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1.
This study aimed to validate the use of an automated chemiluminescent immunoassay analyser for salivary testosterone measurements in growing pigs and study how circadian pattern during daytime and stress can influence its values. The test method had intra- and inter-assay coefficient of variation lower than 10%. The method showed good linearity and recovery, and detection limits were low enough to detect salivary testosterone levels. No significant differences were observed in testosterone concentrations at different sampling time, and age and gender did not influence circadian pattern. In addition, this assay was used to quantify testosterone in two models of acute stress and, in both cases, significant increases (P < 0.01) in salivary testosterone were detected. Therefore, the automated assay system tested for porcine testosterone determinations would be suitable for its use in saliva samples and, furthermore, salivary testosterone levels could be used as a possible marker of acute stress in pigs. 相似文献
2.
Maternal stress in pregnant sows may induce long-lasting alterations in the behavior, physiology, and immunity of their offspring. The aim of the present study was to investigate the consequences of repeated social stress during late gestation on determinants of the hypothalamic–pituitary–adrenal axis and on hippocampal neurotransmitter profiles in pig offspring. All pregnant gilts were housed in pairs. Each Stress gilt was mixed with an unfamiliar gilt twice a week between days 77 and 105 of gestation (n = 18). Control gilts were housed in stable pairs over the same period (n = 18). Plasma cortisol and corticosteroid binding globulin (CBG) were measured in 1 male and 1 female per litter in a basal situation on postnatal days (PND) 4, 26, and 60 and in a stressful situation at PND 28 (2 d after weaning) and 62 (2 d after relocation to a new building). Prenatal stress had no effect on plasma cortisol, but it decreased CBG at PND 26. Brain and adrenals were collected from 1 female per litter after weaning or relocation at PND 28 and PND 62. Adrenals were additionally collected at PND 4. Glucocorticoid receptor binding in the hippocampus and hypothalamus was not affected by prenatal treatment. However, prenatal stress increased the expression of 11β-hydroxysteroid dehydrogenase type 1 mRNA in the hippocampus after weaning (P < 0.05) and after relocation (P = 0.08). In addition, prenatally stressed piglets showed an increased 5-hydroxyindole-3-acetic acid to 5-hydroxytryptamine ratio in the hippocampus after weaning and increased hippocampal c-fos mRNA expression and noradrenaline concentration after relocation (P < 0.05). Prenatal stress also increased the relative adrenal weight at PND 4 and the cell density in the cortex and the medulla at PND 28, whereas no difference was found for activities of catecholamine-synthesising enzymes in the medulla. Overall, our data indicate that repeated social stress during pregnancy has long-lasting consequences on hypothalamic–pituitary–adrenal axis and hippocampal neurotransmitter activity in the offspring of pigs. 相似文献
3.
Exposure to high temperatures during the summer renders pigs susceptible to severe heat stress. Our previous studies found that pig small intestine epithelial tissue became significantly damaged following exposure to heat stress, negatively affecting body weight gain. The deleterious effects of heat stress could be ameliorated using a traditional Chinese medicine decoction (CMD), sustaining normal growth while under heat stress. In the current study, we hypothesized the mechanism of CMD activity to be via regulation of gut hormones (NPY, MLN, SCT and GCG) secretion from endocrine cells, which are responsible for nutrient digestion and absorption. To test this, 36 Chinese experimental mini-pigs (2 months of age) were screened according to weight and litter origin, and divided into three treatment groups: control (23 °C for 24 h + standard feed), heat stress (HS; 26 °C for 19 h, 40 °C for 5 h + standard feed) and CMD (26 °C for 19 h, 40 °C for 5 h + standard feed supplemented with CMD); n = 12 per group. Feed intake and body weight gain were measured daily. Pigs were euthanized at days 1 and 6 after initial treatment with blood and sections of small intestine epithelial tissue collected. Serum cortisol (Cor) concentrations were determined using RIA. Endocrine cell number and structural analysis were performed using silver staining, and gut hormone secretion examined by microarray. Dietary supplementation with CMD significantly improved porcine growth performance (P < 0.05), decreased the Cor levels (P < 0.01), increased endocrine cell number as well as up-regulating neuropeptide Y (NPY), motilin (MLN) and secretin (SCT) and down-regulating glucagon (GCG) expression in pig jejunum on day 6 when compared with the HS group. Taken together, our results indicate CMD supplementation can significantly reduce the negative effects of heat stress on pig jejunum, maintaining growth performance similar to non-heat stressed animals. CMD's activity appears to be via adjusting gut hormone secretion to regulate metabolism and improve animal growth. 相似文献
4.
