共查询到19条相似文献，搜索用时 328 毫秒
1.
不同条件对绒山羊体外培养初级毛囊生长的影响(英文) 总被引：1，自引：1，他引：0
[Objective] The aim of this study is to lay a foundation for illustrating the biological characteristics and growth regulation mechanism of hair follicles.[Method]Cashmere goat primary hair follicles were separated under aseptic condition and cultured in serum-free DMEM and serum-free Williams E media respectively;subsequently,the growth rate and morphological changes were observed under the inverted microscope.[Result]Hair follicles cultured in serum-free DMEM media showed a growth rate of 0.034 mm/d during the first 3 days,whose structure and morphological characteristics could maintian a stable status for a long time in the growth process.Hair follicles grew much faster in the serum-free Williams E media with a growth rate of 0.077 mm/d during the first 3 days.[Conclusion]There were significant differences(P<0.05)between the growth of cashmere goat hair follicles cultured in the 2 kinds of media.Serum-free Williams E medium was superior to serum-free DMEM medium. 相似文献
2.
Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray 
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下载免费PDF全文 DI Jiang XU Xin-ming Lazate Ainiwaer ZHANG Yan-hua TIAN Ke-chuan YU Li-juan WU Wei-wei Hanikezi Tulafu FU Xue-feng Marzeya Yasen 《农业科学学报》2014,13(10):2243-2252
In order to study the molecular mechanism involved in cashmere regeneration, this study investigated the gene expression profile of skin tissue at various stages of the cashmere growth cycle and screen differentially expressed genes at proangen in 10 cashmere goats at 2 years of age using agilent sheep oligo microarray. Significance analysis of microarray （SAM） methods was used to identify the differentially expressed genes, Hierarchical clustering was performed to clarify these genes in association with different cashmere growth stages, and GO （Gene ontology） and the pathway analyses were con-ducted by a free web-based Molecular Annotation System3.0 （MAS 3.0）. Approximately 10200 probe sets were detected in skin tissue of 2-yr-old cashmere goat. After SAM analysis of the microarray data, totally 417 genes were shown to be differentially expressed at different cashmere growth stages, and 24 genes are significantly up-regulated （21） or down-regulated （3） at proangen concurrently compared to angen and telogen. Hierarchical clustering analysis clearly distinguished the differentially expressed genes of each stage. GO analysis indicated that these altered genes at proangen were predominantly involved in collagen fibril organization, integrin-mediated signaling pathway, cell-matrix adhesion, cell adhesion, transforming growth factor-β （TGF-β） receptor signaling pathway, regulation of cell growth. Kyoto encyclopedia of genes and genomes （KEGG） analysis showed that the significant pathways involved mainly included focal adhesion and extracellular matrixc （ECM）-receptor interaction. Some important genes involved in these biological processes, such as COL1A1, COL1A2, COL3A1, SPARC, CYR61 and CTGF, were related to tissue remolding and repairing and detected by more than one probe with similar expression trends at different stages of cashmere growth cycle. The different expression of these genes may contribute to understanding the molecular mechanism of cashmere regeneration. 相似文献
3.
绒山羊瘤胃甲烷菌mcrA基因的RFLP分析(英文) 总被引：3，自引：0，他引：3
[Objective] The aim of this study is to understand the population composition of methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. [Method] Total DNAs of various bacteria in rumen fluid were isolated for PCR amplification using the specifically designed primers based on conservative mcrA sequence of methanogens; then mcrA specific clone library was accordingly established. The restriction fragment length polymorphism(RFLP) of the library was further analyzed by digestion of restriction enzyme Taq I. [Result] One hundred and five randomly selected specific colonies were classified into six RFLP types, among which the dominant type accounts for 38%, and other types account for 27%, 18%, 5.5% and 4.5%, respectively. [Conclusion] There are at least six different methanogens in rumen fluid of grazing Inner Mongolian cashmere goat. 相似文献
4.
