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1.
Soybean meal (SBM) treated with 70% ethanol at 80 C (ET), nontreated SBM (NT) or a ureacasein-corn mix (UC) was fed to steers fitted with ruminal and duodenal cannulae to study ruminal N metabolism. Sodium chloride (NaCl) was ruminally infused at 0 or 500 g/d. Nitrogen supplements provided approximately 70% of total dietary N. Experimental design was a 6 X 6 Latin square with a 3 X 2 factorial arrangement of treatments. Total duodenal N flows and non-ammonia, non-bacterial-N (NANB-N) flows were higher (P less than .05) when steers were fed SBM treatments compared with UC, and higher (P less than .05) when steers were fed ET compared with NT. Percentage of SBM-N escaping ruminal degradation was greater (P less than .05) when steers were fed ET compared with NT, and greater (P less than .05) when NaCl was infused into the rumen. Duodenal flows of total, indispensible and dispensible amino acids were increased (P less than .05) when steers were fed SBM treatments compared with UC, and greater (P less than .05) when steers were fed ET compared with NT. No differences in soluble N flows at the omasum were observed due to treatment. Bacterial protein comprised the majority of the N leaving the rumen. Both ruminal NaCl infusion and ethanol and heat treatment of SBM increased ruminal SBM-N escape.  相似文献   

2.
Seven Holstein steers (340 kg) fitted with ruminal, duodenal and ileal cannulae were used to measure the influence of supplemental N source on digestion of dietary crude protein (CP) and on ruminal rates of protein degradation. Diets used were corn-based (isonitrogenous, 12% CP on a dry matter basis, and isocaloric, 80% total digestible nutrients) with urea, soybean meal (SBM), linseed meal (LSM) or corn gluten meal (CGM) as supplemental N. Ruminal ammonia N concentrations were higher (P less than .05) in steers fed LSM than in those fed CGM, but did not differ from those in steers fed urea or SBM (11.7, 6.7, 9.1 and 9.2 mg/100 ml, respectively). Due to the high degradability of urea, ruminal digestion of dietary CP was greater (P less than .05) in steers fed urea than in those fed CGM, but intermediate in steers fed SBM and LSM (58.4, 48.8, 53.1 and 53.9%, respectively). Flow of bacterial nonammonia N to the duodenum was highest (P less than .05) in steers fed SBM or LSM, intermediate (P less than .05) for urea and lowest (P less than .05) for CGM (86.8, 86.1, 76.3 and 65.9 g/d, respectively). Efficiency of bacterial protein synthesis was lowest in steers fed CGM and differed (P less than .05) from SBM (15.6 vs 21.8 g N/kg organic matter truly digested, respectively). Rate of ruminal digestion for SBM-CP differed (P less than .05) from that of CGM-CP but not from that of LSM-CP (17.70, 5.20 and 10.13%/h, respectively). The slow rate of ruminal degradability of CGM resulted in increased amounts of dietary protein reaching the intestinal tract but lower amounts of bacterial protein, thus intestinal protein supply was not appreciably altered.  相似文献   

3.
Two experiments were conducted to determine the effects of supplemental CP source and level of urea on intestinal amino acid (AA) supply and feedlot performance of lambs fed diets based on alkaline hydrogen peroxide-treated wheat straw (AHPWS). In Exp. 1, five cannulated (ruminal, duodenal, and ileal) crossbred wethers (61 kg) were used in a 5 x 5 Latin square design. Treatments consisted of different sources of CP and included soybean meal (SBM), a combination of urea, distillers dried grains (DDG), and fish meal, each provided an equal portion of supplemental CP (UDF), and three levels of urea (17, 33, and 50% of supplemental CP) fed in combination with DDG (U17, U33, and U50). Organic matter and N digestibilities decreased (P less than .05) when lambs were fed U17 compared with those fed SBM. There were no differences (P greater than .05) in bacterial N or AA flows to the duodenum due to CP source despite large differences in ruminal NH3 N concentrations and lower ruminal OM digestion when lambs were fed U17. Duodenal nonbacterial N and AA flows were highest (P less than .05) in lambs fed U17 and UDF and lowest when lambs were fed U50 and SBM. Lysine concentration in duodenal digesta decreased with incremental increases in DDG. In Exp. 2, 30 individually penned ram lambs (33 kg) were allotted to five CP treatments in a randomized complete block design. Treatments were similar to those of Exp. 1, with the exception that U17 was replaced by a 14% CP diet with SBM as the supplemental CP source; all other diets were formulated to contain 12% CP. Lambs fed U50 had decreased (P less than .08) ADG and gain/feed compared with all other treatments, and lambs fed UDF had greater (P less than .05) ADG and gain/feed than lambs fed U33. It was concluded that 17% of the supplemental CP from urea seems adequate to maximize bacterial protein synthesis and that no more than 33% of the supplemental CP should be provided by urea in diets based on AHPWS. Feeding a combination of ruminally resistant protein sources with complementary AA profiles of lysine and methionine (UDF) may enhance quality of protein entering the duodenum and feedlot performance.  相似文献   

