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1.
为了解目前我国鸽群中鸽圆环病毒(pigeon circovrius,PiCV)的流行现状及遗传变异情况,2021—2022年对山东等11个省(自治区)PiCV感染状况进行了调查,共采集鸽相关样品658份,采用PCR方法进行PiCV病原学检测,并对3份PiCV病原学阳性样品进行基因组序列测定及遗传进化分析。结果显示:在主动监测的9个省(自治区)49个采样点中,检出464份PiCV病原学阳性样品,个体阳性率为75.20%(464/617),场点阳性率为91.84%(45/49),其中河南省个体阳性率最高(100%);在被动监测的3个省41份病死鸽组织样品中,共检出28份PiCV病原学阳性样品;3份病原学阳性代表样品(QingDao 957、JiLin2、FuJian 1179)与已报道的24条PiCV全基因组序列同源性为87.30%～95.90%;QingDao 957和JiLin 2与国内报道的JS15-1序列亲缘关系较近,而FuJian 1179与国外报道的PL53参考序列属于同一分支,推测QingDao 957和JiLin2毒株在国内流行的PiCV中占主导地位,而FuJian 117... 相似文献
2.
为了解云南省玉溪市活禽交易市场禽流感病毒污染情况,2021年对该市8个县（市、区）活禽市场进行采样检测。全年共采集63场次1 455份棉拭子样品（禽咽喉/泄殖腔双拭子860份、环境拭子317份、运输工具拭子278份）,采用荧光RT-PCR方法进行流感病毒A型、H5/H7/H9亚型流感病毒核酸检测。共检出A型流感病毒核酸阳性435份,未检测到H5和H7亚型病毒核酸阳性;除检出7份未分型阳性样品外,在44场次检出H9亚型病毒核酸阳性428份,场次阳性率为69.84%,样品阳性率为29.42%,其中活禽、环境、运输工具样品阳性率分别为32.56%、25.87%、23.74%,差异具有统计学意义（P＜0.05）。一年四季均能检测到H9亚型病毒阳性,但3—6月份样品阳性率低于年均阳性率,1、2、7、10、12月样品阳性率明显高于其他月份,各月份间样品阳性率差异具有统计学意义（P＜0.01）。各县（市、区）的场次阳性率存在差异（P＜0.05）,但样品阳性率差异无统计学意义（P＞0.05）。结果表明：玉溪市活禽市场H5、H7亚型高致病性禽流感病毒的污染状况得到有效控制,但H9亚型禽流感病毒污染面依然广,污染率较高;不同月份间阳性率差异明显,但季节性差异缩小。结果提示,应继续加强活禽市场H9亚型禽流感病毒污染监测,同时强化活禽市场的检疫监管,严格执行消毒和休市制度,防止活禽市场禽流感病毒传入养殖环节。 相似文献
3.
为了解冬季新疆乌鲁木齐市活禽市场交易禽类及市场外环境中禽流感病毒污染情况及其流行规律,对某交易市场内的鸡、鸭、鹅、鸽等活禽,采集85份活禽全血样本和143份棉拭子样本(103份活禽泄殖腔/咽喉棉拭子、40份环境棉拭子),分别采用血清学和实时荧光定量(rRT-PCR)方法,检测禽流感免疫抗体和禽流感病毒核酸,并对核酸阳性样品进行病毒亚型确定。结果显示:85份全血样本中,H5(Re-11)株免疫抗体合格32份,合格率为37.6%;H5(Re-12)株免疫抗体合格34份,合格率为40%;H7(Re-2)株免疫抗体合格46份,合格率为54.2%。143份棉拭子样本中,禽流感病毒核酸阳性36份,阳性率为25.17%。其中:活禽棉拭子阳性29份,阳性率为28.15%;交易区外环境棉拭子阳性7份,阳性率为17.5%。36份禽流感病毒核酸阳性样本中,H5亚型3份,阳性率为8.33%;H9亚型22份,阳性率为61.11%;未定型11份,阳性率为30.56%。结果表明:该市场交易活禽流感病毒感染率较高,且强制免疫的H5、H7亚型禽流感免疫抗体合格率较低,存在疫情发生和散播风险。结果提示,应加强活禽交易市场的消毒与管理,降低禽流感病毒通过活禽交易市场传播的风险。 相似文献
4.
