共查询到20条相似文献，搜索用时 187 毫秒
1.
1995年夏天，笔者对1批雏番鸭被阵雨淋后暴发鸭疫日氏杆菌疫病进行诊治，现将材料整理如下：1发病情况1995年7月12日下午，某场1批1200羽3周龄的番鸭苗已装笼上车即将发往外地，突然遭受雷雨淋湿，被淋湿的番鸭苗身上直冒热气。因此，暂不发出，把番鸭苗全部放回育雏室。结果当晚发病死亡13羽。以后病情逐渐严重，死亡增多，最多的1天死亡达100多羽。发病初期疑诊为鸭感冒、巴氏杆菌病、大肠杆菌病等。曾用青霉素、安乃近、链霉素、庆大霉素、氰霉素等药物治疗。病情时好时坏，相继在本地区流行2个月，计发病5000多羽，死亡1070羽。2临论症… 相似文献
2.
鸭疫巴氏杆菌病，又称鸭传染性浆膜炎，是由鸭疫巴氏杆菌即鸭疫里默氏杆菌引起鸭的一种急性或慢性接触性传染病。我市从１９９５年在甘棠发现本病以来，其危害性日益突出，已成为影响我市养鸭业的一种主要传染病。为了更好地防治本病，提高广大鸭农饲养效益，现将本市鸭疫巴氏杆菌的诊治情况介绍如下：１流行病学本病主要发生于７～５６日龄的麻鸭、半番鸭和番鸭，其中以２～３周龄的鸭最易感，而１周龄以下和８周龄以上的不易感染发病。本病在我市一年四季均有发生，但以冬春气候变化剧烈时较易感，此外，还与栏舍环境、饲管水平及其它… 相似文献
3.
鸭疫巴氏杆菌病的诊断及治疗陈同海，陈房桂，许保柱，叶文辉（深圳市皇岗动植物检疫局动检二科深圳市５１８０４５）郑星道，包傻珊（吉林农业大学动物科学系长春１３０１１８）鸭疫巴氏杆菌病又称鸭传染性浆膜炎、鸭败血症，是鸭、火鸡和多种禽类的一种接触性传染病，主... 相似文献
4.
5.
１９９５年下半年以来广西部分地区暴发一种主要侵害２－３周龄幼鸭的急性或亚急性传染病，主要症状为眼鼻有分泌物，下痢，共济失调，抽搐，死亡率１５－２０％，病变以纤维素性心包炎，肝周炎和气囊炎为特征。从病死鸭中分离出均匀一致，两极浓染的小杆菌，经实验室检验，确诊为鸭疫巴氏杆菌病。经药敏试验，分离菌对氯霉素和庆大霉素都敏感，试用病死鸭的肝，脾组织，制成组织灭活苗，免疫幼鸭，取得较好的免疫效果。 相似文献
6.
鸭疫巴氏杆菌与大肠杆菌二联苗研究 总被引：12，自引：0，他引：12
用临床分离到的鸭疫巴氏杆菌与大肠杆菌制成二联灭活苗，并加入免疫调节剂左旋咪唑。经实验室与野外扩大试验证实，该疫苗具有确实的预防效果，在免疫１０天后，雏鸭即可产生坚强的免疫力，攻毒保护率达１００％，野外试验可明显降低鸭疫苗杆菌与大肠杆菌感染造成的死亡。 相似文献
7.
鸭疫里氏杆菌病灭活疫苗的研制 总被引：1，自引：1，他引：0
鸭疫里氏杆菌是主要感染于雏鸭的传染病。目前 ,防治本病主要用药物治疗和疫苗预防。现介绍鸭疫里氏杆菌病灭活苗的研制。1 材料与方法1 .1 材料菌种 :本实验室分离保存的菌株ＳＲＡ。试验鸭和鸡胚 :来自健康种鸭场和鸡场。白油 :杭州炼油厂生产。吐温 -80、司本 -80 :上海大众制药厂生产。硬脂酸铝 :上海远航化工厂生产。福尔马林 :上海化学试剂采购供应站。高速搅碎机 :上海标本模型厂。1 .2 方法1 .2 .1 细菌的增殖 :将菌种接种于自行研制的生化或生物增菌培养液 ,3 7℃繁殖 2 4ｈ～ 48ｈ ,收集菌液 ,纯度检查合格方可用于制苗。1 .2… 相似文献
8.