4.
Two experiments were conducted to evaluate effects of supplemental protein source and ME intake (Exp. 1) on ewe nutritional status during pregnancy. Forty-two mature Targhee ewes were allotted randomly in Exp. 1 to one of six treatments (n = 7) using a 2 x 3 factorial arrangement. Ewes were individually fed chopped barley straw and supplements containing either urea (U), soybean meal (SBM), or blood meal (BM) plus SBM during the 84-d experiment. Straw and supplement intakes were adjusted to meet either 80 or 100% of ME requirements. In Exp. 2, 30 pregnant Targhee ewes that were allotted randomly to one of three groups (n = 10) were individually fed protein supplements similar to those in Exp. 1 and had group access to long-stem barley straw. In Exp. 1, ewes fed BM + SBM gained more (P less than .05) weight, lost less (P less than .05) body condition, and had increased (P less than .05) wool fiber length compared with ewes fed U; SBM ewes were intermediate (P less than .05) in response. Ewes fed BW + SBM had increased (P less than .05) blood urea N and albumin concentrations compared with ewes fed U. Metabolizable energy intake did not affect (P greater than .05) ewe performance or serum metabolite concentrations. Ewes fed BM + SBM in Exp. 2 gained more (P less than .05) weight than ewes fed either SBM or U, and ewes fed SBM lost less weight (P less than .05) than ewes fed U.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

5.
Two metabolism trials were conducted with yearling steers fed mature native forage to measure the effect of supplemental protein degradability on selected metabolic variables. Supplements contained 40% crude protein equivalence. In Trial 1, four abomasal-cannulated steers weighing 290 to 379 kg were fed supplements containing the following N sources: (1) 15% corn, 85% urea (U); (2) 100% soybean meal (SBM); (3) 10% corn, 40% soybean meal, 50% urea (SBM-U) and (4) 14% corn, 36% blood meal, 50% urea (BM-U). Equal portions of the daily diet (2.2% of body weight) were fed every 2 h. Treatment differences were not significant for organic matter digestibility, abomasal organic matter flow, nonammonia N flow, feed N flow, bacterial N flow and efficiency of microbial protein synthesis. There was a positive (P less than .05) relationship between quantity of slowly degraded protein fed and nonammonia N flow (r = .97) or feed N flow (r = .98). Escape N was determined to be 21.5, 16.5 and 54.2% in SBM, SBM-U and BM-U supplements, respectively. In the second trial, no supplement, SBM, SBM-U and BM-U were fed in a N balance trial. Dry matter, crude protein and neutral detergent fiber digestibilities were higher (P less than .05) for steers fed supplemented diets. Acid detergent fiber digestibility was higher (P less than .05) for steers supplemented with SBM than steers fed the unsupplemented diets. Nitrogen retention was greater (P less than .05) for cattle fed SBM and BM-U than for cattle fed SBM-U or no supplement.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

