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1.
番茄褪绿病毒Tomato chlorosis virus(ToCV)是一种由烟粉虱Bemisia tabaci传播的正义单链RNA病毒,在田间常与番茄黄化曲叶病毒Tomato yellow leaf curl virus(TYLCV)复合侵染而造成番茄生产上重大的经济损失。为了明确ToCV与TYLCV的复合侵染对烟粉虱传播ToCV所造成的影响,本文采用RT-PCR以及qRT-PCR检测了复合侵染的番茄对烟粉虱获取和传播ToCV的影响。研究表明,烟粉虱取食复合侵染的番茄后对ToCV的传播效率显著提高,仅25头烟粉虱的传毒率即可达到100%,ToCV在烟粉虱以及番茄体内的累积量均显著提高。说明这种复合侵染促进了烟粉虱对ToCV的传播,在田间应当及时防控烟粉虱,警惕病毒与烟粉虱的蔓延。 相似文献
2.
研究烟粉虱传播番茄褪绿病毒Tomato chlorosis virus (ToCV)的发生规律,建立其预测预报模型,能够指导田间早期有效防治。本研究于2014年－2018年每年采集山东寿光蔬菜基地大棚番茄和杂草的植株叶片,并收集植株上携带的所有烟粉虱,以健康番茄、杂草叶片和室内饲养的健康烟粉虱为阴性对照;实验室ToCV侵染性克隆接种的感病叶片以及从感病叶片上获毒的烟粉虱为阳性对照,将采集的样品带回实验室进行检测和鉴定等试验。根据病情发生规律,建立了番茄褪绿病毒病的预测预报模型,模拟所得方程为:Y=2.570+0.089X4-7.548X7,其中Y为11月份番茄褪绿病毒病发生率,X4为采集的样品上平均每株所携带的烟粉虱数量,X7为采集的田间杂草的带毒率。预测模拟结果显示,烟粉虱数量以及杂草的带毒率与番茄褪绿病毒病的发生率极显著正相关,回归检测结果历史符合率为96.8%以上。明确了影响番茄褪绿病毒病发生的影响因子,基于烟粉虱数量以及杂草的带毒率,构建了病害预测预报模型。研究结果有助于及时发现番茄褪绿病毒病并采取相应预防措施。 相似文献
3.
为有效控制日光温室番茄褪绿病毒病,于2014—2015年通过RT-PCR检测方法研究了济南市日光温室番茄褪绿病毒(Tomato chlorosis virus,ToCV)的发生规律、其与Q型烟粉虱Bemisia tabaci种群动态的关系及防虫网对该病毒病的防控效果。结果表明,春季日光温室番茄植株上Q型烟粉虱成虫数量呈增长趋势,5月下旬最高达到0.10头/叶,秋季日光温室番茄植株上Q型烟粉虱成虫数量9月上旬达最高7.42头/叶,后逐渐下降;日光温室Q型烟粉虱带毒率随着定植时间的延长而逐渐上升,之后维持相对稳定状态,即春季为20.00%~24.14%,秋季为30.00%~40.00%。日光温室ToCV发生与Q型烟粉虱成虫数量和带毒率密切相关,春季番茄最高发病率为12.00%;秋季番茄植株最高发病率为93.02%。番茄育苗和生长期用100目防虫网隔离可显著降低番茄植株带毒率。因此,秋季是日光温室ToCV防控关键期,覆盖防虫网阻隔烟粉虱可有效防治ToCV,推荐在日光温室使用。 相似文献
4.
为明确烟粉虱传播的番茄褪绿病毒(Tomato chlorosis virus,ToCV)与番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)对不同番茄品种的复合侵染情况,于2015年11月在山东省寿光市温室内采集13个番茄品种共390份疑似发病植株叶片,对不同番茄品种的TYLCV抗性和2种病毒的复合侵染以及温室内发病番茄植株上烟粉虱成虫的带毒率进行检测。结果表明,采集的13个番茄品种经分子标记检测鉴定均为TYLCV杂合抗性;不同番茄品种ToCV与TYLCV的复合侵染率存在明显差异,大果番茄粉宴和贝瑞上复合侵染率最高可达73.3%,而樱桃番茄八喜上未检测到这2种病毒的复合侵染。此外,在发病番茄植株上采集的烟粉虱成虫体内可检测到2种病毒,其中烟粉虱ToCV带毒率为90.7%,TYLCV带毒率为80.0%,同时检测到ToCV与TYLCV的概率为71.3%。表明ToCV和TYLCV的复合侵染在山东省番茄生产中普遍发生,烟粉虱可同时携带这2种病毒并广泛传播。 相似文献
5.