不同肥料对椭圆叶花锚生长发育的比较研究(英文) 总被引：7，自引：1，他引：6
[Objective] The aim was to study the effect of different treatments on development and growth of Halenia ellipitica D.Don.[Method] Three treatments (urea treatment group, diammonium phosphate treatment group and control group) were set in the experiment with 3 replications to detect plant height,root length, leaf number, branch number, flower number and biomass of Halenia ellipitica D.Don. in all experimental groups.[Result] The leaf number,branch number, flower number and output of crude drug of Halenia ellipitica D.Don. in urea treatment group were more than that of Halenia ellipitica D.Don. in diammonium phosphate treatment group and control group,however,the plant height and root length were not significantly different among all groups.[Conclusion] The urea generated better effect on Halenia ellipitica D.Don. cultivation than that produced by diammonium phosphate treatment. 相似文献
5.
[Objective] The countermeasure on the number of fructification of Spartina alterniflora in the period of sexual reproduction and the component of seed yielding construction was explored. [Method] The Spartina alterniflora in Mangroves conservation zone located at Hepu of Guangxi being taken as experimental material, its morphological and quantitative characteristics, as well as the weight of 100 full seeds at maturity stage in three different growth conditions(clay, loam and sand) were studied. [Results] The results showed that Spartina alterniflora had the best growth pattern in the loam. The morphological factors of fructification of S.alterniflora grown in sand were larger than in others. In the three growth conditions the order of quantitative characteristics of fructification of S.alterniflora was clay > sand > loam and the seeds in spikelet at top position were more maturity than those at the button position. [Conclusion] In good condition, the Spartina alterniflora growth was vigor but the ratio of seed-setting was low. 相似文献
6.
不同药剂处理对受寒害木薯出苗生长的影响(英文) 总被引：6，自引：1，他引：5
The rooting and growth of frozen cassava under different chemical treatments were studied.The result has demonstrated that disinfection effect could increase rooting rate and seedling emergence rate of cassava seed stem and decrease the rate of mildew.The sportak treatment could generate better effect(rooting rate and seedling emergence rate were 63%,the mildew rate of stem was 28%).Among different rooting reagents,Genwang+lime treatment generated better effect on rooting rate and emergence rate of frozen cassava than these of other groups and control group.It was concluded from the effects of different rooting reagents on growth of seed stem that Genwang+lime treatment could promote elongation and growth of cassava significantly(the mean plant height of experimental groups inceased 8.58 cm compared with that of control group) while paclobutrazol+lime generated the best effect on crassation of stem(the stem diameter of experimental group increased 0.4 cm compared with that of control group). 相似文献
7.
《农业科学与技术》2013,(10):1439-1442
[Objective] The aim was to explore the effects of different factors on rapid propagation and seedling survival of Lonicera japonica Thunb. [Method] The cuttings of 1, 2 or 3 years old, with different length, were soaked in different hormones at different concentrations for different time and planted at different dates. Finally, their rooting rate and survival rate in different treatments were measured and compared. [Result] The rooting rate and survival rate of one-year old cuttings with length of 10-13 cm and planted on September 20 and October 20, 2012 were relatively higher among all the treatments. Hormone treatment could significantly promote the callus formation and accelerate the rooting of the cuttings, and improve their survival rate. The cuttings treated with 120-200 mg/L ABT1 or NAA for 35-45 min had higher rooting rate and survival rate. Among them, the survival rate of cuttings treated with 160 mg/L ABT1 for 35 min was the highest, up to 92.5%. [Conclusion] The study provided basis for improving the survival rate of L. japonica by cuttage, and for optimizing the rapid propagation technique. 相似文献
8.
