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1.
病原诱导的中间偃麦草ERF转录因子基因的克隆及其表达特性 总被引：1，自引：0，他引：1
应用RT-PCR、RACE技术，从病原诱导的中间偃麦草叶片cDNA中，分离出1个编码ERF基因的全长cDNA序列，该基因暂命名为TiERF1a，编码由292个氨基酸组成的蛋白质，具有ERF转录因子典型的结构，即保守的AP2/ERF DNA 结合域、核定位位点和酸性激活区。TiERF1a的氨基酸序列与一个水稻ERF蛋白OsBIERF3具有66%的同源性，与拟南芥AtERF1同源性仅39.7%，为植物ERF转录因子家族B3亚群的一个新成员。表达分析结果表明，纹枯病菌、赤霉病菌侵染可诱导TiERF1a基因的上调表达，与防卫相关的激素乙烯、茉莉酸也可诱导该基因上调表达，且TiERF1a对外源乙烯、茉莉酸的响应时期早于对纹枯病菌、赤霉病菌响应时期，说明TiERF1a可能通过乙烯、茉莉酸信号途径参与寄主调控对纹枯病菌、赤霉病菌的防御反应。 相似文献
2.
科技期刊编辑应具备的素质刍议 总被引：3，自引：0，他引：3
编辑是科技期刊出版工作的主角，其思维方式左右着科技期刊的总体设计，他对科技期刊出版的全过程、科技期刊的内容质量、论文的取舍起着决定性的作用。本文通过分析编辑对科技期刊出版的影响，指出编辑的素质是影响科技期刊质量的关键，并论述了在新时期下，为了提高科技期刊的质量，科技期刊编辑须具备以下素质：有较高的政治素质及良好的职业道德；有一定的学术水平和相关的专业知识；拥有广博的知识、必要的文学修养；掌握编辑出版专业知识；有一定的社会活动和组织能力等。 相似文献
3.
Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli 总被引：7，自引：0，他引：7
Broccoli is well recognized as a source of glucosinolates and their isothiocyanate breakdown products. Glucoraphanin is one of the most abundant glucosinolates present in broccoli and its cognate isothiocyanate is sulphoraphane, a potent inducer of mammalian detoxication (phase 2) enzyme activity and anti‐cancer agent. This study was designed to measure: glucosinolate levels in broccoli florets from an array of genotypes grown in several environments; the elevation of a key phase 2 enzyme, quinone reductase, in mammalian cells exposed to floret extracts; and total broccoli head content. There were significant environmental and genotype‐by‐environment effects on levels of glucoraphanin and quinone reductase induction potential of broccoli heads; however, the effect of genotype was greater than that of environmental factors. The relative rankings among genotypes for glucoraphanin and quinone reductase induction potential changed, when expressed on a per head basis, rather than on a concentration basis. Correlations of trait means in one environment vs. means from a second were stronger for glucoraphanin and quinone reductase induction potential on a per head basis than on a fresh weight concentration basis. Results of this study indicate that development of a broccoli phenotype with a dense head and a high concentration of glucoraphanin to deliver maximum chemoprotective potential (high enzyme induction potential/glucoraphanin content) is a feasible goal. 相似文献
4.
CIMMYT普通小麦籽粒硬度等位变异的检测 总被引：10，自引：0，他引：10
籽粒硬度主要由5D染色体短臂的一对主效基因Ha控制,研究籽粒硬度等位变异有助于提高小麦的磨粉和食品加工品质。本试验对国际玉米小麦改良中心（CIMMYT）的138份历史品种和代表性高代品系的硬度基因型进行了研究。在用SDS-PAGE鉴定Pina-D1b/Pinb-D1a时,用10%甘油代替水配制分离胶,用PDA代替甲叉配制分离胶和浓缩胶,增强了PINA和PINB两种蛋白带型的分辨率。结果表明,与其他国家硬质麦中Pina-D1a/Pinb-D1b类型偏多的特点明显不同,CIMMYT硬质小麦中puroindoline a（PINA）蛋白缺失类型（或称Pina-D1b/Pinb-D1a）较多,为118个,占85.5%;Pina-D1a/Pinb-D1a（野生型）为11个,占8.0%;Pina-D1a/Pinb-D1b类型有9个,占6.5%。其中,PINA缺失对小麦籽粒硬度的影响最大,与其他2种基因型硬度值之间差异达5%显著水平。先前研究结果表明,PINA蛋白缺失类型的磨粉品质和面包烘烤品质均劣于Pina-D1a/Pinb-D1b类型,因此,建议CIMMYT多引进一些其他硬度变异类型的小麦种质,如Pina-D1a/Pinb-D1b类型等,以改善其硬度基因型过度单一的局面,从而减少PINA蛋白缺失带来的不利影响。同时,也提醒我国以其他用途如抗病、抗旱等为目的,引种CIMMYT小麦时,还应充分考虑PINA蛋白缺失对磨粉和加工品质的不利影响,以更合理引进和有效利用CIMMYT种质资源。 相似文献
5.
