共查询到19条相似文献，搜索用时 234 毫秒
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应用ＧＣ－ＭＳ、ＰＧＣ－ＭＳ、ＥＳＲ和ＩＲ等分析方法测定了α－蒎烯等离子体聚合过程中气相产物的组成及其聚合物结构。根据分析结果，提出α－蒎烯等离子体聚合机理是自由基聚合反应机理。 相似文献
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以１５个银杏主要栽培品种的种子胚乳为材料,利用筛选出来的１２种具多态型的引物进行ＲＡＰＤ分析．结果表明,１５个品种之间在分子水平上存在很大差异,并确定了这些品种的ＲＡＰＤ标记基因型．以标记基因型为依据,可以利用单倍体种子胚乳和二倍体叶片组织在ＤＮＡ水平上对这些品种进行有效的鉴别．依据各个品种的标记基因型对这些品种进行分子聚类的结果与传统的三大类分类法非常吻合． 相似文献
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以16个银杏栽培品种的单倍体胚乳DNA为材料,用筛选出了高效引物进行RAPD分析,确定了各品种的标记基因型.在此基础上,可利用单倍体种子胚乳或二倍体叶片等材料实现对银杏无性系品种的有效分子鉴别,为银杏的良种鉴别、保护和登录提供了科学依据. 相似文献
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用RAPD技术估测柳树种及无性系的变异 总被引：12，自引：2，他引：12
利用１２个引物对柳树２种１６个无性系基因组分别进行了ＲＡＰＤ检测，从电泳图谱可直观看出，引物ＣＨＬ－１可对柳树两种识别：引物Ｄｅｃａ－４可对１６个无性系基因组扩增产物经电泳产生的图谱中ＤＮＡ条的统计，利用ＮＴ－ＳＹＳ对其进行分析建立系统树，结果表明，利用ＲＡＰＤ获得的两种柳树种的分类结果与经典方法得到的结果一致。 相似文献
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马尾松毛虫质型多角体病毒单克隆抗体的制备和应用 总被引：1，自引：0，他引：1
纯化的马尾松毛虫质型多角体病毒（ＤｐＣＰＶ）云南文山株的病毒粒子经ＳＤＳＰＡＧＥ分析,其结构蛋白有１２０ｋｄ、１１６ｋｄ、１１０ｋｄ、６６ｋｄ和３３ｋｄ５个组分,而它的多角体蛋白含３０ｋｄ和２８ｋｄ两个主要组分。以纯化的ＤｐＣＰＶ粒子为抗原制备了２Ｄ５、２Ｄ１０、３Ｄ４和６Ｂ３共４种单克隆抗体,并测定了它们的亚类。制得的单克隆抗体用于ＤｐＣＰＶ的ＥＬＩＳＡ检测。对ＤｐＣＰＶ在棉铃虫卵巢细胞系ＳＦＥＨＡ８２１２和ＳＦＥＨＡ８３１中增殖动态的检测结果表明,ＤｐＣＰＶ感染培养细胞具有释放病毒量小和呈现持续感染等特点。用Ｗｅｓｔｅｒｎ印迹法在ＳＦＥＨＡ８３１细胞感染ＤｐＣＰＶ后第１８ｈ检测到病毒抗原的合成。运用所建立的免疫学检测方法,对几批采自ＤｐＣＰＶ防治林区的幼虫样品进行分析,结果表明,该方法适用于对ＤｐＣＰＶ的防治效果及其自然流行进行长时期、大规模的监测。 相似文献
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沙拐枣同化枝的光合作用和呼吸作用对生长季节,光照强度高温和干旱的响应 总被引：10，自引：2，他引：8
沙拐枣同化枝的Ｐｎ随光照强度增加递增并具有高的ＬＳＰ。夏季的Ｐｎ、Ｐｔ和ＬＳＰ高于秋季，而ＬＣＰ、Ｒ和饱和光照下的ＣＯ２ＣＰ低于秋季。表明沙拐枣是阳地生态型植物，９月的同化枝已开始衰老。在饱和光照下，Ｐｎ随处理温度上升明显下降，相反Ｒ升高，但Ｐｔ在夏季和秋季均只有轻度变化。说明Ｐｎ下降是由Ｒ升高的致。在干旱影响下，同化枝的水势随干旱时间延长递降，Ｐｎ、Ｒ、Ｒｐ、Ｐｔ、ＬＳＰ、ＬＣＰ和Ｃｈｌ含量均呈现同样变化，饱和光照下的ＣＯ２ＣＰ则明显升高，在１２天干旱后水势下降至－１．８６ＭＰａ时，Ｐｔ仍能保持５８．５％，将干旱和高温结合处理沙拐枣柏株，结果与干旱处理相似，但受等程度加重，Ｐｔ仍能保持对照的４１．５％。这些结果表明，沙拐枣除形态结构适应外，生理功能对强光、高温和干旱的荒漠环境也有广泛的适应能力。 相似文献
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四个杨树无性系在不同温度和相对湿度条件下净光合速率的比较研究 总被引：9，自引：4，他引：9
选择两个速生的（群众杨－４４和欧美杨２５／８６）和两个慢生的（小叶杨和欧美杨２８／８６）杨树无性系，在水培和生长箱控制条件下，测定了净光合速率（Ｐｎ）、光饱和点（ＬＳＰ）、光补偿点（ＬＣＰ）、表观光量子产量（Φ）、气孔传导度（Ｃｓ）和蒸腾速率的变化特点。结果表明，四个杨树无性系的ＬＳＰＬＣＰ，Φ．ＣＳ和最大的Ｐｎ对温度和相对湿度的响应特征不同。这可能反映了杨树无性系生态型差别，同时也为杨树无性系在生理指标基础上进行早期选择提供了证据 相似文献
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Two anonymous DNA markers that are revealed by single‐strand conformational polymorphism (SSCP) analysis were developed for detection of polymorphisms in Melampsora medusae f. sp. deltoidae (Mmd). Mono‐uredinial isolates of Mmd were first obtained, DNA was extracted from urediniospores and random amplified polymorphic DNA (RAPD) products of eight mono‐uredinial isolates were separated on a SSCP gel to identify differences among them. Bands representing putative polymorphic loci among the eight isolates tested were excised from the SSCP gel and re‐amplified by polymerase chain reaction (PCR), and then cloned and sequenced. A primer pair was designed to amplify a DNA fragment of a size suitable for SSCP analysis (<600 bp) for two out of three DNA fragments sequenced. Each set of primers amplified a PCR product