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1.
An understanding of root system capacity to acquire nitrogen (N) is critical in assessing the long-term growth impact of rising atmospheric CO2 concentration ([CO2]) on trees and forest ecosystems. We examined the effects of mycorrhizal inoculation and elevated [CO2] on root ammonium (NH4+) and nitrate (NO3-) uptake capacity in sweetgum (Liquidambar styraciflua L.) and loblolly pine (Pinus taeda L.). Mycorrhizal treatments included inoculation of seedlings with the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck & Smith in sweetgum and the ectomycorrhizal (EM) fungus Laccaria bicolor (Maire) Orton in loblolly pine. These plants were then equally divided between ambient and elevated [CO2] treatments. After 6 months of treatment, root systems of both species exhibited a greater uptake capacity for NH4+ than for NO3-. In both species, mycorrhizal inoculation significantly increased uptake capacity for NO3-, but not for NH4+. In sweetgum, the mycorrhizal effect on NO3- and NH4+ uptake capacity depended on growth [C02]. Similarly, in loblolly pine, the mycorrhizal effect on NO3- uptake capacity depended on growth [CO2], but the effect on NH4+ uptake capacity did not. Mycorrhizal inoculation significantly enhanced root nitrate reductase activity (NRA) in both species, but elevated [CO2] increased root NRA only in sweetgum. Leaf NRA in sweetgum did not change significantly with mycorrhizal inoculation, but increased in response to [CO2]. Leaf NRA in loblolly pine was unaffected by either treatment. The results indicate that the mycorrhizal effect on specific root N uptake in these species depends on both the form of inorganic N and the mycorrhizal type. However, our data show that in addressing N status of plants under high [CO2], reliable prediction is possible only when information about other root system adjustments (e.g., biomass allocation to fine roots) is simultaneously considered. 相似文献
2.
Red pine and jack pine seedlings growing in styroblocks were inoculated 8 wk after sowing with mycelium/agar slurries of 3 mycorrhizal fungi (Laccaria bicolor, Scleroderma citrinum, and an unidentified basidiomycete), and one suspected mycorrhizal fungus (Cantharellula umbonata). Seedlings inoculated with L. bicolor developed mycorrhizae earlier and in greater numbers than the other inoculation treatments, with red pine out-performing jack pine in both respects. At 34 wk following sowing, seedlings were outplanted on a cleared xeric site in Baraga Co., in Michigan's Upper Peninsula. Seedlings inoculated with C. umbonata failed to form mycorrhizae and were not outplanted. Inoculation treatments did not affect shoot or root weight at outplanting. Red pine inoculated with L. bicolor averaged 21% and 19% greater survival compared with control seedlings after one and two years in the field, respectively. Other inoculation treatments failed to increase seedling survival for either tree species. Jack pine demonstrated higher overall survival than did red pine for both years in all corresponding inoculation treatments. 相似文献
3.
We compared the effects of two auxin transport inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA)) on rhizogenesis and mycorrhizal establishment of Picea abies L. (Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L. bicolor under in vitro conditions strongly increased host root and shoot growth. Although TIBA had no effect on taproot growth, NPA decreased taproot growth and deformed the root apex into a globular shape in both non-inoculated seedlings and seedlings inoculated with L. bicolor. Inoculation with L. bicolor strongly increased lateral rhizogenesis of the seedlings, and application of 100 microM indole-3-acetic acid (IAA) partially reproduced this effect. Although TIBA completely inhibited the stimulatory effect of L. bicolor on lateral root formation, NPA inhibited it only partially. Both TIBA and NPA counteracted the effect of exogenous IAA on lateral rhizogenesis. Inoculation with L. bicolor significantly increased shoot growth and seedling dry biomass, whereas application of exogenous IAA had no effect on either parameter. There was no effect of NPA on shoot growth and biomass production. The presence of TIBA completely prevented the development of ectomycorrhizal structures (mantle and Hartig net). In the presence of NPA, the number of seedlings colonized by the fungus was reduced and the degree of development of ectomycorrhizal structures was variable, but not completely prevented. In medium lacking tryptophan, neither TIBA nor NPA inhibited the release of IAA produced by L. bicolor in pure culture. When 100 microM tryptophan was added to the medium, TIBA significantly increased the amount of IAA released by the fungus, whereas NPA had no significant effect. We conclude that fungal IAA plays an important role in plant rhizogenesis and in the establishment of ectomycorrhizal symbiosis. 相似文献
4.