LIU Zhi-da XING Wan-jin WANG Lian-qing LV Li-xia 《中国农业科学(英文版)》2010,9(4):605-613
Izumol is a novel member of the immunoglobulin superfamily locating on sperm, and is indispensable for sperm-egg fusion. According to its immunoglobulin-like domain in the extracellular region, Izumol was fractionated into 6 fragments （F0-F5） which were ligated with pGEX-4T1 to construct the prokaryotic expression vectors pGEX-Fn. The recombinant plasmids were transformed into Escherichia coli BL21 （DE3） and the GST-Fn fusion proteins were expressed successfully by induction with IPTG. GST-F0, a recombinant fusion protein of GST with the full length of extracellular region of mature cashmere goat Izumol, was purified by polyacrylamide gel slicing method and was used as an antigen to immunize the Kunming mouse to generate anti-GST-Izumol ascetic polyclonal antibody with intraperitoneal injection of S 180 cells. Subsequently, the anti-GST-Izumol polyclonal antibody was purified with miscellaneous antigen by glutaraldehyde cross-linking method. Western blotting analysis showed that the purified ascetic polyclonal antibody had high affinity to all 6 GST-Izumol fragment fusion proteins. Immunohistochemical analysis with this antibody displayed that the cashmere goat Izumol proteins were at the equatorial segment of sperm head surface. These results indicate that this polyclonal antibody has high specificity and lays the foundations for further study on the expression pattern of Izumol in cashmere goat testis and binding abilities of each extra-membrane fragment of Izumol to the egg surface. 相似文献
9.
番茄内生细菌的分离及其促生活性研究(英文) 总被引：5，自引：1，他引：4
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity. 相似文献
10.
番茄内生细菌的分离及其促生活性研究(英文) 总被引：4，自引：0，他引：4
[Objective] The study aimed to investigate the growth-promoting activities of endophytic bacteria from tomato plants.[Method]The endophytic bacteria isolated from different tissues of tomato plants were analyzed for the effects of their growth-promoting activities on the germination and growth of tomato plants.The bacteria with growth-promoting activity were preliminarily identified.[Result]Totally 59 endophytic bacterial strains were isolated from roots and stems of tomatoes,of which 4 showed significantly growth-promoting activity to germination and growth of tomato.The results suggest that these strains are endowed with the potential capability of growth-promoting.[Conclusion]The endophytic bacteria with growth-promoting activity were found among the isolates from tomato plants.This provided a good foundation for utilization of these bacteria with growth-promoting activity. 相似文献
11.
雌二醇对体外培养绒山羊初级毛囊的影响 总被引：2，自引：0，他引：2
[目的]初步探讨雌二醇对绒山羊初级毛囊形态及生长的影响。[方法]在Williams E无血清培养基中分别添加不同剂量的17-βE2（0.1、1.0、10.01、00.0 nmol/L）,观测毛囊体外培养过程中生长速度及形态结构的变化。[结果]0.1 nmol/L 17-βE2组与对照组生长速度基本相当,而1.0、10.0和100.0 nmol/L 17-βE2对毛囊的生长均有不同程度的抑制;毛囊形态也表现出相应的变化。[结论]该研究为探明雌激素对绒山羊毛囊生长的调控机制奠定了一定的基础。 相似文献
12.
[目的]为阐述毛囊生物学特性和生长调控机制奠定基础。[方法]在无菌条件下分离绒山羊初级毛囊并培养于无血清DMEM培养基和Williams E培养基中,观测毛囊的生长速度及形态学变化。[结果]毛囊在无血清DMEM培养基中的生长速度是0.034 mm/d(前3d),在生长过程中毛囊的层次结构及形态可长时间保持不变;在无血清Williams E培养基中生长速度是0.077 mm/d(前3 d),明显加快。[结论]绒山羊初级毛囊在2种培养基中生长速度差异显著(P<0.05),更适合在Williams E无血清培养基中生长。 相似文献
13.