栎属4个树种秋冬叶色与生理变化的关系 总被引：2，自引：1，他引：2
为了研究栎类树种秋冬转色期叶色变化的生理机制,以栎属4个树种——鲜红栎(Quercus coccinea)、纳塔栎(Quercus nuttallii)、沈氏栎(Quercus shumardi)、针栎(Quercus palustris)为试材,测定其秋冬转色期叶绿素、类胡萝卜素、花色素苷3种色素和可溶性糖、可溶性蛋白质含量变化。结果表明:叶绿素a、叶绿素b、总叶绿素含量均呈先快后慢的下降趋势;类胡萝卜素含量鲜红栎和沈氏栎呈现下降-上升-下降的趋势,其他2个树种呈平缓下降的趋势;花色素苷含量呈波动上升趋势;叶绿素a与叶绿素b的比值变化趋势前中期较为平缓,后期迅速下降;鲜红栎的可溶性糖含量为单峰曲线,其他3个树种呈双峰曲线,与花色素苷相关性不显著;蛋白质含量与花色素苷含量呈正相关。 相似文献
6.
关于打造农业精品期刊的思考 总被引：3，自引：0，他引：3
详细分析了制约农业精品期刊产生的主要因素,提出通过采取树立品牌意识,明确刊物定位,突出办刊特色,打造一支高素质复合型的人才队伍,发展集团化经营等系列措施,打造农业精品期刊。 相似文献
7.
Summary The process of the separation of gliadin proteins of wheat, using polyacrylamide gel electrophoresis, was studied in detail. It was shown that electrophoresis is a dynamic process during which the pH of the gel changes together with the potassium ion concentration. The most positive effect on the separation of the gliadins was generated by a moving front, which is a boundary between regions with a low concentration of K+ ions and a low pH, and region with a high concentration of K+ ions and a high pH, after optimization of the concentrations of the cations and anions in the electrode solutions. The finding was exploited for the development of an extremely simple electrophoresis system, in which buffers were not needed for obtaining a high resolution. The system was further improved by applying a stacking gel. The advantages of this system are discussed. This new approach may be useful for improving electrophoresis systems for other applications. 相似文献
8.
In order to help tourists choose satisfying scenic spots, content hotels, convenient lines and convenient vehicles, vehicle monitoring control and scheduling management system in a scenic area is presented, which dispatches vehicle by appointments and the statistic of tourists at a sight spot and recommends the tour line by broadcasting in vehicle. Vehicle monitoring control and scheduling management system in a scenic area, a integrative vehicle management system, is an integration of GPS, GIS, trunked radio system, a management system of booking room and a tourist statistic system at a sight spot. 相似文献
9.
Summary The degree of phenotypic similarity of Centana and its near-isogenic lines (NILs) has been investigated. Some NILs have a disease reaction, a gliadin pattern and a yield level which differs from Centana. This means that they still possess many donor genes. One of the NILs, Shortana, must be a derivative of a backcross line crossed with an unknown genotype resulting in a good yielding variety. 相似文献
10.
Godfrey Baldacchino 《Asia Pacific viewpoint》2006,47(1):45-60
Abstract: Rarely does one come across critical analysis which looks at islands as the strategic and candid promoters of a role as political and economic usufructuaries over external resources. This paper is premised on the proposition that a small territory is especially obliged to use extra‐territorial resources as its hinterland for economic success. Such resources extend over a whole range of goods and services and include access to investment, welfare, security, stable currency, international relations, specialised labour power, transfers, markets and higher education. The MIRAB syndrome is one way of articulating this condition. This paper proposes a second cluster of features that are, or can be, deployed by small territories in a manner somewhat different from MIRAB; this second cluster has a more proactive policy orientation and a disposition towards carving out procedural and jurisdictional powers. It is thus proposed to consider a small territory's engagement with the external hinterland as a position on a sliding scale, a strategic mix of options located between two distinct development trajectories, of which MIRAB is one and the PROFIT model the other. 相似文献
11.