The assessment of ridden horse behavior by 12 equestrian professionals (riding instructors n = 4, riders n = 4, veterinarians n = 4) was compared with observed behavior and physiological measures (salivary cortisol and eye temperature). Horses (n = 10) were ridden at walk, trot, and canter in a predefined test of approximately 2-3 minutes. Video footage of the ridden test (RT) was analyzed using Observer XT 10 and duration of behavioral states/events recorded. Saliva was collected in the stable, after the warm-up (WU) and at 0, 5, 15, 30, and 60 minutes after the RT. The saliva was analyzed for cortisol (enzyme-linked immunosorbent assay) and the difference between minimum and maximum concentration (ng/mL) and associated sample times recorded. Eye temperature was measured using an infrared thermal camera (MobIR M8), static images (stable, after WU, after RT), and video footage (WU and RT) with maximum eye temperatures derived from set intervals. Mean maximum eye temperatures during ridden work were calculated. Video footage of the RT was observed by the 12 equestrian professionals who each scored the horses on 7 performance parameters derived from the Fédération Equestre Internationale rules for dressage events and the training scale of the German National Equestrian Federation (relaxation, energy, compliance, suppleness, confidence, motivation, and happiness). These scores were compared with behavioral and physiological measures and correlations investigated (Spearman's rank order correlation). Higher percentage durations of high head carriage (ranging from 0 to 50.75% of RT) and the nose carried at an angle in front of the vertical (0%-74.29% of RT) correlated with overall less favorable assessment by the equestrian professionals (P < 0.05) and only the instructors associated neutral head carriage (32.76%-91.92% of RT) and vertical nasal angle (0.97%-68.90% of RT) as a positive sign (P = 0.03 and P = 0.04, respectively). Increases in salivary cortisol positively correlated with the duration of low head carriage (P < 0.05), suggesting that this way of going increased the demands placed on the horse. Increased eye temperature positively correlated with duration of nose carried behind the vertical when ridden (P = 0.02) and negatively correlated with duration of nose carried in front of the vertical (P = 0.01). Some discrepancy between physiological evidence and professional assessment of ridden horse behavior was evident as were differences between groups of professionals. Further evaluation of the association between behavioral signs and physiological measures is now required to ensure that the assessment of ridden horse performance is based on valid and consistent measures. 相似文献
5.
The specialization of swine production and the market demand for pigs at different growth stages makes road transportation inevitable. However, road transportation usually causes a stress response in pigs. It is reported that homeopathic remedies supplementation could alleviate the stress response in pigs. This study investigated the effects of road transportation with or without homeopathic remedy (Convermax) supplementation on growth performance, nutrient digestibility, fecal microbiota, and serum cortisol and superoxide dismutase (SOD) concentrations in growing pigs. A total of 180 crossbred 49-d-old growing pigs [(Yorkshire × Landrace) × Duroc] with an initial body weight of 13.17 ± 0.02 kg were randomly allotted to 2 groups based on the initial body weight, containing 18 replicates with 5 pigs (mixed sex) in each. The pigs were fed dietary supplementation of a homeopathic remedy (Convermax) (0 or 200 mg/kg of feed, as-fed) for 35 d. On day 21, 45 pigs (70-d old; 25.25 ± 0.37 kg) were randomly selected from each group and assigned to either 2 hr of road transportation or no road transportation, resulting in a 2 × 2 factorial design. We found that road transportation led to an increase in the fecal coliform bacteria counts (P = 0.023) and serum cortisol concentration (P = 0.039) and a decrease in the serum SOD concentration (P < 0.001). However, supplementing homeopathic remedy (Convermax) to the diet of growing pigs increased gain to feed ratio (P = 0.042), apparent nitrogen digestibility (P = 0.019), and serum SOD concentration (P = 0.007), whereas decreased serum cortisol concentration (P = 0.022). In brief, road transportation induced stress response for growing pigs and increased harmful bacteria counts in their intestines. Dietary supplementation of homeopathic remedy (Convermax) alleviated stress response, improved apparent nitrogen digestibility, and increased gain to feed ratio. However, no significant interactive effects between road transportation with or without dietary homeopathic remedy (Convermax) levels were observed on the detected parameters in growing pigs. 相似文献
6.