内蒙古阿尔巴斯绒山羊毛囊结构及形态发生过程研究 总被引：5，自引：4，他引：5
【目的】了解绒山羊毛囊的组织结构及毛囊形态发生变化过程，为研究绒山羊绒毛发育的分子调控机理奠定组织学基础。【方法】制作内蒙古阿尔巴斯绒山羊胚胎皮肤组织切片，显微镜下观察照相。【结果】绒山羊的毛囊结构同其它动物一样，由毛球、连接组织鞘、外根鞘、内根鞘和毛干几部分组成。在胎龄55～65 d毛囊开始发生形成毛芽；胎龄135 d，大多数初级毛囊和一部分次级毛囊发育基本成熟；次级毛囊是初级毛囊的一个分支。【结论】了解了绒山羊毛囊的结构组成及毛囊从发生到发育成熟的整个形态变化过程，为相关领域研究者提供借鉴。 相似文献
14.
[目的]探索新疆山羊毛绒性状和皮肤毛囊的发育规律,[方法]分别在3月、6月、9月、12月取毛样和体侧部皮肤,检测毛样绒细度、长度、净绒率。皮肤切片后采用sacpic法染色,观察毛囊的生长发育规律。[结果]新疆山羊绒平均细度约为15.16μm,长度为46.6mm,净绒率为28.8%;毛囊活性、深度、密度均以9月份为最高,3月份最低,说明9月为新疆山羊毛囊的兴盛期,3月份为静止期。[结论]从绒细度、长度来看,新疆山羊属于绒山羊中的优秀品种,而且初、次级毛囊密度高,在后期选育对于提高生产性能具有很大的潜力。 相似文献
15.
为分析绒山羊皮肤中受Hippo信号通路调控的绒生长候选基因集,本研究选取6只罕山白绒山羊随机分为对照组和褪黑素处理组,每组设3个重复。以自然年为试验周期,每月采集皮肤样本进行RNA测序,使用Bowtie、TopHat、Cufflinks、Cuffmerge、Cuffdiff、Cuffcompare、CPAT和CPC软件分析DE-mRNA和DE-lncRNA,联合PCA分析它们对次级毛囊生长周期的应答,利用WGCNA挖掘调控山羊绒生长的基因模块,GSEA分析候选基因集的生物学功能。结果表明：1)筛选得到组间DE-mRNA 2 024个和DE-lncRNA 329个,它们应答了绒山羊皮肤次级毛囊的生长周期。2)获得有生物学意义的对照组7个和试验组9个基因模块。3)深入分析富集到平衡哺乳动物皮肤生长分化、毛囊形态发生的Hippo信号通路基因模块,揭示了外源褪黑素诱导下调控山羊绒生长候选基因集191个mRNA和49个lncRNA的共表达调控网络关系。4)发现候选基因集与皮肤发育生物学过程(|NES|>1且FDR<25%)、Hippo信号通路(|NES|>1且FDR<25... 相似文献
16.