Growth and nutrient uptake of ectomycorrhizal Pinus sylvestris seedlings in a natural substrate treated with elevated Al concentrations 总被引：1，自引：0，他引：1
Models of the effects of elevated concentrations of aluminum (Al) on growth and nutrient uptake of forest trees frequently ignore the effects of mycorrhizal fungi. In this study, we present novel data indicating that ectomycorrhizal mycelia may prevent leaching of base cations and Al. Mycorrhizal and non-mycorrhizal Pinus sylvestris L. seedlings were grown in sand obtained from the B-horizon of a local forest. In Experiment 1, non-mycorrhizal seedlings and seedlings inoculated with Hebeloma cf. longicaudum (Pers.: Fr.) Kumm. ss. Lange or Laccaria bicolor (Maire) Orton were provided with nutrient solution containing 2.5 mM Al. Aluminum did not affect growth of non-mycorrhizal seedlings or seedlings inoculated with L. bicolor. Seedlings colonized by H. cf. longicaudum had the highest biomass production of all seedlings grown without added Al, but the fungus did not tolerate Al. Shoots of seedlings colonized by L. bicolor had the lowest nitrogen (N) concentrations but the highest phosphorus (P) concentrations of all seedlings. The treatments had small but significant effects on shoot and root Al concentrations. In Experiment 2, inoculation with L. bicolor was factorially combined with the addition of a complete nutrient solution, or a solution lacking the base cations K, Ca and Mg, and solutions containing 0 or 0.74 mM Al. Seedling growth decreased in response to 0.74 mM Al, but the effect was significant only for non-mycorrhizal seedlings. Mycorrhizal seedlings generally had higher P concentrations than non-mycorrhizal seedlings. Aluminum reduced P uptake in non-mycorrhizal plants but had no effect on P uptake in mycorrhizal plants. Mycorrhizal colonization increased the pH of the soil solution by about 0.5 units and addition of Al decreased the pH by the same amount. We conclude that the presence of ectomycorrhizal mycelia decreased leaching of base cations and Al from the soil. 相似文献
5.
The ectomycorrhizal fungus Laccaria bicolor S238 N and the bacterium Pseudomonas fluorescens BBc6 were used separately and in combination to induce in vitro rooting of de-rooted shoot hypocotyls of Norway spruce (Picea abies (L.) Karst.). When the culture medium was supplemented with tryptophan, a precursor of indole-3-acetic acid (IAA) synthesis, the presence of the ectomycorrhizal fungus increased the percentage of hypocotyls forming roots; furthermore, both the fungal and bacterial inoculations enhanced the number of roots formed per rooted hypocotyl. Similar results were obtained by adding exogenous IAA (5 and 10 &mgr;M) to the rooting medium. After the rooting phase, the fungal inoculation enhanced adventitious root elongation and branching as well as the aerial growth of the cuttings. Pseudomonas fluorescens BBc6 had no effect on these parameters. The production of IAA by pure cultures of L. bicolor S238 N and P. fluorescens BBc6 was estimated by immunochemical analysis using specific anti-IAA antibodies. Both L. bicolor S238 N and P. fluorescens BBc6 synthesized IAA in pure culture and synthesis was stimulated in the presence of tryptophan. Thus, the effect of the fungus in stimulating adventitious root formation and subsequent elongation and branching can be attributed, at least partially, to the synthesis of IAA by the fungus. The finding that P. fluorescens BBc6 had no effect on root elongation and branching although it produced IAA suggests that either IAA was not the only parameter involved in the stimulation of these processes by L. bicolor S238 N or the bacterium produced other compounds that counteracted the stimulatory effects of IAA on root elongation and branching. 相似文献
6.
The field performance of conifer and hardwood species inoculated with different inoculation treatments was evaluated 5 or
3 years after outplanting in the field trials established in the Canadian Prairie Provinces. In conifer trials, the growth
of white spruce, black spruce, lodgepole pine, and larch seedlings observed on different sites varied greatly to different
inoculation treatments depending on plant and fungal species involved. Five years after outplanting, most of the introduced
fungi were replaced by several indigenous ectomycorrhizal fungal species except for Laccaria bicolor strain. Survival rates of most of the inoculated seedlings were not significantly different from control seedlings naturally
colonized by other resident fungi. In hardwood trial, the effects of nursery inoculation of different poplar clones, aspen
and balsam poplar were very limited. Growth and survival data were combined into seedling volume and plot volume index (PVI)
for measurement of total growth response of seedlings. Our results demonstrated that certain inoculated plant-fungus combinations
have played a positive role during the initial establishment of these seedlings in the field, which was reflected on significantly
greater stem volume and PVI compared to non-inoculated control seedlings. The results from this study provided useful information
on field evaluation for potential benefits of mycorrhizal inoculation in nursery. 相似文献
7.