6.
Our objectives were to determine the influences of supplemental nonprotein N or protein on feed intake, digestibility, and postabsorptive N metabolism in sheep fed a high-concentrate diet for ad libitum consumption. Nine Romanov-sired, crossbred wethers (13 mo old; 52 kg) were fitted with catheters in a mesenteric artery, mesenteric vein, portal vein, and hepatic vein. Wethers consumed a 95% concentrate diet ad libitum. Treatments consisted of control (no supplemental N; 6.6% CP) or supplemental urea (11.4% CP), soybean meal (SBM; 11.2% CP) or ruminally undegradable protein (BFM; 11.2% CP; 50:50 blood meal and feather meal). Intake or apparently digested intake of DM, OM, and energy did not differ between control and N-supplemented (P > 0.40), or between urea- and protein-supplemented (P > 0.40), but were greater (P < 0.05) in SBM- than in BFM-supplemented wethers. Intake and apparently digested intake of N were less (P < 0.01) in wethers fed the control diet than in those receiving N supplementation but were less (P = 0.03) in BFM- than in SBM-supplemented wethers. Neither portal nor hepatic venous blood flows differed (P > 0.15) among treatments. Net portal release and hepatic uptake of alpha-amino N and ammonia N and hepatic release of urea N were greater (P < 0.05) in wethers supplemented with N than in controls, but portal-drained viscera (PDV) uptake of urea N did not differ (P > 0.40) among diets. Splanchnic release of a-amino N and ammonia N did not differ from 0 or among diets (P > 0.10), but net release of urea N was less (P = 0.05) for control than for sheep receiving N supplementation. No differences (P > 0.10) in blood concentration within vessel or net flux across PDV, hepatic, or splanchnic tissues of alpha-amino N, ammonia N, or urea N were observed among wethers receiving supplemental N. Net uptake of oxygen by the PDV did not differ among diets, but hepatic uptake was less (P < 0.05) in control and urea-supplemented sheep than in sheep receiving SBM or BFM. These observations suggest that the source of supplemental N had no large effects on the overall N economy of the animals used in this study.  相似文献   

7.
The intestinal supply of amino acids (AA) in sheep fed alkaline hydrogen peroxide-treated wheat straw (AHPWS)-based diets supplemented with soybean meal (SBM) or corn grain plus combinations of corn gluten meal (CGM) and blood meal (BM) was measured in a 5 X 5 latin square. Sheep (avg wt 45 kg) with ruminal, duodenal and ileal cannulas were fed diets containing 65% AHPWS supplemented with the following protein sources: soybean meal (SBM), corn gluten meal (CGM), blood meal (BM), 2/3 CGM:1/3 BM and 1/3 CGM:2/3 BM. Total nitrogen (N) flow at the duodenum was not affected (P greater than .05) by protein source. Flows of bacterial N and AA increased (P less than .05) and flows of nonbacterial N and AA decreased (P less than .05) when wethers were fed SBM vs corn plus other protein sources. When diets contained SBM, quantities of total AA at the duodenum were lower (P less than .05) and the profile of AA supplied to the intestine was altered substantially. Total flows of AA at the duodenum and total quantities of AA disappearing from the small intestine were similar (P greater than .05) for all diets containing BM and CGM, but flows and disappearance of valine, histidine, lysine and arginine increased linearly (P less than .05), whereas flows and disappearance of leucine, isoleucine and methionine decreased linearly (P less than .05) as BM replaced CGM in the diets. Results suggest that quantities of individual AA flowing to the duodenum and disappearing from the intestine of wethers fed AHPWS-based diets can be altered by source of dietary protein. Furthermore, feeding protein sources resistant to ruminal degradation in combination may improve the profile of AA supplied to the intestine.  相似文献   