Changes in pig salivary cortisol in response to transport simulation, food and water deprivation, and mixing 总被引：1，自引：0，他引：1
Cortisol concentrations in the saliva of two groups (N = 5/group) of prepubertal pigs were measured by radioimmunoassay. Samples were collected in the home pen under normal husbandry conditions and after a 24 h period when food and water were withheld. The pigs were then transferred to a transport simulator, which was left stationary (control) or set in motion (experimental), and further samples were taken 1 h later before the animals returned to their pens. In the following week, the two groups of pigs were mixed and saliva was collected over a 3 h period. Samples were also taken 2 days later after the pigs had been injected with a maximally stimulating dose of adrenocorticotrophin (ACTH). Transport simulation in deprived animals, and mixing, produced salivary cortisol levels similar to those seen after ACTH. Food and water deprivation alone also increased cortisol secretion whereas transport simulation in non-deprived animals had no effect. These results indicate that salivary cortisol estimation offers a non-invasive means of measuring stress responses in unrestrained pigs. 相似文献
7.
Domestic animals are often repeatedly exposed to the same anthropogenic stressors. Based on cortisol secretion and heart rate, it has been demonstrated that transport is stressful for horses, but so far, changes in this stress response with repeated road transport have not been reported. We determined salivary cortisol concentrations, fecal cortisol metabolites, cardiac beat-to-beat (RR) interval, and heart rate variability (HRV) in transport-naive horses (N = 8) transported 4 times over a standardized course of 200 km. Immunoreactive salivary cortisol concentrations always increased in response to transport (P < 0.001), but cortisol release decreased stepwise with each transport (P < 0.05). Concentrations of fecal cortisol metabolites increased from 55.1 ± 4.6 ng/g before the first transport to 161 ± 17 ng/g the morning after (P < 0.001). Subsequent transport did not cause further increases in fecal cortisol metabolites. In response to the first transport, mean RR interval decreased with loading of the horses and further with the onset of transport (1551 ± 23, 1304 ± 166, and 1101 ± 123 msec 1 d before, immediately preceeding, and after 60–90 min of transport, respectively; P < 0.05). Decreases in RR interval during subsequent transports became less pronounced (P < 0.001). Transport was associated with a short rise in the HRV variable standard deviation 2 (P < 0.001 except transport 1), indicating sympathetic activation. No consistent changes were found for other HRV variables. In conclusion, a transport-induced stress response in horses decreased with repeated transport, indicating that animals habituated to the situation, but an increased cortisol secretion remained detectable. 相似文献
8.
A.V. Weschenfelder S. Torrey N. Devillers T. Crowe A. Bassols Y. Saco M. Piñeiro L. Saucier L. Faucitano 《Livestock Science》2013,157(1):234-244
The objective of this study was to evaluate the effects of trailer design on the stress responses and meat quality traits of 3 different pig crosses: 50% Pietrain breeding with HALNn (50Nn), 50% Pietrain breeding with HALNN (50NN) and 25% Pietrain breeding with HALNN genotype (25NN). Market barrows (n=360), as a subset of 12 trailer loads of pigs, were transported from farm to slaughter on 6 dates in 2009 for 45 min in either a pot-belly (PB) or flat-deck (FD) trailer, with 120 pigs/genetic group being represented. Temperature (T) and relative humidity (RH) were recorded by data loggers mounted in both trailers. Behaviours and handler interventions were video-taped at loading, unloading and in lairage. At exsanguination, blood samples were collected for the assessment of lactate, cortisol, creatine kinase (CK), haptoglobin and Pig-MAP concentrations. Meat quality was measured in the longissimus dorsi (LD), semimembranosus (SM) and adductor (AD) muscles of all pigs. Temperatures were warmer in compartments 6 and 11 at loading (P<0.001), compartment 11 during travelling (P=0.05), and in compartment 5 at unloading (P=0.01) of the PB trailer. Pigs unloaded from the FD trailer overlapped more (P<0.001), whereas (P<0.001) the frequency of jamming was noted for pigs unloaded from the PB trailer. Pigs with 50% Pietrain genetics overlapped and jammed more (P<0.001) than pigs with 25% Pietrain genetics, regardless of HAL status. Greater (P=0.03) CK levels were found in 50Nn pigs transported in the PB trailer, while 50Nn and 50NN pigs had greater (P=0.028) lactate levels than 25NN pigs. Carcasses from 50Nn and 50NN pigs were leaner (P=0.04), and the skin damage score was lower (P=0.04) in 25NN carcasses. Overlap-type bruises were greater (P=0.02) in pigs transported in the FD trailer. Pigs transported in the PB trailer had greater (P=0.05) pHu in the SM and AD muscles (P=0.013). Except for pHu in the SM muscle, all meat quality parameters were affected by the Hal gene (P=0.04). The use of a PB trailer for short distance transportation of pigs to slaughter negatively affected stress responses and meat quality. The greater proportion of Pietrain genetics in the selection resulted in leaner carcasses, but also in pigs being more difficult to handle. Crossbreeding appeared to have a greater impact on animal welfare and meat quality than vehicle type, but trailer type may emphasize these negative genotype-related defects. 相似文献
9.