鸭传染性浆膜炎原称鸭疫巴氏杆菌病，是由鸭疫里氏杆菌（原称鸭疫巴氏杆菌）所引起小鸭的一种急性或慢性的接触性传染病。临诊以眼、鼻分泌物增多，共济失调、抽搐或歪头斜颈和纤维素性心包炎、肝周炎、气囊炎等病变为特征。我省于１９８８年首次报道了本病的存在，近年来，随着鸭的几种主要病毒性疾病得到有效预防以来，本病的危害性显得日益突出，并逐渐发展成为影响我省养鸭业发展的一种主要传染病。现将本人在鸭传染性浆膜炎诊治中的一些体会介绍如下：１ 流行病学特点 １１ 易感动物 主要发生于番鸭、半番鸭以及小麻鸭；发病的日… 相似文献
9.
10.
鸭传染性浆膜炎又名鸭疫里氏杆菌病，原名鸭疫巴氏杆菌病，主要侵害2-3周龄雏鸭，能引起雏鸭的大批死亡和发育迟缓，造成很大的经济损失，是当前危害养鸭业的主要传染病之一。 相似文献
11.
Pasteurella anatipestifer (PA) serotypes 1, 2, and 5 grew to high densities in tryptic soy broth and tryptose broth (TB) when the media were continuously shaken or aerated. Growth in 100 ml to 15 liters of TB exceeded an absorbance of 1.0 at a wavelength of 525 nm (about 0.7 for a 1/3 dilution) and contained more than 10(10) colony-forming units per ml. A bacterin was prepared from the three serotypes of PA grown in aerated TB. Two subcutaneous injections of this bacterin protected 70% to 85% of ducklings against experimental challenge with each of the three PA serotypes, which killed 90% to 100% of unimmunized controls. The bacterin could be diluted 1/5 without decreasing protection below 80%. Field studies on Long Island duck farms in 1980 and 1981 demonstrated significant reductions in mortality with the use of the broth-grown PA bacterin. 相似文献
12.
Immunostimulants were given to rainbow trout for assaying effects on modulating non-specific defense mechanisms, specific immune response, and protection levels against pathogen challenge with Aeromonas salmonicida. Three drugs, levamisole (an approved veterinary drug in the USA), a quaternary ammonium compound (QAC), and a short-chain polypeptide (ISK) were found to affect the non-specific defense mechanism activities, which were measured by changes in circulatory neutrophil and phagocytic activity levels, and the specific immune response factors, which were measured by numbers of plaque-forming cells, and circulatory antibody levels. When given alone, the immunostimulants elevated the non-specific factors. When injected in combination with an A. salmonicida O-antigen bacterin, the non-specific factors were further elevated, and the specific response was raised over samples taken from fish given the bacterin without the immunostimulants. Challenge tests with the virulent pathogen, A. salmonicida, showed a 5-6 day delay in the onset of mortalities in the fish given the immunostimulants alone, and a 12-14 day delay when immunostimulants given were combined with the bacterin. In the groups given the QAC or ISK with the bacterin, there was a 20% and 40% survival rate, respectively. 相似文献
13.
One hundred and ninety four avian pathogenic Escherichia.coli (APEC) isolated from different pathotypes of chickens in Hebei province,Shandong province,Henan province,Jiangsu province,Shanxi province and Liaoning province in China between May 2005 and March 2016 were investigated by determination of biochemical characters,O serogroup identification and drug sensitivity test.Out of 194 isolates, 124 strains(63.9%) could be grouped to serogroups O1(3),O2(26),O57(15),O65(47) and O78(33).The drug sensitivity test used eight antibiotics (ampicillin, neomycin, doxycycline, sulfamonomethoxine,tilmicosin,ciprofloxacin ceftiofur and florfenicol) by one strain of O1,O2,O57,O65,O78 serogroup,respectively.The results showed that all strains were sensitive to ciprofloxacin and neomycin, and resistance to other drugs at varying degrees.This study demonstrated that the high prevalence of O65,O78,O2 and O57 isolates existed and provided the basis for the development of multivalent bacterin in future. 相似文献
14.