为分析chi-miR-107-3p及相关基因在不同品系绒山羊皮肤毛囊生长发育周期中的表达情况,本研究分别在毛囊休止期(4月)、兴盛前期(7月)、兴盛期(9月)和退行期(1月)采集了阿拉善型绒山羊(季节性长绒)和敏盖绒山羊(常年长绒)体侧皮肤组织,通过组织切片比较分析组织形态,实时荧光定量PCR法检测chi-miR-107-3p及ADCK5、DSBC1、ARSA、FMAP4、RHBDF2和ALDH3A2等6个相关基因表达量。结果表明:1)两种品系的绒山羊毛囊在形态特征上基本一致,但在同一时期生长规律不同;2)chi-miR-107-3p和RHBDF2基因在两种品系绒山羊皮肤毛囊不同发育周期的表达量呈相反趋势。在次级毛囊重建初期,chi-miR-107-3p表达量较高,而RHBDF2基因的表达量极低,随着次级毛囊重建逐步完成,chi-miR-107-3p表达量显著降低,RHBDF2基因的表达量逐渐增高。进入退行期,chi-miR-107-3p的表达量逐渐增高,而RHBDF2基因的表达量逐渐降低,休止期,chi-miR-107-3p的表达量达到最大值,此时RHBDF2基因的表达量最小,推测chi-miR-107-3p可能负向调控RHBDF2基因的表达;3)相关性分析表明,chi-miR-107-3p和RHBDF2基因表达水平均与皮肤毛囊性状显著相关(P<0.05),说明chi-miR-107-3p和RHBDF2基因均为两品系绒山羊皮肤毛囊生长发育的重要影响因子;4)ALDH3A2基因的表达量在皮肤毛囊休止期至兴盛期的过程中逐渐降低,由退行期至休止期的过程中逐渐增高。ALDH3A2基因与皮肤毛囊各性状均显著相关(P<0.05),表明高表达的ALDH3A2可能抑制两品系绒山羊毛囊的生长发育,是调控皮肤毛囊生长发育的重要影响因子,其他基因对绒山羊皮肤组织生长均无影响。本研究证实羊绒生长发育过程离不开miRNA及其相关基因参与调控,为进一步研究毛囊发育分子机理提供了参考,为实际生产中进行羊绒增产提供理论依据。 相似文献
17.
褪黑激素对内蒙古绒山羊初级毛囊体外培养影响的研究 总被引：1，自引：0，他引：1
为探究褪黑激素(MT)对绒山羊毛囊体外生长及毛囊形态的影响,采用物理切割法结合胶原酶消化分离绒山羊初级毛囊,并通过设置酶的不同体积分数(0.10%、0.20%、0.25%、0.30%、0.35%、0.40%和0.45%)以及相应的消化时间(100、80、70、60、50、45和35min)确定型胶原酶消化的最佳条件。在初级毛囊体外培养的基础上,添加褪黑激素(300pg/mL)连续培养5d,在显微镜下观察毛囊每天的生长情况。结果显示,毛囊分离过程中胶原酶消化的最佳体积分数和时间为0.30%、60 min,褪黑激素对初级毛囊体外生长具有极显著的(P0.01)促进作用。 相似文献
18.
19.
【目的】建立三褶虾脊兰（Calanthe triplieata）无菌播种快繁技术体系。【方法】以三褶虾脊兰种子为外植体,采用种子→原球茎→完整植株途径,探讨不同组合（0．5mg／L6-BA、0．1mg／LNAA和1．0g／LAC）、附加物[椰汁（CM）、土豆泥、香蕉泥]及基本培养基（花宝1号、1／2花宝1号、MS和1／2MS）对三褶虾脊兰种子萌发、原球茎诱导、增殖、分化、生根的效果,筛选适宜的培养基。【结果】成熟种子在1／2花宝1号＋0．5mg／L 6-BA＋0．1mg／L NAA＋200ml/L CM＋1．0g／L AC＋20．0g／L蔗糖培养基中培养150d左右可形成原球茎,萌发率超过80％以上;诱导原球茎在1／2MS＋100mL／LCM＋2．0mg／L6-BA＋0．1mg／LNAA＋1．0g／LAC＋20．0g／L蔗糖培养基上的增殖效果最好,增殖率为4．5;原球茎接种于花宝1号＋0．1mg／L6-BA＋0．1mg／LNAA＋100．0g／L土豆泥＋1．0g／LAC＋20．0g／L蔗糖培养基上分化培养40d后,再转入花宝1号＋0．1mg／LNAA＋100．0g／L土豆泥＋2．0g／LAC＋20．0g／L蔗糖培养基上培养40d,可诱导形成完整植株。在花宝1号培养基中添加NAA和土豆泥或香蕉泥,原球茎生根率均达100．0％,但土豆泥的成本最低。【结论】建立了比较完善的王褶虾脊兰无菌播种和快繁技术体系,为其种质资源保护、种苗繁育以及产业开发提供技术支持。 相似文献