Effects of supplemental L-tryptophan on serotonin, cortisol, intestinal integrity, and behavior in weanling piglets 总被引：2，自引：0，他引：2
Koopmans SJ Guzik AC van der Meulen J Dekker R Kogut J Kerr BJ Southern LL 《Journal of animal science》2006,84(4):963-971
Stress occurs in intensive pig farming when piglets are weaned and mixed. In this study, we investigated whether this stress might be reduced with elevated dietary levels of Trp. The effects of supplemental dietary Trp (5 g/kg of feed, as-fed basis) were tested on the neuroendocrine system, intestinal integrity, behavior, and growth performance in nursery pigs, both before and after mixing. Mixing occurred 5 d after weaning and diet introduction. On d 4, 5, and 6, Trp-fed pigs vs. control pigs showed approximately a 2-fold elevation in plasma Trp concentrations (68 +/- 7 vs. 32 +/- 2 micromol/L; P < 0.001), a 38% increase in hypothalamic serotonin turnover as measured by 5-hydroxyindoleacetic acid:5-hydroxytryptamine (P < 0.001), and an 11 to 18% increase (P < 0.05) in the intestinal villus height:crypt depth. Before (d 4) and at (d 5) mixing, saliva but not plasma cortisol concentrations were reduced (P < 0.02) by approximately 2-fold in Trp-fed pigs vs. control pigs. Intestinal paracellular (horseradish peroxidase) and transcellular (fluorescein isothiocyanate) transport of macromolecules were not affected by dietary treatment, but mixing induced a 2-fold reduction (P < 0.05) in transcellular transport. Behavioral responses (lying and standing) at mixing were not affected by dietary treatment, except on d 10 after diet introduction when Trp supplementation induced more lying and less standing (P < 0.02). Average daily gain and ADFI were not different among dietary groups (P > 0.10). In conclusion, supplemental dietary Trp (5 g/kg) to piglets increased hypothalamic serotonergic activity, reduced the salivary cortisol response to mixing, improved intestinal morphology, and reduced physical activity 10 d after diet introduction. Consequently, diets containing high Trp levels improved neuroendocrine components of stress and increased gastrointestinal robustness but did not affect behavioral reactivity in nursery pigs during weaning and mixing. 相似文献
10.