接种苗木在生长量和生物量上均表现出极其显著的生长优势,ECM真菌蜡蘑菌单接种及其与4种VA菌根菌剂混合接种,对苗木生长的促进作用尤为显著。在接种后16周时,与对照苗相比,蓝桉和尾叶桉接种苗木的高生长量最大增幅分别为28.86%(LS)和86.65%(LG);两种桉树地上部分平均干质量最大增幅分别为129.93%(LS)和133.34%(L),地下部分分别为119.93%(LF)和174.83%(LG)。桉树对不同菌根菌及其组合的菌根依赖性表现出很大的差异,对混合接种的真菌组合有相对高的菌根依赖性,而对土壤菌剂依赖性很小。菌根感染与苗木生物量间有显著的相关性。试验结果还证实了P素对桉苗生长的促进作用,P素水平对菌根的接种效应有一定的影响。 相似文献
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10.
We studied the effects of phosphate fertilization and inoculation with the arbuscular mycorrhizal fungi Glomus mosseae (Nicol. and Gerd.) Gerdmann and Trappe, Glomus intraradices Schenck and Smith or Glomus viscosum Nicolson on shoot apical growth of plantlets that had been micropropagated from MM 106 apple (Malus pumila L.) and Mr.S. 2/5 plum (Prunus cerasifera Ehrh.) rootstocks. Unfertilized and non-mycorrhizal plantlets showed no apical growth during the post in vitro acclimation phase, whereas P fertilization induced early resumption of shoot apical growth. Growth enhancement and percentage of actively growing apices of mycorrhizal-inoculated plantlets were comparable to those obtained in plantlets fertilized with P. Furthermore, tissue P concentrations of mycorrhizal plantlets were similar to those of plantlets fertilized with P. We conclude that mycorrhizal inoculation can be used as a biotechnological tool to overcome blocked apical growth and to reduce chemical inputs, especially P inputs, to micropropagated fruit trees. 相似文献
11.
We studied the effects of ectomycorrhizal colonization by Laccaria bicolor (Maire) Orton S238 and Paxillus involutus (Batsch) Fr. 533 on cadmium (Cd) toxicity in Norway spruce seedlings (Picea abies (L.) Karst.). Both mycorrhizal and nonmycorrhizal seedlings were exposed to 0 (control), 0.5 or 5 &mgr;M CdSO(4) for 9 weeks in a sand culture system with frequent addition of nutrient solutions. In pure culture, P. involutus and L. bicolor showed similar Cd tolerance. However, in symbiosis, the Cd treatments decreased colonization by L. bicolor but not by P. involutus. Paxillus involutus ameliorated the negative effects of 0.5 &mgr;M Cd on shoot and root growth and chlorophyll content of old needles, whereas L. bicolor did not. Mycorrhizal colonization did not affect Cd concentrations of old needles and roots of seedlings. Despite differences between the ectomycorrhizal fungi in colonization and ability to alleviate Cd toxicity of seedlings, both species reduced Cd concentrations of young needles to a similar degree compared with nonmycorrhizal seedlings. However, in the 0.5 &mgr;M Cd treatment, the Cd content of needles of seedlings colonized by P. involutus was increased, whereas the Cd content of needles of seedlings colonized by L. bicolor was similar to that of needles of nonmycorrhizal seedings. When the amount of Cd translocated to needles was expressed on a root length basis to account for differences in the size of the root systems, the amount of Cd translocated to the needles was similar in seedlings mycorrhizal with P. involutus and in nonmycorrhizal seedlings. All mycorrhizal seedlings were similarly affected by 5 &mgr;M Cd, indicating that the amelioration efficiency of ectomycorrhizal fungi is dependent on the metal concentration to which the roots are exposed. Concentrations of P, K, Ca, Mg and Mn were decreased by 5 &mgr;M Cd to a similar extent in both nonmycorrhizal and mycorrhizal seedlings. In contrast to L. bicolor, P. involutus increased P uptake and altered patterns of root branching. We conclude that mycorrhizas alleviate Cd-induced reductions in growth of Picea abies seedlings. Although the two mycorrhizal fungi examined differed in their ability to alleviate Cd toxicity, these differences were not related to differences in Cd uptake or translocation to the shoot of the mycorrhizal seedlings. We suggest that amelioration of Cd toxicity by P. involutus may be a result of improved P nutrition or changes in root morphology, or both. 相似文献
12.