8.
Eight multicatheterized wethers (35.9 +/- .8 kg BW) were used in a replicated 4 x 4 Latin square design to measure N retention and net uptake and release of plasma metabolites across the portal-drained viscera (PDV), hepatic (HEP), and total splanchnic (TS) tissues in response to changes in supplemental N source. Treatments selected to provide different amounts of undegradable intake protein (UIP) were urea, soybean meal (SBM), poultry by-product meal (PBM), and bloodmeal:corn gluten meal (BMCGM; 50:50 CP basis). Diets (urea, SBM, PBM, and BMCGM) contained 12.9, 13.8, 13.6, and 13.2% CP, respectively. Periods were 10 d, with total feces and urine collected on d 7 to 10 and blood sampled on d 10. Wethers were fed at 2% of BW in 12 daily portions. Nitrogen retention was 2.2, 3.3, 4.1, and 4.4 g/d for urea, SBM, PBM, and BMCGM, respectively. Urea had less (P < .01) N retention than SBM, PBM, and BMCGM; SBM had less N retention (P < .01) than PBM and BMCGM. Arterial, portal, and hepatic plasma flows were greater (P < .09) for SBM than for PBM and BMCGM (21 vs 16, 17; 84 vs 72, 72; 105 vs 87, 88 L/h). Portal plasma flow was greater (P < .10) for urea than for SBM, PBM, and BMCGM (85 vs 84, 72, 72 L/h). Portal-drained viscera and TS alpha-amino N (AAN) fluxes were less (P < .05) for PBM than for BMCGM (20.5 vs 26.6 and 7.2 vs 15.1 mmol/h), but TS AAN flux was less (P < .05) for urea than for SBM, PBM, and BMCGM (6.9 vs 16.9, 7.2, 15.1 mmol/h). Portal-drained viscera flux and HEP removal of NH3 N were greater (P < .001) for SBM than for PBM and BMCGM (27.7 vs 19.4, 20.6; -28.1 vs -20.0, -21.4 mmol/h). Gut use was less (P = .07) and HEP and TS fluxes of urea N were greater (P < .01) for SBM than for PBM and BMCGM (-4.92 vs -8.32, -7.93; 25.87 vs 16.54, 20.00; 20.95 vs 8.22, 12.07 mmol/h). These data suggest that PBM and BMCGM improved efficiency of N use compared with urea and SBM by reducing urinary N loss.  相似文献   

9.
Concurrent in vivo and in vitro studies were conducted to evaluate urea (U), soybean meal (SBM), ground soybeans (RAW), extruded soybeans (ES) or extruded soybeans plus urea (ES + U) as primary supplemental N sources in starter diets for Holstein steers. Three groups of 48 Holstein steers each were fed five different starter diets to 181 kg BW in three experimental periods over 2 yr. Average daily gains were similar (P greater than .05) for steers fed ES + U (1.12 kg), ES (1.08 kg) and SBM (1.09 kg) but lower (P less than .05) for those fed U (1.00 kg) or RAW (.97 kg) diets. Feed/gain was similar (P greater than .05) for ES-fed steers vs those fed other diets except U. From 181 to 477 kg, all steers were fed the same diet. Steers fed the RAW starter diet had the lowest (P less than .05) ADG for the entire period. The starter diets were used as substrates for ruminal microbial metabolism in eight dual-flow continuous culture fermenters. True OM digestion was higher and NDF and ADF digestion was lower (P less than .05) for the ES + U diet than for the ES diet. Dietary protein degradation was lowest (P less than .05) for the ES diet (64.4%). Total bacterial N flow was higher (P less than .05) with the ES + U, SBM and U diets than with the ES diet. Lysine flow was higher (P less than .05) for the ES + U diet than for all other diets except ES. Results of these experiments indicate that ES as a protected ruminal escape N source with or without added urea did not improve steer performance above that obtained from SBM in starter diets.  相似文献   

10.
Trials were conducted to evaluate effects of non-enzymatic browning of soybean meal (SBM) on efficiency of protein utilization and N digestibility. In trial 1, 48 Suffolk-Finnsheep lambs (22 kg) were fed 80 d to evaluate efficiency of protein utilization for growth when supplemental protein was fed as urea (U), commercial SBM (CS), or commercial SBM (pH 8.5, 83% dry matter) containing xylose (3 mol/mol SBM-lysine) and heated 30 min (XTS-30) or 55 min (XTS-55). Diets containing graded levels of N from CS, XTS-30 and XTS-55 were fed. Response criterion was efficiency of protein utilization, plotted as gains of lambs fed test proteins minus gain of lambs fed U vs supplemental test protein fed. Efficiencies of protein utilization were .62, 1.27 and .91 for CS, XTS-30 and XTS-55, respectively. Protein from XTS-30 was used more efficiently (P less than .05) than that from CS. In trial 2, apparent digestibility of N from CS (97%) was higher (P less than .01) than XTS-30 (77%) and XTS-55 (82%) by Suffolk-Finnsheep lambs (27 kg). In trial 3, 60 mixed-breed steers (218 kg) were fed individually for 105 d to evaluate glucose as a reducing sugar. Glucose-treated SBM (GTS) was prepared by mixing glucose (3 mol/mol SBM lysine) with SBM, adjusting pH and dry matter content to 8.5 and 80%, respectively, and heating at 150 C for 60 min. Supplemental N sources were U, CS, GTS and a 50:50 mixture (protein basis) of corn gluten meal and blood meal (CGM/BM).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