for all eight isolates that were initially used to generate them and the resulting PCR products were analysed by SSCP. Polymorphisms among isolates were identified for both putative loci. The two primer pairs amplified a PCR product of the expected size on an additional 32 mono‐uredinial isolates of Mmd tested. From the overall 40 mono‐uredinial isolates tested, 5 and 11 alleles were detected, and 12 and 34 isolates showed to be heterozygous, as indicated by the presence of more than two bands on the SSCP gel, at loci A and B, respectively. The primer pairs were tested for specificity against 106 fungal isolates belonging to various taxa, including other rusts, and against DNA extracted from greenhouse‐grown healthy poplar leaves. DNA amplification products of the expected size were obtained only when Mmd DNA was present. Optimization of PCR conditions with these two primer pairs allowed genotyping directly from single uredinia extracted from infected leaves, thus alleviating the need to culture the fungus to characterize individuals, hence making it possible to process large numbers of samples for population studies. 相似文献
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Unlike maternal inheritance in sexual hybridization, plant somatic hybridization allows transfer, mixing and recombination of cytoplasmic genomes. In addition to the use of somatic hybridization in plant breeding programs, application of this unique tool should lead to a better understanding of the roles played by the chloroplastic and mitochondrial genomes in determining agronomically important traits. The nucleotide sequences of cytoplasmic genomes are much more conserved than those of nuclear genomes. Cytoplasmic DNA composition in somatic hybrids is commonly elucidated either by length polymorphism analysis of restricted genome regions amplified with universal primers (PCR-RF) or by hybridization of total DNA using universal cytoplasmic probes. In this study, we demonstrate that single-stranded conformational polymorphism (SSCP) analysis is a powerful, quick and easy alternative method for cytoplasmic DNA characterization of somatic hybrids, especially for mitochondrial DNA. The technique allows detection of polymorphisms based on both size and sequence of amplified targets. Twenty-two species of the subfamily Aurantioideae were analyzed with eight universal primers (four from chloroplastic and four from mitochondrial regions). Differences in chloroplastic DNA composition were scored in 98% of all possible two-parent combinations, and different mitochondrial DNA profiles were found in 87% of them. Analysis by SSCP was also successfully used to characterize somatic hybrids and cybrids obtained by fusion of Citrus sinensis (L.) Osb. and C. excelsa Wester protoplasts. 相似文献
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运用改良SDS(十二烷基硫酸钠)法,从马尾松、黄山松、黑松等松树种子胚乳中提取到较高纯度的DNA,并对其纯度、浓度及产率进行了分析.经RAPD检测表明,DNA扩增效果良好,完全能满足常规DNA实验的要求,为针叶树种等其它小粒种子DNA的提取提供了经济、快速、可靠的实验方法. 相似文献