In vitro syntheses of ectomycorrhizas of Pinus patula, P. pseudostrobus, P. oocarpa and P. elliotii with Amanita muscaria, Laccaria fraterna, L. laccata, Pisolithus tinctorius, Rhizopogon luteolus, Scleroderma citrinum and Thelephora terrestris were attempted. Most of the fungi initiated mycorrhizal formation within 2 weeks after inoculation. Laccaria fraterna and S. citrinum formed mycorrhizas after 6 weeks of inoculation. Amanita muscaria failed to form mycorrhizas during the 12 week period of observation. The degree of colonization of different mycorrhizal fungi varied with different species of Pinus. 相似文献
13.
为筛选乡土用材树种与菌根型食用红菇菌的高效共生组合体,以红锥Castanopsis hystrix、白锥Castanopsis fabric和马尾松Pinus massoniana为宿主,以正红菇Russula vinosa07240、07242、07243、0204和0553菌株和鳞盖红菇Russulalepida0005菌株为菌种,开展了苗期接种试验。结果表明,参试的6个红菇菌株均可在红锥、白锥和马尾松苗木根系上形成感染,感染率46.7%～90.0%。马尾松的接种效应最快,不同接种处理之间苗高和地径的生长差异分别在接种后1个月和4个月时达到显著;而红锥和白锥苗高生长差异则在接种后10个月,地径生长差异分别在接种后12个月和14个月时达到显著。以0553菌株接种的红锥、马尾松苗高、地径及总生物量高于其他5个菌株接种处理。与对照相比,0553菌株接种下的红锥苗高、地径及总生物量增长量分别达24.1%、19.8%和50.4%,马尾松则分别达20.9%、18.6%和49.1%。对于白锥,则以07242菌株接种处理下的生长效应最大,苗高、地径及总生物量增长量分别达54.0%、20.2%和57.2%。红锥、马尾松与正... 相似文献
14.
Korean spruce(Picea koraiensis Sieb. El Zucc.)is one of the main afforestation species in northern China.Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine(P. sylvestris var. mongolica) seedlings brought by the plant beneficial fungus Trichoderma virens(J.H. Mill., Giddens and A.A. Foster) Arx and ectomycorrhizal fungus(Suillus luteus(L.) Roussel.) can also benefit Korean spruce seedlings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots,biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T.virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P.sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S.luteus to produce-1,3-glucanase and chitinase. This inoculation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results. 相似文献
15.
Yoshitaka Ono Zinaida M. Azbukina Makoto Kakishima Shigeru Kaneko 《Journal of Forest Research》1997,2(4):227-231
The larch brown rust caused byTriphragmiopsis laricinum was found onLarix cajandri in Primorsky Territory, Russia. This was a new host and locality record for the pathogen. Preliminary survey of the disease
incidence in several localities in East and Northeast Russia and Yakutia-Sakha Republic indicated the restricted distribution
of the rust in a few localities in Primorsky Territory. As previously reported in China,T. laricinum was proved to form uredinia in nature and to be able to propagate vegetatively by urediniospores. This suggests the fungus
could become a devastating pathogen threatening a large-scale larch plantation in Far East of Russia. Field observations and
the failure of basidiospore inoculation onto larch needles suggest the heteroecious life cycle ofT. laricinum. 相似文献
16.
Meng Fanrong 《林业研究》1995,6(2):45-49
A large areas of Larch seedlings, including container and bare-root seedlings (new and transplanted seedlings) were inoculated
pure inocula that were cultivated by usingSuillus grevillei. Compared with those of control area, all growth indices as rate of emergence, growth(in seedling height, collar diameter
and number of lateral branch-root), rate of mycorrhiza inoculum, rate of survival and content of nutrient elements were greatly
increased. Therefore,Suillus grevillei is a better and suitable mycorrhizal fungus to larch seedling either in the severe cold mountain area or in the plain region.
It should be spread and used in larch cultivation and planting. 相似文献
17.