11.
Complementary responses between rendered protein meals were investigated in this study. In a preliminary trial using 12 mature wethers in two replications, there was no difference (P greater than .20) in N digestibility between meat and bone meal (MBM; 96.7%), feather meal (FTH; 89.8%), and soybean meal (SBM; 98.7%). In a 112-d growth trial, individually fed calves (n = 120; 230 kg) received graded levels of FTH, MBM, 50:50 MBM-FTH (CP basis), or SBM with or without tryptophan (Trp) supplementation. Additions of Trp increased plasma Trp levels (P less than .05) but failed to improve efficiency of protein utilization (P greater than .35). Pooled results showed that this efficiency was greater (P less than .05) for FTH (1.47) than for MBM (1.04), FTH:MBM (.80), or SBM (.66). A trial was conducted to determine whether Trp addition reduces growth response to FTH:MBM (50:50) combinations. Calves (n = 230; 285 kg) were blocked by sex and weight into six replications and received FTH:MBM supplying 35% of the supplemental CP fed alone or with a high or low level of Trp supplement. Negative (urea only) and positive controls were included. Calves receiving FTH:MBM combinations gained faster (P less than .10) and were more efficient (P less than .10) than urea-supplemented calves. Performance was not altered by Trp addition. Calves (n = 120; 230 kg) were individually fed in two replications (43 or 48% CP MBM in Replications 1 and 2, respectively) of a growth trial to determine whether there was a complementary response between blood meal (BM) and MBM.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

12.
Two gestating cow winter grazing trials and two lactating cow drylot trials were conducted to evaluate the use of a slowly degraded protein source in corn plant diets for mature beef cows. Gestating beef cows grazing cornstalks were supplemented with .86 kg/(cow.d) of a 20% crude protein equivalent (CPE) pellet (DM basis). In Trial 1 cows fed diets containing 80% dehydrated alfalfa meal (high DEHY) gained more weight (P less than .05) than those fed diets containing 40% dehydrated alfalfa meal (low DEHY) or urea but not more than the cows fed soybean meal (SBM); however, no differences among treatments were observed in Trial 2. Four lactation diets composed of ground corncobs and corn silage were supplemented with either urea, SBM, or two levels of dehydrated alfalfa meal (DEHY) as N sources. The same amount of supplemental N was fed in both trials, consisting of .31 kg of natural protein for the SBM and low DEHY treatments or .42 kg for high DEHY. Ammoniated corncobs replaced 35% of the ground corncobs in Trial 4. Diets were calculated to contain (DM basis) 55% TDN and 9% CPE in Trial 3 and 11% CPE in Trial 4. In Trial 3, lactating cows supplemented with DEHY gained more weight (P less than .10) than those fed the urea supplement but not more than those fed SBM. Gains by cows fed the urea- and SBM-supplemented diets were not different (P greater than .10). Cow weight gains in Trial 4 were not affected by type of protein supplementation.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