为了解新疆乌鲁木齐市活禽交易市场内禽流感病毒污染状况以及亚型分布情况,掌握活禽档口、活禽屠宰点及活禽运输车辆的禽流感病毒流行规律,对采集的437份棉拭子样本,采用实时荧光定量RT-PCR方法检测禽流感病毒核酸,再对阳性样品进行核酸分型。结果显示:473份样品中,检出禽流感病毒核酸阳性115份,阳性检出率为24.31%,其中H5亚型26份,阳性检出率为22.6%,H9亚型45份,阳性检出率为39.13%,未定型44份,阳性检出率为38.26%,未检出H7亚型;活禽运输车、活禽档口和活禽屠宰点的阳性检出率分别为29.69%、24.15%和21.74%;活禽档口中的污水(44.90%)、笼具(34.69%)、粪便(24.49%),活禽屠宰点的脱毛机(47.80%)、接血槽(34.78%),以及活禽运输车的装载笼具(56.25%)的阳性检出率较高。结果表明:该活禽交易市场禽流感病毒污染状况较为严重,污染范围较广,活禽运输车、活禽档口和活禽屠宰点均有污染;活禽档口中的污水、笼具、粪便,活禽屠宰点的脱毛机、接血槽,以及活禽运输车的装载笼具为禽流感病毒散播的关键风险点。结果提示,需加强市场日常管理及清洗消毒,降低禽源流感病毒通过活禽交易市场传播的风险。本调查为制定科学有效的活禽交易市场日常管理及消毒措施提供了技术支持。 相似文献
5.
为了解安徽省A型禽流感病毒感染情况,2018年4—5月,在安徽省北部、中部和南部7个市,随机选取各类型场点173个,在每个场点内随机采集35份咽喉/泄殖腔和环境拭子样品,使用荧光RT-PCR进行A型、H5亚型、H7亚型流感病毒核酸检测。结果显示：在4个养殖场、3个活禽批发市场和17个农贸市场检出了A型禽流感病毒,场点阳性率分别为2.9%（4/140）、75.0%（3/4）、80.9%（17/21）,未检出H5和H7亚型流感病毒核酸;A型禽流感群体流行率为11.2%（95%CI：6.5%~15.8%）,个体流行率为1.2%（95%CI：0.9%~1.5%）;活禽交易市场内,禽群感染A型禽流感病毒的风险较高（OR=136,95%CI：32.7~549.4）。结果表明,A型禽流感病毒在养殖和交易环节存在一定的污染,而在活禽交易市场的污染更严重,是今后禽流感防控的重点,需继续加强活禽交易市场的管理和消毒灭源工作。 相似文献
6.
《畜牧与兽医》2015,(10):13-17
为了解目前我国活禽市场H9N2亚型禽流感病毒(avian influenza virus,AIV)流行和增殖情况,2014年在上海、江苏、安徽和广东随机选取58个活禽市场,采集12 635份活禽拭子样品进行病毒分离、RT-PCR和序列测定分析。结果:15.11%拭子样品和98.28%的活禽市场是H9N2亚型AIV阳性。鸡、鸭、鹅、鸽样品阳性率分别为22.98%(1730/7529)、3.62%(94/2594)、4.08%(67/1643)、2.09%(18/861);上海、江苏、安徽和广东的样品阳性率分别为24.11%(688/2853)、8.43%(284/3368)、15.27%(475/3110)、13.95%(461/3304)。活禽零售市场阳性率(34.40%)显著高于活禽批发市场感染率(12.24%)。随着家禽在批发市场内停留时间的延长,H9N2亚型AIV阳性率从11.45%(停留8 h内)上升到29.13%(停留48 h以上)。遗传进化分析表明:所检出的H9N2亚型AIV均属于2007年以来我国H9亚型AIV主要流行分支,即h9.4.2.5分支。这些流行病学调查结果提示我国长三角和珠三角活禽市场的家禽(尤其是鸡)H9N2亚型AIV感染率很高,并且随着活禽在批发市场内停留时间延长,以及向零售市场转移,H9N2亚型AIV存在一个显著增殖和扩散的过程。 相似文献
7.