This study was conducted to evaluate the effects of different stocking densities on growth performance, blood parameters, intestinal morphology and intestinal immunity of growing pigs. A total of 288 male pigs (44.35 ± 0.50 kg) were randomly assigned to groups with stocking densities of 2.46, 1.23 and 0.82 m2/pig for a month. The results showed that there was no significant difference on growth performance among groups. Pigs in the stocking density of 0.82 m2/pig had the lowest backfat thickness and spleen weight index among groups (P < 0.05). With increasing stocking density, the serum concentrations of blood urea nitrogen (BUN), transglutaminase (TGG), alkaline phosphatase (AKP) and immunoglobulin A (IgA) were increased, and albumin (ALB), albumin-to-globulin ratio (ALB:GLO), insulin-like growth factor-1 (IGF-1) and immunoglobulin (IgM) were decreased (P < 0.05), and cortisol tended to increase and glucose tended to decrease (0.05 < P < 0.1). Compared with the stocking density of 2.46 m2/pig, the ileal villus height and jejunal villus width decreased in stocking densities of 1.23 and 0.82 m2/pig (P < 0.05). The duodenal villus height and ileal villus width in the stocking density of 0.82 m2/pig were the lowest among 3 groups (P < 0.05). The content of immunoglobulin A in duodenum, jejunum and ileum mucosa increased along with increasing density (P < 0.05). The contents of interleukin (IL)-2 in the spleen or liver and IL-10 in the spleen were higher in the stocking density of 0.82 m2/pig than in other 2 groups. These results showed that stocking density could affect the metabolism, intestinal morphology, and immunity of growing pigs and 1.23 m2/pig may be the suitable stocking density for the growing pigs in the present study. 相似文献
11.
Rafael A Cabrera James L Usry Consuelo Arrellano Eduardo T Nogueira Marianne Kutschenko Adam J Moeser Jack Odle 《畜牧与生物技术杂志(英文版)》2013,4(1):29
Background
Creep feeding is used to stimulate piglet post-weaning feed consumption. L-Glutamine (GLN) is an important source of fuel for intestinal epithelial cells. The objective of this study was to determine the impact of creep feeding and adding GLN or AminoGut (AG; containing glutamine + glutamate) to pre- and post-weaning diets on pig performance and intestinal health. Litters (N = 120) were allotted to four treatments during 14–21 d of lactation: 1) No creep feed (NC, n = 45); 2) creep fed control diet (CFCD, n = 45); 3) creep fed 1% GLN (CFGLN, n = 15); 4) creep fed .88% AG (CFAG, n = 15). After weaning, the NC and CFCD groups were sub-divided into three groups (n = 15 each), receiving either a control nursery diet (NC-CD, CFCD-CD) or a diet supplemented with either GLN (NC-GLN, CFCD-GLN) or with AG (NC-AG, CFCD-AG). Litters that were creep fed with diets containing GLN or AG also were supplemented with those amino acids in the nursery diets (CFGLN-GLN, CFAG-AG). Glutamine was added at 1% in all three post-weaning diet phases and AG was added at .88% in phase 1 and 2 and at .66% in phase 3.Results
Feed conversion (feed/gain) showed means among treatment means close to significance (P = 0.056) and Tukey’s test for pairwise mean comparisons showed that Pigs in the CFGLN-GLN group had the best feed conversion (feed/gain) in the first three-week period post-weaning, exceeding (P = 0.044) controls (CFCD-CD) by 34%. The NC-AG group had (P = 0.02) the greatest feed intake in the last three week of the study, exceeding controls (CFCD-CD) by 12%. CFGLN-GLN, CFCD-GLN and sow reared (SR) pigs had the greatest (P = 0.049) villi height exceeding the CFCD-AG group by 18%, 20% and 19% respectively. The CFAG-AG group had the deepest (P = 0.001) crypts among all treatments. CFGLN-GLN, CFCD-GLN and SR groups had the greatest (P = 0.001) number of cells proliferating (PCNA) exceeding those in the NC-CD group by 43%, 54% and 63% respectively. Sow reared pigs showed the greatest (P = 0.001) intestinal absorption capacity for xylose and mannitol.Conclusion
Supplementation of creep feed and nursery diets with GLN and/or AminoGut in the first three week improved feed conversion possibly due to improved intestinal health. 相似文献12.
Blood and saliva samples were taken from groups of pigs maintained in intensive conditions. Multiple samples were taken from two unrestrained pregnant sows fitted with jugular cannulae. Single samples were taken from groups (mixed gilts and entire males; 70–90 kg) which were lightly exercised (7) or restrained (12). The rate of salivary secretion was low and collection of adequate samples took 5 min; in a number of pigs no saliva could be obtained.In order to stimulate salivary secretion, pigs (70–80 kg) were injected with pilocarpine nitrate (25 mg, subcutaneous) which produced a copious flow of saliva persisting for at least 15 min.Resting sows had higher mean levels of cortisol in plasma, ultrafiltrate and saliva than the other groups, which did not differ from each other. Within the pilocarpine group, males had a higher ultrafiltrate level of cortisol than females. In most instances salivary cortisol was significantly greater than ultrafiltrate cortisol.Ultrafiltrate and plasma cortisol were highly correlated (r=0.883) but this correlation was low in the presence of pilocarpine (r=0.260). Salivary cortisol was poorly correlated with either plasma (r=0.167) or ultrafiltrate cortisol (r=0.278) and the correlation with plasma was even lower following the administration of pilocarpine (r=0.086).It was concluded that salivary estimates of cortisol in the pig were not usefully correlated with levels of ultrafiltrate (free) cortisol. 相似文献
13.