13.
Our objective was to determine the impact of supplemental energy, N, and protein on feed intake and N metabolism in sheep fed low-quality forage. Six Texel x Dorset wethers (16 mo, 63+/-3.1 kg) fitted with mesenteric, portal, and hepatic venous catheters were used in a Latin square design with five sampling periods. Lambs were fed chopped bromegrass hay (4.3% CP) to appetite, and a mineral mixture was given. Treatments were 1) control (no supplement), 2) energy (cornstarch, molasses, and soybean oil), 3) energy plus urea, 4) energy plus soybean meal (SBM), and 5) energy plus ruminally undegraded protein (RUP; 50:50 mixture of blood and feather meals). Supplements were fed once daily (.3% BW). Forage DMI did not differ (P = .13), but intake of total DM, N, and energy differed (P<.01) among treatments. Apparent digestibilities of DM, OM, and energy were less (P<.01) for control than for other treatments. Apparent N digestibility was least for control and energy and greatest for urea treatments (P<.05). As a result, digested DM, OM, and energy ranked from least to greatest were control, energy, urea, SBM, and RUP, respectively. Apparently digested N was 2.44, 2.24, 11.39, 9.80, and 11.25 g/d for control, energy, urea, SBM, and RUP (P<.01; SE = .10). Hour of sampling x treatment was a significant source of variation for blood concentrations of ammonia N and urea N, net ammonia N release from portal-drained viscera (PDV) and liver, and urea N release from splanchnic tissues. These results were primarily because patterns through time for the urea treatment differed from the other treatments. Net PDV release of alpha-amino N did not differ (P>.05) between control and energy treatments. Values for those treatments were about one-half of values for urea, SBM, and RUP treatments, which did not differ (P>.05). Hepatic net uptake (negative release) of alpha-amino N for control was 53% of values for the other treatments, which did not differ (P>.05). Net release of alpha-amino N from splanchnic tissues did not differ among treatments (P = .34) and did not differ from zero. The data indicate that arterial alpha-amino N concentration, hepatic alpha-amino N uptake, PDV release and hepatic uptake of ammonia N, and hepatic release of urea N were greater in energy than in control treatments. We also found that hepatic uptake of alpha-amino N was 187% of PDV release in energy-supplemented lambs. These results suggest that energy supplementation of a protein-limiting diet stimulated mobilization of body protein.  相似文献   

14.
Three experiments were conducted to determine the relative feeding value of frost-damaged soybeans (FDS) for ruminants. Frost-damaged soybean ether-extract content was variable and differed (P less than .05) from mature soybeans. Isonitrogenous supplementation of corn silage diets with soybean meal (SBM), SBM+soybean oil (SBO), mature raw soybeans (MSB), and FDS was compared in sheep. Acid detergent fiber and apparent N digestion were lower (P less than .001) for supplements containing oil. Nitrogen retention was reduced (P less than .07) only for raw soybean supplements. Ruminal NH3 N and branched-chain VFA concentrations differed (P less than .01) between SBM and supplements containing oil. Maximum tolerable inclusion level of FDS in corn silage diets was tested in wethers using diets containing 0, 7, 14, or 21% FDS. Dry matter and ADF digestibility declined linearly (P less than .01) with increasing dietary FDS. Ether extract digestibility was unchanged due to treatment, but GE digestibility decreased quadratically (P less than .01). The most pronounced decline in GE digestibility occurred when FDS increased from 14 to 21% of the diet. The effects of FDS on corn silage utilization were similar to MSB effects. Oil content and antinutritional factors contributed to detrimental effects. Frost-damaged soybeans should not exceed 14% of corn silage diets fed to growing wethers.  相似文献   

15.
Six beef steers (British x Brahman) cannulated at the rumen, duodenum and ileum (avg wt 334 kg) and three mature steers (British x British) cannulated at the esophagus were used in a replicated 3 x 3 latin square design and fed no supplement (C), .5 kg soybean meal (SBM) or .5 kg steam-flaked sorghum grain (SFS).head-1.d-1 (DM basis) while grazing blue grama rangeland. Periods of the latin square included a minimum of 14 d for adaptation and 11 d for esophageal masticate collection and digesta sampling. In September, October and November, respectively, forage collected by esophageally cannulated steers averaged 74.5, 88.8 and 71.0% grasses; 2.06, 1.53 and 1.77% N and 68.3, (P greater than .10) by treatment, but total N intake was greater (P less than .05) for SBM vs C and SFS treatments. No differences (P greater than .10) were detected among treatments in OM, NDF, ADF and N digestibilities in the rumen, small intestine or hindgut, but total tract OM digestibility was greater (P less than .10) for SBM and SFS than for C, and total tract N digestibility was greater (P less than .10) for SBM than for C or SFS. Duodenal ammonia N flow was greater (P less than .05) when SBM was fed that when SFS and C were fed, but microbial N and non-ammonia, non-microbial N flows and microbial efficiency were not altered by treatment. Likewise, ileal N flow was not affected (P greater than .10) by treatment. Particulate passage rate, gastrointestinal mean retention time, forage in vitro OM disappearance and in situ rate of forage NDF digestion also were not affected (P greater than .10) by treatments. Ruminal fluid volume was greater (P less than .05) for SFS vs SBM and C treatments, but no differences were noted in fluid dilution rate. Ruminal fluid ammonia concentration was greater (P less than .05) when SBM was fed than when SFS and C were fed (13.5, 9.9 and 8.7 mg/dl, respectively), whereas pH and total VFA concentrations were not different (P greater than .10). Proportion of acetate in ruminal fluid was less (P less than .10) for SBM and SFS than for C. Small amounts of supplemental SBM and SFS had little effect on forage intake, ruminal fermentation and site of digestion but both increased total tract OM digestion in steers grazing blue grama rangeland.  相似文献   