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A reliable,efficient anther culture system,the dominant technique for generating haploid plants in breeding programs,that can be used for generating transgenic poplar plants has been needed.In the present study,therefore,an anther culture system was developed using isolated mid-and late-uninucleate anthers of poplar(Populus simonii x P.nigra).From a combination of SSR and ploidy analyses,six double haploid and two haploid lines were characterized from 86 plants grown from 16 regenerated anther cultured lines.After 48 months of development,two plant lines from the regenerated plants maintained their haploid level in vitro for over 2 years.A number of haploid plants from the different lines weretransferred to soil.The leaves of these transplants were then used as explants for transformation with the APETALA1(AP1) gene using Agrobacterium tumefaciens.Overexpression of AP1 in haploid poplar induced early flowering with obvious petals when ectopically expressed.To our knowledge,this is the first report on changes in flowering time in AP1-trangenic poplar,which is important for elucidating the regulatory mechanism of tree flower development. 相似文献
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以华山松（Pinus armandi）种子胚乳为实验材料,分别采取经典CTAB法、简易CTAB法和SDS法微量法提取华山松基因组DNA,并通过琼脂糖凝胶电泳、紫外分光光度计分析、标准差和标准误、卡方检验和SPSS软件显著性分析,将它们在DNA产量、质量和方法稳定性等方面的优缺点进行总结。得出结论：通过对数据进行卡方检验,所有数据都是差异是由方法所引起的,不是偶然误差所引起的;相比于经典CTAB法,SDS微量法和简易CTAB法的（OD260-OD320）/（OD280-OD320）标准差和标准误相差不大,数据相对精确,结果相对精确;通过SPSS软件分析分析,简易CTAB法和其他2种方法相比存在显著差异性,SDS微量法与经典CTAB法的P〉0.05,差异不显著,提取效果相差不大。综上所述：相比于简易CTAB法,SDS微量法与经典CTAB法的（OD260-OD320）/（OD280-OD320）都接近2.0,可能存在RNA污染污染,DNA的浓度也相对较低,SDS微量法与经典CTAB法不适合华山松胚乳DNA提取。简易CTAB法的（OD260-OD320）/（OD280-OD320）比率很接近1.80,不存在蛋白质和RNA污染,DNA纯度也最好,DNA浓度最高,比较适合华山松胚乳DNA提取。 相似文献
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Pollen dispersal was estimated in two test plots in a hinoki (Chamaecyparis obtusa) seed orchard using a chloroplast DNA marker, the spacer region between thetrnD andtrnY genes, and SSCP (single strand conformation polymorphism). In Plot 1, 2,020 seeds from 40 trees within 30 m of the marker
tree were analyzed using the PCR-SSCP method. In Plot 2, 1,850 seeds from 37 trees were analyzed in the same manner. The results
revealed that the maximum pollen dispersal distance in the two plots exceeded 25 m. Pollen dispersal appeared to be inversely
proportional to the distance from the marker tree. The effective pollen dispersal was suggested to be less than about 20 m
in a mature hinoki seed orchard. Adjacent trees had an excessive influence when the pollen density was increased by artificial
flower stimulation. Therefore, it was suggested that seed production better resembles ideal random mating when carried out
as naturally as possible. In conclusion, the SSCP chloroplast DNA marker was a useful tool for amassing basic information
on pollen management in seed orchards of coniferous species. 相似文献