Lay DC Kattesh HG Cunnick JE Daniels MJ Kranendonk G McMunn KA Toscano MJ Roberts MP 《Journal of animal science》2011,89(6):1787-1794
Sows subjected to prenatal stress have been found to produce offspring that have altered responses to stress. Our objective was to determine if exposing a sow to stress would alter the response of the offspring to lipopolysaccharide (LPS) at 2 mo of age or their response to mixing stress at 4 mo of age. Sow treatments consisted of intravenous injections of ACTH (1 IU/kg of BW), exposure to rough handling for a 10-min duration (rough), or no treatment (control) once per week from d 42 to 77 of gestation. At 2 mo of age, pigs from each treatment, 1 per litter (n = 21, 17, and 15 for the ACTH, rough, and control treatments, respectively), were challenged with 2 μg of LPS/kg of BW or saline, or served as a noninjected control. Their behavioral response to a human approach test and salivary cortisol were measured. At 4 mo of age, 1 pig from each treatment (n = 14, 14, and 15 for the ACTH, rough, and control treatments, respectively) was taken from its home pen and placed in a pen of unfamiliar pigs. At this time, a punch biopsy wound (6 × 6 mm) was created to measure the ability of the pig to heal the wound. At this same time, each pig received a 1-mL intramuscular injection of 20% ovine red blood cells (oRBC), and then a second injection of oRBC at 21 d postmixing. Blood samples were collected 3 times per week for 2 wk and then once a week for 4 more weeks. Blood samples were analyzed for cortisol, porcine corticosteroid-binding globulin, antibody response to oRBC, and nitric oxide production by macrophages. Behavior was recorded during the first 5 d after mixing. All pigs in the LPS challenge responded with characteristic sickness behavior; however, pigs in the rough treatment showed less sickness behavior than those in the other 2 treatments (P < 0.05). Maternal stress treatment did not affect (P < 0.43) salivary cortisol. Pigs from all treatments responded similarly to mixing stress with regard to cortisol, porcine corticosteroid-binding globulin, antibody titers, nitric oxide production, and hematology measures, and all pigs experienced the same amount of aggression in response to mixing. Without altering peripheral measures of stress responsivity, prenatal stress enhanced the ability of pigs to cope with a simulated immune challenge, which could prove to be an adaptation to challenging environments. 相似文献
14.
Co-mingling weaned pigs often results in aggressive behavior which can result in injury and reduced performance. This experiment examined the effect of Acclimate™, a product reported to reduce equine aggression, on weaned pig aggression and growth performance. Weanling pigs (n=214, avg. wt.=5.93 kg) were blocked by weight, sex and litter (n=25) and randomly assigned to either Acclimate™ treatment or a control group. Acclimate™ treated pigs were marked on the nose, face and neck. Pigs were individually identified and videotaped to determine frequency of aggressive sequence (FAS) and duration of aggression (DOA) on an individual pig basis. Pig weights and feed consumption were used to determine feed intake, total weight gain (TWG), average daily gain (ADG) and feed to gain ratio (F/G). Differences between litter, sex and treatment for FAS, DOA, F/G and ADG were tested using GLM, SAS. Neither Acclimate™ treatment or sex had a significant effect on FAS, TWG or F/G. However, there was a trend towards reduced duration and intensity of aggression in Acclimate™ treated pigs (p=0.1). There was a significant effect of litter (p<0.01) and TWG (p<0.001) on FAS at both 1 and 3 weeks. Acclimate™ treatment of weaned pigs does not decrease FAS, but may decrease the intensity and duration of aggression without negatively effecting growth. 相似文献
15.