16.
Six steers (468 kg) with ruminal and duodenal cannulas were fed diets formulated for two levels of energy containing three crude protein (CP) sources in a 6 X 6 Latin square with a 2 X 3 factorial arrangement of treatments. Energy levels were 2.17 and 2.71 Mcal metabolizable energy (ME)/kg dry matter (DM) provided by hay-corn (H) and corn silage-corn (CS) diets, respectively. Soybean mean (SBM), corn gluten meal-urea (CGM) and urea (U) provided 33% of dietary CP in 12% CP diets. Apparent organic matter (OM) digested in the stomach was not affected (P greater than .05) by energy level or CP source, but OM truly digested in the stomach was greater (P less than .05) when steers were fed the CS compared with the H diet. Duodenal flow of non-NH3 N was greater (P less than .05) when steers were fed CS compared with H and when fed SBM or CGM compared with U. Efficiency of bacterial protein synthesis and duodenal bacterial N flow were increased (P less than .05) when steers were fed CS, but non-NH3, nonbacterial N flow to the duodenum was increased (P less than .05) when steers were fed H. When steers were fed CS rather than H, flows (g/d) of bacterial amino acids were greater (P less than .05), but flows of nonbacterial amino acids tended (P less than .08) to be less. Total amino acid flows were not affected (P greater than .05) by energy level. Duodenal flows of total amino acids tended (P less than .06) to be greater when steers were fed CGM compared with SBM or U, due mainly to an increased (P less than .05) flow of nonessential amino acids.  相似文献   

17.
Four rumen-cannulated, mature crossbred wethers were used in a 4 x 4 latin square experiment designed to examine the effects on protein metabolism of protein sources with various solubilities fed with low quality roughages. Four diets were used: (1) grass hay plus oat straw (control), (2) control plus urea, (3) control plus soybean oil meal (SBOM) and (4) control plus sunflower oil mean (SFOM). The diets were isocaloric, isofibrous and isonitrogenous (1.60% except for the control, which contained 1.09% N). The protein solubility means determined by three solvents (Burroughs' mineral mixture, McDougal's artificial saliva and sodium chloride) were 23.7, 50.5, 18.2 and 24.2% for the respective diets. The average protein solubilities of SBOM and SFOM were 20.5 and 35.4%, respectively. Apparent crude protein digestibilites were higher (P less than .05) for diets with higher protein solubility. The Urea diet (highest protein solubility) resulted in higher urinary N excretion (P less than .05) and lower N retention than the SBOM and SFOM diets. N retention was similar for the animals fed the SBOM and SFOM diets. Ruminal NH3-N and plasma urea N levels were higher (P less than .05) in animals fed the urea diet. The protein source altered VFA concentrations but not C2 to C3 ratios of VFA. The SBOM and SFOM produced more branched chain fatty acids. These data illustrate that protein sources of higher solubility result in higher crude protein digestion and higher N loss in the urine. N balance was similar with SFOM and SBOM. SFOM and SBOM can be used interchangeably on an equal protein basis for sheep fed high roughage diets.  相似文献   