对2005年5月~2016年3月自河北、山东、河南、江苏、陕西、辽宁等省发病鸡场疑似大肠杆菌病的病死鸡病变部位分离的194株禽致病性大肠杆菌进行了生化特性测定、O抗原血清群鉴定及药敏试验。试验用5种大肠杆菌单因子（O1、O2、O57、O65、O78）阳性血清进行O血清群鉴定,确定了124株O血清群,占鉴定菌株数的63.9%（124/194）,其中O1 3株、O2 26株、O57 15株、O65 47株和O78 33株,O65和O78为优势菌株,而O2和O57血清群菌株次之,O1血清群菌株最少。8种药物（氨苄西林、新霉素、多西环素、磺胺间甲氧嘧啶、替米考星、环丙沙星、头孢噻呋和氟苯尼考）的药敏试验结果表明,受试的5株大肠杆菌分离株均对环丙沙星和新霉素敏感,而对其他药物均有不同程度的耐受。本研究结果表明O65、O78、O2和O57血清群高度流行,为进一步研发多价菌苗奠定了基础。 相似文献
15.
van Overbeke I Chiers K Donne E Ducatelle R Haesebrouck F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):289-293
The efficacy of two bacterins containing an Actinobacillus pleuropneumoniae serotype 10 strain was evaluated. The bacterial cells constituting bacterin 1 and 2 were grown under nicotinamide adenine dinucleotide (NAD)-rich (low-adherence capacity to alveolar epithelial cell cultures) and NAD-restricted (high-adherence capacity to alveolar epithelial cell cultures) conditions, respectively. Ten pigs were vaccinated twice with the bacterin 1 and nine pigs with the bacterin 2. Ten control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 106.5 colony-forming units (CFU) of an A. pleuropneumoniae serotype 10 strain. In the bacterin 1 and 2 group, three and two pigs died after inoculation, respectively. Only two pigs of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The percentage of pigs with severe lung lesions (> 10% of the lung affected) was 100% in the control group, 70% in the bacterin 1 group and 22% in the bacterin 2 group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 7.0 x 10(6) CFU/g in the control group, 6.3 x 10(5) CFU/g in the bacterin 1 group and 1.3 x 10(6) CFU/g in the bacterin 2 group. It was concluded that both bacterins induced partial protection against severe challenge. Furthermore, there are indications that the bacterin 2, containing A. pleuropneumoniae bacteria grown under conditions resulting in high in vitro adhesin, induced better protection than the bacterin 1. 相似文献
16.
Baccaro MR Hirose F Umehara O Gonçalves LC Doto DS Paixão R Shinya LT Moreno AM 《Veterinary journal (London, England : 1997)》2006,172(3):526-531
A field study was conducted in Brazil to evaluate the efficacy of single vaccination of pigs with two bacterins to prevent Mycoplasma hyopneumoniae lung lesions. The first (T1) treatment group (174 pigs) was injected with 2 mL of saline solution; group T2 (177 pigs) with 2 mL of bacterin A, and group T3 (174 pigs) with 2 mL of bacterin B. On days-on-test (DOT) 0, 35, 66, 97 and 125, blood samples and tonsil swabs were collected from selected pigs for antibody determination (indirect ELISA) and PCR assay for the presence of M. hyopneumoniae. Pigs were slaughtered on DOT 126-129 and lung lesions were scored blindly. Bacterin A vaccinated pigs had significantly (P < or = 0.05) lower lung lesion scores (0.2%) than bacterin B (0.4%) or saline-treated pigs (1.2%); there was also a significantly lower (P < or = 0.05) number of pigs with lung lesions (27.1%), than bacterin B (38.2%) or saline-treated (55.4%) pigs. The two vaccines had similar (P>0.05) results in terms of mean weight gain, average daily weight gain, feed efficiency, frequency of PCR positives, and there was similar antibody conversion (ELISA). It was concluded that although the productivity parameters and antibody conversions were similar, bacterin A was more effective in preventing and reducing the severity of lung lesions than bacterin B. 相似文献
17.