P. K. Pathak R. Roychoudhury J. Saharia M. C. Borah D. J. Dutta R. Bhuyan D. Kalita 《Tropical animal health and production》2018,50(5):1025-1032
The present study was formulated to find out the status of important season related thermal stress biomarkers of pure-bred (Hampshire) and crossbred (50% Hampshire × 50% local) pigs under the agro-climatic condition of Assam State, India. The experiment was also aimed to study the role of different level of energy ration (110, 100, and 90% energy of NRC feeding standard for pig) in variation of physiological and biochemical parameters in two genetic groups of pigs in different seasons. The metabolizable energy value were 3260, 2936.5, and 3585.8 kcal/kg in grower ration and 3260.2, 2936.6, and 3587 kcal/kg in finisher ration for normal energy (NE), low energy (LE) and high energy (HE), respectively. Both the genetic group of animals were housed separately under intensive system of management. Each pen was measuring 10′ × 12′ along with an outer enclosure. Six weaned piglets (almost similar body weight of average 10.55 kg) of each group were kept in a separate pen. However, after attainment of 35 kg body weight, the animals of a group were divided in two pens of three animals each. The present experiment indicated that average ambient temperature during summer months (27.33–29.51 °C) was above the comfort zone for pigs (22 °C). The significantly (P?<?0.01) higher relative humidity (RH) (%) was recorded in outdoor environment (87.26–91.10%) and in the morning time (86.60–91.10%). The temperature humidity index (THI) during the study period was found to be indicative of thermal stress to the experimental animals during summer (79.55–82.56). Physiological parameters viz., respiration rate (RR) and rectal temperature (RT) were significantly (P?<?0.01) higher in summer season (43.75–72.12 breaths/min. and 102.29–103.23 °F) and non-significantly higher values were recorded in Hampshire pigs. It was also found that the significantly (P?<?0.01) lower RR as well as RT was recorded in the pigs fed with high energy (HE) ration during summer season. Serum triiodothyronine (T3) and thyroxine (T4) concentrations were significantly (P?<?0.01) lower during summer, while both the genetic groups showed significantly (P?<?0.01) higher concentration of serum cortisol during summer season. It was also observed that thyroid hormone and cortisol concentrations were maintained in groups of pig fed vegetable oil incorporated HE diet during summer. From the present study, it is found that the increasing the energy level of the ration might be helpful to minimize the effects of thermal stress during summer. 相似文献
16.
Despite a large amount of work on compensatory growth in pigs it continues to be poorly understood with many conflicting reports. The aim of this work was to conduct four similar trials using the same genotype of pig, facilities, feed and growth restriction period to determine whether it was possible to obtain consistent results using a constant trial set-up. A total of 576 pigs (Hampshire sire×(Large White×Landrace) dam) were used. Pigs were weaned onto trial at 26.8±0.11 (mean±SE) days of age at a mean weight of 8.1±0.07 kg and remained on trial until slaughter, approximately 150.9±0.66 days of age at a mean weight of 98.4±0.65 kg. In each of the four trials the restriction period was for 3 weeks immediately following weaning. Pigs received either a high (Control; 17.5 g/kg) or a low (weaner restrict (WR); 8.0 g/kg) lysine diet during these 3 weeks, all pigs then received a high lysine diet up until slaughter, 15.5 and 12.0 g/kg of lysine for the grower and finisher diets respectively. Pigs from trial 1 ate (P<0.001) and gained (P<0.001) more throughout the weaner stage than all other trials. Growth performance was successfully reduced during the weaner phase. WR pigs grew more slowly (P<0.001) and less efficiently (P<0.001) than Control pigs. WR pigs from trial 1 demonstrated compensatory gains throughout the grower stage, growing 6% faster than Control pigs from trial 1 due to an improvement in feed efficiency (P<0.001). WR pigs from trial 2 demonstrated compensatory gains during the finisher stage, increasing their rate of gain by 7.0% compared to Control pigs from trial 2 again due to an improvement in feed efficiency (P<0.1). However previous lysine restriction did not result in compensatory growth in trials 3 and 4. WR pigs from trials 3 (P<0.05) and 4 (P<0.1) had a lower feed intake compared to Control pigs during the grower stage and an overall lower lysine intake throughout the trial (P<0.05; P<0.05). Although pigs from trials 1 and 2 demonstrated compensatory growth following a reduction in performance when dietary lysine levels as low as 8.0 g/kg lysine were fed for a period of 3 weeks immediately post-weaning, pigs from experiments 3 and 4 did not, thus compensatory growth was not consistently observed. 相似文献
17.