18.
Hydrolyzed feather meal as a protein source for growing calves   总被引：1，自引：0，他引：1  
Growth, digestion and in situ studies were conducted to determine the protein value of hydrolyzed feather meal (Fth) for growing ruminants. Dacron bags containing blood meal (BM), Fth, corn gluten meal (CGM) and soybean meal (SBM) were suspended in the rumen of two steers for 12 h to estimate escape protein. The escape protein value for Fth, 69.1%, was less than that for BM (82.8%) and CGM (80.4%; P less than .05) but greater than that for SBM (26.6%; P less than .05). Apparent protein digestion by lambs was similar (P greater than .10) for isonitrogenous diets containing urea (U), BM, Fth, CGM and SBM. Amino acid contents of the protein sources before vs after a 12-h ruminal in situ digestion were similar (P greater than .10). In a growth study, a basal diet of 80% ensiled corncobs and 20% alfalfa was fed to 60 individually fed crossbred steers (215 kg BW). Steers were supplemented with U, BM, Fth, 1/2 BM:1/2 Fth, 1/2 BM:1/2 CGM and 1/3 BM:1/3 Fth:1/3 CGM (protein basis). Protein sources were fed at 30, 45 and 60% of the supplemental N with urea supplying the remainder. Protein efficiency was calculated using the slope ratio technique. Protein efficiency was similar (P greater than .10) for BM- and Fth-supplemented calves. Protein efficiencies were similar (P greater than .10) for BM:CGM, BM:Fth and BM:Fth:CGM combinations. These data indicate the Fth is a digestible high escape protein source that is useful in diets for growing ruminants.  相似文献   

19.
In four trials, steer calves were received in the feedlot, processed and fed diets supplemented with soybean meal (SBM), 1% urea (UR) or 1% urea plus sarsaponin (S) over a 28-d period. In trials 1 and 2, the feeding period was extended to approximately 62 d, in which steers were fed a common (SBM) diet the last 34 d. In trials 3 and 4, a SBM plus S diet treatment was included. During the first 28 d (four trial summary) daily gains of steers fed urea plus S (.74 kg) were intermediate to and significantly different from gains of steers fed SBM (.84 kg) or UR (.66 kg) diets. However, at the end of the 62-d feeding period (two trial summary) daily gains, feed intakes and feed efficiency did not differ (P greater than .05) among treatments. No significant improvements in performance were found in steers fed SBM diets supplemented with S. In swine trials, pigs were fed diets containing no additive, 63 mg S X kg-1, 55 mg chlortetracycline (C) X kg-1 or S plus C in a grower-finisher (GF) and grower (G) trial. In the GF trial, overall efficiency of feed conversion was improved (P less than .05) by feeding S or S plus C. In the G trial, daily gains and intakes were greatest for pigs fed S plus C and differed (P less than .1) from those of pigs fed S or C in the diet. Compared with feeding S or C alone, gain and intake of growing pigs were stimulated to a greater extent when S was fed in combination with C. Feeding S with or without C improved efficiency of feed conversion in finishing pigs.  相似文献   

20.
Four rumen-fistulated steers averaging 400 kg in body weight were used in a 4 X 4 Latin square arrangement with 18-d periods to investigate the effect of treating soybean meal (SBM) with formaldehyde on nitrogen (N) utilization and ruminal fermentation. Experimental diets, on a dry matter basis, consisted of 42% corn silage, 48.5% cracked corn-mineral mixture and 9.5% SBM treated with 0, .3, .6 or .9% formaldehyde by weight. Dry matter and organic matter digestibilities were not affected by treatment. Formaldehyde treatment of SBM resulted in a linear decrease in N digestibility (P less than .005) and urinary N excretion (P less than .01) and a quadratic increase (P less than .05) in N retention. The depression in apparent N digestibility was small when SBM was treated with .3% formaldehyde. This level of formaldehyde treatment also had little effect on in vitro enzymatic hydrolysis of SBM. Ruminal ammonia-N concentrations were lower (P less than .05) in steers fed formaldehyde-treated SBM. Ruminal pH was lower (P less than .05) at 6 and 8 h postfeeding while volatile fatty acid concentrations were higher (P less than .05) at 8 and 12 h postfeeding for steers fed untreated SBM. Propionic acid (mol/100 mol) decreased linearly (P less than .05) with increasing level of formaldehyde treatment. Urea-N concentrations in plasma were decreased (P less than .001) and plasma-free essential amino acid concentrations were increased (P less than .10) by formaldehyde treatment. Ruminal disappearance of N from polyester bags containing the SBM supplements was greatly reduced (P less than .005) by formaldehyde treatment.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

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