Amornrat Chaiyasen David D. Douds Paiboolya Gavinlertvatana Saisamorn Lumyong 《New Forests》2017,48(4):547-562
Regeneration of stands of valuable tropical hardwood tree species for sustainable harvest requires production of seedlings with high probabilities of survival. One way to enhance the vigor of plants for outplanting is pre-colonization of roots by arbuscular mycorrhizal (AM) fungi. We pursued the strategy that the most promising AM fungus candidates for inoculation would be those associated with the tree of interest in the field. AM fungus communities were assessed in five plantations of Tectona grandis Linn.f. A total of 18 AM fungal morphotypes were found, representing four families: Glomeraceae (49.6%), Acaulosporaceae (24.9%), Claroideoglomeraceae (20.8%), and Gigasporaceae (4.8%). AM fungus spore density was negatively correlated with soil organic carbon. Some of these AM fungi, plus Rhizophagus irregularis, were established in pot culture and in vitro with transformed carrot roots, and subsequently used to inoculate micropropagated plantlets of T. grandis. Tectona grandis plantlets inoculated in vitro were successfully colonized by all AM fungi studied. Plants inoculated with Funneliformis mosseae were taller than uninoculated plants. Tectona grandis plantlets inoculated with the AM fungus Claroideoglomus etunicatum PBT03 were taller than uninoculated controls in ex vitro experiments. This study provides early insight for the targeted use of the AM symbiosis in production of important tree species in future greenhouse studies and reforestation. 相似文献
18.
We tested the effects of ectomycorrhizal (ECM) inoculation on greenhouse-grown white spruce (Picea glauca (Moench) Voss) and jack pine (Pinus banksiana L.) seedlings to be used for revegetation of salt-affected tailing sands resulting from the exploitation of oil sand in northeastern Alberta, Canada. White spruce and jack pine seedlings were inoculated with three ECM fungi selected for their in vitro tolerance to excess Na+ and Cl-: Hebeloma crustuliniforme (Bull) Quel. UAMH 5247, Laccaria bicolor Maire (Orton) UAMH 8232 and a Suillus tomentosus (Kauff.) Sing., Snell and Dick isolate from a salt-affected site. The physiological responses of the seedlings to a gradient of NaCl concentration (0, 50, 100 and 200 mM) were assessed over four weeks by: (1) Na+ accumulation and allocation; (2) chlorophyll a fluorescence; (3) growth, (4) water content; and (5) organic osmolyte accumulation. Jack pine seedlings were more sensitive than white spruce seedlings to increasing Na+ and Cl- concentrations. Both species showed decreasing biomass accumulation, and increasing concentrations of organic osmotica and Na with increasing NaCl concentration. White spruce seedlings inoculated with the S. tomentosus isolate had the best growth response at all NaCl concentrations tested. Although jack pine seedlings inoculated with the L. bicolor or S. tomentosus isolate exhibited the highest growth in the 50 and 100 mM NaCl treatments, both fungi increased the photochemical stress and dehydration of their hosts in the 200 mM NaCl treatment. At the latter concentration, jack pine seedlings inoculated with H. crustuliniforme showed the greatest tolerance to salt stress. Although the different fungi altered the physiological response of the host in different ways, inoculation with salt-stress-tolerant ECM fungi increased growth and reduced the negative effects of excess NaCl. Use of controlled mycorrhization may increase survival of coniferous seedlings used for revegetation of salt-affected sites. 相似文献
19.
Wang Xiao-xiong Lu Long-dou Hao Huai-qing Teng Nian-jun Chen Tong Guo Yi-ming Yang Ying-gen Guo Zhong-chen Lin Jin-xing 《中国林学(英文版)》2007,9(3):182-188
A high-efficiency somatic embryogenesis protocol of Japanese larch (Larix leptolepis Gordon) has been established in our investigation. Calli were induced from immature zygotic embryos of female cones of L. leptolepis and then subcultured regularly on to a modified Gupta and Durzan (DCR) basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur-ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid (ABA) com-pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media 相似文献
20.
Chunjie Tian Xingyuan He Yang Zhong Jiakuan Chen 《Forest Ecology and Management》2002,170(1-3):307-312
Through biological inoculation technology, the joint symbiosis of Tibetan seabuckthorn (Hippophae tibetana) in pure culture was identified and the effects of dual inoculation with Frankia and mycorrhizal fungi on the host plants in pot cultures were investigated. The results obtained from the comparative study showed that H. tibetana could form nodules and VA mycorrhiza both in pot and pure cultures. VA mycorrhizae and Frankia can stimulate the growth and the nitrogen fixation ability of host plants, respectively, yet the stimulation of the dual inoculation on the growth and nitrogen fixation ability of the host plants was more significant (p<0.05): stronger nitrogen-fixing ability, higher VA mycorrhizal development and better growth of seedlings in VAH and HR16 dual inoculation. 相似文献