Longtao He 《Asia Pacific viewpoint》2023,64(2):279-291
The emergence of a body of work on ethics since the 1990s with a special interest in the self in the Western academia, inspired by Michel Foucault's earlier work, resonates with a concomitant renewed scholarly interest in classical Confucian ethics both in China and internationally. An emphasis on culture and the rejection of a Euro-centric universalist self in these bodies of work accompanies the disavowal of the very possibility of a generic reflexive self. This article seeks to critically examine the ontological positions on the self of these bodies of work, of Foucault's later thoughts, and of classical Confucian ethics. It is argued that there is a theoretical affinity between Foucault's later thoughts and classical Confucian ethics, with both philosophers acknowledging reflexivity as a universally inherited nature of self and making a special consideration of cultural equality and commonality. The examination includes advocacy for a more nuanced approach to notions of cultural equality that relies on targeted research and intercultural dialogue to avoid any predetermining of cultural differences, to allow better-informed appreciation of differences, and to develop a more dynamic conceptualization of culture. This article concludes by initiating a discussion about the design of an integrated approach to cultivate reflexivity through training practices that bring together Foucauldian and Confucian ideas. 相似文献
12.
Classical “high-lysine” sorghum lines are characterized by smaller seeds than average, due to a decrease in prolamin synthesis and a subsequent decrease in yield. To exploit the natural variation in lysine content and to identify ecotypes with a seed lysine content higher than average, characterized by plump seeds, a method was developed based on root-growth inhibition of seeds growing on a medium containing aminoethylcysteine (AEC), a lysine analogue. By using a collection of sorghum mutants and ecotypes a correlation coefficient of 0.926 between root length and lysine content was established. This method, which uses the root length of plants growing on aec to indicate which lines have a potential elevated lysine content, can be applied for the screening of sorghum germplasm. Since this is a non-destructive method it can also be used at the individual seed level, for example for screening progenies of regenerated plants from in vitro culture to exploit the somaclonal variation. 相似文献
13.
为了研究番茄SlYAB2a基因的功能及其在番茄生长发育中的分子机理,以番茄的cDNA为模板,利用RTPCR技术从番茄中克隆到SlYAB2a基因,该基因编码的蛋白质由192个氨基酸残基组成,蛋白质的分子量为21.35k Da,等电点是8.79,平均亲水系数是-0.487。SlYAB2a蛋白质的6~165位氨基酸残基形成Pfam:YABBY结构域,在20~47位有1个C2C2锌指结构域,在122~181位有1个螺旋-环-螺旋结构域。二级结构分析表明SlYAB2a蛋白分子中,α-螺旋占19.79%、β-折叠占14.06%、其余66.15%为不规则卷曲。蛋白多序列比对表明SlYAB2a与马铃薯、林烟草、酿酒葡萄、大豆、拟南芥、甘蓝型油菜、花药野生稻、玉米和小麦YABBY2的一致性分别为98%,78%,71%,65%,64%,64%,58%,57%,57%,说明SlYAB2a蛋白与来源于双子叶植物的YABBY2一致性较高,而与来源于单子叶植物的一致性较低。通过聚类分析发现YABBY2蛋白明显分为单子叶和双子叶2个亚类,SlYAB2a蛋白属于双子叶亚类,与马铃薯、潘那利番茄、绒毛状烟草、林烟草等的YABBY2蛋白序列亲缘关系较近。实时定量RT-PCR分析结果表明,在番茄植物的不同生长发育时期,根、茎、叶、花、果实中,都有SlYAB2a基因表达;并且在花和青熟果实中,SlYAB2a基因表达水平远远高于其他组织。说明SlYAB2a基因在花和果实的发育过程中起着重要作用。洋葱表皮细胞瞬时表达结果表明SlYAB2a蛋白定位于细胞核中。 相似文献
14.
通过常规石蜡切片染色观察，详细研究了糜子胚发育的过程及其时间进程。糜子胚发育的全程包括3个阶段。1.原胚阶段：开花后8-10小时，合子横分裂形成2-细胞胚，再经过一系列有序的细胞分裂，在开花后1天时发育为棒状胚；开花后2天时梨形和弯曲形胚，3天时为蝌蚪形胚。2.器官分化阶段：始于蝌蚪形胚近胎座的一侧细胞液泡化，各 相似文献
15.
为解析2种穗型水稻籽粒蛋白质含量的空间分布特征。以弯曲穗型‘两优培九’和直立穗型‘武香粳14号’为材料,研究了在2个施氮水平下,籽粒蛋白质含量在穗上不同部位和不同粒位间的差异及其分布特征。结果表明:穗内各籽粒在不同蛋白质含量区间的频数变化趋势呈正态分布;在稻穗的同一部位,2个供试品种一次枝梗上的籽粒蛋白质含量高于二次枝梗;一次枝梗上的籽粒蛋白质含量以第2粒位籽粒较低,第1粒位的籽粒较高;二次枝梗上第1粒位籽粒的蛋白质含量较高。蛋白质含量在穗上不同部位及一、二次枝梗上的分布因品种和施氮量的不同而异,施氮量在110~220 kg/hm2范围内,增施氮肥可以减少穗内粒间的籽粒蛋白质含量差异。 相似文献
16.