Cattle were vaccinated against leptospirosis with 3 bacterin preparations: (a) trivalent (serotypes grippotyphosa, pomona, and hardjo) whole cell bacterin; (b) bivalent pomona and hardjo whole cell bacterin; and (c) pentavalent (canicola, grippotyphosa, icterohaemorrhagiae, pomona, and hardjo) cell wall bacterin. Microscopic agglutinating antibody responses in cattle given the last-named bacterin were higher than those in cattle vaccinated with the 2 whole cell bacterins (trivalent and bivalent). However, microscopic agglutinating antibody responses occurred in all vaccinated cattle after they were given a challenge inoculation of serotype hardjo. Leptospires were isolated from 2 of 4 challenge controls (i.e., not given any bacterin), but none of the 15 vaccinated cattle given any one of the bacterins and then challenge inoculated with hardjo became culturally positive. It was concluded that the 3 multivalent bacterins were protective against experimental challenge inoculation of hardjo. 相似文献
18.
鸭巴氏杆菌病灭活疫苗的研制与应用初报 总被引：1，自引：0，他引：1
鸭巴氏杆菌病发病急、死亡快，用禽霍乱疫苗免疫，效果欠佳，为此，我们从发病鸭中分离一株鸭巴氏杆菌强毒株，制成氢氧化铝甲醛灭活疫苗，试用１００多万羽份，表明对该病具有良好的保护作用。 相似文献
19.
I. van Overbeke K. Chiers E. Donne R. Ducatelle F. Haesebrouck 《Zoonoses and public health》2003,50(6):289-293
The efficacy of two bacterins containing an Actinobacillus pleuropneumoniae serotype 10 strain was evaluated. The bacterial cells constituting bacterin 1 and 2 were grown under nicotinamide adenine dinucleotide (NAD)‐rich (low‐adherence capacity to alveolar epithelial cell cultures) and NAD‐restricted (high‐adherence capacity to alveolar epithelial cell cultures) conditions, respectively. Ten pigs were vaccinated twice with the bacterin 1 and nine pigs with the bacterin 2. Ten control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 106.5 colony‐forming units (CFU) of an A. pleuropneumoniae serotype 10 strain. In the bacterin 1 and 2 group, three and two pigs died after inoculation, respectively. Only two pigs of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The percentage of pigs with severe lung lesions (>10% of the lung affected) was 100% in the control group, 70% in the bacterin 1 group and 22% in the bacterin 2 group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 7.0 × 106 CFU/g in the control group, 6.3 × 105 CFU/g in the bacterin 1 group and 1.3 × 106 CFU/g in the bacterin 2 group. It was concluded that both bacterins induced partial protection against severe challenge. Furthermore, there are indications that the bacterin 2, containing A. pleuropneumoniae bacteria grown under conditions resulting in high in vitro adhesin, induced better protection than the bacterin 1. 相似文献
20.
Swine (n = 10) were given a concentrated whole-culture adsorbate bacterin made from group E Streptococcus (GES). Two doses of bacterin were given subcutaneously 3 weeks apart. Control swine (n = 10) were given a blank preparation made from sterile culture medium. Swine were challenge exposed 3 weeks after the 2nd injection of bacterin by being penned continuously for 8 weeks with carrier swine infected with GES. A significant (P less than or equal to 0.009) immune response to vaccination with the bacterin was observed. Vaccinated swine, but not control swine, developed antibodies to an antiphagocytic factor (as detected with bactericidal and long-chain tests) before challenge exposure. Vaccinated swine also developed 51.2% (20 vs 41) fewer abscesses after challenge exposure than did control swine. Control swine developed a greater serologic response to challenge exposure, indicating a more extensive infection with GES. 相似文献