番茄褪绿病毒tomato chlorosis virus (ToCV)是我国蔬菜生产的重要新发病毒,其寄主范围逐年扩大。2019年10月项目组在山东寿光西葫芦温室调查时发现部分叶片呈现黄化、脉间褪绿,类似于ToCV侵染症状,并伴有烟粉虱发生。利用特异性引物检测发现,扩增的目的片段与GenBank中登录的侵染番茄的ToCV基因序列(登录号：KC887998.1)同源性高达99.58%,充分说明西葫芦植株已被ToCV侵染。通过对病毒病发生规律和烟粉虱虫口数量调查发现,西葫芦定植后1个月表现侵染症状的植株为2.0%,定植后2个月达到4.2%,定植后4个月后高达68.2%,病毒发生呈指数增长,而烟粉虱虫口数量却维持较低密度。从济南和德州采集的西葫芦疑似病叶和烟粉虱中也检出ToCV病毒,说明该病毒可能已经在山东省设施西葫芦主要种植区普遍发生,并经烟粉虱广泛传播,需引起高度重视。 相似文献
6.
番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV)是一种由烟粉虱传播的单链环状DNA病毒, 在田间可与多种病毒发生复合侵染, 如番茄褪绿病毒(tomato chlorosis virus, ToCV)等?本文对比了TYLCV单独侵染和TYLCV与ToCV复合侵染对烟粉虱获取和传播TYLCV的影响?结果表明, 与取食TYLCV单独侵染的番茄相比, 取食复合侵染番茄的烟粉虱对TYLCV的传毒率显著提高, 且番茄植株和烟粉虱体内TYLCV的病毒积累量也显著提高?试验结果说明复合侵染会提高烟粉虱的传毒率, 促进TYLCV的发生与流行? 相似文献
7.
番茄褪绿病毒Tomato chlorosis virus(ToCV)对我国番茄种植业破坏极大,而粉虱类昆虫是其主要传播介体。本研究调查了我国内蒙古自治区赤峰市和贵州省贵阳市的番茄褪绿病毒病发生情况,发现赤峰市的发病率为73.3%,贵阳市的发病率为53.3%。两个地区均存在Q型烟粉虱和温室白粉虱,赤峰市的Q型烟粉虱和温室白粉虱分别占75.9%和24.1%;贵阳市的Q型烟粉虱和温室白粉虱分别占39.8%和60.2%。对两个地区的两种粉虱种群在室内进行传毒特性研究,结果发现,经过48 h获毒后Q型烟粉虱和温室白粉虱的获毒量没有明显差异,但无论1头或10头粉虱,Q型烟粉虱的传毒量都高于温室白粉虱。结果说明,近年来随着Q型烟粉虱发生的日益严重,番茄褪绿病毒通过Q型烟粉虱的传播值得人们重视和进一步研究。 相似文献
8.
为明确Q型烟粉虱Bemisia tabaci成虫对番茄褪绿病毒(Tomato chlorosis virus,ToCV)的传毒特征,应用RT-PCR技术检测了ToCV在Q型烟粉虱成虫体内的存留时间、Q型烟粉虱成虫对ToCV的获毒效率及传毒效率。结果表明,Q型烟粉虱成虫离开带毒番茄植株后,其带毒率随时间的延长逐渐降低,最初为85.73%,96 h后仅为17.14%。在相同温度下,Q型烟粉虱成虫的获毒率先随着取食时间的延长而提高,而后处于相对稳定状态,当温度为21、26、31℃时,Q型烟粉虱成虫获毒率达到峰值的时间分别为72、48、6 h,最高获毒率分别为56.52%、54.17%和61.64%。当温度为26℃时,Q型烟粉虱成虫的传毒效率随着取食时间的延长而提高,取食时间为0.5 h时,番茄植株带毒率为25.00%,取食时间为48 h时,番茄植株带毒率达91.67%;温度对Q型烟粉虱成虫的传毒效率有极显著影响,取食48 h后,在21~31℃条件下番茄植株带毒率为83.33%~91.67%,在16℃条件下仅为16.67%。表明Q型烟粉虱成虫对ToCV具有较强的获毒和传毒能力,对其进行有效控制可以防止ToCV的扩散和蔓延。 相似文献
9.