《The Professional Animal Scientist》1999,15(1):7-13
The primary objective of this study was to determine the effects of supplemental dietary fat during lactation on sow BW, sow backfat thickness, sow feed consumption, litter size, and pig growth rate. Dietary treatments included 0, 3, 6, and 9% supplemental low acid yellow fat in a traditional corn-soybean meal basal lactation diet. A total of 160 Landrace and crossbred sows (approximately 40 per treatment) were included in the study. Sows fed 3 and 6% supplemental fat had greater (P<0.10) average backfat thickness at weaning. Sow weight change and feed consumption were inconsistent among dietary fat levels. Dietary fat level during lactation did not affect number of pigs born alive or number of stillborns. However, the 9% fat level was associated with more mummified pigs at birth. Number of pigs weaned was greater for the 0% supplemental fat than for the 9% fat level. The largest average pig weights at 21 (5.8±0.29 kg) and 28 (7.48±0.38) d of age were those from sows fed the 3% added fat diet. Sows with ≤25.4 mm backfat at farrowing had more pigs born alive (P<0.05), had less backfat at 21 and 28 d of lactation (P<0.05), and consumed more feed during wk 2 and 3 of lactation. Of all sows fed the control diet, sows with >25.4 mm backfat at farrowing consistently had heavier pigs throughout the lactation phase (P<0.05). Backfat loss during lactation was lower (P<0.05) for sows with ≤25.4 mm at farrowing within all dietary treatments. Consistent significant differences were not observed in sow weight loss or feed consumption between low and high backfat sows for each dietary treatment. Sow backfat loss during lactation is dependent on body condition at farrowing, in that, fatter sows at farrowing have greater backfat loss during lactation. Sows with ≤25.4 mm of backfat at farrowing responded to added dietary fat treatments and produced heavier pigs throughout the lactation period. 相似文献
18.
The effect of three dietary concentrations of Crotalaria pallida (C. pallida) seeds (0, 1, 2, and 3% w/w) of their normal diet were investigated in commercial laying hens during a 35 day feeding trial. All concentrations of C. pallida decreased body weight and feed intake (P < 0.05). Egg mass production and average egg weight were decreased by feeding of ≥2% C. pallida seeds (P < 0.05). All concentrations of C. pallida increased relative lung weight and serum activity of ALT, AST and LDH (P < 0.05); 3% C. pallida seeds decreased liver weight (P < 0.05). Analysis of the C. pallida seeds for dehydropyrrolizidine alkaloid content detected usaramine and its N-oxide at a total alkaloid concentration of 0.18% (dry weight). Usaramine was also detected in the eggs of all hens fed C. pallida seeds. 相似文献
19.
Van Der Staay FJ De Groot J Van Reenen CG Hoving-Bolink AH Schuurman T Schmidt BH 《Journal of veterinary pharmacology and therapeutics》2007,30(5):410-416
We assessed the efficacy of Butafosfan, a component of Catosal, in the metaphylactic treatment of stress in pigs. Four 6-week-old female littermates were taken from 12 litters. They were confronted with a pig from a different litter for 2 h. There were 24 pairs, each consisting of confronting two unfamiliar pigs in a new pen. This housing of unfamiliar pigs provides a good, but simple, model of the psychosocial stress that pigs experience when housed in large groups on pig farms. Immediately before being housed with an unfamiliar pig, 12 pairs of pigs were injected subcutaneously with Catosal at a dose equivalent to 20 mg Butafosfan per kg body weight; the other 12 pairs received the control solution containing all ingredients of Catosal except Butafosfan. The frequency and duration of aggressive behavior and the salivary cortisol response were measured during the first 2 h of the encounter. No adverse effects associated with Catosal were observed. Subcutaneous injection of Catosal reduced the stress-induced salivary cortisol response and the frequency of aggressive behavior evoked by the social stress of housing two unfamiliar pigs together. 相似文献
20.
Anna Valros Camilla Munsterhjelm Eero Puolanne Marita Ruusunen Mari Heinonen Olli A T Peltoniemi A Reeta P?s? 《Acta veterinaria Scandinavica》2013,55(1):75