为了解湖南省永州市活禽市场禽流感病毒污染状况,2017年4—12月,永州市动物疫病预防控制中心在冷水滩区采集571份家禽和环境样品,进行了H5、H7亚型禽流感病毒核酸实时荧光定量RT-PCR 检测。结果显示：571份样品中,检出H5亚型禽流感病毒核酸阳性样品44份,阳性检出率为7.7%,未检出H7亚型禽流感病毒阳性样品;除5月份外,每月都能监测到阳性样品,且冬季阳性检出率明显偏高;活禽在摊位中停留时间越长,禽流感病毒阳性率越高;鸭源样品和污水样品中的禽流感病毒检出率最高,城乡农贸市场的病毒阳性检出率高于城区活禽交易市场。调查结果表明,活禽市场中的H5亚型禽流感病毒污染率较高,尤其是水禽和污水以及卫生条件较差的市场。因此,要严把市场休市清洗消毒关,提升市场环境卫生水平,严格活禽调运检疫和监管,禁止陆禽和水禽混合销售。 相似文献
8.
为掌握玉溪市禽流感在养殖场和活禽市场流行情况，2022年采集玉溪市9个县(市、区)32个乡(镇)62个村90个养殖场(户)及21个活禽市场2 280份样品进行监测。采用荧光RT-PCR方法对A型禽流感病毒检测并进行H5/H7/H9亚型禽流感病毒核酸分型。2022年全市共检出A型禽流感病毒核酸阳性240份，样品阳性率为10.53%,其中H9亚型233份、不确定型7份，未检出H5/H7亚型。活禽市场A型禽流感病毒核酸阳性率(31.47%)显著高于养殖场阳性率(0.27%),差异具有统计学意义(P<0.01);秋冬季节样品阳性率较高，差异具有统计学意义(P<0.01)。结果：玉溪市禽流感病毒主要分布于活禽市场，且以H9亚型流行为主，污染面较广，不同季度阳性率差异明显，但季节性差异缩小。结果提示：活禽市场是当前禽流感防控的重点，继续加强活禽市场的监测，同时强化活禽市场的检疫监管，严格执行消毒和休市制度，防止活禽市场禽流感病毒进入养殖环节。 相似文献
9.
为了解广东江门地区活禽交易市场禽流感隐性带毒情况,自2011年1月至201 3年12月,每月2次到活禽交易市场采集样品,应用实时荧光RT-PCR方法检测禽流感病毒核酸.结果显示,该地区活禽交易市场A型流感病毒核酸阳性率逐年增高,主要受低致病性禽流感病毒污染.低致病性禽流感隐性带毒现象主要发生在鸽、鹌鹑和鹧鸪等珍禽上. 相似文献
10.
4月10日,农业部发布消息,又从江苏、浙江、安徽的个别活禽交易市场中检出14份H7N9禽流感病毒阳性样品。对此,中国动物疫病预防控制中心主任才学鹏介绍,截至目前,阳性样品均来自活禽交易市场,养殖场中目前没有检出阳性样品。来自正规养殖企业、经过严格检疫的家禽产品可以放心食用。
才学鹏介绍,截至4月10日,已完成了对重点地区3175份样品的检测,其中,包括57个养殖场的样品1635份、19个活禽交易市场的样品1222份、3处环境样品50份和9处野鸟粪便样品366份。从上述样品中检出34份H7N9禽流感病毒阳性样品,均来自4省市的8个活禽市场,涉及上海20份、安徽1份、江苏11份和浙江2份。其中,21份来自鸡、3份鸽子、3份鸭、7份环境。 相似文献
才学鹏介绍,截至4月10日,已完成了对重点地区3175份样品的检测,其中,包括57个养殖场的样品1635份、19个活禽交易市场的样品1222份、3处环境样品50份和9处野鸟粪便样品366份。从上述样品中检出34份H7N9禽流感病毒阳性样品,均来自4省市的8个活禽市场,涉及上海20份、安徽1份、江苏11份和浙江2份。其中,21份来自鸡、3份鸽子、3份鸭、7份环境。 相似文献
11.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献12.
Heartworm in dogs in Canada in 1982 总被引：8，自引：8，他引：0
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1983,24(7):227-229
13.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献14.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
15.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献16.
17.
Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献