot 72 und Weiroot 158 entwickelten deutlich weniger und P1, Gisela 5, Gi 195/20 sowie Gi 497/8 keine Wurzelschosser. Den frühesten Blühbeginn induzierte Gisela 4. Die anderen Unterlagen führten, in Abhängigkeit von den Temperaturbedingungen des jeweiligen Jahres, zu einer Verspätung der Blüte: P1 und Weiroot 10 um 1–2 Tage; Gi 497/8, Weiroot 13 und Weiroot 158 um 2–4 Tage; Weiroot 72 um 2–7 Tage; Gi 195/20 um 3–6 Tage; Weiroot 53 um 3–8 Tage und Gisela 5 um 3–10 Tage. Die Reifezeit der Früchte war bei den Bäumen auf Gisela 5 im Vergleich zu den anderen Varianten um 2–3 Tage verspätet. Gisela 5, Weiroot 72 und Gisela 4 induzierten bei der aufveredelten Sorte die höchsten Ertragsleistungen, P1 die geringsten. Bei den Bäumen auf Gisela 5 war die Fruchtgröße geringer als bei den anderen Unterlagen. Bäume auf Gisela 5 brauchen intensive Pflege. Nur wenn alle Produktionsfaktoren und kulturtechnischen Maßnahmen optimiert werden, kann das hohe Ertragspotenzial dieser Unterlage ausgeschöpft werden.  相似文献   

12.
AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation.  相似文献   

13.
AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P<0.01). The ET content in plasma also decreased significantly by L-Arg(P<0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration.  相似文献   

14.
AIM:To investigate the effect of puerarin on pulmonary vessel collagen metabolism in pulmonary hypertension rats induced by chronic hypoxia and hypercapnia.METHODS:Collagen Ⅰ, Ⅲ and their mRNA were observed in pulmonary arterioles by the technique of immunohistochemistry and in situ hybridization.RESULTS:① Light microscopy showed media thickness of pulmonary arterioles was much higher in HH(hypoxic-hypercapnia) group than that of NC(normal control) group, and, vessel cavity turned more straiter in HH group than that of NC group.However, the damage of pulmonary arterioles in HP(hypoxic-pueratin) group was much slighter than that of HH group. ② The levels of plasma ET-1 and lung homogenates Hyr were much higher in HH group than those of NC group(P<0.01), and lower in HP group than HH groups(P<0.01).Plasma NO content in group HH was lower than that of group NC(P<0.01), it was higher in group HP than that of group HH(P<0.01).③Expression of collagen Ⅰ and collagen Ⅰ mRNA in pulmonary arterioles were significantly higher in HH groups than those of NC group (P<0.01), and they were lower in HP group than those of HH group (P<0.01).Expression of collagen Ⅲ and collagen Ⅲ mRNA showed no difference among three groups(P>0.05).CONCLUSION:Puerarin inhibited the deposition of collagen and improved pulmonary vessel remodeling.  相似文献   

15.
AIM: To examine the autoantibody against α1-adrenoceptor and its biologic activities during the development of renal hypertension. METHODS: Renal hypertension of rat was achieved by clipped renal artery, the titre of autoantibody to α1-adrenoceptor was detected using ELISA immunoassay. Furthermore, the biological offects of these autoantibodies on cultured cardiomyocytes were also examined. RESULTS: After two weeks of clipping renal arteries, both the frequency of occurrence and the titre of autoantibodies to cardiac α1-adrenergic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 12 weeks. The biological effects of these autoantibodies displayed an "agonistic-like" activities on the beating frequency of cultured neonatal cardiomyocytes. CONCLUSION: Autoantibodies against α1-adrenoceptor may play a role in the elevation of peripheral vascular resistance and in the development of cardiac hypertrophy in rats with renal hypertension.  相似文献   

16.
Abstract

Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared.  相似文献   

17.
Abstract

This review is based partly on complete articles and partly on abstracts. Three of the 60 articles deal with the total uptake of elements in strawberry plant organs in two different strawberry production systems, both considered as optimal concerning amount and balance of elements. The effect on fruit quality may be dramatic if the level of a particular element is outside this range, but there may also be effects initiated by differences within the optimal range of elements. Most articles refer to product oriented quality, but some focus on consumer oriented quality, as discussed by Shewfelt (1999). The discussion here is on a general basis, so one should keep in mind that there are cultivar differences and that specification of nutrition ideally should mirror the needs of a single cultivar, or a group of cultivars with similar requirements. Also, to get a complete understanding of the subject future reviews should embrace a broader access of information including the effect on plant development of individual elements, such as the role of calcium in fruit firmness and its importance in cell wall structure. However, the intention here is to narrow the information to results that suggest a direct connection between nutrient uptake and fruit quality.  相似文献   

18.
The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species.  相似文献   

19.
Habitat for wide-ranging species should be addressed at multiple scales to fully understand factors that limit populations. The marbled murrelet (Brachyramphus marmoratus), a threatened seabird, forages on the ocean and nests inland in large trees. We developed statistical relationships between murrelet use (occupancy and abundance) and habitat variables quantified across many spatial scales (statewide to local) and two time periods in California and southern Oregon, USA. We also addressed (1) if old-growth forest fragmentation was negatively associated with murrelet use, and (2) if some nesting areas are more important than others due to their proximity to high quality marine habitat. Most landscapes used for nesting were restricted to low elevation areas with frequent fog. Birds were most abundant in unfragmented old-growth forests located within a matrix of mature second-growth forest. Murrelets were less likely to occupy old-growth habitat if it was isolated (> 5 km) from other nesting murrelets. We found a time lag in response to fragmentation, where at least a few years were required before birds abandoned fragmented forests. Compared to landscapes with little tono murrelet use, landscapes with many murrelets were closer to the ocean's bays, river mouths, sandy shores, submarine canyons, and marine waters with consistently high primary productivity. Within local landscapes (≤ 800ha), inland factors limited bird abundance, but at the broadest landscape scale studied (3200 ha), proximity to marine habitat was most limiting. Management should focus on protecting or creating large, contiguous old-growth forest stands, especially in low-elevation areas near productive marine habitat. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

20.
AIM: To detect the role of cyclic nucleotides in the alleviation of hypoxic pulmonary vasoconstriction (HPV) in chronic hypoxic animals. METHODS: The intracellular cAMP and cGMP of the cultured porcine pulmonary arterial smooth muscle cells (PASMC) and endothelial cells (PAEC) were assayed by RIA. The length of single PASMC during acute hypoxia was measured by imaging analysis system. RESULTS: The basal levels of cAMP and cGMP in PASMC and cGMP in PAEC of Chronic hypoxic groups decreased remarkably compared with normoxic groups (P<0.01). Under acute hypoxia, the contents of cAMP and cGMP in PASMC of chronic hypoxic groups increased significantly (P<0.01). Meanwhile, the percentage of PASMC with weak constrictive response in chronic hypoxic group was higher than that of control group. CONCLUSION:It's suggested that the changes of cAMP and cGMP in chronic hypoxic PASMC and PAEC might contribute to the increase in the basic tension of pulmonary artery and the alleviation of HPV in chronic hypoxic animals.  相似文献   

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