侵染甜椒的番茄褪绿病毒的分子鉴定 总被引：2，自引：0，他引：2
番茄褪绿病毒（Tomato chlorosis virus, ToCV）是一种由粉虱传播的RNA病毒,可以侵染番茄和辣椒等常见蔬菜,已在世界多地造成严重危害。2012年在北京市大兴区调查蔬菜病毒病时发现保护地栽培的甜椒植株表现脉间褪绿症状,且植株上烟粉虱数量多,初步推测为ToCV危害。通过提取显症样品总RNA,利用ToCV特异性引物进行反转录PCR,扩增得到463 bp的目标条带。通过测序和核苷酸序列比对分析,表明该序列与国外已报道的ToCV HSP70h（the heat shock protein 70 homolog,热激蛋白70家族）基因序列相似性为99%。这是对ToCV在我国侵染甜椒的首次报道。 相似文献
10.
2012年秋季, 在山东泰安番茄主要种植区中采集到叶片褪绿, 叶脉颜色变深的疑似番茄褪绿病毒病和番茄侵染性褪绿病毒病的番茄样品。利用番茄褪绿病毒(Tomato chlorosis virus, ToCV)的特异引物 ToCV1/ToCV2和番茄侵染性褪绿病毒(Tomato infectious chlorosis virus, TICV)的特异引物TICV1/TICV2分别对样品进行扩增, 最后仅得到利用引物 ToCV1/ToCV2 扩增的101 bp 的核苷酸序列, 对该核苷酸序列克隆并测序。序列比对表明, 山东泰安地区分离物与已登录的番茄褪绿病毒(ToCV)分离物相似性都在99%以上。随后, 对山东泰安种植区ToCV番茄分离物进行外壳蛋白(CP)及热激蛋白(HSP70)序列的扩增、克隆和测序(GenBank登录号KC812620/KC812625), 经NCBI BLAST比对发现, 目的序列与番茄褪绿病毒日本番茄分离物ToCV Japan/Tochigi (GenBank登录号AB513442/AB513443)相似性最高为99%, 同属于毛型病毒属的番茄褪绿病毒, 这是首次明确山东地区番茄受到番茄褪绿病毒的侵染。 相似文献
11.
Tomato chlorosis virus (ToCV) is a plant virus that is mainly propagated by Bemisia tabaci in a semi-persistent and non-circulative manner.It has a wide range of host plants,and has been reported in many countries,causing serious economic losses in vegetable production.In 2019,we investigated about 10 fields,one ha each in Shouguang,Shandong province (China),and in each field we observed symptoms of interveinal chlorosis on lower leaves of the Solanum torvum Swartz,and a large number of B.tabaci gathered on the back of its leaves.To determine the presence of ToCV,total RNA of S.torvum was extracted followed by RT-PCR.The 1 074 (GenBank accessions number MN545620) and 466 bp (GenBank accessions number MN545621) fragments were gel purified and sequenced.The sequences shared 99.44% and 99.57% similarity with ToCV reference sequence tomato chlorosis virus segment RNA1 (AY903447) and RNA2 (AY903448).The results of insect transmission test confirmed that ToCV can spread from S. torvum to tomato.This study confirms S.torvum as a newly reported host of ToCV. 相似文献
12.