浅议科技期刊的选题策划 总被引：2，自引：0，他引：2
剖析了影响科技期刊选题策划的三个主要因素:科技期刊二重性,办刊宗旨和读者的需求。指出为了提高科技期刊的质量,充分发挥科技期刊的作用,必须做好科技期刊的选题策划工作,并提出做好科技期刊选题策划工作要突出办刊特色,适应不同读者群体的阅读需要,走市场化的经营道路,选题突出科技期刊的特点,栏目策划要多样化。 相似文献
17.
The urban governance of brownfield restructuring in China: The case of Guangzhou's T.I.T Creative Industry Zone 
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下载免费PDF全文 Over the past several years, the creative reuse of brownfields has played a major role in Chinese cities to give their urban landscape a so‐called second development. This paper analyses the governance of the brownfield restructuring in Guangzhou (Canton). The research is based on a study of the T.I.T Creative Industry Zone, a highly successful creative space currently in danger of demolition. This paper follows DiGaetano and Strom's ( 2003 ) analytical governance framework. This approach allows for deciphering the complexities of decision‐making through a well‐structured set of interdependent categories. In Guangzhou, new policies and institutions have been promoting the restructuring and have contributed to the formalisation of public–private interactions. In general, this indicates a shift towards a more corporatist governance. However, the study of T.I.T reveals a rather intricate picture: the policymaking remains influenced by a top‐down authoritative style – sometimes in an arbitrary manner – and to some extent also by particularistic exchanges. This indicates a complex mode of urban governance consisting of increasingly corporatist elements but still with managerial, and to a lesser extent, clientelistic elements. Finally, the coexistence of different governance modes seems to be a typical outcome of China's transition while still remaining more adaptive, experimental and flexible in nature. 相似文献
18.
Postharvest diseases can cause considerable damage to harvested fruit in controlled atmosphere storage. Since there is a large cost associated with opening the storage rooms, regular assessment of damage levels is not feasible, and many experts agree on the need for a reliable predictive model. Presented here is a simulation model that predicts the overall incidence of disease in a bin of stored fruit as a function of initial infection levels and the fruit's susceptibility to fungal attack. Uninfected fruit tissue, infected fruit tissue, and fungal growth are modelled by a system of three ordinary differential equations. Simulations of the growth and spread of the pathogen in storage were conducted, with disease incidence measured monthly. The model provides insight into the dynamics of postharvest fungal disease, and forms the basis of a predictive model that could be used by packinghouses to determine how long a given crop of fruit can be stored before the infection risk rises above a predetermined tolerable level. 相似文献
19.
为了解决收割冬油菜与栽插早稻的季节矛盾,提前腾地抢插早稻,设计了一种油菜打捆装置,将该装置配置在4SY-2.2型卧式油菜割晒机割台的左侧,组成油菜割捆机。该打捆装置主要由机架、集禾压缩机构、送绳机构、打结器、捆结离合器、推出机构等组成,通过对送绳机构、集禾机构、放捆机构进行设计计算,确定了打捆装置的主要参数。虚拟样机运动仿真分析表明送线机构、打结器、放捆机构动作配合协调,8.95s完成一个工作循环。利用打捆装置样机进行打捆试验,结果表明装置成捆率为95％,油菜捆平均直径为382mm,油菜秆含水率67.5％时每捆平均重量为5.08kg。本研究为油菜的分段收获提供一种新的方案和配套机具。 相似文献
20.
Tatsuro Suzuki Yuji Mukasa Toshikazu Morishita Shigenobu Takigawa Takahiro Noda 《Breeding Science》2012,62(4):360-364
Seed shattering is a significant problem with buckwheat, especially at harvesting time. Several reports have shown that a green-flower mutant of buckwheat, such as W/SK86GF, has a strong pedicel. Although a strong pedicel may provide some resistance to shattering in the field, no study has thoroughly examined this issue. In this paper, we demonstrate that a W/SK86GF has shattering resistance by comparing the degrees of shattering of W/SK86GF and Kitawasesoba (leading variety of Hokkaido with non-green-flower traits) through a test for four years, including a typhoon hit year in the field. In a non-typhoon year, the shattering seed ratio (shattering seed weight/(yield + shattering seed weight) × 100) of W/SK86GF at maturing time +15 days (+15D) was lower than that of Kitawasesoba. In a typhoon hit year, the shattering seed ratios of Kitawasesoba at maturing time and +15D were surprisingly high, 14.4 and 21.1%, respectively. On the other hand, those of W/SK86GF were only 3.08% and 2.57%, respectively; indicating W/SK86GF is promising as a shattering resistant line even in a typhoon hit year. From these results, shattering resistance of W/SK86GF can be evaluated after maturing time such as +15D and pedicel strength would confer W/SK86GF a shattering resistant trait. 相似文献