Tomato chlorosis virus (ToCV) is a plant virus that is mainly propagated by Bemisia tabaci in a semi-persistent and non-circulative manner.It has a wide range of host plants,and has been reported in many countries,causing serious economic losses in vegetable production.In 2019,we investigated about 10 fields,one ha each in Shouguang,Shandong province (China),and in each field we observed symptoms of interveinal chlorosis on lower leaves of the Solanum torvum Swartz,and a large number of B.tabaci gathered on the back of its leaves.To determine the presence of ToCV,total RNA of S.torvum was extracted followed by RT-PCR.The 1 074 (GenBank accessions number MN545620) and 466 bp (GenBank accessions number MN545621) fragments were gel purified and sequenced.The sequences shared 99.44% and 99.57% similarity with ToCV reference sequence tomato chlorosis virus segment RNA1 (AY903447) and RNA2 (AY903448).The results of insect transmission test confirmed that ToCV can spread from S. torvum to tomato.This study confirms S.torvum as a newly reported host of ToCV. 相似文献
13.
Tomato chlorosis virus: a new whitefly-transmitted, Phloem-limited, bipartite closterovirus of tomato 总被引：1，自引：0，他引：1
ABSTRACT Tomato chlorosis virus (ToCV) is the second whitefly-transmitted, phloem-limited, bipartite closterovirus described infecting tomato. ToCV is distinct from tomato infectious chlorosis virus (TICV), based on lack of serological and nucleic acid cross-reactions and differences in vector specificity. TICV is transmitted only by the greenhouse whitefly (Trialeurodes vaporariorum), whereas ToCV is transmitted by the greenhouse whitefly, the banded-wing whitefly (T. abutilonea), and Bemisia tabaci biotypes A and B (B. argentifolii). Double-stranded (ds) RNA analyses of ToCV show two prominent dsRNAs of approximately 7,800 and 8,200 bp, with several small dsRNAs. Digoxigenin-11-UTP-labeled riboprobes derived from cDNA clones representing portions of RNAs 1 and 2 were used in Northern blot hybridizations to detect two large nonhomologous dsRNAs and a subset of smaller dsRNAs. These probes were used in dot blot hybridizations to detect ToCV in infected tomato. Inclusion bodies and cytoplasmic vesicles were consistently observed in phloem tissues of ToCV-infected Nicotiana clevelandii. Computer-assisted sequence analysis showed significant homology between ToCV clones that hybridize specifically with RNAs 1 and 2 and the lettuce infectious yellows virus methyltransferase of RNA 1 and the HSP70 heat shock protein homolog of RNA 2, respectively. Thus, ToCV is another member of the growing subgroup of bipartite closteroviruses transmitted by whiteflies. 相似文献
14.
Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) are two criniviruses that are emerging worldwide, and induce similar yellowing diseases in tomato crops. While TICV is transmitted only by Trialeurodes vaporariorum , ToCV is transmitted by three whitefly species in two genera Trialeurodes vaporariorum , T. abutilonea and Bemisia tabaci . The efficiency of transmission by T. vaporariorum from plants infected by one virus or by both was compared, and the probability of virus transmission by a single whitefly was derived from group testing experiments. The estimated transmission probabilities ranged from 0·01 to 0·13, and were not significantly different between ToCV and TICV, or between single and mixed infections. Experiments using B. tabaci as a vector and source plants infected by TICV and ToCV did not reveal any functional trans-complementation for transmission of TICV by ToCV, suggesting that if this phenomenon occurs in nature, it is at a very low frequency. Possible reasons why TICV did not establish in southern France while ToCV is now endemic are discussed. 相似文献
15.
番茄黄化曲叶病毒(Tomato yellow leaf curl virus, TYLCV) 及番茄褪绿病毒(Tomato chlorosis virus, ToCV)是危害番茄的主要病毒。2016~2017年,在河北省18个番茄主产区调查番茄病毒病危害情况,采集262份植株矮化、叶片黄化、褪绿、卷曲的番茄样品,利用分子生物学技术对病原进行鉴定。结果表明:2016~2017年河北省番茄病毒病发生普遍,所检样品中TYLCV的侵染率为68.66%,其中与ToCV复合侵染率为19.5%;除栾城、滦南、乐亭、永年4地样品暂未检测到ToCV外,其他14个采样点的样品均检测到ToCV,侵染率为19.5%,且全部表现为与TYLCV复合侵染,河北省番茄主产区暂未发现ToCV单独侵染的样品。 相似文献